46 results on '"Yinghu Chen"'
Search Results
2. Factors associated with afebrile presentation and delayed defervescence of bacterial meningitis in children under 3 years of age: a multi-centre retrospective analysis
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Lin He, Haijing Li, Zhigang Zhang, Hejia Ge, Hongwei Wang, Mengquan Zhu, Zhiwei Xu, Jiening Zhang, Sheng Fang, Chuanze Hu, Lijun Qian, Huifang Xu, Yinna Yao, Shengfu Yuan, Jiajun Zhu, Chaosheng Lu, Jishan Zheng, Junsheng Li, Qi Jiang, Huiqing Xu, Lihua Chen, Shiqiang Shang, and Yinghu Chen
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Fever ,Defervescence time ,Bacterial meningitis ,Children ,Pediatrics ,RJ1-570 - Abstract
Abstract Background This multi-center study aimed to identify factors affecting fever and delayed defervescence in bacterial meningitis (BM) patients under 3 years of age because of the variability of fever in this patient population. Methods Only BM patients under 3 years treated at 49 centers in China from November 2018 to end-April 2021 were included in the study. Univariate and multivariate logistic regression analyses were performed to determine factors associated with afebrile presentation and fever of delayed defervescence. Results A total of 863 BM patients under 3 years were included in the study. Coagulase negative staphylococcus was associated with afebrile presentation (OR = 1.176), while septicaemia and ear-nose-throat infections were associated with fever (P
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- 2023
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3. Clinical characteristics and antibiotic resistance profile of invasive MRSA infections in newborn inpatients: a retrospective multicenter study from China
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Xia Wu, Chuanqing Wang, Leiyan He, Hongmei Xu, Chunmei Jing, Yinghu Chen, Jikui Deng, Aiwei Lin, Huiling Deng, Huijun Cai, Yiping Chen, Jinhong Yang, Ting Zhang, Qing Cao, Jianhua Hao, Yuanyuan Huang, and Hui Yu
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Methicillin-resistant Staphylococcus aureus ,Invasive infection ,Clinical characteristics ,Antimicrobial resistance ,Neonates ,Pediatrics ,RJ1-570 - Abstract
Abstract Background Methicillin-resistant Staphylococcus aureus (MRSA) can cause invasive infections with significant mortality in neonates. This study aimed to analyze the clinical characteristics and antibiotic resistance profiles of invasive MRSA infections and determine risk factors associated with invasive MRSA infections in newborn inpatients. Methods This multicenter retrospective study of inpatients from eleven hospitals in the Infectious Diseases Surveillance of Pediatrics (ISPED) group of China was performed over a two-year period (2018–2019). Statistical significance was calculated by applying the χ2 test or by Fisher’s exact test in the case of small sample sizes. Results A total 220 patients were included. Among included cases, 67 (30.45%) were invasive MRSA infections, including two deaths (2.99%), while 153 (69.55%) were noninvasive infections. The invasive infections of MRSA occurred at a median age of 8 days on admission, which was significantly younger compared to 19 days in noninvasive cases. Sepsis (86.6%) was the most common invasive infection, followed by pneumonia (7.4%), bone and joint infections (3.0%), central nervous system infection (1.5%), and peritonitis (1.5%). Congenital heart disease, low birth weight infant (
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- 2023
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4. Antimicrobial resistance profile of methicillin-resistant Staphylococcus aureus isolates in children reported from the ISPED surveillance of bacterial resistance, 2016–2021
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Xia Wu, Chuanqing Wang, Leiyan He, Hongmei Xu, Chunmei Jing, Yinghu Chen, Aiwei Lin, Jikui Deng, Qing Cao, Huiling Deng, Huijun Cai, Yiping Chen, Jinhong Yang, Ting Zhang, Yuanyuan Huang, Jianhua Hao, and Hui Yu
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methicillin-resistant Staphylococcus aureus ,antimicrobial resistance ,children ,infectious disease surveillance of pediatrics (ISPED) ,neonates ,Microbiology ,QR1-502 - Abstract
IntroductionMethicillin-resistant Staphylococcus aureus (MRSA) poses a serious threat to public health worldwide. In December 2015, the Infectious Disease Surveillance of Pediatrics (ISPED) program was organized to monitor bacterial epidemiology and resistance trends in children.MethodsThis retrospective study was conducted from January 2016–December 2021 on patients at eleven ISPED-group hospitals.ResultsFrom 2016–2021, a total of 13024 MRSA isolates were obtained from children. The most common age group for patients with MRSA infection was less than 3 years old, and newborns were an important group affected by MRSA infection. MRSA was most commonly isolated from the lower respiratory, an abscess, a secretion, or blood in neonates and from the lower respiratory, an abscess, or the upper respiratory in non-neonates. All isolates were susceptible to vancomycin and linezolid and resistant to penicillin; additionally, 76.88%, 54.97%, 22.30%, 5.67%, 5.14%, 3.63%, and 1.42% were resistant to erythromycin, clindamycin, tetracycline, levofloxacin, sulfamethoxazole-trimethoprim (TMP-SMX), gentamicin, and rifampin, respectively. Between 2016 and 2021, a significant increase was seen in the levofloxacin- and TMP-SMX-resistance rates (from 5.45% to 7.14% and from 4.67% to 6.50%, respectively) among MRSA isolates, along with a significant decrease in the rates of resistance to erythromycin (from 82.61% to 68.08%), clindamycin (from 60.95% to 46.82%), tetracycline (from 25.37% to 17.13%), gentamicin (from 4.53% to 2.82%), and rifampin (from 1.89% to 0.41%).DiscussionThe antibiotic-resistance rates varied among MRSA isolated from different sources. Because of the high antibiotic resistance rate to clindamycin, this antibiotic is not recommended for empirical treatment of MRSA infections, especially in osteomyelitis.
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- 2023
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5. Improved targeting of the 16S rDNA nanopore sequencing method enables rapid pathogen identification in bacterial pneumonia in children
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Yinghu Chen, Lingfeng Mao, Dengming Lai, Weize Xu, Yuebai Zhang, Sihao Wu, Di Yang, Shaobo Zhao, Zhicong Liu, Yi Xiao, Yi Tang, Xiaofang Meng, Min Wang, Jueliang Shi, Qixing Chen, and Qiang Shu
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nanopore sequencing ,16S rDNA ,bacterial pneumonia ,BALF ,prospective study ,Microbiology ,QR1-502 - Abstract
ObjectivesTo develop a rapid and low-cost method for 16S rDNA nanopore sequencing.MethodsThis was a prospective study on a 16S rDNA nanopore sequencing method. We developed this nanopore barcoding 16S sequencing method by adding barcodes to the 16S primer to reduce the reagent cost and simplify the experimental procedure. Twenty-one common pulmonary bacteria (7 reference strains, 14 clinical isolates) and 94 samples of bronchoalveolar lavage fluid from children with severe pneumonia were tested. Results indicating low-abundance pathogenic bacteria were verified with the polymerase chain reaction (PCR). Further, the results were compared with those of culture or PCR.ResultsThe turnaround time was shortened to 6~8 hours and the reagent cost of DNA preparation was reduced by employing a single reaction adding barcodes to the 16S primer in advance. The accuracy rate for the 21 common pulmonary pathogens with an abundance ≥ 99% was 100%. Applying the culture or PCR results as the gold standard, 71 (75.5%) of the 94 patients were positive, including 25 positive cultures (26.6%) and 52 positive quantitative PCRs (55.3%). The median abundance in the positive culture and qPCR samples were 29.9% and 6.7%, respectively. With an abundance threshold increase of 1%, 5%, 10%, 15% and 20%, the test sensitivity decreased gradually to 98.6%, 84.9%, 72.6%, 67.1% and 64.4%, respectively, and the test specificity increased gradually to 33.3%, 71.4%, 81.0%, 90.5% and 100.0%, respectively.ConclusionsThe nanopore barcoding 16S sequencing method can rapidly identify the pathogens causing bacterial pneumonia in children.
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- 2023
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6. Clinical features and epidemiological analysis of respiratory human adenovirus infection in hospitalized children: a cross-sectional study in Zhejiang
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Caiyun Wang, Juanjuan Liu, Yumei Mi, Jing Chen, Jing Bi, and Yinghu Chen
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Human adenovirus ,Acute respiratory infections ,Molecular epidemiology ,Children ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background HAdV is one of the common pathogens in hospitalized children with acute respiratory infections (ARIs). We aim to describe the clinical and laboratory features, epidemiological characteristics, and HAdV species and/or types of inpatients with HAdV respiratory infections. Methods Respiratory samples were gathered from inpatients diagnosed ARIs in Children’s Hospital, Zhejiang University School of Medicine, and were detected by using Direct Immunofluorescence Assay from 2018 to 2019. PCR amplification and sequencing of the hypervariable zone of hexon gene were used for genotyping. The clinical and laboratory features, and HAdV genotyping, and epidemiological characteristic analysis were retrospectively performed. Results Of 7072 samples collected, 488 were identified as HAdV-positive. The overall detection rate was 6.9%. The peaked detection rate was 14.1% in January 2019. HAdV-positive cases with ARIs mainly appeared in winter. The detection rate was highest among children between 6 months and 2 years (8.7%, 123/1408). Clinical diagnosis included pneumonia (70.3%, 343/488), bronchitis (7.0%, 34/488) and acute upper respiratory tract infection (22.7%, 111/488). The common clinical manifestations were fever (93.4%, 456/488), cough (94.7%, 462/488), wheezing (26.2%, 128/488), and shortness of breath (14.8%, 72/488). 213 (43.6%) cases had co-infection and 138 (28.3%) cases had extrapulmonary symptoms. 96(19.7%) cases had intrapulmonary and intrathoracic complications.78 (16.0%) had an underlying condition, most of which were congenital heart diseases (20.5%, 16/78). The proportions of hyperpyrexia, duration of fever > 10 days, severe pneumonia, and wheezing in the co-infection group were remarkably higher than those in HAdV single-infection group (all p 10 days, wheezing, shortness of breath, change in level of consciousness, serosal fluids, extrapulmonary symptoms, co-infections and underlying diseases were significantly higher in severe pneumonia group than those in the mild pneumonia group (all p
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- 2021
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7. The low contagiousness and new A958D mutation of SARS-CoV-2 in children: An observational cohort study.
