318 results on '"Yi-Chen Li"'
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2. Repeated administration of adipose-derived mesenchymal stem cells added on beneficial effects of empagliflozin on protecting renal function in diabetic kidney disease rat
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Chih‐Chao Yang, Yi-Ling Chen, Pei-Hsun Sung, John Y. Chiang, Chih-Hung Chen, Yi-Chen Li, and Hon-Kan Yip
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Diabetic kidney disease ,Renal function ,Inflammation ,Oxidative stress ,Medicine (General) ,R5-920 ,Biology (General) ,QH301-705.5 - Abstract
Background: Diabetic kidney disease (DKD) is one of the most significant public health burdens worldwide. This study explored the renal protections of combined adipose-derived mesenchymal stem cells (ADMSCs) and empagliflozin (EMPA) in DKD rats. Methods: Adult-male-SD rats were equally allocated into group 1 (sham-operated-control), group 2 (DKD), group 3 (DKD + EMPA/20 mg/kg/day since day-14 after CKD-induction), group 4 [DKD + ADMSCs (6.0 × 105/intrarenal-arterial-injection/post-day-28, followed by 1.2 × 106/intravenous injection post-days 35 and 42 after CKD-induction, i.e., defined as repeated administration)] and group 5 (DKD + ADMSCs + EMPA) and kidney was harvested post-day-60 CKD-induction. Results: The result showed that the blood sugar and circulatory levels of BUN/creatinine and the ratio of urine protein/creatinine at day 60 were greatly increased in group 2 as compared the SC (i.e., group 1), significantly increased in groups 3 and 4 than in groups 5, but these parameters showed the similar manner in groups 3 and 4, except for blood sugar that was significantly lower in group 3 than in group 4 (all p
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- 2024
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3. Early and Dose-Dependent Xenogeneic Mesenchymal Stem Cell Therapy Improved Outcomes in Acute Respiratory Distress Syndrome Rodent Through Ameliorating Inflammation, Oxidative Stress, and Immune Reaction
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Kun-Chen Lin, Wen-Feng Fang, Pei-Hsun Sung, Kuo-Tung Huang, John Y. Chiang, Yi-Ling Chen, Chi-Ruei Huang, Yi-Chen Li, Mel S. Lee, and Hon-Kan Yip
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Medicine - Abstract
This study tested whether human umbilical cord–derived mesenchymal stem cells (HUCDMSCs) treatment effectively protected the rat lung against acute respiratory distress syndrome (ARDS) injury, and benefits of early and dose-dependent treatment. Rat pulmonary epithelial cell line L2 (PECL2) were categorized into G1 (PECL2), G2 (PECL2 + healthy rat lung-derived extraction/50 mg/ml co-cultured for 24 h), G3 (PECL2 + ARDS rat lung-derived extraction/50 mg/ml co-cultured for 24 h), and G4 (condition as G3 + HUCDMSCs/1 × 10 5 /co-cultured for 24 h). The result showed that the protein expressions of inflammatory (HMGB-1/TLR-2/TLR-4/MAL/TRAM/MyD88/TRIF/TRAF6/IkB/NF-κB/IL-1β/TNF-α), oxidative-stress/mitochondrial-damaged (NOX-1/NOX-2/ASK1/p-MKK4/p-MKK7/JNKs/JUN/cytosolic-cytochrome-C/cyclophilin-D/DRP1), and cell-apoptotic/fibrotic (cleaved-caspase 3/cleaved-PARP/TGF-β/p-Smad3) biomarkers were significantly increased in G3 than in G1/G2 and were significantly reversed in G4 (all P < 0.001), but they were similar between G1/G2. Adult male rats ( n = 42) were equally categorized into group 1 (normal control), group 2 (ARDS only), group 3 [ARDS + HUCDMSCs/1.2 × 10 6 cells intravenous administration at 3 h after 48 h ARDS induction (i.e., early treatment)], group 4 [ARDS + HUCDMSCs/1.2 × 10 6 cells intravenous administration at 24 h after 48 h ARDS induction (late treatment)], and group 5 [ARDS + HUCDMSCs/1.2 × 10 6 cells intravenous administration at 3 h/24 h after-48 h ARDS induction (dose-dependent treatment)]. By day 5 after ARDS induction, the SaO 2 %/immune regulatory T cells were highest in group 1, lowest in group 2, significantly lower in group 4 than in groups 3/5, and significantly lower in group 3 than in group 5, whereas the circulatory/bronchioalveolar lavage fluid inflammatory cells (CD11b-c+/LyG6+/MPO+)/circulatory immune cells (CD3-C4+/CD3-CD8+)/lung-leakage-albumin level/lung injury score/lung protein expressions of inflammatory (HMGB-1/TLR-2/TLR-4/MAL/TRAM/MyD88/TRIF/TRAF6/IκB-β/p-NF-κB/IL-1β/TNF-α)/fibrotic (p-SMad3/TGF-β), apoptosis (mitochondrial-Bax/cleaved-caspase-3)/oxidative-cell-stress (NOX-1/NOX-2/ASK1/p-MKK4/p-MKK7/p-JNKs/p-cJUN)/mitochondrial damaged (cyclophilin-D/DRP1/cytosolic-cytochrome-C) biomarkers displayed an opposite pattern of SaO 2 % among the groups (all P < 0.0001). Early administration was superior to and two-dose counterpart was even more superior to late HUCDMSCs treatment for protecting the lung against ARDS injury.
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- 2023
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4. Exposure to low levels of photocatalytic TiO2 nanoparticles enhances seed germination and seedling growth of amaranth and cruciferous vegetables
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Chi-Cheng Li, Sian-Ming Jhou, Yi-Chen Li, Jhih-Wei Ciou, You-Yen Lin, Shih-Che Hung, Jen-Hsiang Chang, Jen-Che Chang, Der-Shan Sun, Ming-Lun Chou, and Hsin-Hou Chang
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Medicine ,Science - Abstract
Abstract Titanium dioxide (TiO2) is one of the most common compounds on Earth, and it is used in natural forms or engineered bulks or nanoparticles (NPs) with increasing rates. However, the effect of TiO2 NPs on plants remains controversial. Previous studies demonstrated that TiO2 NPs are toxic to plants, because the photocatalytic property of TiO2 produces biohazardous reactive oxygen species. In contrast, another line of evidence suggested that TiO2 NPs are beneficial to plant growth. To verify this argument, in this study, we used seed germination of amaranth and cruciferous vegetables as a model system. Intriguingly, our data suggested that the controversy was due to the dosage effect. The photocatalytic activity of TiO2 NPs positively affected seed germination and growth through gibberellins in a plant-tolerable range (0.1 and 0.2 mg/cm2), whereas overdosing (1 mg/cm2) induced tissue damage. Given that plants are the foundations of the ecosystem; these findings are useful for agricultural application, sustainable development and maintenance of healthy environments.
