196 results on '"Yenari MA"'
Search Results
2. Mechanisms and Potential Therapeutic Applications of Microglial Activation after Brain Injury
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Kim, JY, Kim, N, and Yenari, MA
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Pharmacology & Pharmacy ,Neurosciences ,Pharmacology and Pharmaceutical Sciences - Abstract
As the resident immune cells of the central nervous system, microglia rapidly respond to brain insults, including stroke and traumatic brain injury. Microglial activation plays a major role in neuronal cell damage and death by releasing a variety of inflammatory and neurotoxic mediators. Their activation is an early response that may exacerbate brain injury and many other stressors, especially in the acute stages, but are also essential to brain recovery and repair. The full range of microglial activities is still not completely understood, but there is accumulating knowledge about their role following brain injury. We review recent progress related to the deleterious and beneficial effects of microglia in the setting of acute neurological insults and the current literature surrounding pharmacological interventions for intervention.
- Published
- 2015
3. Regulation of inflammatory transcription factors by heat shock protein 70 in primary cultured astrocytes exposed to oxygen–glucose deprivation
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Kim, JY, Yenari, MA, and Lee, JE
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Biological Psychology ,Biomedical and Clinical Sciences ,Neurosciences ,Psychology ,Genetics ,Biotechnology ,1.1 Normal biological development and functioning ,Underpinning research ,Animals ,Astrocytes ,Brain Ischemia ,Encephalitis ,Glucose ,HSP70 Heat-Shock Proteins ,Heat-Shock Response ,I-kappa B Proteins ,JNK Mitogen-Activated Protein Kinases ,MAP Kinase Signaling System ,Mice ,Inbred ICR ,NF-kappa B ,Oxygen ,Phosphorylation ,Primary Cell Culture ,STAT1 Transcription Factor ,Transcription Factor AP-1 ,ischemic injury ,inflammation ,70-kDa heat shock protein ,transcription factors ,phosphorylation ,Cognitive Sciences ,Neurology & Neurosurgery ,Biological psychology - Abstract
Inflammation is an important event in ischemic injury. These immune responses begin with the expression of pro-inflammatory genes modulating transcription factors, such as nuclear factor-κB (NF-κB), activator protein-1 (AP-1), and signal transducers and activator of transcription-1 (STAT-1). The 70-kDa heat shock protein (Hsp70) can both induce and arrest inflammatory reactions and lead to improved neurological outcome in experimental brain injury and ischemia. Since Hsp70 are induced under heat stress, we investigated the link between Hsp70 neuroprotection and phosphorylation of inhibitor of κB (IκB), c-Jun N-terminal kinases (JNK) and p38 through co-immunoprecipitation and enzyme-linked immunosorbent assay (ELISA) assay. Transcription factors and pro-inflammatory genes were quantified by immunoblotting, electrophoretic-mobility shift assay and reverse transcription-polymerase chain reaction assays. The results showed that heat stress led to Hsp70 overexpression which rendered neuroprotection after ischemia-like injury. Overexpression Hsp70 also interrupts the phosphorylation of IκB, JNK and p38 and blunts DNA binding of their transcription factors (NF-κB, AP-1 and STAT-1), effectively downregulating the expression of pro-inflammatory genes in heat-pretreated astrocytes. Taken together, these results suggest that overexpression of Hsp70 may protect against brain ischemia via an anti-inflammatory mechanism by interrupting the phosphorylation of upstream of transcription factors.
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- 2015
4. Pharmacological induction of the 70-kDa heat shock protein protects against brain injury
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Kim, N, Kim, JY, and Yenari, MA
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Biological Psychology ,Biomedical and Clinical Sciences ,Psychology ,Neurosciences ,Physical Injury - Accidents and Adverse Effects ,Traumatic Brain Injury (TBI) ,Brain Disorders ,Traumatic Head and Spine Injury ,Neurological ,Animals ,Astrocytes ,Benzoquinones ,Brain ,Brain Hemorrhage ,Traumatic ,Brain Injuries ,Disease Models ,Animal ,HSP70 Heat-Shock Proteins ,Lactams ,Macrocyclic ,Male ,Mice ,Inbred C57BL ,Microglia ,Neurons ,Neuroprotective Agents ,Time Factors ,Treatment Outcome ,animal studies ,traumatic brain injury ,therapeutic approaches ,Cognitive Sciences ,Neurology & Neurosurgery ,Biological psychology - Abstract
The 70-kDa heat shock protein (HSP70) is known to protect the brain from injury through multiple mechanisms. We investigated the effect of pharmacological HSP70 induction in experimental traumatic brain injury (TBI). 3-month-old male C57/B6 mice were given 17-N-allylamino-17-demethoxygeldanamycin (17-AAG) intraperitoneally (IP, 2 mg/kg) or intracerebroventricularly (ICV, 1 μg/kg) to determine whether HSP70 could be induced in the brain. Mice were subjected to TBI via cortical controlled impact, and were treated with 17-AAG (or vehicle) IP according to one of two treatment regimens: (1) 2 mg/kg at the time of injury, (2) a total of three doses (4 mg/kg) at 2 and 1d prior to TBI and again at the time of injury. Brains were assessed for HSP70 induction, hemorrhage volume at 3 d, and lesion size at 14 d post-injury. Immunohistochemistry showed that both IP and ICV administration of 17-AAG increased HSP70 expression primarily in microglia and in a few neurons by 24 h but not in astrocytes. 17-AAG induced HSP70 in injured brain tissue as early as 6 h, peaking at 48 h and largely subsiding by 72 h after IP injection. Both treatment groups showed decreased hemorrhage volume relative to untreated mice as well as improved neurobehavioral outcomes. These observations indicate that pharmacologic HSP70 induction may prove to be a promising treatment for TBI.
- Published
- 2015
5. Innate inflammatory responses in stroke: mechanisms and potential therapeutic targets.
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Kim, JY, Kawabori, M, and Yenari, MA
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Biomedical and Clinical Sciences ,Immunology ,Brain Disorders ,Neurosciences ,Stroke ,Regenerative Medicine ,Aetiology ,2.1 Biological and endogenous factors ,Inflammatory and immune system ,Animals ,Blood-Brain Barrier ,Humans ,Immunity ,Innate ,Inflammation ,Signal Transduction ,Brain ischemia ,inflammation ,neuroprotection ,stroke ,Medicinal and Biomolecular Chemistry ,Biochemistry and Cell Biology ,Pharmacology and Pharmaceutical Sciences ,Medicinal & Biomolecular Chemistry ,Pharmacology and pharmaceutical sciences ,Medicinal and biomolecular chemistry - Abstract
Stroke is a frequent cause of long-term disability and death worldwide. Ischemic stroke is more commonly encountered compared to hemorrhagic stroke, and leads to tissue death by ischemia due to occlusion of a cerebral artery. Inflammation is known to result as a result of ischemic injury, long thought to be involved in initiating the recovery and repair process. However, work over the past few decades indicates that aspects of this inflammatory response may in fact be detrimental to stroke outcome. Acutely, inflammation appears to have a detrimental effect, and anti-inflammatory treatments have been been studied as a potential therapeutic target. Chronically, reports suggest that post-ischemic inflammation is also essential for the tissue repairing and remodeling. The majority of the work in this area has centered around innate immune mechanisms, which will be the focus of this review. This review describes the different key players in neuroinflammation and their possible detrimental and protective effects in stroke. A better understanding of the roles of the different immune cells and their temporal profile of damage versus repair will help to clarify more effective modulation of inflammation post stroke.
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- 2014
6. The 70 kDa heat shock protein protects against experimental traumatic brain injury
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Kim, JY, Kim, N, Zheng, Z, Lee, JE, and Yenari, MA
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Neurology & Neurosurgery ,Clinical Sciences ,Neurosciences - Abstract
Traumatic brain injury (TBI) causes disruption of the blood brain barrier (BBB) leading to hemorrhage which can complicate an already catastrophic illness. Matrix metalloproteinases (MMPs) involved in the breakdown of the extracellular matrix may lead to brain hemorrhage. We explore the contribution of the 70 kDa heat shock protein (Hsp70) to outcome and brain hemorrhage in a model of TBI. Male, wildtype (Wt), Hsp70 knockout (Ko) and transgenic (Tg) mice were subjected to TBI using controlled cortical impact (CCI). Motor function, brain hemorrhage and lesion size were assessed at 3, 7 and 14 days. Brains were evaluated for the effects of Hsp70 on MMPs.In Hsp70 Tg mice, CCI led to smaller brain lesions, decreased hemorrhage and reduced expression and activation of MMPs compared to Wt. CCI also significantly decreased right-biased swings and corner turns in the Hsp70 Tg mice. Conversely, Hsp70 Ko mice had significantly increased lesion size, worsened brain hemorrhage and increased expression and activation of MMPs with worsened behavioral outcomes compared to Wt. Hsp70 is protective in experimental TBI. To our knowledge, this is the direct demonstration of brain protection by Hsp70 in a TBI model. Our data demonstrate a new mechanism linking TBI-induced hemorrhage and neuronal injury to the suppression of MMPs by Hsp70, and support the development of Hsp70 enhancing strategies for the treatment of TBI. © 2013 Elsevier Inc.
- Published
- 2013
7. Influence of therapeutic hypothermia on regeneration after cerebral ischemia.
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Yenari, MA and Han, HS
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Animals ,Humans ,Brain Ischemia ,Treatment Outcome ,Hypothermia ,Induced ,Nerve Regeneration ,Neurogenesis - Abstract
The protective effect of therapeutic hypothermia in cerebral ischemia is well accepted in experimental models, and some clinical studies show that there is benefit in humans as well. Long-term observations in animal and clinical studies have documented recovery of neurological function following hypothermia treatment. Diminished damage by hypothermic protection should contribute to the recovery in many ways, but hypothermia appears to enhance regeneration of brain tissue as well. Since regeneration of the brain after damage initiates within hours and is active days and weeks after stroke, prolonged hypothermia might affect regenerative processes which have been documented to occur in these time frames. As there is a lack of data at the basic and clinical levels, the mechanism of neuroregeneration by hypothermia is unclear. Yet, we speculate that hypothermia enhances regeneration by positively influencing neurogenesis, angiogenesis, gliogenesis and synapse/circuit formation after stroke. In this chapter, we will provide up-to-date data from experimental studies and clinical reports on the effect of therapeutic hypothermia on neuroregeneration, with perspectives on future research.
