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Comprehensive Summary: A label‐free deoxyribozyme (DNAzyme) biosensor for m6A demethylase activity detection is developed. When demethylase FTO (Fat mass and obesity‐associated protein), an important m6A demethylase of ALKBH demethylase family is present, the m6A group is specifically demethylated, and the cleavage activity of DNAzyme is restored. This allows the cleavage product that contains G‐quadruplex sequence to bind to thioflavin T (ThT) and generate fluorescence signals. The biosensor shows high specificity and sensitivity, and fast reaction speed. Our study demonstrates a new design of allosteric DNAzyme for sensing. This method represents the first label‐free nucleic acid biosensor for FTO assay, providing a feasible route towards inhibitors screening. [ABSTRACT FROM AUTHOR]

Four sets of WC-10Co cemented carbides with different carbon content were prepared by adding the ultrafine WC powders as seeds during the in-situ sintering reaction among W, Co and C. The effect of carbon content on microstructure and mechanical properties were studied. The results show that the microstructure, phase composition and mechanical properties of WC-10Co cemented carbides with plate-like WC grains were seriously affected by the carbon content. The fast growth of WC grains with high carbon content could proceed the prismatic plane preferentially along the <1 0 1 (−) 0> directions, resulting in the high content of plate-like WC grains. The density increased with the increment of the carbon content and reached the maximum value, then, followed by a decline. The hardness and the transverse rupture strength of the alloy in the two-phase zone with carbon content of 5.91 wt% reached the maximum value. The existence of plate-like WC grains could impede the propagation of the cracks due to the decrease of the weakest carbide regions and the increase of the basal facets of broken WC crystals. In this case, more fracture energy was required to crack propagation and further improved the transverse rupture strength. Additionally, the plate-like WC was benefit to reduce the wear volume and bring about a better wear resistance. Thus, the alloy with the appropriate proportion of carbon content can obtain higher mechanical properties and wear resistance. [ABSTRACT FROM AUTHOR]