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- 2022
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5. Safety and efficacy of intracoronary artery administration of human bone marrow-derived mesenchymal stem cells in STEMI of Lee-Sung pigs—A preclinical study for supporting the feasibility of the OmniMSC-AMI phase I clinical trial
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Wannhsin Chen, Chun-Hsiang Hou, Yi-Ling Chen, Hsin-Hsin Shen, Chen-Hsuan Lin, Cheng-Yi Wu, Meng-Hsueh Lin, Chih-Ching Liao, Jun-Jae Huang, Chi-Yu Yang, Yi-Chen Li, and Hon-Kan Yip
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acute myocardial infarction ,balloon occlusion ,exogenic mesenchymal stem cells ,porcine ,left ventricular ejection fraction ,fibrosis ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
BackgroundThis study tested whether early left intracoronary arterial (LAD) administration of human bone marrow-derived mesenchymal stem cells (hBMMSCs, called OmniMSCs) in acute ST-segment elevation myocardial infarction (STEMI) of Lee-Sung pigs induced by 90 min balloon-occluded LAD was safe and effective.Methods and resultsYoung male Lee-Sung pigs were categorized into SC (sham-operated control, n = 3), AMI-B (STEMI + buffer/21 cc/administered at 90 min after STEMI, n = 6), and AMI-M [acute myocardial infarction (AMI) + hBMMSCs/1.5 × 107/administered at 90 min after STEMI, n = 6] groups. By 2 and 5 months after STEMI, the cardiac magnetic resonance imaging demonstrated that the muscle scar score (MSS) and abnormal cardiac muscle exercise score in the infarct region were significantly increased in the AMI-B than in the SC group that were significantly reversed in the AMI-M group, whereas the left ventricular ejection function by each month (from 1 to 5) displayed an opposite pattern of MSS among the groups (all p 80%) was significantly earlier (estimated after 6-h AMI) in the AMI-M group than in the AMI-B group (p
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- 2023
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6. Cerebrospinal fluid metabolic profiling reveals divergent modulation of pentose phosphate pathway by midazolam, propofol and dexmedetomidine in patients with subarachnoid hemorrhage: a cohort study
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Yi-Chen Li, Rong Wang, Ji-Ye A, Run-Bin Sun, Shi-Jie Na, Tao Liu, Xuan-Sheng Ding, and Wei-Hong Ge
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Subarachnoid hemorrhage ,Sedation ,Midazolam ,Propofol ,Dexmedetomidine ,Metabolomics ,Anesthesiology ,RD78.3-87.3 - Abstract
Abstract Background Agitation is common in subarachnoid hemorrhage (SAH), and sedation with midazolam, propofol and dexmedetomidine is essential in agitation management. Previous research shows the tendency of dexmedetomidine and propofol in improving long-term outcome of SAH patients, whereas midazolam might be detrimental. Brain metabolism derangement after SAH might be interfered by sedatives. However, how sedatives work and whether the drugs interfere with patient outcome by altering cerebral metabolism is unclear, and the comprehensive view of how sedatives regulate brain metabolism remains to be elucidated. Methods For cerebrospinal fluid (CSF) and extracellular space of the brain exchange instantly, we performed a cohort study, applying CSF of SAH patients utilizing different sedatives or no sedation to metabolomics. Baseline CSF metabolome was corrected by selecting patients of the same SAH and agitation severity. CSF components were analyzed to identify the most affected metabolic pathways and sensitive biomarkers of each sedative. Markers might represent the outcome of the patients were also investigated. Results Pentose phosphate pathway was the most significantly interfered (upregulated) pathway in midazolam (p = 0.0000107, impact = 0.35348) and propofol (p = 0.00000000000746, impact = 0.41604) groups. On the contrary, dexmedetomidine decreased levels of sedoheptulose 7-phosphate (p = 0.002) and NADP (p = 0.024), and NADP is the key metabolite and regulator in pentose phosphate pathway. Midazolam additionally augmented purine synthesis (p = 0.00175, impact = 0.13481) and propofol enhanced pyrimidine synthesis (p = 0.000203, impact = 0.20046), whereas dexmedetomidine weakened pyrimidine synthesis (p = 0.000000000594, impact = 0.24922). Reduced guanosine diphosphate (AUC of ROC 0.857, 95%CI 0.617–1, p = 0.00506) was the significant CSF biomarker for midazolam, and uridine diphosphate glucose (AUC of ROC 0.877, 95%CI 0.631–1, p = 0.00980) for propofol, and succinyl-CoA (AUC of ROC 0.923, 95%CI 0.785–1, p = 0.000810) plus adenosine triphosphate (AUC of ROC 0.908, 95%CI 0.6921, p = 0.00315) for dexmedetomidine. Down-regulated CSF succinyl-CoA was also associated with favorable outcome (AUC of ROC 0.708, 95% CI: 0.524–0.865, p = 0.029333). Conclusion Pentose phosphate pathway was a crucial target for sedatives which alter brain metabolism. Midazolam and propofol enhanced the pentose phosphate pathway and nucleotide synthesis in poor-grade SAH patients, as presented in the CSF. The situation of dexmedetomidine was the opposite. The divergent modulation of cerebral metabolism might further explain sedative pharmacology and how sedatives affect the outcome of SAH patients.
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- 2022
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7. Synergic Effect of Combined Therapy of Hyperbaric Oxygen and Adipose-Derived Mesenchymal Stem Cells on Improving Locomotor Recovery After Acute Traumatic Spinal Cord Injury in Rat Mainly Through Downregulating Inflammatory and Cell-Stress Signalings
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Tsung-Cheng Yin, Pei-Lin Shao, Kuan-Hung Chen, Kun-Chen Lin, John Y. Chiang, Pei-Hsun Sung, Shun-Cheng Wu, Yi-Chen Li, Hon-Kan Yip, and Mel S. Lee
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Medicine - Abstract
This study tested whether combined hyperbaric oxygen (HBO) and allogenic adipose-derived mesenchymal stem cells (ADMSCs) would be superior to either one for improving the locomotor recovery in rat after acute traumatic spinal cord injury (TSCI) in rat. Adult-male Sprague–Dawley rats were equally categorized into group 1 (sham-operated control), group 2 (TSCI), group 3 (TSCI + HBO for 1.5 h/day for 14 consecutive days after TSCI), group 4 (TSCI + ADMSCs/1.2 × 10 6 cells by intravenous injection at 3 h and days 1/2 after TSCI), and group 5 (TSCI + HBO + ADMSCs), euthanized, and spinal cord tissue was harvested by day 49 after TSCI. The protein expressions of oxidative-stress (NOX-1/NOX-2), inflammatory-signaling (TLR-4/MyD88/IL-1β/TNF-α/substance-p), cell-stress signaling (PI3K/p-AKT/p-mTOR), and the voltage-gated sodium channel (Nav1.3/1.8/1.9) biomarkers were highest in group 2, lowest in group 1, and significantly lower in group 5 than in groups 3/4 (all P
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- 2022
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8. Dissecting the Roles of PDCD4 in Breast Cancer
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Qian Cai, Hsin-Sheng Yang, Yi-Chen Li, and Jiang Zhu
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PDCD4 ,breast cancer ,translational control ,drug resistance ,tumor suppressor ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
The human programmed cell death 4 (PDCD4) gene was mapped at chromosome 10q24 and encodes the PDCD4 protein comprised of 469 amino acids. PDCD4 inhibits protein translation PDCD4 inhibits protein translation to suppress tumor progression, and its expression is frequently decreased in breast cancer. PDCD4 blocks translation initiation complex by binding eIF4A via MA-3 domains or by directly binding 5’ mRNA internal ribosome entry sites with an RNA binding domain to suppress breast cancer progression and proliferation. Numerous regulators and biological processes including non-coding RNAs, proteasomes, estrogen, natural compounds and inflammation control PDCD4 expression in breast cancer. Loss of PDCD4 expression is also responsible for drug resistance in breast cancer. HER2 activation downregulates PDCD4 expression by activating MAPK, AKT, and miR-21 in aromatase inhibitor-resistant breast cancer cells. Moreover, modulating the microRNA/PDCD4 axis maybe an effective strategy for overcoming chemoresistance in breast cancer. Down-regulation of PDCD4 is significantly associated with short overall survival of patients, which suggests that PDCD4 may be an independent prognostic marker for breast cancer.
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- 2022
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9. Dipeptidyl peptidase 4 promotes peritoneal fibrosis and its inhibitions prevent failure of peritoneal dialysis
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Yi-Chen Li, Pei-Hsun Sung, Yao-Hsu Yang, John Y. Chiang, Hon-Kan Yip, and Chih‐Chao Yang
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Biology (General) ,QH301-705.5 - Abstract
Li et al. show that dipeptidyl peptidase 4 (DPP4) promotes peritoneal fibrosis whereas the inhibition of DPP4 protects the patients from failure of peritoneal dialysis. This study provides mechanistic insights into the effects of DPP4 on peritoneal fibrosis and the translational potential of these effects.
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- 2021
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10. Safety and efficacy of intrarenal arterial autologous CD34+ cell transfusion in patients with chronic kidney disease: A randomized, open‐label, controlled phase II clinical trial
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Chih‐Chao Yang, Pei‐Hsun Sung, Ben‐Chung Cheng, Yi‐Chen Li, Yi‐Ling Chen, Mel S. Lee, and Hon‐Kan Yip
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angiogenesis ,CD34+ cell therapy ,chronic kidney disease ,circulating endothelial progenitor cells ,Medicine (General) ,R5-920 ,Cytology ,QH573-671 - Abstract
Abstract Background This was a randomized, open‐label, controlled phase II clinical trial to investigate the safety, efficacy, and outcomes of intrarenal artery infusion of autologous peripheral‐blood‐derived CD34+ cells for patients with chronic kidney disease (CKD; ie, stage III or IV). Materials and Methods Between October 2016 and July 2018, 52 consecutive patients with CKD at stage III or IV were randomly allocated into a treatment group (TG; 2.5 × 107 cells for each intrarenal artery; n = 26) and a control group (CG; standardized pharmacotherapy only; n = 26). The primary endpoints included safety and change of creatinine level/creatinine clearance. The secondary endpoints were 12‐month combined unfavorable clinical outcomes (defined as dialysis or death), improvement in proteinuria, and CD34+ cell‐related adverse events. Results All patients were uneventfully discharged after CD34+ cell therapy. The baseline endothelial progenitor cell (EPC) populations did not differ between TG and CG (P > .5). Flow cytometric analysis showed increases in circulating EPC (ie, CD34+KDR+CD45dim/ CD34+CD133+CD45dim/CD31+CD133+CD45dim/CD34+CD133+KDR+/CD133+) and hematopoietic stem cell (CD34+) populations after granulocyte‐colony stimulating factor treatment (all P .1). Conclusion CD34+ cell therapy was safe and improved 1‐year outcome.
