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Abstract The immune escape of Omicron variants significantly subsides by the third dose of an mRNA vaccine. However, it is unclear how Omicron variant-neutralizing antibodies develop under repeated vaccination. We analyze blood samples from 41 BNT162b2 vaccinees following the course of three injections and analyze their B-cell receptor (BCR) repertoires at six time points in total. The concomitant reactivity to both ancestral and Omicron receptor-binding domain (RBD) is achieved by a limited number of BCR clonotypes depending on the accumulation of somatic hypermutation (SHM) after the third dose. Our findings suggest that SHM accumulation in the BCR space to broaden its specificity for unseen antigens is a counterprotective mechanism against virus variant immune escape.

Abstract Background While there is a high burden of methicillin-resistant Staphylococcus aureus (MRSA) infections among pediatric patients, studies on the molecular epidemiology of MRSA infections in Korean children since the 2010s are lacking. This study aimed to investigate the molecular genotypes and clinical characteristics of MRSA isolates from children with MRSA bacteremia at Asan Medical Center Children’s Hospital from 2016 to 2021. Methods Clinical data were retrospectively reviewed, and the molecular types of MRSA were determined using multilocus sequence typing (MLST) and Staphylococcal cassette chromosome mec (SCCmec) typing. Results The overall methicillin resistance rate of S. aureus bacteremia was 44.8% (77/172); 49.5% in the period 2016–2018 (period 1) and 37.3% in the period 2019–2021 (period 2) (P = 0.116). Community-acquired infections accounted for only 3.9% of cases. The predominant ST group was ST72 group (67.6%), followed by ST5 group (18.9%) and ST1 group (5.4%). The proportion of ST5 was significantly lower in period 2 compared to period 1 (P = 0.02). Compared to the ST5 and ST1 groups, the ST72 group exhibited lower overall antibiotic resistance and multidrug-resistant (MDR) rates (12.0% [6/50] in ST72 group vs. 100.0% [14/14] in ST5 group vs. 50.0% [2/4] in ST1 group; P

Introduction: The coronavirus disease 2019 (COVID-19) pandemic may have influenced the prevalence and seasonality of acute respiratory viral infections. The aim of the study was to investigate the prevalence of all viruses causing acute viral respiratory infections before and after social distancing measures were lifted. Methods: Cross-sectional study where outpatients and inpatients at Kyunghee University Hospital were examined. From January 2021 to December 2022, respiratory samples were analyzed using multiplex reverse transcriptase real-time polymerase chain reaction. Results: Of 3953 samples obtained, 412 (10.42%) were positive for acute respiratory viral infection, and 502 viruses were detected. The number of viral infections increased from 184 in 2021 to 318 in 2022. Human metapneumovirus was detected from August to November 2022. Human bocavirus (HBoV) was frequently detected from April to June 2021; however, in 2022, HBoV was frequently detected from July to October. Human parainfluenza virus 3 was rarely detected after its initial frequent detection from October to December 2021 but was continuously observed after frequent detection in September 2022. Co-infection occurred in 78 (18.9%) cases. The most common combination of simultaneous infections was human rhinovirus–HBoV (n = 30, 38.5%). Conclusions: During the COVID-19 pandemic, the incidence of acute respiratory viral infection decreased significantly but increased in 2022 when measures were lifted. The prevalence and seasonality of respiratory viral infections have changed since the pandemic. Our findings contribute to the prediction of an effective response to changes in the prevalence of respiratory viruses.

