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In this paper we report on a survey study of teachers’ perceptions of how mathematics textbooks, including broadly student books, teacher manuals, and exercise books, facilitate or hinder teachers’ teaching in Shanghai educational settings. For the study we established a conceptual framework about teachers’ teaching, partly drawing on Shulman’s model of pedagogical reasoning and action of teachers. The data were collected from a stratified random sample of 133 mathematics teachers in 13 secondary schools through a questionnaire, and follow-up interviews with 24 of them. The results revealed the following: the textbooks were highly regarded and used by the Shanghai mathematics teachers as facilitators rather than barriers for their teaching and instructional change, and the facilitation was most evident in the process of transformation and comprehension, and least evident in evaluation in teachers’ pedagogical reasoning and action; compared with student books and exercise books, teacher manuals played a larger facilitating role in teachers’ teaching; and finally, compared with teacher characteristics, school characteristics had a greater influence on the extent to which textbooks played a role of facilitation or hindrance for teachers’ instructional practice. Implications and suggestions are given at the end of the paper. Supplementary Information The online version contains supplementary material available at 10.1007/s11858-021-01306-6.

By geometrically constraining fluids into the sub-millimeter scale, microfluidics offers a physical environment largely different from the macroscopic world, as a result of the significantly enhanced surface effects. This environment is characterized by laminar flow and inertial particle behavior, short diffusion distance, and largely enhanced heat exchange. The recent two decades have witnessed the rapid advances of microfluidic technologies in various fields such as biotechnology; analytical science; and diagnostics; as well as physical, chemical, and biological research. On the other hand, one additional field is still emerging. With the advances in nanomaterial and soft matter research, there have been some reports of the advantages discovered during attempts to synthesize these materials on microfluidic chips. As the formation of nanomaterials and soft matters is sensitive to the environment where the building blocks are fed, the unique physical environment of microfluidics and the effectiveness in coupling with other force fields open up a lot of possibilities to form new products as compared to conventional bulk synthesis. This Perspective summarizes the recent progress in producing novel functional materials using microfluidics, such as generating particles with narrow and controlled size distribution, structured hybrid materials, and particles with new structures, completing reactions with a quicker rate and new reaction routes and enabling more effective and efficient control on reactions. Finally, the trend of future development in this field is also discussed.

The optical response of metal nanoparticles is governed by plasmonic resonances, which are dictated by the particle morphology. A thorough understanding of the link between morphology and optical response requires quantitatively measuring optical and structural properties of the same particle. Here we present such a study, correlating electron tomography and optical micro-spectroscopy. The optical measurements determine the scattering and absorption cross-section spectra in absolute units, and electron tomography determines the 3D morphology. Numerical simulations of the spectra for the individual particle geometry, and the specific optical set-up used, allow for a quantitative comparison including the cross-section magnitude. Silver nanoparticles produced by photochemically driven colloidal synthesis, including decahedra, tetrahedra and bi-tetrahedra are investigated. A mismatch of measured and simulated spectra is found in some cases when assuming pure silver particles, which is explained by the presence of a few atomic layers of tarnish on the surface, not evident in electron tomography. The presented method tightens the link between particle morphology and optical response, supporting the predictive design of plasmonic nanomaterials.

We demonstrate 1.8 W of average output power with 350 fs of pulse duration from a diode-pumped mode-locked rotary Nd:glass disk laser. In this laser with rotating Nd:glass disk structure, we use a Nd:glass disk with a diameter of 50 mm, which disperses heat to a wide area, to extend the upper limit of pumping power of Nd:glass for high output average power. We achieve passively soliton mode-locking for obtaining femtosecond pulses based on a semiconductor saturable absorber mirror. To our best knowledge, this is the highest average power reported from a diode-pumped femtosecond Nd:glass laser oscillator. The results are significant for power scaling of femtosecond Nd:glass lasers.

The optical response of metal nanoparticles is governed by plasmonic resonances, which depend often intricately on the geometry and composition of the particle and its environment. In this work we describe a method and analysis pipeline unravelling these relations at the single nanoparticle level through a quantitative characterization of the optical and structural properties. It is based on correlating electron microscopy with microspectroscopy measurements of the same particle immersed in media of different refractive indices. The optical measurements quantify the magnitude of both the scattering and the absorption cross sections, while the geometry measured in electron microscopy is used for numerical simulations of the cross section spectra accounting for the experimental conditions. We showcase the method on silver nanocubes of nominal 75 nm edge size. The large amount of information afforded by the quantitative cross section spectra and measuring the same particle in two environments, allows us to identify a specific degradation of the cube surface. We find a layer of tarnish, only a few nanometers thick, a fine surface compositional change of the studied system which would be hardly quantifiable otherwise.