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- 2020
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11. Baseline factors identified for the prediction of good responders in patients with end-stage diffuse coronary artery disease undergoing intracoronary CD34+ cell therapy
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Pei-Hsun Sung, Hsin-Ju Chiang, Yi-Chen Li, John Y. Chiang, Chi-Hsiang Chu, Pei-Lin Shao, Fan-Yen Lee, Mel S. Lee, and Hon-Kan Yip
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CD34+ cell therapy ,Good responders ,Diffuse coronary artery disease ,Refractory angina ,Left ventricular ejection fraction ,Medicine (General) ,R5-920 ,Biochemistry ,QD415-436 - Abstract
Abstract Background Treating patients with end-stage diffuse coronary artery disease (EnD-CAD) unsuitable for coronary intervention remains a clinical challenge. They usually express refractory angina and have a high risk of mortality. Although growing data have indicated cell therapy is an alternative solution to medical or invasive therapy, there are still lacking useful markers to predict whether heart function will improve in the EnD-CAD patients who underwent circulatory-derived CD34+ cell therapy. By utilizing the baseline variables and results from our previous phase I/II clinical trials, the aim of this study tried to elucidate the variables predictive of the “good response” to CD34+ cell therapy. Methods This retrospective study included 38 patients in phase I clinical trial (2011–2014), and 30 patients in phase II clinical trial (2013–2017). These patients were categorized into “good responders” and “non-responders” according to their 1-year improvement of LVEF ≥ 7.0% or
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- 2020
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12. Intra-carotid arterial transfusion of circulatory-derived autologous endothelial progenitor cells in rodent after ischemic stroke—evaluating the impact of therapeutic time points on prognostic outcomes
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Kun-Chen Lin, Han-Tan Chai, Kuan-Hung Chen, Pei-Hsun Sung, John Y. Chiang, Pei-Lin Shao, Chi-Ruei Huang, Yi-Chen Li, Sheung-Fat Ko, and Hon-Kan Yip
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Ischemic stroke ,Endothelial progenitor cells ,Angiogenesis ,Neurological function ,Medicine (General) ,R5-920 ,Biochemistry ,QD415-436 - Abstract
Abstract Background This study tested the optimal time point for left intra-carotid arterial (LICA) administration of circulatory-derived autologous endothelial progenitor cells (EPCs) for improving the outcome in rat after acute ischemic stroke (IS). Methods and results Adult male SD rats (n = 70) were equally categorized into group 1 (sham-operated control), group 2 (IS), group 3 (IS+EPCs/1.2 × 106 cells/by LICA administration 3 h after IS), group 4 (IS+EPCs/LICA administration post-day-3 IS), group 5 (IS+EPCs/LICA administration post-day-7 IS), group 6 (IS+EPCs/LICA administration post-day-14 IS), and group 7 (IS+EPCs/LICA administration post-day-28 IS). The brain infarct volume (BIV) (at day 60/MRI) was lowest in group 1, highest in group 2, and significantly progressively increased from groups 3 to 7, whereas among the IS animals, the neurological function was significantly preserved in groups 3 to 6 than in groups 2 and 7 post-day-60 IS (all P
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- 2020
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13. An AlGaN/GaN High Electron Mobility Transistor With a Built-In Light Emitter Using Radiative Recombination of Two-Dimensional Electron Gas and Holes
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Chih-Yao Chang, Yi-Chen Li, Kailin Ren, Yung C. Liang, and Chih-Fang Huang
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GaN ,HEMT ,light emitting devices ,two-dimensional electron gas ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 - Abstract
This paper reports a novel HEMT structure that includes a built-in light emitter through band-to-band radiative recombination that is provided via holes from the p-GaN layer and electrons from the 2DEG. The electrical switching and illumination functions are able to be combined into a single device. The peak of the emitted light spectrum is located at 365 nm, corresponding to the bandgap of the GaN layer. This device uses a simple and cost-effective process, and shows the possibility for use in applications such as optical interconnects and optoelectronic integrated circuits.
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- 2020
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14. Biomechanical Effect of Hybrid Dynamic Stabilization Implant on the Segmental Motion and Intradiscal Pressure in Human Lumbar Spine
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Chih-Kun Hsiao, Yi-Jung Tsai, Cheng-Yo Yen, Yi-Chen Li, Hao-Yuan Hsiao, and Yuan-Kun Tu
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dynamic stabilization systems ,Dynesys-Transition-Optima ,range of motion ,intradiscal pressure ,Technology ,Biology (General) ,QH301-705.5 - Abstract
The hybrid dynamic stabilization system, Dynesys-Transition-Optima, represents a novel pedicle-based construct for the treatment of lumbar degenerative disease. The theoretical advantage of this system is to stabilize the treated segment and preserve the range of motion within the adjacent segment while potentially decreasing the risk of adjacent segment disease following lumbar arthrodesis. Satisfactory short-term outcomes were previously demonstrated in the Dynesys-Transition-Optima system. However, long-term follow-up reported accelerated degeneration of adjacent segments and segmental instability above the fusion level. This study investigated the biomechanical effects of the Dynesys-Transition-Optima system on segment motion and intradiscal pressure at adjacent and implanted levels. Segmental range of motion and intradiscal pressure were evaluated under the conditions of the intact spine, with a static fixator at L4–5, and implanted with DTO at L3–4 (Dynesys fixator) and L4–5 (static fixator) by applying the loading conditions of flexion/extension (±7.5 Nm) and lateral bending (±7.5 Nm), with/without a follower preload of 500 N. Our results showed that the hybrid Dynesys-Transition-Optima system can significantly reduce the ROM at the fusion level (L4–L5), whereas the range of motion at the adjacent level (L3–4) significantly increased. The increase in physiological loading could be an important factor in the increment of IDP at the intervertebral discs at the lumbar spine. The Dynesys-Transition-Optima system can preserve the mobility of the stabilized segments with a lesser range of motion on the transition segment; it may help to prevent the occurrence of adjacent segment degeneration. However, the current study cannot cover all the issues of adjacent segmental diseases. Future investigations of large-scale and long-term follow-ups are needed.
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- 2022
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15. Infection and image findings to predict delayed hemorrhage in postoperative pancreatic fistula patients after pancreaticoduodenectomy
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Jie Yang, Yi-Chen Li, Xu-Bao Liu, and Chun-Lu Tan
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Pancreaticoduodenectomy ,Pancreatic fistula ,Postoperative hemorrhage ,Surgery ,RD1-811 - Published
- 2022
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16. Synergic Effect of Early Administration of Probiotics and Adipose-Derived Mesenchymal Stem Cells on Alleviating Inflammation-Induced Chronic Neuropathic Pain in Rodents
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Kuan-Hung Chen, Hung-Sheng Lin, Yi-Chen Li, Pei-Hsun Sung, Yi-Ling Chen, Tsung-Cheng Yin, and Hon-Kan Yip
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neuropathic pain ,chronic constriction injury ,probiotics ,adipose-tissue-derived mesenchymal stem cells ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
This study investigated the hypothesis that probiotics enhanced the therapeutic effect of adipose-derived mesenchymal stem cells (ADMSCs) on alleviating neuropathic pain (NP) due to chronic constriction injury (CCI) mainly through regulating the microbiota in rats. SD rats (n = 50) were categorized into group 1 (sham-control), group 2 (NP), group 3 (NP + probiotics (i.e., 1.5 billion C.F.U./day/rat, orally 3 h after NP procedure, followed by QOD 30 times)), group 4 (NP + ADMSCs (3.0 × 105 cells) 3 h after CCI procedure, followed by QOD six times (i.e., seven times in total, i.e., mimic a clinical setting of drug use) and group 5 (NP + probiotics + ADMSCs (3.0 × 105 cells)) and euthanized by day 60 after NP induction. By day 28 after NP induction, flow-cytometric analysis showed circulating levels of early (AN-V+/PI−) and late (AN-V+/PI+) apoptotic, and three inflammatory (CD11b-c+, Ly6G+ and MPO+) cells were lowest in group 1 and significantly progressively reduced in groups 2 to 5 (all p < 0.0001). By days 7, 14, 21, 28, and 60 after CCI, the thresholds of thermal paw withdrawal latency (PWL) and mechanical paw withdrawal threshold (PWT) were highest in group 1 and significantly progressively increased in groups 2 to 5 (all p < 0.0001). Numbers of pain-connived cells (Nav1.8+/peripherin+, p-ERK+/peripherin+, p-p38+/peripherin+ and p-p38+/NF200+) and protein expressions of inflammatory (p-NF-κB, IL-1ß, TNF-α and MMP-9), apoptotic (cleaved-caspase-3, cleaved-PARP), oxidative-stress (NOX-1, NOX-2), DNA-damaged (γ-H2AX) and MAPK-family (p-P38, p-JNK, p-ERK1/2) biomarkers as well as the protein levels of Nav.1.3, Nav.1.8, and Nav.1.9 in L4-L5 in dorsal root ganglia displayed an opposite pattern of mechanical PWT among the groups (all p < 0.0001). In conclusion, combined probiotic and ADMSC therapy was superior to merely one for alleviating CCI-induced NP mainly through suppressing inflammation and oxidative stress.
