33 results on '"Yu, W.-g."'
Search Results
2. Clinical pharmacist interventions in managing Key Monitoring Drugs in China.
- Author
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YANG, J., ZHENG, L., YU, W.-G., and GU, Y.-C.
- Abstract
OBJECTIVE: Drug-related problems (DRPs) are common in hospitalized patients receiving Key Monitoring Drugs. Clinical pharmacy services have the potential to minimize drug-related harm and improve patient care. The aim of this study is to standardize the clinical application of Key Monitoring Drugs and reduce drug-related problems (DRPs) and associated costs, using clinical pharmacist interventions. PATIENTS AND METHODS: Clinical pharmacists formulate management measures for Key Monitoring Drugs using evidence-based medicine and analyze the DRPs of Key Monitoring Drugs in China at the Shandong Provincial Third Hospital over a period of five years, from 2015 to 2019. RESULTS: In 2019, the total cost of the use of Key Monitoring Drugs decreased by 10.12 million CNY, in comparison with the cost in 2015. The proportion of revenue generated from Key Monitoring Drugs also decreased by 11.49% compared with 2015. In addition, the cost per capita of Key Monitoring Drugs has gradually decreased; this resulted in a saving of 580.07 CNY per capita in 2019 compared with 2015. Over this time, the DRPs associated with Key Monitoring Drugs decreased by 45.50%. Through administrative intervention, prescription review, information management, and pharmaco-economic evaluation, a scientific management system for Key Monitoring Drugs has been established over this time, which standardizes the use of Key Monitoring Drugs and reduces their associated costs. CONCLUSIONS: Clinical pharmacists' interventions can assist in the early detection of drug-related problems associated with Key Monitoring Drugs and prevent any resulting harm to patients. [ABSTRACT FROM AUTHOR]
- Published
- 2021
3. Decreased serum bilirubin is associated with arterial stiffness in men
- Author
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Li, Y., primary, Meng, S.-y., additional, Meng, C.-c., additional, Yu, W.-g., additional, and Wang, R.-t., additional
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- 2013
- Full Text
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4. First Report of Tomato yellow leaf curl virus Infecting Common Bean in China
- Author
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Ji, Y. H., primary, Cai, Z. D., additional, Zhou, X. W., additional, Liu, Y. M., additional, Xiong, R. Y., additional, Zhao, T. M., additional, Yu, W. G., additional, Tao, X. R., additional, and Zhou, Y. J., additional
- Published
- 2012
- Full Text
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5. Modeling of Novel Modulated Helix Induction MEMS Switch on Time-Domain
- Author
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Yu, W G, primary, Zhou, K Q, additional, and Yang, T H, additional
- Published
- 2006
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- View/download PDF
6. Administration of IL-12 induces a CD3+ CD4- CD8- B220+ lymphoid population capable of eliciting cytolysis against Fas-positive tumor cells.
- Author
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Tsutsui, T, primary, Mu, J, additional, Ogawa, M, additional, Yu, W G, additional, Suda, T, additional, Nagaga, S, additional, Saji, F, additional, Murata, Y, additional, Fujiwara, H, additional, and Hamaoka, T, additional
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- 1997
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7. Effect of Macromolecular-Translocation Inhibitor-Ill on Binding of Activated Glucocorticoid-Receptor Complex to Specific DNA
- Author
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Okamoto, K., primary, Liu, G., additional, Yu, W.-G., additional, Ochiai, T., additional, and Isohashi, F., additional
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- 1996
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8. Differential IL-12 responsiveness of T cells but not of NK cells from tumor-bearing mice in IL-12-responsive versus -unresponsive tumor models
- Author
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Iwasaki, M., Yu, W-G., Uekusa, Y., Nakajima, C., Yang, Y-F., Gao, P., Wijesuriya, R., Fujiwara, H., and Hamaoka, T.
- Abstract
While IL-12 administration induces tumor regression through stimulating T cells in tumor-bearing mice, this IL-12 effect is observed in some but not all tumor models. The present study aimed to compare IL-12 responsiveness of T cells from tumor-bearing mice in IL-12-responsive (CSA1M and OV-HM) and -unresponsive (Meth A) tumor models. Tumor regression in IL-12-responsive tumor models required the participation of T cells, but not of NK1.1+ cells. Because a NK1.1+ cell population was the major producer of IFN-γ, comparable levels of IFN-γ production were induced in IL-12-responsive and -unresponsive tumor-bearing mice. This indicates that the amount of IFN-γ produced in tumor-bearing individuals does not correlate with the anti-tumor efficacy of IL-12. In contrast, IL-12 responsiveness of T cells differed between the responsive and unresponsive models: purified T cells from CSA1M/OV-HM-bearing or Meth A-bearing mice exhibited high or low IL-12 responsiveness respectively, when evaluated by the amounts of IFN-γ produced in response to IL-12. T cells from CSA1M- or OV-HM-bearing but not from Meth A-bearing mice exhibited enhanced levels of mRNA for the IL-12 receptor (IL-12R). These results indicate that a fundamental difference exists in IL-12 responsiveness of T cells between IL-12-responsive and -unresponsive tumor models, and that such a difference is associated with the expression of IL-12R on T cells.
- Published
- 2000
9. Molecular mechanisms underlying IFN-gamma-mediated tumor growth inhibition induced during tumor immunotherapy with rIL-12.