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Yinghu chen, Hangping Yao, Junfen Fu, Qiang Shu, Zhimin Chen, Nanping Wu, Sheng Ye, Wei Wang, Yan Ni, Shiqiang Shang, Wei Li, Jishan Zheng, Shibo Li, Liang Hong, Qi Zhang, Weize Xu, Junsong Chen, Lingyan Fan, Xiaohui Cang, Jianbing Wang, Xiangyun Lu, and Qingyi Cao
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Contagiousness ,Pediatrics ,SARS-CoV-2 ,Evolutionary tree ,New A958D mutation ,Infectious and parasitic diseases ,RC109-216 - Abstract
Aims: To explore the contagiousness and new SARS-CoV-2 mutations in pediatric COVID-19.Methods: This cohort study enrolled all pediatric patients admitted to 8 hospitals in Zhejiang Province of China between 21 January and 29 February 2020, their family members and close-contact classmates. Epidemiological, demographic, clinical and laboratory data were collected. Bioinformatics was used to analyze the features of SARS-CoV-2. Individuals were divided into 3 groups by the first-generation case: Groups 1 (unclear), 2 (adult), and 3 (child). The secondary attack rate (SAR) and R0 were compared among the groups.Results: The infection rate among 211 individuals was 64% (135/211). The SAR in Groups 2 and 3 was 71% (73/103) and 3% (1/30), respectively; the median R0 in Groups 2 and 3 was 2 (range: 1-8) and 0 (range: 0-1), respectively. Compared with adult cases, the SAR and R0 of pediatric cases were significantly lower (p
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- 2021
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8. Bacterial Epidemiology and Antimicrobial Resistance Profiles in Children Reported by the ISPED Program in China, 2016 to 2020
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Pan Fu, Hongmei Xu, Chunmei Jing, Jikui Deng, Hongmei Wang, Chunzhen Hua, Yinghu Chen, Xuejun Chen, Ting Zhang, Hong Zhang, Yiping Chen, Jinhong Yang, Aiwei Lin, Shifu Wang, Qing Cao, Xing Wang, Huiling Deng, Sancheng Cao, Jianhua Hao, Wei Gao, Yuanyuan Huang, Hui Yu, and Chuanqing Wang
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bacteria ,antimicrobial resistance ,children ,Infectious Disease Surveillance of Pediatrics (ISPED) ,multidrug-resistant organisms ,Microbiology ,QR1-502 - Abstract
ABSTRACT The Infectious Disease Surveillance of Pediatrics (ISPED) program was established in 2015 to monitor and analyze the trends of bacterial epidemiology and antimicrobial resistance (AMR) in children. Clinical bacterial isolates were collected from 11 tertiary care children’s hospitals in China in 2016 to 2020. Antimicrobial susceptibility testing was carried out using the Kirby-Bauer method or automated systems, with interpretation according to the Clinical and Laboratory Standards Institute 2019 breakpoints. A total of 288,377 isolates were collected, and the top 10 predominant bacteria were Escherichia coli, Streptococcus pneumoniae, Staphylococcus aureus, Haemophilus influenzae, Klebsiella pneumoniae, Moraxella catarrhalis, Streptococcus pyogenes, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Acinetobacter baumannii. In 2020, the coronavirus disease 2019 (COVID-19) pandemic year, we observed a significant reduction in the proportion of respiratory tract samples (from 56.9% to 44.0%). A comparable reduction was also seen in the primary bacteria mainly isolated from respiratory tract samples, including S. pneumoniae, H. influenzae, and S. pyogenes. Multidrug-resistant organisms (MDROs) in children were commonly observed and presented higher rates of drug resistance than sensitive strains. The proportions of carbapenem-resistant K. pneumoniae (CRKP), carbapenem-resistant A. baumannii (CRAB), carbapenem-resistant P. aeruginosa (CRPA), and methicillin-resistant S. aureus (MRSA) strains were 19.7%, 46.4%%, 12.8%, and 35.0%, respectively. The proportions of CRKP, CRAB, and CRPA strains all showed decreasing trends between 2015 and 2020. Carbapenem-resistant Enterobacteriaceae (CRE) and CRPA gradually decreased with age, while CRAB showed the opposite trend with age. Both CRE and CRPA pose potential threats to neonates. MDROs show very high levels of AMR and have become an urgent threat to children, suggesting that effective monitoring of AMR and antimicrobial stewardship among children in China are required. IMPORTANCE AMR, especially that involving multidrug-resistant organisms (MDROs), is recognized as a global threat to human health; AMR renders infections increasingly difficult to treat, constituting an enormous economic burden and producing tremendous negative impacts on patient morbidity and mortality rates. There are many surveillance programs in the world to address AMR profiles and MDRO prevalence in humans. However, published studies evaluating the overall AMR rates or MDRO distributions in children are very limited or are of mixed quality. In this study, we showed the bacterial epidemiology and resistance profiles of primary pathogens in Chinese children from 2016 to 2020 for the first time, analyzed MDRO distributions with time and with age, and described MDROs’ potential threats to children, especially low-immunity neonates. Our study will be very useful to guide antiinfection therapy in Chinese children, as well as worldwide pediatric patients.
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- 2021
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9. Fas signaling-mediated TH9 cell differentiation favors bowel inflammation and antitumor functions
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Yingying Shen, Zhengbo Song, Xinliang Lu, Zeyu Ma, Chaojie Lu, Bei Zhang, Yinghu Chen, Meng Duan, Lionel Apetoh, Xu Li, Jufeng Guo, Ying Miao, Gensheng Zhang, Diya Yang, Zhijian Cai, and Jianli Wang
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Science - Abstract
Fas signalling induces apoptosis of activated T cells to maintain immune homeostasis. Here the authors show that Fas also induces PKC-β activation to promote NF-κB-mediated TH9 cell differentiation, while p38 activation by PKC-β antagonizes this effect, thereby supporting a synergy between p38 inhibitor and Fas for TH9 differentiation.
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- 2019
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10. Self-adaptive Wi-Fi indoor positioning model.
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Yinghu Chen, Danhuai Guo, Wenjuan Cui, and Jianhui Li
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- 2015
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11. Energy Efficiency Analysis of Data Center Based on All-Inverter Coupled Air-Side Free Cooling Technology
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Chuanxian Ai, Yinghu Chen, and Liang Zhang
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- 2023
12. The low contagiousness and new A958D mutation of SARS-CoV-2 in children: An observational cohort study
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Xiangyun Lu, Wei Li, Qingyi Cao, Shibo Li, Jishan Zheng, Junsong Chen, Qiang Shu, Yan Ni, Xiaohui Cang, Liang Hong, Jianbing Wang, Qi Zhang, Lingyan Fan, Zhi-Min Chen, Hangping Yao, Sheng Ye, Yinghu Chen, Wei Wang, Weize Xu, Junfen Fu, Nanping Wu, and Shiqiang Shang
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Adult ,Microbiology (medical) ,China ,medicine.medical_specialty ,Contagiousness ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,New A958D mutation ,Infectious and parasitic diseases ,RC109-216 ,Pediatrics ,Article ,Cohort Studies ,Internal medicine ,Throat ,Epidemiology ,medicine ,Humans ,Transmission risks and rates ,Child ,Feces ,SARS-CoV-2 ,business.industry ,Incidence ,Incidence (epidemiology) ,COVID-19 ,Infant ,Evolutionary tree ,General Medicine ,Infectious Diseases ,medicine.anatomical_structure ,Mutation ,Spike Glycoprotein, Coronavirus ,Mutation (genetic algorithm) ,business ,Cohort study - Abstract
AIMS: To explore the contagiousness and new SARS-CoV-2 mutations in pediatric COVID-19. METHODS: This cohort study enrolled all pediatric patients admitted to 8 hospitals in Zhejiang Province of China between 21 January and 29 February 2020, their family members and close-contact classmates. Epidemiological, demographic, clinical and laboratory data were collected. Bioinformatics was used to analyze the features of SARS-CoV-2. Individuals were divided into 3 groups by the first-generation case: Groups 1 (unclear), 2 (adult), and 3 (child). The secondary attack rate (SAR) and R0 were compared among the groups. RESULTS: The infection rate among 211 individuals was 64% (135/211). The SAR in Groups 2 and 3 was 71% (73/103) and 3% (1/30), respectively; the median R0 in Groups 2 and 3 was 2 (range: 1-8) and 0 (range: 0-1), respectively. Compared with adult cases, the SAR and R0 of pediatric cases were significantly lower (p
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- 2021
13. PD-L1+ exosomes from bone marrow-derived cells of tumor-bearing mice inhibit antitumor immunity
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Jiaoli Wang, Jufeng Guo, Yinghu Chen, Lei Yu, Tianxin Guo, Yan Sun, and Xian Wang
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0301 basic medicine ,T cell ,Immunology ,CD8-Positive T-Lymphocytes ,Exosomes ,Article ,B7-H1 Antigen ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,Bone Marrow ,In vivo ,Cell Line, Tumor ,PD-L1 ,Immune Tolerance ,medicine ,Animals ,Humans ,Immunology and Allergy ,biology ,Chemistry ,In vitro ,Microvesicles ,030104 developmental biology ,Infectious Diseases ,medicine.anatomical_structure ,Cancer research ,biology.protein ,Bone marrow ,CD8 ,030215 immunology - Abstract
Tumors escape immune attack by upregulating the surface expression of PD-L1, which interacts with PD-1 on T cells to activate immune inhibitory signaling. Anti-PD-1 treatments can effectively block this inhibitory signaling and activate antitumor immune responses. However, anti-PD-1 treatment also has a tumor suppressive effect in patients whose tumor cells do not express PD-L1. The underlying mechanisms are poorly defined. Here, we report that exosomes from bone marrow-derived cells (BMDCs) in tumor-bearing mice, but not in healthy mice, carry PD-L1. PD-L1 on these exosomes is biofunctional and can inhibit CD8(+) T cell proliferation and activation in vitro and in vivo. The transfer of exogenous exosomes from BMDCs and the inhibition of the production of endogenous exosomes by BMDCs promote and suppress tumor growth, respectively. PD-L1(+) exosomes from BMDCs can be found in tumor tissues. In addition, exosomes from BMDCs promote tumor metastasis in a PD-L1-dependent manner. Therefore, our results indicate that exosomes from BMDCs play important roles in tumor immunosuppression via PD-L1.
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- 2020
14. Genome mining and homologous comparison strategy for digging exporters contributing self-resistance in natamycin-producing Streptomyces strains
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Yinghu Chen, Dong Guo, Yi-Ming Shan, Wenjun Guan, Quanshu Gu, Yudong Li, and Yong-Quan Li
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Natamycin ,Mutant ,ATP-binding cassette transporter ,Biology ,Applied Microbiology and Biotechnology ,Streptomyces ,03 medical and health sciences ,Drug Resistance, Bacterial ,medicine ,Data Mining ,Streptomyces chattanoogensis ,Gene ,030304 developmental biology ,Genetics ,0303 health sciences ,030306 microbiology ,Gene Expression Profiling ,Membrane Transport Proteins ,Genomics ,General Medicine ,biology.organism_classification ,Major facilitator superfamily ,Anti-Infective Agents, Local ,Efflux ,Genome, Bacterial ,Biotechnology ,medicine.drug - Abstract
As antibiotics are always toxic to the antibiotic-producing strains themselves, most Streptomyces strains have evolved several self-resistance mechanisms, among which the antibiotic efflux system is understood best and is commonly found. Among the efflux systems, the ATP-binding cassette (ABC) transporter superfamily and the major facilitator superfamily (MFS) are two important transporter families. In this work, the ABC transporters and the MFS transporters from the four reported natamycin-producing Streptomyces strains have been investigated in order to clarify whether these Streptomyces strains share similar efflux strategies for natamycin metabolism. Fifty-one groups of homologous exporter genes were identified as shared by four strains. Differential transcriptional analysis between the natamycin-producing strain Streptomyces chattanoogensis L10 and its ΔscnS0 mutant, which produces no natamycin, reveals that the expression levels of 25 of the above groups of genes were observably changed. The production of natamycin declined over 30% after solely knocking out several of these 25 groups of genes in S. chattanoogensis L10. This indicates that these transporters participate in the efflux of molecules related to natamycin biosynthesis. Our study is the first to demonstrate that the exporters participating in a particular antibiotic metabolism can be excavated and identified quickly by the strategy of genome mining and homologous comparison in the antibiotic-producing strains, leading to deeper understanding of the complex self-resistance mechanisms in Streptomycetes.