Abstract Background Neuromyelitis optica spectrum disorder (NMOSD) stands out among CNS inflammatory demyelinating diseases (CIDDs) due to its unique disease characteristics, including severe clinical attacks with extensive lesions and its association with systemic autoimmune diseases. We aimed to investigate whether characteristics of B cell receptors (BCRs) differ between NMOSD and other CIDDs using high-throughput sequencing. Methods From a prospective cohort, we recruited patients with CIDDs and categorized them based on the presence and type of autoantibodies: NMOSD with anti-aquaporin-4 antibodies, myelin oligodendrocyte glycoprotein antibody-associated disease (MOGAD) with anti-myelin oligodendrocyte glycoprotein antibodies, double-seronegative demyelinating disease (DSN), and healthy controls (HCs). The BCR features, including isotype class, clonality, somatic hypermutation (SHM), and the third complementarity-determining region (CDR3) length, were analyzed and compared among the different disease groups. Results Blood samples from 33 patients with CIDDs (13 NMOSD, 12 MOGAD, and 8 DSN) and 34 HCs were investigated for BCR sequencing. Patients with NMOSD tended to have more activated BCR features compare to the other disease groups. They showed a lower proportion of unswitched isotypes (IgM and IgD) and a higher proportion of switched isotypes (IgG), increased clonality of BCRs, higher rates of SHM, and shorter lengths of CDR3. Notably, advanced age was identified as a clinical factor associated with these activated BCR features, including increased levels of clonality and SHM rates in the NMOSD group. Conversely, no such clinical factors were found to be associated with activated BCR features in the other CIDD groups. Conclusions NMOSD patients, among those with CIDDs, displayed the most pronounced B cell activation, characterized by higher levels of isotype class switching, clonality, SHM rates, and shorter CDR3 lengths. These findings suggest that B cell-mediated humoral immune responses and characteristics in NMOSD patients are distinct from those observed in the other CIDDs, including MOGAD. Age was identified as a clinical factor associated with BCR activation specifically in NMOSD, implying the significance of persistent B cell activation attributed to anti-aquaporin-4 antibodies, even in the absence of clinical relapses throughout an individual’s lifetime.

The assessment of spatial variability in the ground through field experiments has many constraints due to non-homogenous ground conditions and lack of site investigations. For this reason, spatial variability has not been considered in typical analyses. Also, few studies have been conducted on ground spatial variability using models in the laboratory. However, it is necessary to evaluate spatial variability in the ground for more precise analysis and design of construction projects. In this study, in order to evaluate spatial variability in the ground, we performed a number of Mini-Cone Penetration Tests (CPTs) in ground models with one layer of silica sand soil and two layers of silica and weathered soils. Through the Mini-CPTs, cone penetration resistances with depth were measured at many points. Based on the data, the coefficient of variation (CV) and the correlation length (CL) were calculated to quantitatively analyze the vertical and horizontal variability in the ground models. The results showed how the spatial variability in the two ground models varied. This implies that considering spatial variability in the ground can significantly enhance the accuracy of the analysis and design of construction projects.

Background: Korean Red Ginseng is a major source of bioactive substances such as ginsenosides. Efficacy of red ginseng extract (RGE), which contains not only saponins but also various non-saponins, has long been studied. In the water-soluble component-rich fraction of RGE (WS), a byproduct generated in the process of extracting saponins from the RGE, we identified previously unidentified molecules and confirmed their efficacy. Methods: The RGE was prepared and used to produce WS, whose components were isolated sequentially according to their water affinity. The new compounds from WS were fractionized and structurally analyzed using nuclear magnetic resonance spectroscopy. Physiological applicability was evaluated by verifying the antioxidant and anti-inflammatory efficacies of these compounds in vitro. Results: High-performance liquid chromatography confirmed that the obtained WS comprised 11 phenolic acid and flavonoid substances. Among four major compounds from fractions 1−4 (F1–4) of WS, two compounds from F3 and F4 were newly identified in red ginseng. The analysis results show that these compound molecules are member of the maltol-structure-based glucopyranose series, and F1 and F4 are particularly effective for decreasing oxidative stress levels and inhibiting nitric oxide secretion, interleukin (IL)-1β and IL-6, and tumor necrosis factor-α. Conclusion: Our findings suggest that a few newly identified maltol derivatives, such as red ginseng-derived non-saponin in the WS, exhibit antioxidant and anti-inflammatory effects, making them viable candidates for application to pharmaceutical, cosmetic, and functional food materials.