- Author
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Yu, W G, Yamamoto, N, Takenaka, H, Mu, J, Tai, X G, Zou, J P, Ogawa, M, Tsutsui, T, Wijesuriya, R, Yoshida, R, Herrmann, S, Fujiwara, H, and Hamaoka, T
- Abstract
The present study investigates the molecular mechanisms by which IFN-gamma produced as a result of in vivo IL-12 administration exerts its anti-tumor effects. rIL-12 was administered three or five times into mice bearing CSA1M fibrosarcoma, OV-HM ovarian carcinoma or MCH-1-A1 fibrosarcoma. This regimen induced complete regression of CSA1M and OV-HM tumors but only transient growth inhibition of MCH-1-A1 tumors. The anti-tumor effects of IL-12 were associated with enhanced induction of IFN-gamma because these effects were abrogated by pretreatment of hosts with anti-IFN-gamma antibody. Exposure in vitro of the three types of tumor cells to rRFN-gamma resulted in moderate to potent inhibition of tumor cell growth. IFN-gamma stimulated the expression of mRNAs for an inducible type of NO synthase (iNOS) in CSA1M cells and indoleamine 2,3-dioxygenase (IDO), an enzyme capable of degrading tryptophan, in OH-HM cells, but induced only marginal levels of these mRNAs in MCH-1-A1 cells. In association with iNOS gene expression, IFN-gamma-stimulated CSA1M cells produced a large amount of NO which functioned to inhibit their own growth in vitro. Although OV-HM and MCH-1A1 cells did not produce NO, they also exhibited NO susceptibility. Whereas the tumor masses from IL-12-treated CSA1M-bearing or OV-HM-bearing mice induced higher levels of iNOS (for CSA1M) or IDO and iNOS (for OV-HM) mRNAs, the MCH-1-A1 tumor mass expressed lower levels of iNOS mRNA alone. Moreover, massive infiltration of CD4(+) and CD8(+) T cells and Mac-1(+) cells was seen only in the CSA1M and OV-HM tumors. Thus, these results indicate that IFN-gamma produced after IL-12 treatment induces the expression of various genes with potential to modulate tumor cell growth by acting directly on tumor cells or stimulating tumor-infiltrating lymphoid cells and that the effectiveness of IL-12 therapy is associated with the operation of these mechanisms.
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- 1996
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10. [The effect on bleeding volume and postoperative recovery of regional cerebral oxygen saturation guides controlled hypotension in elderly patients with hypertension undergoing spinal surgery].
- Author
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Wang L, Li XZ, Yu WG, Dong R, Wang MS, Bi YL, Chu HC, Wang SD, and Li JZ
- Subjects
- Aged, Humans, Oxygen, Postoperative Period, Sevoflurane, Hypertension, Hypotension, Controlled
- Abstract
Objective: To evaluate the effect on bleeding volume and postoperative recovery of regional cerebral oxygen saturation (rSO(2)) guides controlled hypotension in elderly patients with hypertension undergoing spinal surgery. Methods: One hundred and twenty elderly patients who underwent spinal surgery in the department of anesthesiology of Qingdao Municipal Hospital and the Affiliated Hospital of Qingdao University from January 2017 to December 2019 were selected and divided into 2 groups according to the random number table method ( n= 60): rSO(2) guides the controlled hypotension group (group A) and control group (group C). Both groups were performed with endotracheal intubation for general anesthesia, maintain anesthesia with sevoflurane and remifentanil, rSO(2) were monitored throughout the procedure. If necessary, sodium nitroprusside or esmolol were used to control blood pressure. In group A, the goal of controlled hypotension was that rSO(2) decreased ≤ 10% of the basic value or maintained at 64±3 and the moderate operative field bleeding. Group C underwent routine anesthesia management. Intraoperative blood loss and urine output, the incidence of hypothermia after operation, postoperative delirium, chills, nausea and vomiting, the PACU residence time, postoperative drainage volume, eating time, postoperative hospital stay were compared between the two groups. Results: Compared with group C, the blood loss [(589±157) vs (764±213) ml] and urine output [(778±121) vs (1 079±239) ml] of group A were decreased ( t= -5.120, -8.712, all P< 0.05). The rates of hypothermia after operation (26.7% vs 45.0%), postoperative delirium (18.3% vs 36.7%), chills (10.0% vs 25.0%), nausea and vomiting (21.7% vs 40.0%) of group A were decreased (χ(2)=4.385, 5.057, 4.675, 4.728, all P< 0.05) . The PACU residence time [(56±9) vs (63±11) min], postoperative drainage volume [(217±66) vs (289±81) ml], eating time [(17.8±2.8) vs (22.3±4.1) h] and numbers of days in hospital [(7.2±2.7) vs (8.2±2.9) d] were decreased of group A ( t= -3.399, -5.334, -7.000, -2.031, all P< 0.05). Conclusion: The guidance of controlled hypotension with rSO(2) monitoring can reduce the blood loss and infusion volume during spinal surgery in elderly patients with hypertension, reduce postoperative related complications and enhance recovery after surgery.
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- 2020
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11. [Effects of double-catheter epidural analgesia by lidocaine injection respectively on the delivery outcomes and maternal-infant complications for persistent posterior or lateral occipital position of protracted active phase].
- Author
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Li JZ, Wang L, Li XZ, Yu WG, Kang LP, Liu YQ, Ji XH, Wu XF, Wang MS, and Tao H
- Subjects
- Adult, Analgesia, Epidural adverse effects, Analgesia, Obstetrical adverse effects, Female, Humans, Infant, Newborn, Pain, Pregnancy, Pregnancy Outcome, Treatment Outcome, Analgesia, Epidural methods, Analgesia, Epidural statistics & numerical data, Analgesia, Obstetrical methods, Analgesia, Obstetrical statistics & numerical data, Anesthesia, Epidural methods, Cesarean Section statistics & numerical data, Delivery, Obstetric statistics & numerical data, Labor, Obstetric drug effects, Lidocaine administration & dosage
- Abstract
