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8 results on '"Yong-ling Qin"'

1. Production of Polyunsaturated Fatty Acids by Mucor recurvus sp. with Sugarcane Molasses as the Carbon Source

Author

Zhi-Nian Deng, Nan Li, Yong-Ling Qin, Chun-Lan Chen, and Zhi-Qun Liang

Subjects
Mucor recurvus sp., polyunsaturated fatty acids, sugarcane molasses, fermentation, cultivation condition, fatty acid analysis, Biotechnology, TP248.13-248.65, Food processing and manufacture, TP368-456
Abstract

Sugarcane molasses is rich in nutriments and vitamins. It may be used as the carbon source for the production of polyunsaturated fatty acids (PUFA) by Mucor recurvus sp. Using sugarcane molasses, the effects of fermentation parameters and media components on polyunsaturated fatty acid production through both linear and orthogonal array experiments were investigated. The best fermentation conditions for PUFA production were found as follows: 15 % sugarcane molasses, pH=6.0, 28 °C, 5 days, 160 rpm. It was also found that molasses and urea enhanced PUFA production with the optimal carbon to nitrogen (C/N) ratio of 35. Under the most favourable conditions, the total lipid content at 7.13 g/L and PUFA up to 5.74 g/L including (0.82±0.05) g/L of linolenic acid (LA), (1.35±0.02) g/L of γ-linolenic acid (GLA), (0.17±0.06) g/L of α-linolenic acid (ALA), (0.57±0.06) g/L of arachidonic acid (ARA), (0.46±0.07) g/L of eicosapentaenoic acid (EPA) and (0.34±0.08) g/L of docosahexaenoic acid (DHA) were obtained. Our study suggests that sugarcane molasses is a superior alternative carbon source for industrial PUFA production.

Published
2008

2. Synthesis and crystal structure of trans-tetraaqua-bis(3-(((7-hydroxy-3-(4-hydroxy-3-sulfonatophenyl)-4-oxo-4H-chromen-8-yl)methyl)ammonio)propanoato-κO)zinc(II) tetrahydrate, C38H48N2O26S2Zn

Author

Peng Zhang, Hai-Lin Chen, Yan-Jun Xie, Yong-Ling Qin, and Li-Wei Pan

Subjects
Tetrahydrate, 010405 organic chemistry, chemistry.chemical_element, Zinc, Crystal structure, Condensed Matter Physics, 01 natural sciences, Medicinal chemistry, 0104 chemical sciences, Inorganic Chemistry, 010404 medicinal & biomolecular chemistry, chemistry.chemical_compound, chemistry, General Materials Science
Abstract

C38H48N2O26S2Zn, monoclinic, P21/c (no. 14), a = 15.7219(7) Å, b = 8.0394(4) Å, c = 17.1968(8) Å, β = 92.210(4)°, V = 2171.97(18) Å3, Z = 2, Rgt(F) = 0.0395, wRref(F2) = 0.0951, T = 293(2) K.

Published
2018

3. Synthesis and crystal structure poly[aqua(μ3-2-(((7-hydroxy-3-(4-hydroxy-3-sulfonatophenyl)-4-oxo-4H-chromen-8-yl)methyl)ammonio)acetate-κ4 O,O′:O′′:O′′′) sodium] monohydrate, C18H18NNaO11S

Author

Ting Chen, Hong-Fang Lai, Xiao-Yuan Huang, Hai-Lin Chen, Yong-Ling Qin, and Yue-Ning Guo

Subjects
Inorganic Chemistry, 010405 organic chemistry, Chemistry, General Materials Science, Crystal structure, 010403 inorganic & nuclear chemistry, Condensed Matter Physics, 01 natural sciences, Medicinal chemistry, SODIUM MONOHYDRATE, 0104 chemical sciences
Abstract

C18H18NNaO11S, triclinic, P1̅ (no. 2), a = 8.2627(12) Å, b = 8.6490(11) Å, c = 14.1791(17) Å, α = 82.883(10)°, β = 76.954(12)°, γ = 69.729(12)°, V = 924.8(2) Å3, Z = 2, R gt(F) = 0.0574, wR ref(F 2) = 0.1609, T = 293(2) K.

Published
2018

4. Research on the strong transglycosylation activity in Aspergillus niger

Author

Yu-Yan Liu, Zhiqun Liang, Yong-Ling Qin, Yunkai Zhang, and Lan Yu

Subjects
chemistry.chemical_classification, Strain (chemistry), biology, Aspergillus niger, Isomalto oligosaccharides, Plant Science, General Chemistry, medicine.disease_cause, biology.organism_classification, General Biochemistry, Genetics and Molecular Biology, Enzyme, chemistry, Biochemistry, medicine, Escherichia coli
Abstract

Aspergillus niger M-1 strain shows strong transglycosylation activity. A gene of it was introduced into Escherichia coli, and isomalto-oligosaccharides were isolated by a chemical enzymatic method in order to measure the transglycosylation activity.

