160 results on '"Yongjing Zhang"'
Search Results
2. Disruption of TIGAR-TAK1 alleviates immunopathology in a murine model of sepsis
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Dongdong Wang, Yanxia Li, Hao Yang, Xiaoqi Shen, Xiaolin Shi, Chenyu Li, Yongjing Zhang, Xiaoyu Liu, Bin Jiang, Xudong Zhu, Hanwen Zhang, Xiaoyu Li, Hui Bai, Qing Yang, Wei Gao, Fang Bai, Yong Ji, Qi Chen, and Jingjing Ben
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Science - Abstract
Abstract Macrophage-orchestrated inflammation contributes to multiple diseases including sepsis. However, the underlying mechanisms remain to be defined clearly. Here, we show that macrophage TP53-induced glycolysis and apoptosis regulator (TIGAR) is up-regulated in murine sepsis models. When myeloid Tigar is ablated, sepsis induced by either lipopolysaccharide treatment or cecal ligation puncture in male mice is attenuated via inflammation inhibition. Mechanistic characterizations indicate that TIGAR directly binds to transforming growth factor β-activated kinase (TAK1) and promotes tumor necrosis factor receptor-associated factor 6-mediated ubiquitination and auto-phosphorylation of TAK1, in which residues 152-161 of TIGAR constitute crucial motif independent of its phosphatase activity. Interference with the binding of TIGAR to TAK1 by 5Z-7-oxozeaenol exhibits therapeutic effects in male murine model of sepsis. These findings demonstrate a non-canonical function of macrophage TIGAR in promoting inflammation, and confer a potential therapeutic target for sepsis by disruption of TIGAR-TAK1 interaction.
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- 2024
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3. Stretchable hybrid platform‐enabled interactive perception of strain sensing and visualization
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Yikun Liu, Yongju Gao, Beom Jin Kim, Meili Xia, Yunlong Zhou, Yongjing Zhang, Yang Li, Jianying Huang, Duxia Cao, Songfang Zhao, Jong‐Hyun Ahn, and Yuekun Lai
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bioinspired structure ,fluorescence molecule ,interactive perception ,strain sensing ,visualization ,Materials of engineering and construction. Mechanics of materials ,TA401-492 - Abstract
Abstract Human–machine interactive platforms that can sense mechanical stimuli visually and digitally are highly desirable. However, most existing interactive devices cannot satisfy the demands of tactile feedback and extended integration. Inspired by the mechanoluminescence (ML) function of cephalopod skin and the sensitive perception of microcracked slit‐organs, a bioinspired stretchable interactive platform is developed by designing a stretchable poly(styrene‐block‐butadiene‐block‐styrene)/fluorescent molecule (SFM) composite followed by the in situ polymerization of pyrrole (Py) and deposition of carbon nanotubes (CNTs), which possesses a simple multilayered structure and quantitatively senses the applied strains via the variations of digital electrical resistance and visual fluorescence intensity. Using the strain‐dependent microstructures derived from the synergistic interactions of the rigid PPy/CNTs functional layer and SFM, the SFM/PPy/CNTs‐based platforms exhibit excellent strain‐sensing performance manifested by a high gauge factor (GF = 2.64 × 104), wide sensing range (~270%), fast response/recovery time (~155/195 ms), excellent stability (~15,000 cycles at 40% strain), and sensitive ML characteristics under ultraviolet illumination. Benefiting from the novel fusion of digital data and visual images, important applications, including the detection of wrist pulses and human motions, and information dual‐encryption, are demonstrated. This study demonstrates the superiority of advanced structures and materials for realizing superior applications in wearable electronics.
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- 2024
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4. Secure and decentralized federated learning framework with non-IID data based on blockchain
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Feng Zhang, Yongjing Zhang, Shan Ji, and Zhaoyang Han
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Blockchain ,Federated learning ,Smart contract ,Non-IID data ,Privacy preservation ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Federated learning enables the collaborative training of machine learning models across multiple organizations, eliminating the need for sharing sensitive data. Nevertheless, in practice, the data distributions among these organizations are often non-independent and identically distributed (non-IID), which poses significant challenges for traditional federated learning. To tackle this challenge, we present a hierarchical federated learning framework based on blockchain technology, which is designed to enhance the training of non-IID data., protect data privacy and security, and improve federated learning performance. The framework builds a global shared pool by constructing a blockchain system to reduce the non-IID degree of local data and improve model accuracy. In addition, we use smart contracts to distribute and collect models and design a main blockchain to store local models for federated aggregation, achieving decentralized federated learning. We train the MLP model on the MNIST dataset and the CNN model on the Fashion-MNIST and CIFAR-10 datasets to verify its feasibility and effectiveness. The experimental results show that the proposed strategy significantly improves the accuracy of decentralized federated learning on three tasks with non-IID data.
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- 2024
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5. Kirigami‐Structured, Low‐Impedance, and Skin‐Conformal Electronics for Long‐Term Biopotential Monitoring and Human–Machine Interfaces
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Meili Xia, Jianwen Liu, Beom Jin Kim, Yongju Gao, Yunlong Zhou, Yongjing Zhang, Duxia Cao, Songfang Zhao, Yang Li, and Jong‐Hyun Ahn
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bioelectric interfacial impedance ,epidermal electrode ,epidermal electrophysiology ,human–machine interface ,Kirigami structure ,Science - Abstract
Abstract Epidermal dry electrodes with high skin‐compliant stretchability, low bioelectric interfacial impedance, and long‐term reliability are crucial for biopotential signal recording and human–machine interaction. However, incorporating these essential characteristics into dry electrodes remains a challenge. Here, a skin‐conformal dry electrode is developed by encapsulating kirigami‐structured poly(3,4‐ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS)/polyvinyl alcohol (PVA)/silver nanowires (Ag NWs) film with ultrathin polyurethane (PU) tape. This Kirigami‐structured PEDOT:PSS/PVA/Ag NWs/PU epidermal electrode exhibits a low sheet resistance (≈3.9 Ω sq−1), large skin‐compliant stretchability (>100%), low interfacial impedance (≈27.41 kΩ at 100 Hz and ≈59.76 kΩ at 10 Hz), and sufficient mechanoelectrical stability. This enhanced performance is attributed to the synergistic effects of ionic/electronic current from PEDOT:PSS/Ag NWs dual conductive network, Kirigami structure, and unique encapsulation. Compared with the existing dry electrodes or standard gel electrodes, the as‐prepared electrodes possess lower interfacial impedance and noise in various conditions (e.g., sweat, wet, and movement), indicating superior water/motion‐interference resistance. Moreover, they can acquire high‐quality biopotential signals even after water rinsing and ultrasonic cleaning. These outstanding advantages enable the Kirigami‐structured PEDOT:PSS/PVA/Ag NWs/PU electrodes to effectively monitor human motions in real‐time and record epidermal biopotential signals, such as electrocardiogram, electromyogram, and electrooculogram under various conditions, and control external electronics, thereby facilitating human–machine interactions.
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- 2024
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6. Province-level distribution and drivers of infant mortality in mainland China: a Geodetector-based analysis of data from 2020
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Xiao Zhang, Junhui Zhang, Yuwen Tang, Beibei Zhang, Yongjing Zhang, and Jifeng Dai
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Medicine - Abstract
Objective The present study investigated the province-level distribution and drivers of infant mortality rate (IMR) in mainland China.Design Ecological analysis based on publicly available data for all 31 provinces in mainland China.Data sources Data on province-level IMRs in 2020 were obtained from the official websites of the healthcare commissions within each province and from the China Health Statistics Yearbook 2021. Data on potential IMR drivers were retrieved from the China Statistical Yearbook 2021.Data analysis GeoDa V.1.12.1 and ArcMap V.10.2 software were used to examine province-level distribution of IMR. Global and local spatial autocorrelations were performed, and Getis-ord G* hotspots and coldspots were identified. Geodetector was used to analyse the individual and joint influence of drivers on IMR.Results IMRs in 2020 varied from 1.91 to 7.60 per 1000 live births across provinces. The following statistically significant drivers with q values >0.5 were identified: health literacy of the population (0.6673), male illiteracy rate (0.6433), proportion of the population older than >65 years (0.6369), per capita government health expenditure (0.6216), forest coverage rate (0.5820), per capita disposable income (0.5785), per capita number of hospitals (0.5592), per capita gross regional product (0.5410) and sulfur dioxide concentration in the atmosphere (0.5158). The following three interactions among these drivers emerged as strongest influences on province-level IMR: proportion of population >65 years ∩ per capita gross regional product (q=0.9653), forest coverage rate ∩ per capita gross regional product (0.9610) and per capita government health expenditure ∩ sulfur dioxide (0.9295).Conclusion IMR in mainland China varies substantially across the country, being generally high-west and low-east. Several factors, on their own and interacting together, contribute to IMR. Policies and programmes to reduce IMR should be formulated according to local conditions and should focus on western provinces of the country.