Objective: To evaluate the effect of dual-tube epidural segmental injection of lidocaine analgesia on the delivery outcome and maternal and infant complications of persistent posterior occipital position postpartum or lateral occipital position postpartum patients with protracted active phase. Methods: The full and single-term primiparas ( n =216, 37 to 42 weeks gestation, 22 to 35 years) diagnosed as persistent posterior or lateral occipital position during the active period were selected from the Department of Obstetrics of Qingdao Municipal Hospital from January 2015 to October 2019. The subjects were randomly assigned into two groups: double-tube epidural block group ( n =108) and single-tube epidural block group ( n =108), 1% lidocaine was used for epidural analgesia respectively under ultrasound guidance. Senior midwife or obstetricians implement new partogram, and guide women to perform position management, and push or rotate the fetal head in a timely manner. Observation indicators: general condition, the use of non-pharmacological analgesic measures, analgesia related conditions and pain visual analogue scale (VAS) score, delivery-related indicator, cesarean section indication, anesthesia-related indicator, maternal and child complications. Results: (1) General condition: the age, weight, height, gestational age, the ratio of persistent lateral or posterior occipital position, cephalic score, and neonatal birth weight between the two groups of women were not statistically significant (all P >0.05). (2) The use of non-pharmacological analgesic measures: the women's Lamaze breathing method, Doula delivery companionship, percutaneous electrical stimulation, and other measures between two groups were compared, and there were not significant differences (all P >0.05). (3) Analgesia related conditions and VAS scores of women undergoing vaginal delivery: compared with the single-tube epidural block group ( n =40), the second-partum time of the women in the double-tube epidural block group ( n =59) was significantly shortened [(124±44) vs (86±33) minutes, P <0.01]; after 30 minutes of analgesia (4.4±0.5 vs 0.9±0.5, P <0.01), during forced labor in the second stage of labor (5.7±0.6 vs 1.3±0.4, P <0.01), the VAS scores of pain were also significantly reduced ( P <0.01). (4) Labor-related indicators: compared with the single-tube epidural block group, the natural delivery rate (21.3% vs 49.1%) and the delivery experience satisfaction rate (51.9% vs 98.1%) of women in the double-tube epidural block group were significantly increased (all P <0.01), cesarean section rate (63.0% vs 45.4%), instrument assisted rate (15.7% vs 5.6%) decreased significantly (all P <0.05). (5) Cesarean section indications: compared with the single-tube epidural block group, the cesarean section rate caused by prolonged labor or protracted active phase of women in the double-tube epidural block group was significantly reduced (38.0% vs 22.2%; P <0.05), and the fetal distress, intrauterine infection, and social factors caused by cesarean section between the two groups were compared, while the differences were not statistically significant (all P >0.05).(6) Anesthesia related indexes: the block planes of the maternal upper tube administration in the double-tube epidural block group were mostly T7, T8, T9-L2 and L3,While,the block planes in the single-tube epidural block group were mostly T10, T11-S1, S2, S3, and the modified Bromage score were all 0. (7) Maternal and child complications: compared with the single-tube epidural block group, the postpartum hemorrhage rate (18.5% vs 7.4%), the perineal lateral cut rate (20.4% vs 5.6%), the neonatal asphyxia rate (12.0% vs 3.7%), ICU rate of transferred neonates (13.9% vs 4.6%) in the double-tube epidural block group were significantly reduced (all P <0.05). Soft birth canal injury rate, puerperal disease rate and neonatal birth rate between two groups were compared, and there were not statistically significant differences (all P >0.05). Conclusion: Dual-tube epidural segmental injection of lidocaine analgesia could increase the natural delivery rate of women with posterior occipital or lateral occipital position with active stagnation, reduce the rate of cesarean section and the rate of transvaginal instruments, and reduce the complications of mother and child.
- Published
- 2020
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12. A novel function of Valpha14+CD4+NKT cells: stimulation of IL-12 production by antigen-presenting cells in the innate immune system.
- Author
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Tomura M, Yu WG, Ahn HJ, Yamashita M, Yang YF, Ono S, Hamaoka T, Kawano T, Taniguchi M, Koezuka Y, and Fujiwara H
- Subjects
- Animals, Antigen-Presenting Cells immunology, B-Lymphocytes, CD40 Antigens biosynthesis, CD40 Ligand, Cells, Cultured, Cytokines biosynthesis, Galactosylceramides administration & dosage, Galactosylceramides pharmacology, Immunity, Innate, Immunophenotyping, Injections, Intraperitoneal, Interferon-gamma biosynthesis, Interleukin-12 physiology, Interleukin-4 biosynthesis, Interleukin-4 physiology, Ligands, Lymphocyte Activation drug effects, Lymphocyte Depletion, Membrane Glycoproteins biosynthesis, Mice, Mice, Inbred C57BL, Mice, Knockout, Spleen cytology, Spleen immunology, Antigen-Presenting Cells metabolism, CD4-Positive T-Lymphocytes immunology, Interleukin-12 biosynthesis, Killer Cells, Natural immunology, Receptors, Antigen, T-Cell, alpha-beta immunology, T-Lymphocyte Subsets immunology
- Abstract
The balance between Th1 and Th2 development is determined by IL-4 and IL-12. While the role for CD4+ NK1.1+ T (NKT) cells in influencing this balance has been recognized based on their capacity to produce IL-4, it is unknown how IL-12 is produced in the innate immune system in which they participate. This study demonstrates that Ag-activated CD4+ NKT cells express CD40 ligand (CD40L) (CD154), which engages CD40 on APC and stimulates them to produce IL-12. Culture of B cell-depleted spleen cells from C57BL/6 mice with alpha-galactosylceramide (alpha-GalCer) capable of selectively stimulating Valpha14/Jalpha281+ NKT cells resulted in the production of IL-12 together with IFN-gamma and IL-4. alpha-GalCer-induced IL-12 production occurred in I-Abbeta-deficient mice, but not in beta2-microglobulin-deficient and Valpha14/Jalpha281 TCR-deficient mice, and was inhibited by anti-CD40L mAb. Of CD4+ and CD4- NKT cells, the capacity to express CD40L/CD154 and trigger IL-12 production following alpha-GalCer stimulation was exhibited preferentially by the CD4+ NKT subset. IL-12 production was also observed in alpha-GalCer-treated mice. Production of IL-12 preceded IFN-gamma production, and IL-12 was required for IFN-gamma, but not IL-4, production. A stimulatory/inhibitory relationship existed between IL-12 and IL-4 production. These results illustrate a novel function of CD4+ NKT cells that could be involved in the regulation of Th1 vs Th2 development.
- Published
- 1999
13. A critical role for a peritumoral stromal reaction in the induction of T-cell migration responsible for interleukin-12-induced tumor regression.