Abstract This study introduces a new chemistry option in the Weather Research and Forecasting model data assimilation (WRFDA) system, coupled with the WRF‐Chem model (Version 4.4.1), to incorporate aqueous chemistry (AQCHEM) in the assimilation of ground‐level chemical measurements. The new DA capability includes the integration of aqueous‐phase aerosols from the Regional Atmospheric Chemistry Mechanism (RACM) gas chemistry, the Modal Aerosol Dynamics Model for Europe (MADE) aerosol chemistry, and the Volatility Basis Set (VBS) for secondary organic aerosol production. The RACM‐MADE‐VBS‐AQCHEM scheme facilitates aerosol‐cloud‐precipitation interactions by activating aerosol particles in cloud water during the model simulation. With the goal of enhancing air quality forecasting in cloudy conditions, this new implementation is demonstrated in the weakly coupled three‐dimensional variational data assimilation (3D‐Var) system through regional air quality cycling over East Asia. Surface particulate matter (PM) concentrations and four gas species (SO2, NO2, O3, and CO) are assimilated every 6 hr for the month of March 2019. The results show that including aqueous‐phase aerosols in both the analysis and forecast can represent aerosol wet removal processes associated with cloud development and rainfall production. During a pollution event with high cloud cover, simulations without aerosols defined in cloud water exhibit significantly higher values for liquid water path, and surface PM10 (PM2.5) concentrations are overestimated by a factor of 10 (3) when wet scavenging processes dominate. On the contrary, AQCHEM proves to be helpful in simulating the wet deposition of aerosols, accurately predicting the evolution of surface PM concentrations without such overestimation.

Myasthenia Gravis (MG) patients with anti-acetylcholine receptor (AChR) antibodies frequently show hyperplastic thymi with ectopic germinal centers, where autoreactive B cells proliferate with the aid of T cells. In this study, thymus and peripheral blood (PB) samples were collected from ten AChR antibody-positive MG patients. T cell receptor (TCR) repertoires were analyzed using next-generation sequencing (NGS), and compared with that of an age and sex matched control group generated from a public database. Certain V genes and VJ gene recombination pairs were significantly upregulated in the TCR chains of αβ-T cells in the PB of MG patients compared to the control group. Furthermore, the TCR chains found in the thymi of MG patients had a weighted distribution to longer CDR3 lengths when compared to the PB of MG patients, and the TCR beta chains (TRB) in the MG group's PB showed increased clonality encoded by one upregulated V gene. When TRB sequences were sub-divided into groups based on their CDR3 lengths, certain groups showed decreased clonality in the MG group's PB compared to the control group's PB. Finally, we demonstrated that stereotypic MG patient-specific TCR clonotypes co-exist in both the PB and thymi at a much higher frequency than that of the clonotypes confined to the PB. These results strongly suggest the existence of a biased T cell-mediated immune response in MG patients, as observed in other autoimmune diseases.

PURPOSE The purpose of this study was to develop an estimation equation that predicts energy expenditure (EE) during swimming exercise based on heart rate (HR). And, we also evaluated the validation for field application and compare it with the existing speed-based swimming EE equation. METHODS A total of 63 healthy adults (ages 19-49 years) who could swim at various speeds were conveniently sampled. The experi-ment was conducted in an indoor 25 m pool. The protocol (measurement of individual best performance, 0.4, 0.6, 0.8,

Abstract Background Much of researchers’ efforts to foster wider implementation of educational innovations in STEM has focused on understanding and facilitating the implementation efforts of faculty. However, student engagement in blended learning and other innovations relies heavily on students’ self-directed learning behaviors, implying that students are likely key actors in the implementation process. This paper explores the ways in which engineering students at multiple institutions experience the self-directed selection and implementation of blended learning resources in the context of their own studies. To accomplish this, it adopts a research perspective informed by Actor-Network Theory, allowing students themselves to be perceived as individual actors and implementors rather than a population that is implemented upon. Results A thematic analysis was conducted in two parts. First, analysis identified sets of themes unique to the student experience at four participant institutions. Then, a second round of analysis identified and explored a subset of key actors represented in students’ reported experiences across all institutions. The findings show clear similarities and differences in students’ experiences of blended learning across the four institutions, with many themes echoing or building upon the results of prior research. Distinct institutional traits, the actions of the instructors, the components of the blended learning environment, and the unique needs and preferences of the students themselves all helped to shape students’ self-directed learning experiences. Students’ engagement decisions and subsequent implementations of blended learning resulted in personally appropriate, perhaps even idiosyncratic, forms of engagement with their innovative learning opportunities. Conclusion The institutional implementation of blended learning, and perhaps other educational innovations, relies in part on the self-directed decision-making of individual students. This suggests that instructors too hold an additional responsibility: to act as facilitators of their students’ implementation processes and as catalysts for growth and change in students’ learning behaviors. Developing a greater understanding of students’ implementation behaviors could inform the future implementation efforts of faculty and better empower students to succeed in the innovative classroom.