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- 2013
8. Pharmacologic heat shock protein 70 induction confers cytoprotection against inflammation in gliovascular cells
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Kacimi, R and Yenari, MA
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Lipopolysaccharides ,Cultured ,Neurology & Neurosurgery ,Cell Survival ,Cells ,NF-kappa B ,Neurosciences ,Brain ,Endothelial Cells ,Nitric Oxide Synthase Type II ,microglia ,Nitric Oxide ,Cell Line ,Mice ,cytoprotection ,Gene Knockdown Techniques ,Microvessels ,Animals ,HSP90 ,HSP70 Heat-Shock Proteins ,HSP90 Heat-Shock Proteins ,HSP70 - Abstract
© 2015 Wiley Periodicals, Inc. The inhibition of the 90-kDa heat shock protein (HSP90) leads to upregulation of the 70-kDa-inducible HSP70. HSP70 has been previously shown to be neuroprotective and anti-inflammatory. Geldanamycin (GA) and other HSP90 inhibitors have emerged as promising therapeutic agents in cancer, presumably owing to their ability to upregulate HSP70. However, the effects of HSP90 inhibition in brain inflammation are still unclear. We investigate the effect of a panel of HSP90 inhibitors on endotoxin-activated microglia and eventual protection from brain-derived endothelial cells. Prior studies have shown that GA protects brain cells from oxidative stress. We show here that when astrocytes or microglial BV2 cells were pretreated with GA or other HSP90 inhibitors, endotoxin-induced cell death was reduced in cocultures of BV2 microglia and brain-derived endothelial cells (bEND.3). Endotoxin-stimulated BV2 cells led to increased nitric oxide (NO) and inducible nitric oxide synthase which was prevented by treatment with all HSP90 inhibitors. HSP90 inhibitors also prevented lipopolysaccharide (LPS)-induced BV2 cell death. We also found that HSP90 inhibition blocked nuclear translocation of nuclear factor kappa B and attenuated IκBα degradation, and inhibited LPS-activated JAK-STAT phosphorylation. We show that pharmacologic inhibition of HSP90 with subsequent HSP70 induction protects cells that comprise the cerebral vasculature against cell death owing to proinflammatory stimuli. This approach may have therapeutic potential in neurological conditions with an inflammatory component. GLIA 2015;63:1200-1212 Main Points: Activated microglia are toxic to brain derived endothelial cells. Pharmacologically inducing HSP70 by HSP90 inhibitors protects endothelial cells via an anti-inflammatory mechanism.
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- 2015
9. Microglial P2Y12 Deficiency/Inhibition Protects against Brain Ischemia
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Webster, CM, Hokari, M, McManus, A, Tang, XN, Ma, H, Kacimi, R, and Yenari, MA
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nervous system - Abstract
Objective:Microglia are among the first immune cells to respond to ischemic insults. Triggering of this inflammatory response may involve the microglial purinergic GPCR, P2Y12, activation via extracellular release of nucleotides from injured cells. It is also the inhibitory target of the widely used antiplatelet drug, clopidogrel. Thus, inhibiting this GPCR in microglia should inhibit microglial mediated neurotoxicity following ischemic brain injury.Methods:Experimental cerebral ischemia was induced, in vitro with oxygen-glucose deprivation (OGD), or in vivo via bilateral common carotid artery occlusion (BCCAO). Genetic knock-down in vitro via siRNA, or in vivo P2Y12 transgenic mice (P2Y12-/- or P2Y12+/-), or in vivo treatment with clopidogrel, were used to manipulate the receptor. Neuron death, microglial activation, and microglial migration were assessed.Results:The addition of microglia to neuron-astrocyte cultures increases neurotoxicity following OGD, which is mitigated by microglial P2Y12 deficiency (P
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- 2013
10. Temperature affects thrombolytic efficacy using rt-Pa and eptifibatide, an in vitro study: editorial commentary on Meunier et al., 2012
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Yenari, MA
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- 2012
11. Neuroprotective mechanisms of hypothermia in brain ischaemia.
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Yenari MA, Han HS, Yenari, Midori A, and Han, Hyung Soo
- Abstract
Cooling can reduce primary injury and prevent secondary injury to the brain after insults in certain clinical settings and in animal models of brain insult. The mechanisms that underlie the protective effects of cooling - also known as therapeutic hypothermia - are slowly beginning to be understood. Hypothermia influences multiple aspects of brain physiology in the acute, subacute and chronic stages of ischaemia. It affects pathways leading to excitotoxicity, apoptosis, inflammation and free radical production, as well as blood flow, metabolism and blood-brain barrier integrity. Hypothermia may also influence neurogenesis, gliogenesis and angiogenesis after injury. It is likely that no single factor can explain the neuroprotection provided by hypothermia, but understanding its myriad effects may shed light on important neuroprotective mechanisms. [ABSTRACT FROM AUTHOR]
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- 2012
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12. Significance of marrow-derived nicotinamide adenine dinucleotide phosphate oxidase in experimental ischemic stroke.
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Tang XN, Zheng Z, Giffard RG, Yenari MA, Tang, Xian N, Zheng, Zhen, Giffard, Rona G, and Yenari, Midori A
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ANIMAL experimentation ,ANIMALS ,BONE marrow ,BRAIN ,CEREBRAL ischemia ,ENZYME inhibitors ,GLYCOPROTEINS ,KETONES ,MICE ,OXIDOREDUCTASES ,RESEARCH funding ,STROKE ,TREATMENT effectiveness ,CHEMICAL inhibitors ,PHARMACODYNAMICS - Abstract
Objective: Reperfusion after stroke leads to infiltration of inflammatory cells into the ischemic brain. Nicotinamide adenine dinucleotide phosphate oxidase (NOX2) is a major enzyme system that generates superoxide in immune cells. We studied the effect of NOX2 derived from the immune cells in the brain and in blood cells in experimental stroke.Methods: To establish whether NOX2 plays a role in brain ischemia, strokes were created in mice, then mice were treated with the NOX2 inhibitor apocynin or vehicle and compared to mice deficient in NOX2's gp91 subunit and their wild-type littermates. To determine whether NOX2 in circulating cells versus brain resident cells contribute to ischemic injury, bone marrow chimeras were generated by transplanting bone marrow from wild-type or NOX2-deficient mice into NOX2 or wild-type hosts, respectively.Results: Apocynin and NOX2 deletion both significantly reduced infarct size, blood-brain barrier disruption, and hemorrhagic transformation of the infarcts, compared to untreated wild-type controls. This was associated with decreased matrix metalloproteinase 9 expression and reduced loss of tight junction proteins. NOX2-deficient mice receiving wild-type marrow had better outcomes compared to the wild-type mice receiving wild-type marrow. Interestingly, wild-type mice receiving NOX2-deficient marrow had even smaller infarct sizes and less hemorrhage than NOX2-deficient mice receiving wild-type marrow.Interpretation: This indicates that NOX2, whether present in circulating cells or brain resident cells, contributes to ischemic brain injury and hemorrhage. However, NOX2 from the circulating cells contributed more to the exacerbation of stroke than that from brain resident cells. These data suggest the importance of targeting the peripheral immune system for treatment of stroke. [ABSTRACT FROM AUTHOR]- Published
- 2011
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13. Hyperglycemia promotes tissue plasminogen activator-induced hemorrhage by Increasing superoxide production.
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Won SJ, Tang XN, Suh SW, Yenari MA, Swanson RA, Won, Seok Joon, Tang, Xian Nan, Suh, Sang Won, Yenari, Midori A, and Swanson, Raymond A
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FIBRINOLYTIC agents ,ANIMAL experimentation ,ANTIOXIDANTS ,BIOLOGICAL models ,BLOOD sugar ,BLOOD-brain barrier ,CEREBRAL hemorrhage ,GLUCOSE ,HYPERGLYCEMIA ,INJECTIONS ,INTRAVENOUS therapy ,KETONES ,PEROXIDES ,RATS ,REPERFUSION ,RESEARCH funding ,STROKE ,SWEETENERS ,TISSUE plasminogen activator ,TREATMENT effectiveness ,DISEASE complications ,PHARMACODYNAMICS - Abstract
Objective: Risk of intracerebral hemorrhage is the primary factor limiting use of tissue plasminogen activator (tPA) for stroke. Clinical studies have established an association between admission hyperglycemia and the risk of hemorrhage with tPA use, independent of prior diabetes. Here we used an animal model of tPA-induced reperfusion hemorrhage to determine if this clinical association reflects a true causal relationship.Methods: Rats underwent 90 minutes of focal ischemia, and tPA infusion was begun 10 minutes prior to vessel reperfusion. Glucose was administered during ischemia to generate blood levels ranging from 5.9 ± 1.8mM (normoglycemia) to 21 ± 2.3mM. In some studies, apocynin was administered to block superoxide production by nicotinamide adenine dinucleotide phosphate (NADPH). Brains were harvested 1 hour or 3 days after reperfusion to evaluate the effects of hyperglycemia and apocynin on oxidative stress, blood-brain barrier breakdown, infarct volume, and hemorrhage volume.Results: Rats that were hyperglycemic during tPA infusion had diffusely increased blood-brain barrier permeability in the postischemic territory, and a 3- to 5-fold increase in intracerebral hemorrhage volumes. The hyperglycemic rats also showed increased superoxide formation in the brain parenchyma and vasculature during reperfusion. The effects of hyperglycemia on superoxide production, blood-brain barrier disruption, infarct size, and hemorrhage were all attenuated by apocynin.Interpretation: These findings demonstrate a causal relationship between hyperglycemia and hemorrhage in an animal model of tPA stroke treatment, and suggest that this effect of hyperglycemia is mediated through an increase in superoxide production by NADPH oxidase. [ABSTRACT FROM AUTHOR]- Published
- 2011
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14. Microglia potentiate damage to blood-brain barrier constituents: improvement by minocycline in vivo and in vitro.