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- 2022
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17. Clinical utility of mean platelet volume and immature platelet fraction in acute coronary syndromeAt a glance commentary
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Hsien-Li Huang, Chih-Hung Chen, Chia-Te Kung, Yi-Chen Li, Pei-Hsun Sung, Huey-Ling You, Yu-Hung Lin, and Wan-Ting Huang
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Medicine (General) ,R5-920 ,Biology (General) ,QH301-705.5 - Abstract
Background: Platelets play an important role in the pathogenesis of acute coronary syndrome (ACS). Patients with ACS have an increased mean platelet volume (MPV) and immature platelet fraction (IPF) resulting in elevation of thrombotic ability. In this study, we evaluated the diagnostic performance of MPV and IPF in identifying suspected ACS patients at emergency department. Moreover, we investigated the correlation between MPV or IPF with initial troponin I (TnI), one of the current ACS biomarkers. Methods: This was a single-center study recruiting suspected ACS patients who had acute chest pain at the emergency department. Whole blood samples were obtained from all participants and MPV and IPF were measured by Sysmex XE-5000 hematology analyzer within 20 min of blood sampling. The diagnostic values of MPV and IPF in identifying ACS were analyzed retrospectively. Result: In this study, 63 in 104 suspected ACS patients were diagnosed as ACS (65.3%). MPV and IPF were higher in ACS patients compared to non-ACS patients (MPV: 10.7 ± 0.80 fL vs 10.0 ± 0.64 fL, p
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- 2019
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18. Sitagliptin and shock wave-supported peripheral blood derived endothelial progenitor cell therapy effectively preserves residual renal function in chronic kidney disease in rat—role of dipeptidyl peptidase 4 inhibition
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Pei-Hsun Sung, Kuan-Hung Chen, Yi-Chen Li, John Y. Chiang, Mel S. Lee, and Hon-Kan Yip
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Chronic kidney disease ,High-protein diet ,Shock wave ,Sitagliptin ,Endothelial progenitor cells ,Therapeutics. Pharmacology ,RM1-950 - Abstract
This study tested whether sitagliptin and shock wave (SW)-assisted circulatory-derived autologous endothelial progenitor cell (EPC) therapy would effectively preserve residual renal function in chronic kidney disease (CKD) induced by 5/6 left-nephrectomy/remove right kidney plus daily feeding high-protein diet (HPD) in rat. Adult-male SD rats (n = 40) were categorized into group 1 (sham-operated control with HPD), group 2 (HPD-CKD), group 3 [HPD-CKD + EPC (1.2 × 106 cell)/intra-vessel administration by day 14 after CKD-induction], group 4 [HPD-CKD + SW (0.12 mJ/mm2/180 shorts) at days 14/21/28 after CKD-induction by ultrasound-guided application] and group 5 [HPD-CKD + SW + EPC + sitagliptin (Sita; 600 mg/kg/day since day 14 after CKD induction)]. All animals were euthanized by day 60. By day 60, renal blood flow (RBF) was highest in group 1 and progressively increased from groups 2 to 5, whereas the levels of creatinine/BUN/proteinuria exhibited an opposite pattern of RBF among the five groups (all p
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- 2019
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19. Early administration of empagliflozin preserved heart function in cardiorenal syndrome in rat
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Chih-Chao Yang, Yen-Ta Chen, Christopher Glenn Wallace, Kuan-Hung Chen, Ben-Chung Cheng, Pei-Hsun Sung, Yi-Chen Li, Sheung-Fat Ko, Hsueh-Wen Chang, and Hon-Kan Yip
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Empagliflozin ,Cardiorenal syndrome ,Heart function ,Inflammation ,Oxidative stress ,Therapeutics. Pharmacology ,RM1-950 - Abstract
This study tested the hypothesis that early administration of empagliflozin (Empa), an inhibitor of glucose recycling in renal tubules, could preserve heart function in cardiorenal syndrome (CRS) in rat. Chronic kidney disease (CKD) was caused by 5/6 subtotal nephrectomy and dilated cardiomyopathy (DCM) by doxorubicin (DOX) treatment. In vitro results showed that protein expressions of cleaved-caspase3 and autophagy activity at 24 h/48 h in NRK-52P cells were significantly upregulated by para-Creso treatment; these were significantly downregulated by Empa treatment. Flow cytometric analysis showed that annexin-V (i.e., early/late apoptosis) in NRK-52P cells expressed an identical pattern to cleaved-caspase3 between the two groups (all p
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- 2019
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20. Anticoagulation Resumption in a Patient With Mechanical Heart Valves, Antithrombin Deficiency, and Hemorrhagic Transformation Following Thrombectomy After Ischemic Stroke
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Yi-Chen Li, Rong Wang, Hang Xu, Lan-Ping Ding, and Wei-Hong Ge
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anticoagulation ,argatroban ,hemorrhagic transformation (HT) ,valve thrombosis ,thrombectomy ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Anticoagulation is essential for patients undergoing mechanical heart valve replacement; however, the timing to reinitiate the anticoagulant could be a dilemma that imposes increased risk for bleeding events in patients suffering from the life-threatening hemorrhagic transformation (HT) after ischemic stroke. Such a situation was presented in this case report. A 71-year-old woman was transferred directly to the Neurocritical Care Unit because of a HT that occurred following the mechanical thrombectomy for ischemic stroke. Since she had a history of prosthetic metallic valve replacement, how the anticoagulating therapy could balance the hemorrhagic and thrombotic risks was carefully evaluated. On day 6 after the onset of hemorrhage transformation, the laboratory results of coagulation and fibrinolysis strongly suggested thrombosis as well as antithrombin deficiency. The short-acting and titratable anticoagulant argatroban was immediately initiated at low dose, and thrombosis was temporarily terminated. On day 3 of anticoagulation resumption, argatroban was discontinued for one dose when the prothrombin time and activated partial thromboplastin time significantly prolonged after argatroban infusion. Aortic valve thrombosis was detected the next day. The anticoagulation was then strengthened by dose adjustment to keep mitral valve intact, to stabilize the aortic valve thrombosis, and to decrease the aortic flow rate. The intravenous argatroban was transited to oral warfarin before the patient was discharged. This study is the first report of administering argatroban and titrating to its appropriate dose in the patient with valve thrombosis, antithrombin deficiency, and HT after mechanical thrombectomy for acute ischemic stroke. Notably, the fluctuations argatroban brings to the coagulation test results might not be interpreted as increased bleeding risk. This case also suggested that the reported timing (day 6 to day 14 after hemorrhage) of anticoagulant resumption in primary intracerebral hemorrhage with mechanical valves might be late for some patients with HT.
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- 2020
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21. Knockout of KLF10 Ameliorated Diabetic Renal Fibrosis via Downregulation of DKK-1
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Yung-Chien Hsu, Cheng Ho, Ya-Hsueh Shih, Wen-Chiu Ni, Yi-Chen Li, Hsiu-Ching Chang, and Chun-Liang Lin
- Subjects
diabetes ,renal fibrosis ,KLF10 ,DKK-1 ,Organic chemistry ,QD241-441 - Abstract
Diabetes-induced chronic kidney disease leads to mortality and morbidity and thus poses a great health burden worldwide. Krüppel-like factor 10 (KLF10), a zinc finger-containing transcription factor, regulates numerous cellular functions, such as proliferation, differentiation, and apoptosis. In this study, we explored the effects of KLF10 on diabetes-induced renal disease by using a KLF10 knockout mice model. Knockout of KLF10 obviously diminished diabetes-induced tumor growth factor-β (TGF-β), fibronectin, and type IV collagen expression, as evidenced by immunohistochemical staining. KLF10 knockout also repressed the expression of Dickkopf-1 (DKK-1) and phosphorylated β-catenin in diabetic mice, as evidenced by immunohistochemical staining and Western blot analysis. Quantitative reverse transcriptase–polymerase chain reaction (RT-PCR) revealed that significantly decreased type IV collagen, fibronectin, and DKK-1 existed in KLF10 knockout diabetic mice compared with control diabetic mice. Moreover, knockout of KLF10 reduced the renal fibrosis, as shown by Masson’s Trichrome analysis. Overall, the results indicate that depletion of KLF10 ameliorated diabetic renal fibrosis via the downregulation of DKK-1 expression and inhibited TGF-β1 and phosphorylated β-catenin expression. Our findings suggest that KLF10 may be a promising therapeutic choice for the treatment of diabetes-induced renal fibrosis.
