111 results on '"Ying-Li Liu"'
Search Results
2. Fructose promotes ampicillin killing of antibiotic-resistant Streptococcus agalactiae
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Xuan-Wei Chen, Jia-Han Wu, Ying-Li Liu, Hetron Mweemba Munang’andu, and Bo Peng
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Streptococcus agalactiae ,ampicillin ,fructose ,glycolysis ,metabolism ,Infectious and parasitic diseases ,RC109-216 - Abstract
ABSTRACTStreptococcus agalactiae (GBS) is an important pathogenic bacteria that infected both aquatic animals and human beings, causing huge economic loss. The increasing cases of antibiotic-resistant GBS impose challenges to treat such infection by antibiotics. Thus, it is highly demanded for the approach to tackle antibiotic resistance in GBS. In this study, we adopt a metabolomic approach to identify the metabolic signature of ampicillin-resistant GBS (AR-GBS) that ampicillin is the routine choice to treat infection by GBS. We find glycolysis is significantly repressed in AR-GBS, and fructose is the crucial biomarker. Exogenous fructose not only reverses ampicillin resistance in AR-GBS but also in clinic isolates including methicillin-resistant Staphylococcus aureus (MRSA) and NDM-1 expressing Escherichia coli. The synergistic effect is confirmed in a zebrafish infection model. Furthermore, we demonstrate that the potentiation by fructose is dependent on glycolysis that enhances ampicillin uptake and the expression of penicillin-binding proteins, the ampicillin target. Our study demonstrates a novel approach to combat antibiotic resistance in GBS.
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- 2023
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3. A method for analyzing programmed cell death in xylem development by flow cytometry
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Ying-Li Liu, Ying-Hua Guo, Xue-Qin Song, Meng-Xuan Hu, and Shu-Tang Zhao
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PCD ,xylem development ,FACS ,gene expression ,woody plants ,Plant culture ,SB1-1110 - Abstract
Programmed cell death (PCD) is a genetically regulated developmental process leading to the death of specific types of plant cells, which plays important roles in plant development and growth such as wood formation. However, an efficient method needs to be established to study PCD in woody plants. Flow cytometry is widely utilized to evaluate apoptosis in mammalian cells, while it is rarely used to detect PCD in plants, especially in woody plants. Here, we reported that the xylem cell protoplasts from poplar stem were stained with a combination of fluorescein annexin V-FITC and propidium iodide (PI) and then sorted by flow cytometry. As expected, living cells (annexin V-FITC negative/PI negative), early PCD cells (annexin V-FITC positive/PI negative), and late PCD cells (annexin V-FITC positive/PI positive) could be finely distinguished through this method and then subjected for quantitative analysis. The expression of cell-type- and developmental stages-specific marker genes was consistent with the cell morphological observation. Therefore, the newly developed fluorescence-activated cell sorting (FACS) method can be used to study PCD in woody plants, which will be beneficial for studying the molecular mechanisms of wood formation.
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- 2023
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4. The Impact of Hypertension Definition Based on Two-visit Strategy on Estimate of Hypertension Burden: Results From the China Health and Nutrition Survey 1989–2011
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Ying-Li Liu, Ying-Jun Mi, Bing Zhang, Hui-Jun Wang, Jie Yu, Xing-Bing Pan, Chao Wang, and Qing-Bao Tian
- Subjects
hypertension definition ,two-visit strategy ,hypertension burden ,chinese adults ,Medicine (General) ,R5-920 - Abstract
Background: The diagnosis of hypertension should be based on the mean of two or more properly measured BP readings on each of two visits for clinical practice, but a one-visit strategy was applied in most epidemiological surveys. The impact of hypertension definition based on two visits on estimates of hypertension burden is unknown. This study aims to assess the impact of hypertension diagnosis based on a two-visit strategy for estimating hypertension burden in China. Methods: The one-visit and two-visit strategies were applied to investigate the incidence of hypertension in a cohort study based on the China Health and Nutrition Survey (CHNS) 1989–2011. Additionally the prevalence of hypertension was investigated in a cross-sectional study based on the CHNS 2006–2009/2011 and the hypertension burden in China was estimated with data from the 2012–2015 China hypertension survey. Results: Overall, the age-adjusted incidence of hypertension based on the two-visit strategy (1.82%; 95% confidence interval [CI], 1.74–1.90%) was 62.1% lower than estimation based on the one-visit strategy (4.80%; 95% CI, 4.68–4.93%). Similar results were found in the prevalence of hypertension (one-visit: 18.13% [95% CI, 17.34–18.92%]; two-visit: 9.47% [95% CI, 8.87–10.07%]). When the two-visit strategy was applied to the 2012–2015 China hypertension survey, the hypertension burden was predicted to be overestimated by 25.5–47.8% (based on JNC 7) and 23.5–48.2% (based on the 2017 ACC/AHA). Conclusion: The hypertension burden would decrease from 244.5 million persons to 127.5–182.3 million persons in China if the two-visit strategy was applied.
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- 2021
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5. Plain Water Intake and Association With the Risk of Overweight in the Chinese Adult Population: China Health and Nutrition Survey 2006–2011
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Xing-Bing Pan, Hui-Jun Wang, Bing Zhang, Ying-Li Liu, Su-Fen Qi, and Qing-Bao Tian
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plain water ,overweight ,obesity ,dose–response ,chns ,Medicine (General) ,R5-920 - Abstract
Background: The prevalence of overweight is increasing dramatically worldwide. The aim of our study was to investigate the association of plain water intake (PWI) with the risk of new-onset overweight risk among Chinese adults. Methods: A total of 3,200 adults aged 18–65 who were free of overweight at baseline were enrolled from China Health and Nutrition Survey (CHNS) cohort study in 2006–2011. The risk of new-onset overweight with different amounts of PWI per day was analyzed in this 5-year cohort. A multiple logistic regression model was used to assess the association of PWI and the risk of new-onset overweight and adjust for potential confounders. Moreover, dose-response models were developed to estimate the linear relationship. Results: During 5 years of follow-up, 1,018 incident cases were identified. Our analysis indicated an inverse association of more than 4 cups of PWI per day and the risk of new-onset overweight among normal weight individuals. Compared with participants who drank 2 to 3 cups PWI, the adjusted odds ratios (OR) of overweight were 0.741 (95% confidence interval [CI], 0.599–0.916) in participants who drank 4 to 5 cups PWI, and 0.547 (95% CI, 0.435–0.687) in participants who drank more than 6 cups PWI. The dose-response analysis showed that every cup of PWI was associated with a 6.5% and 8.4% decrease in the risk of new-onset overweight among men and women, respectively. The interactions of PWI and covariates on the risk of overweight were not found. Conclusion: Drinking more than 4 cups (≈1 liter) per day of plain water is associated with decrease in the risk of new-onset overweight among normal-weight individuals.
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- 2020
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6. Identification of the Genetic Requirements for Zinc Tolerance and Toxicity in Saccharomyces cerevisiae
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Yun-ying Zhao, Chun-lei Cao, Ying-li Liu, Jing Wang, Jie Li, Shi-yun Li, and Yu Deng
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saccharomyces cerevisiae ,zinc toxicity ,genetic screening ,genomics ,reactive oxygen species (ros) ,Genetics ,QH426-470 - Published
- 2020
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7. A Small Guanosine Triphosphate Binding Protein PagRabE1b Promotes Xylem Development in Poplar
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Ying-Li Liu, Li-Juan Wang, Yu Li, Ying-Hua Guo, Yuan Cao, and Shu-Tang Zhao
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poplar ,small gtp binding protein ,PagRabE1b ,cell wall ,wood formation ,Plant culture ,SB1-1110 - Abstract
Rab GTPases are the subfamily of the small guanosine triphosphate (GTP)-binding proteins which participated in the regulation of various biological processes. Recent studies have found that plant Rabs play some specific functions. However, the functions of Rabs in xylem development in trees remain unclear. In this study, functional identification of PagRabE1b in Populus was performed. Quantitative reverse transcription PCR (qRT-PCR) results showed that PagRabE1b was highly accumulated in stems, especially in phloem and xylem tissues. Overexpression of PagRabE1b in poplar enhanced programmed cell death (PCD) and increased the growth rate and the secondary cell wall (SCW) thickness. Quantitative analysis of monosaccharide content showed that various monosaccharides were significantly increased in secondary xylem tissues of the overexpressed lines. Flow cytometry analysis revealed that the number of apoptotic cells in PagRabE1b-OE lines is more than a wild type (WT), which indicated that PagRabE1b may play an important role in PCD. Further studies showed that overexpression of PagRabE1b increased the expression level of genes involved in SCW biosynthesis, PCD, and autophagy. Collectively, the results suggest that PagRabE1b plays a positive role in promoting the xylem development of poplar.
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- 2021
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8. Astragaloside IV Improves High-Fat Diet–Induced Hepatic Steatosis in Nonalcoholic Fatty Liver Disease Rats by Regulating Inflammatory Factors Level via TLR4/NF-κB Signaling Pathway
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Ying-Li Liu, Qiu-Zan Zhang, Yan-Rong Wang, Li-Na Fu, Jing-Shu Han, Jing Zhang, and Bang-Mao Wang
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astragaloside IV ,non-alcoholic fatty liver disease ,toll like receptor 4 ,nuclear factor-kappa B ,myeloid differentiation factor 88 ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Objective: Astragaloside IV (AS-IV) is the primary bioactive component purified from Astragalus membranaceus which is one of the traditional Chinese medicines. Research studies found that AS-IV has significant pharmacological effects on focal cerebral ischemia/reperfusion, cardiovascular disease, pulmonary disease, liver cirrhosis, and diabetic nephropathy, but little is known about the effects of AS-IV on nonalcoholic fatty liver disease (NAFLD). In this study, we investigated whether AS-IV has beneficial effects on NAFLD in rats and its potential mechanisms.Methods: Male SD rats were fed with high-fat diet (HFD) for 12 weeks to establish NAFLD rat model, and then, the rats were divided into five groups. The control group rats were fed with normal diet for 12 weeks and then were given normal saline (1.0 ml kg−1 day−1) by intragastric administration for 4 weeks. The model group rats were fed with HFD for 12 weeks and then were given normal saline (1.0 ml kg−1 day−1) by intragastric administration for 4 weeks. The AS-IV-L, AS-IV-M, and AS-IV-H groups were treated with 20, 40, and 80 mg kg−1 day−1 of AS-IV by intragastric administration for 4 weeks and given HFD diet. Then, we detected serum transaminase (ALT, AST), blood lipid (TG, TC), inflammatory cytokines (IL-6, IL-8 and TNF-α), liver histology(NAFLD activity score), TLR4/MyD88 signaling pathway in liver tissue.Results: We found AS-IV significantly reduced serum levels of AST, ALT, TG, TNF-α, IL-6, and IL-8 in NAFLD rats and downregulate the expression of TLR4 mRNA, MyD88 mRNA, NF-κB mRNA, and proteins in liver tissue. Moreover, AS-IV could significantly reduce the NAFLD activity score of NAFLD rat liver.Conclusion: In this study, we demonstrated that AS-IV have a protective effect on NAFLD by inhibiting TNF-α, IL-6 and IL-8 levels and down-regulating TLR4, MyD88 and NF-κB expression in rat liver tissues.
