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6 results on '"Yesufu HM"'

1. Concurrent Infections with Schistosomiasis Typhoid Fever and Malaria among Patients in Edo State Nigeria

Author

Obi, AA, Yesufu, HM, Fagbenro-Beyioku, AF, and Obi, CL

Subjects
schistosomiasis, malaria, Nigeria, typhoid fever
Abstract

The prevalence of typhoid fever, malaria and schistosomiasis among 268 symptomatic patients in Edo State, Nigeria, was investigated. Blood, urine and stool samples were collected from 268 patients (163 males and 105 females). The results showed that of the total number examined, 65 (39.9%) males and 39 (37.1%) females had schistosome eggs in their urine. Ninety-five (58.3%) males and 67 (63.8%) females were infected with Salmonella typhi, while 88 (54.0%) males and 54 (51.4%) females had malaria parasites. Dual infections of schistosomiasis and typhoid fever were higher in females (17.14%) than males (16.6%). Thirty-three (20.3%) males and 24 (22.86%) females had both schistosomiasis and malaria. Typhoid fever and malaria occurred in 53 (32.5%) and 35 (33.3%) males and females, respectively. Triple infections with typhoid fever, malaria and schistosomiasis occurred among 16 (9.8%) males and 13 (12.4%) females. For all cases, children were more frequently infected than adults. This study provide insight into problems and complications in tropical diseases as dual or triple infections could occur concurrently in a patient.

Published
1996

2. DNA methylase from Pisum sativum.

Author

Yesufu HM, Hanley A, Rinaldi A, and Adams RL

Subjects
Base Sequence, DNA metabolism, DNA-Cytosine Methylases genetics, Electrophoresis, Polyacrylamide Gel, Fabaceae genetics, Fabaceae growth & development, Molecular Weight, Sodium Chloride pharmacology, Solubility, Substrate Specificity, Time Factors, DNA-Cytosine Methylases metabolism, Fabaceae enzymology, Plants, Medicinal
Abstract

DNA methylase activity was detected in nuclei from pea shoots. The enzyme can only be extracted by low-salt treatment if the nuclei are pretreated with micrococcal nuclease. Only a single enzyme was detected, and it was purified to a specific activity of 1620 units/mg of protein. It has an Mr of 160,000 on gel filtration and SDS/PAGE. Pea DNA methylase methylates cytosine in all four dinucleotides, and this is interpreted to show that it acts on CNG trinucleotides. Although it shows a strong preference for hemi-methylated double-stranded DNA, it is also capable of methylation de novo. Homologous DNA is the best natural substrate. In vitro the enzyme interacts with DNA to form a salt-resistant complex with DNA that is stable for at least 4 h.

Published
1991
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3. Eukaryotic DNA methylases and their use for in vitro methylation.

Author

Adams RL, Bryans M, Rinaldi A, Smart A, and Yesufu HM

Subjects
Animals, Base Sequence, Chromatography, Affinity, Chromatography, Gel, DNA Modification Methylases isolation & purification, Kinetics, Liver enzymology, Methylation, Mice, Molecular Sequence Data, Molecular Weight, Substrate Specificity, DNA Modification Methylases metabolism, Fabaceae enzymology, Plants, Medicinal
Abstract

DNA methylases from mouse and pea have been purified and characterized. Both are high molecular mass enzymes that show greater activity with hemimethylated than unmethylated substrate DNA. Both methylate cytosines in CpG preferentially, but not exclusively and show similar kinetics of methylation, which makes it difficult to saturate all possible sites on the DNA, but procedures are described that circumvent this problem.

Published
1990
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