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- 2019
15. Multidisciplinary treatment of chronic active Epstein-Barr virus infection with multiple complications: a case report
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Juanjuan Liu, Shiqiang Shang, Ahmed Faisal Ali, and Yinghu Chen
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Pediatrics ,medicine.medical_specialty ,Hereditary pancreatitis ,business.industry ,Hepatosplenomegaly ,Case Report ,medicine.disease ,Recurrent pancreatitis ,Pediatrics, Perinatology and Child Health ,Pancreatic mass ,Etiology ,Medicine ,Pancreatitis ,medicine.symptom ,Differential diagnosis ,business ,Autoimmune pancreatitis - Abstract
Chronic active Epstein-Barr virus (CAEBV) infection is a very rare and potentially life-threatening illness caused by long-term EBV infection. Globally, the prevalence is the highest among young children and adolescents with increased frequency in Asians and Native Americans, whereas it is sporadically encountered in European countries. Typically, patients present with nonspecific symptoms such as fever, lymphadenopathy, hepatosplenomegaly, and liver dysfunction. However, the complications of CAEBV and its treatment are quite complex and require great care. We report a case of a 3-year-old boy with CAEBV infection who was later diagnosed with pancreatic mass and recurrent pancreatitis. A multidisciplinary board was consulted for correct diagnosis and treatment plan making. The treatment included pharmaceutical and surgical (duodenum-preserving pancreatic head resection) approaches. The patient showed tremendous improvement following the third discharge from the hospital and is still free of any symptoms. In this case report, we discuss differential diagnosis and comprehensively examined the possibility of the development of pancreatitis caused by EBV infection, the possibility of autoimmune pancreatitis, and the possibility of hereditary pancreatitis. To confirm or rule out the first two etiologies, laboratory and pathology results were studied. We also performed exon sequencing using Agilent exome capture kit to rule out hereditary pancreatitis. The clinical course of this disease and the way it was handled deserves attention so that similar cases receive prompt treatment.
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- 2021
16. Molecular Epidemiology and clinical Features Analysis of Respiratory Human Adenovirus Infections in Hospitalized Children: A Retrospective Study in Zhejiang
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Yinghu Chen, Jing Chen, Caiyun Wang, Juanjuan Liu, Jing Bi, and Yumei Mi
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medicine.medical_specialty ,Molecular epidemiology ,business.industry ,Internal medicine ,medicine ,virus diseases ,Retrospective cohort study ,Respiratory system ,business ,eye diseases - Abstract
Background: HAdV is one of the common pathogens in hospitalized children with respiratory infection (RI). we aim to describe the epidemiological characteristics, clinical features, and genotype of inpatients with HAdV positive with RI.Methods: Respiratory samples were gathered from inpatients diagnosed RI in Children’s Hospital, Zhejiang University School of Medicine and were detected by using Direct Immunofluorescence Assay (DFA) from 2018 to 2019. PCR amplification and sequencing of the hypervariable zone of hexon gene were used for genotyping. The clinical and laboratory features, and typing of HAdV, and epidemiological characteristic analysis were retrospectively performed. Results: Of 7072 samples collected, 488 cases were identified as HAdV-positive RI. The overall detection rate was 6.9%. The peaked detection rate was 14.1% in January 2019. HAdV-positive cases with RI mainly appeared in winter and summer. The detection rate was highest among children between 6 months and 2 years (8.7%, 123/1408). Clinical diagnosis included pneumonia (70.3%, 343/488), bronchitis (7.0%, 34/488) and acute upper respiratory tract infection (22.7%, 111/488). The common clinical manifestations were fever (93.4%, 456/488), cough (94.7%, 462/488), wheezing (26.2%,128/48), and shortness of breath (14.8%, 72/488). 213 (43.6%) cases had co-infection and 138 (28.3%) cases had extrapulmonary symptoms. 96(19.7%) cases had intrapulmonary and intrathoracic complications.78 (16.0%) had an underlying condition, the most of them were congenital heart diseases (20.5%,17/78). The proportions of hyperpyrexia, duration of fever more than 10 days, severe pneumonia, and proportion of wheezing in the co-infection group were remarkably higher than those in HAdV single-infection group (pp=0.000), while, HAdV-B55 was detected only in the severe group. HAdV-B7’s detection rate in the severe group was significantly higher than the non-severe infected group. Conclusion: HAdV detection rate is related to age and season. Bronchopneumonia accounts for about 70% HAdV -positive inpatients. The common manifestations include hyperpyrexia, cough, wheezing, and shortness of breath. HAdV-B3 and HAdV-B7 are the most common types in children diagnosed with RI.
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- 2021
17. Fas signaling-mediated TH9 cell differentiation favors bowel inflammation and antitumor functions
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Zeyu Ma, Jianli Wang, Diya Yang, Yingying Shen, Gensheng Zhang, Xinliang Lu, Song Zhengbo, Meng Duan, Yinghu Chen, Zhijian Cai, Jufeng Guo, Lionel Apetoh, Lu Chaojie, Bei Zhang, Xu Li, and Ying Miao
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0301 basic medicine ,Science ,T cell ,p38 mitogen-activated protein kinases ,Cellular differentiation ,Cell ,General Physics and Astronomy ,Inflammation ,02 engineering and technology ,Inflammatory bowel disease ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,medicine ,lcsh:Science ,Multidisciplinary ,Chemistry ,General Chemistry ,021001 nanoscience & nanotechnology ,medicine.disease ,030104 developmental biology ,medicine.anatomical_structure ,Apoptosis ,Cancer research ,lcsh:Q ,Signal transduction ,medicine.symptom ,0210 nano-technology - Abstract
Fas induces apoptosis in activated T cell to maintain immune homeostasis, but the effects of non-apoptotic Fas signaling on T cells remain unclear. Here we show that Fas promotes TH9 cell differentiation by activating NF-κB via Ca2+-dependent PKC-β activation. In addition, PKC-β also phosphorylates p38 to inactivate NFAT1 and reduce NFAT1-NF-κB synergy to promote the Fas-induced TH9 transcription program. Fas ligation exacerbates inflammatory bowel disease by increasing TH9 cell differentiation, and promotes antitumor activity in p38 inhibitor-treated TH9 cells. Furthermore, low-dose p38 inhibitor suppresses tumor growth without inducing systemic adverse effects. In patients with tumor, relatively high TH9 cell numbers are associated with good prognosis. Our study thus implicates Fas in CD4+ T cells as a target for inflammatory bowel disease therapy. Furthermore, simultaneous Fas ligation and low-dose p38 inhibition may be an effective approach for TH9 cell induction and cancer therapy.
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- 2019
18. Enhanced Triacylglycerol Metabolism Contributes to Efficient Oil Utilization and High-Level Production of Salinomycin in Streptomyces albus ZD11
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Yong-Quan Li, Jiaxiu Wei, Jianxin Dong, Yinghu Chen, Han Li, Wenjun Guan, and Yudong Li
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food.ingredient ,Fatty acid degradation ,Applied Microbiology and Biotechnology ,Streptomyces ,Soybean oil ,03 medical and health sciences ,Polyketide ,chemistry.chemical_compound ,food ,Lipase ,Triglycerides ,Streptomyces albus ,Salinomycin ,Pyrans ,030304 developmental biology ,0303 health sciences ,Ecology ,biology ,030306 microbiology ,Chemistry ,Metabolism ,biology.organism_classification ,Anti-Bacterial Agents ,Biochemistry ,biology.protein ,Coccidiostats ,Oils ,Food Science ,Biotechnology - Abstract
Streptomyces is well known for biosynthesis of secondary metabolites with diverse bioactivities. Although oils have been employed as carbon sources to produce polyketide antibiotics for several industrial Streptomyces strains, the intrinsic correlation between oil utilization and high production of antibiotics still remains unclear. In this study, we investigated the correlation between oil metabolism and salinomycin biosynthesis in Streptomyces albus ZD11, which employs soybean oil as the main carbon source. Comparative genomic analysis revealed the enrichment of genes related to triacylglycerol (TAG) metabolism in S. albus ZD11. Transcriptomic profiling further confirmed the enhancement of TAG metabolism and acyl coenzyme A biosynthesis in S. albus ZD11. Multiple secreted lipases, which catalyze TAG hydrolysis, were seen to be working in a synergistic and complementary manner in aiding the efficient and stable hydrolyzation of TAGs. Together, our results suggest that enhanced TAG hydrolysis and fatty acid degradation contribute to the high efficiency of oil utilization in S. albus ZD11 in order to provide abundant carbon precursors for cell growth and salinomycin biosynthesis. IMPORTANCE In order to obtain high-level production of antibiotics, oils have been used as the main carbon source for some Streptomyces strains. Based on multiomics analysis, this study provides insight into the relationship between triacylglycerol (TAG) metabolism and antibiotic biosynthesis in S. albus ZD11, an oil-preferring industrial Streptomyces strain. Our investigation into TAG hydrolysis yielded further evidence that this strain utilizes complicated strategies enabling an efficient TAG metabolism. In addition, a novel secreted lipase was identified that exhibited highly hydrolytic activity for medium- and long-chain TAGs. Our findings represent a good start toward clarifying the complicated relationship between TAG catabolism and high-level antibiotic production in the industrial strains.