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- 2023
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7. Characteristics of systematic lymph node dissection and influencing factors of sentinel lymph node biopsy using carbon nanoparticles in endometrial carcinoma: a single-center study
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Siqi Tao, Zhibang Zhang, Liling Li, Xiaorui Yuan, Hongliang Chen, Yongjing Zhang, and Chun Fu
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Endometrial carcinoma ,Lymph node metastasis ,Sentinel lymph node mapping ,Prediction model ,Surgery ,RD1-811 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background Carbon nanoparticles (CNPs) are a new tracer for lymph node mapping, which can quickly reach and develop lymph nodes through a lymphatic network. This research investigated the characteristics of systematic lymph node dissection and sentinel lymph node biopsy mapped with CNPs in endometrial carcinoma. Methods We first applied CNPs to systematic lymph node dissection in 18 endometrial carcinoma patients as the study group and another 18 endometrial carcinoma patients who were not injected with anything served as the control group. Then, we applied CNPs to sentinel lymph nodes biopsy in 54 endometrial carcinoma patients. All 54 patients received systematic lymph node dissection after sentinel lymph node biopsy. The detection rate, sensitivity, specificity, and accuracy of systematic lymph node dissection and sentinel lymph node biopsy by CNPs were respectively analyzed. A nomogram model for predicting the success of sentinel lymph node mapping was established. Results The average number of lymph nodes removed in the CNP-labeled study group was higher than that in the control group (p
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- 2023
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8. Dose titration of osmotic release oral system methylphenidate in children and adolescents with attention-deficit hyperactivity disorder: a retrospective cohort study
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Youran Xu, Hsingwen Chung, Meng Shu, Yanfang Liu, Yongjing Zhang, and Hong Qiu
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Osmotic release oral system methylphenidate (OROS-MPH) ,Attention-deficit hyperactivity disorder (ADHD) ,Dose titration ,Real-world evidence ,Children and adolescents ,Pediatrics ,RJ1-570 - Abstract
Abstract Background Osmotic release oral system methylphenidate (OROS-MPH) is one of the most commonly used medication for attention-deficit hyperactivity disorder (ADHD), however, real-world knowledge on OROS-MPH dose titration has been limited. This study aims to summarize and visualise the OROS-MPH titration patterns in children and adolescents with ADHD in the United States (US) and Japan. Methods This retrospective cohort study used the US IBM® MarketScan® Commercial Claims and Encounters database from 2000 to 2019 and the Japan Medical Data Centre database from 2008 to 2019. New OROS-MPH users with ADHD were identified and split into child (6 to
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- 2023
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9. Purple sweet potato delphinidin-3-rutin represses glioma proliferation by inducing miR-20b-5p/Atg7-dependent cytostatic autophagy
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Meng Wang, Ke Liu, Huimin Bu, Hao Cong, Guokai Dong, Nana Xu, Changgen Li, Yunyun Zhao, Fei Jiang, Yongjing Zhang, Bo Yuan, Rongpeng Li, and Jihong Jiang
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glioma ,rutin ,autophagy ,miRNA-20b-5p ,Creb ,Atg7 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Glioma is the most common primary malignant intracranial tumor. Owing to highly aggressive invasiveness and metastatic properties, the prognosis of this disease remains poor even with surgery, radiotherapy, and chemotherapy. Rutin is a glycoside natural flavonoid that modulates microglia inflammatory profile and improves anti-glioma activity. Here, a glycoside flavonoid was extracted and named purple sweet potato delphinidin-3-rutin (PSPD3R). In an experiment using the subcutaneous xenograft model of human glioblastoma (GBM) and alamar blue assay, we found that PSPD3R suppressed the glioma proliferation both in vitro and in vivo. Flow cytometry assay and transmission electron microscopy observation revealed that PSPD3R stimulated glioma cell autophagy and apoptosis. High-throughput microRNA (miRNA) sequencing showed that PSPD3R substantially affected the miRNA expression of U251 cells. Acridine orange staining and immunoblotting indicated that PSPD3R regulated autophagy via Akt/Creb/miR-20b-5p in glioma cells. Luciferase reporter assays showed that autophagy-related gene 7 (Atg7) mRNA was the target gene of miR-20b-5p. The downregulation of miR-20b-5p inhibited glioma proliferation in vivo. In summary, PSPD3R regulated autophagy in glioma via the Akt/Creb/miR-20b-5p/Atg7 axis. This work unraveled the molecular mechanism of PSPD3R-induced autophagy in glioma and revealed its potential as a therapeutic agent for glioma treatment.
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- 2022
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10. Identification of immune related molecular subtypes and prognosis model for predicting prognosis, drug resistance in cervical squamous cell carcinoma
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Dongzhi Hu, Zijian Zhang, Yongjing Zhang, Kangni Huang, and Xiaoxue Li
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tumor microenvironment ,endocervical adenocarcinoma ,immunotherapy ,genomic mutations ,prognosis ,Genetics ,QH426-470 - Abstract
Background: One of the features of tumor immunity is the immunosuppressive tumor microenvironment (TME). In this study, TME gene signatures were used to define the characteristics of Cervical squamous cell carcinoma (CESC) immune subtypes and construct a new prognostic model.Methods: Single sample gene set enrichment analysis (ssGSEA) was used to quantify pathway activity. RNA-seq of 291 CESC were obtained from the Cancer Genome Atlas (TCGA) database as a training set. Microarray-based data of 400 cases of CESC were obtained from the Gene Expression Compilation (GEO) database as an independent validation set. 29 TME related gene signatures were consulted from previous study. Consensus Cluster Plus was employed to identify molecular subtype. Univariate cox regression analysis and random survival forest (RSF) were used to establish the immune-related gene risk model based on the TCGA data set of CESC, and the accuracy of prognosis prediction was verified by GEO data set. ESTIMATE algorithm was used to perform immune and matrix scores on the data set.Results: three molecular subtypes (C1, C2, C3) were screened in TCGA-CESC on account of 29 TME gene signatures. Among, C3 with better survival outcome had higher immune related gene signatures, while C1 with worse prognosis time had enhanced matrix related features. Increased immune infiltration, inhibition of tumor related pathways, widespread genomic mutations and prone immunotherapy were observed in C3. Furthermore, a five immune genes signature was constructed and predicted overall survival for CESC, which successfully validated in GSE44001 dataset. A positive phenomenon was observed between five hub genes expressions and methylation. Similarly, high group enriched in matrix related features, while immune related gene signatures were enriched in low group. Immune cell, immune checkpoints genes expression levels were negatively, while most TME gene signatures were positively correlated with Risk Score. In addition, high group was more sensitive to drug resistance.Conclusion: This work identified three distinct immune subtypes and a five genes signature for predicting prognosis in CESC patients, which provided a promising treatment strategy for CESC.
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- 2023
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11. The Mechanism of Transcription Factor Swi6 in Regulating Growth and Pathogenicity of Ceratocystis fimbriata: Insights from Non-Targeted Metabolomics
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Hao Cong, Changgen Li, Yiming Wang, Yongjing Zhang, Daifu Ma, Lianwei Li, and Jihong Jiang
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Ceratocystis fimbriata ,metabolomics ,Swi6 ,virulence ,sweet potato black rot ,Biology (General) ,QH301-705.5 - Abstract
Ceratocystis fimbriata (C. fimbriata) is a notorious pathogenic fungus that causes sweet potato black rot disease. The APSES transcription factor Swi6 in fungi is located downstream of the cell wall integrity (CWI)-mitogen-activated protein kinase (MAPK) signaling pathway and has been identified to be involved in cell wall integrity and virulence in several filamentous pathogenic fungi. However, the specific mechanisms by which Swi6 regulates the growth and pathogenicity of plant pathogenic fungi remain elusive. In this study, the SWI6 deletion mutants and complemented strains of C. fimbriata were generated. Deletion of Swi6 in C. fimbriata resulted in aberrant growth patterns. Pathogenicity assays on sweet potato storage roots revealed a significant decrease in virulence in the mutant. Non-targeted metabolomic analysis using LC-MS identified a total of 692 potential differentially accumulated metabolites (PDAMs) in the ∆Cfswi6 mutant compared to the wild type, and the results of KEGG enrichment analysis demonstrated significant enrichment of PDAMs within various metabolic pathways, including amino acid metabolism, lipid metabolism, nucleotide metabolism, GPI-anchored protein synthesis, and ABC transporter metabolism. These metabolic pathways were believed to play a crucial role in mediating the growth and pathogenicity of C. fimbriata through the regulation of CWI. Firstly, the deletion of the SWI6 gene led to abnormal amino acid and lipid metabolism, potentially exacerbating energy storage imbalance. Secondly, significant enrichment of metabolites related to GPI-anchored protein biosynthesis implied compromised cell wall integrity. Lastly, disruption of ABC transport protein metabolism may hinder intracellular transmembrane transport. Importantly, this study represents the first investigation into the potential regulatory mechanisms of SWI6 in plant filamentous pathogenic fungi from a metabolic perspective. The findings provide novel insights into the role of SWI6 in the growth and virulence of C. fimbriata, highlighting its potential as a target for controlling this pathogen.
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- 2023
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12. Efficacy of optical coherence tomography in the triage of women with minor abnormal cervical cytology before colposcopy.
