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A polyacrylonitrile (PAN)-based immobilized metal-ion affinity membrane (IMAM) was prepared with a high capacity for protein adsorption. PAN was selected as the substrate due to its excellent thermal and chemical stability. The cyano groups on the PAN membrane were substituted with carboxyl groups, followed by reactions with ethylenediamine (EDA) and ethylene glycol diglycidyl ether (EGDGE) to produce the terminal epoxy groups. The chelating agent iminodiacetic acid (IDA) was then bound to the modified PAN membrane and further chelated with copper ions. The immobilized copper ion amount of membrane was analyzed to obtain the optimal reaction conditions, which were 60 °C/3 h for EDA coupling and 60 °C/4 h for EGDGE grafting. Furthermore, under the use of minor IDA and copper ion concentrations, the immobilized copper ion capacity of the IMAM was 4.8 μmol/cm2 (253.4 µmol/mL, or 1.47 μmol/mg). At a neutral pH, the cationic lysozyme exhibited a large adsorption capacity with the IMAM (1.96 μmol/mL), which was most likely multilayer binding, whereas the adsorption capacity for bovine serum albumin (BSA) and histidine-tagged green fluorescent protein (GFP-His6) was 0.053 μmol/mL and 0.135 μmol/mL, respectively, with a monolayer adsorption arrangement. The protein desorption efficiency was greater than 95%, implying that the prepared IMAM could be reused for protein adsorption.

We aimed to investigate whether peripheral low-dose lipopolysaccharide (LPS) induces the breakdown of the blood–brain barrier (BBB) and/or the activation of toll-like receptor 4 (TLR4) in the neonatal rat brain. Neonatal rats received intraperitoneal injections of low-dose LPS (0.3 mg/kg∙bw), and the BBB compromise was detected by Evans Blue extravasation and electron microscopy. Meanwhile, TLR4, adaptin myeloid differentiation factor 88 (MyD88), nuclear transcription factor kappa-B (NF-κB) p50 and tumor necrosis factor alpha (TNFα) in the neonatal rat brain were determined by quantitative real-time polymerase chain reaction (PCR) and Western Blot. Immunohistochemistry was used to determine the distribution and activation of microglia in the brain after LPS administration. It was demonstrated that Evans Blue extravasation was not observed in the brain parenchyma, and that tight junctions of cerebral endothelial cells remained intact after systemic injections of LPS in neonatal rats. Although intracerebroventricular injections of LPS activated microglia and up-regulated the expression of TLR4, MyD88, NF-κB p50 and TNFα in the neonatal rat brain, systemic LPS did not induce these responses. These findings indicate that while the neonatal rat brain responds to the direct intra-cerebral administration of LPS through robust TLR4 activation, systemic low-dose LPS does not induce the innate immune reaction or compromise the BBB in neonatal rats.

WaveOne Gold, ProTaper Gold, Reciproc Blue, ProTaper Next, WaveOne and ProTaper files were selected to compare the phase transformation behaviors and mechanical properties of nickel-titanium (NiTi) rotary files after gold heat and blue heat treatments. The reverse transformation finishing point temperatures of WaveOne Gold, ProTaper Gold, ProTaper Next and WaveOne were higher than those of the other two instruments investigated. At a deflection of 0.5 mm, the loads were significantly varied except for ProTaper Next and ProTaper Gold. At a deflection of 3.0 mm, the loads of Reciproc Blue and WaveOne Gold were significantly varied compared to WaveOne and ProTaper. Cycles to failure were reduced in the order of WaveOne Gold, Reciproc Blue, ProTaper Gold, ProTaper Next, WaveOne and ProTaper. NiTi instruments after gold heat and blue heat treatments exhibited significantly higher bending properties and cyclic fatigue resistances, representing an improved performance over traditional and M-wire instruments. [ABSTRACT FROM AUTHOR]