Published
2008

5. Proizvodnja polinezasićenih masnih kiselina s pomoću plijesni Mucor recurvus sp. upotrebom melase šećerne trske kao izvora ugljika

Author

Nan Li, Zhi-Nian Deng, Yong-Ling Qin, Chun-Lan Chen, and Zhi-Qun Liang

Subjects
Mucor recurvus sp, polinezasićene masne kiseline, melasa šećerne trske, fermentacija, uvjeti uzgoja, analiza masnih kiselina, food and beverages, lipids (amino acids, peptides, and proteins), polyunsaturated fatty acids, sugarcane molasses, fermentation, cultivation condition, fatty acid analysis
Abstract

Sugarcane molasses is rich in nutriments and vitamins. It may be used as the carbon source for the production of polyunsaturated fatty acids (PUFA) by Mucor recurvus sp. Using sugarcane molasses, the effects of fermentation parameters and media components on polyunsaturated fatty acid production through both linear and orthogonal array experiments were investigated. The best fermentation conditions for PUFA production were found as follows: 15 % sugarcane molasses, pH=6.0, 28 °C, 5 days, 160 rpm. It was also found that molasses and urea enhanced PUFA production with the optimal carbon to nitrogen (C/N) ratio of 35. Under the most favourable conditions, the total lipid content at 7.13 g/L and PUFA up to 5.74 g/L including (0.82±0.05) g/L of linolenic acid (LA), (1.35±0.02) g/L of γ-linolenic acid (GLA), (0.17±0.06) g/L of α-linolenic acid (ALA), (0.57±0.06) g/L of arachidonic acid (ARA), (0.46±0.07) g/L of eicosapentaenoic acid (EPA) and (0.34±0.08) g/L of docosahexaenoic acid (DHA) were obtained. Our study suggests that sugarcane molasses is a superior alternative carbon source for industrial PUFA production., Melasa šećerne trske je bogata hranjivim tvarima i vitaminima. Upotrebljava se kao izvor ugljika za proizvodnju polinezasićenih masnih kiselina s pomoću plijesni Mucor recurvus sp. Linearnom i ortogonalnom metodom istražen je utjecaj uvjeta fermentacije i sastojaka podloge, uz dodatak melase šećerne trske, na proizvodnju polinezasićenih masnih kiselina. Najbolji uvjeti fermentacije za proizvodnju polinezasićenih masnih kiselina bili su: 15 % melase šećerne trske; pH=6,0; 28 °C; 5 dana i 160 rpm. Također je utvrđeno da dodatak melase i uree povećava proizvodnju pri optimalnom omjeru ugljika i dušika C/N=35. Pri optimalnim uvjetima proizvedeno je ukupno 7,13 g/L lipida i 5,74 g/L polinezasićenih masnih kiselina, od toga (0,82±0,05) g/L linolenske kiseline, (1,35±0,02) g/L γ-linolenske kiseline, (0,17±0,06) g/L α-linolenske kiseline, (0,57±0,06) g/L arahidonske kiseline, (0,46±0,07) g/L eikosapentenoične kiseline i (0,34±0,08) g/L dokosaheksaenoične kiseline. Ovo je istraživanje pokazalo da je melasa šećerne trske najbolji alternativni izvor ugljika za industrijsku proizvodnju polinezasićenih masnih kiselina.

Published
2008

6. [Screening of microorganism producing polyunsaturated fatty acids with sugarcane molasses]

Author

Nan, Li, Zhi-nian, Deng, Yong-ling, Qin, Min, Ling, and Zhi-qun, Liang

Subjects
Chromatography, Gas, Fatty Acids, Unsaturated, Mucorales, Temperature, Molasses, Hydrogen-Ion Concentration, Polymerase Chain Reaction, Saccharum
Abstract

A strain name LB1 producing polyunsaturated fatty acids with sugarcane molasses was screened form soil samples by the methods of Sudan Black B stain and the determination of PUFAs' iodine value. Based on the grass morphological and physiological characteristics and the sequence similarity of ribosomal DNA-ITS, the strain was identified as Mucor recurvus sp.. The results of L64 (4(3)) orthogonal experiments indicated when Mucor recurvus was cultivated at 28 degrees C for 5 days with 160 r/min, the yields of PUFAs with 10(0) BX sugarcane molasses (pH 6.0) were up to 80.49% content of total fatty acids. The total lipids content was 57.08% of fermentation biomass, which were composed of 15.42% Oleic acid, 14.38% Linolenic, 23.55% gamma-Linolenic, 3.06% alpha-Linolenic, 9.87% Arachidonic acid, 8.14% Eicosapentaenoic acid, 6.07% Docosahexaenoic acid, etc.

