1. Molecular cloning, phylogenetic analysis and heat shock response of Babesia gibsoni heat shock protein 90
- Author
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Reeko Sato, Hiroshi Ohta, Masahiro Yamasaki, Yoshihiro Tsuboi, Kensuke Nakamura, Naohiro Uchida, Mitsuyoshi Takiguchi, and Yusuke Taniyama
- Subjects
0301 basic medicine ,Genetics ,General Veterinary ,biology ,Nucleic acid amplification technique ,biology.organism_classification ,Molecular biology ,03 medical and health sciences ,Open reading frame ,030104 developmental biology ,Heat shock protein ,Complementary DNA ,Babesia ,Coding region ,Heat shock ,Gene - Abstract
The Babesia gibsoni heat shock protein 90 (BgHSP90) gene was cloned and sequenced. The length of the gene was 2,610 bp with two introns. This gene was amplified from cDNA corresponding to full length coding sequence (CDS) with an open reading frame of 2,148 bp. A phylogenetic analysis of the CDS of HSP90 gene showed that B. gibsoni was most closely related to B. bovis and Babesia sp. BQ1/Lintan and lies within a phylogenetic cluster of protozoa. Moreover, mRNA transcription profile for BgHSP90 exposed to high temperature were examined by quantitative real-time reverse transcription-polymerase chain reaction. BgHSP90 levels were elevated when the parasites were incubated at 43°C for 1 hr.
- Published
- 2016
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