At fertilization in mice and humans, the activation of the egg is caused by a series of repetitive Ca2+ oscillations which are initiated by phospholipase-C(zeta)ζ that generates inositol-1,4,5-trisphophate (InsP3). Ca2+ oscillations and egg activation can be triggered in mature mouse eggs by incubation in Sr2+ containing medium, but this does not appear to be effective in human eggs. Here, we have investigated the reason for this apparent difference using mouse eggs, and human eggs that failed to fertilize after IVF or ICSI. Mouse eggs incubated in Ca2+-free, Sr2+-containing medium immediately underwent Ca2+ oscillations but human eggs consistently failed to undergo Ca2+ oscillations in the same Sr2+ medium. We tested the InsP3-receptor (IP3R) sensitivity directly by photo-release of caged InsP3 and found that mouse eggs were about 10 times more sensitive to InsP3 than human eggs. There were no major differences in the Ca2+ store content between mouse and human eggs. However, we found that the ATP concentration was consistently higher in mouse compared to human eggs. When ATP levels were lowered in mouse eggs by incubation in pyruvate-free medium, Sr2+ failed to cause Ca2+ oscillations. When pyruvate was added back to these eggs, the ATP levels increased and Ca2+ oscillations were induced. This suggests that ATP modulates the ability of Sr2+ to stimulate IP3R-induced Ca2+ release in eggs. We suggest that human eggs may be unresponsive to Sr2+ medium because they have a lower level of cytosolic ATP.

Many rapid antimicrobial susceptibility testing (AST) methods have been proposed to contain clinical antimicrobial resistance (AMR) and preserve the effectiveness of remaining antimicrobials. However, far fewer methods have been proposed to test AMR in resource-limited conditions, such as for frequent safety screenings of water/food/public facilities, urgent surveys of massive samples during a pandemic, or AMR tests in low-income countries. Rapid AST methods realized thus far have a variety of drawbacks when used for such surveys, e.g., high cost and the requirement of expensive instruments such as microscopy. A more reasonable strategy would be to screen samples via onsite testing first, and then send any sample suspected to contain AMR bacteria for advanced testing. Accordingly, a cost-efficient AST is demanded, which can rapidly process a large number of samples without using expensive equipment. To this end, current work demonstrates a novel “barcode” cell sensor based on an adaptive linear filter array as a fully automatic and microscope-free method for counting very small volumes of cells (~1.00 × 104 cells without pre-incubation), wherein suspended cells concentrate into microbars with length proportional to the number of cells. We combined this sensor with an on-chip culture approach we had demonstrated for rapid and automated drug exposure and realized a low-cost and resource-independent platform for portable AST, from which results can be obtained simply through a cell phone. This method has a much shorter turnaround time (2–3 h) than that of standard methods (16–24 h). Thanks to its microscopy-free analysis, affordability, portability, high throughput, and user-friendliness, our “barcode” AST system has the potential to fulfill the various demands of AST when advanced facilities are not available, making it a promising new tool in the fight against AMR.

Defect engineering of enzyme-MOF composites by microfluidics engenders high enzymatic activity., Mimicking the cellular environment, metal-organic frameworks (MOFs) are promising for encapsulating enzymes for general applications in environments often unfavorable for native enzymes. Markedly different from previous researches based on bulk solution synthesis, here, we report the synthesis of enzyme-embedded MOFs in a microfluidic laminar flow. The continuously changed concentrations of MOF precursors in the gradient mixing on-chip resulted in structural defects in products. This defect-generating phenomenon enables multimodal pore size distribution in MOFs and therefore allows improved access of substrates to encapsulated enzymes while maintaining the protection to the enzymes. Thus, the as-produced enzyme-MOF composites showed much higher (~one order of magnitude) biological activity than those from conventional bulk solution synthesis. This work suggests that while microfluidic flow synthesis is currently underexplored, it is a promising strategy in producing highly active enzyme-MOF composites.

Using an antimicrobial susceptibility test (AST) as an example, this work demonstrates a practical method to fabricate microfluidic chips entirely from polypropylene (PP) and the benefits for potential commercial use. Primarily caused by the misuse and abuse of antibiotics, antimicrobial resistance (AMR) is a major threat to modern medicine. The AST is a promising technique to help with the optimal use of antibiotics for reducing AMR. However, current phenotypic ASTs suffer from long turnaround time, while genotypic ASTs suffer from low reliability, and both are unaffordable for routine use. New microfluidics based AST methods are rapid but still unreliable as well as costly due to the PDMS chip material. Herein, we demonstrate a convenient method to fabricate whole PP microfluidic chips with high resolution and fidelity. Unlike PDMS chips, the whole PP chips showed better reliability due to their inertness; they are solvent-compatible and can be conveniently reused and recycled, which largely decreases the cost, and are environmentally friendly. We specially designed 3D chambers that allow for quick cell loading without valving/liquid exchange; this new hydrodynamic design satisfies the shear stress requirement for on-chip bacterial culture, which, compared to reported designs for similar purposes, allows for a simpler, more rapid, and high-throughput operation. Our system allows for reliable tracking of individual cells and acquisition of AST results within 1–3 hours, which is among the group of fastest phenotypic methods. The PP chips are more reliable and affordable than PDMS chips, providing a practical solution to improve current culture-based AST and benefiting the fight against AMR through helping doctors prescribe effective, narrow-spectrum antibiotics; they will also be broadly useful for other applications wherein a reliable, solvent-resistant, anti-fouling, and affordable microfluidic chip is needed.

