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Auxin regulates plant growth and development through downstream signaling pathways, including the best-known SCFTIR1/AFB-Aux/IAA-ARF pathway and several other less characterized "noncanonical" pathways. Recently, one SCFTIR1/AFB-independent noncanonical pathway, mediated by Transmembrane Kinase 1 (TMK1), was discovered through the analyses of its functions in Arabidopsis apical hook development. Asymmetric accumulation of auxin on the concave side of the apical hook triggers DAR1-catalyzed release of the C-terminal of TMK1, which migrates into the nucleus, where it phosphorylates and stabilizes IAA32/34 to inhibit cell elongation, which is essential for full apical hook formation. However, the molecular factors mediating IAA32/34 degradation have not been identified. Here, we show that proteins in the CYTOKININ INDUCED ROOT WAVING 1 (CKRW1)/WAVY GROWTH 3 (WAV3) subfamily act as E3 ubiquitin ligases to target IAA32/34 for ubiquitination and degradation, which is inhibited by TMK1c-mediated phosphorylation. This antagonistic interaction between TMK1c and CKRW1/WAV3 subfamily E3 ubiquitin ligases regulates IAA32/34 levels to control differential cell elongation along opposite sides of the apical hook. [ABSTRACT FROM AUTHOR]

Large-caliber water meter shells are prone to shrinkage porosity, wrinkles, air entrapment, slag inclusion, and other defects during the lost foam casting process. Taking the vertical spiral wing WS-100 water meter as the research object, through the analysis of the structure of the water meter shell, the flow field and temperature field of the design of outer bottom injection-vertical pouring, outer bottom injection-horizontal pouring and inner bottom injection-horizontal pouring involved in the filling and solidification process are numerically simulated and solve analysis, respectively. The results showed that: when the inner bottom injection-horizontal pouring process was adopted, the filling process of the casting was stable, the isolated liquid phase area of the solidification process was relatively small, and the possibility of forming defects was less. The casting performance is excellent, and the numerical analysis is verified to be effective.