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- 2021
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9. Cordyceps militaris Immunomodulatory Protein Promotes the Phagocytic Ability of Macrophages through the TLR4-NF-κB Pathway
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Hong-Bo Fan, Yuan Zou, Qing Han, Qian-Wang Zheng, Ying-Li Liu, Li-Qiong Guo, and Jun-Fang Lin
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Cordyceps militaris ,immunomodulatory protein ,macrophage ,phagocytosis ,immunity ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Enhancing the phagocytosis of immune cells with medicines provides benefits to the physiological balance by removing foreign pathogens and apoptotic cells. The fungal immunomodulatory protein (FIP) possessing various immunopotentiation functions may be a good candidate for such drugs. However, the effect and mechanism of FIP on the phagocytic activity is limitedly investigated. Therefore, the present study determined effects of Cordyceps militaris immunomodulatory protein (CMIMP), a novel FIP reported to induce cytokines secretion, on the phagocytosis using three different types of models, including microsphere, Escherichia Coli and Candida albicans. CMIMP not only significantly improved the phagocytic ability (p < 0.05), but also enhanced the bactericidal activity (p < 0.05). Meanwhile, the cell size, especially the cytoplasm size, was markedly increased by CMIMP (p < 0.01), accompanied by an increase in the F-actin expression (p < 0.001). Further experiments displayed that CMIMP-induced phagocytosis, cell size and F-actin expression were alleviated by the specific inhibitor of TLR4 (p < 0.05). Similar results were observed in the treatment with the inhibitor of the NF-κB pathway (p < 0.05). In conclusion, it could be speculated that CMIMP promoted the phagocytic ability of macrophages through increasing F-actin expression and cell size in a TLR4-NF-κB pathway dependent way.
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- 2021
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10. A Case of Podocytic Infolding Glomerulopathy with Primary Sjögren's Syndrome and Hashimoto's Thyroiditis
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Jun-Yan Fang, A-Hui Song, Bo Shen, and Ying-Li Liu
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Medicine - Published
- 2018
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11. Secreted in a Type III Secretion System-Dependent Manner, EsaH and EscE Are the Cochaperones of the T3SS Needle Protein EsaG of Edwardsiella piscicida
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Zhi Xiong Zeng, Lu Yi Liu, Shui Bing Xiao, Jin Fang Lu, Ying Li Liu, Jing Li, Yuan Ze Zhou, Li Jing Liao, Duan You Li, Ying Zhou, Pin Nie, and Hai Xia Xie
- Subjects
cochaperone ,secretion ,T3SS needle protein ,Edwardsiella piscicida ,Microbiology ,QR1-502 - Abstract
ABSTRACT The intracellular EscE protein tightly controls the secretion of the type III secretion system (T3SS) middle and late substrates in Edwardsiella piscicida. However, the regulation of secretion by EscE is incompletely understood. In this work, we reveal that EscE interacts with EsaH and EsaG. The crystal structures of the EscE-EsaH complex and EscE-EsaG-EsaH complex were resolved at resolutions of 1.4 Å and 1.8 Å, respectively. EscE and EsaH form a hydrophobic groove to engulf the C-terminal region of EsaG (56 to 73 amino acids [aa]), serving as the cochaperones of T3SS needle protein EsaG in E. piscicida. V61, K62, M64, and M65 of EsaG play a pivotal role in maintaining the conformation of the ternary complex of EscE-EsaG-EsaH, thereby maintaining the stability of EsaG. An in vivo experiment revealed that EscE and EsaH stabilize each other, and both of them stabilize EsaG. Meanwhile, either EscE or EsaH can be secreted through the T3SS. The secondary structure of EsaH lacks the fourth and fifth α helices presented in its homologs PscG, YscG, and AscG. Insertion of the α4 and α5 helices of PscG or swapping the N-terminal 25 aa of PscG with those of EsaH starkly decreases the protein level of the chimeric EsaH, resulting in instability of EsaG and deactivation of the T3SS. To the best of our knowledge, these data represent the first reported structure of the T3SS needle complex of pathogens from Enterobacteriaceae and the first evidence for the secretion of T3SS needle chaperones. IMPORTANCE Edwardsiella piscicida causes severe hemorrhagic septicemia in fish. Inactivation of the type III secretion system (T3SS) increases its 50% lethal dose (LD50) by ~10 times. The secretion of T3SS middle and late substrates in E. piscicida is tightly controlled by the intracellular steady-state protein level of EscE, but the mechanism is incompletely understood. In this study, EscE was found to interact with and stabilize EsaH in E. piscicida. The EscE-EsaH complex is structurally analogous to T3SS needle chaperones. Further study revealed that EscE and EsaH form a hydrophobic groove to engulf the C-terminal region of EsaG, serving as the cochaperones stabilizing the T3SS needle protein EsaG. Interestingly, both EscE and EsaH are secreted. Our study reveals that the EscE-EsaH complex controls T3SS protein secretion by stabilizing EsaG, whose secretion in turn leads to the secretion of the middle and late T3SS substrates.
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- 2022
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12. PagERF81 regulates lignin biosynthesis and xylem cell differentiation in poplar
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Xin‐Wei Zhao, Qiao Wang, Dian Wang, Wei Guo, Meng‐Xuan Hu, Ying‐Li Liu, Gong‐Ke Zhou, Guo‐Hua Chai, Shu‐Tang Zhao, and Meng‐Zhu Lu
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Plant Science ,Biochemistry ,General Biochemistry, Genetics and Molecular Biology - Published
- 2023
13. Fast Conversion of Three‐Particle Dicke States to Four‐Particle Dicke States with Rydberg Superatoms
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Ying‐Li Liu, Yan‐Qiang Ji, Xue Han, Wen‐Xue Cui, Shou Zhang, and Hong‐Fu Wang
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Nuclear and High Energy Physics ,Computational Theory and Mathematics ,Statistical and Nonlinear Physics ,Electrical and Electronic Engineering ,Condensed Matter Physics ,Mathematical Physics ,Electronic, Optical and Magnetic Materials - Published
- 2023
14. On improving the hygroscopic stability of palmatine chloride with crystalline palmatine sulfosalicyate pharmaceutical salt
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Yu-Nan Zhang, Yu Duan, Li-Xin Liu, L. Chang, Yan-Ru Feng, Li-Li Wu, Lei Zhang, Yun-Jie Zhang, D.-Y. Zou, Ying-Li Liu, and Xin Su
- Abstract
With the aim to improve the hygroscopic stability of palmatine chloride (PMTCl), an unreported single crystal of PMT—SSA (C21H20NO4·C7H5O6S·H2O) is prepared by the solvent evaporation method and characterized. A comprehensive analysis of the structure and Hirshfeld surface reveals that π—π stacking plays a crucial role in accumulating PMT—SSA. As expected, the hygroscopic stability of PMT—SSA is significantly improved compared with that of PMTCl. This may be due to the presence of π—π stacking in the structure of PMT—SSA which improves the hygroscopic stability. The present work inaugurates an avenue for improving the hygroscopic stability of active pharmaceutical ingredients and provides a novel crystalline product with a bright commercial prospect. Unfortunately, the maximum solubility of PMT—SSA is lower than that of PMTCl. How to improve its solubility will be explored in subsequent studies. However, it is surprising that PMT—SSA shows advantages with regard to antibacterial properties in comparison to PMTCl.
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- 2022
15. The Impact of Hypertension Definition Based on Two-visit Strategy on Estimate of Hypertension Burden: Results From the China Health and Nutrition Survey 1989–2011
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Huijun Wang, Ying-Li Liu, Chao Wang, Ying-Jun Mi, Xing-Bing Pan, Jie Yu, Bing Zhang, and Qing-Bao Tian
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Adult ,Male ,medicine.medical_specialty ,China ,Adolescent ,Epidemiology ,Office Visits ,two-visit strategy ,030209 endocrinology & metabolism ,Blood Pressure ,Cohort Studies ,03 medical and health sciences ,0302 clinical medicine ,chinese adults ,Environmental health ,Cardiovascular Disease ,medicine ,Prevalence ,Nutrition survey ,Humans ,030212 general & internal medicine ,hypertension definition ,Hypertension diagnosis ,Aged ,Estimation ,lcsh:R5-920 ,business.industry ,Incidence (epidemiology) ,Incidence ,Blood Pressure Determination ,General Medicine ,hypertension burden ,Middle Aged ,Nutrition Surveys ,Health Surveys ,Confidence interval ,Cross-Sectional Studies ,Hypertension ,Original Article ,Female ,business ,lcsh:Medicine (General) ,Cohort study - Abstract
Background The diagnosis of hypertension should be based on the mean of two or more properly measured BP readings on each of two visits for clinical practice, but a one-visit strategy was applied in most epidemiological surveys. The impact of hypertension definition based on two visits on estimates of hypertension burden is unknown. This study aims to assess the impact of hypertension diagnosis based on a two-visit strategy for estimating hypertension burden in China. Methods The one-visit and two-visit strategies were applied to investigate the incidence of hypertension in a cohort study based on the China Health and Nutrition Survey (CHNS) 1989-2011. Additionally the prevalence of hypertension was investigated in a cross-sectional study based on the CHNS 2006-2009/2011 and the hypertension burden in China was estimated with data from the 2012-2015 China hypertension survey. Results Overall, the age-adjusted incidence of hypertension based on the two-visit strategy (1.82%; 95% confidence interval [CI], 1.74-1.90%) was 62.1% lower than estimation based on the one-visit strategy (4.80%; 95% CI, 4.68-4.93%). Similar results were found in the prevalence of hypertension (one-visit: 18.13% [95% CI, 17.34-18.92%]; two-visit: 9.47% [95% CI, 8.87-10.07%]). When the two-visit strategy was applied to the 2012-2015 China hypertension survey, the hypertension burden was predicted to be overestimated by 25.5-47.8% (based on JNC 7) and 23.5-48.2% (based on the 2017 ACC/AHA). Conclusion The hypertension burden would decrease from 244.5 million persons to 127.5-182.3 million persons in China if the two-visit strategy was applied.