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- 2020
19. Exosomes from heat-stressed tumour cells inhibit tumour growth by converting regulatory T cells to Th17 cells via IL-6
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Yinghu Chen, Lei Yu, Danfeng Guo, Yingying Shen, Yunshan Yang, Haijun Zhong, and Shoujie Wang
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Adult ,Male ,Time Factors ,Cellular differentiation ,Immunology ,chemical and pharmacologic phenomena ,Adenocarcinoma ,Exosomes ,T-Lymphocytes, Regulatory ,Peripheral blood mononuclear cell ,03 medical and health sciences ,Lymphocytes, Tumor-Infiltrating ,0302 clinical medicine ,Immune system ,Cell Line, Tumor ,Heat shock protein ,Tumor Microenvironment ,Animals ,Humans ,Immunology and Allergy ,Cell Proliferation ,biology ,Interleukin-6 ,Chemistry ,Interleukin ,Cell Differentiation ,Hyperthermia, Induced ,Original Articles ,Transforming growth factor beta ,Middle Aged ,Microvesicles ,In vitro ,Tumor Burden ,Mice, Inbred C57BL ,030220 oncology & carcinogenesis ,Colonic Neoplasms ,Cancer research ,biology.protein ,Th17 Cells ,Female ,Heat-Shock Response ,Signal Transduction ,030215 immunology - Abstract
Exosomes derived from heat‐stressed tumour cells (HS‐TEXs), which contain abundant heat shock protein (HSP) 70, strongly induce antitumour immune responses. HSP70‐induced interleukin (IL)‐6 promotes IL‐17 expression and causes rejection of established prostate tumours. However, it remains unclear whether HS‐TEXs exhibit antitumour effects by converting regulatory T cells (T(regs)) into T helper type 17 (Th17) cells. In this study, we found that compared with TEXs, HS‐TEXs were more potent in stimulating secretion of IL‐6 from dendritic cells. In vitro, IL‐6 blocked tumour cell‐derived transforming growth factor beta 1‐induced T(reg) differentiation and promoted Th17 cell differentiation. HS‐TEXs exerted strong antitumour effects, converting T(regs) into Th17 cells with high efficiency, a process that was entirely dependent upon IL‐6. Neutralization of IL‐17 completely abolished the antitumour effect of TEXs, but only partially inhibited that of HS‐TEXs. In addition, we found higher levels of IL‐6 and IL‐17 in serum from tumour patients treated with hyperthermia, and an increase in Th17 cells and a decrease in T(regs) was detected in peripheral blood mononuclear cells isolated from these patients after hyperthermia. Therefore, our results demonstrate that HS‐TEXs possess a powerful capacity to convert immunosuppressive T(regs) into Th17 cells via IL‐6, which contributes to their potent antitumour effect.
- Published
- 2018
20. High doses of recombinant mannan-binding lectin inhibit the binding of influenza A(H1N1)pdm09 virus with cells expressing DC-SIGN
- Author
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Hao Peng, Ran Tao, Caiyun Wang, Yinghu Chen, Shiqiang Shang, Li Zhang, and Lei Yu
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0301 basic medicine ,Microbiology (medical) ,medicine.drug_class ,viruses ,Virus Attachment ,Receptors, Cell Surface ,chemical and pharmacologic phenomena ,Monoclonal antibody ,Mannose-Binding Lectin ,Monocytes ,Virus ,Pathology and Forensic Medicine ,law.invention ,03 medical and health sciences ,Influenza A Virus, H1N1 Subtype ,0302 clinical medicine ,C-type lectin ,law ,medicine ,Animals ,Humans ,Immunology and Allergy ,Lectins, C-Type ,Cells, Cultured ,Mannan-binding lectin ,biology ,Chemistry ,virus diseases ,Lectin ,Complement System Proteins ,Dendritic Cells ,General Medicine ,Virology ,Recombinant Proteins ,DC-SIGN ,030104 developmental biology ,Cell culture ,biology.protein ,Recombinant DNA ,Receptors, Virus ,Cell Adhesion Molecules ,030215 immunology - Abstract
The pandemic influenza A (H1N1)pdm09 virus continues to be a threat to human health. Low doses of mannan-binding lectin (MBL) (
- Published
- 2017
21. Diagnosis and treatment of herpangina: Chinese expert consensus
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Xing-Dong Wu, Ying-Xue Zou, Xingwang Li, Jin-Zhun Wu, Ying-Zi Ye, Ai-Wei Lin, Xiao-Dong Liu, Jianhua Hao, Quan-Bo Liu, Hui-Ling Deng, Yan Chen, Ru-Ping Luo, Changshan Liu, Gang Liu, Zhao-Bo Shen, Guangmin Nong, Jikui Deng, Bi-Quan Chen, Yinghu Chen, Hui Yu, and Rongmeng Jiang
- Subjects
medicine.medical_specialty ,China ,Herpangina ,Echovirus ,business.industry ,Decision Trees ,Expert consensus ,medicine.disease_cause ,medicine.disease ,Subspecialty ,Diagnosis, Differential ,Infectious disease (medical specialty) ,Pediatrics, Perinatology and Child Health ,Epidemiology ,Pediatric surgery ,medicine ,Enterovirus ,Humans ,Intensive care medicine ,business ,Child - Abstract
Herpangina is a common infectious disease in childhood caused by an enterovirus. This consensus is aiming to standardize and improve herpangina prevention and clinical diagnosis. The Subspecialty Group of Infectious Diseases, the Society of Pediatric, Chinese Medical Association and Nation Medical Quality Control Center for Infectious Diseases gathered 20 experts to develop the consensus, who are specialized in diagnosis and treatment of herpangina. The main pathogenic serotypes of herpangina include Coxsackievirus-A, Enterovirus-A and Echovirus. Its diagnosis can be rendered on the basis of history of epidemiology, typical symptoms, characteristic pharyngeal damage and virological tests. The treatment is mainly symptomatic, and incorporates topical oral spray with antiviral drugs. The course of herpangina generally lasts 4–6 days with a good prognosis. The consensus could provide advices and references for the diagnosis, treatment and management of herpangina in children.
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- 2019
22. Prognostic factors in pediatric pneumococcal meningitis patients in mainland China: a retrospective multicenter study
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Caiyun, Wang, Hongmei, Xu, Jikui, Deng, Hui, Yu, Yiping, Chen, Shifu, Wang, Weichun, Huang, Jianhua, Hao, Chun, Wang, Huiling, Deng, and Yinghu, Chen
- Subjects
pediatric ,complication ,clinical findings ,prognosis ,Original Research ,pneumococcal meningitis - Abstract
Background: Prognosis of pneumococcal meningitis (PM) remains very poor, especially in less developed countries. Currently, few multi-centric studies on pediatric PM have been reported in mainland China. Objectives: This study aimed to explore the correlation of clinical and laboratory findings with complications and prognosis in pediatric PM. Methods: The pediatric PM patients were retrospectively recruited from ten pediatric tertiary hospitals across China between January 2013 and June 2018. Clinical, biochemical, and microbiological data and follow-up information were collected. Predictive factors for complications and prognostic factors for overall survival (OS) and sequelae-free survival (SFS) were analyzed. Results: A total of 132 pediatric PM patients were included. Seventy-one patients had complications, 25 patients died, and 39 patients had neurological sequelae. Multivariate logistic regression suggested that age less than 28 months (adjusted OR=2.654, 95%CI=1.067–6.600, P=0.036) and lower white blood cells in blood (aOR=3.169, 95%CI=1.395–7.202, P=0.006) were associated with high risk of complications. Multivariate Cox’s proportional hazard regression suggested that age less than 28 months (aHR=6.479, 95%CI=1.153–36.404, P=0.034), coma (aHR=9.808, 95%CI=2.802–34.323, P=0.000), and non-adjuvant steroid therapy (aHR=4.768 95%CI=1.946–11.678, P=0.001) were independent prognostic factors for poor OS; coma (aHR=5.841, 95%CI=2.652–12.864, P=0.000), septic shock on admission (aHR=2.949, 95%CI=1.049–8.290, P=0.040), and lower glucose level in cerebrospinal fluid (CSF) (aHR=2.523, 95%CI=1.336–4.765, P=0.004) were independent prognostic factors for poor SFS. Conclusion: Age, coma, and adjuvant steroid therapy were independent factors for OS, while coma, septic shock on admission, and lower glucose level in CSF were independent factors for SFS in pediatric PM patients. These factors might be used to identify PM patients with poor prognosis and guide individual treatment.
- Published
- 2018
23. CD38 gene-modified dendritic cells inhibit murine asthma development by increasing IL-12 production and promoting Th1 cell differentiation
- Author
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Zhendong Lin, Yinghu Chen, Jiaoli Wang, Weiguo Zhu, and Shenglin Ma
- Subjects
Lipopolysaccharides ,0301 basic medicine ,Cancer Research ,Cellular differentiation ,Cell ,Immunoglobulin E ,Biochemistry ,Mice ,03 medical and health sciences ,Th2 Cells ,0302 clinical medicine ,immune system diseases ,Interferon ,Genetics ,medicine ,Animals ,Humans ,Th1-Th2 Balance ,Molecular Biology ,Membrane Glycoproteins ,biology ,Cluster of differentiation ,Cell Polarity ,Granulocyte-Macrophage Colony-Stimulating Factor ,Interleukin ,Cell Differentiation ,Dendritic Cells ,Th1 Cells ,Cell cycle ,ADP-ribosyl Cyclase 1 ,Interleukin-12 ,Asthma ,Disease Models, Animal ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,Immunology ,biology.protein ,Interleukin 12 ,Molecular Medicine ,Interleukin-4 ,Bronchoalveolar Lavage Fluid ,030215 immunology ,medicine.drug - Abstract
Predominant T helper (Th)2 and impaired Th1 cell polarization has a crucial role in the development of asthma. Cluster of differentiation (CD)38 is associated with the increased release of interleukin (IL)‑12 from dendritic cells (DCs) and DC‑induced Th1 cell polarization. However, whether CD38 expression affects DC function in asthma development remains unknown. In the current study, adenoviruses were constructed containing the murine CD38 gene. Overexpression of CD38 protein level in DCs induced from bone‑marrow derived DCs (BMDCs) by recombinant mouse granulocyte macrophage colony‑stimulating factor and IL‑4 was achieved through 24 h adenovirus infection. The results demonstrated that BMDCs with CD38 overexpression exhibited no phenotypic change; however, following stimulation with lipopolysaccharide (LPS), maturation and IL‑12 secretion were increased. In addition, CD38‑overexpressing BMDCs stimulated with LPS exhibited more effective Th1 cell differentiation. Mice that were administered CD38‑overexpressing BMDCs exhibited milder symptoms of asthma. Furthermore, decreased IL‑4, IL‑5 and IL‑13 levels were detected in bronchoalveolar lavage fluid (BALF), reduced immunoglobulin E levels were measured in the sera, and increased interferon‑γ was detected in BALF from the recipients of CD38‑overexpressing BMDCs. Increased phosphorylated‑p38 expression was also detected in LPS-stimulated CD38-overexpressing BMDCs, whereas pretreatment with a p38‑specific inhibitor was able to abolish the effects of LPS stimulation and CD38 overexpression on IL‑12 release and Th1 cell differentiation in BMDCs. These results suggested that CD38 may be involved in the DC function of alleviating asthma via restoration of the Th1/Th2 balance, thus providing a novel strategy for asthma therapy.