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Lei Yan, Xiao Xiao, Ling He, Liye Shi, Xue Yang, Jun Zhang, Yongjing Zhang, and Chun Fu
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Medicine ,Science - Abstract
ObjectivesTo evaluate the efficacy of Optical Coherence Tomography (OCT) for detecting cervical lesions in women with minor abnormal cytology results (atypical squamous cells of undetermined significance (ASC-US) and low-grade squamous intraepithelial lesion (LSIL)).MethodsA prospective study was conducted at gynecologic clinic from Mar 2021 to Sep 2021. The recruited women with cervical cytological findings of ASC-US or LSIL were inspected with OCT before colposcopy-directed cervical biopsy. The diagnostic performance of OCT, alone and in combination with high-risk human papillomavirus (hrHPV) testing were evaluated to detect cervical intraepithelial neoplasia of grade 2 or worse (CIN2+)/CIN3 or worse (CIN3+). The rate of colposcopy referral and the immediate risk of CIN3+ of OCT were calculated.ResultsA total of 349 women with minor abnormal cervical cytology results were enrolled. For detection of CIN2+/CIN3+, the sensitivity and NPV of OCT were lower than those of hrHPV testing (CIN2+: 71.3% vs. 95.4%, 89.0% vs. 91.1%, P < 0.001; CIN3+: 75% vs. 93.8%, 96.5% vs. 95.6%, P < 0.001), but the specificity, accuracy and PPV were higher than those of hrHPV testing (CIN2+: 77.5% vs. 15.6%, 75.9% vs. 35.5%, 51.2% vs. 27.3%, P < 0.001; CIN3+: 69.4% vs. 13.6%, 69.9% vs. 20.9%, 19.8% vs. 9.9%, P < 0.001). OCT combined with hrHPV testing (CIN2+: 80.9%; CIN3+: 72.6%) showed higher specificity than that of OCT alone (P < 0.001). The colposcopy referral rate base on OCT classification was lower than that based on hrHPV testing (34.7% vs. 87.1%, P < 0.001). Patients with hrHPV-positive ASC-US and hrHPV-negative LSIL cytology, the immediate CIN3+ risk in OCT negative cases was less than 4%.ConclusionsOCT alone or combination with hrHPV testing shows good performance for detecting CIN2+/CIN3+ in patients with ASC-US/LSIL cytology. OCT is an effective method for colposcopy triage in women with hrHPV-positive ASC-US and hrHPV-negative LSIL cytology.
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- 2023
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13. Prognosis comparison between small cell carcinoma of ovary and high-grade serous ovarian cancer: A retrospective observational cohort study
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Dongzhi Hu, Dongdong Ma, Zi-jian Zhang, Yongjing Zhang, Kangni Huang, and Xiaoxue Li
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small cell carcinoma of ovary ,high-grade serous ovarian cancer ,overall survival ,cancer-specific survival ,SEER database ,Propensity score matching ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
BackgroundSmall cell carcinoma of ovary (SCCO) is a rare and aggressive cancer primarily reported in the form of case reports. Due to limited epidemiological and prognostic analyses based on large populations, SCCO has varied considerably without prognostic models and a recognized first-line treatment strategy. The study aimed to compare the clinical characteristics, treatment methods, and prognosis of SCCO and high-grade serous ovarian cancer (HGSOC), the most prevalent subtype of ovarian cancer, in a large sample and develop a predictive model for these two subtypes.MethodsData from the Surveillance, Epidemiology, and End Results program were analyzed for patients with SCCO or HGSOC from 2000 to 2017. Clinical, demographic, and treatment characteristics were compared between the two groups. Propensity-score matching, Cox risk regression analysis, and Kaplan-Meier survival curves were used to assess the data. Finally, a nomogram was developed to predict the patient survival time.ResultsA total of 32,185 women, including 31,979 (99.4%) diagnosed with HGSOC and 206 (0.6%) diagnosed with SCCO, were identified. Age ≤ 51 years, single, median house income less than $70,000, early stage, and unilateral disease were more common characteristics of patients with SCCO than those with HGSOC. Patients with SCCO were more likely to receive radiotherapy (6.8% vs. 0.8%, p
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- 2023
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14. Trends of sphincter-preserving surgeries for low lying rectal cancer: A 20-year experience in China
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Kuo Zheng, Qingqing Hu, Guanyu Yu, Leqi Zhou, Yuting Yao, Yuan Zhou, Hao Wang, Liqiang Hao, Enda Yu, Zheng Lou, Yongjing Zhang, Hong Qiu, Ronggui Meng, and Wei Zhang
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sphincter preservation ,low rectal cancer ,abdominoperineal resection ,trends in surgery ,low anterior resection ,rectal cancer survival ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
BackgroundOver the last 2 decades, patients with low rectal cancer have had better outcomes from improvements in surgical techniques in sphincter preservation. We aimed to quantify the trends in sphincter-preserving surgeries for low rectal cancer over 20 years in a top tertiary hospital in China.MethodsBetween 1999 and 2021, a cohort of patients with primary malignant rectal tumor ≤5cm from the anal verge and who received elective surgeries at Changhai Hospital, Shanghai, China, was identified. Data were extracted from electronic medical records. A Joinpoint Regression Model was used to analyze trends in surgical procedures by average annual percentage change (AAPC). Adjusted Cox proportional hazards regression model was used to assess overall survival.ResultsAmong a total of 4,172 patients during the study period, 3,111 (74.6%) underwent a sphincter-preserving surgery and 1,061 (25.4%) received APR. Sphincter-preserving surgery increased 3.6% per year (95%CI, 2.3-4.9). Low anterior resection was the most performed procedure (86.3%) and maintained a steady trend, while intersphincteric resection increased 49.4% annually (95%CI, 19.5-86.7) after initiation. Laparoscopic techniques increased 15.1% per year (95%CI, 8.4-43.4) after initiation. Sphincter-preserving surgery increased annually for tumors ≤2cm, 2-≤3cm and 3-≤4cm from the anal verge (AAPC 7.1, 4.5-9.8; 4.7, 3.1-6.3; 2.7, 1.7-3.6, respectively). Furthermore, patients with sphincter-preserving surgery had a better overall survival than abdominoperineal resection (APR) patients (adjusted HR 0.78, 95% CI, 0.65-0.93, p=.01).ConclusionsUtilization of sphincter-preserving surgeries increased significantly over the last 20 years. Patients with low rectal cancer who underwent sphincter preservation had better survival than similar patients who underwent APR.
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- 2022
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15. Cultivable Endophyte Resources in Medicinal Plants and Effects on Hosts
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Yiming Wang, Yongjing Zhang, Hao Cong, Changgen Li, Jiaying Wu, Ludan Li, Jihong Jiang, and Xiaoying Cao
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medicinal plants ,endophyte ,biocontrol ,medicinal components ,Science - Abstract
With the increasing demand for medicinal plants and the increasing shortage of resources, improving the quality and yield of medicinal plants and making more effective use of medicinal plants has become an urgent problem to be solved. During the growth of medicinal plants, various adversities can lead to nutrient loss and yield decline. Using traditional chemical pesticides to control the stress resistance of plants will cause serious pollution to the environment and even endanger human health. Therefore, it is necessary to find suitable pesticide substitutes from natural ingredients. As an important part of the microecology of medicinal plants, endophytes can promote the growth of medicinal plants, improve the stress tolerance of hosts, and promote the accumulation of active components of hosts. Endophytes have a more positive and direct impact on the host and can metabolize rich medicinal ingredients, so researchers pay attention to them. This paper reviews the research in the past five years, aiming to provide ideas for improving the quality of medicinal plants, developing more microbial resources, exploring more medicinal natural products, and providing help for the development of research on medicinal plants and endophytes.
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- 2023
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16. Machine Unlearning by Reversing the Continual Learning
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Yongjing Zhang, Zhaobo Lu, Feng Zhang, Hao Wang, and Shaojing Li
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machine unlearning ,continual learning ,elastic weight consolidation ,decreasing moment matching ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
Recent legislations, such as the European General Data Protection Regulation (GDPR), require user data holders to guarantee the individual’s right to be forgotten. This means that user data holders must completely delete user data upon request. However, in the field of machine learning, it is not possible to simply remove these data from the back-end database wherein the training dataset is stored, because the machine learning model still retains this data information. Retraining the model using a dataset with these data removed can overcome this problem; however, this can lead to expensive computational overheads. In order to remedy this shortcoming, we propose two effective methods to help model owners or data holders remove private data from a trained model. The first method uses an elastic weight consolidation (EWC) constraint term and a modified loss function to neutralize the data to be removed. The second method approximates the posterior distribution of the model as a Gaussian distribution, and the model after unlearning is computed by decreasingly matching the moment (DMM) of the posterior distribution of the neural network trained on all data and the data to be removed. Finally, we conducted experiments on three standard datasets using backdoor attacks as the evaluation metric. The results show that both methods are effective in removing backdoor triggers in deep learning models. Specifically, EWC can reduce the success rate of backdoor attacks to 0. IMM can ensure that the model prediction accuracy is higher than 80% and keep the success rate of backdoor attacks below 10%.
