104 results on '"Yingxin Sun"'
Search Results
2. Comparing different session regimens of electroacupuncture for chronic plantar fasciitis: Study protocol for a randomized clinical trial
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Jiaxiang Shi, Ruimin Jiao, Yan Liu, Xinkun Liu, Yingxin Sun, Hangyu Shi, Ning Gao, Zhishun Liu, Jun Liang, and Weiming Wang
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Plantar fasciitis ,Electroacupuncture ,Clinical trial ,Medicine (General) ,R5-920 - Abstract
Background: Plantar fasciitis (PF) is one of the most common causes of plantar heel pain, and previous studies found that acupuncture is effective for relieving pain in patients with PF. Nevertheless, the impact of different sessions of electroacupuncture on PF has not been investigated through randomized, controlled trials. Methods/design: This is a two parallel-group, assessor-blinded, randomized controlled trial, consisting of a four-week treatment phase followed by a 12-week follow-up. Eighty patients with chronic PF will be recruited and randomly allocated to receive 12 (three sessions per week; the multiple electroacupuncture weekly treatment group (group M)) or four (one session per week; single electroacupuncture weekly treatment group (group S)) sessions of electroacupuncture treatment in a 1:1 ratio. The primary outcome to be studied is the response rate, defined as a minimum of 50 % improvement in most severe pain intensity with first steps in the morning, compared with baseline. We will perform all analyses based on the intention-to-treat principle, with differences considered significant when the P value
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- 2024
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3. Response of Auxin, Carbohydrate and Lignin Metabolism to Habitat during the Development of Ginseng Taproot
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Meng Zhang, Yingxin Sun, Hongmei Lin, Mei Han, and Limin Yang
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Panax ginseng ,taproot expansion ,ecological factors ,physiological indices ,Agriculture - Abstract
Panax ginseng taproot serves as a crucial storage organ and constitutes a significant component of Chinese herbal medicine. In China, ginseng is cultivated using two primary methods: under-forest planting and farmland planting. These methods create distinct habitats that notably influence the morphology of ginseng taproots. However, the precise regulatory mechanisms governing ginseng taproot expansion remain to be fully elucidated. This study aimed to delineate the patterns of ginseng taproot expansion by examining taproots transplanted into farmland (TCG) and forest environments (TLCG and TQCG). Our findings indicate that light intensity and soil available potassium levels in TCG plots significantly exceed those in TLCG and TQCG plots. Compared with TLCG and TQCG, the taproot diameter of TCG increased by 11.54% and 27.73%, respectively. At the same time, combined with the microstructure of the transverse section of the taproot, it showed that the expansion of the TCG taproot was closely related to cell expansion. During TCG taproot expansion, there was an increase in indole-3-acetic acid (IAA) content, significant starch accumulation, and a decrease in lignin content. By analyzing the expression of key genes, we found that compared with TLCG and TQCG, the expression of genes PgTPS1 and PgALDH1 was upregulated, and the expression of genes PgHCT1, PgPAL3, PgPER3, and PgPER51 were downregulated in TCG taproot. Additionally, the transcription factors PgARF18.1 and PgbHLH42 were identified as responsive to habitat changes, playing pivotal roles in taproot expansion. In conclusion, this study provides foundational insights into the regulatory mechanisms of ginseng taproot expansion, offering significant implications for enhancing the quality and value of ginseng.
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- 2024
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4. Internet of things-based management versus standard management of home noninvasive ventilation in COPD patients with hypercapnic chronic respiratory failure: a multicentre randomized controlled non-inferiority trialResearch in context
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Weipeng Jiang, Xiaoyan Jin, Chunling Du, Wenchao Gu, Xiwen Gao, Chenjun Zhou, Chunlin Tu, Hongqun Chen, Hong Li, Yao Shen, Yunfeng Zhang, Xiahui Ge, Yingxin Sun, Lei Zhou, Suyun Yu, Kaishun Zhao, Qing Cheng, Xiaodan Zhu, Hongen Liao, Chunxue Bai, and Yuanlin Song
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Chronic obstructive pulmonary disease ,Noninvasive positive pressure ventilation ,Hypercapnia respiratory failure ,Internet of things ,Telemedicine ,Medicine (General) ,R5-920 - Abstract
Summary: Background: Effective monitoring and management are crucial during long-term home noninvasive positive pressure ventilation (NPPV) in patients with hypercapnic chronic obstructive pulmonary disease (COPD). This study investigated the benefit of Internet of Things (IOT)-based management of home NPPV. Methods: This multicenter, prospective, parallel-group, randomized controlled non-inferiority trial enrolled patients requiring long-term home NPPV for hypercapnic COPD. Patients were randomly assigned (1:1), via a computer-generated randomization sequence, to standard home management or IOT management based on telemonitoring of clinical and ventilator parameters over 12 months. The intervention was unblinded, but outcome assessment was blinded to management assignment. The primary outcome was the between-group comparison of the change in health-related quality of life, based on severe respiratory insufficiency questionnaire scores with a non-inferiority margin of −5. This study is registered with Chinese Clinical Trials Registry (No. ChiCTR1800019536). Findings: Overall, 148 patients (age: 72.7 ± 6.8 years; male: 85.8%; forced expiratory volume in 1 s: 0.7 ± 0.3 L; PaCO2: 66.4 ± 12.0 mmHg), recruited from 11 Chinese hospitals between January 24, 2019, and June 28, 2021, were randomly allocated to the intervention group (n = 73) or the control group (n = 75). At 12 months, the mean severe respiratory insufficiency questionnaire score was 56.5 in the intervention group and 50.0 in the control group (adjusted between-group difference: 6.26 [95% CI, 3.71–8.80]; P
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- 2024
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5. Case Report: Off-label treatment of idiopathic hypereosinophilic syndrome with Omalizumab
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Zhiyu Zhang, Yingxin Sun, and Su-Ning Chen
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omalizumab ,idiopathic hypereosinophilic syndrome ,eosinophilia ,steroid resistance ,case report ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Idiopathic hypereosinophilic syndrome (IHES) is a rare disease characterized by causeless persistent hypereosinophilia and eosinophilia-associated end-organ damage. Current treatment modalities don’t meet the needs due to adverse events of steroids as first-line therapy and the limited efficacy of second-line treatments, underscoring the need for new therapeutic strategies. Here we presented two cases of IHES with different clinical manifestations that were both refractory to corticosteroids. Patient #1 experienced rashes, cough, pneumonia, and steroid-induced side effects. Patient #2 had severe gastrointestinal symptoms attributed to hypereosinophilia. They both had high levels of serum IgE, didn’t respond well to second-line treatments of interferon-α (IFN-α) and imatinib, and Mepolizumab was not accessible. We then innovatively switched to Omalizumab, an anti-IgE monoclonal antibody approved for allergic asthma and chronic idiopathic urticaria. Patient #1 was treated with Omalizumab 600 mg per month for 20 months; his absolute eosinophil count (AEC) decreased significantly and has stabilized at around 1.0×109/L for 17 months, with complete relief from erythra and cough. Patient #2 recovered promptly from severe diarrhea with a sharp drop in AEC after 3 months of treatment with omalizumab at 600 mg per month. Therefore, we concluded that Omalizumab may be a seminal therapeutic strategy for IHES patients who are refractory to corticosteroids, whether as long-term management of AEC or as an urgent intervention to address severe symptoms caused by eosinophilia.