- Published
- 2021
16. Robot-assisted versus stereotactic frame-based stereoelectroencephalography in medically refractory epilepsy
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Linxia Sang, Wenling Li, Jie Zheng, Di Zhang, Tao Xie, Ying-Li Liu, and Xuehua Cui
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Frame based ,Drug Resistant Epilepsy ,medicine.medical_specialty ,Multiple linear regression model ,050105 experimental psychology ,Stereoelectroencephalography ,03 medical and health sciences ,Epilepsy ,0302 clinical medicine ,Physiology (medical) ,Humans ,Medicine ,0501 psychology and cognitive sciences ,Epilepsy surgery ,In patient ,Retrospective Studies ,Retrospective review ,business.industry ,05 social sciences ,Electroencephalography ,Robotics ,General Medicine ,medicine.disease ,Electrodes, Implanted ,Neurology ,Refractory epilepsy ,Neurology (clinical) ,Radiology ,business ,030217 neurology & neurosurgery - Abstract
To explore the difference between robot assisted (RA) and stereotactic frame based (SF) stereoelectroencephalography (SEEG) in patients with medically refractory epilepsy.We undertook a retrospective review of 33 SEEG cases at our center, of which 14 were SF performed from March to October 2018 and 19 were RA performed from November 2018 to December 2019. Detailed review of medical histories and operative records as well as imaging and trajectory plans was carried out for each patient, and the results related to each technique compared. A multiple linear regression model was used to test for variables that significantly influenced placement error.Compared to the SF group, the RA group had a higher mean number of electrodes per patient (10.7 ± 2.8 versus 6.4 ± 0.8, P 0.0001) and a significantly shorter mean operative time (127.3 ± 40.7 versus 152.7 ± 13.6 min, P = 0.033). For the RA group, the intracranial implantation length was positively correlated with target point error (p = 0.000), depth error (p = 0.043), and two-dimensional (2D) radial error (p = 0.041). Conversely, skull thickness was negatively correlated with the TP error (p = 0.004), depth error (p = 0.037) and 2D radial error (p = 0.000). We also analyzed the mean entry point, target point, depth and 2D radial errors, the complication rates, and the results of epileptogenic zone (EZ) localization and Engel class. The results showed no difference in these aspects between the SF group and the RA group.This study suggests that, compared to stereotactic frame based SEEG, robot assisted SEEG is significantly more efficient and comparable in safety and effectiveness.
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- 2021
17. <scp>TMT‐MS</scp> / <scp>MS</scp> proteomic analysis of the carbohydrate‐active enzymes in the fruiting body of Pleurotus tuoliensis during storage
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Ying-Li Liu, Qianwang Zheng, Li-Qiong Guo, Jun-Fang Lin, Si-Qiang Ye, Rui‐Rong Li, and Yuan Zou
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Proteomics ,Autolysis (biology) ,Glycoside Hydrolases ,030309 nutrition & dietetics ,Biology ,Pleurotus ,Polysaccharide ,Tandem mass tag ,Lignin ,Fungal Proteins ,Cell wall ,03 medical and health sciences ,0404 agricultural biotechnology ,Tandem Mass Spectrometry ,Fruiting Bodies, Fungal ,Food science ,Amylase ,Glucan ,chemistry.chemical_classification ,0303 health sciences ,Nutrition and Dietetics ,beta-Glucosidase ,Chitinases ,04 agricultural and veterinary sciences ,biology.organism_classification ,040401 food science ,chemistry ,Xylanase ,biology.protein ,Agronomy and Crop Science ,Food Science ,Biotechnology - Abstract
BACKGROUND The fruiting body of Pleurotus tuoliensis deteriorates rapidly after harvest, causing a decline in its commercial value and a great reduction in its shelf life. According to the present research, carbohydrate-active enzymes (CAZymes) may cause the softening, liquefaction and autolysis of mature mushrooms after harvest. To further understand the in vivo molecular mechanism of CAZymes affecting the postharvest quality of P. tuoliensis fruiting bodies, a tandem mass tags labelling combined liquid chromatography-tandem mass spectrometry (TMT-MS/MS) proteomic analysis was performed on P. tuoliensis fruiting bodies during storage at 25 °C. RESULTS A total of 4737 proteins were identified, which had at least one unique peptide and had a confidence level above 95%. Consequently, 1307 differentially expressed proteins (DEPs) were recruited using the criteria of abundance fold change (FC) >1.5 or
- Published
- 2020
18. Plain Water Intake and Association With the Risk of Overweight in the Chinese Adult Population: China Health and Nutrition Survey 2006–2011
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Su-Fen Qi, Ying-Li Liu, Huijun Wang, Xing-Bing Pan, Bing Zhang, and Qing-Bao Tian
- Subjects
Adult ,Male ,obesity ,China ,CHNS ,Adolescent ,Epidemiology ,Drinking ,030209 endocrinology & metabolism ,Overweight ,Risk Assessment ,Cohort Studies ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,plain water ,Medicine ,Humans ,030212 general & internal medicine ,Nutrition ,Aged ,lcsh:R5-920 ,business.industry ,Confounding ,Liter ,General Medicine ,Odds ratio ,Middle Aged ,medicine.disease ,Nutrition Surveys ,Obesity ,Confidence interval ,Cohort ,Original Article ,Female ,medicine.symptom ,dose–response ,business ,lcsh:Medicine (General) ,Demography ,Cohort study - Abstract
Background The prevalence of overweight is increasing dramatically worldwide. The aim of our study was to investigate the association of plain water intake (PWI) with the risk of new-onset overweight risk among Chinese adults. Methods A total of 3,200 adults aged 18-65 who were free of overweight at baseline were enrolled from China Health and Nutrition Survey (CHNS) cohort study in 2006-2011. The risk of new-onset overweight with different amounts of PWI per day was analyzed in this 5-year cohort. A multiple logistic regression model was used to assess the association of PWI and the risk of new-onset overweight and adjust for potential confounders. Moreover, dose-response models were developed to estimate the linear relationship. Results During 5 years of follow-up, 1,018 incident cases were identified. Our analysis indicated an inverse association of more than 4 cups of PWI per day and the risk of new-onset overweight among normal weight individuals. Compared with participants who drank 2 to 3 cups PWI, the adjusted odds ratios (OR) of overweight were 0.741 (95% confidence interval [CI], 0.599-0.916) in participants who drank 4 to 5 cups PWI, and 0.547 (95% CI, 0.435-0.687) in participants who drank more than 6 cups PWI. The dose-response analysis showed that every cup of PWI was associated with a 6.5% and 8.4% decrease in the risk of new-onset overweight among men and women, respectively. The interactions of PWI and covariates on the risk of overweight were not found. Conclusion Drinking more than 4 cups (≈1 liter) per day of plain water is associated with decrease in the risk of new-onset overweight among normal-weight individuals.
- Published
- 2020
19. The role of senescence-associated gene101 (PagSAG101a) in the regulation of secondary xylem formation in poplar
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Dian Wang, Mengzhu Lu, Cheng Jiang, Guo-Hua Chai, Hui He, Shuang-Shuang Wen, Xueqin Song, Ying-Li Liu, and Shutang Zhao
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Zinc finger ,Cambium ,Cell division ,fungi ,food and beverages ,Xylem ,Plant Science ,Biology ,biology.organism_classification ,Plants, Genetically Modified ,Biochemistry ,Wood ,General Biochemistry, Genetics and Molecular Biology ,Cell biology ,Transcriptome ,Populus ,Cell Wall ,Gene Expression Regulation, Plant ,Arabidopsis thaliana ,Secondary cell wall ,Woody plant ,Plant Proteins - Abstract
Wood is produced by accumulation of secondary xylem via proliferation and differentiation of the cambium cells in woody plants. Identifying the regulators involved in this process remains a challenging task. In this study, we isolated PagSAG101a, the homolog of Arabidopsis thaliana SAG101, from a hybrid poplar (Populus alba × P. glandulosa) clone 84K and investigated its role in secondary xylem development. PagSAG101a was expressed predominantly in lignified stems and localized in the nucleus. Compared with non-transgenic 84K plants, transgenic plants overexpressing PagSAG101a displayed increased plant height, internode number, stem diameter, xylem width, and secondary cell wall thickness, while opposite phenotypes were observed for PagSAG101a knock-out plants. Transcriptome analyses revealed that differentially expressed genes were enriched for those controlling cambium cell division activity and subsequent secondary cell wall deposition during xylem formation. In addition, the tandem CCCH zinc finger protein PagC3H17, which positively regulates secondary xylem width and secondary wall thickening in poplar, could bind to the promoter of PagSAG101a and mediate the regulation of xylem differentiation. Our results support that PagC3H17, downstream of PagC3H17, functions in wood development. This article is protected by copyright. All rights reserved.
- Published
- 2021
20. Brassinosteroid regulation of wood formation in poplar
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Li Zehua, Yu Liu, Shu‐Tang Zhao, Mengzhu Lu, Andrew Groover, Juan Du, Ying-Li Liu, and Suzanne Gerttula
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0106 biological sciences ,0301 basic medicine ,Cell type ,Physiology ,Secondary growth ,Cellular differentiation ,Plant Science ,Biology ,01 natural sciences ,03 medical and health sciences ,chemistry.chemical_compound ,Gene Expression Regulation, Plant ,Brassinosteroids ,Gene expression ,Brassinosteroid ,Cloning, Molecular ,Gene ,Plant Proteins ,Triazoles ,Herbaceous plant ,Plants, Genetically Modified ,Wood ,Cell biology ,Populus ,030104 developmental biology ,chemistry ,Secondary cell wall ,010606 plant biology & botany - Abstract
Brassinosteroids have been implicated in the differentiation of vascular cell types in herbaceous plants, but their roles during secondary growth and wood formation are not well defined. Here we pharmacologically and genetically manipulated brassinosteroid levels in poplar trees and assayed the effects on secondary growth and wood formation, and on gene expression within stems. Elevated brassinosteroid levels resulted in increases in secondary growth and tension wood formation, while inhibition of brassinosteroid synthesis resulted in decreased growth and secondary vascular differentiation. Analysis of gene expression showed that brassinosteroid action is positively associated with genes involved in cell differentiation and cell-wall biosynthesis. The results presented here show that brassinosteroids play a foundational role in the regulation of secondary growth and wood formation, in part through the regulation of cell differentiation and secondary cell wall biosynthesis.