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- 2023
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17. Chimeric antigen receptor T cells self-neutralizing IL6 storm in patients with hematologic malignancy
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Lei Xue, Yan Yi, Qianwen Xu, Li Wang, Xiaohui Yang, Yongjing Zhang, Xuefei Hua, Xiaoshan Chai, Junjie Yang, Yaxin Chen, Guangshi Tao, Biliang Hu, and Xingbing Wang
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Cytology ,QH573-671 - Abstract
Abstract IL6 is one of the most elevated cytokines during chimeric antigen receptor (CAR) T cell cytokine release syndrome (CRS), and IL6R blockade by Tocilizumab has successfully relieved the most life-threatening aspects of CRS in patients. In addition, latest studies demonstrated the essential role of IL1 in driving CART induced neurotoxicity in mouse models. Here we present a clinical investigation (ChiCTR2000032124; ChiCTR2000031868) of anti-CD19 and anti-BCMA CART (41BBζ) secreting an anti-IL6 scFv and IL1 receptor antagonist (IL1RA) in treating patients with hematologic malignancy. Our results revealed that IL6 and IL1B were maintained at low levels without significant elevation during CRS, rendering Tocilizumab dispensable. Moreover, treated patients did not show neurotoxicity during CRS and exhibited mild to moderate CRS. Notably, we observed high rate of complete response (CR) and significant CART expansion during treatment. In sum, we conclude that CART-secreting anti-IL6 scFv and IL1RA could self-neutralize IL6 storm and maintain low levels of IL1B during CART therapy to minimize IL6- and IL1-associated cytokine toxicity and neurotoxicity without impairing therapeutic efficacy.
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- 2021
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18. Editorial: China and the world–Cultural differences, transmissions and transitions
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Marc Oliver Rieger, Mei Wang, and Yongjing Zhang
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China ,culture ,belt and road initiative (BRI) ,patriotism and nationalism ,COVID-19 ,intellectual property rights (IPR) ,Sociology (General) ,HM401-1281 - Published
- 2022
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19. Concordance of the treatment patterns for major depressive disorders between the Canadian Network for Mood and Anxiety Treatments (CANMAT) algorithm and real-world practice in China
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Lu Yang, Yousong Su, Sijia Dong, Tao Wu, Yongjing Zhang, Hong Qiu, Wenjie Gu, Yifeng Xu, JianLi Wang, Jun Chen, and Yiru Fang
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depressive disorder ,treatment patterns ,guideline adherence ,treatment algorithm ,real-world studies ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Background: Antidepressant (AD) algorithm is an important tool to support treatment decision-making and improve management of major depressive disorder (MDD). However, little is known about its concordance with real-world practice. This study aimed to assess the concordance between the longitudinal treatment patterns and AD algorithm recommended by a clinical practice guideline in China.Methods: Data were obtained from the electronic medical records of Shanghai Mental Health Center (SMHC), one of the largest mental health institutions in China. We examined the concordance between clinical practice and the Canadian Network for Mood and Anxiety Treatments (CANMAT) algorithm among a cohort composed of 19,955 MDD patients. The longitudinal characteristics of treatment regimen and duration were described to identify the specific inconsistencies. Demographics and health utilizations of the algorithm-concordant and -discordant subgroups with optimized treatment were measured separately.Results: The overall proportion of algorithm-concordant treatment significantly increased from 84.45% to 86.03% during the year of 2015–2017. Among the patients who received recommended first-line drugs with subsequent optimized treatment (n = 2977), the concordance proportion was 27.24%. Mirtazapine and trazodone were the most used drugs for adjunctive strategy. Inadequate or extended duration before optimized treatment are common inconsistency. The median length of follow-up for algorithm-concordant (n = 811) and algorithm-discordant patients (n = 2166) were 153 days (Q1-Q3 = 79–328) and 368 days (Q1-Q3 = 181–577) respectively, and the average number of clinical visits per person-year was 13.07 and 13.08 respectively.Conclusion: Gap existed between clinical practice and AD algorithm. Improved access to evidence-based treatment is required, especially for optimized strategies during outpatient follow-up.
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- 2022
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20. CRISPR-edited CART with GM-CSF knockout and auto secretion of IL6 and IL1 blockers in patients with hematologic malignancy
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Yan Yi, Xiaoshan Chai, Liping Zheng, Yongjing Zhang, Jiankai Shen, Biliang Hu, and Guangshi Tao
- Subjects
Cytology ,QH573-671 - Abstract
Abstract Revolutionary CART therapy still faces the challenge of severe cytokine release syndrome (CRS). While IL6 and IL1 have been demonstrated as essential contributors, GM-CSF is one of the most abundant inflammatory cytokines secreted by CART and has also been suggested in contributing to CRS. To minimize GM-CSF production from CART to reduce its associated toxicity, we conducted a pilot study (ChiCTR2000032124) of CRISPR-edited GM-CSF knockout (KO) in CART secreting anti-IL6 scFv and IL1RA, with additional TCR KO for tracing edited CART. The initial results of three patients (1 Non-Hodgkin lymphoma (NHL) and 2 multiple myelomas (MMs)) are summarized as: 3/3 complete response, 2/3 none CRS, 1/3 grade 2 CRS, and 0/3 neurotoxicity. The analysis revealed low levels of GM-CSF, IL6 and IL1B at the time of interferon-gamma (IFNG) peaks, and elevated IL1RA. We also observed significant expansion of CD3– CART during treatment and no aberrant expansion of CD3– CART in the follow-up. Re-expansion of CD3– CART was observed in two patients while recurring CD19+ cells were eradicated in the patient with NHL. In summary, our study supported the safety and durable potency of CRISPR-edited CART in patients, providing a novel platform for developing autologous or allogeneic CART to minimize GM-CSF-associated toxicity in addition to autonomous IL6/IL1 blockade.
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- 2021
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21. Hypoxia Enhances HIF1α Transcription Activity by Upregulating KDM4A and Mediating H3K9me3, Thus Inducing Ferroptosis Resistance in Cervical Cancer Cells
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Jing Xiong, Meifang Nie, Chun Fu, Xiaoshan Chai, Yongjing Zhang, Ling He, and Shujuan Sun
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Internal medicine ,RC31-1245 - Abstract
Objective. Cervical cancer (CC) is a prevalent cancer in women. Hypoxia plays a critical role in CC cell ferroptosis resistance. This study explored the mechanism of hypoxia in CC cell ferroptosis resistance by regulating HIF1α/KDM4A/H3K9me3. Methods. Cultured SiHa and Hela cells were exposed to CoCl2 and treated with Erastin. Cell viability was detected by MTT assay, and concentrations of iron ion, MDA and GSH were determined using corresponding kits. Expressions of KDM4A, HIF1α, TfR1, DMT1, and H3k9me3 were detected by RT-qPCR, Western blot, and ChIP assay. The correlation of KDM4A and HIF1α was analyzed on Oncomine, UALCAN, and Starbase. CC cells were co-transfected with shKDM4A or/and pcDNA3.1-HIF1α. Iron uptake and release were assessed using the isotopic tracer method. The binding relationship between HIF1α and HRE sequence was verified by dual-luciferase assay. Results. Cell viability and GSH were decreased while iron concentration, MDA, KDM4A, and HIF1α levels were increased in hypoxia conditions. The 2-h hypoxia induced ferroptosis resistance. KDM4A and HIF1α were highly-expressed in CC tissues and positively correlated with each other. KDM4A knockdown attenuated cell resistance to Erastin, increased H3K9me3 level in the HIF1α promoter region, and downregulated HIF1α transcription and translation. H3K9me3 level was increased in the HIF1α promoter after hypoxia. HIF1α overexpression abrogated the function of KDM4A knockdown on ferroptosis in hypoxia conditions. Iron uptake/release and TfR1/DMT1 levels were increased after hypoxia. Hypoxia activated HRE sequence in TfR1 and DMT1 promoters. Conclusion. Hypoxia upregulated KDM4A, enhanced HIF1α transcription, and activated HRE sequence in TfR1 and DMT1 promoters via H3K9me3, thus inducing ferroptosis resistance in CC cells.
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- 2022
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22. Major vault protein suppresses obesity and atherosclerosis through inhibiting IKK–NF-κB signaling mediated inflammation
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Jingjing Ben, Bin Jiang, Dongdong Wang, Qingling Liu, Yongjing Zhang, Yu Qi, Xing Tong, Lili Chen, Xianzhong Liu, Yan Zhang, Xudong Zhu, Xiaoyu Li, Hanwen Zhang, Hui Bai, Qing Yang, Junqing Ma, Erik A. C. Wiemer, Yong Xu, and Qi Chen
- Subjects
Science - Abstract
Metabolic diseases are associated with chronic, low-grade inflammation. Here the authors show that major vault protein (MVP) suppresses NF-κB signalling in macrophages via an IRAK1–TRAF6 axis and that loss of MVP in myeloid cells exacerbates the inflammatory response in mice fed a high fat diet.