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- 2023
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6. Jia-Wei-Kai-Xin-San Treatment Alleviated Mild Cognitive Impairment through Anti-Inflammatory and Antiapoptotic Mechanisms in SAMP8 Mice
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Xiaolu Zhang, Yingxin Sun, Qun Yu, Wenyun Zeng, Yue Zhang, Miao Zeng, Kexin Pang, Yifei Yu, Jiali Gan, Huhu Li, Lin Yang, and Xijuan Jiang
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Pathology ,RB1-214 - Abstract
Background. Alleviating mild cognitive impairment (MCI) is crucial to delay the progression of Alzheimer’s disease (AD). Jia-Wei-Kai-Xin-San (JWKXS) is applied for treating AD with MCI. However, the mechanism of JWKXS in the treatment of MCI is unclear. Thus, this study aimed to investigate the effect and mechanism of JWKXS in SAMP8 mice models of MCI. Methods. MCI models were established to examine learning and memory ability and explore the pathomechanisms in brain of SAMP8 mice at 4, 6, and 8 months. The mice were treated for 8 weeks and the effects of JWKXS on MCI were characterized through Morris water maze and HE/Nissl’s/immunohistochemical staining. Its mechanism was predicted by the combination of UPLC-Q-TOF/MS and system pharmacology analysis, further verified with SAMP8 mice, BV2 microglial cells, and PC12 cells. Results. It was found that 4-month-old SAMP8 mice exhibited MCI. Two months of JWKXS treatment improved the learning and memory ability, alleviated the hippocampal tissue and neuron damage. Through network pharmacology, four key signaling pathways were found to be involved in treatment of MCI by JWKXS, including TLR4/NF-κB pathway, NLRP3 inflammasome activation, and intrinsic and extrinsic apoptosis. In vitro and in vivo experiments demonstrated that JWKXS attenuated neuroinflammation by inhibiting microglia activation, suppressing TLR4/NF-κB and NLRP3 inflammasome pathways, and blocking the extrinsic and intrinsic apoptotic pathways leading to neuronal apoptosis suppression in the hippocampus. Conclusion. JWKXS treatment improved the learning and memory ability and conferred neuroprotective effects against MCI by inducing anti-inflammation and antiapoptosis. Limitations. The small sample size and short duration of the intervention limit in-depth investigation of the mechanisms. Future Prospects. This provides a direction for further clarification of the anti-AD mechanism, and provides certain data support for the formulation to move toward clinical practice.
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- 2023
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7. OSR1 suppresses acute myeloid leukaemia cell proliferation by inhibiting LGR5-mediated JNK signalling
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Lingyan Zong and Yingxin Sun
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osr1 ,lgr5 ,jnk ,acute myeloid leukaemia ,cell proliferation ,Internal medicine ,RC31-1245 - Abstract
Odd-skipped related transcription factor 1 (OSR1) is implicated in various pathophysiologic processes, such as embryonic heart and urogenital formation, and functions as a tumour suppressor in diverse tumours. Regardless, the regulatory role and mechanism of OSR1 in acute myeloid leukaemia are scarce. Firstly, the CD34-positive blasts or the normal blasts were isolated from the plasma samples of acute myeloid leukaemia patients or healthy donors, respectively. Expression of OSR1 analysis by western blot and qRT-PCR showed that OSR1 was reduced in CD34-positive blasts and acute myeloid leukaemia cell lines. Secondly, acute myeloid leukaemia cell lines were transfected with pcDNA vector or shRNA for the over-expression or silence of OSR1, respectively. Functional assays demonstrated that ectopic expression of OSR1 decreased cell viability and repressed the proliferation of acute myeloid leukaemia cells, while promoted the cell apoptosis. Silence of OSR1 contributed to the proliferation of acute myeloid leukaemia cells and suppressed the cell apoptosis. Thirdly, over-expression of OSR1 decreased protein expression of leucine-rich-repeat-containing G-protein-coupled receptor 5 (LGR5) and JNK phosphorylation in the acute myeloid leukaemia cells. Ectopic expression of LGR5 attenuated OSR1 over-expression-induced decrease of LGR5 and JNK phosphorylation. Lastly, ectopic expression of LGR5 attenuated OSR1 over-expression-induced decrease of cell viability and proliferation in acute myeloid leukaemia cells. In conclusion, OSR1 functioned as a tumour suppressor in acute myeloid leukaemia cells by inhibiting LGR5-mediated activation of JNK signalling.
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- 2021
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8. The complete chloroplast genome of Clematis hexapetala (Ranunculaceae) and its phylogenetic analysis
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Yi Cui, Yingxin Sun, Yanzhe Ding, Jing Liu, Zhongming Han, Yunhe Wang, Mei Han, and Limin Yang
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clematis hexapetala ,chloroplast genome ,phylogenetic analysis ,Genetics ,QH426-470 - Abstract
Clematis hexapetala Pall. (1776) is a traditional Chinese medicine belonging to the Ranunculaceae. In this study, the complete chloroplast genome was sequenced through Illumina platform, cp was circular DNA molecule of 159,538 bp in length with a typical quadripartite structure, consisting of four regions: two copies of inverted repeat region (IRs: 31,039 bp), a large single-copy (LSC: 79,333 bp) region, a small single-copy (SSC: 18,127 bp) region. The chloroplast genome encodes a total of 135 genes, including 91 CDS genes, 36 tRNA genes, 8 rRNA genes. Phylogenetic analysis based on complete genes shows that C. hexapetala closely related to C. taeguensis in the genus Clematis. This study improves our comprehension of the chloroplast genome and its phylogenetic relationships within Ranunculaceae.
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- 2022
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9. Complete chloroplast genome characterization and phylogenetic analysis of Clematis mandshurica (Ranunculaceae)
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Yi Cui, Lihua Yang, Yanzhe Ding, Yingxin Sun, Jiao Wang, Yunfei Xi, Mei Han, Limin Yang, Zhongming Han, and Yunhe Wang
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clematis mandshurica ,chloroplast genome ,phylogenetic analysis ,Genetics ,QH426-470 - Abstract
The complete chloroplast genome sequence of Clematis mandshurica Ruprecht (1867), a specie of the Ranunculaceae family, and its phylogenetic relationships with other species have been reported in this study. The complete chloroplast genome of C. mandshurica is 159,563 bp in length, including a large single-copy (LSC) region of 79,360 bp, a small single-copy (SSC) region of 18,121 bp, and a pair of identical inverted repeat regions (IRs) of 31,041 bp. The genome encodes a total of 132 genes, including 90 protein-coding genes, 34 transfer RNA (tRNA) genes, and eight ribosomal RNA (rRNA) genes. The phylogenetic analysis reveals that C. mandshurica was found to be closest to Clematis taeguensis. The complete chloroplast genome of C. mandshurica contributes to a better understanding of phylogenetic relationships among Clematis species.
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- 2022
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10. The selective NLRP3 inhibitor MCC950 hinders atherosclerosis development by attenuating inflammation and pyroptosis in macrophages
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Wenyun Zeng, Danbin Wu, Yingxin Sun, Yanrong Suo, Qun Yu, Miao Zeng, Qing Gao, Bin Yu, Xijuan Jiang, and Yijing Wang
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Medicine ,Science - Abstract
Abstract NLRP3 inflammasome is a vital player in macrophages pyroptosis, which is a type of proinflammatory cell-death and takes part in the pathogenesis of atherosclerosis. In this study, we used apoE−/− mice and ox-LDL induced THP-1 derived macrophages to explore the mechanisms of MCC950, a selective NLRP3 inhibitor in treating atherosclerosis. For the in vivo study, MCC950 was intraperitoneal injected to 8-week-old apoE−/− mice fed with high-fat diet for 12 weeks. For the in vitro study, THP-1 derived macrophages were treated with ox-LDL and MCC950 for 48 h. MCC950 administration reduced plaque areas and macrophages contents, but did not improve the serum lipid profiles in aortic root of apoE−/− mice. MCC950 inhibited the activation of NLRP3/ASC/Caspase-1/GSDMD-N axis, and alleviated macrophages pyroptosis and the production of IL-1β and IL-18 both in aorta and in cell lysates. However, MCC950 did not affect the expression of TLR4 or the mRNA levels of NLRP3 inflammasome and its downstream proteins, suggesting that MCC950 had no effects on the priming of NLRP3 inflammasome activation in macrophages. The anti-atherosclerotic mechanisms of MCC950 on attenuating macrophages inflammation and pyroptosis involved in inhibiting the assembly and activation of NLRP3 inflammasome, rather than interrupting its priming.
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- 2021
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11. Combining Metabolomics and Transcriptomics to Reveal the Regulatory Mechanism of Taproot Enlargement in Panax ginseng
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Meng Zhang, Yingxin Sun, Ping Di, Mei Han, and Limin Yang
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garden ginseng ,taproot enlargement ,glycometabolism ,plant hormone lignin ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Ginseng is regarded as the “king of herbs” in China, with its roots and rhizomes used as medicine, and it has a high medicinal value. In order to meet the market demand, the artificial cultivation of ginseng emerged, but different growth environments significantly affect the root morphology of garden ginseng. In this study, we used ginseng cultivated in deforested land (CF-CG) and ginseng cultivated in farmland (F-CG) as experimental materials. These two phenotypes were explored at the transcriptomic and metabolomic levels so as to understand the regulatory mechanism of taproot enlargement in garden ginseng. The results show that, compared with those of F-CG, the thickness of the main roots in CF-CG was increased by 70.5%, and the fresh weight of the taproots was increased by 305.4%. Sucrose, fructose and ginsenoside were significantly accumulated in CF-CG. During the enlargement of the taproots of CF-CG, genes related to starch and sucrose metabolism were significantly up-regulated, while genes related to lignin biosynthesis were significantly down-regulated. Auxin, gibberellin and abscisic acid synergistically regulated the enlargement of the taproots of the garden ginseng. In addition, as a sugar signaling molecule, T6P might act on the auxin synthesis gene ALDH2 to promote the synthesis of auxin and, thus, participate in the growth and development of garden ginseng roots. In summary, our study is conducive to clarifying the molecular regulation mechanism of taproot enlargement in garden ginseng, and it provides new insights for the further exploration of the morphogenesis of ginseng roots.