- Author
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Ogawa M, Umehara K, Yu WG, Uekusa Y, Nakajima C, Tsujimura T, Kubo T, Fujiwara H, and Hamaoka T
- Subjects
- Animals, Cell Movement, Female, Intercellular Adhesion Molecule-1 analysis, Male, Mice, Mice, Inbred BALB C, Mice, Inbred C3H, Mice, Inbred C57BL, Neoplasms, Experimental immunology, Neoplasms, Experimental therapy, Tumor Cells, Cultured, Vascular Cell Adhesion Molecule-1 analysis, Interleukin-12 therapeutic use, Neoplasms, Experimental blood supply, T-Lymphocytes physiology
- Abstract
Interleukin (IL) 12 has been shown to elicit tumor regression when this cytokine induces the migration of T cells to tumor sites. The present study investigates the role of a peritumoral stromal reaction in IL-12-induced T-cell migration. In the CSA1M and OV-HM tumor models, IL-12 treatment induced tumor regression that is associated with T-cell migration. Neither T-cell migration nor tumor regression was observed in the Meth A and MCH-1-A1 models. Stromal tissue containing neovascular blood vessels developed at the peritumoral area of the former two IL-12-responsive tumors but not at the peritumoral area of the latter two IL-12-unresponsive tumors. The significance of stroma development was investigated using a pair of tumor models (CSA1M and a subline derived from CSA1M designated the CSA1M variant), both of which exhibit the same tumor immunogenicity. In contrast to the parental CSA1M cell line, the variant cell line was not responsive to IL-12, and neither stroma development nor T-cell migration was observed, even after IL-12 treatment. Histological analyses revealed that the parental cell line had peritumoral stroma with intrastromal vessels but only a few vessels in tumor parenchyma, whereas the variant cell line showed no stroma but had abundant vasculature in the tumor parenchyma. Most importantly, only stromal vessels in the parental tumors expressed detectable and enhanced levels of vascular cell adhesion molecule 1 (VCAM-1)/ intercellular adhesion molecule 1 (ICAM-1) before and after IL-12 treatment, respectively. In contrast, parenchymal vasculature in the variant cell line failed to express VCAM-1/ICAM-1 even after IL-12 treatment. When transferred into recipient tumor-bearing mice, IL-12-stimulated T cells from the parental CSA1M-bearing or the variant CSA1M-bearing mice migrated into the parental but not into the variant tumor mass. Together with our previous finding that T-cell migration depends on the VCAM-1/ICAM-1 adhesive interactions, the present results indicate a critical role for peritumoral stroma/stromal vasculature in the acceptance of tumor-infiltrating T cells that is a prerequisite for IL-12-induced tumor regression.
- Published
- 1999
14. Requirement for distinct Janus kinases and STAT proteins in T cell proliferation versus IFN-gamma production following IL-12 stimulation.
- Author
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Ahn HJ, Tomura M, Yu WG, Iwasaki M, Park WR, Hamaoka T, and Fujiwara H
- Subjects
- Animals, Cell Line, Clone Cells, DNA-Binding Proteins metabolism, Interleukin-12 metabolism, Interleukin-2 pharmacology, Janus Kinase 2, Mice, Phosphorylation, Protein-Tyrosine Kinases metabolism, Proteins metabolism, Receptors, Interleukin biosynthesis, Receptors, Interleukin-12, Recombinant Proteins pharmacology, STAT3 Transcription Factor, STAT4 Transcription Factor, STAT5 Transcription Factor, T-Lymphocytes, Helper-Inducer enzymology, T-Lymphocytes, Helper-Inducer metabolism, TYK2 Kinase, Trans-Activators metabolism, Tyrosine metabolism, DNA-Binding Proteins physiology, Interferon-gamma biosynthesis, Interleukin-12 pharmacology, Lymphocyte Activation drug effects, Milk Proteins, Protein-Tyrosine Kinases physiology, Proteins physiology, Proto-Oncogene Proteins, T-Lymphocytes, Helper-Inducer immunology, Trans-Activators physiology
- Abstract
While IL-12 is known to activate JAK2 and TYK2 and induce the phosphorylation of STAT4 and STAT3, little is known regarding how the activation of these signaling molecules is related to the biologic effects of IL-12. Using an IL-12-responsive T cell clone (2D6), we investigated their requirements for proliferation and IFN-gamma production of 2D6 cells. 2D6 cells could be maintained with either IL-12 or IL-2. 2D6 lines maintained with IL-12 (2D6(IL-12)) or IL-2 (2D6(IL-2)) exhibited comparable levels of proliferation, but produced large or only small amounts of IFN-gamma, respectively, when restimulated with IL-12 after starvation of either cytokine. 2D6(IL-12) induced TYK2 and STAT4 phosphorylation. In contrast, their phosphorylation was marginally induced in 2D6(IL-2). The reduced STAT4 phosphorylation was due to a progressive decrease in the amount of STAT4 protein along with the passages in IL-2-containing medium. 2D6(IL-12) and 2D6(IL-2) similarly proliferating in response to IL-12 induced comparable levels of JAK2 activation and STAT5 phosphorylation. JAK2 was associated with STAT5, and IL-12-induced STAT5 phosphorylation was elicited in the absence of JAK3 activation. These results indicate that IL-12 has the capacity to induce/maintain STAT4 and STAT5 proteins, and that TYK2 and JAK2 activation correlate with STAT4 phosphorylation/IFN-gamma induction and STAT5 phosphorylation/cellular proliferation, respectively.
- Published
- 1998
15. [Stretch-activated Ca(2+)-permeable channel].
- Author
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Sokabe M, Yu WG, Qi Z, and Miyazu M
- Subjects
- Animals, Cell Physiological Phenomena, Humans, Membrane Potentials physiology, Calcium Channels physiology, Calcium Signaling physiology, Physical Stimulation