PURPOSE This study aimed to develop age- and sex-specific smoothed percentile curves for fitness items using LMS methods in Koreans aged 13–85 years or older. METHODS A total of 559,430 individuals aged 13-85 years or older (adolescents: 13-18 years, adults: 19-64 years; older adults: >65 years) participated in the National Fitness Award (NFA) project in 2017, 2018, and 2019 in Korea. In this study, the results of body composition (body fat percent), muscle strength (absolute handgrip strength and relative handgrip strength), and flexibility (sit and reach) tests performed by adolescents, adults, and older adults, were analyzed. Cardiorespiratory fitness (20-m PACER, VO2 max) was performed only by adolescents and adults. Percentile curves of fitness items smoothed by age- and sex-specific variables were calcu-lated for fit using the LMS method. RESULTS Percentile curves for each age- and sex-specific group were presented for each physical fitness component. Percent body fat in men and women increased from the age of 25 and 30 years, respectively. Flexibility showed a tendency to decrease sharply after the age of 65 and 70 years in men and women, respectively. Muscle strength decreased gradually after 40 years, and sharply after 60 years. Cardiorespiratory fitness tended to decrease from the age of 25 years in men and from 19 years in women. CONCLUSIONS The NFA project dataset provided the opportunity to establish normative values for fitness items in Korean men and women aged 13-85 years or older. The use of the LMS method enabled factoring age in the analysis. Normative reference values of fitness items can be used to interpret individuals’ fitness levels during the everyday practice of fitness and/or in clinical settings.

B cells recognize antigens via membrane‐expressed B‐cell receptors (BCR) and antibodies. Similar human BCR sequences are frequently found at a significantly higher frequency than that theoretically calculated. Patients infected with SARS‐CoV2 and HIV or with autoimmune diseases share very similar BCRs. Therefore, in silico reconstitution of BCR repertoires and identification of stereotypical BCR sequences related to human pathology have diagnostic potential. Furthermore, monitoring changes of clinically significant BCR sequences and isotype conversion has prognostic potential. For BCR repertoire analysis, peripheral blood (PB) is the most convenient source. However, the optimal human PB volume for in silico reconstitution of the BCR repertoire has not been studied in detail. Here, we sampled 5, 10, and 20 mL PB from the left arm and 40 mL PB from the right arm of two volunteers, reconstituted in silico PB BCR repertoires, and compared their composition. In both volunteers, PB sampling over 20 mL resulted in slight increases in functional unique sequences (FUSs) or almost no increase in repertoire diversity. All FUSs with a frequency above 0.08% or 0.03% in the 40 mL PB BCR repertoire were detected even in the 5 mL PB BCR repertoire from each volunteer. FUSs with a higher frequency were more likely to be found in BCR repertoires from reduced PB volume, and those coexisting in two repertoires showed a statistically significant correlation in frequency irrespective of sampled anatomical site. The correlation was more significant in higher‐frequency FUSs. These observations support the potential of BCR repertoire analysis for diagnosis.

The preparation of quantum silicon nanowires, materials with potential application in high-performance nanodevices, is challenging. Here, the authors synthesize vertically aligned sub-5 nm silicon nanowires via a vapor phase silicon etching process; the resulting material features unusual lattice reduction and significant phonon and electronic confinement effects.

The spatial context of epitranscriptomic features in the tumour microenvironment remains poorly understood. Here, a method for transcriptomic and epitranscriptomic analysis of immunofluorescence-stained tissue, Select-seq, is applied to stem cell-like microniches in triple negative breast cancer.

