Search

Your search keyword '"Yoshihara, Tomoki"' showing total 23 results
Start Over Author "Yoshihara, Tomoki"
23 results on '"Yoshihara, Tomoki"'

6. Anti-sortilin1 Antibody Up-Regulates Progranulin via Sortilin1 Down-Regulation

Author

Miyakawa, Shuuichi, primary, Sakuma, Hiroyuki, additional, Warude, Dnyaneshwar, additional, Asanuma, Satomi, additional, Arimura, Naoto, additional, Yoshihara, Tomoki, additional, Tavares, Daniel, additional, Hata, Akito, additional, Ida, Koh, additional, Hori, Yuri, additional, Okuzono, Yuumi, additional, Yamamoto, Syunsuke, additional, Iida, Koichi, additional, Shimizu, Hisao, additional, Kondo, Shinichi, additional, and Sato, Shuji, additional

Published
2020
Full Text
View/download PDF

8. 1161-P: SCO-267, a GPR40 Full Agonist, Improves Glycemic and Body Weight Control More Than That by Fasiglifam in Rat Models of Diabetes and Obesity

Author

MORITOH, YUSUKE, primary, UENO, HIKARU, additional, ITO, RYO, additional, ABE, SHIN-ICHI, additional, MIYASHITA, HIROHISA, additional, OGINO, HITOMI, additional, OOKAWARA, MITSUGI, additional, ISHIMURA, YOSHIMASA, additional, MIYAMOTO, YASUFUMI, additional, YOSHIHARA, TOMOKI, additional, TSUJIHATA, YOSHIYUKI, additional, TAKEUCHI, KOJI, additional, NISHIGAKI, NOBUHIRO, additional, YAMADA, YUKIO, additional, and WATANABE, MASANORI, additional

Published
2019
Full Text
View/download PDF

10. Automated High-Throughput Flow Cytometry for High-Content Screening in Antibody Development

Author

Ding, Mei, Edwards, Bruce S., Wang, Yana, Yoshihara, Tomoki, King, Samson, Le, Tinh, Leroy, Patrick, Zhao, Xiansi, Chan, Ching Kit, Yan, Zhong-Hua, and Menon, Saurabh

Abstract

The tedious sample preparation for flow cytometry limits the throughput and thus its usage as a primary screening method despite its sensitivity and accuracy. With the growing focus on utilizing antibodies as a therapeutic modality in drug discovery, it is critical to develop a high-throughput flow cytometry (HTFC) workflow to cope with the increasing need to support antibody discovery programs. We have developed a seamless HTFC sample preparation and readout workflow using the HighRes modular robotic system and the IntelliCyt iQue Screener PLUS. To fully utilize the advantages offered by flow cytometry, we typically multiplex multiple cell lines of interest in one well to simultaneously quantitate on-target activity and nonspecific activity along with measurement of antibody concentration. The ability to measure multiple parameters coupled with speed and increased accuracy provides gains in productivity and helps speed up antibody lead discovery.

Published
2018
Full Text
View/download PDF

14. Human FcεRIα–Specific Human Single-Chain Fv (scFv) Antibody with Antagonistic Activity toward IgE/FcεRIα-Binding.

Author

Hashiguchi, Shuhei, Nakashima, Toshihiro, Nitani, Aya, Yoshihara, Tomoki, Yoshinaga, Keisuke, Ito, Yuji, Maeda, Yoshio, and Sugimura, Kazuhisa

Subjects
ANTISENSE DNA, ANTISENSE nucleic acids, DNA, ANTISENSE genetics, NUCLEIC acids
Abstract

The α-chain of FcεRI (FcεRIα) plays a critical role in the binding of IgE to FcεRI. A fully human antibody interfering with this interaction may be useful for the prevention of IgE-mediated allergic diseases. Here, we describe the successful isolation of a human single-chain Fv antibody specific to human FcεRIα using human antibody phage display libraries. Using the non-immune phage antibody libraries constructed from peripheral blood lymphocyte cDNA from 20 healthy subjects, we isolated three phage clones (designated as FcRε27, FcRε51, and FcRε70) through two rounds of biopanning selection. The purified soluble scFv, FcRε51, inhibited the binding of IgE to recombinant FcεRIα, although both FcRε27 and FcRε70 showed fine binding specificity to FcεRIα. Since FcRε51 was determined to be a monomer by HPLC, BIAcore analysis was performed. The dissociation constant of FcRε51 to FcεRIα was estimated to be 20 nM, i.e., fortyfold lower than that of IgE binding to FcεRIα (Kd = 0.5 nM). With these characteristics, FcRε51 exhibited inhibitory activity on the release of histamine from passively sensitized human peripheral blood mononuclear cells. [ABSTRACT FROM AUTHOR]

Published
2003
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results