1. Taenia solium metacestode fasciclin-like protein is reactive with sera of chronic neurocysticercosis.
- Author
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Bae, Young‐An, Yeom, Joon‐Sup, Wang, Hu, Kim, Seon‐Hee, Ahn, Chun‐Seob, Kim, Jin‐Taek, Yang, Hyun‐Jong, and Kong, Yoon
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TAENIA solium , *TAPEWORM infections , *NEUROCYSTICERCOSIS , *CENTRAL nervous system , *BIOMARKERS , *ANTISENSE DNA - Abstract
Objectives Neurocysticercosis ( NC), an infection of the central nervous system with Taenia solium metacestodes ( Ts M), invokes a formidable neurological disease. A bundle of antigens is applicable for serodiagnosis of active cases, while they demonstrate fairly low reactivity against sera of chronic NC. Identification of sensitive biomarkers for chronic NC is critical for appropriate management of patients. Methods Proteome analysis revealed several isoforms of 65- and 83-kDa TsM fasciclin-like proteins ( Ts MFas) to be highly reactive with sera of chronic NC. A c DNA encoding one of the 83-kDa Ts MFas (Ts MFas1) was isolated from a c DNA library. We expressed a recombinant protein (r Ts MFas1) and evaluated its diagnostic potential employing sera from chronic NC ( n = 80), tissue-invasive cestodiases ( n = 169) and trematodiases ( n = 80) and those of normal controls ( n = 50). Results Secretory Ts MFas1 was composed of 766 amino acid polypeptide and harboured fasciclin and fasciclin-superfamily domains. The protein was constitutively expressed in metacestode and adult stages, with preferential locality in the scolex. Bacterially expressed r Ts MFas1 exhibited 78.8% sensitivity (63/80 cases) and 93% specificity (278/299 samples) in diagnosing chronic NC. Some cross-reactivity was observed with sera of cystic echinococcosis (10/56, 17.8%) and sparganosis (4/50, 8%). Positive and negative predictive values were 75% and 95.5%, respectively. Conclusion TsM fasciclin-like protein may be useful for differential diagnosis of chronic NC in clinical settings, especially where both NC and other infectious cerebral granulomatoses are prevalent. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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