In this work, the guided wave de-bonding area-detecting technique was studied for application to containment liner plates in nuclear power plant areas. To apply this technique, an appropriate Lamb wave mode, symmetric and longitudinal dominance, was verified by the frequency shifting technique. The S0 2.7 MHz mm Lamb wave mode was chosen to realize quantitative experimental results and their visualization. Results of the bulk wave, longitudinal wave mode, and comparison experiments indicate that the wave mode was able to distinguish between the de-bonded and bonded areas. Similar to the bulk wave cases, the bonded region could be distinguished from the de-bonded region using the Lamb wave approach. The Lamb wave technique results showed significant correlation to the de-bonding area. As the de-bonding area increased, the Lamb wave energy attenuation effect decreased, which was a prominent factor in the realization of quantitative tomographic visualization. The feasibility of tomographic visualization was studied via the application of Lamb waves. The reconstruction algorithm for the probabilistic inspection of damage (RAPID) technique was applied to the containment liner plate to verify and visualize the de-bonding condition. The results obtained using the tomography image indicated that the Lamb wave-based RAPID algorithm was capable of delineating debonding areas.

Abstract Background Physical therapy treatments incorporating equine movement are recognized as an effective tool to treat functional mobility and balance in children with cerebral palsy (CP). To date, only a few studies examined kinematic outputs of the horses and children when mounted. In this pilot study, to better understand the effectiveness of this type of treatment, we examined the interaction between the horses and children with CP during physical therapy sessions where equine movement was utilized. Methods Four children with CP participated in eight physical therapy sessions incorporating hippotherapy as a treatment intervention. Functional mobility was assessed using the Timed Up Go or the 10 m Walk Test. Inertial measurement unit sensors, attached to children and horses, recorded movements and tracked acceleration, angular velocity, and body orientation. Correlation between vertical accelerations of children and horses were analyzed. In addition, peak frequencies of vertical accelerations of children and horses were compared. Results Functional tests modestly improved over time. The children’s movements, (quantified in frequency and temporal domains) increasingly synchronized to the vertical movement of the horse’s walk, demonstrated by reduced frequency errors and increased correlation. Conclusions The findings suggest that as the sessions progressed, the participants appeared to become more familiar with the horse’s movement. Since the horse’s gait at a walk mimics the human gait this type of treatment may provide individuals with CP, who have abnormal gait patterns, an opportunity for their neuromuscular system to experience a typical gait pattern. The horse’s movement at the walk are consistent, cyclical, rhythmical, reciprocal and multi-dimensional, all of which can facilitate motor learning. The increased synchronization between horse and the mounted participant suggests that physical therapy utilizing equine movement is a viable treatment tool to enhance functional mobility. This study may provide a useful baseline for future work. Trial registrationTexas A&M University Institutional Review Board. IRB2018-0064. Registered 8 March 2018. Link: https://rcb.tamu.edu/humans/irb and https://github.com/pilwonhur/HPOT

Composite materialsuch as glass-fiber reinforced plastic and carbon-fiber reinforced plastic (CFRP) shows anisotropic property and have been widely used for structural members and outfitings of ships. The structural safety of composite structures has been generally evaluated via finite element analysis. This paper presents a technique for updating the finite element model of anisotropic beams or plates via natural frequencies. The finite element model updates involved a compensation process of anisotropic material properties, such as the elastic and shear moduli of orthotropic structural members. The technique adopted was based on a discrete genetic algorithm, which is an optimization technique. The cost function was adopted to assess the optimization problem, which consisted of the calculated and referenced low-order natural frequencies for the target structure. The optimization process was implemented with MATLAB, which includes the finite element updates and the corresponding natural frequency calculations with MSC/NASTRAN. Material properties of a virtual cantilevered orthotropic beam were estimated to verify the presented method and the results obtained were compared with the reference values. Furthermore, the technique was applied to a cantilevered CFRP beam to successfully estimate the unknown material properties.

Next generation sequencing has difficulty in detecting rare-frequency variants due to high sequencing errors. Here the authors present a barcode-free error validation method that physically extracts erroneous reads to identify true variants.