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- 2023
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12. Pegylated Interferon Alpha-2b in Patients With Polycythemia Vera and Essential Thrombocythemia in the Real World
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Yingxin Sun, Yifeng Cai, Jiannong Cen, Mingqing Zhu, Jinlan Pan, Qian Wang, Depei Wu, and Suning Chen
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polycythemia vera ,essential thrombocythemia ,hematological response ,molecular response ,myeloproliferative neoplasms ,pegylated interferon alpha-2b ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Several clinical trials have shown promising efficacy of pegylated interferon (Peg-IFN) in the first- and second-line polycythemia vera (PV) and essential thrombocythemia (ET). However, the efficacy and safety of Peg-IFN in the real world have rarely been reported. Hence, we conducted a prospective, single-center, single-arm, open exploratory study, which aimed to explore the hematologic response, molecular response, safety, and tolerability of patients with PV and ET treated with Peg-IFN in the real world. This study included newly diagnosed or previously treated patients with PV and ET, aged 18 years or older, admitted to the Department of Hematology of the First Affiliated Hospital of Soochow University from November 2017 to October 2019. The results revealed that complete hematological response (CHR) was achieved in 66.7% of patients with PV and 76.2% of patients with ET, and the molecular response was obtained in 38.5% of patients with PV and 50% of patients with ET after 48 weeks of Peg-IFN treatment. Peg-IFN is safe, effective and well tolerated in most patients. In the entire cohort, 4 patients (9.1%) discontinued treatment due to drug-related toxicity. In conclusion, Peg-IFN is a promising strategy in myeloproliferative neoplasms (MPNs), and Peg-IFN alone or in combination with other drugs should be further explored to reduce treatment-related toxicity and improve tolerability.
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- 2021
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13. Root Physiological Changes and Transcription Analysis of Iris domestica in Response to Persistent Drought
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Qiang Ai, Yingxin Sun, Ailin Dai, Zeliang LYU, Cuijing Liu, Mei Han, and Limin Yang
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Iris domestica ,drought ,transcriptome ,root ,physiology ,Plant culture ,SB1-1110 - Abstract
Iris domestica is a popular gardening plant. Although the species is considered tolerant to drought, its growth and development are often affected by drought conditions. Therefore, revealing the regulatory mechanisms of drought tolerance in this species will aid in its cultivation and molecular breeding. In this study, morpho-physiological and transcriptome analyses of the roots of I. domestica plants were performed under persistent drought conditions. Peroxidase activity, proline content, and tectoridin content increased under sustained drought conditions. Transcriptome analysis showed that the roots of I. domestica seedlings respond to drought mainly by regulating the expression of drought-resistant genes and biosynthesis of secondary metabolites. This study provides basic data for identifying drought response mechanisms in Iris domestica.
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- 2022
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14. Diagnosis and Treatment of Myeloproliferative Neoplasms With PCM1-JAK2 Rearrangement: Case Report and Literature Review
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Yingxin Sun, Yifeng Cai, Jia Chen, Jiannong Cen, Mingqing Zhu, Jinlan Pan, Depei Wu, Aining Sun, and Suning Chen
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myeloproliferative neoplasms ,PCM1-JAK2 ,ruxolitinib ,pegylated interferon ,case report ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Myeloproliferative neoplasm (MPN) with PCM1-JAK2 rearrangement is a rare disease with poor prognosis and lacks uniform treatment guidelines. Several studies confirmed the efficacy of ruxolitinib in hematological malignancies with PCM1-JAK2 fusion, but the efficacy is variable. Here, we report two patients diagnosed with MPN with PCM1-JAK2 fusion who were treated with ruxolitinib-based regimen, including the first case of ruxolitinib combined with pegylated interferon (Peg-IFN), and we conduct a literature review. We found that ruxolitinib combined with Peg-IFN is an effective treatment option in the case of poor efficacy of ruxolitinib monotherapy.
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- 2021
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15. Identification of IGF2BP3 as an Adverse Prognostic Biomarker of Gliomas
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Chao Sun, Xin Zheng, Yingxin Sun, Ju Yu, Minfeng Sheng, Suji Yan, Qing Zhu, and Qing Lan
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glioma bioinformatics analysis ,M6A RNA methylation ,post-transcriptional modification ,prognosis ,IGF2BP3 ,Genetics ,QH426-470 - Abstract
N6-methyladenosine (m6A) RNA modification can alter gene expression and function by regulating RNA splicing, stability, translocation, and translation. It is involved in various types of cancer. However, its role in gliomas is not well known. This study aimed to determine the prognostic value of the m6A RNA methylation regulator in gliomas and investigate the underlying mechanisms of the aberrant expression of m6A-related genes.mRNA expression profiles and clinical information of 448 glioma samples were obtained from The Cancer Genome Atlas and cBioportal. The expression of m6A-related genes in normal controls and low-grade glioma and glioblastoma was obtained from Gene Expression Profiling Interactive Analysis. Further, m6A-related gene expression and its relationship with prognosis were obtained through The Chinese Glioma Genome Atlas (CGGA). Multivariate Cox regression analyses were performed, and a nomogram was built with potential risk factors based on a multivariate Cox analysis to predict survival probability. Online tools such as Gene Set Enrichment Analysis, STRING, Cytoscape, and Molecular Complex Detection were applied for bioinformatics analysis and to investigate the underlying mechanisms of the aberrant expression of m6A-related genes. The multivariate Cox regression analysis found that higher expression levels of YTHDC2 and insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3, also called IMP3) were independent negative and positive prognostic factors for overall survival (OS), respectively. Data from the CGGA database showed that IGF2BP3 expression increased when the tumor grade increased. Receiver operating characteristic (ROC) curve was used to evaluate the predictive specificity and sensitivity. The area under the ROC curve indicated that the OS prediction was 0.92 (1-year) and 0.917 (3-years), indicating that m6A-related genes could predict patient survival. In addition, IGF2BP3 was closely related to the shorter survival period of patients. Copy number variation and DNA methylation, but not somatic mutations, might contribute to the abnormal upregulation of IGF2BP3 in gliomas. Significantly altered genes were identified, and the protein–protein interaction network was constructed. Based on the data presented, our study identified several m6A-related genes, especially IGF2BP3, that could be potential prognostic biomarkers of gliomas. The study unveiled the potential regulatory mechanism of IGF2BP3 in gliomas.
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- 2021
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16. The Role of Intestinal Dysbacteriosis Induced Arachidonic Acid Metabolism Disorder in Inflammaging in Atherosclerosis
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Yingxin Sun, Danbin Wu, Wenyun Zeng, Yefei Chen, Maojuan Guo, Bin Lu, Huhu Li, Chun Sun, Lin Yang, Xijuan Jiang, and Qing Gao
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atherosclerosis ,aging ,inflammaging ,intestinal flora ,serum metabolomics ,Microbiology ,QR1-502 - Abstract
BackgroundAging induced chronic systemic inflammatory response is an important risk factor for atherosclerosis (AS) development; however, the detailed mechanism is yet to be elucidated.ObjectiveTo explore the underlying mechanism of how aging aggravates AS advancement.MethodsA young (five-week-old, YM) and aged group (32-week-old, OM) male apoE-/- mice with a high fat diet were used as models, and age-matched male wild-type C57BL/6J (WT) mice were used as controls. AS lesion size, serum lipid profile, cytokines, and gut microbiota-derived LPS were analyzed after 32 weeks of diet intervention. A correlation analysis between the 16S rRNA sequencing of the feces and serum metabolomics profiles was applied to examine the effect of their interactions on AS.ResultsApoE-/- mice developed severe atherosclerosis and inflammation in the aorta compared to the WT groups, and aged apoE-/- mice suffered from a more severe AS lesion than their younger counterparts and had low-grade systemic inflammation. Furthermore, increased levels of serum LPS, decreased levels of SCFAs production, as well as dysfunction of the ileal mucosal barrier were detected in aged mice compared with their younger counterparts. There were significant differences in the intestinal flora composition among the four groups, and harmful bacteria such as Lachnospiraceae_FCS020, Ruminococcaceae_UCG-009, Acetatifactor, Lachnoclostridium and Lactobacillus_gasseri were significantly increased in the aged apoE-/- mice compared with the other groups. Concurrently, metabolomics profiling revealed that components involved in the arachidonic acid (AA) metabolic pathway such as 20-HETE, PGF2α, arachidonic acid, and LTB4 were significantly higher in the aged AS group than in the other groups. This suggested that metabolic abnormalities and disorders of intestinal flora occurred in AS mice.ConclusionsAging not only altered the gut microbiome community but also substantially disturbed metabolic conditions. Our results confirm that AA metabolism is associated with the imbalance of the intestinal flora in the AS lesions of aged mice. These findings may offer new insights regarding the role of gut flora disorders and its consequent metabolite changed in inflammaging during AS development.