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- 2022
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22. Human Umbilical Cord–Derived Mesenchymal Stem Cell Therapy Effectively Protected the Brain Architecture and Neurological Function in Rat After Acute Traumatic Brain Injury
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Kuan-Hung Chen, Pei-Lin Shao, Yi-Chen Li, John Y. Chiang, Pei-Hsun Sung, Hui-Wen Chien, Fu-Yuan Shih, Mel S. Lee, Wu-Fu Chen, and Hon-Kan Yip
- Subjects
Medicine - Abstract
Intracranial hemorrhage from stroke and head trauma elicits a cascade of inflammatory and immune reactions detrimental to neurological integrity and function at cellular and molecular levels. This study tested the hypothesis that human umbilical cord–derived mesenchymal stem cell (HUCDMSC) therapy effectively protected the brain integrity and neurological function in rat after acute traumatic brain injury (TBI). Adult male Sprague-Dawley rats ( n = 30) were equally divided into group 1 (sham-operated control), group 2 (TBI), and group 3 [TBI + HUCDMSC (1.2 × 10 6 cells/intravenous injection at 3 h after TBI)] and euthanized by day 28 after TBI procedure. The results of corner test and inclined plane test showed the neurological function was significantly progressively improved from days 3, 7, 14, and 28 in groups 1 and 3 than in group 2, and group 1 than in group 3 (all P < 0.001). By day 28, brain magnetic resonance imaging brain ischemic volume was significantly increased in group 2 than in group 3 ( P < 0.001). The protein expressions of apoptosis [mitochondrial-bax positive cells (Bax)/cleaved-caspase3/cleaved-poly(adenosine diphosphate (ADP)-ribose) polymerase], fibrosis (Smad3 positive cells (Smad3)/transforming growth factor-β), oxidative stress (NADPH Oxidase 1 (NOX-1)/NADPH Oxidase 2 (NOX-2)/oxidized-protein/cytochrome b-245 alpha chain (p22phox)), and brain-edema/deoxyribonucleic acid (DNA)–damaged biomarkers (Aquaporin-4/gamma H2A histone family member X ( (γ-H2AX)) displayed an identical pattern to neurological function among the three groups (all P < 0.0001), whereas the protein expressions of angiogenesis biomarkers (vascular endothelial growth factor/stromal cell–derived factor-1α/C-X-C chemokine receptor type 4 (CXCR4)) significantly increased from groups 1 to 3 (all P < 0.0001). The cellular expressions of inflammatory biomarkers (cluster of differentiation 14 (+) cells (CD14+)/glial fibrillary acidic protein positive cells (GFAP+)/ a member of a new family of EGF-TM7 molecules positive cells (F4/80+)) and DNA-damaged parameter (γ-H2AX) exhibited an identical pattern, whereas cellular expressions of neural integrity (hexaribonucleotide Binding Protein-3 positive cells (NeuN+)/nestin+/doublecortin+) exhibited an opposite pattern of neurological function among the three groups (all P < 0.0001). Xenogeneic HUCDMSC therapy was safe and it significantly preserved neurological function and brain architecture in rat after TBI.
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- 2020
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23. Hyaluronic acid on the urokinase sustained release with a hydrogel system composed of poloxamer 407: HA/P407 hydrogel system for drug delivery.
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Hao-Ying Hsieh, Wei-Yang Lin, An Li Lee, Yi-Chen Li, Yi-Jane Chen, Ke-Cheng Chen, and Tai-Horng Young
- Subjects
Medicine ,Science - Abstract
Pleural empyema is an inflammatory condition characterized by accumulation of pus inside the pleural cavity, which is usually followed by bacterial pneumonia. During the disease process, the pro-inflammatory and pro-fibrotic cytokines in the purulent pleural effusion cause proliferation of fibroblasts and deposition of extracellular matrix, which lead to fibrin deposition and fibrothorax. Urokinase instillation therapy through a chest drainage tube is frequently used for fibrinolysis in patients with empyema. However, urokinase treatment requires multiple instillation (2-3 times per day, for 4-8 days) and easily flows out from the chest drainage tube due to its high water solubility. In this in vitro study, we developed a thermo-responsive hydrogel based on poloxamer 407 (P407) combined with hyaluronic acid (HA) for optimal loading and release of urokinase. Our results show that the addition of HA to poloxamer gels provides a significantly more compact microstructure, with smaller pore sizes (**p < 0.001). The differential scanning calorimetry (DSC) profile revealed no influence on the micellization intensity of poloxamer gel by HA. The 25% poloxamer-based gel was significantly superior to the 23% poloxamer-based gel, with slower gel erosion when comparing the 16th hour residual gel weight of both gels (*p < 0.05; **p < 0.001). The 25% poloxamer-HA gel also exhibited a superior urokinase release profile and longer release time. A Fourier-transform infrared spectroscopy (FT-IR) study of the P407/HA hydrogel showed no chemical interactions between P407 and HA in the hydrogel system. The thermoresponsive P407/HA hydrogel may have a promising potential in the loading and delivery of hydrophilic drugs. On top of that, in vitro toxicity test of this combination demonstrates a lower toxicity.
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- 2020
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24. Experimental evaluation of stiffening effect induced by UVA/Riboflavin corneal cross-linking using intact porcine eye globes.
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Shao-Hsuan Chang, Dong Zhou, Ashkan Eliasy, Yi-Chen Li, and Ahmed Elsheikh
- Subjects
Medicine ,Science - Abstract
UVA/riboflavin corneal cross-linking (CXL) is a common used approach to treat progressive keratoconus. This study aims to investigate the alteration of corneal stiffness following CXL by mimicking the inflation of the eye under the in vivo loading conditions. Seven paired porcine eye globes were involved in the inflation test to examine the corneal behaviour. Cornea-only model was constructed using the finite element method, without considering the deformation contribution from sclera and limbus. Inverse analysis was conducted to calibrate the non-linear material behaviours in order to reproduce the inflation test. The corneal stress and strain values were then extracted from the finite element models and tangent modulus was calculated under stress level at 0.03 MPa. UVA/riboflavin cross-linked corneas displayed a significant increase in the material stiffness. At the IOP of 27.25 mmHg, the average displacements of corneal apex were 307 ± 65 μm and 437 ± 63 μm (p = 0.02) in CXL and PBS corneas, respectively. Comparisons performed on tangent modulus ratios at a stress of 0.03 MPa, the tangent modulus measured in the corneas treated with the CXL was 2.48 ± 0.69, with a 43±24% increase comparing to its PBS control. The data supported that corneal material properties can be well-described using this inflation methods following CXL. The inflation test is valuable for investigating the mechanical response of the intact human cornea within physiological IOP ranges, providing benchmarks against which the numerical developments can be translated to clinic.
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- 2020
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25. Meta analysis of traditional Chinese medicine in the treatment of dry eye in recent years
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Peng-Fei Jiang, Jun Peng, Ya-Sha Zhou, Xiao-Fang Peng, Xiang Li, Yi-Chen Li, Qing-Hua Peng, and Xiang-Dong Chen
- Subjects
dry eye ,traditional Chinese medicine ,Meta analysis ,randomized controlled trials ,Ophthalmology ,RE1-994 - Abstract
AIM: To evaluate the traditional Chinese medicine(TCM)for the treatment of dry eye effect in the past five years by using meta analysis method. METHODS: According to the Cochrane evaluation system method, we searched Medline(January 2013 to October 2017), EMbase(2013-2017), Cochrane Library(2017), Wanfang database(2013-2017), VIP(2013-2017), and CNKI(2013-2017)for studies published. We included randomized controlled trials conducted the TCM in the treatment of dry eye. RevMan 5.0 statistical software data extraction and Meta analysis were conducted. RESULTS: A total of 10 studies were identified, nine were from Chinese literature and one was from English literature, of which including 1 229 eyes. Nine of these studies performed BUT measurements at the end of the course of treatment. The results showed a statistically significant difference(PPP=0.25). Three studies performed dry eye symptoms after the end of the course of treatment; the results showed that the differences were statistically significant(P=0.0003); the overall efficacy comparison, the difference between the two groups was statistically significant(PCONCLUSION: TCM treatment can significantly prolong BUT and increase tear flow, and has more advantages in the treatment of dry eye.