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Yenari MA, Xu L, Tang XN, Qiao Y, Giffard RG, Yenari, Midori A, Xu, Lijun, Tang, Xian Nan, Qiao, Yanli, and Giffard, Rona G
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- 2006
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15. Reversal of early diffusion-weighted magnetic resonance imaging abnormalities does not necessarily reflect tissue salvage in experimental cerebral ischemia.
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Ringer TM, Neumann-Haefelin T, Sobel RA, Moseley ME, Yenari MA, Ringer, T M, Neumann-Haefelin, T, Sobel, R A, Moseley, M E, and Yenari, M A
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- 2001
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16. Therapeutic hypothermia for brain ischemia: where have we come and where do we go?
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Yenari MA, Hemmen TM, Yenari, Midori A, and Hemmen, Thomas M
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- 2010
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17. In cold blood: a new way to achieve therapeutic cooling?
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Yenari MA
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- Cold Temperature, Body Temperature Regulation, Skin Temperature
- Abstract
Competing Interests: Conflict of interest The author declares that she has no conflict of interest.
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- 2023
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18. Most Promising Approaches to Improve Stroke Outcomes: The Stroke Treatment Academic Industry Roundtable XII Workshop.
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Wechsler LR, Adeoye O, Alemseged F, Bahr-Hosseini M, Deljkich E, Favilla C, Fisher M, Grotta J, Hill MD, Kamel H, Khatri P, Lyden P, Mirza M, Nguyen TN, Samaniego E, Schwamm L, Selim M, Silva G, Yavagal DR, Yenari MA, Zachrison KS, Boltze J, and Yaghi S
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- Humans, Thrombolytic Therapy, Thrombectomy, Reperfusion, Treatment Outcome, Brain Ischemia therapy, Stroke drug therapy
- Abstract
The Stroke Treatment Academic Industry Roundtable XII included a workshop to discuss the most promising approaches to improve outcome from acute stroke. The workshop brought together representatives from academia, industry, and government representatives. The discussion examined approaches in 4 epochs: pre-reperfusion, reperfusion, post-reperfusion, and access to acute stroke interventions. The participants identified areas of priority for developing new and existing treatments and approaches to improve stroke outcomes. Although many advances in acute stroke therapy have been achieved, more work is necessary for reperfusion therapies to benefit the most possible patients. Prioritization of promising approaches should help guide the use of resources and investigator efforts., Competing Interests: Disclosures Dr Wechsler reports compensation from Astellas Pharma for consultant services; compensation from Brainsgate for consultant services; stock options in Forest Devices; compensation from Annals of Neurology for other services; and compensation from Athersys for consultant services. Dr Adeoye reports service as Chief Medical Officer for sense diagnostics and compensation from Nico Corporation for data and safety monitoring services. Dr Alemseged reports grants from the Heart Foundation; grants from Medical Research Future Fund; and grants from Sylvia and Charles Charitable Foundation. Dr Bahr-Hosseini reports a patent issued for Transcranial Electrical Stimulation in Stroke Early After Onset. Dr Deljkich reports stock options in Imperative Care, Inc, and employment by Imperative Care, Inc. Dr Fisher reports compensation from Simcere USA for consultant services; employment by Beth Israel Deaconess Medical Center; and compensation from Lumosa for consultant services. Dr Grotta reports compensation from Frazer, Ltd, for consultant services; compensation from Acticor for consultant services; and compensation from Prolong pharma for consultant services. Dr Hill reports grants from MicroVention, Inc; grants from Medtronic; employment by the University of Calgary; grants from Boehringer Ingelheim; grants from the Canadian Institutes of Health Research; and grants from NoNO, Inc. Dr Kamel reports compensation from the American Medical Association for consultant services; compensation from AstraZeneca for end point review committee services; compensation from Novo Nordisk for end point review committee services; compensation from Medtronic for other services; employment by Weill Cornell Medical College; an ownership stake in TET Medical; compensation from Boehringer Ingelheim for end point review committee services; compensation from Javelin Medical for consultant services; and compensation from Janssen Biotech for other services. Dr Khatri reports compensation from Shionogi, Inc, for consultant services; grants from Johnson & Johnson Health Care Systems, Inc; compensation from Bayer for consultant services; compensation from Lumosa for consultant services; compensation from Translational Sciences for other services; compensation from Basking Biosciences for consultant services; royalties from UptoDate, Inc, for online publication; and grants from the National Institutes of Health (NIH). Dr Lyden reports employment by the University of Southern California; compensation from NIH Clinical Center for other services; and compensation from Apex Innnovations for consultant services. Dr Mirza reports employment by Cerenovus. Dr Nguyen reports compensation from Idorsia for other services and compensation from Brainomix for consultant services. Dr Samaniego reports compensation from iSchemaView for consultant services; compensation from Rapid Medical for consultant services; compensation from Medtronic for consultant services; and compensation from MicroVention, Inc, for consultant services. Dr Schwamm reports compensation from Life Image for consultant services; compensation from Diffusion Pharmaceuticals for data and safety monitoring services; compensation from Medtronic for consultant services; service as member of board of directors for the American Heart Association; and compensation from Penumbra, Inc, for data and safety monitoring services. Dr Selim reports grants from NIH/National Institute on Aging; grants from NIH/National Institute of Neurological Disorders and Stroke; and compensation from MedRhythms, Inc, for consultant services. Dr Silva reports compensation from Pfizer for other services; employment by Sociedade Beneficente Israelita Brasileira Albert Einstein; compensation from ISchemaView for consultant services; compensation from Boehringer Ingelheim for consultant services; compensation from Bayer for consultant services; and employment by Universidade Federal de Sao Paulo. Dr Yavagal reports compensation from Gravity Medical Technology for consultant services; stock options in Poseydon; stock holdings in Athersys; compensation from Vascular Dynamics for consultant services; stock options in Rapid Medical; compensation from Poseydon for consultant services; compensation from Stryker Corporation for consultant services; compensation from Johnson & Johnson Health Care Systems, Inc, for consultant services; compensation from Medtronic USA, Inc, for consultant services; and compensation from Athersys for consultant services. Dr Yenari reports grants from NIH. Dr Zachrison reports grants from CRICO; compensation from Wolters Klewer Health, Inc, for other services; grants from the American College of Emergency Physicians; employment by Partners Healthcare; grants from the National Institute on Aging; and grants from the Society for Academic Emergency Medicine. Dr Boltze reports compensation from Astrocyte Pharmaceuticals for consultant services; compensation from Targed Biopharmaceuticals B.V. for consultant services; and employment by the University of Warwick. The other authors report no conflicts.
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- 2023
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19. Use of Botulinum Toxin for Limb Immobilization for Rehabilitation in Rats with Experimental Stroke.
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Zhang H, Liu J, Bingham D, Orr A, Kawabori M, Kim JY, Zheng Z, Lam TI, Massa SM, Swanson RA, and Yenari MA
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- Humans, Male, Rats, Animals, Forelimb, Paresis drug therapy, Botulinum Toxins therapeutic use, Stroke drug therapy, Stroke Rehabilitation
- Abstract
Motor rehabilitation strategies after unilateral stroke suggest that the immobilization of the healthy, unimpaired limb can promote the functional recovery of a paretic limb. In rodents, this has been modeled using casts, harnesses, and other means of restricting the use of the non-paretic forelimb in models of experimental stroke. Here, we evaluated an alternative approach, using botulinum toxin injections to limit the function of the non-paretic forelimb. Adult male rats were subjected to permanent ligation of the left distal middle cerebral artery, resulting in right forelimb paresis. The rats were then subjected to: (1) no treatment; (2) botulinum toxin injections 1 day post stroke; or (3) cast placement 5 days post stroke. Casts were removed after 5 weeks, while the botulinum toxin injection effectively immobilized subjects for approximately the same duration. Rats with bilateral forelimb impairment due to the stroke plus casting or botulinum injections were still able to feed and groom normally. Both immobilization groups showed modest recovery following the stroke compared to those that did not receive immobilization, but the casting approach led to unacceptable levels of animal stress. The botulinum toxin approach to limb immobilization had both advantages and disadvantages over traditional physical limb immobilization. The major advantage was that it was far less stress-inducing to the subject animals and appeared to be well tolerated. A disadvantage was that the paresis took roughly 10 weeks to fully resolve, and any degree of residual paresis could confound the interpretation of the behavioral assessments.
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- 2023
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20. Therapeutic hypothermia for stroke: Unique challenges at the bedside.
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You JS, Kim JY, and Yenari MA
- Abstract
Therapeutic hypothermia has shown promise as a means to improving neurological outcomes at several neurological conditions. At the clinical level, it has been shown to improve outcomes in comatose survivors of cardiac arrest and in neonatal hypoxic ischemic encephalopathy, but has yet to be convincingly demonstrated in stroke. While numerous preclinical studies have shown benefit in stroke models, translating this to the clinical level has proven challenging. Major obstacles include cooling patients with typical stroke who are awake and breathing spontaneously but often have significant comorbidities. Solutions around these problems include selective brain cooling and cooling to lesser depths or avoiding hyperthermia. This review will cover the mechanisms of protection by therapeutic hypothermia, as well as recent progress made in selective brain cooling and the neuroprotective effects of only slightly lowering brain temperature. Therapeutic hypothermia for stroke has been shown to be feasible, but has yet to be definitively proven effective. There is clearly much work to be undertaken in this area., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 You, Kim and Yenari.)
- Published
- 2022
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21. Sexual dimorphism in immune cell responses following stroke.
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Liu J, Sato Y, Falcone-Juengert J, Kurisu K, Shi J, and Yenari MA
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- Disease Susceptibility, Female, Humans, Immunity, Male, Sex Characteristics, Stroke
- Abstract
Recent bodies of work in regard to stroke have revealed significant sex differences in terms of risk and outcome. While differences in sex hormones have been the focus of earlier research, the reasons for these differences are much more complex and require further identification. This review covers differences in sex related immune responses with a focus on differences in immune cell composition and function. While females are more susceptible to immune related diseases, they seem to have better outcomes from stroke at the experimental level with reduced pro-inflammatory responses. However, at the clinical level, the picture is much more complex with worse neurological outcomes from stroke. While the use of exogenous sex steroids can replicate some of these findings, it is apparent that many other factors are involved in the modulation of immune responses. As a result, more research is needed to better understand these differences and identify appropriate interventions and risk modification., (Copyright © 2022. Published by Elsevier Inc.)