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- 2021
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17. The complete chloroplast genome of Asarum heterotropoides Fr. Schmidt var. mandshuricum (Maxim.) Kitag. (Aristolochiaceae), a traditional Chinese medicine herb
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Yingxin Sun, Qiang Ai, Zeliang Lü, and Cuijing Liu
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chloroplast genome ,aristolochiaceae ,phylogenetic analysis ,Genetics ,QH426-470 - Abstract
The complete chloroplast genome of A. heterotropoides var. mandshuricum reported herein was a circular DNA molecule of 160,262 bp in length. The typical quadripartite structure of the genome consisted of a pair of inverted repeats (IRs) of 27,262 bp separated by a large single-copy (LSC) region of 88,927 bp and a small single-copy region (SSC) of 16,811 bp. The overall GC content of the genome is 38.45%, with 36.7%, 33.1%, and 43.0% in LSC, SSC, IR regions, respectively. The cp genome encoded 125 genes, including 83 protein-coding genes, 34 tRNA genes, and 8 rRNA genes. 138 SSRs were identified in the genome. Phylogenetic anlysis showed the position of A. heterotropoides var. mandshuricum is closely related to A. heterotropoides.
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- 2021
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18. C/EBPα Suppresses Lung Adenocarcinoma Cell Invasion and Migration by Inhibiting β-Catenin
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Jinchang Lu, Chunling Du, Junxia Yao, Bo Wu, Yanhong Duan, Lei Zhou, Donghui Xu, Feng Zhou, Liang Gu, Hong Zhou, and Yingxin Sun
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C/EBPα ,Lung adenocarcinoma ,Migration ,Invasion ,β-catenin ,Physiology ,QP1-981 ,Biochemistry ,QD415-436 - Abstract
Background/Aims: The transcription factor CCAAT/enhancer-binding protein α (C/EBPα) is a basic leucine zipper transcription factor that plays essential roles in tumor progression. Although decreased or absent C/EBPα expression in many cancers suggests a possible role for C/EBPα as a tumor suppressor, the functions of C/EBPα in lung adenocarcinoma remain unclear. Methods: Here, C/EBPα expression levels in 26 lung adenocarcinoma and para-carcinoma tissue samples were detected by qRT-PCR and immunohistochemistry. Cell transwell assays, wound healing assay and three-dimensional spheroid invasion assay were performed to assess the effects of C/EBPα on migration and invasion in lung adenocarcinoma cells in vitro. Western blotting was applied to analyze the potential mechanisms. Results: C/EBPα was found to be decreased in lung adenocarcinoma tissues compared to para-carcinoma tissues. Overexpression of C/EBPα significantly inhibited the migration and invasion of lung adenocarcinoma cells. In addition, C/EBPα overexpression suppressed the epithelial–mesenchymal transition (EMT) that was characterized by a gain of epithelial and loss of mesenchymal markers. Further study showed that C/EBPα suppressed the transcription of β-catenin and downregulated the levels of its downstream targets. Conclusion: Our data suggest that C/EBPα inhibits lung adenocarcinoma cell invasion and migration by suppressing β-catenin-mediated EMT in vitro. Thus, C/EBPα may be helpful as a potential target for treatment of lung adenocarcinoma.
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- 2017
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19. The complete plastid genome of a drought-tolerant moss, Anomodon attenuatus (Hedw.) hüb
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Ziqiang Chen, Jin Yu, Linzhou Li, Yingxin Sun, Yang Peng, Qiang Ai, and Cuijing Liu
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plastid genome ,anomodon attenuatus ,phylogenetic analysis ,Genetics ,QH426-470 - Abstract
We sequenced the complete plastid genome of Anomodon attenuatus. The genome size is 125,320 bp with two inverted repeat regions of 9,948 bp separated by a large single-copy region of 86,847 bp and a small single-copy region of 18,578 bp. It encodes 114 unique genes including 80 protein-coding genes, 30 tRNA genes and 4 rRNAgenes. The overall GC content is 29.5%, with 26.9, 26.5, and 43.9% in LSC, SSC, IR regions, respectively. 139 SSRs were identified in the genome. Phylogenetic anlysis showed that the position of A.attenuatus is closely related to S.uncinata, another pleurocarpous moss.
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- 2020
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20. Motion optimization of an omnidirectional mobile robot with MY wheel based on contact mechanics.
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Changlong Ye 0001, Yingxin Sun, Suyang Yu, Jian Ding, and Chun-ying Jiang
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- 2022
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21. Multispectral Remote Sensing Data Analysis Based on KNNLC Algorithm and Multimedia Image.
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Yingxin Sun
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- 2022
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22. Phonetic interchange and interchangeable characters in a Tangut vocabulary
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Yingxin Sun
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Linguistics and Language ,History - Abstract
The famous phenomenon of ‘phonetic interchange’ in Chinese philology also occurred in Tangut scriptures. In various manuscripts and xylographs, the author or transcriber may have borrowed a phonetically related character to substitute for the original. The borrowings are not only absolute homonyms, but also show some slight distinctions in their initials or finals. In a recently discovered Tangut philological work, there is a vocabulary of over 2000 interchangeable characters enumerated in homonymic groups, which presents phonetic distinctions between Tangut oral speech and the orthodox rhyme dictionaries, and contributes to the identification of knotty characters encountered in deciphering the Tangut Buddhist texts.
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- 2023
23. Interfacial Adsorption and Corrosion Inhibition Behavior of Environmentally Friendly Imidazole Derivatives for Copper in the Marine Environment
- Author
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Li Tan, Yingxin Sun, Jiusheng Li, Sheng Han, Xiaojie Zhou, Yuzhao Tang, and Xiangqiong Zeng
- Subjects
Electrochemistry ,General Materials Science ,Surfaces and Interfaces ,Condensed Matter Physics ,Spectroscopy - Published
- 2023
24. A theoretical study on the methanol to propene mechanism catalyzed by a phosphorus-modified acidic FAU zeolite
- Author
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Mengya Xia, Qiaoxian Tong, Huai Sun, Yingxin Sun, Sheng Han, and Qianggen Li
- Subjects
Materials Chemistry ,General Chemistry ,Catalysis - Abstract
The methyl transfer pathway is the most favorable pathway in the polyMB cycle and the polyMB cycle is more competitive than the alkene cycle. The addition of the phosphorus atom in the FAU could improve the productivity of propene in the MTP process.
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- 2023
25. Ghost-RetinaNet: Fast Shadow Detection Method for Photovoltaic Panels Based on Improved RetinaNet
- Author
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Jun Wu, Penghui Fan, Yingxin Sun, and Weifeng Gui
- Subjects
Modeling and Simulation ,Software ,Computer Science Applications - Published
- 2023
26. Corrosion Detection Method of Transmission Line Components in Mining Area Based on Multiscale Enhanced Fusion
- Author
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Jun Wu, Yingxin Sun, and Xinliang Wang
- Subjects
Article Subject ,Computer Networks and Communications ,Computer Science Applications - Abstract
Component corrosion is one of the potential safety hazards in transmission lines in mining areas. In order to solve the problem of poor detection accuracy caused by the large proportion of small targets and complex background in the current distant view corrosion inspection task by UAV, we propose a PWR-YOLOV5 detection method for corrosion components based on the YOLOV5 algorithm. Firstly, a new feature fusion network, WA-PANet, is reconstructed on the basis of the path aggregation network (PANet) to make full use of the features at different stages and advance the detection accuracy of small targets in distant view by deepening the process of feature fusion and introducing the skip layer connections and adaptive feature fusion factors. Secondly, the pyramid split attention (PSA) module is introduced into the deep layers of the network to highlight the feature expression of corrosion targets and enhance the ability to detect pixel-level objects. Then, we construct a receptive feature enhancement network (RFENet), which can heighten the feature fusion effect of the WA-PANet and alleviate the problem of the feature expression ability weakening due to the fusion of different receptive field features. Finally, the EIoU Loss is adopted to optimize the loss function and improve the positioning accuracy of the bounding box. The experimental results show that the mAP of the PWR-YOLOV5 algorithm can reach up to 95.37%, which is 5.22% higher than YOLOV5, and the detection speed is 64.9FPS. Compared with the algorithms such as YOLOV4, Faster R-CNN, and YOLOX, the improved algorithm has better overall detection performance for the corrosion components of transmission lines in the mining area.