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- 2018
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26. Ophthalmic Syndrome Differentiation System and Digital Chinese Medicine
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Peng-Fei Jiang, Jun Peng, Ya-Sha Zhou, Yi-Chen Li, Qing-Hua Peng, and Xiang-Dong Chen
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Medicine ,Other systems of medicine ,RZ201-999 - Abstract
ABSTRACT: In traditional Chinese medicine (TCM), ophthalmic syndrome differentiation is an ophthalmology-specific method for identifying syndromes based on the “Five Orbiculi” theory. It was devised by Professor Qing-Hua PENG through an unprecedented combination of syndrome element differentiation and ophthalmic clinical practices, based on the Clinical Terminology of Chinese Medical Diagnosis and Treatment—Syndromes of the National Standards of the People’s Republic of China. This approach integrates an ophthalmic syndrome differentiation system with digital Chinese medicine (DCM), and proposes the extraction of syndrome elements of ophthalmic diseases from research on DCM. These elements are then quantified and organized to form a model of digital diagnosis and treatment specific to ophthalmology, which should help to achieve synergistic development of the ophthalmic syndrome differentiation system and DCM. Keywords: Ophthalmic syndrome differentiation, Digital Chinese medicine (DCM), Syndrome element differentiation, Dialectic of traditional Chinese Medicine, Five Orbiculi
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- 2018
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27. Extracorporeal Shock Wave Enhanced Exogenous Mitochondria into Adipose-Derived Mesenchymal Stem Cells and Further Preserved Heart Function in Rat Dilated Cardiomyopathy
- Author
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Pei-Hsun Sung, Mel S. Lee, Han-Tan Chai, John Y. Chiang, Yi-Chen Li, Yi-Ching Chu, Chi-Ruei Huang, and Hon-Kan Yip
- Subjects
exogenous mitochondria delivery ,dilated cardiomyopathy ,extracorporeal shock wave ,left ventricular ejection fraction ,angiogenesis ,Biology (General) ,QH301-705.5 - Abstract
This study tested whether extracorporeal shock wave (ECSW) supported-exogenous mitochondria (Mito) into adipose-derived mesenchymal stem cells (ADMSCs) would preserve left-ventricular-ejection-fraction (LVEF) in doxorubicin/12 mg/kg-induced dilated cardiomyopathy (DCM) rat. Adult-male-SD rats were equally categorized into group 1 (sham-control), group 2 (DCM), group 3 (DCM + ECSW/1.5 mJ/mm2 for 140 shots/week × 3 times/since day 14 after DCM induction), group 4 (DCM + ECSW/1.5 mJ/mm2/100 shots-assisted mito delivery (500 μg) into ADMSCs/1.2 × 106 cells, then implanted into LV myocardium day 14 after DCM induction) and group 5 (DCM + ECSW-assisted mito delivery into ADMSCs/1.2 × 106 cells, then implanted into LV, followed by ECSW/1.5 mJ/mm2 for 140 shots/week × 3 times/since day 14 after DCM induction) and euthanized by day 49. Microscopic findings showed mitochondria were abundantly enhanced by ECSW into H9C2 cells. The q-PCR showed a significant increase in relative number of mitDNA in mitochondrial-transferred H9C2 cells than in control group (p < 0.01). The angiogenesis/angiogenesis factors (VEGF/SDF-1α/IG-F1) in HUVECs were significantly progressively increased by a stepwise-increased amount of ECSW energy (0.1/0.25/0.35 mJ/mm2) (all p < 0.001). The 49-day LVEF was highest in group 1 and significantly progressively increased from groups 2 to 5 (all p < 0.0001). Cardiomyocyte size/fibrosis exhibited an opposite pattern of LVEF, whereas cellular/protein levels of angiogenesis factors (VEGF/SDF-1α) in myocardium were significantly progressively increased from groups 1 to 5 (all p < 0.0001). The protein expressions of apoptotic/mitochondrial (cleaved-caspase-3/cleaved-PARP/mitochondrial-Bax/cytosolic-cytochrome-C), fibrotic (p-Smad3/TGF-ß), oxidative-stress (NOX-1/NOX-2) and pressure-overload/heart failure (BNP/ß-MHC) biomarkers exhibited an opposite pattern of LVEF among the five groups (all p < 0.0001). ECSW-assisted mitochondrial-delivery into ADMSCs plus ECSW offered an additional benefit for preserving LVEF in DCM rat.
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- 2021
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28. The therapeutic impact of entresto on protecting against cardiorenal syndrome-associated renal damage in rats on high protein diet
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Chih-Chao Yang, Yen-Ta Chen, Chih-Hung Chen, Yi-Chen Li, Pei-Lin Shao, Then-Hung Huang, Yi-Ling Chen, Cheuk-Kwan Sun, and Hon-Kan Yip
- Subjects
Cardiorenal syndrome ,Oxidative stress ,Apoptosis ,Mitochondrial damage ,Entresto ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Background: This study tested the hypothesis that Entresto could safely and effectively preserve heart and kidney function in rats with cardiorenal syndrome (CRS) induced by 5/6 nephrectomy and intra-peritoneal doxorubicin administration (accumulated dosage up to 7.5 mg/kg) together with daily high-protein-diet (HPD).Methods and Results: Adult male Sprague-Dawley rats (n = 24) were equally categorized into Group 1 (sham-operated control + HPD), Group 2 (CRS + HPD) and Group 3 [CRS + HPD + Entresto (100 mg/kg/day orally) since Day 14 after CRS induction] and euthanized by Day 63 after CRS induction. By Day 63, circulatory BUN and creatinine levels and ratios of urine protein to creatinine were significantly higher in Group 2 than those in Groups 1 and 3, and significantly higher in Group 3 than in Group 1, whereas left-ventricular ejection fraction and kidney weight showed an opposite pattern among all groups (all p
- Published
- 2019
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29. Long-Chain Fatty Acid Receptors Mediate Relaxation of the Porcine Lower Esophageal Sphincter
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Ching-Chung Tsai, Yi-Chen Li, Li-Ching Chang, Shu-Leei Tey, Kai-Jen Lin, and Shih-Che Huang
- Subjects
motility ,lower esophageal sphincter ,free fatty acid receptor ,FFA1 ,FFA4 ,Physiology ,QP1-981 - Abstract
Long-chain fatty acids activate the free fatty acid receptor 1 (FFA1) and FFA4. In the gastrointestinal system, FFA1 and FFA4 have been found in the pancreas and intestine. Fatty food and decreased lower esophageal sphincter (LES) motility are associated with gastroesophageal reflux disease. The effect of long-chain fatty acids on the esophageal motility is unknown. The purpose of this study is to investigate the effects of long-chain fatty acids on the porcine LES motility ex vivo using isometric transducers. In endothelin 1-precontracted porcine LES strips, the FFA1 selective agonists, fasiglifam, TUG424, and GW9508, caused marked relaxations in a concentration-dependent manner. The relative efficacies to elicit relaxation were GW9508 > TUG424 > fasiglifam in both clasp and sling strips. In contrast, the FFA4 specific agonists, TUG891 and GSK137647, produced mild relaxations. In addition, the endogenous FFA1 agonist DHA caused a mild relaxation whereas GW1100, an FFA1 antagonist, inhibited GW9508 induced relaxation of the porcine LES clasp and sling muscle. Both real-time PCR and immunohistochemistry revealed that FFA1 and FFA4 were expressed in the porcine LES. Real-time PCR analysis showed that the FFA4 expression was much lower than FFA1. Taken together, long-chain fatty acid receptor agonists elicit relaxation of the porcine LES. FFA1 might influence LES motility.
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- 2019
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30. Targeting HR Repair as a Synthetic Lethal Approach to Increase DNA Damage Sensitivity by a RAD52 Inhibitor in BRCA2-Deficient Cancer Cells
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Wei-Che Tseng, Chi-Yuan Chen, Ching-Yuh Chern, Chu-An Wang, Wen-Chih Lee, Ying-Chih Chi, Shu-Fang Cheng, Yi-Tsen Kuo, Ya-Chen Chiu, Shih-Ting Tseng, Pei-Ya Lin, Shou-Jhen Liou, Yi-Chen Li, and Chin-Chuan Chen
- Subjects
DNA repair ,curcumin ,RAD52 inhibitor ,synthetic lethal ,BRCA-deficient ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
BRCA mutation, one of the most common types of mutations in breast and ovarian cancer, has been suggested to be synthetically lethal with depletion of RAD52. Pharmacologically inhibiting RAD52 specifically eradicates BRCA-deficient cancer cells. In this study, we demonstrated that curcumin, a plant polyphenol, sensitizes BRCA2-deficient cells to CPT-11 by impairing RAD52 recombinase in MCF7 cells. More specifically, in MCF7-siBRCA2 cells, curcumin reduced homologous recombination, resulting in tumor growth suppression. Furthermore, a BRCA2-deficient cell line, Capan1, became resistant to CPT-11 when BRCA2 was reintroduced. In vivo, xenograft model studies showed that curcumin combined with CPT-11 reduced the growth of BRCA2-knockout MCF7 tumors but not MCF7 tumors. In conclusion, our data indicate that curcumin, which has RAD52 inhibitor activity, is a promising candidate for sensitizing BRCA2-deficient cells to DNA damage-based cancer therapies.
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- 2021
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31. Wind Farm Fault Detection by Monitoring Wind Speed in the Wake Region
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Minh-Quang Tran, Yi-Chen Li, Chen-Yang Lan, and Meng-Kun Liu
- Subjects
wind turbine fault detection ,feature selection ,wind energy dissipation model ,machine learning ,Technology - Abstract
A novel concept of wind farm fault detection by monitoring the wind speed in the wake region is proposed in this study. A wind energy dissipation model was coupled with a computational fluid dynamics solver to simulate the fluid field of a wind turbine array, and the wind velocity and direction in the simulation were exported for identifying wind turbine faults. The 3D steady Navier–Stokes equations were solved by using the cell center finite volume method with a second order upwind scheme and a k−ε turbulence model. In addition, the wind energy dissipation model, derived from energy balance and Betz’s law, was added to the Navier–Stokes equations’ source term. The simulation results indicate that the wind speed distribution in the wake region contains significant information regarding multiple wind turbine faults. A feature selection algorithm specifically designed for the analysis of wind flow was proposed to reduce the number of features. This algorithm proved to have better performance than fuzzy entropy measures and recursive feature elimination methods under a limited number of features. As a result, faults in the wind turbine array could be detected and identified by machine learning algorithms.