Abstract Introduction The basic paradigm of rehabilitation is based on the brain plasticity, and for promoting it, test–retest reliability (TRR) of brain activation in which certain area of the brain is repeatedly activated is required. In this study, we investigated whether the robotic passive movement has the TRR of brain activation. While active training has been shown to have TRR, but there still have been arguments over the TRR by passive movement. Methods In order to test TRR, 10 repetitive sessions and various intervals (1 day, 3 days, 7 days, 23 days, 15 min, and 6 hr) were applied to five subjects, which had the same statistical power as applying two sessions to 50 subjects. In each session, three robot speeds (0.25, 0.5, and 0.75 Hz) were applied to provide passive movement using the robot. The fNIRS signal (oxy‐Hb) generated in the primary sensorimotor area (SM1) was measured on a total of 29 channels. At this time, we used activation maps and intraclass correlation coefficient (ICC) values to examine the TRR and the effect of robot speeds and intervals on TRR. Results As a result, activation maps showed prominent variation regardless of robot speeds and interval, and the ICC value (=0.002) showed no TRR of brain activation for robotic passive movement. Conclusion The brain activation induced by the robotic passive movement alone has very poor TRR, suggesting that further enhancement is required to strengthen the TRR by complementing active user engagements.

A series of two-dimensional finite element analyses are performed to simulate the seismic response of slope composed of granular soil. Four sets of input parameters for the nonlinear soil model are used to fit the reference the shear modulus reduction and damping curves, thereby to evaluate the influence of the nonlinear soil model. The first set is fitted to the shear modulus reduction curve. The second and third sets are fitted simultaneously to both shear modulus reduction and damping curves. The final set applied the shear strength adjustment to adequately capture the nonlinear soil response at large strains. The accuracy of each set of parameters are evaluated through comparison with centrifuge model test measurements. It is observed that the nonlinear soil model has a marginal influence on the acceleration response. On the contrary, the vertical settlement is highly influenced by the nonlinear soil model. The discrepancy is shown to increase with an increase in the intensity of the input ground motion. It is demonstrated that the adjustment for the shear strength is important in performing seismic analyses of slopes, which is most often ignored in practice. Based on the results, practical guidelines on how to select the parameters for the nonlinear soil model are provided.

The seismic performance of slopes is typically evaluated with a pseudo-static method using equivalent horizontal load or with Newmark sliding block analysis. In both procedures, the definition of the potential sliding surface is a required input. The sliding surface has been reported to be marginally influenced by the input ground motion and, therefore, is most often assumed from a pseudo-static procedure. In this study, extensive series of two-dimensional dynamic nonlinear finite element analyses are performed to evaluate the sensitivity of the sliding surface on the slope geometry, soil strength parameters, and input ground motion characteristics. It is demonstrated that the sliding surface may vary with the intensity and frequency characteristics of the input motion. Slopes with inclination angle equal or less than 35° are shown to be marginally influenced by motion intensity if the mean period (Tm) < 0.3 s. However, slopes inclined at 45° are revealed to be more sensitive to the motion intensity and Tm. For motions with Tm > 0.3 s, the sliding surface is demonstrated to widen with an increase in the intensity of the input ground motions. The degree of widening increases proportionally with an increase in Tm. It is, therefore, recommended to derive sliding surfaces from a dynamic analysis for steep slopes.

In this study, a modified imaging algorithm was implemented to improve the imaging accuracy for defects located on a structure. Based on analysis of the Lamb wave mode, a guided ultrasonic wave inspection technique was applied, which was able to illustrate images of defects in a 6 mm steel plate simulating containment liner plate (CLP) in nuclear power plants. The dominant Lamb wave mode was determined through short-time Fourier transform waveform analysis and imaging verification. Following tomography verification, limitations of the antisymmetric mode in the thick steel plate were identified. In addition, a modified shape factor, based on the energy distribution factor according to the beam pattern and beam width, was suggested for field applications and improved imaging accuracy. Results of the analysis revealed a beam skewing phenomenon for the Lamb wave mode. In the case of S0 2.7 MHz·mm, skewing as well as distortion effects are not observed in the experiment, while the S0 modes at 2.64 and 2.74 MHz·mm show either of them. Considering skewing width, the size of the shape function was modified. Application of the modified shape function allows us to obtain more accurate image to actual defect shape.