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- 2019
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23. ALT Flare Predicts Hepatocellular Carcinoma Among Antiviral Treated Patients With Chronic Hepatitis B: A Cross-Country Cohort Study
- Author
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Yanan Du, Bingying Du, Xiaoyan Fang, Meng Shu, Yongjing Zhang, Hsingwen Chung, Ye Sun, Jiaming Teng, Phimphone Visalath, Hong Qiu, and Wei Cai
- Subjects
alanine aminotransferase flare ,hepatocellular carcinoma ,risk factor ,chronic hepatitis B ,nucleos(t)ide analog ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
ObjectivesAlanine aminotransferase (ALT) level is one of the crucial indexes to evaluate disease status for chronic hepatitis B (CHB) patients. However, whether the ALT level after nucleos(t)ide analog (NA) treatment is associated with hepatocellular carcinoma (HCC) development remains unclear.Materials and MethodsWe evaluated the association between ALT level and HCC occurrence in NA-treated patients and investigated the predictive value of ALT flare for HCC. The associations between ALT level and HCC were analyzed by logistic regression and Cox proportional hazards models.ResultsThere were 21,223 CHB patients at Ruijin Hospital of China and 16,737 CHB patients in the Optum electronic health records (EHR) in the United States (US) treated with NAs between 2010 and 2018. Among them, 8,152 and 4,893 patients who achieved a normal ALT value were included in the study cohorts, respectively. A significant positive dose-dependent correlation between the peak ALT level and HCC was identified in both cohorts. Within the China cohort, ALT flare was significantly associated with increased risks of HCC compared to normal ALT (HR 2.55, 95%CI 1.45–4.50). Stronger increased risks associated with ALT flare were observed in the US cohort (HR 7.62, 95%CI 4.85–11.98).ConclusionsALT flare is a strong predictor for HCC occurrence in the CHB patients treated with NAs. Elevation of ALT, especially ALT flare warrants close monitoring for early HCC detection.
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- 2021
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24. Differences in the oral and intestinal microbiotas in pregnant women varying in periodontitis and gestational diabetes mellitus conditions
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Xin Zhang, Pei Wang, Liangkun Ma, Rongjun Guo, Yongjing Zhang, Peng Wang, Jizhi Zhao, and Juntao Liu
- Subjects
periodontitis ,gestational diabetes mellitus ,oral microbiota ,intestinal microbiota ,pregnant ,Infectious and parasitic diseases ,RC109-216 ,Microbiology ,QR1-502 - Abstract
Background: The present study aimed to investigate the potential association between oral and intestinal microbiotas of pregnant women with periodontitis and/or gestational diabetes mellitus (GDM) in the second trimester.Methods: Four groups were defined: periodontitis (n = 28), GDM (n = 7), periodontitis + GDM (n = 7), and periodontitis- and GDM-free controls (n = 27). The oral and intestinal microbiomes were analyzed using the 16S rRNA sequencing technique.Results: Periodontitis alone significantly decreased the oral microbial diversity (by Shannon index, p = 0.003) and changed the structure of the oral microbial community (by AMOVA, p 0.001). GDM alone significantly increased the oral microbial diversity (by Shannon index, p = 0.049), and when combined with periodontitis, GDM significantly decreased the intestinal microbial richness (by observed species, p = 0.018) and influenced the structure of intestinal microbial community (by AMOVA, p = 0.043). The differentially abundant microbial taxa among different groups in both oral and intestinal samples were identified by LEfSe analysis, and limited taxa showed consistent trends. The numbers and ratios of oral-intestinal shared operational taxonomical units were the least in the periodontitis + GDM group.Conclusions: A close relationship between the oral microbiota and pregnant periodontitis was shown. Significant changes occur in both the oral and intestinal microbiomes when periodontitis was coupled with GDM. A separate influence of periodontitis and GDM on the oral and intestinal microbiotas may be indicated.
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- 2021
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25. IoT-based generalized multi-granulation sequential three-way decisions.
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Yongjing Zhang, Guannan Li, Wangchen Dai, Chengxin Hong, Jin Qian, and Zhaoyang Han
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- 2023
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26. LAFED: A lightweight authentication mechanism for blockchain-enabled federated learning system.
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Shan Ji, Jiale Zhang, Yongjing Zhang, Zhaoyang Han, and Chuan Ma
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- 2023
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27. Unified Attribute-Based Encryption Scheme for Industrial Internet of Things.
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Wei Li, Jing Si, Jianhua Xing, Yongjing Zhang, Deli Liu, and Zhiyuan Sui
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- 2021
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28. Research on Malware Variant Detection Method Based on Deep Neural Network.
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Jianhua Xing, Jing Si, Yongjing Zhang, Wei Li, and Yuning Zheng
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- 2021
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29. Secreted phosphoprotein 1 regulates natural compound 3',4',5,7-tetrahydroxyflavone to inhibit mast cell-mediated allergic inflammation.
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Shiling Hu, Jue Wang, Haoyun Bai, Chaohua Feng, Zhenqi Zhou, Zhuoyin Xue, Wen Zhang, Yongjing Zhang, Nan Wang, and Langchong He
- Subjects
TRYPTASE ,MAST cells ,CULTIVARS ,CALCIUM ions ,HISTAMINE ,INFLAMMATION ,KOUNIS syndrome - Abstract
Background: Mast cells (MCs) are important effector cells in anaphylaxis and anaphylactic disease. 3',4',5,7-tetrahydroxyflavone (THF) presents in many medicinal plants and exerts a variety of pharmacological effects. In this study, we evaluated the effect of THF on C48/80-induced anaphylaxis and the mechanisms underlying its effects, including the role of secreted phosphoprotein 1 (SPP1), which has not been reported to IgE-independent MC activation. Results: THF inhibited C48/80-induced Ca2+ flow and degranulation via the PLC/PKC/IP3 pathway in vitro. RNA-seq showed that THF inhibited the expression of SPP1 and downstream molecules. SPP1 is involved in pseudo-anaphylaxis reactions. Silencing SPP1 affects the phosphorylation of AKT and P38. THF suppressed C48/80-induced paw edema, hypothermia and serum histamine, and chemokines release in vivo. Conclusions: Our results validated SPP1 is involved in IgE-independent MC activation anaphylactoid reactions. THF inhibited C48/80-mediated anaphylactoid reactions both in vivo and in vitro, suppressed calcium mobilization and inhibited SPP1-related pathways. [ABSTRACT FROM AUTHOR]
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- 2023
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30. Substance P‐induced lung inflammation in mice is mast cell dependent
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Nan Wang, Jue Wang, Tao Zhang, Shuanying Yang, Yongjing Zhang, Langchong He, Shiling Hu, Xiuzhen Sun, Tianxiao Zhang, and Yuanyuan Wu
- Subjects
Immunology ,Inflammation ,Substance P ,Immunoglobulin E ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Fibrosis ,Animals ,Humans ,Immunology and Allergy ,Medicine ,Mast Cells ,Lung ,Asthma ,Neurogenic inflammation ,medicine.diagnostic_test ,biology ,business.industry ,Pneumonia ,Mast cell ,medicine.disease ,respiratory tract diseases ,Mice, Inbred C57BL ,Disease Models, Animal ,Bronchoalveolar lavage ,medicine.anatomical_structure ,030228 respiratory system ,biology.protein ,Cytokines ,medicine.symptom ,business ,Bronchoalveolar Lavage Fluid ,030215 immunology - Abstract
BACKGROUND Allergic asthma is a common inflammatory lung disease and a major health problem worldwide. Mast cells (MCs) play a key role in the early-stage pathophysiology of allergic asthma. Substance P (SP) functions in neurogenic inflammation by activating MCs, and therefore, it may to participate in the occurrence and development of asthma. OBJECTIVE We examined the relationship between SP and lung inflammation, and also whether SP can directly trigger asthma. METHODS We measured the number of peripheral blood eosinophils, neutrophils and basophils and evaluated the levels of IgE and SP in blood samples of 86 individuals with allergic asthma. Serum IgE and SP levels were also determined in 29 healthy individuals. C57BL/6 mice were subjected to different doses of SP, and bronchoalveolar lavage fluid (BALF) was collected to count the inflammatory cells. Lung tissues were analysed using histopathological methods to evaluate lung peribronchial inflammation, fibrosis and glycogen deposition. Levels of IgE, interleukin (IL)-1, IL-2, IL-4, IL-5, IL-13, IL-17 and IFN-γ were determined in mouse serum. RESULTS Substance P levels were increased in the serum samples of patients with asthma. SP induced mouse lung peribronchial inflammation, fibrosis and glycogen deposition, with high levels of Th2-related cytokines such as IL-4, IL-5 and IL-13 observed in the BALF. Furthermore, low level of total IgE was noted in the serum, and SP had little effect on MC-deficient kitW-sh/W-sh mice. CONCLUSIONS & CLINICAL RELEVANCE Substance P levels increased significantly in serum of asthmatic patients and independently associated with the risk of asthma. Furthermore, SP induced Th2 lung inflammation in mice, which was dependent on MCs.