- Published
- 2016
24. Fas signaling-mediated T
- Author
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Yingying, Shen, Zhengbo, Song, Xinliang, Lu, Zeyu, Ma, Chaojie, Lu, Bei, Zhang, Yinghu, Chen, Meng, Duan, Lionel, Apetoh, Xu, Li, Jufeng, Guo, Ying, Miao, Gensheng, Zhang, Diya, Yang, Zhijian, Cai, and Jianli, Wang
- Subjects
Mice, Inbred BALB C ,NFATC Transcription Factors ,NF-kappa B ,Mice, Nude ,Cell Differentiation ,Cancer immunotherapy ,Signal transduction ,Inflammatory Bowel Diseases ,T-Lymphocytes, Regulatory ,p38 Mitogen-Activated Protein Kinases ,Article ,Mice, Inbred C57BL ,Mice ,Lymphocyte differentiation ,Neoplasms ,Protein Kinase C beta ,Animals ,Cytokines ,Humans ,Female ,fas Receptor ,CD4-positive T cells - Abstract
Fas induces apoptosis in activated T cell to maintain immune homeostasis, but the effects of non-apoptotic Fas signaling on T cells remain unclear. Here we show that Fas promotes TH9 cell differentiation by activating NF-κB via Ca2+-dependent PKC-β activation. In addition, PKC-β also phosphorylates p38 to inactivate NFAT1 and reduce NFAT1-NF-κB synergy to promote the Fas-induced TH9 transcription program. Fas ligation exacerbates inflammatory bowel disease by increasing TH9 cell differentiation, and promotes antitumor activity in p38 inhibitor-treated TH9 cells. Furthermore, low-dose p38 inhibitor suppresses tumor growth without inducing systemic adverse effects. In patients with tumor, relatively high TH9 cell numbers are associated with good prognosis. Our study thus implicates Fas in CD4+ T cells as a target for inflammatory bowel disease therapy. Furthermore, simultaneous Fas ligation and low-dose p38 inhibition may be an effective approach for TH9 cell induction and cancer therapy., Fas signalling induces apoptosis of activated T cells to maintain immune homeostasis. Here the authors show that Fas also induces PKC-β activation to promote NF-κB-mediated TH9 cell differentiation, while p38 activation by PKC-β antagonizes this effect, thereby supporting a synergy between p38 inhibitor and Fas for TH9 differentiation.
- Published
- 2018
25. The influence of HCMV infection on autophagy in THP-1 cells
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Jianwei Pan, Wei Li, Ran Tao, Huamei Li, Yujie Liu, Yinghu Chen, Shiqiang Shang, and Lifang Liu
- Subjects
0301 basic medicine ,Human cytomegalovirus ,autophagy ,ATG5 ,Cell Culture Techniques ,Cytomegalovirus ,Cell Line ,03 medical and health sciences ,Western blot ,Medicine ,Humans ,THP1 cell line ,HCMV ,latency ,medicine.diagnostic_test ,business.industry ,Autophagy ,General Medicine ,Clinical Trial/Experimental Study ,medicine.disease ,Virology ,infection ,Virus Latency ,Reverse transcription polymerase chain reaction ,Leukemia, Myeloid, Acute ,030104 developmental biology ,Viral replication ,Cell culture ,Cytomegalovirus Infections ,THP-1 ,business ,Research Article - Abstract
Background: Human cytomegalovirus (HCMV) infection is very common and latency can be reactivated in the future. And it can alter the intracellular environment, similar to other herpesviruses, for viral replication and survival. The aim of this study was to investigate the influence of HCMV infection on autophagy in human acute monocytic leukemia cell line (THP-1 cells). Methods: Reverse transcription polymerase chain reaction (RT-PCR), western blot, and transmission electron microscope (TEM) were used to examine autophagy level. The concentrations of autophagy-related proteins Beclin 1, Atg5, and the light chain three (LC3) were counted when compared with actin level. Results: The expression levels of Beclin1, Atg5, and LC3II mRNAs increased gradually between 1 and 5 days(d) postinfection (p.i.) and subsequently decreased little by little when compared with the control THP-1 cells. However, results of western blot analysis displayed that the level of LC3II increased gradually after 1 day p.i. and decreased at 7 days after infection. But the levels of Atg5 and Beclin1 increased gradually after 2 days p.i. and began to decrease at 5 days after infection, respectively. Conclusion: These results suggested that HCMV infection can facilitate the autophagy and autophagy level may decrease in latent phase. More studies on the relationship between HCMV latency and autophagy are needed to determine the role of autophagy in HCMV latent infection that may help find out a therapeutic approach for clinical treatment.
- Published
- 2017
26. Specific Decrease in B-Cell-Derived Extracellular Vesicles Enhances Post-Chemotherapeutic CD8+ T Cell Responses
- Author
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Lei Yu, Lu Chaojie, Jianli Wang, Fanghui Zhang, Wu Zhou, Zhijian Cai, Yunshan Yang, Yinghu Chen, Li Rongrong, Wanjing Zhang, Aifu Lin, Yingying Shen, Zhenwei Xue, and Chunhui Shi
- Subjects
0301 basic medicine ,biology ,Immunology ,Nod ,Adenosine ,CD19 ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Infectious Diseases ,medicine.anatomical_structure ,Downregulation and upregulation ,030220 oncology & carcinogenesis ,biology.protein ,medicine ,Cancer research ,Immunology and Allergy ,Gene silencing ,Cytotoxic T cell ,B cell ,CD8 ,medicine.drug - Abstract
Summary Systemic immunosuppression greatly affects the chemotherapeutic antitumor effect. Here, we showed that CD19+ extracellular vesicles (EVs) from B cells through CD39 and CD73 vesicle-incorporated proteins hydrolyzed ATP from chemotherapy-treated tumor cells into adenosine, thus impairing CD8+ T cell responses. Serum CD19+ EVs were increased in tumor-bearing mice and patients. Patients with fewer serum CD19+ EVs had a better prognosis after chemotherapy. Upregulated hypoxia-inducible factor-1α (HIF-1α) promoted B cells to release CD19+ EVs by inducing Rab27a mRNA transcription. Rab27a or HIF-1α deficiency in B cells inhibited CD19+ EV production and improved the chemotherapeutic antitumor effect. Silencing of Rab27a in B cells by inactivated Epstein-Barr viruses carrying Rab27a siRNA greatly improved chemotherapeutic efficacy in humanized immunocompromised NOD Prkdcscid Il2rg−/− mice. Thus, decreasing CD19+ EVs holds high potential to improve the chemotherapeutic antitumor effect.
- Published
- 2019
27. Exosomes with membrane-associated TGF-β1 from gene-modified dendritic cells inhibit murine EAE independently of MHC restriction
- Author
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Xuetao Cao, Jianli Wang, Feng Xu, Yinxiang Wei, Lingling Jiang, Keyi Wang, Yinghu Chen, Zhijian Cai, Lei Yu, and Fei Yang
- Subjects
Adoptive cell transfer ,Proteolipid protein 1 ,biology ,fungi ,Immunology ,food and beverages ,FOXP3 ,macromolecular substances ,Myelin oligodendrocyte glycoprotein ,carbohydrates (lipids) ,Interleukin 10 ,biology.protein ,Immunology and Allergy ,Interleukin 17 ,STAT3 ,Ex vivo - Abstract
We have previously demonstrated that exosomes from dendritic cells (DCs) secreting TGF-β1 (sTGF-β1-EXOs) delay the development of murine inflammatory bowel disease (IBD). In this study, we isolated exosomes from DCs expressing membrane-associated TGF-β1 (mTGF-β1-EXOs) and found mTGF-β1-EXOs had more potent immunosuppressive activity than sTGF-β1-EXOs in vitro. Treatment of mice with mTGF-β1-EXOs inhibited the development and progression of myelin oligodendrocyte glycoprotein (MOG) peptide-induced EAE even after disease onset. Treatment of mice with mTGF-β1-EXOs also impaired Ag-specific Th1 and IL-17 responses, but promoted IL-10 responses ex vivo. Treatment with mTGF-β1-EXOs decreased the frequency of Th17 cells in EAE mice, which might be associated with the down-regulation of the p38, ERK, Stat3, and NF-κB activation and IL-6 expression in DCs. Treatment with mTGF-β1-EXOs maintained the regulatory capacity of Treg cells, and adoptive transfer of CD4(+)Foxp3(+)Treg cells from mTGF-β1-EXO-treated EAE mice dramatically prevented the development of EAE in the recipients. Moreover, treatment with mTGF-β1-EXOs from C57BL/6 mice effectively prevented and inhibited proteolipid protein (PLP) peptide-induced EAE in BALB/c mice. These results indicate that mTGF-β1-EXOs possess powerful immunosuppressive ability and can effectively inhibit the development and progression of EAE in different strains of mice.
- Published
- 2013
28. Autophagy is involved in regulating the immune response of dendritic cells to influenza A (H1N1) pdm09 infection
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Zhendong Lin, Jiaoli Wang, Feng Xu, Huoquan Lu, Yinghu Chen, Lei Yu, Weiguo Zhu, Zhijian Cai, and Farong Zang
- Subjects
0301 basic medicine ,p38 mitogen-activated protein kinases ,Immunology ,Antigen presentation ,Biology ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Immune system ,Influenza A Virus, H1N1 Subtype ,Antigen ,Orthomyxoviridae Infections ,Interferon ,medicine ,Autophagy ,Immunology and Allergy ,Animals ,Toll-like receptor ,Antigen Presentation ,Mice, Inbred BALB C ,Toll-Like Receptors ,FOXP3 ,Dendritic Cells ,Original Articles ,Cell biology ,Mice, Inbred C57BL ,030104 developmental biology ,Cytokines ,Beclin-1 ,Female ,Apoptosis Regulatory Proteins ,Corrigendum ,030215 immunology ,medicine.drug ,Signal Transduction - Abstract
Autophagy can mediate antiviral immunity. However, it remains unknown whether autophagy regulates the immune response of dendritic cells (DCs) to influenza A (H1N1) pdm09 infection. In this study, we found that infection with the H1N1 virus induced DC autophagy in an endocytosis-dependent manner. Compared with autophagy-deficient Beclin-1(+/-) mice, we found that bone-marrow-derived DCs from wild-type mice (WT BMDCs) presented a more mature phenotype on H1N1 infection. Wild-type BMDCs secreted higher levels of interleukin-6 (IL-6), tumour necrosis factor- α (TNF-α), interferon-β (IFN-β), IL-12p70 and IFN-γ than did Beclin-1(+/-) BMDCs. In contrast to Beclin-1(+/-) BMDCs, H1N1-infected WT BMDCs exhibited increased activation of extracellular signal-regulated kinase, Jun N-terminal kinase, p38, and nuclear factor-κB as well as IFN regulatory factor 7 nuclear translocation. Blockade of autophagosomal and lysosomal fusion by bafilomycin A1 decreased the co-localization of H1N1 viruses, autophagosomes and lysosomes as well as the secretion of IL-6, TNF-α and IFN-β in H1N1-infected BMDCs. In contrast to Beclin-1(+/-) BMDCs, H1N1-infected WT BMDCs were more efficient in inducing allogeneic CD4(+) T-cell proliferation and driving T helper type 1, 2 and 17 cell differentiation while inhibiting CD4(+) Foxp3(+) regulatory T-cell differentiation. Moreover, WT BMDCs were more efficient at cross-presenting the ovalbumin antigen to CD8(+) T cells. We consistently found that Beclin-1(+/-) BMDCs were inferior in their inhibition of H1N1 virus replication and their induction of H1N1-specific CD4(+) and CD8(+) T-cell responses, which produced lower levels of IL-6, TNF-α and IFN-β in vivo. Our data indicate that autophagy is important in the regulation of the DC immune response to H1N1 infection, thereby extending our understanding of host immune responses to the virus.