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- 2022
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22. Cerebral small vessel disease alters neurovascular unit regulation of microcirculation integrity involved in vascular cognitive impairment.
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Yang Q, Wei X, Deng B, Chang Z, Jin D, Huang Y, Zhang JH, Yenari MA, Jin K, and Wang Q
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- Humans, Microcirculation, Neuroimaging, Cerebral Small Vessel Diseases pathology, Cognitive Dysfunction etiology, Glymphatic System pathology
- Abstract
Cerebral small vessel disease (CSVD) is a generic term used for intracranial vascular disorders caused by the structural changes of cerebral microvessels, including the small arteries, arterioles, capillaries and venules. CSVD exhibits various neuroimaging features and is associated clinical characteristics. Although CSVD is recognized as the leading cause of vascular cognitive impairment (VCI), the underlying mechanism(s) remains elusive. Growing evidence indicates a significant association between altered neurovascular unit (NVU) functioning and the pathophysiology of evolving CSVD-induced VCI. Therefore, research is required to understand how NVU dysregulation contributes to cognitive impairment due to CSVD. In this review, we describe the link between the neuroimaging focal lesions and cognitive alterations. We also discuss the potential pathological role of NVU dysregulation in the entry of pathogens from the blood into the parenchyma by altering the blood-brain barrier (BBB), affecting the cerebral microvascular and consequently cause VCI. Next, we review the coupling of neural activity with cerebral blood flow to control the microvascular perfusion; and the disrupted clearance of metabolic byproducts with CSF-ISF exchange via perivascular pathways and glymphatic system. Finally, we discussed the possible therapeutic interventions in CSVD., (Copyright © 2021. Published by Elsevier Inc.)
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- 2022
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23. Multimodal analysis of gene expression from postmortem brains and blood identifies synaptic vesicle trafficking genes to be associated with Parkinson's disease.
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Gao X, Huang Z, Feng C, Guan C, Li R, Xie H, Chen J, Li M, Que R, Deng B, Cao P, Li M, Lu J, Huang Y, Li M, Yang W, Yang X, Wen C, Liang X, Yang Q, Chao YX, Chan LL, Yenari MA, Jin K, Chaudhuri KR, Zhang J, Tan EK, and Wang Q
- Subjects
- Autopsy, Biomarkers metabolism, Female, Humans, Male, Gene Expression Profiling, Gene Expression Regulation, Gene Regulatory Networks, Nerve Tissue Proteins biosynthesis, Nerve Tissue Proteins genetics, Parkinson Disease genetics, Parkinson Disease metabolism, Protein Interaction Maps, Synaptic Vesicles genetics, Synaptic Vesicles metabolism
- Abstract
Objective: We aimed to identify key susceptibility gene targets in multiple datasets generated from postmortem brains and blood of Parkinson's disease (PD) patients and healthy controls (HC)., Methods: We performed a multitiered analysis to integrate the gene expression data using multiple-gene chips from 244 human postmortem tissues. We identified hub node genes in the highly PD-related consensus module by constructing protein-protein interaction (PPI) networks. Next, we validated the top four interacting genes in 238 subjects (90 sporadic PD, 125 HC and 23 Parkinson's Plus Syndrome (PPS)). Utilizing multinomial logistic regression analysis (MLRA) and receiver operating characteristic (ROC), we analyzed the risk factors and diagnostic power for discriminating PD from HC and PPS., Results: We identified 1333 genes that were significantly different between PD and HCs based on seven microarray datasets. The identified MEturquoise module is related to synaptic vesicle trafficking (SVT) dysfunction in PD (P < 0.05), and PPI analysis revealed that SVT genes PPP2CA, SYNJ1, NSF and PPP3CB were the top four hub node genes in MEturquoise (P < 0.001). The levels of these four genes in PD postmortem brains were lower than those in HC brains. We found lower blood levels of PPP2CA, SYNJ1 and NSF in PD compared with HC, and lower SYNJ1 in PD compared with PPS (P < 0.05). SYNJ1, negatively correlated to PD severity, displayed an excellent power to discriminating PD from HC and PPS., Conclusions: This study highlights that SVT genes, especially SYNJ1, may be promising markers in discriminating PD from HCs and PPS., (© The Author(s) 2020. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.)
- Published
- 2021
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24. Fibrinogen is an Independent Risk Factor for White Matter Hyperintensities in CADASIL but not in Sporadic Cerebral Small Vessel Disease Patients.
- Author
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Guo X, Deng B, Zhong L, Xie F, Qiu Q, Wei X, Wang W, Xu J, Liu G, Hon WPT, Yenari MA, Zhu S, and Wang Q
- Abstract
The relationship between fibrinogen and white matter hyperintensities (WMHs) are inconsistent. Whether there are different relationships between WMHs and fibrinogen in disparate subtypes of cerebral small vessel disease (CSVD) remains unknown. Here, we investigated the roles of plasma fibrinogen in sporadic CSVD (sCSVD) and Cerebral Autosomal Dominant Arteriopathy with Subcortical Infarcts and Leukoencephalopathy (CADASIL) patients. We performed a cross-sectional study that included 74 CSVD patients (19 CADASIL and 55 sporadic) and 74 age- and gender-matched healthy controls (HCs). Plasma fibrinogen was determined, and the severity of WMHs in CSVD patients was rated according to Fazekas scales. Univariate analysis and ordinal logistic regression were performed to evaluate the relationship between fibrinogen and the severity of WMHs in CSVD. Both CADASIL and sCSVD patients showed significantly higher plasma fibrinogen levels than HCs. No significant difference in the plasma fibrinogen level was observed between CADASIL and sCSVD. Univariate analysis and ordinal logistic regression indicated that fibrinogen is an independent risk factor for the severity of WMHs in CADASIL patients (odds ratio [OR] =1.064; 95% Confidence interval (CI, 1.004-1.127); p =0.037). However, age (odds ratio [OR] =1.093; 95% CI (1.033-1.156); P = 0.002), but not fibrinogen (odds ratio [OR] =1.004; 95% CI (0.997-1.011); P=0.262), is an independent risk factor for the severity of WMHs in sCSVD patients. Our results suggest that high levels of plasma fibrinogen are associated with the severity of WMHs in CADASIL but not in sCSVD patients, indicating that the role of fibrinogen may be different in disparate subtypes of CSVD. A better understanding of fibrinogen may yield insights into the pathogenesis of CSVD., Competing Interests: Conflict of interest These authors declare no conflict of interest, (copyright: © 2021 Guo et al.)
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- 2021
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25. Clinical perspectives on ischemic stroke.
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Mizuma A and Yenari MA
- Subjects
- Humans, Ischemic Stroke
- Abstract
Treatments for acute stroke have improved over the past years, but have largely been limited to revascularization strategies. The topic of neuroprotection, or strategies to limit brain tissue damage or even reverse it, has remained elusive. Thus, the clinical mainstays for stroke management have focused on prevention. The lack of clinical translation of neuroprotective therapies which have shown promise in the laboratory may, in part, be due to a historic inattention to comorbidities suffered by a majority of stroke patients. With the advent of more stroke models that include one or more relevant comorbidities, it may be possible to identify effective treatments that may translate into new treatments at the clinical level. In the meantime, we review comorbidities in stroke patients, modification of stroke risk factors and available acute stroke treatments in the clinic., (Published by Elsevier Inc.)
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- 2021
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26. Cystatin C is a potential predictor of unfavorable outcomes for cerebral ischemia with intravenous tissue plasminogen activator treatment: A multicenter prospective nested case-control study.
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Chang Z, Zou H, Xie Z, Deng B, Que R, Huang Z, Weng G, Wu Z, Pan Y, Wang Y, Li M, Xie H, Zhu S, Xiong L, Ct Mok V, Jin K, Yenari MA, Wei X, and Wang Q
- Subjects
- Case-Control Studies, Cystatin C, Fibrinolytic Agents therapeutic use, Humans, Prospective Studies, Retrospective Studies, Thrombolytic Therapy, Tissue Plasminogen Activator therapeutic use, Treatment Outcome, Brain Ischemia drug therapy, Stroke drug therapy
- Abstract
Background and Purpose: The aim of this study was to explore whether cystatin C (CysC) could be used as a potential predictor of clinical outcomes in acute ischemic stroke (AIS) patients treated with intravenous tissue plasminogen activator (IV-tPA)., Methods: We performed an observational study including a retrospective analysis of data from 125 AIS patients with intravenous thrombolysis. General linear models were applied to compare CysC levels between groups with different outcomes; logistic regression analysis and receiver-operating characteristic curves were adopted to identify the association between CysC and the therapeutic effects., Results: Compared with the "good and sustained benefit" (GSB) outcome group (defined as ≥4-point reduction in National Institutes of Health Stroke Scale or a score of 0-1 at 24 h and 7 days) and the "good functional outcome" (GFO) group (modified Rankin Scale score 0-2 at 90 days), serum CysC baseline levels were increased in the non-GSB and non-GFO groups. Logistic regression analysis found that CysC was an independent negative prognostic factor for GSB (odds ratio [OR] 0.010; p = 0.005) and GFO (OR 0.011; p = 0.021) after adjustment for potential influencing factors. Receiver-operating characteristic curves showed the CysC-involved combined models provided credible efficacy for predicting post-90-day favorable clinical outcome (area under the curve 0.86; p < 0.001)., Conclusions: Elevated serum CysC is independently associated with unfavorable clinical outcomes after IV-tPA therapy in AIS. Our findings provide new insights into discovering potential mediators for neuropathological process or treatment in stroke., (© 2020 European Academy of Neurology.)
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- 2021
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27. Microglia, the brain's double agent.