Published
2006

7. Production of Polyunsaturated Fatty Acids by Mucor recurvus sp. with Sugarcane Molasses as the Carbon Source

Author

Nan Li, Zhi-Nian Deng, Yong-Ling Qin, Chun-Lan Chen, Zhi-Qun Liang, Nan Li, Zhi-Nian Deng, Yong-Ling Qin, Chun-Lan Chen, and Zhi-Qun Liang

Abstract

Sugarcane molasses is rich in nutriments and vitamins. It may be used as the carbon source for the production of polyunsaturated fatty acids (PUFA) by Mucor recurvus sp. Using sugarcane molasses, the effects of fermentation parameters and media components on polyunsaturated fatty acid production through both linear and orthogonal array experiments were investigated. The best fermentation conditions for PUFA production were found as follows: 15 % sugarcane molasses, pH=6.0, 28 °C, 5 days, 160 rpm. It was also found that molasses and urea enhanced PUFA production with the optimal carbon to nitrogen (C/N) ratio of 35. Under the most favourable conditions, the total lipid content at 7.13 g/L and PUFA up to 5.74 g/L including (0.82±0.05) g/L of linolenic acid (LA), (1.35±0.02) g/L of γ-linolenic acid (GLA), (0.17±0.06) g/L of α-linolenic acid (ALA), (0.57±0.06) g/L of arachidonic acid (ARA), (0.46±0.07) g/L of eicosapentaenoic acid (EPA) and (0.34±0.08) g/L of docosahexaenoic acid (DHA) were obtained. Our study suggests that sugarcane molasses is a superior alternative carbon source for industrial PUFA production., Melasa šećerne trske je bogata hranjivim tvarima i vitaminima. Upotrebljava se kao izvor ugljika za proizvodnju polinezasićenih masnih kiselina s pomoću plijesni Mucor recurvus sp. Linearnom i ortogonalnom metodom istražen je utjecaj uvjeta fermentacije i sastojaka podloge, uz dodatak melase šećerne trske, na proizvodnju polinezasićenih masnih kiselina. Najbolji uvjeti fermentacije za proizvodnju polinezasićenih masnih kiselina bili su: 15 % melase šećerne trske; pH=6,0; 28 °C; 5 dana i 160 rpm. Također je utvrđeno da dodatak melase i uree povećava proizvodnju pri optimalnom omjeru ugljika i dušika C/N=35. Pri optimalnim uvjetima proizvedeno je ukupno 7,13 g/L lipida i 5,74 g/L polinezasićenih masnih kiselina, od toga (0,82±0,05) g/L linolenske kiseline, (1,35±0,02) g/L γ-linolenske kiseline, (0,17±0,06) g/L α-linolenske kiseline, (0,57±0,06) g/L arahidonske kiseline, (0,46±0,07) g/L eikosapentenoične kiseline i (0,34±0,08) g/L dokosaheksaenoične kiseline. Ovo je istraživanje pokazalo da je melasa šećerne trske najbolji alternativni izvor ugljika za industrijsku proizvodnju polinezasićenih masnih kiselina.

Published
2008

8. A high-throughput method for screening of Aspergillus niger mutants with high transglycosylation activity by detecting non-fermentable reducing sugar.

Author

Gui-guang Chen, Wei Li, Yun-kai Zhang, Yong-ling Qin, Kong-yang Wu, and Zhi-qun Liang

Subjects
ASPERGILLUS, GUIZOTIA abyssinica, GENETIC mutation, YEAST, SACCHAROMYCES, SUGARS, CASSAVA, STARCH, FERMENTATION
Abstract

novel high-throughput method was established for rapid screening of large numbers of Aspergillus niger mutants with high transglucosylation activity by exploiting that yeast can hardly hydrolyze isomaltooligosaccharides (IMO). Supernatants of A. niger fermentation were incubated with Saccharomyces cerevisiae to remove glucose and maltose, and the remaining non-reducing sugars, which is positively correlated with the amount of IMO, the products of transglucosylation reaction, were used as indicator of transglucosidase activity of A. niger and examined by dinitrosalicylic acid assay. Using this method, 15 stains that could convert liquefied cassava starch to IMO more efficiently were selected from 8721 A. niger mutants. Among them, mutant C-6181 strain had transglycosidase activity of 4.61 U/ml (increased by 122% compared to its parental strain) and IMO yield of 83.7%. Taking together, the method is easy, simple, efficient and cheap, and has great application potential in selection of transglucosidase-producing strains used in industrial IMO fermentation. [ABSTRACT FROM AUTHOR]

Published
2011
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