- Published
- 2016
29. Self-adaptive Wi-Fi indoor positioning model
- Author
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Danhuai Guo, Wenjuan Cui, Jianhui Li, and Yinghu Chen
- Subjects
Artificial neural network ,Computer science ,business.industry ,Attenuation ,Embedded system ,Real-time computing ,Fingerprint (computing) ,Location-based service ,k-means clustering ,Self adaptive ,business ,Representation (mathematics) ,Adaptation (computer science) - Abstract
Wi-Fi based indoor positioning, which is based on attenuation of Received Signal Strength Indicator (RSSI) is an emerging Location Based Service (LBS) technology. As positioning accuracy is sensitive to environmental factors, most of the existing algorithms based on experimental test perform badly without adaptation to dynamics of environment. In this paper, we propose an indoor positioning method by locating the representation of a cluster within similar environments. The K-Means algorithm is used to extract the similarities of the objects within the nearby area. To overcome the problem of parameter determination under the circumstances of lack of fingerprint and extra hardware, we proposed a Log-normal shadowing model (LNSM) with Artificial Neural Networks to estimate distance enabling the parameters to be dynamically adjusted according to the change of the environment. The experimental results of one day auto fair data demonstrate the performance of our method with a higher degree of accuracy than other methods.
- Published
- 2015
30. Increased anti-tumour activity by exosomes derived from doxorubicin-treated tumour cells via heat stress
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Yunshan Yang, Qiyu Zhao, Haijun Zhong, Yinghu Chen, and Fanghui Zhang
- Subjects
Cancer Research ,Physiology ,Apoptosis ,macromolecular substances ,Biology ,Exosomes ,Flow cytometry ,Mice ,Physiology (medical) ,Neoplasms ,polycyclic compounds ,medicine ,Animals ,Humans ,Doxorubicin ,MTT assay ,medicine.diagnostic_test ,Cell growth ,fungi ,food and beverages ,Molecular biology ,Microvesicles ,Heat stress ,carbohydrates (lipids) ,Drug carrier ,Heat-Shock Response ,medicine.drug - Abstract
Tumour-cell-derived exosomes (Exo) have been proposed as a new kind of drug carrier, and heat stress can promote release of exosomes from tumour cells. This study investigated the impact of heat stress on the quantity of doxorubicin in exosomes from the same number of doxorubicin-treated MFC-7 tumour cells and their anti-tumour effects.Exosomes were isolated from phosphate-buffered saline (Exo), doxorubicin (Exo-Dox) or doxorubicin combined with heat-stress-treated (Exo-Dox-HS) MCF-7 cells. The content of doxorubicin in the exosomes was determined by flow cytometry. The effects of individual types of exosomes on the MCF-7 cell proliferation and apoptosis as well as the tumour growth were determined by MTT assay, flow cytometry and murine xenograft tumour modelling.We found that the amount of Exo-Dox-HS was higher than that of Exo-Dox from the same number of MCF-7 cells, and Exo-Dox-HS contained higher levels of doxorubicin than Exo-Dox from the same number of cells. Exo-Dox and Exo-Dox-HS, but not Exo or 10 µg/mL doxorubicin, significantly inhibited the MCF-7 cell proliferation and triggered MCF-7 cell apoptosis, associated with increased levels of cleaved caspase-3 and -8 and morphological changes in MCF-7 cells. Treatment with Exo-Dox and Exo-Dox-HS inhibited the growth of implanted breast tumours in mice.Our study indicated that heat stress increased the quantity of doxorubicin-containing exosomes from tumour cells, and enhanced the anti-tumour effect of exosomes from the doxorubicin-treated tumour cells. Our findings may aid in designing new strategies for cancer therapy by combination of chemotherapy and hyperthermia.
- Published
- 2015
31. A ten-year retrospective review of 1,107 snakebite patients in Sanya, China
- Author
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Jianbo, Shuang, Yinghu, Chen, Qihua, Ran, Xiaoqiang, Liao, Wenbo, Lin, Jianbo, Wu, and Lijie, Li
- Subjects
Adult ,Male ,China ,Young Adult ,Humans ,Snake Bites ,Female ,Middle Aged ,Retrospective Studies - Published
- 2014
32. The influence of HCMV infection on autophagy in THP-1 cells.
- Author
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Yujie Liu, Jianwei Pan, Lifang Liu, Wei Li, Ran Tao, Yinghu Chen, Huamei Li, Shiqiang Shang, Liu, Yujie, Pan, Jianwei, Liu, Lifang, Li, Wei, Tao, Ran, Chen, Yinghu, Li, Huamei, and Shang, Shiqiang
- Published
- 2017
- Full Text
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33. Exosomes with membrane-associated TGF-β1 from gene-modified dendritic cells inhibit murine EAE independently of MHC restriction
- Author
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Lei, Yu, Fei, Yang, Lingling, Jiang, Yinghu, Chen, Keyi, Wang, Feng, Xu, Yinxiang, Wei, Xuetao, Cao, Jianli, Wang, and Zhijian, Cai
- Subjects
STAT3 Transcription Factor ,Encephalomyelitis, Autoimmune, Experimental ,Green Fluorescent Proteins ,Autoimmunity ,Exosomes ,Lymphocyte Activation ,T-Lymphocytes, Regulatory ,p38 Mitogen-Activated Protein Kinases ,Transforming Growth Factor beta1 ,Mice ,Animals ,Extracellular Signal-Regulated MAP Kinases ,Cells, Cultured ,Cell Proliferation ,Immunosuppression Therapy ,Mice, Inbred BALB C ,Interleukin-6 ,Interleukin-17 ,NF-kappa B ,Dendritic Cells ,Th1 Cells ,Inflammatory Bowel Diseases ,Adoptive Transfer ,Interleukin-10 ,Enzyme Activation ,Mice, Inbred C57BL ,Th17 Cells ,Female ,Myelin-Oligodendrocyte Glycoprotein - Abstract
We have previously demonstrated that exosomes from dendritic cells (DCs) secreting TGF-β1 (sTGF-β1-EXOs) delay the development of murine inflammatory bowel disease (IBD). In this study, we isolated exosomes from DCs expressing membrane-associated TGF-β1 (mTGF-β1-EXOs) and found mTGF-β1-EXOs had more potent immunosuppressive activity than sTGF-β1-EXOs in vitro. Treatment of mice with mTGF-β1-EXOs inhibited the development and progression of myelin oligodendrocyte glycoprotein (MOG) peptide-induced EAE even after disease onset. Treatment of mice with mTGF-β1-EXOs also impaired Ag-specific Th1 and IL-17 responses, but promoted IL-10 responses ex vivo. Treatment with mTGF-β1-EXOs decreased the frequency of Th17 cells in EAE mice, which might be associated with the down-regulation of the p38, ERK, Stat3, and NF-κB activation and IL-6 expression in DCs. Treatment with mTGF-β1-EXOs maintained the regulatory capacity of Treg cells, and adoptive transfer of CD4(+)Foxp3(+)Treg cells from mTGF-β1-EXO-treated EAE mice dramatically prevented the development of EAE in the recipients. Moreover, treatment with mTGF-β1-EXOs from C57BL/6 mice effectively prevented and inhibited proteolipid protein (PLP) peptide-induced EAE in BALB/c mice. These results indicate that mTGF-β1-EXOs possess powerful immunosuppressive ability and can effectively inhibit the development and progression of EAE in different strains of mice.
- Published
- 2012
34. Lower mannose-binding lectin contributes to deleterious H1N1 2009 infection in children
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Shiqiang Shang, ChenMei Zhang, Meiqin Tong, Lailong Gao, and Yinghu Chen
- Subjects
Microbiology (medical) ,Male ,viruses ,chemical and pharmacologic phenomena ,Mannose-Binding Lectin ,Virus ,Neutralization ,Pathology and Forensic Medicine ,Microbiology ,Influenza A Virus, H1N1 Subtype ,Influenza, Human ,Immunology and Allergy ,Humans ,Prospective Studies ,Neutralizing antibody ,Child ,Lung ,Mannan-binding lectin ,Innate immune system ,biology ,virus diseases ,Lectin ,General Medicine ,bacterial infections and mycoses ,Immunity, Innate ,Complement system ,Antibody opsonization ,Hospitalization ,Intensive Care Units ,Child, Preschool ,Immunology ,biology.protein ,Female - Abstract
Mannose-binding lectin (MBL) has broad range of activity against viruses through the mechanisms of neutralization, opsonization, and complement activation. Prior studies have demonstrated that MBL inactivated the season's influenza virus. Due to the fact that children have no neutralizing antibody against H1N1 2009 virus, innate immunity may be crucial in the defense against influenza. Therefore, we studied whether MBL levels played a role in H1N1 2009 infection in children. In a prospective survey, we revealed that MBL levels in ICU influenza cases were significantly lower than in children with influenza from infection disease ward. MBL may be involved in innate immune responses to H1N1 2009 infection in children.
- Published
- 2012
35. Clinical features of severe influenza A (H1N1) virus infection
- Author
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Yongmin Tang, Shiqiang Shang, ChenMei Zhang, Yinghu Chen, Yuwen Dai, and Meiqin Tong
- Subjects
Male ,Pediatrics ,medicine.medical_specialty ,ARDS ,Pleural effusion ,Influenza A (H1N1) ,Encephalopathy ,medicine.disease_cause ,Polymerase Chain Reaction ,Influenza A Virus, H1N1 Subtype ,Pandemic ,Influenza, Human ,medicine ,Influenza A virus ,Humans ,Viral shedding ,Retrospective Studies ,Pediatric ,business.industry ,virus diseases ,Infant ,Retrospective cohort study ,medicine.disease ,Pneumothorax ,Fluorescent Antibody Technique, Direct ,Child, Preschool ,Pediatrics, Perinatology and Child Health ,Immunology ,Female ,Original Article ,business - Abstract
Objective To highlight the clinical presentations of influenza A (H1N1) infection, for early diagnosis and recognition by the pediatricians. Methods In this retrospective study, the medical records of inpatients with influenza A (H1N1) infection between November 1, 2009 and May 31, 2011were reviewed. Results Eighty pediatric in-patients with median age 41.9 mo were studied. ARDS (11/80), pneumothorax (8/80), pleural effusion (7/80) and encephalopathy (7/80) were the most frequent complications. Six of 11 ARDS patients died;all of them were under 5 y. The median days of viral shedding was 11.4 d. Slight increase of Il-6, Il-10 and TNF-γ were revealed in some cases. Conclusions During late stage of pandemic wave, the majority of patients were young children. Children with severe Influenza A (H1N1) are prone to develop complications, and die from ARDS. If influenza-like illness is accompanied by neurologic signs, influenza A (H1N1) virus infection should be considered. The viral shedding in children is longer than in adults.