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Yenari MA
- Subjects
- Humans, Brain anatomy & histology, Microglia physiology
- Published
- 2020
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28. Heat Shock Protein 70 (HSP70) Induction: Chaperonotherapy for Neuroprotection after Brain Injury.
- Author
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Kim JY, Barua S, Huang MY, Park J, Yenari MA, and Lee JE
- Subjects
- Adenine analogs & derivatives, Adenine pharmacology, Animals, Benzoquinones pharmacology, Brain metabolism, Brain pathology, Brain Injuries, Traumatic genetics, Brain Injuries, Traumatic metabolism, Brain Injuries, Traumatic pathology, Cell Death drug effects, Disease Models, Animal, Gene Expression Regulation, HSP70 Heat-Shock Proteins agonists, HSP70 Heat-Shock Proteins metabolism, HSP90 Heat-Shock Proteins antagonists & inhibitors, HSP90 Heat-Shock Proteins metabolism, Humans, Lactams, Macrocyclic pharmacology, Neurons metabolism, Neurons pathology, Protein Aggregates drug effects, Protein Folding drug effects, Pyridines pharmacology, Brain drug effects, Brain Injuries, Traumatic drug therapy, HSP70 Heat-Shock Proteins genetics, HSP90 Heat-Shock Proteins genetics, Neurons drug effects, Neuroprotective Agents pharmacology
- Abstract
The 70 kDa heat shock protein (HSP70) is a stress-inducible protein that has been shown to protect the brain from various nervous system injuries. It allows cells to withstand potentially lethal insults through its chaperone functions. Its chaperone properties can assist in protein folding and prevent protein aggregation following several of these insults. Although its neuroprotective properties have been largely attributed to its chaperone functions, HSP70 may interact directly with proteins involved in cell death and inflammatory pathways following injury. Through the use of mutant animal models, gene transfer, or heat stress, a number of studies have now reported positive outcomes of HSP70 induction. However, these approaches are not practical for clinical translation. Thus, pharmaceutical compounds that can induce HSP70, mostly by inhibiting HSP90, have been investigated as potential therapies to mitigate neurological disease and lead to neuroprotection. This review summarizes the neuroprotective mechanisms of HSP70 and discusses potential ways in which this endogenous therapeutic molecule could be practically induced by pharmacological means to ultimately improve neurological outcomes in acute neurological disease.
- Published
- 2020
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29. Vascular, inflammatory and metabolic risk factors in relation to dementia in Parkinson's disease patients with type 2 diabetes mellitus.
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Wang T, Yuan F, Chen Z, Zhu S, Chang Z, Yang W, Deng B, Que R, Cao P, Chao Y, Chan L, Pan Y, Wang Y, Xu L, Lyu Q, Chan P, Yenari MA, Tan EK, and Wang Q
- Subjects
- Age Factors, Aged, Biomarkers blood, Blood Glucose analysis, China epidemiology, Cholesterol, LDL blood, Dementia blood, Dementia diagnosis, Diabetes Mellitus, Type 2 blood, Diabetes Mellitus, Type 2 diagnosis, Female, Fibrinogen analysis, Homocysteine analysis, Humans, Hyperlipidemias blood, Hyperlipidemias diagnosis, Inflammation blood, Inflammation diagnosis, Male, Middle Aged, Parkinson Disease blood, Parkinson Disease diagnosis, Prognosis, Retrospective Studies, Risk Assessment, Risk Factors, Vascular Diseases blood, Vascular Diseases diagnosis, Dementia epidemiology, Diabetes Mellitus, Type 2 epidemiology, Hyperlipidemias epidemiology, Inflammation epidemiology, Parkinson Disease epidemiology, Vascular Diseases epidemiology
- Abstract
There are limited data on vascular, inflammatory, metabolic risk factors of dementia in Parkinson's disease (PD) with type 2 diabetes mellitus (DM) (PD-DM). In a study of 928 subjects comprising of 215 PD with DM (including 31 PD-DM with dementia, PD-DMD), 341 PD without DM (including 31 PD with dementia, PDD) and 372 DM without PD (including 35 DM with dementia, DMD) patients, we investigated if vascular, inflammatory, metabolic, and magnetic resonance imaging (MRI) markers were associated with dementia in PD-DM. Lower fasting blood glucose (FBG<5mmol/L, OR=4.380; 95%CI: 1.748-10.975; p=0.002), higher homocysteine (HCY>15 μ mol/L, OR=3.131; 95%CI: 1.243-7.888; p=0.015) and hyperlipidemia (OR=3.075; 95%CI: 1.142-8.277; p=0.026), increased age (OR=1.043; 95%CI: 1.003-1.084; p=0.034) were the most significant risk factors in PDD patients. Lower low-density lipoprotein cholesterol (LDL-C<2mmol/L, OR=4.499; 95%CI: 1.568-12.909; p=0.005) and higher fibrinogen (>4g/L, OR=4.066; 95%CI: 1.467-11.274; p=0.007) were the most significant risk factors in PD-DMD patients. The area under the curve (AUC) for fibrinogen and LDL-C was 0.717 (P=0.001), with a sensitivity of 80.0% for the prediction of PD-DMD.In summary, we identified several factors including LDL-C and fibrinogen as significant risk factors for PD-DMD and these may have prognostic and treatment implications.
- Published
- 2020
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30. Heat shock protein signaling in brain ischemia and injury.
- Author
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Kim JY, Kim JW, and Yenari MA
- Subjects
- Animals, Humans, Apoptosis physiology, Brain Injuries physiopathology, Brain Ischemia physiopathology, HSP70 Heat-Shock Proteins physiology, Neuroprotection physiology
- Abstract
Heat shock proteins (HSPs) are chaperones that catalyze the refolding of denatured proteins. In addition to their ability to prevent protein denaturation and aggregation, the HSPs have also been shown to modulate many signaling pathways. Among HSPs, the inducible 70 kDa HSP (HSP70) has especially been shown to improve neurological outcome in experimental models of brain ischemia and injury. HSP70 can modulate various aspects of the programmed cell death pathways and inflammation. This review will focus on potential mechanisms of the neuroprotective effects of HSP70 in stroke and brain trauma models. We also comment on potential ways in which HSP70 could be translated into clinical therapies., (Published by Elsevier B.V.)
- Published
- 2020
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31. Cofilin-actin rod formation in experimental stroke is attenuated by therapeutic hypothermia and overexpression of the inducible 70 kD inducible heat shock protein (Hsp70).
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Kurisu K, You J, Zheng Z, Won SJ, Swanson RA, and Yenari MA
- Abstract
Background and Purpose: Cofilin-actin rods are covalently linked aggregates of cofilin-1 and actin. Under ischemic conditions, these rods have been observed in neuronal dendrites and axons and may contribute to the loss of these processes. Hypothermia (Hypo) and the 70 kD inducible heat shock protein (Hsp70) are both known to improve outcomes after stroke, but the mechanisms are uncertain. Here, we evaluated the effect of these factors on cofilin-actin rod formation in a mouse model of stroke., Materials and Methods: Mice were subjected to distal middle cerebral artery occlusion (dMCAO) and treated with Hypo using a paradigm previously shown to be neuroprotective. We similarly studied mice that overexpressed transgenic (Tg) or were deficient knockout (Ko) in the inducible 70 kDa heat shock protein (Hsp70), also previously shown to be protective by our group and others. Cofilin-actin rod formation was assessed by histological analysis at 4 and 24 h after dMCAO. Its expression was analyzed in three different regions, namely, infarct core (the center of the infarct), middle cerebral artery (MCA) borderzone (the edge of the brain regions supplied by the MCA), and the ischemic borderzone (border of ischemic lesion). Ischemic lesion size and neurological deficits were also assessed., Results: Both Hypo-treated and Hsp70 Tg mice had smaller lesion sizes and improved neurological outcomes, whereas Hsp70 Ko mice had larger lesion sizes and worsened neurological outcomes. Cofilin-actin rods were increased after stroke, but were reduced by therapeutic Hypo and in Hsp70 Tg mice. In contrast, cofilin-actin rods were increased in ischemic brains of Hsp70 Ko mice., Conclusions: Cofilin-actin rod formation was suppressed under the conditions of neuroprotection and increased under circumstances where outcome was worsened. This suggests that cofilin-actin rods may act to participate in or exacerbate ischemic pathology and warrants further study as a potential therapeutic target., Competing Interests: There are no conflicts of interest., (Copyright: © 2019 Brain Circulation.)
- Published
- 2019
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32. Models of poststroke depression and assessments of core depressive symptoms in rodents: How to choose?
- Author
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Tao X, Yang W, Zhu S, Que R, Liu C, Fan T, Wang J, Mo D, Zhang Z, Tan J, Jin K, Yenari MA, Song T, and Wang Q
- Subjects
- Animals, Rodentia, Depression etiology, Disease Models, Animal, Stroke complications, Stroke psychology
- Abstract
Our previous studies have indicated that depression and declined cognition have been involved in some neurodegenerative diseases including Stroke, Parkinson's diseases and Vascular Parkinsonism. Post-stroke depression (PSD) is the most common psychiatric disorder following a stroke and has high morbidity and mortality. Studies on PSD are increasingly common, but the specific mechanisms remain unknown. Current research mainly includes clinical and animal aspects. Questionnaires and peripheral blood examination are two of the most common methods used to study clinical PSD. The results of questionnaires are influenced by multiple factors such as disease history, education background, occupation, economic status, family relationships and social support. There are certain limitations to blood sample testing; for example, it is influenced by cerebrovascular diseases and some other disruptions of the internal environment. It is difficult for either method to fully clarify the pathophysiological mechanism of PSD. Animal models provide alternative methods to further understand the pathophysiological mechanisms of PSD, such as the involvement of neuronal circuits and cytokines. More than ten animal models of PSD have been developed, and new models are constantly being introduced. Therefore, it is important to choose the appropriate model for any given study. In this paper, we will discuss the characteristics of the different models of PSD and comment on the advantages and disadvantages of each model, drawing from research on model innovation. Finally, we briefly describe the current assessment methods for the core symptoms of PSD models, point out the shortcomings, and present the improved sucrose preference test as a rational evaluation of anhedonia., (Copyright © 2019. Published by Elsevier Inc.)