- Published
- 2022
27. Multimedia Technology of Spatial Data Mining Based on Genetic Algorithm
- Author
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Yingxin Sun
- Subjects
Article Subject ,Multimedia ,General Computer Science ,General Mathematics ,General Neuroscience ,Data Mining ,General Medicine ,Information Technology ,Algorithms - Abstract
In order to make key decisions more conveniently according to the massive data information obtained, a spatial data mining technology based on a genetic algorithm is proposed, which is combined with the k-means algorithm. The immune principle and adaptive genetic algorithm are introduced to optimize the traditional genetic algorithm, and the K-means, GK, and IGK algorithms are compared and analyzed. The results show that, in two different datasets, the objective functions obtained by the K-means algorithm are 94.05822 and 4.10373 × 10 6 , respectively, while the objective functions obtained by the GK and IGK algorithms are 89.8619 and 3.9088 × 10 6 , respectively. The difference between the three algorithms can also be reflected in the data comparison of the number of iterations. The number of iterations required for k-means to reach the optimal solution is 8.21 and 8.4, respectively, which is the most among the three algorithms, while the number of iterations required for IGK to reach the optimal solution is 5.84 and 4.9, respectively, which is the least. Although the time required for K-means is short, by comparison, the IGK algorithm we use can get the optimal solution in relatively less time.
- Published
- 2022
28. Quantum Dots and Double Surfactant-Co-modified Electromembrane Extraction of Polar Aliphatic Bioamines in Water Samples Followed by Capillary Electrophoresis with Contactless Conductivity Detection
- Author
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Qianqian Liu, Manman Wang, Jiaying Chen, Xiaofeng Jing, Yingxin Sun, Jiannong Ye, and Qingcui Chu
- Subjects
Safety, Risk, Reliability and Quality ,Safety Research ,Applied Microbiology and Biotechnology ,Food Science ,Analytical Chemistry - Published
- 2022
29. A Full-Wave FDTD-Circuit Solver For System Level ESD Modeling
- Author
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Xuezhe Tian, Yongjun Liu, Yingxin Sun, Feng Miao, and Jian Liu
- Published
- 2023
30. A theoretical investigation on the hydrodesulphurisation mechanism of hydrogenated thiophene over Cu–Mo-modified FAU zeolite
- Author
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Suyang Li, Huai Sun, Yingxin Sun, Sheng Han, and Qianggen Li
- Subjects
General Chemical Engineering ,Modeling and Simulation ,General Materials Science ,General Chemistry ,Condensed Matter Physics ,Information Systems - Abstract
We have theoretically investigated the hydrodesulphurisation (HDS) mechanism of hydrogenated thiophene over Cu–Mo-modified FAU zeolite using a two-layer ONIOM (our Own N-layered Integrated molecular Orbital and molecular Mechanics) study. The thiophene is hydrogenated to 2,3-dihydrothiophene (2,3-DHT), 2,5-dihydrothiophene (2,5-DHT) and tetrahydrothiophene (THT) due to moderate free energy barriers. Hydrogenolysis desulphurisation (HYD) and concerted direct desulphurisation (DDS) are discussed. Ring-opening, hydrogen transfer and C−S bond cleavage steps of thiophene derivatives are involved in the HYD process. The rate-determining steps are the hydrogen transfer step for 2,5-DHT and C−S bond cracking step for 2,3-DHT and THT. The concerted DDS pathway is probably more favourable than the HYD pathway in the desulphurisation of 2,5-DHT. The difference charge density (DCD) analysis reveals that for the ring-opening process, the electrons are migrated from the organic chain to the Cu–Mo catalytic centre. The reduced density gradient (RDG) plots indicate that both steric hindrance and a weak van der Waals (VDW) interaction exist between organic fragment and catalytic centre for all transition states (TSs). The localised orbital locator (LOL) maps suggest that there are strong covalent interactions and weak VDW interactions between the atoms in the forming chemical bonds.
- Published
- 2022
31. Review of Pristine Metal–Organic Frameworks for Supercapacitors: Recent Progress and Perspectives
- Author
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Zongdeng Wu, Qingli Hao, Boyuan Liu, Haiyan Jing, H.P. Wu, Yingxin Sun, Ziyu Chen, Longgang Lu, Juanjuan Song, Honglong Shen, and Haiwen Gao
- Subjects
Supercapacitor ,Fuel Technology ,Materials science ,General Chemical Engineering ,Energy Engineering and Power Technology ,Metal-organic framework ,Nanotechnology - Published
- 2021
32. BuYangHuanWu decoction alleviates atherosclerosis by regulating intestinal microbiota and metabolites in ApoE-/- mice fed with HFD
- Author
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Qun Yu, Yilin Zhang, Wenyun Zeng, Yingxin Sun, Miao Zeng, Qiuyue Yang, Lin Guo, Xiaolu Zhang, Yue Zhang, Bin Yu, Maojuan Guo, Yu Wang, Huhu Li, Yanrong Suo, Xijuan Jiang, and Lili Song
- Abstract
Background Traditional Chinese herbal prescription BuYangHuanWu decoction (BHD) works well in treating atherosclerosis. However, the mechanism of BHD in atherosclerosis has not been fully elucidated. We aimed to determine whether BHD could alleviate atherosclerosis by altering the microbiome-associated metabolic changes in atherosclerotic mice. Methods An atherosclerotic model was induced in ApoE−/− mice fed a high-fat diet (HFD), and BHD were administered by gavage for 12 weeks at 8.4g/kg/d and 16.8g/kg/d. Atherosclerotic plaque size, composition, serum lipid profile, as well as inflammatory cytokines were assessed. Mechanistically, metabolomic and micro biota profiling were analyzed using liquid chromatography mass spectrometry (LC-MS) and 16S rRNA gene sequencing, respectively. Further, intestinal microbiota and atherosclerosis-related metabolic parameters were correlated using Spearman analysis. Results Atherosclerotic mice treated with BHD showed a reduction in plaque area, aortic lumen occlusion, and lipid accumulation in the aortic root. 9 perturbed serum metabolites were significantly restored along with the relative abundance of micro biota at the family and genus levels, but not at the phylum level. Gut microbiota improvements were strongly negatively correlated with improved metabolite levels. Conclusions BHD treatment effectively slows the progression of atherosclerosis by regulating altered intestinal microbiota and perturbed metabolites. Detailed analysis of these interactions between intestinal microbes and metabolites affected by BHD will help in the development of new anti-atherosclerosis medications.
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- 2022
33. Theoretical investigation of the mechanism of ethanol to propene catalyzed by phosphorus-modified FAU zeolite
- Author
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Qiaoxian Tong, Mengya Xia, Huai Sun, Yingxin Sun, Sheng Han, and Qianggen Li
- Subjects
Physical and Theoretical Chemistry - Published
- 2022
34. A Full-Wave FDTD-Circuit Solver For Transient Analysis of Interconnects and Circuit Components
- Author
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Xuezhe Tian, Yongjun Liu, Yingxin Sun, Mingjin Zhang, and Jian Liu
- Published
- 2022
35. High Efficient Catalyst of N‐doped Carbon Modified Copper Containing Rich Cu−N−C Active Sites for Electrocatalytic CO 2 Reduction
- Author
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Yingxin Sun, Haiyan Jing, Zongdeng Wu, Jia Yu, Haiwen Gao, Yuehua Zhang, Guangyu He, Wu Lei, and Qingli Hao
- Subjects
General Chemistry - Published
- 2022
36. Clinical efficacy and quality of life effect of acetylcysteine plus pirfenidone in patients with pulmonary fibrosis
- Author
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Rong, Zhang, Zhanshuai, Song, Yan, Guan, Juan, Zhang, Jianfang, Zou, Yingxin, Sun, and Hua, Shao
- Subjects
Original Article - Abstract
Objective: To study the clinical efficacy of acetylcysteine combined with pirfenidone in patients with pulmonary fibrosis (PF). Methods: A total of 114 PF patients admitted from January 2018 to January 2019 were retrospectively analyzed. Among them, 64 patients treated with acetylcysteine combined with pirfenidone were classified into a research group, and the other 50 treated with acetylcysteine combined with budesonide were assigned into a control group. The clinical efficacy and total effectiveness rate of the two groups were compared after 6 months of therapy. The quality of life (QoL) in the two groups before and after treatment was evaluatedusing Asthma Therapy Assessment Questionnaire for idiopathic pulmonary fibrosis patients (ATAQ-IPF). The 2-year survival of the two groups was compared. Additionally, the incidence of adverse reactions was compared between the two groups. The changes in forced expiratory volume in 1 second (FEV1), forced vital capacity (FVC), inflammatory factors, and PF markers were compared between the two groups before and after therapy. Results: There were no significant differences in clinical efficacy or total effectiveness rate (all P > 0.05), serum IL-4, INFγ or IL-6 expression (all P > 0.05), as well as FEV1 and FVC levels (all P > 0.05) after therapy between two groups. After therapy, the research group showed significantly lower PCIII and HA levels, lower ATAQ-IPF scores, and lower total incidence of adverse reactions than the control group (all P < 0.05). In addition, a higher 2-year survival rate was observed in the research group than in the control group (P=0.025). Conclusion: Acetylcysteine combined with pirfenidone can reduce adverse reactions and improve the QoL and survival time of patients.