- Published
- 2019
21. Development of a hyper-adhesive and attenuated Edwardsiella ictaluri strain as a novel immersion vaccine candidate in yellow catfish (Pelteobagrus fulvidraco)
- Author
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Ying Li Liu, Tian Tian He, Xiu Long Jiang, Shan Shan Sun, Long Kun Wang, Pin Nie, and Hai Xia Xie
- Subjects
Fish Diseases ,Infectious Diseases ,Bacterial Vaccines ,Immersion ,Enterobacteriaceae Infections ,Animals ,Edwardsiella ictaluri ,Vaccines, Attenuated ,Microbiology ,Bacterial Adhesion ,Catfishes - Abstract
Edwardsiella ictaluri, a Gram-negative intracellular pathogen, is the causative agent of enteric septicemia in channel catfish, and catfish aquaculture in China suffers heavy economic losses due to E. ictaluri infection. Vaccination is an effective control measure for this disease. In this study, an attenuated E. ictaluri strain was acquired through deletion mutation of the T3SS protein eseJ
- Published
- 2022
22. PagGRF12a interacts with PagGIF1b to regulate secondary xylem development through modulating PagXND1a expression in Populus alba × P. glandulosa
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Cheng Jiang, Jian-Jun Hu, Jinnan Wang, Houjun Zhou, Mengzhu Lu, Yanqiu Zhao, Ying-Li Liu, Shutang Zhao, Jihong Li, Fang Tang, and Xueqin Song
- Subjects
Chemistry ,Transgene ,fungi ,food and beverages ,Xylem ,Plant Development ,Plant Science ,Plants, Genetically Modified ,Biochemistry ,General Biochemistry, Genetics and Molecular Biology ,Cell biology ,Chromatin ,Plant development ,Populus ,Downregulation and upregulation ,Plant Growth Regulators ,Gene Expression Regulation, Plant ,Transcription factor ,Vascular tissue ,Woody plant ,Transcription Factors - Abstract
Growth-regulating factors (GRFs) are important regulators of plant development and growth, but their possible roles in xylem development in woody plants remain unclear. Here, we report that Populus alba × Papulus glandulosa PagGRF12a negatively regulates xylem development in poplar. PagGRF12a is expressed in vascular tissues. Compared to non-transgenic control plants, transgenic poplar plants overexpressing PagGRF12a exhibited reduced xylem width and plants with repressed expression of PagGRF12a exhibited increased xylem width. Xylem NAC domain 1 (XND1) encodes a NAC domain transcription factor that regulates xylem development and transcriptional analyses revealed that PagXND1a is highly upregulated in PagGRF12a-overexpressing plants and downregulated in PagGRF12a-suppressed plants, indicating that PagGRF12a may regulate xylem development through PagXND1a. Transient transcriptional assays and chromatin immunoprecipitation-polymerase chain reaction assays confirmed that PagGRF12a directly upregulates PagXND1a. In addition, PagGRF12a interacts with the GRF-Interacting Factor (GIF) PagGIF1b, and this interaction enhances the effects of PagGRF12a on PagXND1a. Our results indicate that PagGRF12a inhibits xylem development by upregulating the expression of PagXND1a.
- Published
- 2021
23. Identification and Characterization of EvpQ, a Novel T6SS Effector Encoded on a Mobile Genetic Element in Edwardsiella piscicida
- Author
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Duan You Li, Ying Li Liu, Xiao Jian Liao, Tian Tian He, Shan Shan Sun, Pin Nie, and Hai Xia Xie
- Subjects
Microbiology (medical) ,Genetics ,Sequence analysis ,Effector ,mobile genetic element ,Hypothetical protein ,lcsh:QR1-502 ,type VI secretion system ,EvpQ ,regulation ,Biology ,Microbiology ,lcsh:Microbiology ,effector ,Genomic island ,Gene cluster ,Edwardsiella piscicida ,Gene ,rpoS ,Original Research ,Type VI secretion system - Abstract
In this study, a hypothetical protein (ORF02740) secreted by Edwardsiella piscicida was identified. We renamed the ORF02740 protein as EvpQ, which is encoded by a mobile genetic element (MGE) in E. piscicida genome. The evpQ gene is spaced by 513 genes from type VI secretion system (T6SS) gene cluster. Low GC content, three tRNA, and three transposase genes nearby evpQ define this MGE that evpQ localizes as a genomic island. Sequence analysis reveals that EvpQ shares a conserved domain of C70 family cysteine protease and shares 23.91% identity with T3SS effector AvrRpt2 of phytopathogenic Erwinia amylovora. Instead, EvpQ of E. piscicida is proved to be secreted at a T6SS-dependent manner, and it can be translocated into host cells. EvpQ is thereof a novel T6SS effector. Significantly decreased competitive index of ΔevpQ strain in blue gourami fish (0.53 ± 0.27 in head kidney and 0.44 ± 0.19 in spleen) indicates that EvpQ contributes to the pathogenesis of E. piscicida. At 8-, 18-, and 24-h post-subculture into DMEM, the transcription of evpQ was found to be negatively regulated by Fur and positively regulated by EsrC, and the steady-state protein levels of EvpQ are negatively controlled by RpoS. Our study lays a foundation for further understanding the pathogenic role of T6SS in edwardsiellosis.
- Published
- 2021
24. Peritoneal dialysis effluent-derived exosomal miR-432-5p: an assessment tool for peritoneal dialysis efficacy
- Author
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Yan, Tong, Jun-Yan, Fang, A-Hui, Song, Hai, Deng, Pu, Li, Ze-Hui, Huang, Ou-Yang, Ji, Xiao-Lin, Ge, Tong-Ying, Zhu, and Ying-Li, Liu
- Subjects
enzymes and coenzymes (carbohydrates) ,Original Article ,General Medicine - Abstract
BACKGROUND: Ultrafiltration (UF) volume and peritoneal solute transport rate (PSTR) are common parameters used to evaluate the efficacy of peritoneal dialysis (PD) on individual patients. It is unclear whether the level of exosomal microRNA (miRNA) in peritoneal dialysis effluent (PDE) can predict UF or PSTR. This study was designed to investigate if there is a correlation between PDE exosomal miRNA (miR-432-5p) levels and various UF volumes and PSTRs in PD patients. It also aimed to explore the underlying mechanism of water and dialytic sodium removal (DSR). METHODS: The PSTR was quantified using the 4-hour (4 h) 3.86% dialysate to plasma creatinine ratio. The PDE exosomes (PDE-exo) were isolated by ultracentrifugation. An miRNA assay was used to identify the different miRNA in the PDE-exo of patients in a high (H; PSTR >0.65, n=5) and low (L; PSTR
- Published
- 2022
25. [Value evaluation and ecological compensation of endangered species from the perspective of stakeholders: A cases study with
- Author
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Yin-Bo, Zhang, Yang-Yang, Niu, Wen-Zhi, Wang, Hao, Qin, Ying-Li, Liu, and Jun-Sheng, Li
- Subjects
Elaeagnaceae ,Farmers ,Compensation and Redress ,Endangered Species ,Animals ,Humans ,Ecosystem - Abstract
The value evaluation of endangered species can provide important supports for policy-making in biodiversity conservation. We evaluated the stakeholders' preference and willingness to pay (WTP) from the perspective of stakeholders by the choice experiment method (CEM) and discussed the ecological compensation mechanism, with濒危物种价值评估可为生物多样性保护提供重要的政策支撑。本文以国家二级重点保护植物翅果油树为例,从利益相关者视角作为切入点,基于选择实验法评价其偏好和支付意愿(WTP),进而探讨生态补偿机制。基于管理部门、企业和农户三方利益相关者的利益诉求,分析确定了5个翅果油树的物种属性,即种植面积、产品分类、树苗品种、保护投入和农户收益。运用随机参数Logit模型分析了利益相关者对不同物种属性变量的偏好和支付意愿。结果表明: 受访者对“保护增加投入”偏好最强和支付意愿最高,每户为331.00 yuan·a
- Published
- 2020
26. Effect of roux-en Y gastric bypass surgery on patients with type 2 diabetes mellitus: A protocol of systematic review and meta-analysis
- Author
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De-zhi Ke, De-wang Su, Jincai Shang, Ying-Li Liu, Hao Tian, Ke-yan Chen, and Rong-rong Yao
- Subjects
Research design ,Adult ,medicine.medical_specialty ,type 2 diabetes mellitus ,MEDLINE ,Gastric Bypass ,CINAHL ,medicine.disease_cause ,roux-en Y gastric bypass surgery ,law.invention ,Diabetes Complications ,03 medical and health sciences ,0302 clinical medicine ,Postoperative Complications ,Randomized controlled trial ,Clinical Protocols ,Meta-Analysis as Topic ,law ,Study Protocol Systematic Review ,Medicine ,Humans ,030212 general & internal medicine ,Obesity ,Intensive care medicine ,business.industry ,Gastric bypass surgery ,effect ,Type 2 Diabetes Mellitus ,nutritional and metabolic diseases ,General Medicine ,Diabetes Mellitus, Type 2 ,Research Design ,030220 oncology & carcinogenesis ,Meta-analysis ,Inclusion and exclusion criteria ,business ,Systematic Reviews as Topic ,Research Article - Abstract
Background: Previous studies have reported that roux-en Y gastric bypass surgery (RYGBS) can benefit patients with type 2 diabetes mellitus (T2DM). However, their conclusions are still inconsistent. Thus, this study will aim to assess the effect of RYGBS for patients with T2DM. Methods: In this study, the electronic databases of MEDLINE, EMBASE, CENTRAL, CINAHL, AMED, and CNKI from inceptions to the present without any limitations to language and publication status. All randomized controlled trials on assessing the effect of RYGBS for patients with T2DM will be included in this study. Two independent authors will carry out study search and selection according to the previous designed inclusion and exclusion criteria. At the same time, 2 authors will independently evaluate the risk of bias assessment by Cochrane risk of bias tool. Any disagreements between 2 authors will be solved by a third author through discussion. RevMan 5.3 software will be utilized for statistical analysis. Results: This study will summarize the most recent studies and will provide a deeper understanding about using the effect of RYGBS for patients with T2DM. Conclusions: The findings of this study will present the existing evidence for the effect of RYGBS for patients with T2DM. Systematic review registration: INPLASY202040127.