- Published
- 2011
36. Human mannose-binding lectin inhibits human cytomegalovirus infection in human embryonic pulmonary fibroblast
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Ran Tao, Hui-Ju Qiao, Weizhong Gu, Shiqiang Shang, Yinghu Chen, and Wei Wu
- Subjects
Microbiology (medical) ,Human cytomegalovirus ,viruses ,Cytomegalovirus ,chemical and pharmacologic phenomena ,CHO Cells ,Mannose-Binding Lectin ,Virus ,Pathology and Forensic Medicine ,Microbiology ,Viral entry ,Cricetinae ,medicine ,Immunology and Allergy ,Animals ,Humans ,Lung ,Cells, Cultured ,Mannan-binding lectin ,Cytopathic effect ,biology ,virus diseases ,Lectin ,General Medicine ,biochemical phenomena, metabolism, and nutrition ,Fibroblasts ,Virus Internalization ,bacterial infections and mycoses ,medicine.disease ,In vitro ,Recombinant Proteins ,Viral replication ,Cytomegalovirus Infections ,DNA, Viral ,biology.protein - Abstract
A limited number of drugs have been used for treatment of human cytomegalovirus (HCMV), all sharing the similar antiviral mechanism of inhibiting virus replication. This study investigates the anti-HCMV activities of mannose-binding lectin (MBL) from blocking virus entry and inhibiting virus spread. Recombinant human MBL was produced in CHO cells and native human MBL was isolated from human serum. A HCMV neutralization test was performed by pre-treating HCMV with each diluted MBL solution. Then the treated HCMV was inoculated onto the human embryonic pulmonary fibroblasts (HELF), which was followed by HCMV-DNA detection, PP65 positivity examination and confocal imaging of the infected cells. To test the activity of MBL in inhibiting viral spreading after viral invasion, HCMV growth inhibition test was performed. The infected cells were incubated with each diluted MBL, every 24 h, the supernatant was tested for HCMV-DNA. After 72 h, cells were collected for HCMV-DNA and PP65 examination. Then the cytopathic effect was observed and cell viability was measured at the 5 days after infection. HCMV neutralization test revealed 10 μg/mL MBL significantly decreased the HCMV invasion in HELF and the anti-HCMV activity can be blocked by 20 mg/mL mannan. HCMV growth inhibition test indicated that at 48 h after HCMV invasion, the HCMV-DNA level in the culture supernatant with 10 μg/mL MBL was lower than the control. After 72 h, both the HCMV-DNA levels and PP65 positivity in cells incubated with MBL were reduced. This is the first to report on the anti-HCMV activities of MBL by in vitro studies.
- Published
- 2011
37. Risk factors for prolonged shedding of 2009 H1N1 influenza virus
- Author
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Shiqiang Shang, Chen Mei Zhang, Huiju Qiao, Yinghu Chen, and Meiqin Tong
- Subjects
medicine.medical_specialty ,Oseltamivir ,viruses ,Real-Time Polymerase Chain Reaction ,Antiviral Agents ,Risk Assessment ,Virus ,chemistry.chemical_compound ,Influenza A Virus, H1N1 Subtype ,Internal medicine ,Pediatric surgery ,Influenza, Human ,Medicine ,Humans ,Viral shedding ,Risk factor ,Retrospective Studies ,business.industry ,H1N1 influenza ,Infant, Newborn ,virus diseases ,Infant ,Retrospective cohort study ,H1n1 virus ,Virus Shedding ,chemistry ,Child, Preschool ,Pediatrics, Perinatology and Child Health ,Immunology ,business - Abstract
This retrospective study was conducted to estimate the shedding of 2009 H1N1 virus and the risk analysis by review of medical charts, laboratory and radiological findings of all inpatients with confirmed pandemic influenza A (H1N1) at a provincial pediatric hospital. A total of 41 cases attending the inpatient department between 15 November, 2009 to 14 December, 2009 were included. Prolonged and discontinuous shedding of 2009 H1N1 virus (median, 10 days; range, 2 to 24 days) were detected by real-time RT-PCR. The interval from onset of symptom to the start of oseltamivir therapy was an independent risk factor for prolonged virus shedding.
- Published
- 2010
38. Hemophagocytic lymphohistiocytosis at initiation of kawasaki disease and their differential diagnosis
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Tao Liu, Zihao Yang, Shiqiang Shang, Chenmei Zhang, Yongmin Tang, and Yinghu Chen
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Male ,Th2 cytokines ,Mucocutaneous Lymph Node Syndrome ,Lymphohistiocytosis, Hemophagocytic ,Diagnosis, Differential ,Th2 Cells ,medicine ,Humans ,Dexamethasone ,Hemophagocytic lymphohistiocytosis ,biology ,business.industry ,Infant ,Hematology ,Th1 Cells ,medicine.disease ,Regimen ,Oncology ,Respiratory failure ,Pediatrics, Perinatology and Child Health ,Immunology ,biology.protein ,Cytokines ,Kawasaki disease ,Differential diagnosis ,Antibody ,business ,medicine.drug - Abstract
The authors report a case of hemophagocytic lymphohistiocytosis that became apparent and was confirmed by a specific Th1/Th2 cytokine pattern at the initiation of Kawasaki disease in an 18-month-old child. His condition deteriorated fast and produced no response to intravenous immunoglobulin and dexamethasone. A standard HLH-2004 regimen was started. But he developed respiratory failure, seizure, and cardiac arrest, and died. This case is unique for developing hemophagocytic lymphohistiocytosis at the initiation of Kawasaki disease, with fatal outcome. This case may indicate a yet unknown mechanism triggers these 2 diseases; a specific Th1/Th2 cytokine pattern helps rapid differential diagnosis between these 2 diseases.
- Published
- 2010
39. CD38 gene-modified dendritic cells inhibit murine asthma development by increasing IL-12 production and promoting Th1 cell differentiation.
- Author
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JIAOLI WANG, WEIGUO ZHU, YINGHU CHEN, ZHENDONG LIN, and SHENGLIN MA
- Subjects
CYTOPROTECTION ,VASCULAR cell adhesion molecule-1 ,DENDRITIC cells ,BONE marrow ,INTERLEUKIN-12 - Abstract
Predominant T helper (Th)2 and impaired Th1 cell polarization has a crucial role in the development of asthma. Cluster of differentiation (CD)38 is associated with the increased release of interleukin (IL)-12 from dendritic cells (DCs) and DC-induced Th1 cell polarization. However, whether CD38 expression affects DC function in asthma development remains unknown. In the current study, adenoviruses were constructed containing the murine CD38 gene. Overexpression of CD38 protein level in DCs induced from bone-marrow derived DCs (BMDCs) by recombinant mouse granulocyte macrophage colony-stimulating factor and IL-4 was achieved through 24 h adenovirus infection. The results demonstrated that BMDCs with CD38 overexpression exhibited no phenotypic change; however, following stimulation with lipopolysaccharide (LPS), maturation and IL-12 secretion were increased. In addition, CD38-overexpressing BMDCs stimulated with LPS exhibited more effective Th1 cell differentiation. Mice that were administered CD38-overexpressing BMDCs exhibited milder symptoms of asthma. Furthermore, decreased IL-4, IL-5 and IL-13 levels were detected in bronchoalveolar lavage fluid (BALF), reduced immunoglobulin E levels were measured in the sera, and increased interferon-γ was detected in BALF from the recipients of CD38-overexpressing BMDCs. Increased phosphorylated-p38 expression was also detected in LPS-stimulated CD38-overexpressing BMDCs, whereas pretreatment with a p38-specific inhibitor was able to abolish the effects of LPS stimulation and CD38 overexpression on IL-12 release and Th1 cell differentiation in BMDCs. These results suggested that CD38 may be involved in the DC function of alleviating asthma via restoration of the Th1/Th2 balance, thus providing a novel strategy for asthma therapy. [ABSTRACT FROM AUTHOR]
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- 2016
- Full Text
- View/download PDF
40. Severe primary cytomegalovirus pneumonia in a 5-year-old immunocompetent child
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Yongmin Tang, Shiqiang Shang, Ru Lin, ChenMei Zhang, Tao Liu, and Yinghu Chen
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Human cytomegalovirus ,Pediatrics ,medicine.medical_specialty ,biology ,business.industry ,Respiratory disease ,Congenital cytomegalovirus infection ,medicine.disease_cause ,medicine.disease ,biology.organism_classification ,Herpesviridae ,Pneumonia ,El Niño ,Betaherpesvirinae ,Pediatrics, Perinatology and Child Health ,Immunology ,medicine ,Viral disease ,business - Published
- 2010
41. Evidence for existence of a close association between CD14 and CXCR4 on monocytic cell line U937
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Hengwen, Yang, primary, Canhui, Lan, additional, and Yinghu, Chen, additional
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- 2007
- Full Text
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42. Detection of Th1/Th2 Cytokines Is Useful for the Quick and Early Diagnosis of Hemophagocytic Syndrome in Children
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Hongqiang Shen, Chan Liao, Shi-Long Yang, Yongmin Tang, Chunfang Luo, Fen-Ying Zhao, Xiaojun Xu, Shu-Wen Shi, Binhua Pan, Baiqin Qian, Yinghu Chen, and Hua Song
- Subjects
Chemotherapy ,medicine.medical_specialty ,Univariate analysis ,business.industry ,Mortality rate ,medicine.medical_treatment ,Immunology ,Cell Biology ,Hematology ,medicine.disease ,Biochemistry ,Gastroenterology ,Sepsis ,Interleukin 10 ,Cytokine ,Internal medicine ,Medicine ,Tumor necrosis factor alpha ,Stage (cooking) ,business - Abstract
Introduction and objectives Hemophogocytic syndrome (HPS) is an infrequent disease in children with a high mortality rate. Currently, the diagnosis of HPS largely depends on non-specific clinical manifestations, but the clinical features allowing to meet the criteria of diagnosis of HPS usually do not appear simultaneously which make the early diagnosis very difficult. According to the literature, hypercytokinemia has been noticed for this disease. However, the pattern of hypercytokinemia in HPS has not been proposed. In this study, the quick determination of Th1/Th2 cytokines in the sera from patients with HPS was performed and the patterns of the cytokines were analyzed to seek for the possibility of using the cytokine pattern for the early diagnosis of HPS and its prognostic significance. Patients and Methods A total of 16 children with HPS based on the 2004 HPS criteria from June 2005 ∼ December 2006 were enrolled into this study. Peripheral blood samples were collected at the time of diagnosis, during the induction therapy and at the time of relapse. Sera from 21 healthy children, 17 ALL at remission and 17 with sepsis were used as controls. A cytometric bead assay (CBA) was used to quantitatively determine the Th1/Th2 cytokines including IL-2, IL-4, IL-6, IL-10, TNFα and IFNγ. Results Levels of IL-6 (22.20 (10.60–139.25) pg/ml), IL-10 (195.60(63.85–918.70)pg/ml) and IFNγ (4740.20(1054.72– >5000.00)pg/ml) from patients with HPS were significantly higher than those of normal control (3.35 (2.50–5.40) pg/ml, P < 0.05; 2.90(2.20–4.80)pg/ml, P5000.00)pg/ml) of IFNγ in HPS patients were much higher than those (52.80(37.50∼66.60)pg/ml, P5000.00)pg/ml, P=0.002) of prior to treatment. While those in patients insensitive to chemotherapy were not significantly reduced (P > 0.05). No significant differences in the IL-2, IL-4 and TNFα levels among patients at acute phase, remission stage and the healthy controls were observed. Univariate analysis showed that the initial IL-10 level >100pg/ml and IFNγ concentration > 4000pg/ml were both unfavorable prognostic factors for HPS (IFNγ, P =0.034; IL-10, P=0.034). Positive correlations between the levels of IFNγ and IL-10(r=0.893, P Conclusion s The quick determination of Th1/Th2 cytokines is a useful approach for the early and quick diagnosis in HPS patients based on the pattern of significant elevations of IFNγ and IL-10 with slight elevation of IL-6 and it can be used for the monitoring of the progress and the prognosis of the disease.