- Published
- 1998
16. Multiple roles of interferon-gamma in the mediation of interleukin 12-induced tumor regression.
- Author
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Ogawa M, Yu WG, Umehara K, Iwasaki M, Wijesuriya R, Tsujimura T, Kubo T, Fujiwara H, and Hamaoka T
- Subjects
- Animals, Antibodies, Monoclonal, Cell Movement drug effects, Down-Regulation, Female, Fibrosarcoma blood supply, Fibrosarcoma drug therapy, Fibrosarcoma pathology, Intercellular Adhesion Molecule-1 biosynthesis, Male, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Neoplasms, Experimental blood supply, Neoplasms, Experimental pathology, Neovascularization, Pathologic, Ovarian Neoplasms blood supply, Ovarian Neoplasms drug therapy, Ovarian Neoplasms pathology, Rats, Remission Induction, T-Lymphocytes drug effects, Tumor Cells, Cultured, Vascular Cell Adhesion Molecule-1 biosynthesis, Antineoplastic Agents therapeutic use, Interferon-gamma physiology, Interleukin-12 therapeutic use, Neoplasms, Experimental drug therapy
- Abstract
Administration of recombinant interleukin 12 (IL-12) induces tumor regression that is associated with T-cell infiltration in the OV-HM ovarian carcinoma and CSA1M fibrosarcoma models. After confirming the blocking of regression by injection of anti-IFN-gamma monoclonal antibody (mAb), we investigated the mechanisms underlying the requirement of IFN-gamma in T-cell migration and tumor regression. T-cell migration was inhibited by injection of anti-IFN-gamma mAb to OV-HM tumor-bearing mice prior to IL-12 treatment. We examined, using the lymphoid cell migration assay, whether IFN-gamma is required for enhancing the migratory capacity of T cells or the T cell-accepting potential of tumor masses during IL-12 treatment. Spleen cells from IL-12-treated or untreated OV-HM-bearing mice were stained in vitro with a fluorescein chemical and transferred i.v. into OV-HM-bearing mice that were not treated with IL-12. Migration of donor cells was quantitated by counting the number of fluorescent cells on cryostat sections of tumor masses from recipient mice. Compared to spleen cells from OV-HM-bearing mice that were not treated with IL-12, enhanced migration was observed for cells from IL-12-treated OV-HM-bearing mice. Anti-IFN-gamma pretreatment of donor mice before IL-12 treatment did not reduce the migratory capacity of T cells, whereas migration was markedly inhibited in recipient mice injected with anti-IFN-gamma. Anti-IFN-gamma pretreatment decreased vascular cell adhesion molecule-1 (VCAM-1)-/intercellular adhesion molecule-1 (ICAM-1)-positive blood vessels at tumor sites. Consistent with this, migration was also inhibited by treatment of recipient mice with either anti-VCAM-1 or anti-ICAM-1 mAb. In contrast to the OV-HM model, T-cell migration was not affected in the CSA1M model following preinjection of anti-IFN-gamma mAb. In this model, VCAM-1-/ICAM-1-positive blood vessels existed even after anti-IFN-gamma treatment, although tumor regression was completely inhibited. These results indicate that IFN-gamma plays two distinct roles in expressing the antitumor efficacy of IL-12: one is to support the T-cell acceptability of tumor masses, and the other is to mediate the antitumor effects of migrated T cells.
- Published
- 1998
17. Strain difference in the induction of T-cell activation-associated, interferon gamma-dependent hepatic injury in mice.
- Author
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Mizuhara H, Kuno M, Seki N, Yu WG, Yamaoka M, Yamashita M, Ogawa T, Kaneda K, Fujii T, Senoh H, and Fujiwara H
- Subjects
- Alanine Transaminase blood, Animals, Cells, Cultured, Concanavalin A pharmacology, Female, Gene Expression, Genetic Variation, Interleukin-2 metabolism, Liver drug effects, Lymphocyte Activation drug effects, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Nitric Oxide Synthase metabolism, Nitric Oxide Synthase Type II, RNA, Messenger analysis, Spleen drug effects, Spleen metabolism, Tumor Necrosis Factor-alpha metabolism, Chemical and Drug Induced Liver Injury etiology, Interferon-gamma metabolism, Liver metabolism, Lymphocyte Activation physiology
- Abstract
A single intravenous injection of concanavalin A (Con A) induces T-cell activation-associated inflammatory injury selectively in the liver. This study investigated the strain difference in the development of Con A-induced hepatic injury. Normal C57BL/6 and BALB/c spleen cells produced comparable levels of T-cell-derived lymphokines (interferon gamma [IFN-gamma], tumor necrosis factor alpha [TNF-alpha], and interleukin-2 [IL-2]) following in vitro stimulation with Con A. A single intravenous injection of Con A to C57BL/6 mice induced the plasma levels of TNF-alpha and IL-2 comparable with or slightly higher than those observed in BALB/c mice, whereas the same treatment resulted in an apparently lower level of IFN-gamma production in C57BL/6 mice. RNA from livers of Con A-treated C57BL/6 mice exhibited lower levels of IFN-gamma mRNA than RNA of BALB/c livers. Unexpectedly, a dramatic difference in the severity of hepatic injury was observed between C57BL/6 and BALB/c. Namely, the peak alanine transaminase (ALT) level was more than 15,000 U/L and inducible as early as 8 hours after injection of 0.2 mg Con A per mouse in the C57BL/6 strain, whereas the peak was approximately 3,000 U/L and induced as late as 24 hours after Con A injection in the BALB/c strain. The increase in plasma ALT levels was limited to less than 10% by injection of anti-IFN-gamma monoclonal antibody (mAb) in both strains. The C57BL/6 strain inducing lower levels of IFN-gamma exhibited higher IFN-gamma responsiveness as exemplified by the intrahepatic expression of an IFN-gamma-inducible gene, an inducible type of nitric oxide (NO) synthase (iNOS). These results indicate that, while IFN-gamma produced in vivo by activated T cells induces hepatic injury, there exists a striking strain difference in the induction of IFN-gamma-dependent hepatic injury.
- Published
- 1998
- Full Text
- View/download PDF
18. Hypotonically induced whole-cell currents in A6 cells: relationship with cell volume and cytoplasmic Ca2+.
- Author
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Yu WG and Sokabe M
- Subjects
- Animals, Cell Line, Chloride Channels antagonists & inhibitors, Cytoplasm drug effects, Osmolar Concentration, Patch-Clamp Techniques, Potassium Channel Blockers, Xenopus laevis, Calcium metabolism, Calcium Channel Blockers pharmacology, Cell Size drug effects, Cytoplasm metabolism, Hypotonic Solutions pharmacology
- Abstract
We investigated changes in whole-cell currents, cell volume, and intracellular calcium concentration ([Ca2+]i) during hypotonic stimulation in whole-cell clamped cultured amphibian renal cells (A6 cells). Upon being exposed to hypotonic solution (80% osmolality), the A6 cells swelled and peaked in the first 5 min, which was followed by a progressive decrease in cell volume termed regulatory volume decrease (RVD). Following the cell swelling, there were large increases in both outward- and inward-currents, which seemed to be carried by K+ efflux and Cl- efflux, respectively. A K+ channel blocker (TEA or quinine) or a Cl- channel blocker (NPPB or SITS) significantly inhibited both currents and RVD, suggesting that the inward- and outward-currents are highly correlated with each other and essential to RVD. Hypotonic stimulation also induced a transient [Ca2+]i increase, of which the time course was essentially similar to that of the currents. When internal and external Ca2+ were deprived to eliminate the Ca2+ transient increase, whole-cell currents and RVD were strongly inhibited. On the other hand, channel blockers TEA and NPPB, which inhibited whole-cell currents and RVD, did not inhibit the [Ca2+]i increase. It is concluded that hypotonic stimulation to A6 cells first induces cell swelling, which is followed by [Ca2+]i increase that leads to the coactivation of K+ and Cl- channels. This coactivation may accelerate K+ and Cl- effluxes, resulting in RVD.