- Published
- 2020
27. Potential mechanisms underlying the ameliorative effect of Lactobacillus paracasei FZU103 on the lipid metabolism in hyperlipidemic mice fed a high-fat diet
- Author
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Pingfan Rao, Li Ni, Xu-Cong Lv, Wei-Ling Guo, Zi-Rui Huang, Weidong Bai, Lianzhong Ai, Ying-Li Liu, Min Chen, and Xiao-Dan Yu
- Subjects
medicine.medical_specialty ,Lactobacillus paracasei ,030309 nutrition & dietetics ,medicine.drug_class ,Hyperlipidemias ,White adipose tissue ,Fatty acid degradation ,Diet, High-Fat ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,0404 agricultural biotechnology ,Internal medicine ,Hyperlipidemia ,medicine ,Animals ,0303 health sciences ,biology ,Bile acid ,food and beverages ,Lipid metabolism ,04 agricultural and veterinary sciences ,Lacticaseibacillus paracasei ,biology.organism_classification ,medicine.disease ,Lipid Metabolism ,040401 food science ,Gastrointestinal Microbiome ,Metabolic pathway ,Endocrinology ,chemistry ,Fatty acid elongation ,Food Science - Abstract
Lactobacillus paracasei FZU103, a probiotic previously isolated from the traditional brewing process of Hongqu rice wine, may have the beneficial effect of improving the disorder of lipid metabolism. This study aimed to determine the beneficial effects of L. paracasei FZU103 on improving hepatic lipid accumulation associated with hyperlipidemia. Results indicated that L. paracasei FZU103 intervention significantly inhibited the abnormal growth of body weight and epididymal white adipose tissue (eWAT), prevented the hypertrophy of epididymal adipocytes, ameliorated the biochemical parameters of serum and liver related to lipid metabolism in HFD-fed mice. Histological analysis also showed that the excessive accumulation oflipid dropletsin the livers induced by HFD-feeding was greatly alleviated by L. paracasei FZU103 intervention. In addition, L. paracasei FZU103 also promoted the excretion of bile acids (BAs) through feces. Metagenomic analysis revealed that oral supplementation with L. paracasei FZU103 significantly increased the relative abundance of Ruminococcus, Alistipes, Pseudoflavonifractor and Helicobacter, but decreased the levels of Blautia, Staphylococcos and Tannerella in HFD-fed mice. The relationships between lipid metabolic parameters and intestinal microbial phylotypes were also revealed by correlation heatmap and network. Furthermore, ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS)-based liver metabolomics demonstrated that L. paracasei FZU103 had a significant regulatory effect on the metabolic pathways of glycerophospholipid metabolism, fatty acid degradation, fatty acid elongation, unsaturated fatty acids biosynthesis, riboflavin metabolism, glycerolipid metabolism, primary bile acid biosynthesis, arachidonic acid metabolism, etc. Additionally, L. paracasei FZU103 intervention regulated expression of hepatic genes involved in lipid metabolism and bile acid homeostasis, and promoted fecal excretion of intestinal BAs. These findings present new evidence supporting that L. paracasei FZU103 has the potential to improve lipid metabolism, and could be used as a potential functional food for the prevention of hyperlipidemia.
- Published
- 2020
28. First case of Shewanella indica isolated from a Bryde's whale (Balaenoptera edeni) stranded in the northern Beibu Gulf, China
- Author
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Jun Ying Zhou, Jia Li, Richard William McLaughlin, Ying Li Liu, Xiao Ling Wan, Hia Xia Xie, Mo Chen, and Jinsong Zheng
- Subjects
0301 basic medicine ,DNA, Bacterial ,China ,Shewanella ,medicine.drug_class ,030106 microbiology ,Antibiotics ,Virulence ,Shewanella algae ,Microbial Sensitivity Tests ,Microbiology ,Agar plate ,03 medical and health sciences ,Hemolysin Proteins ,Bacterial Proteins ,biology.animal ,RNA, Ribosomal, 16S ,medicine ,Animals ,Molecular Biology ,Phylogeny ,biology ,Whale ,Biofilm ,General Medicine ,biology.organism_classification ,medicine.disease ,Hemolysis ,Bacterial Typing Techniques ,030104 developmental biology ,Biofilms ,Balaenoptera ,Bacteria - Abstract
The purpose of this study was to culture and characterise bacteria from an intact abscess on the skin of a dead Bryde's whale (Balaenoptera edeni) which stranded in the northern Beibu Gulf, China. To grow bacteria, samples from the abscess were added to blood agar. After incubation, yellowish mucous colonies were visualized. The bacterium was firstly recognised as Shewanella algae by the VITEK® 2 System. However, by using 16S rRNA gene sequencing the bacterium was finally identified as S. indica. To characterise the bacterium, antibiotic susceptibility and virulence factors, such as hemolysis and biofilm formation were investigated. The bacterium is capable of β-hemolysis and biofilm formation and it is also sensitive to several different classes of antibiotics, such as β-lactams, quinolones, and aminoglycosides. To date there have been no reports of this bacterium causing infections in humans or animals. However, in this study we described the first case of S. indica isolated from an intact abscess on the back of a Bryde's whale.
- Published
- 2020
29. Identification of the Genetic Requirements for Zinc Tolerance and Toxicity in
- Author
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Yun-Ying, Zhao, Chun-Lei, Cao, Ying-Li, Liu, Jing, Wang, Jie, Li, Shi-Yun, Li, and Yu, Deng
- Subjects
Saccharomyces cerevisiae Proteins ,Chlorides ,Zinc Compounds ,Mutant Screen Report ,zinc toxicity ,genomics ,Reactive oxygen species (ROS) ,Drug Tolerance ,Protein Interaction Maps ,Saccharomyces cerevisiae ,genetic screening ,Genome, Fungal ,Reactive Oxygen Species - Abstract
Zinc is essential for almost all living organisms, since it serves as a crucial cofactor for transcription factors and enzymes. However, it is toxic to cell growth when present in excess. The present work aims to investigate the toxicity mechanisms induced by zinc stress in yeast cells. To this end, 108 yeast single-gene deletion mutants were identified sensitive to 6 mM ZnCl2 through a genome-wide screen. These genes were predominantly related to the biological processes of vacuolar acidification and transport, polyphosphate metabolic process, cytosolic transport, the process utilizing autophagic mechanism. A result from the measurement of intracellular zinc content showed that 64 mutants accumulated higher intracellular zinc under zinc stress than the wild-type cells. We further measured the intracellular ROS (reactive oxygen species) levels of 108 zinc-sensitive mutants treated with 3 mM ZnCl2. We showed that the intracellular ROS levels in 51 mutants were increased by high zinc stress, suggesting their possible involvement in regulating ROS homeostasis in response to high zinc. The results also revealed that excess zinc could generate oxidative damage and then activate the expression of several antioxidant defenses genes. Taken together, the data obtained indicated that excess zinc toxicity might be mainly due to the high intracellular zinc levels and ROS levels induced by zinc stress in yeast cells. Our current findings would provide a basis to understand the molecular mechanisms of zinc toxicity in yeast cells.
- Published
- 2019
30. 938RHRK941 is responsible for Ubiquitin specific protease 48 nuclear translocation which can stabilize NF-κB (p65) in the nucleus
- Author
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Ying-Li Liu, Qing-Bao Tian, Jie Zheng, and Jing Yan
- Subjects
0301 basic medicine ,Protease ,Mechanism (biology) ,medicine.medical_treatment ,Regulator ,Chromosomal translocation ,General Medicine ,Biology ,Chromatin ,Cell biology ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,Genetics ,medicine ,Gene family ,Transcription factor ,Nucleus ,030217 neurology & neurosurgery - Abstract
The transcription factor NF-κB is a key regulator of cellular processes. A mechanism that contributes to timely termination of NF-κB activity is UPS-dependent degradation of p65 in the nucleus or on chromatin. The ubiquitin-specific protease that takes part in this process and its molecular mechanisms are shown in previous study, but which structural feature of USP48 was responsible for these effects is unknown. Here, we show that maybe the stability of NF-κB is controlled by proteasome-mediated degradation and ubiquitin-specific protease 48 (USP48), also known as synaptic ubiquitin-specific protease (synUSP) or USP31, can enhance NF-κB stability through proteasome-dependent regulation in the nucleus. USP48 contains a carboxyl-terminal nuclear localizing signal, 938RHRK941, which is responsible for its nuclear translocation. Our results demonstrate a more detailed mechanism for this member of the USP gene family in cellular processes.
- Published
- 2018
31. Obesity as a Risk Factor for Low Back Pain
- Author
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Dian-Wu Liu, Qing-Bao Tian, Ting-Ting Zhang, Zhen Liu, Ying-Li Liu, and Jing-Jing Zhao
- Subjects
medicine.medical_specialty ,Overweight ,Cohort Studies ,03 medical and health sciences ,0302 clinical medicine ,Risk Factors ,health services administration ,Odds Ratio ,medicine ,Humans ,Orthopedics and Sports Medicine ,Obesity ,030212 general & internal medicine ,Risk factor ,business.industry ,Publication bias ,Odds ratio ,Low back pain ,nervous system diseases ,Meta-analysis ,Cohort ,Physical therapy ,population characteristics ,Surgery ,Neurology (clinical) ,medicine.symptom ,business ,Low Back Pain ,Publication Bias ,030217 neurology & neurosurgery ,Cohort study - Abstract
A meta-analysis.To update the current knowledge about the association between overweight, obesity, and low back pain (LBP) risk, we conducted a meta-analysis of published cohort studies.The association between obesity and LBP risk has been the research focus in the past decade. However, available data from studies on the association between obesity and LBP remains debatable.An extensive English language literature retrieval regarding the association between overweight, obesity, and the risk of LBP incidence was conducted on PubMed and EMBASE databases through December 2015. Meta-analysis for all the included literature was performed by STATA 12.0 to summarize test performance with Forest plots after a heterogeneity test. Moreover, subgroup and sensitivity analyses were performed to examine the potential candidate-effect factors.A total of 10 cohort studies including 29,748 subjects satisfied the predefined eligibility criteria. The pooled odds ratio (OR) for overweight and obesity compared with normal weight was 1.15 [95% confidence interval (CI), 1.08-1.21) and 1.36 (95% CI, 1.18-1.57), respectively. Moreover, subgroup analysis proved that increased body mass index was associated with an increased incidence of LBP in both men (overweight: pooled OR=1.16, 95% CI, 1.04-1.31; obesity: pooled OR=1.36, 95% CI, 1.15-1.61) and women (overweight: pooled OR=1.24, 95% CI, 1.04-1.50; obesity: pooled OR=1.40, 95% CI, 1.08-1.82). There was no evidence of publication bias.Our findings consistently show that overweight and obesity are risk factors for LBP in men and women. Maintaining a healthy body weight may be one of the factors preventing the occurrence of LBP.Level 1.