Abstract Finding suitable electrode materials is one of the challenges for the commercialization of a sodium ion battery due to its pulverization accompanied by high volume expansion upon sodiation. Here, copper sulfide is suggested as a superior electrode material with high capacity, high rate, and long‐term cyclability owing to its unique conversion reaction mechanism that is pulverization‐tolerant and thus induces the capacity recovery. Such a desirable consequence comes from the combined effect among formation of stable grain boundaries, semi‐coherent boundaries, and solid‐electrolyte interphase layers. The characteristics enable high cyclic stability of a copper sulfide electrode without any need of size and morphological optimization. This work provides a key finding on high‐performance conversion reaction based electrode materials for sodium ion batteries.

In order to verify the reliability of numerical analysis, a series of 1 g shaking table tests for flat ground and slope were conducted using a laminar shear box subjected to different seismic waves. Firstly, numerical analyses, using the DEEPSOIL and ABAQUS software, were done to compare the results of flat ground experiments. After that, finite element analyses with ABAQUS were conducted to compare the results of slope experiments. For numerical analyses, considering the influence of the boundary, the concept of adjusted elastic modulus was proposed to improve the simulation results. Based on the analyses, it is found that in terms of acceleration-time history and spectral acceleration, the numerical analysis results are in good agreement with the experiment results. This implies that numerical analysis can capture the dynamic behavior of soil under 1 g shaking table test conditions.

In order to evaluate the effects of soil box boundary conditions on the dynamic soil behavior, the Rigid Box (RB) and the Laminar Shear box (LSB) were constructed and 1 g shaking table tests were carried out for various boundary conditions. The boundary effects of the RB and the LSB were compared. To reduce the boundary effects of the RB, sponges, 5 cm, 10 cm, and 15 cm in thickness, were attached to the two end sides of the RB. A model soil was constructed on flat ground, and the acceleration and amplification occurring in the center of the soil were analyzed by spectrum and peak ground acceleration. Compared with the RB, the center and wall accelerations of LSB were very close to each other. This implies that the LSB can better simulate the behavior of the infinite half space than the RB.

Recently, various immunosuppressant drugs have been shown to induce hair growth in normal hair as well as in alopecia areata and androgenic alopecia; however, the responsible mechanism has not yet been fully elucidated. In this study, we investigate the influence of mycophenolate (MPA), an immunosuppressant, on the proliferation of human dermal papilla cells (hDPCs) and on the growth of human hair follicles following catagen induction with interferon (IFN)-γ. IFN-γ was found to reduce β-catenin, an activator of hair follicle growth, and activate glycogen synthase kinase (GSK)-3β, and enhance expression of the Wnt inhibitor DKK-1 and catagen inducer transforming growth factor (TGF)-β2. IFN-γ inhibited expression of ALP and other dermal papillar cells (DPCs) markers such as Axin2, IGF-1, and FGF 7 and 10. MPA increased β-catenin in IFN-γ-treated hDPCs leading to its nuclear accumulation via inhibition of GSK3β and reduction of DKK-1. Furthermore, MPA significantly increased expression of ALP and other DPC marker genes but inhibited expression of TGF-β2. Therefore, we demonstrate for the first time that IFN-γ induces catagen-like changes in hDPCs and in hair follicles via inhibition of Wnt/β-catenin signaling, and that MPA stabilizes β-catenin by inhibiting GSK3β leading to increased β-catenin target gene and DP signature gene expression, which may, in part, counteract IFN-γ-induced catagen in hDPCs.

The containment liner plate (CLP) in a nuclear power plant is the most critical part of the structure of a power plant, as it prevents the radioactive contamination of the surrounding area. This paper presents feasibility of structural health monitoring (SHM) and an elastic wave tomography method based on ultrasonic guided waves (GW), for evaluating the integrity of CLP. It aims to check the integrity for a dynamic response to a damaged isotropic structure. The proposed SHM technique relies on sensors and, therefore, it can be placed on the structure permanently and can monitor either passively or actively. For applying this method, a suitable guided wave mode tuning is required to verify wave propagation. A finite element analysis (FEA) is performed to figure out the suitable GW mode for a CLP by considering geometric and material condition. Furthermore, elastic wave tomography technique is modified to evaluate the CLP condition and its visualization. A modified reconstruction algorithm for the probabilistic inspection of damage tomography algorithm is used to quantify corrosion defects in the CLP. The location and shape of the wall-thinning defects are successfully obtained by using elastic GW based SHM. Making full use of verified GW mode to Omni-directional transducer, it can be expected to improve utilization of the SHM based evaluation technique for CLP.