- Published
- 2007
43. Long-Term Follow-Up of Treatment Outcome and Prognosis in 96 Chinese Children with Acute Myeloid Leukemia: A Single Center Experience
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Baiqin Qian, Yinghu Chen, Yongmin Tang, Bo-tao Ning, Hongqiang Shen, Shi-Long Yang, Hua Song, Jian Wei, Xiaojun Xu, Shu-Wen Shi, Linyan Zhang, Wei-Qun Xu, Fen-Ying Zhao, and Binhua Pan
- Subjects
Chemotherapy ,Pediatrics ,medicine.medical_specialty ,Cumulative dose ,business.industry ,Daunorubicin ,medicine.medical_treatment ,Immunology ,Cell Biology ,Hematology ,Single Center ,Biochemistry ,Gastroenterology ,Maintenance therapy ,Internal medicine ,Homoharringtonine ,Medicine ,business ,Etoposide ,Neoadjuvant therapy ,medicine.drug - Abstract
Owing to the intensification of chemotherapy, the complete remission (CR), overall survival (OS), disease free survival (DFS) and event free survival (EFS) rates in childhood AML have been significantly improved during the past two decades in western countries. However, reports on the long-term outcome of this disease in Chinese children remain to be very limited. Objectives The aims of this study were to investigate the outcome and prognostic factors in Chinese childhood AML treated in our hospital during the past 7 years and to provide the basis for further improvement of outcome in children with AML. Methods From April 1998 to August 2005, 96 children (51 boys and 45 girls, aged 1.5 ~ 16.1 yrs with a median age of 9) with de novo AML diagnosed in our hospital based on MICM criteria were enrolled into this study. In total, 57 patients with non-APL AML were treated with the induction therapy of daunorubicin (maximum total cumulative dose 360 mg/M2) + Ara-C (DA) or homoharringtonine + Ara-C (HA), post-remission therapy with high-dose (1~2 g/M2 q12h for 6 doses) Ara-C containing regimens for three-month courses, and low-dose therapy with daunorubicin, homoharringtonine, etoposide (maximum total cumulative dose 1800 mg/M2) and Ara-C for three-month courses, followed by maintenance therapy for a total of 2~2.5 yrs. The 39 patients with APL were treated with all-trans retinoic acid (ATRA) and drugs above. Results Out of 96 patients, 86 children (89.6%) achieved CR. The estimated OS rates at three, five and seven years were (67.2±5.5)%, (58.1±6.4)% and (49.6±8.0)%, respectively, while the EFS rates were (52.3±5.8)%, (47.6±6.2)% and (47.6±6.2)%, and the DFS rates were (55.9±6.0)%, (50.7±6.4)% and (50.7±6.4)%, respectively. Univariant analysis showed that the variables predicted higher CR rates included the WBC counts (93.2% for patients < 100 × 109/L vs 50.0% for those ≥ 100 × 109/L, P = 0.004) at diagnosis, and the blasts (100% for patients without blasts vs 85.2% for those with blasts, P = 0.023) in the peripheral blood. The variables predicted longer survival duration included WBC counts ((49.1±6.5)% of 5yr EFS for patients < 100 × 109/L vs (33.3±18.0)% for those ≥ 100 × 109/L, P = 0.022) at diagnosis, FAB subtypes ((55.9±9.7)% for M3 vs (40.4±7.7)% for non-M3, P =0.048), course numbers [(76.0±12.2)% of 5-yr ESF for 1 course vs (31.1±9.0)% for more than 2 courses, P = 0.026) for achieving CR, CD38+ ((57.9± 7.3)% for CD38+ vs (16.7%± 10.8)% for CD38−, P = 0.005), CD14− ((49.9± 7.3)% for CD14− vs (21.5%± 14.2)% for CD14+, P = 0.001), CD34− ((54.7± 9.7)% for CD34− vs (32.6%± 8.8)% for CD34+, P = 0.038) and HLA-DR- ((57.7± 17.7)% for HLA-DR- vs (39.9%± 7.4)% for HLA-DR+, P = 0.024). 17 patients lost follow-up. The total cumulative relapse rate was 31.6% (25/79) with all BM relapse. The total treatment related modality rate was 5.3%. No secondary malignancy case was identified. Conclusions DA-based induction and high dose Ara-C-based post-remission treatment are effective to improve the CR rate and long-term survival in Chinese children with AML. The results are comparable to those reported in western countries. Initial WBC counts, early response to chemotherapy, FAB subtypes and some immunological markers are proved to be the significant prognostic factors for childhood AML under our current protocol.
- Published
- 2006
44. Retrospective Study on 46 Chinese Children with Acute Promyelocytic Leukemia: A Single Center Experience in China
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Hua Song, Hongqiang Shen, Shu-Wen Shi, Chan Liao, Baiqin Qian, Linyan Zhang, Yinghu Chen, Binghua Pan, Fen-Ying Zhao, Shi-Long Yang, Limin Zou, Xiaojun Xu, Bo-tao Ning, Wei-Qun Xu, Jian Wei, and Yongmin Tang
- Subjects
Acute promyelocytic leukemia ,medicine.medical_specialty ,Pediatrics ,Chemotherapy ,business.industry ,medicine.medical_treatment ,Immunology ,Cell Biology ,Hematology ,medicine.disease ,Biochemistry ,Gastroenterology ,Immunophenotyping ,Maintenance therapy ,Internal medicine ,Homoharringtonine ,medicine ,Cytarabine ,Cumulative incidence ,business ,Etoposide ,medicine.drug - Abstract
Acute promyelocytic leukemia (APL) is a specific type of hematopoietic malignancy, accounting for ~ 10% of the de novo acute myeloid leukemia (AML). During the old days, severe complications as disseminated intravascular coagulation (DIC) and intracranial hemorrhage were the most common causes of treatment failure after conventional chemotherapy without all-trans retinoic acid (RA). Owing to the application of RA for the induction treatment, the overall survival (OS), the disease free survival (DFS) and the event free survival (EFS) rates have been dramatically improved in adult patients with APL. However, data on long-term outcome of APL in children, especially in Chinese children, have been very limited. Objective The aim of this study was to investigate the clinical biological features, diagnosis, prognosis and long-term survival in childhood APL. Methods 46 children (26 boys and 20 girls, aged 1.5 ~13.8 yrs with a median of 9.3 yrs) with APL from April 1998 to October 2005 were enrolled into this study. Immunophenotyping analysis was carried out in 43 patients using multi-parameter flow cytometry. 32 patients went through PML/RARα fusion gene detection using RT-PCR. Induction treatment consisted of ATRA and daunorubicin(DNR) or pirarubicin (THP) followed by 6 courses of multi-drug chemotherapy consolidation and a long-term maintenance therapy including ATRA, high dose Ara-C (HD-Ara-C), DNR+Ara-C (DA), homoharringtonine+Ara-C (HA) and etoposide+Ara-C (EA). Results Pale, hemorrhage and fever were the most common symptoms in APL patients at the time of presentation. 19 patients (41.3%) were found to have WBC count more than 10.0×109/L at diagnosis. Immunophenotyping analysis showed that CD13, CD33, CD117 and MPO were the most commonly expressed antigens while HLA-DR, CD14 and CD34 were mostly the negative markers on APL cells. 71.9%(23/32) of the patients analyzed were PML/RARα fusion gene positive. Of the 39 patients receiving treatment, 36 children (92.3%) achieved complete remission. 7 children replased during therapy, and 3 relapsed after finishing the entire courses. The 5-year cumulative incidence of relapse (CIR) was 28.6%. The estimated overall survival (OS) rates at one, three and five years were 86.1%, 76.1%and 70.2%, respectively while the event free survival (EFS) rates were 78.4%, 63.6%and 53.1%, respectively. The probability of remaining alive after 5 years for patients with WBC≤10.0×109/L group was 81.4%, significantly higher than those with WBC>10.0×109/L group (51.6%, P=0.026). 5 children with positive PML/RARα S (short) subtype died eventually although all of them achieved CR, which was significantly different from the group with the L (long) subtype (13/13, P=0.001). Conclusion Induction with ATRA + DNR or THP is an effective and safe therapy for newly diagnosed childhood APL with very high long-term survival rates after 2.5 years of alternative multi-drug chemotherapy and maintenance. High WBC count and S subtype of PML-RARα are the two poor prognostic factors for children with APL.
- Published
- 2006
45. Risk Factors For Prolonged Shedding of 2009 H1N1 Influenza Virus.
- Author
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Yinghu Chen, Huiju Qiao, Chen Mei Zhang, Meiqin Tong, and Shiqiang Shang
- Subjects
INFLUENZA A virus, H1N1 subtype ,RISK assessment ,OSELTAMIVIR ,VIRAL load - Abstract
This retrospective study was conducted to estimate the shedding of 2009 H1N1 virus and the risk analysis by review of medical charts, laboratory and radiological findings of all inpatients with confirmed pandemic influenza A (H1N1) at a provincial pediatric hospital. A total of 41 cases attending the inpatient department between 15 November, 2009 to 14 December, 2009 were included. Prolonged and discontinuous shedding of 2009 H1N1 virus (median, 10days; range, 2 to 24 days) were detected by real-time RT-PCR. The interval from onset of symptom to the start of oseltamivir therapy was an independent risk factor for prolonged virus shedding. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
46. Enhanced Triacylglycerol Metabolism Contributes to Efficient Oil Utilization and High Production of Salinomycin in Streptomyces albus ZD11.
- Author
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Han Li, Jiaxiu Wei, Jianxin Dong, Yudong Li, Yongquan Li, Yinghu Chen, and Wenjun Guan
- Subjects
- *
SALINOMYCIN , *STREPTOMYCES , *METABOLITES , *METABOLISM , *SOY oil , *POLYKETIDES - Abstract
Streptomyces is well-known for biosynthesis of secondary metabolites with diverse bioactivities. Although oils have been employed as carbon sources to produce polyketide antibiotics for several industrial Streptomyces strains, the intrinsic correlation between oil utilization and high production of antibiotics still remains unclear. In this study, we investigate the correlation between oil metabolism and salinomycin biosynthesis in Streptomyces albus ZD11 which employs soybean oil as the main carbon source. Comparative genomic analysis revealed the enrichment of genes related to triacylglycerol (TAG) metabolism in S. albus ZD11. Transcriptomic profiling further confirmed the enhancement of TAG metabolism and acyl-coenzyme A biosynthesis in S. albus ZD11. Multiple secreted lipases, which catalyze the TAG hydrolysis, were seen to be working in a synergistic and complementary manner in aiding the efficient and stable hydrolyzation of TAGs. Together, our study suggests that enhanced TAG hydrolysis and fatty acid degradation contribute to the high-efficientcy of oil utilization in S. albus ZD11 in order to provide abundant carbon precursors for cell growth and salinomycin biosynthesis. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
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