- Published
- 2019
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33. Triggering receptor expressed on myeloid cells-2 expression in the brain is required for maximal phagocytic activity and improved neurological outcomes following experimental stroke.
- Author
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Kurisu K, Zheng Z, Kim JY, Shi J, Kanoke A, Liu J, Hsieh CL, and Yenari MA
- Subjects
- Animals, Brain pathology, Male, Membrane Glycoproteins genetics, Mice, Mice, Inbred C57BL, Mice, Knockout, Microglia immunology, Microglia pathology, Myeloid Cells immunology, Myeloid Cells pathology, Phagocytes immunology, Phagocytes pathology, Receptors, Immunologic genetics, Stroke genetics, Stroke pathology, Brain immunology, Membrane Glycoproteins immunology, Phagocytosis, Receptors, Immunologic immunology, Stroke immunology
- Abstract
Triggering receptor expressed on myeloid cells-2 (TREM2) is an innate immune receptor that promotes phagocytosis by myeloid cells such as microglia and macrophages. We previously showed that TREM2 deficiency worsened outcomes from experimental stroke and impeded phagocytosis. However, myeloid cells participating in stroke pathology include both brain resident microglia and circulating macrophages. We now clarify whether TREM2 on brain microglia or circulating macrophages contribute to its beneficial role in ischemic stroke by generating bone marrow (BM) chimeric mice. BM chimera mice from TREM2 knockout (KO) or wild type (Wt) mice were used as donor and recipient mice. Mice were subjected to experimental stroke, and neurological function and infarct volume were assessed. Mice with intact TREM2 in brain microglia showed better neurological recovery and reduced infarct volumes, compared with mice lacking microglial TREM2. Myeloid cell activation and numbers of phagocytes were decreased in mice lacking brain TREM2, compared with mice with intact brain TREM2. These results suggest that TREM2 expression is important for post-stroke recovery, and that TREM2 expression on brain resident microglia is more essential to this recovery, than that of circulating macrophages. These findings might suggest a new therapeutic target for cerebrovascular diseases.
- Published
- 2019
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34. Plasma Lipoprotein-associated Phospholipase A2 and Superoxide Dismutase are Independent Predicators of Cognitive Impairment in Cerebral Small Vessel Disease Patients: Diagnosis and Assessment.
- Author
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Zhu S, Wei X, Yang X, Huang Z, Chang Z, Xie F, Yang Q, Ding C, Xiang W, Yang H, Xia Y, Feng ZP, Sun HS, Yenari MA, Shi L, Mok VC, and Wang Q
- Abstract
Lipoprotein-associated phospholipase A2 (Lp-PLA2) and superoxide dismutase (SOD) are linked to regulating vascular/neuro-inflammation and stroke. Using a retrospective design, we investigated whether circulating Lp-PLA2 and SOD in cerebral small vessel disease (CSVD) patients were associated with cognitive impairment. Eighty-seven CSVD patients were recruited. Plasma Lp-PLA2 and SOD were determined, and cognitive status was measured by the Mini-Mental State Examination (MMSE) and Montreal Cognitive Assessment (MoCA). The severity of white matter hypoerintensities (WMHs) in CSVD patients was rated according to Fazekas scales, and Lp-PLA2/SOD levels and MMSE/MoCA were compared. Multiple linear regressions were used to evaluate the relationship between Lp-PLA2 and SOD and the cognitive impairment. Ordinal logistic regression and generalized linear models (OLRGLMs) were applied to confirm whether Lp-PLA2 and SOD are independent risk factors for cognitive impairment in CVSD. Lp-PLA2 and SOD with mild or severe cognitive impairment were lower than those with normal congnition. Lp-PLA2 and SOD in CSVD patients with severe WMHs were significantly lower than those with mild or moderate WMH lesions. We noted positive linear associations of Lp-PLA and SOD with cognitive impairment in CSVD, independent of LDL-C. OLRGLMs confirmed that Lp-PLA2 and SOD were independent risk factors of cognitive impairment in CSVD. Lp-PLA2 and SOD are independently associated with cognitive impairment and WMH lesion, and may be useful for the rapid evaluation of cognitive impairment in CSVD. Lp-PLA2/SOD are modifiable factors that may be considered as therapeutic targets for preventing cognitive impairment in CSVD., Competing Interests: Potential Conflicts of Interest The authors declare no conflict of interest.
- Published
- 2019
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35. The role of NOX inhibitors in neurodegenerative diseases.
- Author
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Barua S, Kim JY, Yenari MA, and Lee JE
- Abstract
Oxidative stress is a key player in both chronic and acute brain disease due to the higher metabolic demand of the brain. Among the producers of free radicals, NADPH-oxidase (NOX) is a major contributor to oxidative stress in neurological disorders. In the brain, the superoxide produced by NOX is mainly found in leukocytes. However, recent studies have reported that it can be found in several other cell types. NOX has been reported to regulate neuronal signaling, memory processing, and central cardiovascular homeostasis. However, overproduction of NOX can contribute to neurotoxicity, CNS degeneration, and cardiovascular disorders. Regarding the above functions, NOX has been shown to play a crucial role in chronic CNS diseases like Parkinson's disease (PD), Alzheimer's disease (AD), Huntington's disease (HD), multiple sclerosis (MS), and amyotrophic lateral sclerosis (ALS), and in acute CNS disorders such as stroke, spinal cord injury, traumatic brain injury (TBI), and related cerebrovascular diseases. NOX is a multi-subunit complex consisting of two membrane-associated and four cytosolic subunits. Thus, in recent years, inhibition of NOX activity has drawn a great deal of attention from researchers in the field of treating chronic and acute CNS disorders and preventing secondary complications. Mounting evidence has shown that NOX inhibition is neuroprotective and that inhibiting NOX in circulating immune cells can improve neurological disease conditions. This review summarizes recent studies on the therapeutic effects and pharmacological strategies regarding NOX inhibitors in chronic and acute brain diseases and focuses on the hurdles that should be overcome before their clinical implementation.
- Published
- 2019
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36. Microglial Calcium Release-Activated Calcium Channel Inhibition Improves Outcome from Experimental Traumatic Brain Injury and Microglia-Induced Neuronal Death.
- Author
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Mizuma A, Kim JY, Kacimi R, Stauderman K, Dunn M, Hebbar S, and Yenari MA
- Subjects
- Animals, Brain metabolism, Calcium Signaling physiology, Cell Line, Inflammation metabolism, Interferon-gamma pharmacology, Male, Mice, Microglia metabolism, NF-kappa B metabolism, Nitric Oxide metabolism, ORAI1 Protein metabolism, Stromal Interaction Molecule 1 metabolism, Brain drug effects, Brain Injuries, Traumatic metabolism, Calcium metabolism, Calcium Channel Blockers pharmacology, Calcium Release Activated Calcium Channels metabolism, Microglia drug effects
- Abstract
Store-operated Ca
2+ entry (SOCE) mediated by calcium release-activated calcium (CRAC) channels contributes to calcium signaling. The resulting intracellular calcium increases activate calcineurin, which in turn activates immune transcription factor nuclear factor of activated T cells (NFAT). Microglia contain CRAC channels, but little is known whether these channels play a role in acute brain insults. We studied a novel CRAC channel inhibitor to explore the therapeutic potential of this compound in microglia-mediated injury. Cultured microglial BV2 cells were activated by Toll-like receptor agonists or IFNγ. Some cultures were treated with a novel CRAC channel inhibitor (CM-EX-137). Western blots revealed the presence of CRAC channel proteins STIM1 and Orai1 in BV2 cells. CM-EX-137 decreased nitric oxide (NO) release and inducible nitric oxide synthase (iNOS) expression in activated microglia and reduced agonist-induced intracellular calcium accumulation in microglia, while suppressing inflammatory transcription factors nuclear factor kappa B (NF-κB) and nuclear factor of activated T cells (NFAT). Male C57/BL6 mice exposed to experimental brain trauma and treated with CM-EX-137 had decreased lesion size, brain hemorrhage, and improved neurological deficits with decreased microglial activation, iNOS and Orai1 and STIM1 levels. We suggest a novel anti-inflammatory approach for managing acute brain injury. Our observations also shed light on new calcium signaling pathways not described previously in brain injury models.- Published
- 2019
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37. Therapeutic Hypothermia and Neuroprotection in Acute Neurological Disease.
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Kurisu K, Kim JY, You J, and Yenari MA
- Subjects
- Acute Disease, Animals, Brain physiopathology, Humans, Nervous System Diseases physiopathology, Hypothermia, Induced, Nervous System Diseases therapy, Neuroprotection
- Abstract
Therapeutic hypothermia has consistently been shown to be a robust neuroprotectant in many labs studying different models of neurological disease. Although this therapy has shown great promise, there are still challenges at the clinical level that limit the ability to apply this routinely to each pathological condition. In order to overcome issues involved in hypothermia therapy, understanding of this attractive therapy is needed. We review methodological concerns surrounding therapeutic hypothermia, introduce the current status of therapeutic cooling in various acute brain insults, and review the literature surrounding the many underlying molecular mechanisms of hypothermic neuroprotection. Because recent work has shown that body temperature can be safely lowered using pharmacological approaches, this method may be an especially attractive option for many clinical applications. Since hypothermia can affect multiple aspects of brain pathophysiology, therapeutic hypothermia could also be considered a neuroprotection model in basic research, which would be used to identify potential therapeutic targets. We discuss how research in this area carries the potential to improve outcome from various acute neurological disorders., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)
- Published
- 2019
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38. Targeting Reperfusion Injury in the Age of Mechanical Thrombectomy.
- Author
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Mizuma A, You JS, and Yenari MA
- Subjects
- Humans, Reperfusion Injury etiology, Thrombectomy adverse effects, Treatment Outcome, Brain Ischemia surgery, Reperfusion Injury prevention & control, Thrombectomy methods
- Published
- 2018
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39. Therapeutic hypothermia for ischemic stroke; pathophysiology and future promise.