- Published
- 2021
31. Chrysin Inhibits Pseudo-allergic Reaction by Suppressing Mitochondrial STAT3 Activation via MAS-Related GPR Family Member X2
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Nan Wang, Yongjing Zhang, Zhuoyin Xue, Jue Wang, Shiling Hu, and Haoyun Bai
- Subjects
0106 biological sciences ,MAPK/ERK pathway ,biology ,010401 analytical chemistry ,chemistry.chemical_element ,General Chemistry ,Calcium ,Pharmacology ,01 natural sciences ,Adenosine ,0104 chemical sciences ,Serine ,chemistry.chemical_compound ,chemistry ,In vivo ,medicine ,biology.protein ,Glycolysis ,Chrysin ,General Agricultural and Biological Sciences ,STAT3 ,010606 plant biology & botany ,medicine.drug - Abstract
Chrysin, one of the most pharmacologically active natural flavonoids, has been extracted from various plants. Mast cells are an important part of innate immunity-mediating anaphylaxis. Pseudo-allergic reactions are currently believed to be associated with the MAS-related GPR family member X2 (MrgX2). In this study, the anti-pseudo allergy effect of chrysin and its underlying mechanisms were studied in vitro and in vivo. Chrysin inhibited passive cutaneous anaphylaxis and systemic pseudo-allergy in vivo. LAD2 cell degranulation, calcium ion (Ca2+) influx, and adenosine 5'-triphosphate (ATP) content were significantly suppressed in a dose-dependent manner. Chrysin suppressed pseudo-allergic reactions through the PLC/IP3/Ca2+ and ERK/STAT3 serine 727 pathways downstream of MrgX2. Therefore, mitochondrial ATP, but not glycolysis, is vital for pseudo-allergic reactions mediated by MrgX2. This study provides new insights for the treatment of pseudo-allergy.
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- 2021
32. Harringtonine: A more effective antagonist for Omicron variant
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Shiling Hu, Nan Wang, Shaohong Chen, Huajun Zhang, Cheng Wang, Weina Ma, Xinghai Zhang, Yan Wu, Yanni Lv, Zhuoyin Xue, Haoyun Bai, Shuai Ge, Huaizhen He, Wen Lu, Tao Zhang, Yuanyuan Ding, Rui Liu, Shengli Han, Yingzhuan Zhan, Guanqun Zhan, Zengjun Guo, Yongjing Zhang, Jiayu Lu, Jiapan Gao, Qianqian Jia, Yuejin Wang, Hongliang Wang, Shemin Lu, Tengchuan Jin, Sandra Chiu, and Langchong He
- Subjects
Pharmacology ,Biochemistry - Published
- 2023
33. Biomimetic design of gradient and hierarchical platform based on carbon nanotubes and porous carbon polyhedrons for strain sensors and beyond
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Songfang Zhao, Lin Liu, Yikun Liu, Yunlong Zhou, Meili Xia, Yongjing Zhang, Yang Li, Duxia Cao, Yongju Gao, and Yuekun Lai
- Subjects
General Engineering ,Ceramics and Composites - Published
- 2023
34. Multi-access radio resource management using multi-agent system.
- Author
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Zhiyong Feng 0001, Kai Yu, Yang Ji 0001, Ping Zhang 0003, Victor O. K. Li, and Yongjing Zhang
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- 2006
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35. How China Escaped Shock Therapy: The Market Reform Debate By Isabella M. Weber. New York: Routledge Press, 2021. xxx, 342 pp. ISBN: 9781138592193 (cloth)
- Author
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Yongjing Zhang
- Subjects
Cultural Studies ,History - Published
- 2022
36. α-Asarone alleviates allergic asthma by stabilizing mast cells through inhibition of ERK/JAK2-STAT3 pathway
- Author
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Haoyun Bai, Zhuoyin Xue, Wen Zhang, Chaohua Feng, Zhenqi Zhou, Shiling Hu, Yongjing Zhang, Qiaohong Qin, Yuanyuan Wu, Xiuzhen Sun, Yuhan Zhou, and Nan Wang
- Subjects
Clinical Biochemistry ,Molecular Medicine ,General Medicine ,Biochemistry - Abstract
Asthma is a heterogeneous disease related to numerous inflammatory cells, among which mast cells play an important role in the early stages of asthma. Therefore, treatment of asthma targeting mast cells is of great research value. α-Asarone is an important anti-inflammatory component of the traditional Chinese medicine Acorus calamus L, which has a variety of medicinal values. To investigate whether α-asarone can alleviate asthma symptoms and its mechanism. In this study, we investigated the effect of α-asarone on mast cell activation in vivo and in vitro. The release of chemokines or cytokines, AHR (airway hyperresponsiveness), and mast cell activation were examined in a mast cell-dependent asthma model. Western blot was performed to determine the underlying pathway. α-Asarone inhibited the degranulation of LAD2 (laboratory allergic disease 2) cells and decreased IL-8, MCP-1, histamine, and TNF-α in vitro. α-Asarone reduced paw swelling and leakage of Evans blue, as well as serum histamine, CCL2, and TNF-α in vivo. In the asthma model, α-asarone showed an inhibitory effect on AHR, inflammation, mast cells activation, infiltration of inflammatory cells, and the release of IL-5 and IL-13 in lung tissue. α-Asarone decreased the levels of phosphorylated JAK2, phosphorylated ERK, and phosphorylated STAT3 induced by C48/80. Our findings suggest that α-asarone alleviates allergic asthma by inhibiting mast cell activation through the ERK/JAK2-STAT3 pathway.
- Published
- 2022
37. MUSE: a vision of service and architecture for beyond 3G networks.
- Author
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Ping Zhang 0003, Yang Ji 0001, Yongjing Zhang, Xu Wang, and Zheng Hu 0001
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- 2005
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38. Dexamethasone inhibits SARS-CoV-2 spike pseudotyped virus viropexis by binding to ACE2
- Author
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Shiling Hu, Nan Wang, Yongjing Zhang, Zhuoyin Xue, Jue Wang, and Cheng Wang
- Subjects
endocrine system ,Cell ,ACE2 ,Biology ,Article ,Dexamethasone ,Virus ,Cell membrane ,03 medical and health sciences ,Virology ,polycyclic compounds ,medicine ,Humans ,Viability assay ,Binding site ,030304 developmental biology ,0303 health sciences ,Binding Sites ,SARS-CoV-2 ,030302 biochemistry & molecular biology ,HEK 293 cells ,Virus Internalization ,Viropexis ,Molecular biology ,Molecular Docking Simulation ,HEK293 Cells ,medicine.anatomical_structure ,Spike Glycoprotein, Coronavirus ,Angiotensin-converting enzyme 2 ,Angiotensin-Converting Enzyme 2 ,hormones, hormone substitutes, and hormone antagonists ,Protein Binding - Abstract
The SARS-CoV-2 outbreak, began in late 2019, has caused a worldwide pandemic and shows no signs of slowing. Glucocorticoids (GCs), including dexamethasone (DEX), have been widely used as effective anti-inflammatory and immunosuppressant drugs. In this study, seven GCs had no obvious effect on cell viability of angiotensin converting enzyme 2 (ACE2) high expressed HEK293T cells when concentrations were under 10 μM. Molecular docking results revealed that DEX occupied with active binding site of ACE2 of SARS-CoV-2 spike protein. Surface plasmon resonance (SPR) results showed that KD value between DEX and ACE2 was (9.03 ± 0.78) e−6 M. Cell membrane chromatography (CMC) results uncovered that DEX had a chromatographic retention. DEX was found out to inhibiting the viropexis into ACE2h cells using SARS-CoV-2 spike pseudotyped virus. Therefore, DEX inhibits the entrance of SARS-CoV-2 spike pseudotyped virus into cell by binding to ACE2., Highlights •DEX inhibits the entrance of 2019-nCoV spike pseudotyped virus into cells. •DEX can bind to ACE2. •The binding sites of DEX to two proteins interacts with each other when virus entry according to molecular docking.
- Published
- 2021
39. Harringtonine has the effects of double blocking SARS-CoV-2 membrane fusion
- Author
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Shiling Hu, Nan Wang, Shaohong Chen, Qiang Ding, Cheng Wang, Weina Ma, Xinghai Zhang, Yan Wu, Yanni Lv, Zhuoyin Xue, Haoyun Bai, Shuai Ge, Huaizhen He, Wen Lu, Tao Zhang, Yuanyuan Ding, Rui Liu, Shengli Han, Yingzhuan Zhan, Guanqun Zhan, Zengjun Guo, Yongjing Zhang, Jiayu Lu, Jiapan Gao, Qianqian Jia, Yuejin Wang, Hongliang Wang, Shemin Lu, Huajun Zhang, and Langchong He
- Subjects
body regions ,viruses ,fungi ,skin and connective tissue diseases - Abstract
Fusion with host cell membrane is the main mechanism of infection of SARS-CoV-2. Here, we propose a new strategy to double block SARS-CoV-2 membrane fusion by using Harringtonine (HT), a small-molecule antagonist. By using cell membrane chromatography (CMC), we found that HT specifically targeted the SARS-CoV-2 S protein and host cell TMPRSS2, and then confirmed that HT can inhibit pseudotyped virus membrane fusion. Furthermore, HT successfully blocked SARS-CoV-2 infection, especially in the delta and Omicron mutant. Since HT is a small-molecule antagonist, it is minimally affected by the continuous variation of SARS-CoV-2. Our findings show that HT is a potential small-molecule antagonist with a new mechanism of action against SARS-CoV-2 infection, and thus HT mainly targets the S protein, and thus, greatly reduces the damage of the S protein’s autotoxicity to the organ system, has promising advantages in the clinical treatment of COVID-19.