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- 2020
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32. Inhibition of growth and migration of oral and cervical cancer cells by citrus polyphenol
- Author
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Hsiao-Cheng Tsai, Yi-Chen Li, Sheng-Hao Hsu, Tai-Horng Young, and Min-Huey Chen
- Subjects
citrus polyphenol ,migration ,oral cancer cell ,proliferation ,Medicine (General) ,R5-920 - Abstract
It has been confirmed that polyphenolic compounds present in food have various pharmaceutical functions. The purpose of this study was to evaluate citrus polyphenol (CP) for dental applications. The culture medium with CP was developed to inhibit the proliferation of oral cancer cells. CP could be used as a supplemental compound for topical application for oral cancer patients. Methods: In this study, the metabolic activity and cell toxicity of CP (at concentrations of 1%, 0.1%, and 0.01%) for oral and cervical cancer cells were investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide and lactate dehydrogenase assays (n = 6). Furthermore, the effects of CP on motilities of oral and cervical cancer cells were also evaluated using a scratch assay model. Results: We found that the growth of Ca9-22 and HeLa cells on tissue culture polystyrene was greatly inhibited when 1% CP was added to the medium. In addition, significant differences (p
- Published
- 2016
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33. Citrus polyphenol for oral wound healing in oral ulcers and periodontal diseases
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Hsiao-Cheng Tsai, Yi-Chen Li, Tai-Horng Young, and Min-Huey Chen
- Subjects
citrus polyphenol ,proliferation ,wound healing ,Medicine (General) ,R5-920 - Abstract
Various polyphenolic compounds from plants have been confirmed to have different pharmaceutical functions. The purpose of this study was to evaluate citrus polyphenol (CP) for dental applications. A medium with CP was developed to improve oral wound healing. The CP could be used as a supplemental compound in mouthwash for periodontal diseases. Method: In this study, the metabolic activity and cell toxicity of CP (1%, 0.1%, and 0.01%) for fibroblasts were investigated by MTT and lactate dehydrogenase assays (n = 6). The effect of CP on motility of fibroblast was also evaluated via a wound healing model. Results: The growth of Hs68 cells on TCPS was greatly increased in the presence of 0.01% CP. In addition, the significant difference (p
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- 2016
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34. Novel microinjector for carrying bone substitutes for bone regeneration in periodontal diseases
- Author
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Hsiao-Cheng Tsai, Yi-Chen Li, Tai-Horng Young, and Min-Huey Chen
- Subjects
bone defect ,microinjector ,periodontal disease ,Medicine (General) ,R5-920 - Abstract
Traditionally, guide bone regeneration (GBR) was a widely used method for repairing bone lost from periodontal disease. There were some disadvantages associated with the GBR method, such as the need for a stable barrier membrane and a new creative cavity during the surgical process. To address these disadvantages, the purpose of this study was to evaluate a novel microinjector developed for dental applications. The microinjector was designed to carry bone graft substitutes to restore bone defects for bone regeneration in periodontal diseases. The device would be used to replace the GBR method. Methods: In this study, the injected force and ejected volume of substitutes (including air, water, and ethanol) were defined by Hooke's law (n = 3). The optimal particle size of bone graft substitutes was determined by measuring the recycle ratio of bone graft substitutes from the microinjector (n = 3). Furthermore, a novel agarose gel model was used to evaluate the feasibility of the microinjector. Results: The current study found that the injected force was less than 0.4 N for obtaining the ejected volume of approximately 2 mL, and when the particle size of tricalcium phosphate (TCP) was smaller than 0.5 mm, 80% TCP could be ejected from the microinjector. Furthermore, by using an agarose model to simulate the periodontal soft tissue, it was also found that bone graft substitutes could be easily injected into the gel. Conclusion: The results confirmed the feasibility of this novel microinjector for dental applications to carry bone graft substitutes for the restoration of bone defects of periodontal disease.
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- 2016
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35. Circulatory Rejuvenated EPCs Derived from PAOD Patients Treated by CD34+ Cells and Hyperbaric Oxygen Therapy Salvaged the Nude Mouse Limb against Critical Ischemia
- Author
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Yin-Chia Chen, Jiunn-Jye Sheu, John Y. Chiang, Pei-Lin Shao, Shun-Cheng Wu, Pei-Hsun Sung, Yi-Chen Li, Yi-Ling Chen, Tien-Hung Huang, Kuan-Hung Chen, and Hon-Kan Yip
- Subjects
critical limb ischemia ,endothelial progenitor cells ,nude mice ,angiogenesis ,hyperbaric oxygen therapy ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
This study tested whether circulatory endothelial progenitor cells (EPCs) derived from peripheral arterial occlusive disease (PAOD) patients after receiving combined autologous CD34+ cell and hyperbaric oxygen (HBO) therapy (defined as rejuvenated EPCs) would salvage nude mouse limbs against critical limb ischemia (CLI). Adult-male nude mice (n = 40) were equally categorized into group 1 (sham-operated control), group 2 (CLI), group 3 (CLI-EPCs (6 × 105) derived from PAOD patient’s circulatory blood prior to CD34+ cell and HBO treatment (EPCPr-T) by intramuscular injection at 3 h after CLI induction) and group 4 (CLI-EPCs (6 × 105) derived from PAOD patient’s circulatory blood after CD34+ cell and HBO treatment (EPCAf-T) by the identical injection method). By 2, 7 and 14 days after the CLI procedure, the ischemic to normal blood flow (INBF) ratio was highest in group 1, lowest in group 2 and significantly lower in group 4 than in group 3 (p < 0.0001). The protein levels of endothelial functional integrity (CD31/von Willebrand factor (vWF)/endothelial nitric-oxide synthase (eNOS)) expressed a similar pattern to that of INBF. In contrast, apoptotic/mitochondrial-damaged (mitochondrial-Bax/caspase-3/PARP/cytosolic-cytochrome-C) biomarkers and fibrosis (Smad3/TGF-ß) exhibited an opposite pattern, whereas the protein expressions of anti-fibrosis (Smad1/5 and BMP-2) and mitochondrial integrity (mitochondrial-cytochrome-C) showed an identical pattern of INBF (all p < 0.0001). The protein expressions of angiogenesis biomarkers (VEGF/SDF-1α/HIF-1α) were progressively increased from groups 1 to 3 (all p < 0.0010). The number of small vessels and endothelial cell surface markers (CD31+/vWF+) in the CLI area displayed an identical pattern of INBF (all p < 0.0001). CLI automatic amputation was higher in group 2 than in other groups (all p < 0.001). In conclusion, EPCs from HBO-C34+ cell therapy significantly restored the blood flow and salvaged the CLI in nude mice.
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- 2020
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36. De-Glycyrrhizinated Licorice Extract Attenuates High Glucose-Stimulated Renal Tubular Epithelial–Mesenchymal Transition via Suppressing the Notch2 Signaling Pathway
- Author
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Yung-Chien Hsu, Pey-Jium Chang, Chun-Wu Tung, Ya-Hsueh Shih, Wen-Chiu Ni, Yi-Chen Li, Takuhiro Uto, Yukihiro Shoyama, Cheng Ho, and Chun-Liang Lin
- Subjects
diabetic nephropathy ,renal fibrosis ,renal tubular epithelial cells ,emt ,notch2 ,licorice ,glycyrrhizin ,de-glycyrrhizinated licorice ,Cytology ,QH573-671 - Abstract
Tubulointerstitial fibrosis is a major pathological hallmark of diabetic nephropathy. Increasing evidence has shown that epithelial-to-mesenchymal transition (EMT) of renal proximal tubular cells plays a crucial role in tubulointerstitial fibrosis. Herein, we aimed to elucidate the detailed mechanism of EMT in renal tubular cells under high glucose (HG) conditions, and to investigate the potential of licorice, a medicinal herb, to inhibit HG-induced EMT. Our results showed that renal tubular epithelial cells (normal rat kidney cell clone 52E; NRK-52E) exposed to HG resulted in EMT induction characterized by increased fibronectin and α-SMA (alpha-smooth muscle actin) but decreased E-cadherin. Elevated levels of cleaved Notch2, MAML-1 (mastermind-like transcriptional coactivator 1), nicastrin, Jagged-1 and Delta-like 1 were also concomitantly detected in HG-cultured cells. Importantly, pharmacological inhibition, small interfering RNA (siRNA)-mediated depletion or overexpression of the key components of Notch2 signaling in NRK-52E cells supported that the activated Notch2 pathway is essential for tubular EMT. Moreover, we found that licorice extract (LE) with or without glycyrrhizin, one of bioactive components in licorice, effectively blocked HG-triggered EMT in NRK-52E cells, mainly through suppressing the Notch2 pathway. Our findings therefore suggest that Notch2-mediated renal tubular EMT could be a therapeutic target in diabetic nephropathy, and both LE and de-glycyrrhizinated LE could have therapeutic potential to attenuate renal tubular EMT and fibrosis.