- Author
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Kurisu K and Yenari MA
- Subjects
- Animals, Humans, Hypothermia, Induced trends, Brain Ischemia complications, Hypothermia, Induced methods, Stroke etiology, Stroke therapy
- Abstract
Therapeutic hypothermia, or cooling of the body or brain for the purposes of preserving organ viability, is one of the most robust neuroprotectants at both the preclinical and clinical levels. Although therapeutic hypothermia has been shown to improve outcome from related clinical conditions, the significance in ischemic stroke is still under investigation. Numerous pre-clinical studies of therapeutic hypothermia has suggested optimal cooling conditions, such as depth, duration, and temporal therapeutic window for effective neuroprotection. Several studies have also explored mechanisms underlying the mechanisms of neuroprotection by therapeutic hypothermia. As such, it appears that cooling affects multiple aspects of brain pathophysiology, and regulates almost every pathway involved in the evolution of ischemic stroke. This multifaceted mechanism is thought to contribute to its strong neuroprotective effect. In order to carry out this therapy in optimal clinical settings, methodological and pathophysiological understanding is crucial. However, more investigation is still needed to better understand the underlying mechanisms of this intervention, and to overcome clinical barriers which seem to preclude the routine use therapeutic hypothermia in stroke. This article is part of the Special Issue entitled 'Cerebral Ischemia'., (Published by Elsevier Ltd.)
- Published
- 2018
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40. Advances in Stroke 2017.
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Bernhardt J, Zorowitz RD, Becker KJ, Keller E, Saposnik G, Strbian D, Dichgans M, Woo D, Reeves M, Thrift A, Kidwell CS, Olivot JM, Goyal M, Pierot L, Bennett DA, Howard G, Ford GA, Goldstein LB, Planas AM, Yenari MA, Greenberg SM, Pantoni L, Amin-Hanjani S, and Tymianski M
- Subjects
- Antibodies, Monoclonal, Humanized therapeutic use, Antihypertensive Agents therapeutic use, Arginine analogs & derivatives, Arginine therapeutic use, Delivery of Health Care, Factor Xa therapeutic use, Health Policy, Humans, Hypertension drug therapy, Outcome Assessment, Health Care, Piperazines therapeutic use, Recombinant Proteins therapeutic use, Stroke diagnostic imaging, Stroke genetics, Stroke prevention & control, Thrombectomy, Thrombolytic Therapy, Anticoagulants adverse effects, Antidotes therapeutic use, Intracranial Hemorrhages chemically induced, Stroke therapy, Stroke Rehabilitation
- Published
- 2018
- Full Text
- View/download PDF
41. The 70-kDa heat shock protein (Hsp70) as a therapeutic target for stroke.
- Author
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Kim JY, Han Y, Lee JE, and Yenari MA
- Subjects
- Animals, Brain Ischemia physiopathology, Cell Death drug effects, Drug Design, HSP70 Heat-Shock Proteins drug effects, HSP70 Heat-Shock Proteins metabolism, HSP90 Heat-Shock Proteins antagonists & inhibitors, HSP90 Heat-Shock Proteins metabolism, Humans, Molecular Targeted Therapy, Protein Folding, Stroke physiopathology, Brain Ischemia drug therapy, Neuroprotective Agents pharmacology, Stroke drug therapy
- Abstract
Introduction: The 70-kDa heat shock protein (Hsp70) is a cytosolic chaperone which facilitates protein folding, degradation, complex assembly, and translocation. Following stroke, these functions have the potential to lead to cytoprotection, and this has been demonstrated using genetic mutant models, direct gene transfer or the induction of Hsp70 via heat stress, approaches which limit its translational utility. Recently, the investigation of Hsp70-inducing pharmacological compounds, which, through their ability to inhibit Hsp90, has obvious clinical implications in terms of potential therapies to mitigate cell death and inflammation, and lead to neuroprotection from brain injury. Areas covered: In this review, we will focus on the role of Hsp70 in cell death and inflammation, and the current literature surrounding the pharmacological induction in acute ischemic stroke models with comments on potential applications at the clinical level. Expert opinion: Such neuroprotectants could be used to synergistically improve neurological outcome or to extend the time window of existing interventions, thus increasing the numbers of stroke victims eligible for treatment.
- Published
- 2018
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42. Reconsidering Neuroprotection in the Reperfusion Era.
- Author
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Savitz SI, Baron JC, Yenari MA, Sanossian N, and Fisher M
- Subjects
- Animals, Brain Ischemia prevention & control, Humans, Reperfusion methods, Reperfusion Injury prevention & control, Thrombectomy methods, Brain Ischemia drug therapy, Neuroprotection drug effects, Neuroprotective Agents therapeutic use, Reperfusion Injury drug therapy
- Published
- 2017
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43. Anti-Inflammatory Targets for the Treatment of Reperfusion Injury in Stroke.
- Author
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Mizuma A and Yenari MA
- Abstract
While the mainstay of acute stroke treatment includes revascularization via recombinant tissue plasminogen activator or mechanical thrombectomy, only a minority of stroke patients are eligible for treatment, as delayed treatment can lead to worsened outcome. This worsened outcome at the experimental level has been attributed to an entity known as reperfusion injury (R/I). R/I is occurred when revascularization is delayed after critical brain and vascular injury has occurred, so that when oxygenated blood is restored, ischemic damage is increased, rather than decreased. R/I can increase lesion size and also worsen blood barrier breakdown and lead to brain edema and hemorrhage. A major mechanism underlying R/I is that of poststroke inflammation. The poststroke immune response consists of the aberrant activation of glial cell, infiltration of peripheral leukocytes, and the release of damage-associated molecular pattern (DAMP) molecules elaborated by ischemic cells of the brain. Inflammatory mediators involved in this response include cytokines, chemokines, adhesion molecules, and several immune molecule effectors such as matrix metalloproteinases-9, inducible nitric oxide synthase, nitric oxide, and reactive oxygen species. Several experimental studies over the years have characterized these molecules and have shown that their inhibition improves neurological outcome. Yet, numerous clinical studies failed to demonstrate any positive outcomes in stroke patients. However, many of these clinical trials were carried out before the routine use of revascularization therapies. In this review, we cover mechanisms of inflammation involved in R/I, therapeutic targets, and relevant experimental and clinical studies, which might stimulate renewed interest in designing clinical trials to specifically target R/I. We propose that by targeting anti-inflammatory targets in R/I as a combined therapy, it may be possible to further improve outcomes from pharmacological thrombolysis or mechanical thrombectomy.
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- 2017
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44. Hypothermia Identifies Dynamin as a Potential Therapeutic Target in Experimental Stroke.
- Author
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Kim JY, Kim N, Lee JE, and Yenari MA
- Subjects
- Animals, Apoptosis, Brain metabolism, Brain pathology, Brain Ischemia metabolism, Disease Models, Animal, Fas Ligand Protein metabolism, Male, Mice, Mice, Inbred C57BL, Random Allocation, Dynamins metabolism, Hypothermia, Induced, Stroke metabolism
- Abstract
Apoptosis is a cell death pathway that is activated in ischemic stroke. The interaction between Fas and its ligand (FasL) initiates a complex pattern of intracellular events involving the recruitment of specific adaptor proteins and the development of apoptosis. We recently reported that dynamin is increased after experimental stroke, and its inhibition improves neurological outcome. Dynamin has been shown to transport Fas from the endoplasmic reticulum to the cell surface where it can be bound by its ligand, FasL. Hypothermia has been shown to improve outcome in numerous stroke models, and this protection is associated with reduced apoptosis and Fas expression. To explore the contribution of dynamin to hypothermic neuroprotection, we subjected mice to distal middle cerebral artery occlusion (dMCAO) and applied one of two cooling paradigms: one where cooling began at the onset of dMCAO (early hypothermia) and another where cooling began 1 hour later (delayed hypothermia), compared with normothermia (Norm). Both cooling paradigms reduced numbers of apoptotic cells, as well as Fas and dynamin compared with Norm. Fas and dynamin were co-expressed in neurons. Neuronal cultures were exposed to oxygen glucose deprivation. Hypothermia decreased dynamin as well as surface expression of Fas, and this correlated to reduced cell death. The results of this study suggest that dynamin may participate in the Fas-mediated apoptotic pathway, and its reduction may be linked to hypothermic neuroprotection.
- Published
- 2017
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45. NOX Inhibitors - A Promising Avenue for Ischemic Stroke.
- Author
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Kim JY, Park J, Lee JE, and Yenari MA
- Abstract
NADPH-oxidase (NOX) mediated superoxide originally found on leukocytes, but now recognized in several types of cells in the brain. It has been shown to play an important role in the progression of stroke and related cerebrovascular disease. NOX is a multisubunit complex consisting of 2 membrane-associated and 4 cytosolic subunits. NOX activation occurs when cytosolic subunits translocate to the membrane, leading to transport electrons to oxygen, thus producing superoxide. Superoxide produced by NOX is thought to function in long-term potentiation and intercellular signaling, but excessive production is damaging and has been implicated to play an important role in the progression of ischemic brain. Thus, inhibition of NOX activity may prove to be a promising treatment for ischemic brain as well as an adjunctive agent to prevent its secondary complications. There is mounting evidence that NOX inhibition in the ischemic brain is neuroprotective, and targeting NOX in circulating immune cells will also improve outcome. This review will focus on therapeutic effects of NOX assembly inhibitors in brain ischemia and stroke. However, the lack of specificity and toxicities of existing inhibitors are clear hurdles that will need to be overcome before this class of compounds could be translated clinically.
- Published
- 2017
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46. Activated complement protein C5a does not affect brain-derived endothelial cell viability and zonula occludens-1 levels following oxygen-glucose deprivation.