Mammalian eggs generate most of their ATP by mitochondrial oxidation of pyruvate from the surrounding medium or from fatty acids that are stored as triacylglycerols within lipid droplets. The balance between pyruvate and fatty acid oxidation in generating ATP is not established. We have combined coherent anti-Stokes Raman scattering (CARS) imaging with deuterium labelling of oleic acid to monitor turnover of fatty acids within lipid droplets of living mouse eggs. We found that loss of labelled oleic acid is promoted by pyruvate removal but minimised when β-oxidation is inhibited. Pyruvate removal also causes a significant dispersion of lipid droplets, while inhibition of β-oxidation causes droplet clustering. Live imaging of luciferase or FAD autofluorescence from mitochondria, suggest that inhibition of β-oxidation in mouse eggs only leads to a transient decrease in ATP because there is compensatory uptake of pyruvate into mitochondria. Inhibition of pyruvate uptake followed by β-oxidation caused a similar and successive decline in ATP. Our data suggest that β-oxidation and pyruvate oxidation contribute almost equally to resting ATP production in resting mouse eggs and that reorganisation of lipid droplets occurs in response to metabolic demand.

We present a wide-field imaging technique recently developed by us to measure quantitatively the optical extinction cross section σext of individual nanoparticles. The technique is simple, high speed, and enables the simultaneous acquisition of hundreds of nanoparticles in the wide-field image for statistical analysis, with a sensitivity corresponding to the detection of a single gold nanoparticle down to 2nm diameter. Notably, the method is applicable to any nanoparticle (dielectric, semiconducting, metallic), and can be easily and cost-effectively implemented on a conventional wide-field microscope. Of specific significance for accurate quantification, we show that σext depends on the numerical aperture of the microscope illumination due to the oblique incidence, even for spherical particles in an isotropic environment. This "long shadow" effect needs to be taken into account when comparing σext to theoretical values calculated under plane wave illumination at normal incidence. Owing to the accurate experimental quantification of σext, one can then use it to determine the nanoparticle size, as demonstrated here on gold nanoparticles of 30nm nominal diameter. This technique thus has the potential to become a simple and cost-effective new tool for accurate size characterization of single small nanoparticles, complementing time consuming and expensive methods such as electron microscopy.

During the past two decades, microfluidics has become an enabling technology in many fields, including cell-biology, biophysics, biochemistry, optofluidics, etc. In the meantime, researchers are paying more attention to device materials because of the problems and unique functions they brought. As the most popular material in current microfluidic research, polydimethylsiloxane (PDMS) also has some drawbacks, such as absorption of small molecules and poor organic solvent compatibility. Thus, perfluorinated polymers (Teflons), which retain most advantages of PDMS but also has excellent inertness and anti-fouling properties, came into researchers’ perspective. After the initial period when liquid Teflon were directly coated on PDMS, whole-Teflon microfluidic chips were successfully fabricated and performed excellently in long-term cell culture, organic solvent-involved synthesis, etc. Since then, materials such as metals, biomolecules and other plastics have been integrated into Teflon chips and expanded their use to electrochemical sensing and immunoassay. Furthermore, perfluoropolymers have also been broadly used to assist mold release due to their nonstick property. Aiming to illustrate this progress, current review focuses on the recent development of Teflon microfabrication and applications in microfluidics, and provides critical discussion on this trend.

Crosstalk between the microtubule (MT) and actin cytoskeletons is fundamental to many cellular processes including cell polarisation and cell motility. Previous work has shown that members of the growth-arrest-specific 2 (GAS2) family mediate the crosstalk between filamentous actin (F-actin) and MTs, but the molecular basis of this process remained unclear. By using fluorescence microscopy, we demonstrate that three members of this family, GAS2-like 1, GAS2-like 2 and GAS2-like 3 (G2L1, G2L2 and G2L3, also known as GAS2L1, GAS2L2 and GAS2L3, respectively) are differentially involved in mediating the crosstalk between F-actin and MTs. Although all localise to actin and MTs, only the exogenous expression of G2L1 and G2L2 influenced MT stability, dynamics and guidance along actin stress fibres. Biochemical analysis and live-cell imaging revealed that their functions are largely due to the association of these proteins with MT plus-end-binding proteins that bind to SxIP or SxLP motifs located at G2L C-termini. Our findings lead to a model in which end-binding (EB) proteins play a key role in mediating actin–MT crosstalk.

In the recent few years, the UFH RFID systems which work in the frequency range between 860MHz and 960MHz have been widely adopted in real deployments and applications, and accordingly the EPC Gen-2 protocol is proposed as an international standard to resolve the tag collisions occurred in such system. In this paper, based on the stochastic distribution model established to analyze the collision resolution process of this protocol, some key factors that affect its performance are discussed, and a transmission control schema based on the stochastic model is proposed to optimize this protocol. Simulations are performed to examine this optimization and the its performance is verified regarding to the average number of queries to collect a tag identifier.