- Published
- 2022
37. Fluorescence and Physical Properties of Tm3+/Ho3+ Co-Doped Tellurite Glasses for Mid-Infrared Laser
- Author
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Yameng Wang, Manman Zhang, Yingxin Sun, Zejian Zhang, Runfa Li, Yong Wang, and Yuee Chen
- Subjects
Electronic, Optical and Magnetic Materials - Abstract
In this work, a series of Tm3+/Ho3+ co-doped tellurite glasses were successfully prepared by the melt-quenching method. The spectroscopic properties around 2 μm of Tm3+/Ho3+ co-doped tellurite glasses were studied under 808 nm laser excitation. Based on the Judd-Ofelt theory, the phenomenological intensity parameters Ω t (t = 2, 4, 6) of the Tm3+/Ho3+ co-doped tellurite glasses were calculated. In addition, the emission cross-section and the gain coefficient of Ho3+ were calculated, and the maximum values are 0.76 × 10−20 cm−1 and 1.38 cm−1, respectively. These results portend that the Tm3+/Ho3+ co-doped tellurite glass has excellent spectroscopic properties and attractive laser performance in mid-infrared waveband.
- Published
- 2023
38. Understanding the Nanoconfinement Effect on the Ethanol-to-Propene Mechanism Catalyzed by Acidic ZSM-5 and FAU Zeolites
- Author
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Sheng Han, Jiabin Yin, Qianggen Li, Xingxiang Guo, and Yingxin Sun
- Subjects
Ethanol ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Photochemistry ,01 natural sciences ,Molecular mechanics ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Catalysis ,Propene ,chemistry.chemical_compound ,General Energy ,chemistry ,Molecular orbital ,Physical and Theoretical Chemistry ,ZSM-5 ,0210 nano-technology ,Mechanism (sociology) - Abstract
We have carried out a two-layer our own n-layered integrated molecular orbital and molecular mechanics (MM) study on the mechanism of ethanol to propene on H-ZSM-5 and H-FAU zeolites. The entire me...
- Published
- 2021
39. Ghost-RetinaNet: Fast Shadow DetectionMethod for Photovoltaic Panels Based on Improved RetinaNet.
- Author
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Jun Wu, Penghui Fan, Yingxin Sun, and Weifeng Gui
- Subjects
PHOTOVOLTAIC power generation ,PHOTOVOLTAIC power systems ,MAXIMUM power point trackers ,ARTIFICIAL intelligence ,FEATURE extraction ,OBJECT recognition (Computer vision) - Abstract
Based on the artificial intelligence algorithm of RetinaNet, we propose the Ghost-RetinaNet in this paper, a fast shadow detection method for photovoltaic panels, to solve the problems of extreme target density, large overlap, high cost and poor real-time performance in photovoltaic panel shadow detection. Firstly, the Ghost CSP module based on Cross Stage Partial (CSP) is adopted in feature extraction network to improve the accuracy and detection speed. Based on extracted features, recursive feature fusion structure ismentioned to enhance the feature information of all objects. We introduce the SiLU activation function and CIoU Loss to increase the learning and generalization ability of the network and improve the positioning accuracy of the bounding box regression, respectively. Finally, in order to achieve fast detection, the Ghost strategy is chosen to lighten the size of the algorithm. The results of the experiment show that the average detection accuracy (mAP) of the algorithm can reach up to 97.17%, the model size is only 8.75 MB and the detection speed is highly up to 50.8 Frame per second (FPS), which can meet the requirements of real-time detection speed and accuracy of photovoltaic panels in the practical environment. The realization of the algorithm also provides new research methods and ideas for fault detection in the photovoltaic power generation system. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
40. Roles of Gut Microbiota in Pathogenesis of Alzheimer’s Disease and Therapeutic Effects of Chinese Medicine
- Author
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Danbin Wu, Qing Gao, Yefei Chen, Yingxin Sun, Xijuan Jiang, Bin Lu, Bin Yu, Lin Yang, Huhu Li, and Wenyun Zeng
- Subjects
Hypothalamo-Hypophyseal System ,0211 other engineering and technologies ,Pituitary-Adrenal System ,02 engineering and technology ,Traditional Chinese medicine ,Disease ,Gut flora ,Bioinformatics ,030226 pharmacology & pharmacy ,Pathogenesis ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,Alzheimer Disease ,021105 building & construction ,Humans ,Medicine ,Pharmacology (medical) ,Epigenetics ,Medicine, Chinese Traditional ,Cognitive impairment ,biology ,business.industry ,Therapeutic effect ,Brain ,Neurodegenerative Diseases ,General Medicine ,biology.organism_classification ,Gastrointestinal Microbiome ,Complementary and alternative medicine ,business - Abstract
Alzheimer's disease (AD) is the most common neurodegenerative disease characterized by progressive cognitive impairment. The pathogenesis of AD is complex, and its susceptibility and development process are affected by age, genetic and epigenetic factors. Recent studies confirmed that gut microbiota (GM) might contribute to AD through a variety of pathways including hypothalamic pituitary adrenal axis and inflflammatory and immune processes. CM formula, herbs, and monomer enjoy unique advantages to treat and prevent AD. Hence, the purpose of this review is to outline the roles of GM and its core metabolites in the pathogenesis of AD. Research progress of CMs regarding the mechanisms of how they regulate GM to improve cognitive impairment of AD is also reviewed. The authors tried to explore new therapeutic strategies to AD based on the regulation of GM using CM.
- Published
- 2020
41. Gasdermin family: a promising therapeutic target for cancers and inflammation-driven diseases
- Author
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Danbin Wu, Xijuan Jiang, Yefei Chen, Maojuan Guo, Yingxin Sun, Qing Gao, and Bin Yu
- Subjects
0301 basic medicine ,Programmed cell death ,business.industry ,Cell ,Pyroptosis ,Cancer ,Inflammation ,Review ,Cell Biology ,medicine.disease ,Biochemistry ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,Apoptosis ,030220 oncology & carcinogenesis ,medicine ,Cancer research ,medicine.symptom ,business ,Molecular Biology - Abstract
This review focuses on current advances in researches of gasdermin family. The distinctive expression patterns and biological roles of members in this family were discussed. Most of them exhibit pore-forming activity on cell membranes and are executors for programmed cell death with cytokines release, and play roles in cancers and inflammation-driven diseases. Therefore, they can be used as potential therapeutic targets to treat related diseases.