- Published
- 1997
- Full Text
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19. IL-12-induced tumor regression correlates with in situ activity of IFN-gamma produced by tumor-infiltrating cells and its secondary induction of anti-tumor pathways.
- Author
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Yu WG, Ogawa M, Mu J, Umehara K, Tsujimura T, Fujiwara H, and Hamaoka T
- Subjects
- Animals, Chemokine CXCL10, Chemokines biosynthesis, DNA Probes, DNA, Complementary, Fibrosarcoma pathology, Lymphocytes, Tumor-Infiltrating pathology, Male, Mice, Mice, Inbred BALB C, Neutralization Tests, Nitric Oxide Synthase biosynthesis, Polymerase Chain Reaction, RNA, Messenger biosynthesis, Recombinant Proteins therapeutic use, Antibodies, Monoclonal pharmacology, Chemokines, CXC, Fibrosarcoma immunology, Fibrosarcoma therapy, Interferon-gamma biosynthesis, Interferon-gamma immunology, Interleukin-12 therapeutic use, Lymphocytes, Tumor-Infiltrating immunology, Transcription, Genetic drug effects
- Abstract
Administration of recombinant interleukin-12 (rIL-12) into CSA1M fibrosarcoma-bearing mice results in complete regression of growing tumors. This tumor regression is associated with massive lymphoid cell infiltration to tumor sites and is completely blocked by injection of anti-interferon-gamma (IFN-gamma) monoclonal antibody (mAb). We investigated whether anti-IFN-gamma mAb exerts its suppressive effect on tumor regression by blocking the IL-12-induced lymphoid cell migration to tumor sites or by inhibiting the secondary effects of IFN-gamma produced by infiltrating cells. Injection of anti-IFN-gamma mAb to CSA1M-bearing mice before IL-12 treatment prevented the induction of tumor regression, whereas this treatment affected only marginally the infiltration of lymphoid cells to tumor masses. In accordance with this, IFN-gamma mRNA was expressed inside tumor masses by infiltrating cells after IL-12 therapy irrespective of whether anti-IFN-gamma mAb was injected. However, anti-IFN-gamma mAb treatment almost completely abrogated the in situ expression of inducible nitric oxide synthase (iNOS) as well as IFN-inducible protein-10 (IP-10) genes as examples of IFN-gamma-inducible genes. Immunohistochemical analyses also revealed that the expression of iNOS protein was completely inhibited by anti-IFN-gamma injection. These results suggest that the implementation of in situ IFN-gamma activity and its secondary induction of anti-tumor pathways such as iNOS and IP-10 expression are important processes in the IL-12-induced tumor regression.
- Published
- 1997
- Full Text
- View/download PDF
20. [Acetylsalvianolic acid A--a new thromboxane synthetase inhibitor].
- Author
-
Yu WG and Xu LN
- Subjects
- Animals, Blood Platelets metabolism, Dinoprost metabolism, Dinoprostone metabolism, Rabbits, Caffeic Acids pharmacology, Lactates pharmacology, Thromboxane-A Synthase antagonists & inhibitors, Thromboxanes metabolism
- Abstract
With radio thin layer chromatography and autoradiogram, the effect of acetylsalvianolic acid A (ASAA) on 14C-arachidonic metabolism by platelets was studied in vitro. ASAA was found to enhance the formation of PGE2 and PGF2 alpha remarkably while inhibiting the formation of TXB2. However, it showed no effect on the formation of 12-HETE and arachidonic utility rate. Therefore, we deduce that ASAA may be a thromboxane synthetase inhibitor.
- Published
- 1997
21. Enhanced induction of very late antigen 4/lymphocyte function-associated antigen 1-dependent T-cell migration to tumor sites following administration of interleukin 12.
- Author
-
Ogawa M, Tsutsui T, Zou JP, Mu J, Wijesuriya R, Yu WG, Herrmann S, Kubo T, Fujiwara H, and Hamaoka T
- Subjects
- Animals, Antibodies, Blocking immunology, Antibodies, Monoclonal immunology, CD4 Antigens immunology, CD8 Antigens immunology, Cell Movement immunology, Female, Immunohistochemistry, Integrin alpha4beta1, Integrins immunology, Intercellular Adhesion Molecule-1 immunology, Lymphocyte Function-Associated Antigen-1 immunology, Male, Mice, Mice, Inbred BALB C, Mice, Inbred C3H, Mice, Inbred Strains, Neoplasm Transplantation, Receptors, Lymphocyte Homing immunology, Spleen cytology, Spleen transplantation, T-Lymphocyte Subsets immunology, Tumor Cells, Cultured, Vascular Cell Adhesion Molecule-1 immunology, Interleukin-12 pharmacology, Lymphocytes, Tumor-Infiltrating drug effects, Neoplasms, Experimental immunology, T-Lymphocytes drug effects
- Abstract
Administration of interleukin 12 (IL-12) into mice bearing CSA1M, OV-HM, Meth A, or MCH-1-A1 tumor induced complete regression of CSA1M and OV-HM tumors but induced only a slight growth inhibition of Meth A and MCH-1-A1 tumors. These effects of IL-12 were associated with high and only marginal levels of T-cell infiltration into CSA1M/OV-HM and Meth A/MCH-1-A1 tumor masses, respectively. Here, we investigated the role of IL-12 in the induction of T-cell migration. Spleen cells from untreated or IL-12-treated CSA1M-bearing mice were stained in vitro with a fluorescein chemical and transferred i.v. into IL-12-untreated CSA1M-bearing mice. Migration of donor cells was quantitated by counting the number of fluorescent cells on cryostat sections of tumor masses. Although only a slight migration was detected for spleen cells from IL-12-untreated CSA1M-bearing as well as IL-12-treated or untreated normal mice, enhanced migration was observed for cells from IL-12-treated CSA1M-bearing mice. A similar enhanced migration was observed for the OV-HM model. In contrast, such an enhancement was only marginal in the Meth A and MCH-1-A1 models. Immunohistochemical studies of tumors from IL-12-treated mice revealed that the predominant T-cell subset was CD4+ in CSA1M and CD8+ in OV-HM tumor masses. Consistent with this observation, the dominant subset of migrating T cells was found to be CD4+ in the CSA1M and CD8+ in the OV-HM models. T-cell migration was inhibited by pretreatment of recipients with either combination of anti-very late antigen 4 + anti-vascular cell adhesion molecule 1 or anti-lymphocyte function-associated antigen 1 + anti-intercellular adhesion molecule 1 monoclonal antibody. These results indicate that IL-12 can confer T cells with a capacity to migrate to tumor sites through very late antigen 4/lymphocyte function-associated antigen 1 adhesion pathways and that the in vivo acquisition of such a capacity following IL-12 treatment correlates with the induction of tumor regression.