- Published
- 2020
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37. Shock Wave Therapy Enhances Mitochondrial Delivery into Target Cells and Protects against Acute Respiratory Distress Syndrome
- Author
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Kun-Chen Lin, Christopher Glenn Wallace, Tsung-Cheng Yin, Pei-Hsun Sung, Kuan-Hung Chen, Hung-I Lu, Han-Tan Chai, Chih-Hung Chen, Yi-Ling Chen, Yi-Chen Li, Pei-Lin Shao, Mel S. Lee, Jiunn-Jye Sheu, and Hon-Kan Yip
- Subjects
Pathology ,RB1-214 - Abstract
This study tested the hypothesis that shock wave therapy (SW) enhances mitochondrial uptake into the lung epithelial and parenchymal cells to attenuate lung injury from acute respiratory distress syndrome (ARDS). ARDS was induced in rats through continuous inhalation of 100% oxygen for 48 h, while SW entailed application 0.15 mJ/mm2 for 200 impulses at 6 Hz per left/right lung field. In vitro and ex vivo studies showed that SW enhances mitochondrial uptake into lung epithelial and parenchyma cells (all p
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- 2018
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38. Dynamics Adaptive Safe Reinforcement Learning with a Misspecified Simulator.
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Ruiqi Xue, Ziqian Zhang, Lihe Li, Feng Chen 0042, Yi-Chen Li 0001, Yang Yu 0001, and Lei Yuan 0001
- Published
- 2024
- Full Text
- View/download PDF
39. A Driver Activity Dataset with Multiple RGB-D Cameras and mmWave Radars.
- Author
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Guan-Hua Li, Hsin-Che Chiang, Yi-Chen Li 0005, Shervin Shirmohammadi, and Cheng-Hsin Hsu
- Published
- 2024
- Full Text
- View/download PDF
40. Cost-aware Offline Safe Meta Reinforcement Learning with Robust In-Distribution Online Task Adaptation.
- Author
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Cong Guan, Ruiqi Xue, Ziqian Zhang, Lihe Li, Yi-Chen Li 0001, Lei Yuan 0001, and Yang Yu 0001
- Published
- 2024
- Full Text
- View/download PDF
41. Disentangling Policy from Offline Task Representation Learning via Adversarial Data Augmentation.
- Author
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Chengxing Jia, Fuxiang Zhang, Yi-Chen Li 0001, Chenxiao Gao, Xu-Hui Liu, Lei Yuan 0001, Zongzhang Zhang, and Yang Yu 0001
- Published
- 2024
- Full Text
- View/download PDF
42. Numerical Simulation of Turbulent Fluid Flow and Heat Transfer in a Ribbed Rotating Two-Pass Square Duct
- Author
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Tong-Miin Liou, Shih-Hui Chen, and Yi-Chen Li
- Subjects
ribbed rotating duct ,turbulent flow ,heat transfer ,two-layer model ,RANS. ,Engineering (General). Civil engineering (General) ,TA1-2040 - Abstract
The local turbulent fluid flow and heat transfer in a rotating two-pass square duct with 19 pairs of in-line 90∘ ribs have been investigated computationally. A Reynolds-averaged Navier-Stokes equation (RANS) with a two-layer k−ε turbulence model was solved. The in-line 90∘ ribs were arranged on the leading and trailing walls with rib height-to-hydraulic diameter ratio and pitch-to-height ratio of 0.136 and 10, respectively. The Reynolds number, based on duct hydraulic diameter and bulk mean velocity, was fixed at 1.0×104 whereas the rotational number varied from 0 to 0.2. Results are validated with previous measured velocity field and heat transfer coefficient distributions. The validation shows that the effect of rotation on the passage-averaged Nusselt number ratio can be predicted reasonably well; nevertheless, the transverse mean velocity and, in turn, the distribution of regional-averaged Nusselt number ratio are markedly underpredicted in the regions toward which the Coriolis force is directed. Further CFD studies are needed.
- Published
- 2005
- Full Text
- View/download PDF
43. DSG3 facilitates cancer cell growth and invasion through the DSG3-plakoglobin-TCF/LEF-Myc/cyclin D1/MMP signaling pathway.
- Author
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Yin-Ju Chen, Li-Yu Lee, Yin-Ka Chao, Joseph T Chang, Ya-Ching Lu, Hsiao-Fang Li, Ching-Chi Chiu, Yi-Chen Li, Yan-Liang Li, Jeng-Fong Chiou, and Ann-Joy Cheng
- Subjects
Medicine ,Science - Abstract
Desmoglein 3 (DSG3) is a component of the desmosome, which confers strong cell-cell adhesion. Previously, an oncogenic function of DSG3 has been found in head neck cancer (HNC). Here, we investigated how this molecule contributes to the malignant phenotype. Because DSG3 is associated with plakoglobin, we examined whether these phenotypic alterations were mediated through the plakoglobin molecule. Immunoprecipitation and immunofluorescence staining revealed that DSG3 silencing disrupted its interaction with plakoglobin and induced plakoglobin translocation from the cytoplasm to the nucleus. Knockdown of DSG3 significantly increased the interaction of plakoglobin with the transcriptional factor TCF and suppressed the TCF/LEF transcriptional activity. These effects further conferred to reduced expression of the TCF/LEF downstream target genes, including c-myc, cyclin D1, and MMP-7. Functional analyses showed that DSG3 silencing reduced cell growth and arrested cells at G0/G1 phase. Besides, cell migration and invasion abilities were also decreased. These cellular results were confirmed using tumor xenografts in mice, as DSG3 silencing led to the suppressed tumor growth, plakoglobin translocation and reduced expression of TCF/LEF target genes in tumors. Therefore, our study shows that the desmosomal protein DSG3 additionally functions to regulate malignant phenotypes via nuclear signaling. In conclusion, we found that DSG3 functions as an oncogene and facilitates cancer growth and invasion in HNC cells through the DSG3-plakoglobin-TCF/LEF pathway.
- Published
- 2013
- Full Text
- View/download PDF
44. Deep Demonstration Tracing: Learning Generalizable Imitator Policy for Runtime Imitation from a Single Demonstration.
- Author
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Xiong-Hui Chen, Junyin Ye, Hang Zhao, Yi-Chen Li 0001, XuHui Liu, Haoran Shi, Yu-Yan Xu, Zhihao Ye, Si-Hang Yang, Yang Yu 0001, Anqi Huang, Kai Xu 0004, and Zongzhang Zhang
- Published
- 2024
45. Debiased Offline Representation Learning for Fast Online Adaptation in Non-stationary Dynamics.
- Author
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Xinyu Zhang, Wenjie Qiu 0005, Yi-Chen Li 0001, Lei Yuan 0001, Chengxing Jia, Zongzhang Zhang, and Yang Yu 0001
- Published
- 2024
46. Q-Adapter: Training Your LLM Adapter as a Residual Q-Function.
- Author
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Yi-Chen Li 0001, Fuxiang Zhang, Wenjie Qiu 0005, Lei Yuan 0001, Chengxing Jia, Zongzhang Zhang, and Yang Yu 0001
- Published
- 2024
- Full Text
- View/download PDF
47. Improving Sample Efficiency of Reinforcement Learning with Background Knowledge from Large Language Models.
- Author
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Fuxiang Zhang, Junyou Li, Yi-Chen Li 0001, Zongzhang Zhang, Yang Yu 0001, and Deheng Ye
- Published
- 2024
- Full Text
- View/download PDF
48. BWArea Model: Learning World Model, Inverse Dynamics, and Policy for Controllable Language Generation.
- Author
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Chengxing Jia, Pengyuan Wang, Ziniu Li, Yi-Chen Li 0001, Zhilong Zhang, Nan Tang, and Yang Yu 0001
- Published
- 2024
- Full Text
- View/download PDF
49. Any-step Dynamics Model Improves Future Predictions for Online and Offline Reinforcement Learning.
- Author
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Haoxin Lin, Yu-Yan Xu, Yihao Sun, Zhilong Zhang, Yi-Chen Li 0001, Chengxing Jia, Junyin Ye, Jiaji Zhang, and Yang Yu 0001
- Published
- 2024
- Full Text
- View/download PDF
50. Policy Regularization with Dataset Constraint for Offline Reinforcement Learning.
- Author
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Yuhang Ran, Yi-Chen Li 0001, Fuxiang Zhang, Zongzhang Zhang, and Yang Yu 0001
- Published
- 2023
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