- Published
- 2020
42. Evaluation of the Role of hsa-mir-124 in Predicting Clinical Outcome in Breast Invasive Carcinoma Based on Bioinformatics Analysis
- Author
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Tongbao Feng, Jichun Tong, Xiu Han, Ping Zhang, Yingxin Sun, and Zi-Chun Hua
- Subjects
0301 basic medicine ,Oncology ,medicine.medical_specialty ,Article Subject ,Bioinformatics analysis ,Breast Neoplasms ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Biomarkers, Tumor ,medicine ,Humans ,Clinical significance ,Regulation of gene expression ,Invasive carcinoma ,General Immunology and Microbiology ,Proportional hazards model ,business.industry ,Gene Expression Profiling ,Computational Biology ,General Medicine ,Prognosis ,Phenotype ,Gene Expression Regulation, Neoplastic ,body regions ,Gene expression profiling ,MicroRNAs ,030104 developmental biology ,Lymphatic Metastasis ,030220 oncology & carcinogenesis ,embryonic structures ,Medicine ,Biomarker (medicine) ,Female ,business ,Research Article - Abstract
Purpose. Breast invasive carcinoma (BRCA) is the most common malignant tumor. MiR-124 plays a tumor-suppressive role in human cancer. However, the clinical significance of miR-124 in BRCA remains unclear. The aim of this study was to evaluate the association of hsa-mir-124 expression and the clinicopathological characteristics in BRCA using database analysis. Methods. The clinical data and expression profiles of hsa-mir-124 were obtained from the cancer genome atlas for BRCA (TCGA_BRCA). Then, the prognostic value of hsa-mir-124 in BRCA was investigated using the Cox Regression test, and the association of hsa-mir-124 and pathology TNM stages and pathologic stages were measured by the Kruskal–Wallis test and Wilcox. test. In addition, the association of hsa-mir-124 and tumor molecular phenotypes was performed using the Chi-Square test. Results. We found that the overall survival of patients with high expression of hsa-mir-124-1 and hsa-mir-124-2 was better than that of patients with low expression of hsa-mir-124-1 and hsa-mir-124-2. And the expression of hsa-mir-124-1, hsa-mir-124-2, and hsa-mir-124-3 was mainly enriched in T1/T2 stages, NO/N1 stages, and M0 stages. Then, the expression of hsa-mir-124-1, hsa-mir-124-2, and hsa-mir-124-3 was negatively associated with tumor lymph node metastasis. Moreover, the expression of hsa-mir-124 was associated with tumor molecular phenotype in breast invasive carcinoma. Conclusion. Our findings indicated that hsa-mir-124 expressions were associated with overall survival, TNM stages, pathologic characteristics, and tumor molecular phenotype in BRCA via TCGA_BRCA database, providing a new biomarker and a potential therapeutic target for BRCA patients.
- Published
- 2020
43. A comprehensive theoretical investigation on the thiophene hydrodesulphurisation mechanism over sulphided Co–Mo catalysts supported by ZSM-5, FAU, Beta and MCM-22 zeolites
- Author
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Jiabin Yin, Deping Xia, Huai Sun, Suyang Li, Yingxin Sun, Sheng Han, and Qianggen Li
- Subjects
General Chemical Engineering ,Modeling and Simulation ,General Materials Science ,General Chemistry ,Condensed Matter Physics ,Information Systems - Abstract
We have theoretically studied the hydrodesulphurisation (HDS) mechanism of thiophene and its hydrogenated derivatives over sulphided Co–Mo catalysts supported by four zeolites (ZSM-5, FAU, Beta, MCM-22) by a two-layer ONIOM (our Own N-layered Integrated molecular Orbital and molecular Mechanics) method. Due to low energy barriers, the thiophene can be hydrogenated to 2,3-dihydrothiophene (2,3-DHT), 2,5-dihydrothiophene (2,5-DHT) and tetrahydrothiophene (THT). Direct desulphurisation (DDS) and hydrogenolysis desulphurisation (HYD) pathways were investigated in detail. The calculated results show that in DDS mechanism of thiophene, the rate-determining step is the C–S bond cleavage. In HYD mechanisms of 2,5-DHT, 2,3-DHT and THT, the rate-determining step is hydrogen transfer on ZSM-5, FAU and Beta zeolites except MCM-22 zeolite. The HYD of 2,3-DHT and THT should occur by the stepwise pathway. But for the 2,5-DHT, the concerted pathway is probably more favourable than the stepwise pathway. The reduced density gradient (RDG), differential charge density (DCD), and local orbital locator (LOL) maps show that for all transition states (TSs), there are complicated van der Waals (VDW) and electrostatic interactions between the organic fragment and the catalytic centre.
- Published
- 2022
- Full Text
- View/download PDF
44. Diagnosis and Treatment of Myeloproliferative Neoplasms With PCM1-JAK2 Rearrangement: Case Report and Literature Review
- Author
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Ming-Qing Zhu, Ai-Ning Sun, Jinlan Pan, Yifeng Cai, Suning Chen, Jian-Nong Cen, De-Pei Wu, Yingxin Sun, and Jia Chen
- Subjects
Oncology ,Cancer Research ,medicine.medical_specialty ,Poor prognosis ,Ruxolitinib ,business.industry ,ruxolitinib ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,food and beverages ,medicine.disease ,myeloproliferative neoplasms ,Regimen ,Pegylated interferon ,Internal medicine ,PCM1-JAK2 ,medicine ,Effective treatment ,case report ,pegylated interferon ,business ,Myeloproliferative neoplasm ,RC254-282 ,Rare disease ,medicine.drug - Abstract
Myeloproliferative neoplasm (MPN) with PCM1-JAK2 rearrangement is a rare disease with poor prognosis and lacks uniform treatment guidelines. Several studies confirmed the efficacy of ruxolitinib in hematological malignancies with PCM1-JAK2 fusion, but the efficacy is variable. Here, we report two patients diagnosed with MPN with PCM1-JAK2 fusion who were treated with ruxolitinib-based regimen, including the first case of ruxolitinib combined with pegylated interferon (Peg-IFN), and we conduct a literature review. We found that ruxolitinib combined with Peg-IFN is an effective treatment option in the case of poor efficacy of ruxolitinib monotherapy.
- Published
- 2021
45. The selective NLRP3 inhibitor MCC950 hinders atherosclerosis development by attenuating inflammation and pyroptosis in macrophages
- Author
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Yijing Wang, Miao Zeng, Qing Gao, Xijuan Jiang, Qun Yu, Yingxin Sun, Danbin Wu, Yanrong Suo, Bin Yu, and Wenyun Zeng
- Subjects
Male ,Mice, Knockout, ApoE ,Science ,Priming (immunology) ,Inflammation ,Article ,Proinflammatory cytokine ,Pathogenesis ,Mice ,In vivo ,NLR Family, Pyrin Domain-Containing 3 Protein ,medicine ,Pyroptosis ,Animals ,Humans ,Furans ,Pharmacology ,Sulfonamides ,Multidisciplinary ,integumentary system ,Chemistry ,Macrophages ,Small molecules ,Inflammasome ,Atherosclerosis ,Cardiovascular biology ,Cell biology ,Disease Models, Animal ,Indenes ,TLR4 ,Medicine ,medicine.symptom ,medicine.drug - Abstract
NLRP3 inflammasome is a vital player in macrophages pyroptosis, which is a type of proinflammatory cell-death and takes part in the pathogenesis of atherosclerosis. In this study, we used apoE−/− mice and ox-LDL induced THP-1 derived macrophages to explore the mechanisms of MCC950, a selective NLRP3 inhibitor in treating atherosclerosis. For the in vivo study, MCC950 was intraperitoneal injected to 8-week-old apoE−/− mice fed with high-fat diet for 12 weeks. For the in vitro study, THP-1 derived macrophages were treated with ox-LDL and MCC950 for 48 h. MCC950 administration reduced plaque areas and macrophages contents, but did not improve the serum lipid profiles in aortic root of apoE−/− mice. MCC950 inhibited the activation of NLRP3/ASC/Caspase-1/GSDMD-N axis, and alleviated macrophages pyroptosis and the production of IL-1β and IL-18 both in aorta and in cell lysates. However, MCC950 did not affect the expression of TLR4 or the mRNA levels of NLRP3 inflammasome and its downstream proteins, suggesting that MCC950 had no effects on the priming of NLRP3 inflammasome activation in macrophages. The anti-atherosclerotic mechanisms of MCC950 on attenuating macrophages inflammation and pyroptosis involved in inhibiting the assembly and activation of NLRP3 inflammasome, rather than interrupting its priming.