- Published
- 1997
22. Establishment of an IL-12-responsive T cell clone: its characterization and utilization in the quantitation of IL-12 activity.
- Author
-
Maruo S, Ahn HJ, Yu WG, Tomura M, Wysocka M, Yamamoto N, Kobayashi M, Hamaoka T, Trinchieri G, and Fuijiwara H
- Subjects
- Animals, Biological Assay, Cell Aggregation, Cell Division, Cell Line, Cytokines metabolism, Interleukin-12 metabolism, Mice, Mice, Inbred C57BL, Phenotype, Receptors, Interleukin-12, Th1 Cells cytology, Th1 Cells metabolism, Interleukin-12 pharmacology, Receptors, Interleukin metabolism, Th1 Cells drug effects
- Abstract
We previously demonstrated that proliferation of terminally differentiated Th1 clones depends primarily on an interleukin-12 (IL-12)-paracrine mechanism mediated by their interactions with antigen-presenting cells (APC) rather than on an IL-2-autocrine mechanism. Such a Th1 clone (4-86, C57BL/6 origin) was cultured with recombinant IL-12 (rIL-12) in the absence of either antigen or APC. Some cells survived for several passages of culture with only rIL-12, and by limiting dilution, several clones highly reactive to rIL-12 alone were obtained. One of these clones, designated 2D6, was found to proliferate strongly in response to less than 1 pg/mL of rIL-12. This clone exhibited the following surface phenotypes: CD3+, T cell receptor (TCR) alpha beta+, Vbeta11+, NK-1.1-; CD4-CD8-; LFA-1+, ICAM-1+; and CD28+, CD80+, CD86+, CTLA-4-. In accordance with high responsiveness to IL-12, 2D6 cells were also found to express IL-12 receptor (IL-12R) as detected by incubation with rIL-12 and then staining with anti-IL-12 monoclonal antibody (mAb). Stimulation of 2D6 with rIL-12 resulted in the expression of interferon-gamma (IFN-gamma) and IL-10 mRNAs and production of these cytokines. The 2D6 clone responded to IL-2 (vigorously), IL-7 (moderately), and IL-4 (mildly) in addition to IL-12. However, the Ab capture assay using anti-IL-12 mAb enabled us to quantify IL-12-specific activity contained in a given sample. Thus, this study describes the unique features of the IL-12-responsive T cell clone and demonstrates the utilization of this clone in the quantitation of a specific IL-12 activity.
- Published
- 1997
- Full Text
- View/download PDF
23. Immunochemical characterization of the ATP-stimulated glucocorticoid-receptor-translocation promoter from various organs of rat.
- Author
-
Okamoto K, Liu G, Yu WG, and Isohashi F
- Subjects
- Animals, Antibody Specificity, Centrifugation, Density Gradient, Cross Reactions, Cytosol chemistry, Glycerol Kinase, Immunoblotting, Immunochemistry, Kidney chemistry, Liver chemistry, Organ Specificity physiology, Rats, Adenosine Triphosphate pharmacology, Carrier Proteins, DNA-Binding Proteins analysis
- Abstract
We previously found a novel endogenous factor in rat liver cytosol, named ATP-stimulated glucocorticoid-receptor-translocation promoter (ASTP), that increased the binding of activated glucocorticoid-receptor to nuclei in the presence of ATP. In this work, we immunized rabbits with the purified ASTP protein and characterized the antibodies with regard to titer, cross-reactivity and specificity. An IgG fraction from sera of the immunized rabbits contained specific antibodies to ASTP. The anti-ASTP IgG could precipitate the ASTP protein without the activity. Immunoblot analysis revealed a major band of 48 kDa in rat liver cytosol that migrated to the same position as the purified ASTP protein by SDS-PAGE, and an additional minor band of about 50 kDa. Monospecific antibodies purified from the IgG fraction using the antigen (the purified 48-kDa ASTP protein) immobilized on a polyvinylidene difluoride membrane also reacted with both the 48-kDa ASTP protein and the 50-kDa protein in rat liver cytosol, suggesting that this 50-kDa protein is immunologically related to the 48-kDa ASTP protein. Densitometric quantification of immunoblots demonstrated that the rat kidney cytosol contained ASTP protein at a concentration of about 20% of that of liver cytosol. Other tissues such as brain, skeletal muscle, heart, and lung, contained neither the ASTP protein nor the activity.
- Published
- 1994
- Full Text
- View/download PDF
24. Thymic stromal cells eliminate T cells stimulated with antigen plus stromal Ia molecules through their cross-talk involving the production of interferon-gamma and nitric oxide.