- Published
- 2018
32. The impacts of nineteen mutations on the enzymatic activity of USP26
- Author
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Ying-Jun Mi, Ying-Li Liu, Jing Zhao, Jie Zheng, and Qing-Bao Tian
- Subjects
0301 basic medicine ,Recombinant Fusion Proteins ,Mutant ,Biology ,USP26 ,03 medical and health sciences ,0302 clinical medicine ,Plasmid ,Escherichia coli ,Genetics ,Site-directed mutagenesis ,Gene ,chemistry.chemical_classification ,030219 obstetrics & reproductive medicine ,Wild type ,General Medicine ,Molecular biology ,Enzyme assay ,Cysteine Endopeptidases ,030104 developmental biology ,Enzyme ,chemistry ,Biochemistry ,Transcription Termination, Genetic ,Mutation ,Mutagenesis, Site-Directed ,biology.protein ,Mutant Proteins ,Plasmids - Abstract
Objective The association between mutations in the USP26 gene and male infertility has been studied intensively. However, the biological function of the mutant proteins remains to be elucidated. To confirm the effects of the reported mutations, we analyse the enzyme activity of USP26 between the wild-type and the variants from a molecular perspective. Methods Using pGEX-USP26 as a template, site-directed mutagenesis was conducted to generate nineteen USP26 mutant plasmids. Using Ub-Met-β-gal and GST-Ub52 as model substrates, a USP cleavage assay was conducted to assess the enzymatic activities of the mutants. Results The enzyme activity of the Q156H mutant disappeared, but the other 18 mutants had the same activity as the wild type. E174# and E189# were terminal mutants, but they still had the same activity as the wild type. When we constructed the transcription terminal mutants E174#(1-522 bp), E174#(523-2742 bp), E189#(1-567 bp) and E189#(568-2742 bp) artificially, the enzyme activity of these four mutants disappeared. Conclusions We have successfully constructed nineteen mutants of USP26. The enzyme activity of the Q156H mutant disappeared, but the enzyme activities of the other 18 mutants were the same as that of the wild type.
- Published
- 2018
33. Effects of telmisartan on improving leptin resistance and inhibiting hepatic fibrosis in rats with non-alcoholic fatty liver disease
- Author
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Qiu‑Zan Zhang, Li‑Na Fu, Xiu‑Ru Wang, Ying‑Li Liu, Jing Zhang, Bang‑Mao Wang, and Yan‑Rong Wang
- Subjects
0301 basic medicine ,Cancer Research ,medicine.medical_specialty ,Oncogene ,Leptin ,medicine.medical_treatment ,Fatty liver ,Articles ,General Medicine ,Biology ,medicine.disease ,Molecular medicine ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Endocrinology ,Immunology and Microbiology (miscellaneous) ,Apoptosis ,Internal medicine ,medicine ,030211 gastroenterology & hepatology ,Telmisartan ,Hepatic fibrosis ,Saline ,medicine.drug - Abstract
The present study aimed to investigate the impacts of telmisartan (TEL) on hepatic fibrosis, serum leptin, leptin protein in liver tissue and its mRNA expression level in rats with non-alcoholic fatty liver disease (NAFLD). Male Sprague Dawley rats were randomly divided into the control (N), model (M), polyene phosphatidylcholine (P) and TEL (T) groups. Group M and the intervention groups were given a high-fat diet for 12 weeks to induce NAFLD, followed by 4 weeks of intragastric administration of normal saline (1.0 ml/kg/day), polyene phosphatidylcholine (PPC; 123.1 mg/kg/day) and TEL (8 mg/kg/day). The liver tissue was then assessed for the NAFLD activity score and fibrosis score (FS), and serum biochemistry and leptin levels were determined. Additionally, leptin protein expression levels were examined by western blotting and the expression of leptin mRNA was investigated by reverse transcription-polymerase chain reaction. TEL significantly improved FS in rats (P0.05). TEL reduced serum leptin, leptin protein and its mRNA in the liver tissue of NAFLD rats, and improved the pathological indicators of liver fibrosis.
- Published
- 2017
34. Identification of candidate substrates of ubiquitin-specific protease 13 using 2D-DIGE
- Author
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Dian-Wu Liu, Li-Juan Tang, Ying-Jun Mi, Qing-Bao Tian, Ying-Li Liu, Jian-Min Wang, and Su-Fen Qi
- Subjects
0301 basic medicine ,Polyadenylation ,liquid chromatography-mass spectroscopy/mass spectrometry ,enzymatic activity ,Mutation, Missense ,Cell Line ,Substrate Specificity ,Phosphoglycerate mutase ,03 medical and health sciences ,Ubiquitin ,Annexin ,Endopeptidases ,Genetics ,Humans ,two-dimensional difference gel electrophoresis ,Electrophoresis, Gel, Two-Dimensional ,Methylosome ,Thimet oligopeptidase ,biology ,vinculin ,Adenylosuccinate synthase ,General Medicine ,Articles ,Vinculin ,Molecular biology ,030104 developmental biology ,Biochemistry ,Amino Acid Substitution ,biology.protein ,Ubiquitin-Specific Proteases ,ubiquitin-specific protease 13 - Abstract
The present study aimed to identify candidate substrates of ubiquitin-specific protease (USP)13 using two-dimensional fluorescence difference gel electrophoresis (2D-DIGE). USP13 is a well-characterized member of the USP family, which regulates diverse cellular functions by cleaving ubiquitin from ubiquitinated protein substrates. However, existing studies indicate that USP13 has no detectable hydrolytic activity in vitro. This finding implies that USP13 likely has different substrate specificity. In this study, a USP cleavage assay was performed using two different types of model substrates (glutathione S-transferase-Ub52 and ubiquitin-β-galactosidase) to detect the deubiquitinating enzyme (DUB) activity of USP13. In addition, a proteomic approach was taken by using 2D-DIGE to detect cellular proteins whose expressoin is significantly altered in 293T cell lines following the overexpression of USP13 or its C345S mutant (the catalytically inactive form). The data indicated that USP13 still has no detectable DUB activity in vitro nor does C345S. The results of 2D-DIGE demonstrated that the expression of several proteins increased or decreased significantly in 293T cells following the overexpression of USP13. Mass spec-troscopy analysis of gel spots identified 7 proteins, including 4 proteins with an increased expression, namely vinculin, thimet oligopeptidase, cleavage and polyadenylation specific factor 3, and methylosome protein 50, and 3 proteins with a decreased expression, namely adenylosuccinate synthetase, annexin and phosphoglycerate mutase. In addition, in the samples of 293T cell lines after the overexpression of USP13 and USP13 C345S, vinculin exhibited an increased expression, suggesting that it may be a candidate substrate of USP13. However, sufficient follow-up validation studies are required in order to determine whether vinculin protein directly interacts with USP13.
- Published
- 2017
35. Edwardsiella piscicida type III protein EseJ suppresses apoptosis through down regulating type 1 fimbriae, which stimulate the cleavage of caspase-8
- Author
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Tian Tian He, Pin Nie, Ying Zhou, Ying Li Liu, Ai Hua Li, Duan You Li, and Hai Xia Xie
- Subjects
Lipopolysaccharides ,Immunology ,Fimbria ,Apoptosis ,Biology ,Caspase 8 ,Microbiology ,Type three secretion system ,Cell Line ,03 medical and health sciences ,Epitopes ,Fish Diseases ,Mice ,Bacterial Proteins ,Virology ,Type III Secretion Systems ,Animals ,Secretion ,Zebrafish ,030304 developmental biology ,0303 health sciences ,TUNEL assay ,030306 microbiology ,Effector ,Caspase 3 ,Macrophages ,Enterobacteriaceae Infections ,Molecular biology ,Caspase 9 ,Terminal deoxynucleotidyl transferase ,Edwardsiella ,Fimbriae, Bacterial ,Larva ,Host-Pathogen Interactions - Abstract
The type III secretion system effector EseJ plays a regulatory role inside bacteria. It suppresses the adherence of Edwardsiella piscicida (E. piscicida) to host epithelial cells by down regulating type 1 fimbriae. In this study, we observed that more macrophages infected with ΔeseJ strain of E. piscicida detached as compared with those infected with the wild-type (WT) strain. Terminal deoxynucleotidyl transferase dUTP nick-end labelling (TUNEL) staining and cleaved caspase-3 examination revealed that the detachment is due to increased apoptosis, suggesting that EseJ suppresses macrophage apoptosis. However, apoptosis inhibition by EseJ is not relative to a type III secretion system (T3SS) and is not related to EseJ's translocation. Since EseJ negatively regulates type 1 fimbriae, murine J774A.1 cells were infected with ΔeseJΔfimA or ΔeseJΔfimH strains. It was demonstrated that ΔeseJ stimulates macrophage apoptosis through type 1 fimbriae. Moreover, we found that infecting J774A.1 cells with the ΔeseJ strain increased levels of cleaved caspase-8, caspase-9, and caspase-3, demonstrating that EseJ inhibits apoptosis through either an extrinsic or a combination of extrinsic and intrinsic pathways. Pre-treatment of macrophages with caspase-8 inhibitor prior to infection with the ΔeseJ strain decreased the levels of cleaved caspase-8, caspase-9, and caspase-3, indicating that the ΔeseJ strain stimulates apoptosis, mainly through an extrinsic pathway by up regulating type 1 fimbriae. Zebrafish larvae or blue gourami fish infected with the ΔeseJ strain consistently exhibited higher apoptosis than those infected with the E. piscicida WT strain or ΔeseJΔfimA strain. Taken together, we revealed that the T3SS protein EseJ of E. piscicida inhibits host apoptosis, mainly through an extrinsic pathway by down regulating type 1 fimbriae.