- Published
- 2021
46. A Theoretical Investigation on the Hydrodesulfurization Mechanism of Hydrogenated Thiophene Over Cu-mo Modified Fau Zeolite
- Author
-
Suyang Li, Yingxin Sun, Sheng Han, and Qianggen Li
- Subjects
chemistry.chemical_compound ,Materials science ,Chemical engineering ,chemistry ,Thiophene ,Zeolite ,Hydrodesulfurization ,Mechanism (sociology) - Abstract
We have carried out a two-layer our own n-layered integrated molecular orbital and molecular mechanics (ONIOM) study on the hydrodesulfurization (HDS) mechanism of three hydrogenated thiophene derivatives over Cu-Mo modified FAU zeolite. The thiophene is hydrogenated relatively easily to 2,3-dihydrothiophene (2,3-DHT), 2,5-dihydrothiophene (2,5-DHT) and tetrahydrothiophene (THT) due to low free energy barriers. Hydrogenolysis desulfurization (HYD) and direct desulfurization (DDS) are discussed. Ring-opening, hydrogen transfer and C−S bond cleavage steps of thiophene derivatives are involved in the HYD process. The rate-determining steps are the hydrogen transfer step for 2,5-DHT and C−S bond cracking step for 2,3-DHT and THT. The concerted DDS pathway is probably more favorable than the HYD pathway in the desulfurization of 2,5-DHT. The hydrogenation of thiophene to 2,5-DHT, 2,3-DHT and THT and their HDS process are entropy-decreased; the formation of sulfur vacancy is entropy-increased. The difference charge density (DCD) analysis reveals that for the ring-opening process, the electrons are migrated from the organic chain to the Cu-Mo catalytic center. The reduced density gradient (RDG) plots indicate that both a steric hindrance and a weak van der Waals attractive interaction exist between organic fragment and catalytic center for all transition states (TSs). The localized orbital locator (LOL) maps for all TSs suggest that there are strong covalent interactions between the atoms in the forming chemical bonds and weak van der Waals interactions between the atoms in the breaking chemical bonds.
- Published
- 2021
47. Identification of IGF2BP3 as an Adverse Prognostic Biomarker of Gliomas
- Author
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Minfeng Sheng, Qing Zhu, Xin Zheng, Qing Lan, Yingxin Sun, Suji Yan, Chao Sun, and Ju Yu
- Subjects
IGF2BP3 ,RNA methylation ,Cancer ,QH426-470 ,Biology ,medicine.disease ,Gene expression profiling ,Glioma ,Gene expression ,DNA methylation ,medicine ,Cancer research ,Genetics ,Molecular Medicine ,Copy-number variation ,prognosis ,glioma bioinformatics analysis ,Gene ,M6A RNA methylation ,Genetics (clinical) ,post-transcriptional modification ,Original Research - Abstract
N6-methyladenosine (m6A) RNA modification can alter gene expression and function by regulating RNA splicing, stability, translocation, and translation. It is involved in various types of cancer. However, its role in gliomas is not well known. This study aimed to determine the prognostic value of the m6A RNA methylation regulator in gliomas and investigate the underlying mechanisms of the aberrant expression of m6A-related genes.mRNA expression profiles and clinical information of 448 glioma samples were obtained from The Cancer Genome Atlas and cBioportal. The expression of m6A-related genes in normal controls and low-grade glioma and glioblastoma was obtained from Gene Expression Profiling Interactive Analysis. Further, m6A-related gene expression and its relationship with prognosis were obtained through The Chinese Glioma Genome Atlas (CGGA). Multivariate Cox regression analyses were performed, and a nomogram was built with potential risk factors based on a multivariate Cox analysis to predict survival probability. Online tools such as Gene Set Enrichment Analysis, STRING, Cytoscape, and Molecular Complex Detection were applied for bioinformatics analysis and to investigate the underlying mechanisms of the aberrant expression of m6A-related genes. The multivariate Cox regression analysis found that higher expression levels of YTHDC2 and insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3, also called IMP3) were independent negative and positive prognostic factors for overall survival (OS), respectively. Data from the CGGA database showed that IGF2BP3 expression increased when the tumor grade increased. Receiver operating characteristic (ROC) curve was used to evaluate the predictive specificity and sensitivity. The area under the ROC curve indicated that the OS prediction was 0.92 (1-year) and 0.917 (3-years), indicating that m6A-related genes could predict patient survival. In addition, IGF2BP3 was closely related to the shorter survival period of patients. Copy number variation and DNA methylation, but not somatic mutations, might contribute to the abnormal upregulation of IGF2BP3 in gliomas. Significantly altered genes were identified, and the protein–protein interaction network was constructed. Based on the data presented, our study identified several m6A-related genes, especially IGF2BP3, that could be potential prognostic biomarkers of gliomas. The study unveiled the potential regulatory mechanism of IGF2BP3 in gliomas.
- Published
- 2021
48. Target of MCC950 in Inhibition of NLRP3 Inflammasome Activation: a Literature Review
- Author
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Danbin Wu, Yangxue Wang, Qing Gao, Huhu Li, Yingxin Sun, Yefei Chen, Zhengfei Yang, Bin Yu, and Xijuan Jiang
- Subjects
0301 basic medicine ,Inflammasomes ,Potassium ,Inflammatory response ,Immunology ,Anti-Inflammatory Agents ,chemistry.chemical_element ,Heterocyclic Compounds, 4 or More Rings ,Chloride ,03 medical and health sciences ,0302 clinical medicine ,NLR Family, Pyrin Domain-Containing 3 Protein ,medicine ,Animals ,Humans ,Immunology and Allergy ,Molecular Targeted Therapy ,Sulfones ,Furans ,Inflammation ,Sulfonamides ,Small molecule ,Cell biology ,030104 developmental biology ,Indenes ,chemistry ,030220 oncology & carcinogenesis ,Efflux ,NLRP3 inflammasome activation ,Function (biology) ,Intracellular ,Signal Transduction ,medicine.drug - Abstract
MCC950 has been proposed as a specific small molecule inhibitor that can selectively block NLRP3 inflammasome activation. However, the exact mechanism of its action is still ambiguous. Accumulating investigations imply that chloride efflux-dependent ASC speck oligomerization and potassium efflux-dependent activation of caspase-1 are the two relatively independent, but indispensable events for NLRP3 inflammasome activation. Previous studies suggested that influence of MCC950 on potassium efflux and its consequent events such as interaction between NEK7 and NLRP3 are limited. However, inhibiting chloride intracellular channel-dependent chloride efflux leads to a modification of inflammatory response, which is similar to the function of MCC950. Based on these findings, we shed new insights on the understanding of MCC950 that its function might correlate with chloride efflux, chloride intracellular channels, or other targets that act upstream of chloride efflux.
- Published
- 2019
49. Theoretical studies on Rh(<scp>iii</scp>)-catalyzed regioselective C–H bond cyanation of indole and indoline
- Author
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Weihua Zhang, Yingxin Sun, Chao Deng, and Yi Ren
- Subjects
Indole test ,Reaction mechanism ,010405 organic chemistry ,Chemistry ,Regioselectivity ,Cyanation ,010402 general chemistry ,01 natural sciences ,Medicinal chemistry ,0104 chemical sciences ,Catalysis ,Inorganic Chemistry ,chemistry.chemical_compound ,Nucleophile ,Indoline ,Density functional theory - Abstract
Density functional theory calculations were carried out to study the reaction mechanism of the Rh(iii)-catalyzed regioselective C-H cyanation of indole and indoline with N-cyano-N-phenyl-para-methylbenzenesulfonamide (NCTS). This mechanism involves four major steps: C-H activation, cyano group insertion, β-N elimination, and regeneration of active species. How different indole and indoline substrates affect the regioselectivity of C-H bond cyanation has been examined and analyzed in detail. Our calculation results indicate that the regioselectivity of C-H bond cyanation of indole depends on the nucleophilicity of carbon atoms in C-Rh(iii) bonds to the cyano group. For indoline, it can be attributed to the different hybridization platforms of the C-H bond activation.
- Published
- 2019
50. Copper-mediated cascade radical cyclization of olefins with naphthalenyl iododifluoromethyl ketones
- Author
-
Jingjing Wu, Peng Peng, Yingxin Sun, Guo Zhi Huang, Wang Xing, and Fanhong Wu
- Subjects
010405 organic chemistry ,Drug discovery ,Chemistry ,Organic Chemistry ,Copper mediated ,Regioselectivity ,chemistry.chemical_element ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Combinatorial chemistry ,Radical cyclization ,Copper ,0104 chemical sciences ,Cascade ,Density functional theory ,Physical and Theoretical Chemistry - Abstract
Copper-mediated radical cyclization of naphthalenyl iododifluoromethyl ketones with olefins was successfully developed to generate a series of unprecedented gem-difluorodihydrophenanthrenones, especially 2,2-difluoro-3,4-dihydrophenanthren-1(2H)-one derivatives. This strategy features the use of cheap copper powder and excellent regioselectivity and diastereoselectivity, thus providing a facile approach for application in drug discovery and development. Preliminary mechanistic studies indicate the involvement of difluorinated radical intermediates. Density functional theory (DFT) calculation was performed to provide further evidence for regioselectivity.
- Published
- 2019
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