- Author
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Khoyetsyan A, Kacimi R, Tsakanova G, Boyajyan A, Arakelyan A, and Yenari MA
- Abstract
Background and Purpose: Ischemic brain injury induces both functional and structural disarray affecting the blood-brain barrier (BBB) which in return aggravates stroke outcomes. Complement system and its bioactive proteins are important molecular responders to ischemia. C5a protein along with its receptor C5a receptor 1 is a key component of this system with potent pro-inflammatory and chemoattractant properties. The purpose of this study is to investigate the role of C5a protein and its receptor which are believed to participate in the inflammatory response that follows ischemic insult., Materials and Methods: To mimic an ischemic in vivo event in which C5a may contact brain endothelial cells after injury, we studied oxygen-glucose deprivation (OGD) followed by reperfusion in brain microvascular endothelial cells (b.End. 3) by only added C5a at the time of reperfusion. Cell death and viability were estimated by trypan blue and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays, respectively. Tight junction protein zonula occluden (ZO-1) levels were analyzed by Western blot analysis, and nitric oxide (NO) was assessed using the Griess reagent., Results: Brain-derived endothelial cell was susceptible to OGD-induced injury in a duration-dependent manner as was the presence of ZO-1 protein. However, the addition of C5a protein had no notable effects even when used at high concentrations up to 100 nM. While OGD led to reduction in ZO-1 protein levels, no change was seen following the addition of C5a. Finally, OGD led unexpectedly to small decreases in NO generation, but this was again unaltered by C5a., Conclusions: Our study suggests that complement system protein C5a may not have a direct role in the disruption of BBB, following brain ischemia. This is in contrary with previous literature that suggests a possible role of this protein in the inflammatory response to ischemia., Competing Interests: There are no conflicts of interest.
- Published
- 2017
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47. 70-kDa Heat Shock Protein Downregulates Dynamin in Experimental Stroke: A New Therapeutic Target?
- Author
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Kim JY, Kim N, Zheng Z, Lee JE, and Yenari MA
- Subjects
- Animals, Apoptosis drug effects, Apoptosis physiology, Caspase 8 metabolism, Cell Line, Tumor, Dynamins genetics, HSP70 Heat-Shock Proteins genetics, Hydrazones pharmacology, Infarction, Middle Cerebral Artery pathology, Mice, Mice, Knockout, Mice, Transgenic, Neurons drug effects, Neurons metabolism, Neurons pathology, Stroke pathology, fas Receptor genetics, fas Receptor metabolism, Down-Regulation, Dynamins metabolism, HSP70 Heat-Shock Proteins metabolism, Infarction, Middle Cerebral Artery metabolism, Stroke metabolism
- Abstract
Background and Purpose: The 70-kDa heat shock protein (Hsp70) protects brain cells in models of cerebral ischemia. Proteomic screening of mice subjected to middle cerebral artery occlusion identified dynamin as a major downregulated protein in Hsp70-overexpressing mice (Hsp70 transgenic mice). Dynamin-1 is expressed in neurons and participates in neurotransmission, but also transports the death receptor Fas to the cell surface, where it can be bound by its ligand and lead to apoptosis., Methods: Mice were subjected to distal middle cerebral artery occlusion. Neuro-2a cells were subjected to oxygen glucose deprivation. Hsp70 transgenic and Hsp70-deficient (Hsp70 knockout) mice were compared with wild-type mice for histological and behavioral outcomes. Some mice and neuro-2a cell cultures were given dynasore, a dynamin inhibitor., Results: Hsp70 transgenic mice had better outcomes, whereas Hsp70 knockout mice had worse outcomes compared with wild-type mice. This correlated with decreased and increased dynamin expression, respectively. Dynamin colocalized to neurons and Fas, with higher Fas levels and increased caspase-8 expression. Hsp70 induction in neuro-2a cells was protected from oxygen glucose deprivation, while downregulating dynamin and Fas expression. Further, dynamin inhibition was found to be neuroprotective., Conclusions: Dynamin may facilitate Fas-mediated apoptotic death in the brain, and Hsp70 may protect by preventing this trafficking. Dynamin should be explored as a new therapeutic target for neuroprotection., (© 2016 American Heart Association, Inc.)
- Published
- 2016
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48. Pharmacologic heat shock protein 70 induction confers cytoprotection against inflammation in gliovascular cells.
- Author
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Kacimi R and Yenari MA
- Subjects
- Animals, Brain drug effects, Brain immunology, Cell Line, Cell Survival physiology, Cells, Cultured, Endothelial Cells drug effects, Gene Knockdown Techniques, HSP70 Heat-Shock Proteins genetics, HSP90 Heat-Shock Proteins antagonists & inhibitors, HSP90 Heat-Shock Proteins metabolism, Lipopolysaccharides, Mice, Microglia drug effects, Microvessels drug effects, NF-kappa B metabolism, Nitric Oxide metabolism, Nitric Oxide Synthase Type II metabolism, Endothelial Cells immunology, HSP70 Heat-Shock Proteins metabolism, Microglia immunology, Microvessels immunology
- Abstract
The inhibition of the 90-kDa heat shock protein (HSP90) leads to upregulation of the 70-kDa-inducible HSP70. HSP70 has been previously shown to be neuroprotective and anti-inflammatory. Geldanamycin (GA) and other HSP90 inhibitors have emerged as promising therapeutic agents in cancer, presumably owing to their ability to upregulate HSP70. However, the effects of HSP90 inhibition in brain inflammation are still unclear. We investigate the effect of a panel of HSP90 inhibitors on endotoxin-activated microglia and eventual protection from brain-derived endothelial cells. Prior studies have shown that GA protects brain cells from oxidative stress. We show here that when astrocytes or microglial BV2 cells were pretreated with GA or other HSP90 inhibitors, endotoxin-induced cell death was reduced in cocultures of BV2 microglia and brain-derived endothelial cells (bEND.3). Endotoxin-stimulated BV2 cells led to increased nitric oxide (NO) and inducible nitric oxide synthase which was prevented by treatment with all HSP90 inhibitors. HSP90 inhibitors also prevented lipopolysaccharide (LPS)-induced BV2 cell death. We also found that HSP90 inhibition blocked nuclear translocation of nuclear factor kappa B and attenuated IκBα degradation, and inhibited LPS-activated JAK-STAT phosphorylation. We show that pharmacologic inhibition of HSP90 with subsequent HSP70 induction protects cells that comprise the cerebral vasculature against cell death owing to proinflammatory stimuli. This approach may have therapeutic potential in neurological conditions with an inflammatory component., (© 2015 Wiley Periodicals, Inc.)
- Published
- 2015
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49. The role of the microglia in acute CNS injury.
- Author
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Kawabori M and Yenari MA
- Subjects
- Animals, Humans, Stroke pathology, Central Nervous System injuries, Microglia pathology
- Abstract
Microglia are considered the brain's resident immune cell involved in immune defense, immunocompetence, and phagocytosis. They maintain tissue homeostasis within the brain and spinal cord under normal condition and serves as its initial host defense system. However, when the central nervous system (CNS) faces injury, microglia respond through signaling molecules expressed or released by neighboring cells. Microglial responses are dual in nature. They induce a nonspecific immune response that may exacerbate CNS injury, especially in the acute stages, but are also essential to CNS recovery and repair. The full range of microglial mechanisms have yet to be clarified, but there is accumulating knowledge about microglial activation in acute CNS injury. Microglial responses require hours to days to fully develop, and may present a therapeutic target for intervention with a much longer window of opportunity compare to other neurological treatments. The challenge will be to find ways to selectively suppress the deleterious effects of microglial activation without compromising its beneficial functions. This review aims to provide an overview of the recent progress relating on the deleterious and beneficial effect of microglia in the setting of acute CNS injury and the potential therapeutic intervention against microglial activation to CNS injury.
- Published
- 2015
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50. Triggering receptor expressed on myeloid cells 2 (TREM2) deficiency attenuates phagocytic activities of microglia and exacerbates ischemic damage in experimental stroke.
- Author
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Kawabori M, Kacimi R, Kauppinen T, Calosing C, Kim JY, Hsieh CL, Nakamura MC, and Yenari MA
- Subjects
- Animals, Cell Hypoxia, Cells, Cultured, Foam Cells metabolism, Foam Cells pathology, Infarction, Middle Cerebral Artery pathology, Membrane Glycoproteins deficiency, Membrane Glycoproteins genetics, Mice, Mice, Inbred C57BL, Microglia physiology, Neurons metabolism, Neurons pathology, Receptors, Immunologic deficiency, Receptors, Immunologic genetics, Infarction, Middle Cerebral Artery metabolism, Membrane Glycoproteins metabolism, Microglia metabolism, Phagocytosis, Receptors, Immunologic metabolism
- Abstract
Clearing cellular debris after brain injury represents an important mechanism in regaining tissue homeostasis and promoting functional recovery. Triggering receptor expressed on myeloid cells-2 (TREM2) is a newly identified receptor expressed on microglia and is thought to phagocytose damaged brain cells. The precise role of TREM2 during ischemic stroke has not been fully understood. We explore TREM2 in both in vitro and in vivo stroke models and identify a potential endogenous TREM2 ligand. TREM2 knockdown in microglia reduced microglial activation to an amoeboid phenotype and decreased the phagocytosis of injured neurons. Phagocytosis and infarcted brain tissue resorption was reduced in TREM2 knock-out (KO) mice compared with wild-type (WT) mice. TREM2 KO mice also had worsened neurological recovery and decreased viable brain tissue in the ipsilateral hemisphere. The numbers of activated microglia and phagocytes in TREM2 KO mice were decreased compared with WT mice, and foamy macrophages were nearly absent in the TREM2 KO mice. Postischemia, TREM2 was highly expressed on microglia and TREM2-Fc fusion protein (used as a probe to identify potential TREM2 binding partners) bound to an unknown TREM2 ligand that colocalized to neurons. Oxygen glucose deprivation-exposed neuronal media, or cellular fractions containing nuclei or purified DNA, but not cytosolic fractions, stimulated signaling through TREM2. TREM2-Fc fusion protein pulled down nucleic acids from ischemic brain lysate. These findings establish the relevance of TREM2 in the phagocytosis of the infarcted brain and emphasize its role in influencing neurological outcomes following stroke. Further, nucleic acids may be one potential ligand of TREM2 in brain ischemia., (Copyright © 2015 the authors 0270-6474/15/353384-13$15.00/0.)
- Published
- 2015
- Full Text
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