- Published
- 2019
36. The Edwardsiella piscicida Type III Translocon Protein EseC Inhibits Biofilm Formation by Sequestering EseE
- Author
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Pin Nie, Jia Yi, Tian Tian He, Hongbing Yu, Lu Yi Liu, Hai Xia Xie, and Ying Li Liu
- Subjects
Receptors, Peptide ,Virulence Factors ,Operon ,Receptors, Cytoplasmic and Nuclear ,Virulence ,Genetics and Molecular Biology ,Applied Microbiology and Biotechnology ,Microbiology ,Type three secretion system ,Fish Diseases ,03 medical and health sciences ,Bacterial Proteins ,Type III Secretion Systems ,Animals ,030304 developmental biology ,0303 health sciences ,Membrane Glycoproteins ,Ecology ,biology ,030306 microbiology ,Chemistry ,Effector ,Calcium-Binding Proteins ,Edwardsiella tarda ,Biofilm ,Gene Expression Regulation, Bacterial ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,Translocon ,Edwardsiella ,Biofilms ,Gene Deletion ,Intracellular ,Food Science ,Biotechnology - Abstract
The type III secretion system (T3SS) is one of the most important virulence factors of the fish pathogen Edwardsiella piscicida. It contains three translocon proteins, EseB, EseC, and EseD, required for translocation of effector proteins into host cells. We have previously shown that EseB forms filamentous appendages on the surface of E. piscicida, and these filamentous structures mediate bacterial cell-cell interactions promoting autoaggregation and biofilm formation. In the present study, we show that EseC, but not EseD, inhibits the autoaggregation and biofilm formation of E. piscicida. At 18 h postsubculture, a Delta eseC strain developed strong autoaggregation and mature biofilm formation, accompanied by enhanced formation of EseB filamentous appendages. This is in contrast to the weak autoaggregation and immature biofilm formation seen in the E. piscicida wild-type strain. EseE, a protein that directly binds to EseC and also positively regulates the transcription of the escC-eseE operon, was liberated and showed increased levels in the absence of EseC. This led to augmented transcription of the escC-eseE operon, thereby increasing the steady-state protein levels of intracellular EseB, EseD, and EseE, as well as biofilm formation. Notably, the levels of intracellular EseB and EseD produced by the Delta eseE and Delta eseC Delta eseE strains were similar but remarkably lower than those produced by the wild-type strain at 18 h postsubculture. Taken together, we have shown that the translocon protein EseC inhibits biofilm formation through sequestering EseE, a positive regulator of the escC-eseE operon. IMPORTANCE Edwardsiella piscicida, previously known as Edwardsiella tarda, is a Gram-negative intracellular pathogen that mainly infects fish. The type III secretion system (T3SS) plays a pivotal role in its pathogenesis. The T3SS translocon protein EseB is required for the assembly of filamentous appendages on the surface of E. piscicida. The interactions between the appendages facilitate autoaggregation and biofilm formation. In this study, we explored the role of the other two translocon proteins, EseC and EseD, in biofilm formation. We have demonstrated that EseC, but not EseD, inhibits the autoaggregation and biofilm formation of E. piscicida, providing new insights into the regulatory mechanism involved in E. piscicida biofilm formation.
- Published
- 2019
37. Bridging the 'gap' in systematic conservation planning
- Author
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Jing-xuan Fu, Ying-li Liu, Feng Zhang, Yin-bo Zhang, Nathan Phillips, and Ming-gang Zhang
- Subjects
0106 biological sciences ,Nature reserve ,Conservation planning ,Prioritization ,Ecology ,business.industry ,010604 marine biology & hydrobiology ,Environmental resource management ,Biodiversity ,010603 evolutionary biology ,01 natural sciences ,Biodiversity hotspot ,Network planning and design ,Biodiversity conservation ,Geography ,Threatened species ,business ,Nature and Landscape Conservation - Abstract
Biodiversity hotspots at the global and national scale have contributed important information for biodiversity conservation; however, in hotspot designation two kinds of “gap” greatly limit conservation planning, which this study identifies as the “scale gap” and the “conservation gap”. To address the gap, we integrated systematic conservation planning (SCP) with gap analysis to optimize solutions of protected areas planning at local scales. In this study, we presented a quantitative spatial methodology for prioritization and downscaled to a planning case study in the province of Shanxi, China. First, the spatial distribution of 54 threatened plant species were mapped at fine resolution, and scenarios of conservation targets for taxa were generated according to species attributes, which are both the necessary inputs in selection algorithm of priority conservation. Then we determined 17 priority conservation areas using SCP, which only cover ∼5% of the total area but could represent 100% of the threatened plant species in Shanxi Province. We confirmed that priority conservation areas determined based on SCP can achieve maximum efficiency of conservation, especially considering representation of small-ranged species. Further, through overlapping priority conservation areas with nature reserves, six conservation gaps were identified for future conservation efforts. Our findings provide suggestions for protected areas network planning of botanical conservation in real-world contexts. The proposed method of integrating SCP with gap analysis can be generally used in bridging the gap between biodiversity priority areas and protected areas in proactive planning and management protocol for biodiversity conservation.
- Published
- 2016
38. Therapy of auricular subcutaneous penetration needling combined with row acupuncture at the meridian sinew in 103 cases of prolapse of lumbar intervertebral disc
- Author
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Xian-bing Hou, Ying-li Liu, Li-chun Wang, Chun-Sheng Jia, Wei-wei Lu, and Shu-chang Song
- Subjects
medicine.medical_specialty ,Dry needling ,Combined treatment ,Meridian (perimetry, visual field) ,genetic structures ,Complementary and alternative medicine ,business.industry ,medicine ,Acupuncture ,Clinical efficacy ,business ,Lumbar intervertebral disc ,Surgery - Abstract
Objective To observe the clinical efficacy of auricular subcutaneous penetration needling combining with row acupuncture at meridian sinew on prolapse of lumbar intervertebral disc. Methods One hundred and three patients with prolapse of lumbar intervertebral disc were treated with a combined treatment of auricular subcutaneous penetration needling and row acupuncture at meridian sinew. Results Of 103 patients, 46 cases were cured, 33 were markedly effective, 21 effective and 3 ineffective. The total effective rate was 97.1% (100/103). Conclusion The combined treatment of auricular subcutaneous penetration needling and row acupuncture at meridian sinew has a significant efficacy for the treatment of prolapse of lumbar intervertebral disc.
- Published
- 2015
39. Alpha-synuclein (SNCA) polymorphisms and susceptibility to Parkinson's disease: A meta-analysis
- Author
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Wei Han, Jing Zhao, Ying-Jun Mi, Qing-Bao Tian, Dian-Wu Liu, and Ying-Li Liu
- Subjects
Alpha-synuclein ,Parkinson's disease ,business.industry ,Parkinson Disease ,Single-nucleotide polymorphism ,Odds ratio ,Disease ,Ethnic populations ,Bioinformatics ,medicine.disease ,Polymorphism, Single Nucleotide ,Cellular and Molecular Neuroscience ,Psychiatry and Mental health ,chemistry.chemical_compound ,chemistry ,Risk Factors ,Meta-analysis ,alpha-Synuclein ,medicine ,Humans ,SNP ,Genetic Predisposition to Disease ,business ,Publication Bias ,Genetics (clinical) - Abstract
It has been reported that single nucleotide polymorphisms (SNPs) of Alpha-synuclein (SNCA) are associated with Parkinson's disease (PD). Some researchers have attempted to validate this finding in various ethnic populations. The results of studies concerning SNCA polymorphisms and PD susceptibility remain conflicting. To evaluate the association between these SNPs and PD, the authors conducted a series of meta-analyses using a predefined protocol. Databases including PubMed, MEDLINE and PD gene were searched to identify relevant studies. Odds ratios (ORs) with 95% confidence intervals (CIs) were used to assess the strength of the association. All analyses were calculated using STATA11.0. A total of 19 studies on the SNPS rs181489, rs356186, rs356219, rs894278, rs2583988, rs2619363, rs2619364, rs2737029, rs10005233 and rs11931074 were included. This meta-analysis showed that eight out of these 10 candidate SNPs may be associated with PD risk. Significant association was found between PD and the following SNPs: rs181489, rs356186, rs356219, rs894278 rs2583988, rs2619364, rs10005233 and rs11931074. Among these SNPs, rs356186 was found to be the only SNP that may play a protective role in Parkinson's disease. These results suggest that the SNCA gene may be associated with PD.
- Published
- 2015
40. Identification, expression analysis, and antibacterial activity of NK-lysin from common carp Cyprinus carpio
- Author
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Hai Xia Xie, Pan Ting Liu, Ming Cheng Wang, Qian Zhang, Pin Nie, Chuan Feng Li, En Zhong Li, Gai Ling Wang, and Ying Li Liu
- Subjects
0301 basic medicine ,Fish Proteins ,Carps ,Proteolipids ,Lysin ,Aquatic Science ,medicine.disease_cause ,complex mixtures ,Microbiology ,03 medical and health sciences ,Common carp ,Fish Diseases ,Immune system ,medicine ,Environmental Chemistry ,Animals ,Amino Acid Sequence ,Gene ,Escherichia coli ,Phylogeny ,biology ,Base Sequence ,Gene Expression Profiling ,Edwardsiella tarda ,04 agricultural and veterinary sciences ,General Medicine ,Antimicrobial ,biology.organism_classification ,Immunity, Innate ,Aeromonas hydrophila ,030104 developmental biology ,Gene Expression Regulation ,040102 fisheries ,bacteria ,0401 agriculture, forestry, and fisheries ,Gram-Negative Bacterial Infections ,Sequence Alignment - Abstract
Natural killer lysin (NK-lysin), produced by cytotoxic T lymphocytes and natural killer cells, is a cationic antimicrobial peptide that has a broad antimicrobial spectrum, including bacteria, viruses, and parasites. Nevertheless, the implication of NK-lysin in the protection against bacterial infection is not aware in common carp. In this study, six different NK-lysin genes (nkl1, nkl2, nkl3, nkl4, nkl5 and nkl6) were identified in the common carp genome. Each of the mature peptides of common carp NK-lysin has six well-conserved cysteine residues, and shares a Saposin B domain, characteristic of saposin-like protein (SALIP) family. The gene nkl1 contains 5 extrons and 4 introns, and nkl2, nkl3, nkl4 or nkl5 contains 4 extrons and 3 introns, however, the nkl6 has 3 extrons and 2 introns. By quantitative real-time PCR, nkl2 transcripts were predominantly expressed in spleen of healthy common carp, while elevated mainly in gill and spleen upon Aeromonas hydrophila infection. The recombinant NK-lysin-2 purified from Pichia pastoris shows antibacterial activity against Staphylococcus aureus (Gram-positive), and Escherichia coli M15, Aeromonas hydrophila, as well as Edwardsiella tarda (Gram-negative), the latter two are important pathogens of aquaculture. Our results indicate that NK-lysin in common carp might play an important role in fish immune response by enhancing antibacterial defense against bacterial pathogens.
- Published
- 2017
41. Control of 2D Minimally Persistent Formations with the Fault Tolerance of Three Co-Leaders in Cycle
- Author
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Zhen Su, Xiao Kun Ying, Rui Ying Zhao, Yi Jian Zhang, Bo Qing Xu, Ying Li Liu, Liu Yang, Tao Ma, Hong Li, Cao Hu, Ying Xing, and Qiang Qu
- Subjects
Engineering ,Basis (linear algebra) ,business.industry ,Control theory ,Plane (geometry) ,Principal (computer security) ,Control (management) ,General Engineering ,Stability (learning theory) ,Fault tolerance ,Kinematics ,business ,First order - Abstract
This paper mainly addresses a novel control law with rigidity matrix based on three co-leaders minimally persistent formations in the plane. This control law particularly considers the fault tolerance of the leaders, and in this way, the three co-leaders model is better than leader-first follower model, leader-remote follower model, etc. in persistent formation. Firstly, the first order kinematic model is adopted for every agent. Then the fundamental moving principal of the leaders and the followers are described in detail. On the basis of these principals, the control law with the rigidity matrix for the whole formation is proposed. Moreover, the stability analysis is also supplied. Finally, simulations show that the proposed controllers ensure the group formation stabilized to maintain the rigid shape, while the distances between the agents remain unchanged.