YYB-101 is a humanized rabbit anti-human hepatocyte growth factor (HGF)-neutralizing antibody currently in clinical trial. To test the effect of HGF neutralization with antibody on anti-cancer T cell immunity, we generated surrogate antibodies that are reactive to the mouse homologue of the epitope targeted by YYB-101. First, we immunized a chicken with human HGF and monitored changes in the B cell repertoire by next-generation sequencing (NGS). We then extracted the VH gene repertoire from the NGS data, clustered it into components by sequence homology, and classified the components by the change in the number of unique VH sequences and the frequencies of the VH sequences within each component following immunization. Those changes should accompany the preferential proliferation and somatic hypermutation or gene conversion of B cells encoding HGF-reactive antibodies. One component showed significant increases in the number and frequencies of unique VH sequences and harbored genes encoding antibodies that were reactive to human HGF and competitive with YYB-101 for HGF binding. Some of the antibodies also reacted to mouse HGF. The selected VH sequences shared 98.3% identity and 98.9% amino acid similarity. It is therefore likely that the antibodies encoded by them all react to the epitope targeted by YYB-101.

BACKGROUND: This study aimed to determine the association of lipoprotein-associated phospholipase A(2) (Lp-PLA(2)) activity in circulation and peripheral blood mononuclear cells (PBMCs) with inflammatory and oxidative stress markers in nonobese women and according to menopausal status. Lp-PLA(2) activity, a marker for cardiovascular risk is associated with inflammation and oxidative stress. METHODOLOGY/PRINCIPAL FINDINGS: Eighty postmenopausal women (53.0±4.05 yr) and 96 premenopausal women (39.7±9.25 yr) participated in this study. Lp-PLA(2) activities, interleukin (IL)-6, tumor necrosis factor (TNF)-α, and IL-1β in plasma as well as in PBMCs were measured. Plasma ox-LDL was also measured. Postmenopausal women demonstrated higher circulating levels of ox-LDL and IL-6, as well as IL-6, TNF-α, and IL-1β in PBMCs, than premenopausal women. In both groups, plasma Lp-PLA(2) activity positively correlated with Lp-PLA(2) activity in PBMCs and plasma ox-LDL. In premenopausal women, Lp-PLA(2) activities in plasma and PBMCs positively correlated with IL-6, TNF-α, and IL-1β in PBMCs. In postmenopausal women, plasma ox-LDL positively correlated with PBMC cytokine production. In subgroup analysis of postmenopausal women according to plasma ox-LDL level (median level: 48.715 U/L), a significant increase in Lp-PLA(2) activity in the plasma but not the PBMCs was found in the high ox-LDL subgroup. Plasma Lp-PLA(2) activity positively correlated with unstimulated PBMC Lp-PLA(2) activity in the low ox-LDL subgroup (r = 0.627, P

The quaternary thiophosphate, Cs0.49NbPS6, caesium hexathioniobiophosphate(V), has been synthesized by the reactive halide flux method. The title compound is isotypic with Rb0.46TaPS6 and is made up of a bicapped trigonal–biprismatic [Nb2S12] unit and a tetrahedral [PS4] group. The [Nb2S12] units linked by the [PS4] tetrahedra form infinite chains, yielding a three-dimensional network with rather large van der Waals gaps along the c axis in which the disordered Cs+ ions reside. The electrons released by the Cs atoms are transferred to the pairwise niobium metal site and there are substantial intermetallic Nb—Nb bonding interactions. This leads to a significant decrease of the intermetallic distance in the title compound compared to that in TaPS6. The classical charge balance of the title compound may be represented as [Cs+]0.49[Nb4.51+][P5+][S2−]4[S22−].