- Published
- 2022
40. Persistence With and Adherence to Paliperidone Palmitate Once-Monthly Injection for Schizophrenia Treatment in China and Japan
- Author
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Huaning Wang, Yongjing Zhang, Jin Liu, Rui Chi, Tao Wu, Ting Zhang, Lili Zhang, Kun Jiang, Hong Qiu, Wentian Dong, and Tianmei Si
- Subjects
Adult ,Male ,China ,Adolescent ,Administration, Oral ,Middle Aged ,Injections ,Medication Adherence ,Young Adult ,Psychiatry and Mental health ,Japan ,Delayed-Action Preparations ,Paliperidone Palmitate ,Schizophrenia ,Humans ,Female ,Antipsychotic Agents ,Retrospective Studies - Published
- 2021
41. Energy storage performance in polymer dielectrics by introducing 2D SrBi4Ti4O15 nanosheets
- Author
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Ying Lin, Shili Zhan, Yongjing Zhang, Qibin Yuan, Haibo Yang, and Chuang Sun
- Subjects
Materials science ,02 engineering and technology ,Dielectric ,01 natural sciences ,Energy storage ,law.invention ,law ,Electric field ,0103 physical sciences ,Materials Chemistry ,Molten salt ,010302 applied physics ,chemistry.chemical_classification ,Nanocomposite ,business.industry ,Process Chemistry and Technology ,Polymer ,021001 nanoscience & nanotechnology ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Capacitor ,Film capacitor ,chemistry ,Ceramics and Composites ,Optoelectronics ,0210 nano-technology ,business - Abstract
Polymer-based dielectric energy storage materials have been playing critical roles in high-performance energy storage capacitors due to their high breakdown electric fields and facile processability. However, the low energy density and efficiency of polymer-based film capacitors limit their widespread application in various electronics products. In this contribution, we synthesized the SrBi4Ti4O15 (SBT) nanosheets with large aspect ratio by using the molten salt method and introduced them into the PVDF matrix to prepare a multilayer nanocomposite film, of which the discharge energy density and discharge efficiency were much improved. Finite element simulation results show that the addition of 2D SBT nanosheets can significantly improve the local electric field distribution of nanocomposite films. Finally, a high discharge energy density of 11.69 J cm−3 and an excellent discharge efficiency of 78.95% were simultaneously achieved in the 0/5/0 sample. This work provides a reference for polymer-based dielectrics with excellent comprehensive energy storage performance.
- Published
- 2020
42. Isoimperatorin reduces the effective dose of dexamethasone in a murine model of asthma by inhibiting mast cell activation
- Author
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Xiuzhen Sun, Shuai Ge, Nan Wang, Yingnan Zeng, Min Jia, Yuanyuan Wu, Yongjing Zhang, and Jue Wang
- Subjects
Inflammation ,Pharmacology ,Dexamethasone ,Mice ,03 medical and health sciences ,0302 clinical medicine ,In vivo ,Furocoumarins ,medicine ,Animals ,Humans ,Mast Cells ,0303 health sciences ,Chemistry ,Furocoumarin ,030302 biochemistry & molecular biology ,Degranulation ,Mast cell ,Effective dose (pharmacology) ,Asthma ,Disease Models, Animal ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Tumor necrosis factor alpha ,medicine.symptom ,medicine.drug - Abstract
Adverse effects that result from dexamethasone (DEX) use are common and serious in patients with asthma. Therefore, alternative anti-inflammatory treatments are being investigated. Isoimperatorin (ISO), an active natural furocoumarin, possesses multiple pharmacological properties, including an anti-inflammation effect. In this study, investigations were conducted on the effect of ISO on mast cell (MC) activation in vitro and whether ISO could reduce the effective dose of DEX in a mast cell-dependent murine model of asthma in vivo. Calcium imaging was used to assess intracellular Ca2+ mobilization. Enzyme-linked immunosorbent assay was used to measure the chemokines release. Western blot analysis was conducted to investigate the underlying pathway. Airway inflammation and hyperresponsiveness (AHR) were examined in an asthma model. ISO inhibited Ca2+ flux and MC degranulation via Lyn/PLCγ1/PKC, ERK, and P38 MAPK pathways. In the asthma model, ISO, in combination with DEX, showed an additive inhibitory effect on AHR, inflammation, and the number of activated MCs in the lungs and decreased the levels of interleukin (IL)-4, IL-5, IL-6, IL-13, tumor necrosis factor (TNF)-a, and C-C motif chemokine ligand (CCL)-2 in bronchoalveolar lavage fluid. A combination of DEX and ISO may be appropriate if a decrease in the steroid dose is desired owing to dose-dependent adverse effects.
- Published
- 2020
43. Multilayer Structured Poly(vinylidene fluoride)-Based Composite Film with Ultrahigh Breakdown Strength and Discharged Energy Density
- Author
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Zhener Dang, Chuang Sun, Qibin Yuan, Shili Zhan, Haibo Yang, Guangliang Hu, Ying Lin, and Yongjing Zhang
- Subjects
chemistry.chemical_classification ,Materials science ,Doping ,Composite number ,02 engineering and technology ,Dielectric ,Polymer ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Energy storage ,0104 chemical sciences ,chemistry ,visual_art ,Electric field ,visual_art.visual_art_medium ,General Materials Science ,Ceramic ,Composite material ,0210 nano-technology ,Voltage - Abstract
Poly(vinylidene fluoride)-based (PVDF) composites with high discharged energy density (Ue) have been considered as advanced dielectric materials for pulsed power systems and electrical weapon systems. However, further improvement of the Ue of PVDF-based composites with higher breakdown strength (Eb) is of utmost importance. Based on the principle of voltage distribution in the multilayer structure, the multilayer structure (five-layer structure) ceramic/polymer composite films consisting of pristine PVDF layers with high breakdown strength and PVDF layers doped with one-dimensional 0.15SrTiO3-0.85Na0.5Bi0.5TiO3 (1D SNBT) fibers where the two kinds of layers stacked alternately has been designed and fabricated. Accordingly, a series of analyses of the dielectric properties and energy storage performances are presented, and the related results are tentatively explained by finite element simulation. The energy storage characteristics of the composite films are greatly improved due to the newly designed structure. An excellent Ue of 20.82 J cm-3 is achieved at an ultrahigh electric field of 640 MV m-1 with the PVDF layers containing 6 vol % SNBT fibers. Therefore, this work provides a new strategy to design and fabricate advanced polymer-based energy storage materials.
- Published
- 2020
44. Risk of Serious Ventricular Arrhythmia in Users of Gastrointestinal Medications: A Retrospective Cohort Study in China
- Author
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Kun Jiang, Kaichun Wu, Yongjing Zhang, Dianxin Zhang, Wenhua Liang, Meng Shu, and Hong Qiu
- Subjects
Adult ,Male ,China ,030213 general clinical medicine ,medicine.medical_specialty ,Databases, Factual ,Gastrointestinal Diseases ,medicine.drug_class ,Administration, Oral ,Proton-pump inhibitor ,Sudden death ,03 medical and health sciences ,0302 clinical medicine ,Gastrointestinal Agents ,Internal medicine ,medicine ,Humans ,Pharmacology (medical) ,Proportional Hazards Models ,Retrospective Studies ,business.industry ,Incidence (epidemiology) ,Hazard ratio ,Arrhythmias, Cardiac ,Proton Pump Inhibitors ,Retrospective cohort study ,General Medicine ,Middle Aged ,Itopride ,Mosapride ,Domperidone ,030220 oncology & carcinogenesis ,Antiemetics ,Dopamine Antagonists ,Female ,business ,medicine.drug - Abstract
Clinical data have demonstrated prolongation of the QTc interval associated with domperidone use. The risk of serious ventricular arrhythmias (SVA) in clinical practice in China has not been investigated. The incidence of SVA and all-cause cardiac arrest associated with medications for gastrointestinal disorders in China was assessed in a retrospective cohort study using information from electronic medical record databases from the Xijing and Guangzhou general hospitals. Inpatients and outpatients with at least one prescription for domperidone, mosapride/itopride, metoclopramide, a proton pump inhibitor (PPI), or histamine H2 receptor antagonist were identified in the hospital databases from January 1, 2009 to December 31, 2014. The inpatient exposure cohorts included 66,282 inpatients at Xijing and 23,643 inpatients at Guangzhou hospitals. There were 67 cases of SVA and two cases of SVA at the respective hospitals during the study period. Three cases (all at Xijing) occurred in patients prescribed domperidone (incidence rate 2.9/100 person-years (PYs), 95% CI 0.9–9.0) compared to 1.3/100 PYs (95% CI 0.2–8.9) for mosapride/itopride and 5.6/100 PYs (95% CI 4.4–7.2) for PPIs. The hazard ratio adjusted for age, sex, and co-morbidities for SVA in patients prescribed domperidone compared to PPIs was 0.79 (95% CI 0.25–2.56). There were 44 cases of all-cause cardiac arrest at Xijing and 21 at Guangzhou hospital. Three patients had received domperidone and all had underlying cardiovascular diseases. SVA and cardiac arrest are very rare events in patients prescribed medications for gastrointestinal disorders in China. We found no evidence that domperidone carried a higher risk of SVA compared to other gastrointestinal medications.