- Published
- 2014
42. Experimental Studies on Basic Performance and Application of Self-Compacting Concrete Using Waste Brick Recycled Coarse Aggregate
- Author
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Ying Li Liu, Han Qing Liu, and Lin Gao
- Subjects
Sustainable development ,Brick ,Materials science ,Aggregate (composite) ,Water–cement ratio ,Waste management ,General Engineering ,Construction waste ,Environmental pollution ,Gradation ,Land resources - Abstract
With rapid economic development in China, the frequency of that new buildings replace old buildings increases gradually in rural areas, which inevitably results in the emergence of a large amount of waste clay brick. The processing of construction waste takes up a lot of land resources and worsen the existing rural environmental pollution situation. using waste clay brick to prepare recycled coarse aggregate concrete not only can effectively eliminate construction waste and reduce the consumption of original coarse aggregate and thus the cost of buildings, but also solves a series of environmental problems—it conforms to the concept of "green building" and stands in line with the strategic objectives of sustainable development. This paper researched on the influence of recycled coarse aggregate gradation and water cement ratio to the strength of recycled concrete at first, then experimentally verified the application feasibility of recycled coarse aggregate self-compacting concrete.
- Published
- 2014
43. Experimental Study on Tension Performance of Anchor Group
- Author
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Fu Quan Xu, Jun Hong Huan, Tie Zhu Jiang, and Ying Li Liu
- Subjects
Engineering ,Ultimate load ,Tension (physics) ,business.industry ,Group (periodic table) ,General Engineering ,Geotechnical engineering ,Undercut ,Structural engineering ,Conical surface ,business ,Failure mode and effects analysis - Abstract
The torque-controlled expansion anchors, undercut anchors,bonded anchors and special inverted conical chemical anchors subjected to tension load, which including single anchor, two anchors, four anchors and eight anchors are tested. Failure mode and tension performance of anchor group are investigated, and test value and calculative value of ultimate load are compared. Experimental results show that some anchor group occur the concrete cone failure under tension load, and others occur steel failure under tension load. Test value and calculative value of ultimate load are compared, and the result meets well.
- Published
- 2014
44. Influence of SiO2 Additive on the High-Frequency Magnetic Properties of Sr-Doped Co2Z Hexaferrite
- Author
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Ying Li Liu, Yu Wang, Li Yun Gan, Huai Wu Zhang, and Kai Yang
- Subjects
Materials science ,Mechanical Engineering ,Doping ,Analytical chemistry ,Mineralogy ,Condensed Matter Physics ,Microstructure ,Grain size ,Mechanics of Materials ,Phase composition ,Initial permeability ,Permeability measurements ,Ferrite (magnet) ,General Materials Science - Abstract
In this work, SiO2has been selected to be introduced into the system to improve the high-frequency magnetic properties of Ba1.5Sr1.5Co2Fe24O41hexaferrites. The influence of SiO2additive on the phase composition, microstructures and high-frequency magnetic properties of the samples prepared by solid-state reaction were investigated. The results indicate that the major phase of doped samples is Z-type ferrite. The addition of SiO2causes a decrease of the average grain size. Permeability measurements indicate that SiO2doping significantly improves the magnetic characteristics of those samples. With the doping content of SiO2increasing from 0 to 8wt%, the initial permeability decreases from 5.8 to 2.6, and the cut-off frequency rises up from 1.5GHz to over 1.8GHz. Meanwhile, the quality factor of the samples sintered at 1200°C varies with the SiO2content, and a maximum quality factor at 1GHz of Q=14 was found for x=4wt%. The improvements of magnetic properties and the high quality factors are crucial for applications of Z-type hexaferrites in high-frequency communication systems.
- Published
- 2014
45. Study of the Anchorage Seismic Performance for Beam-Ends Strengthened by Bonded Rebars
- Author
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Li Xing Tao, Jun Hong Huan, Fu Quan Xu, and Ying Li Liu
- Subjects
Reciprocating motion ,Engineering ,Yield (engineering) ,business.industry ,General Engineering ,Structural engineering ,Composite material ,Reinforcement ,business ,Beam (structure) - Abstract
This article firstly designed four specimens, including a contrastive specimen and three chemical anchorage reinforcement specimens which reinforcing the beam ends in different ways and then applied low-cycle reciprocating to the four specimens. This paper analyzed the anchor failure performance of the anchorage specimens by testing and data .The research concluded that the chemical anchorage can meet the component single drawing to components yield without failure. With the increasing of the loading series and the cycles improving, the parts of the anchor bars were pulled out.
- Published
- 2014
46. Magnetic Properties and Microwave Loss of Polycrystalline M-Type Barium Hexaferrite Thick Films by Tape Casting
- Author
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Daming Chen, Jie Li, Yuan Xun Li, Huai Wu Zhang, and Ying Li Liu
- Subjects
Tape casting ,Materials science ,business.industry ,Mechanical Engineering ,Condensed Matter Physics ,Magnetocrystalline anisotropy ,Magnetic hysteresis ,Ferromagnetic resonance ,Magnetization ,Nuclear magnetic resonance ,Mechanics of Materials ,Remanence ,Optoelectronics ,General Materials Science ,Texture (crystalline) ,business ,Microwave - Abstract
M-type barium hexaferrite (BaM) is a promising gyromagnetic material for self-biased microwave\millimeter wave devices because of its large uniaxial magnetocrystalline anisotropy and low microwave loss in high frequency. Due to the limitation of growth conditions, it is difficult to deposit BaM films with enough thickness by PLD, MBE and Magnetron Sputtering for practical application. However, it is demonstrated in present experiment that large area polycrystalline BaM thick films (500μm) with self-biasing (high remanence) and low microwave loss can be successfully fabricated by tape casting. X-ray diffraction and Scanning electron microscopy results indicate that these BaM thick films have highly c-axis oriented crystallographic texture with hexagonal morphology. Magnetic hysteresis loops reveal that samples exhibit excellent properties with a saturate magnetization (4πMs) of 3606G, a high squareness ratio (Mr/Ms) of 0.82. In addition, ferromagnetic resonance (FMR) measurement shows that the FMR linewidth is as small as 431Oe at 48GHz. These parameters ensure these BaM thick films are potentially useful for self-biased microwave\millimeter wave devices such as circulator, phase shifter and filter.
- Published
- 2014
47. Experimental Studies on the Influence of Gradation of Recycled Coarse Aggregates to the Strength of Self-Compacting Concrete
- Author
-
Han Qing Liu, Ying Li Liu, and Lin Gao
- Subjects
Materials science ,Aggregate (composite) ,Geotechnical engineering ,Gradation ,General Medicine - Abstract
To make full use of waste clay bricks, we made recycled coarse aggregate by waste clay bricks and used it in self-compacting recycled concrete. In order to improve the utilization rate of recycled coarse aggregate and realize good workability, we adopted skeleton dense accumulation method. With the fitted curve method, we obtained an accumulation curve of recycled coarse aggregate with reasonable gradation. Under the same conditions, we made contrast test with the recycled coarse aggregate by different gradation way. Through contrast test, we found that the recycled concrete with skeleton dense accumulation method has better performance in strength and workability, and his method improved the utilization rate of the recycled coarse aggregate.
- Published
- 2014
48. The Application of CFRP Reinforced Concrete Beams
- Author
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Jin Long Zhao, Ying Li Liu, and Bing Xuan Du
- Subjects
Materials science ,Carbon fiber composite ,Life span ,business.industry ,Frame (networking) ,General Medicine ,Bearing capacity ,Structural engineering ,business ,Reinforced concrete ,Ductility ,Reinforcement ,Beam (structure) - Abstract
This paper expounds the excellent characteristics of CFRP in engineering application, analyzes the scope of application of CFRP reinforced plastics.This paper introduces the principle and the carbon fiber composite reinforcement method and frame beams and carbon fiber reinforced beam in the structure of the calculation results were analyzed .By SAP2000 software analyzing, results show that after using CFRP reinforced plastics has dramatically enhanced the bearing capacity of the frame beam, improve the ductility of the components, prolong the life span of the structure .
- Published
- 2013
49. PUSHOVER Analysis in the Application of the Framework Structure Reinforcement
- Author
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Ying Li Liu, Jin Long Zhao, and Bing Xuan Du
- Subjects
Structure (mathematical logic) ,Engineering ,Data model ,business.industry ,Property (programming) ,General Medicine ,Structural engineering ,business ,Reinforcement ,Incremental Dynamic Analysis - Abstract
This paper firstly introduces the basic principle of PUSHOVER analysis and general steps of analysis. And then use the actual construction example to illustrate its application. This paper aims to evaluate the anti-seismic property through setting the parameters, building the data model and establishing the PUSHOVER conditions, and then do the static elastoplastic analysis. When the anti-seismic property of the building can not meet the necessary standard, the strengthening is needed. What is more, this paper employs the steel plate for reinforcement since its advantages.
- Published
- 2013
50. Effects of off-stoichiometry and density on the magnetic and magneto-optical properties of yttrium iron garnet films by magnetron sputtering method.
- Author
-
Qing-Hui Yang, Huai-Wu Zhang, Qi-Ye Wen, and Ying-Li Liu
- Subjects
STOICHIOMETRY ,YTTRIUM iron garnet ,THERMOMAGNETISM ,FERRITES ,SILICATE minerals ,MAGNETRON sputtering - Abstract
Yttrium iron garnet films were deposited on Si and Si/CeO
2 substrates by magnetron sputtering method followed by postannealing. By varying the fabrication parameters such as sputtering atmosphere, sputtering power, and annealing atmosphere, single phase garnet films were obtained with different off-stoichiometry and film density. The dependence of cation ratio, magnetic and magneto-optical characteristics, and absorption coefficient were systemically investigated. The results reveal that a proper oxygen pressure in both sputtering and annealing process give rise to a small cation ratio of (Fe2+ or Fe4+ )/Fe3+ , thus is beneficial to obtain large saturation magnetization, large Faraday rotation, and small optical absorption. The sputtering power can also affect the properties of the film through changing the film density. Our results indicate that the properties of sputtering deposited yttrium iron garnet (YIG) film can be easily tuned and optimized by modifying the off-stoichiometry and density of the film, thus provides flexibility to fabricate YIG film for extensive applications. [ABSTRACT FROM AUTHOR]- Published
- 2010
- Full Text
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