1. Downregulation of miR‑193a‑3p via targeting cyclin D1 in thyroid cancer
- Author
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Li‑Jie Zhang, Rong Wen, Hong Yang, Yong‑Ying Qin, Jun‑Lin Yang, Jun Wang, Deng‑Hua Pan, Yu‑Yan Pang, Qin‑Qiao Lai, Yu He, Jing‑Ni Lai, Xiao‑Jiao Li, Lin Shi, Jun Ma, Dong‑Yue Wen, Yun‑Hui Lai, and Peng Lin
- Subjects
Male ,0301 basic medicine ,Cancer Research ,Microarray ,cyclin D1 ,Biology ,Biochemistry ,03 medical and health sciences ,0302 clinical medicine ,Cyclin D1 ,microRNA ,thyroid cancer ,Genetics ,medicine ,Humans ,Thyroid Neoplasms ,KEGG ,Molecular Biology ,Gene ,Neoplasm Staging ,Oligonucleotide Array Sequence Analysis ,Regulation of gene expression ,Sequence Analysis, RNA ,Gene Expression Profiling ,in-house dual luciferase assay ,Cancer ,Articles ,Prognosis ,medicine.disease ,Survival Analysis ,microRNA-193a-3p ,Molecular medicine ,Gene Expression Regulation, Neoplastic ,meta-analysis ,MicroRNAs ,030104 developmental biology ,Oncology ,030220 oncology & carcinogenesis ,Cancer research ,Molecular Medicine ,Female - Abstract
Thyroid cancer (TC) is a frequently occurring malignant tumor with a rising steadily incidence. microRNA (miRNA/miR)-193a-3p is an miRNA that is associated with tumors, playing a crucial role in the genesis and progression of various cancers. However, the expression levels of miR-193a-3p and its molecular mechanisms in TC remain to be elucidated. The present study aimed to probe the expression of miR-193a-3p and its clinical significance in TC, including its underlying molecular mechanisms. Microarray and RNA sequencing data gathered from three major databases, specifically Gene Expression Omnibus (GEO), ArrayExpress and The Cancer Genome Atlas (TCGA) databases, and the relevant data from the literature were used to examine miR-193a-3p expression. Meta-analysis was also conducted to evaluate the association between clinicopathological parameters and miR-193a-3p in 510 TC and 59 normal samples from the TCGA database. miRWalk 3.0, and the TCGA and GEO databases were used to predict the candidate target genes of miR-193a-3p. Gene Ontology, Kyoto Encyclopedia of Genes and Genomes and protein-protein interaction network enrichment analyses were conducted by using the predicted candidate target genes to investigate the underlying carcinogenic mechanisms. A dual luciferase assay was performed to validate the targeting regulatory association between the most important hub gene cyclin D1 (CCND1) and miR-193a-3p. miR-193a-3p expression was considerably downregulated in TC compared with in the non-cancer controls (P
- Published
- 2020