- Published
- 2020
45. Study of Ion Permeation through the Graphene Oxide/Polyether Sulfone Membranes
- Author
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Zhe Chen, Sheng Gao Wang, Qiu Ming Fu, Lei Yao, Yongjing Zhang, Zhi Dong Lin, and Xiao Wang
- Subjects
Ion permeation ,Materials science ,Graphene ,Oxide ,Permeation ,Catalysis ,Sulfone ,law.invention ,Dielectric spectroscopy ,chemistry.chemical_compound ,Membrane ,chemistry ,Chemical engineering ,law ,Electrochemistry - Published
- 2020
46. Synergistically ultrahigh energy storage density and efficiency in designed sandwich-structured poly(vinylidene fluoride)-based flexible composite films induced by doping Na0.5Bi0.5TiO3 whiskers
- Author
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Shili Zhan, Ying Lin, Chuang Sun, Yongjing Zhang, Guangliang Hu, Qibin Yuan, and Haibo Yang
- Subjects
Materials science ,Renewable Energy, Sustainability and the Environment ,Whiskers ,Composite number ,Context (language use) ,02 engineering and technology ,General Chemistry ,Dielectric ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Energy storage ,0104 chemical sciences ,Dielectric breakdown model ,General Materials Science ,Composite material ,0210 nano-technology ,Polarization (electrochemistry) ,High-κ dielectric - Abstract
High-performance energy storage dielectrics have been the key to solve energy problems in the context of energy crisis. Designing multilayered structures is an effective approach to break the paradox between high dielectric constant and high breakdown strength existing in polymer-based composite films to enhance energy storage performance. Herein, a designed sandwich-structured poly(vinylidene fluoride) (PVDF)-based composite film, with a characteristic of the inner PVDF layer filled with Na0.5Bi0.5TiO3 (NBT) whiskers and the outer layers of pure PVDF, is proposed. An ultrahigh discharged energy density of 30.55 J cm−3 and an outstanding discharged efficiency of 80.26% can be obtained in the optimized composition with the inner layer containing 6 vol% NBT whiskers, which outperforms all the previously reported PVDF-based composite films. Furthermore, it reveals that such a sandwich structure with NBT whiskers is able to adjust dielectric properties and reform electric field distribution, as evidenced by dielectric and insulation properties as well as the dielectric breakdown model by finite element analysis, which prompts the enhancement of polarization and breakdown strength synergistically, and thus, contributes to excellent energy storage performance. This work provides a strategy for developing high-end dielectrics for electrostatic energy storage applications.
- Published
- 2020
47. An Adaptive Threshold Load Balancing Scheme for the End-to-End Reconfigurable System.
- Author
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Yongjing Zhang, Kui Zhang, Cheng Chi, Yang Ji 0001, Zhiyong Feng 0001, and Ping Zhang 0003
- Published
- 2008
- Full Text
- View/download PDF
48. Thimerosal induces skin pseudo-allergic reaction via Mas-related G-protein coupled receptor B2
- Author
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Jiao Cao, Rui Liu, Yongjing Zhang, Bin Peng, Yi Zheng, Ye Qian, Delu Che, Jue Wang, Langchong He, Songmei Geng, and Y. Hao
- Subjects
Male ,Receptors, Neuropeptide ,0301 basic medicine ,Nerve Tissue Proteins ,Dermatology ,Administration, Cutaneous ,Dermatitis, Contact ,medicine.disease_cause ,Biochemistry ,Cell Degranulation ,Receptors, G-Protein-Coupled ,Proinflammatory cytokine ,Mice ,030207 dermatology & venereal diseases ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Allergen ,medicine ,Animals ,Humans ,Hypersensitivity, Delayed ,Mast Cells ,Molecular Biology ,Mice, Knockout ,Thimerosal ,Preservatives, Pharmaceutical ,Degranulation ,Mast cell ,medicine.disease ,Hypersensitivity reaction ,Disease Models, Animal ,HEK293 Cells ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,Immunology ,Contact dermatitis ,Histamine - Abstract
Background Thimerosal has been used as a preservative in many products which may cause contact dermatitis. It is the second most common allergen in positive patch test reactions, though being a clinical irrelevant allergen. Thimerosal-induced contact dermatitis is generally considered to be a delayed-type hypersensitivity reaction, but it is difficult to explain the fact that most patients develop an allergic reaction upon first encounter with thimerosal. Recent studies have demonstrated the association between Mas-related G protein coupled receptor X2 (MRGPRX2) and pseudo-allergic reactions which occur at the first contact with stimulation. This suggests the possibility that thimerosal may cause contact dermatitis via MRGPRX2 mediated mechanism. Objectives To investigate the role of Mas-related G-protein coupled receptor B2 (MrgprB2)/MRGPRX2 in contact dermatitis induced by thimerosal. Methods Thimerosal induced pseudo-allergic reactions via MrgprB2/ MRGPRX2 were investigated using a novel skin pseudo-allergic reaction mouse model, footpad swelling and extravasation assays in vivo and mast cell degranulation assay in vitro. Results Thimerosal induced contact dermatitis in dorsal skin and footpad swelling in wild-type mice, but had no significant effect in MrgprB2-knockout mice. Thimerosal-induced dermatitis is characterized by infiltration of inflammatory cells and elevation of serum histamine and inflammatory cytokines, rather than elevation of serum IgE level. Thimerosal increased the intracellular Ca2+ concentration in HEK293 cells overexpressing MrgprB2/MRGPRX2. Downregulation of MRGPRX2 resulted in the reduced degranulation of LAD2 human mast cells. Conclusions MrgprB2 mediates thimerosal-induced mast cell degranulation and pseudo-allergic reaction in mice. MRGPRX2 may be a key contributor to human contact dermatitis.
- Published
- 2019
49. Nanoparticle-loaded microcapsules providing effective UV protection for Cry1Ac
- Author
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Aijing Zhang, Mengyuan Li, Yongjing Zhang, Kanglai He, and Shuyuan Guo
- Subjects
Uv protection ,Chitosan ,Materials science ,Alginates ,Organic Chemistry ,Coating materials ,Ultraviolet protection ,Pharmaceutical Science ,Nanoparticle ,Bioengineering ,Capsules ,UV resistance ,chemistry.chemical_compound ,Colloid and Surface Chemistry ,chemistry ,Chemical engineering ,Cry1Ac ,Nanoparticles ,Adsorption ,Physical and Theoretical Chemistry - Abstract
To prepare several novel microcapsules using chitosan (Cs) and Alginate (Alg) as coating materials, and nano-ZnO, nano-SiOMicrocapsules were prepared by the layer-by-layer (LbL) self-assembly technique and electrostatic adsorption. The morphologies were observed by scanning electron microscopy (SEM), and the stability under UV radiation was studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and bioassay.SEM showed that nano-ZnO and nano-TiOThe microcapsules loaded with nanoparticles provided excellent UV resistance for Cry1Ac.
- Published
- 2021
50. Antiviral drugs suppress infection of 2019-nCoV spike pseudotyped virus by interacting with ACE2 protein
- Author
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Zhuoyin Xue, Yanhong Liu, Haoyun Bai, Jue Wang, Yongjing Zhang, Shiling Hu, and Weina Ma
- Subjects
Efavirenz ,Health, Toxicology and Mutagenesis ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,ACE2 ,Peptidyl-Dipeptidase A ,Toxicology ,Biochemistry ,Antiviral Agents ,Virus ,chemistry.chemical_compound ,Zidovudine ,medicine ,Humans ,Viral Pseudotyping ,Receptor ,Molecular Biology ,Research Articles ,EC50 ,chemistry.chemical_classification ,SARS-CoV-2 ,virus diseases ,COVID-19 ,efavirenz ,General Medicine ,Surface Plasmon Resonance ,Virology ,COVID-19 Drug Treatment ,zidovudine ,Molecular Docking Simulation ,Enzyme ,HEK293 Cells ,chemistry ,Molecular Medicine ,Saquinavir ,hormones, hormone substitutes, and hormone antagonists ,Allosteric Site ,2019‐nCoV ,medicine.drug ,Protein Binding ,Research Article - Abstract
The outbreak of coronavirus disease 2019 (COVID‐19) has induced a large number of deaths worldwide. Angiotensin‐converting enzyme 2 (ACE2) is the entry receptor for the 2019 novel coronavirus (2019‐nCoV) to infect the host cells. Therefore, ACE2 may be an important target for the prevention and treatment of COVID‐19. The aim of this study was to investigate the inhibition effect of valaciclovir hydrochloride (VACV), zidovudine (ZDV), saquinavir (SQV), and efavirenz (EFV) on 2019‐nCoV infection. The results of molecule docking and surface plasmon resonance showed that VACV, ZDV, SQV, and EFV could bind to ACE2 protein, with the K D value of (4.33 ± 0.09) e−8, (6.29 ± 1.12) e−6, (2.37 ± 0.59) e−5, and (4.85 ± 1.57) e−5 M, respectively. But only ZDV and EFV prevent the 2019‐nCoV spike pseudotyped virus to enter ACE2‐HEK293T cells with an EC50 value of 4.30 ± 1.46 and 3.92 ± 1.36 μM, respectively. ZDV and EFV also have a synergistic effect on preventing entry of virus into cells. In conclusion, ZDV and EFV suppress 2019‐nCoV infection of ACE2‐HEK293T cells by interacting with ACE2.
- Published
- 2021
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