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140 results on '"Yin, Zhitong"'

3. Genetic Basis and Exploration of Major Expressed QTL qLA2-3 Underlying Leaf Angle in Maize.

Author

He, Yonghui, Wang, Chenxi, Hu, Xueyou, Han, Youle, Lu, Feng, Liu, Huanhuan, Zhang, Xuecai, and Yin, Zhitong

Subjects
LOCUS (Genetics), CULTIVARS, GERMPLASM, PHENOTYPIC plasticity, PLANT breeding
Abstract

Leaf angle (LA) is closely related to plant architecture, photosynthesis and density tolerance in maize. In the current study, we used a recombinant inbred line population constructed by two maize-inbred lines to detect quantitative trait loci (QTLs) controlling LA. Based on the average LA in three environments, 13 QTLs were detected, with the logarithm of odds ranging from 2.7 to 7.21, and the phenotypic variation explained by a single QTL ranged from 3.93% to 12.64%. A stable QTL, qLA2-3, on chromosome 2 was detected and was considered to be the major QTL controlling the LA. On the basis of verifying the genetic effect of qLA2-3, a fine map was used to narrow the candidate interval, and finally, the target segment was located at a physical distance of approximately 338.46 kb (B73 RefGen_v4 version), containing 16 genes. Re-sequencing and transcriptome results revealed that five candidate genes may be involved in the regulation of LA. The results enrich the information for molecular marker-assisted selection of maize LA and provide genetic resources for the breeding of dense planting varieties. [ABSTRACT FROM AUTHOR]

Published
2024
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41. Expression Quantitative Trait Loci Analysis of Two Genes Encoding Rubisco Activase in Soybean1[W][OA]

Author

Yin, Zhitong, Meng, Fanfan, Song, Haina, Wang, Xiaolin, Xu, Xiaoming, and Yu, Deyue

Subjects
DNA, Complementary, Base Sequence, DNA, Plant, fungi, Molecular Sequence Data, Quantitative Trait Loci, food and beverages, Chromosome Mapping, Sequence Analysis, DNA, Genes, Plant, Recombinant Proteins, Enzyme Activation, Isoenzymes, Gene Expression Regulation, Plant, Amino Acid Sequence, Soybeans, Cloning, Molecular, Photosynthesis, Sequence Alignment, Bioenergetics and Photosynthesis, Phylogeny, Molecular Chaperones, Plant Proteins
Abstract

Rubisco activase (RCA) catalyzes the activation of Rubisco in vivo and plays a crucial role in photosynthesis. However, until now, little was known about the molecular genetics of RCA in soybean (Glycine max), one of the most important legume crops. Here, we cloned and characterized two genes encoding the longer alpha -isoform and the shorter beta -isoform of soybean RCA (GmRCA alpha and GmRCA beta, respectively). The two corresponding cDNAs are divergent in both the translated and 3 ' untranslated regions. Analysis of genomic DNA sequences suggested that the corresponding mRNAs are transcripts of two different genes and not the products of a single alternatively splicing pre-mRNA. Two additional possible alpha -form RCA-encoding genes, GmRCA03 and GmRCA14, and one additional beta -form RCA-encoding gene, GmRCA11, were also isolated. To examine the function and modulation of RCA genes in soybean, we determined the expression levels of GmRCA alpha and GmRCA beta, Rubisco initial activity, photosynthetic rate, and seed yield in 184 soybean recombinant inbred lines. Correlation of gene expression levels with three other traits indicates that RCA genes could play an important role in regulating soybean photosynthetic capacity and seed yield. Expression quantitative trait loci mapping revealed four trans-expression quantitative trait loci for GmRCA alpha and GmRCA beta. These results could provide a new approach for the modulation of RCA genes to improve photosynthetic rate and plant growth in soybean and other plants.

Published
2009

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