- Author
-
Tai XG, Saitoh Y, Satoh T, Yamamoto N, Kita Y, Takenaka H, Yu WG, Zou JP, Hamaoka T, and Fujiwara H
- Subjects
- Cell Communication, Cell Death, Cells, Cultured, DNA Probes, Humans, Interferon-gamma immunology, Interferon-gamma metabolism, Interleukin-2 immunology, Models, Immunological, Nitric Oxide immunology, Nitric Oxide metabolism, Nitric Oxide Synthase biosynthesis, Recombinant Proteins, Stromal Cells cytology, Stromal Cells immunology, Stromal Cells metabolism, T-Lymphocyte Subsets cytology, T-Lymphocyte Subsets immunology, Thymus Gland immunology, Thymus Gland metabolism, Tumor Necrosis Factor-alpha immunology, Histocompatibility Antigens Class II immunology, Receptors, Antigen, T-Cell immunology, T-Lymphocytes immunology, Thymus Gland cytology
- Abstract
We previously established a thymic stromal cell clone capable of inducing differentiation of immature thymocytes and described a clonal elimination model in which T cell clones are killed on the monolayer of this stromal clone by stimulation of their T cell receptors (TCR) with antigen plus stromal Ia molecules. This study investigated molecular mechanisms underlying this phenomenon. Antigenic stimulation on thymic stromal cells produced large amounts of interferon-gamma (IFN-gamma) and small amounts of tumor necrosis factor-alpha (TNF-alpha). Addition of anti-IFN-gamma monoclonal antibody (mAb) to these cultures largely prevented death of TCR-stimulated T cells. T cell death was also induced when cultures were treated with recombinant IFN-gamma (rIFN-gamma) or rTNF-alpha instead of the relevant antigen, showing that these lymphokines are involved in the process of T cell death. It was further demonstrated that these lymphokines, especially IFN-gamma, induced the expression of mRNA for the inducible type of nitric oxide (NO) synthase in thymic stromal cells and that enhanced levels of NO were produced by stromal cells cultures with T cells plus antigen or stimulated with rIFN-gamma or rTNF-alpha. NO was found to be critically responsible for inducing T cell death on the stromal cell monolayer following stimulation of T cells with antigen or of stromal cells with rIFN-gamma or rTNF-alpha, because T cells death was completely prevented by addition of NG-monomethyl-L-arginine (L-NMMA), which is capable of inhibiting NO production. These results indicate that elimination of TCR-stimulated T cells on thymic stromal monolayers with the capacity to support thymocyte differentiation is induced by the cross-talk between IFN-gamma/TNF-alpha-producing T cells and stromal cells capable of producing NO in response to these lymphokines.
- Published
- 1994
25. [Effect of acetylsalvianolic acid A on platelet function].
- Author
-
Yu WG and Xu LN
- Subjects
- Animals, Blood Platelets metabolism, Male, Rabbits, Rats, Serotonin blood, Caffeic Acids pharmacology, Lactates pharmacology, Platelet Aggregation drug effects, Platelet Aggregation Inhibitors pharmacology
- Abstract
Acetylsalvianolic acid A (ASAA) is an semisynthetic analogue of salvianolic acid A, isolated from Danshen (Salvia miltiorrhiza Bunge). In in vitro experiments, ASAA showed marked inhibitory effect on rat and rabbit platelet aggregation induced by ADP, collagen, arachidonic acid (AA) and thrombin. In ex vivo experiments with ADP, collagen, and AA as inducers, ASAA was also shown to inhibit platelet aggregation remarkably. The effect lasted more than two hours. In addition, ASAA was found to have suppressive effect on collagen induced platelet 5-HT release while inhibiting aggregation. The above results seemed to suggest that ASAA may be a widely effective inhibitor of platelet function.
- Published
- 1994
26. [Triggered activities in cat heart induced by combined administration of ouabain and calcium gluconate].
- Author
-
Yu WG and Xie JT
- Subjects
- Action Potentials drug effects, Animals, Arrhythmias, Cardiac chemically induced, Cats, Drug Synergism, Electrophysiology, Female, Male, Calcium Gluconate pharmacology, Heart physiology, Ouabain pharmacology
- Abstract
The pharmacological effects of i.v. a mixture of ouabain (Oua) (50 micrograms.kg-1) and calcium gluconate (Ca2+) (100 mg.kg-1) on the electric activities of the cat hearts (n = 19) were studied by using a contact electrode and a contact electrode catheter to record both epicardial and endocardial monophasic action potentials (MAP). The results showed that the 2 drugs together reduced the MAP amplitude, decreased Vmax, and lengthened the MAP duration. Ouabain induced triggered activities, eg, early afterdepolarization, early afterhyperpolarization, delayed afterdepolarization including oscillatory afterpotentials, and triggered arrhythmias, which were enhanced by calcium gluconate. Endocardium is more sensitive than epicardium to ouabain.
- Published
- 1993
27. Formation of monohydroxy derivatives of arachidonic acid, linoleic acid, and oleic acid during oxidation of low density lipoprotein by copper ions and endothelial cells.
- Author
-
Wang T, Yu WG, and Powell WS
- Subjects
- Cells, Cultured, Chromatography, High Pressure Liquid, Fatty Acids metabolism, Gas Chromatography-Mass Spectrometry, Humans, Kinetics, Linoleic Acid, Lipid Peroxidation, Male, Oleic Acid, Oxidation-Reduction, Spectrophotometry, Ultraviolet, Arachidonic Acids metabolism, Copper metabolism, Endothelium, Vascular metabolism, Linoleic Acids metabolism, Lipoproteins, LDL metabolism, Oleic Acids metabolism
- Abstract
An important event in the formation of atherosclerotic lesions is the uptake of modified low density lipoprotein (LDL) by macrophages via scavenger receptors. Modification of LDL, which results in its recognition by these receptors, can be initiated by peroxidation of LDL lipids. The first step in this process is the formation of monohydroperoxy derivatives of fatty acids, which are subsequently degraded to the corresponding monohydroxy compounds, or to a variety of secondary oxidation products. In order to understand this process more completely, we have developed a mass spectrometric procedure to measure the amounts of specific hydroperoxy/hydroxy fatty acids formed by oxidation of the major unsaturated fatty acids in human LDL, oleic acid, linoleic acid, and arachidonic acid. Oxidation of human LDL in the presence of a relatively strong stimulus (20 microM CuSO4) resulted in very large increases in the amounts of the major monohydroxy derivatives of linoleic acid (9- and 13-hydroxy derivatives) and arachidonic acid (5-, 8-, 9-, 11-, 12-, and 15-hydroxy derivatives) in LDL lipids in the early stages of the reaction. After 20 h, the amounts of these products declined due to substrate depletion, but large amounts of monohydroxy derivatives of oleic acid (8-, 10-, and 11-hydroxy derivatives) were detected. Although thiobarbituric acid-reactive substances clearly increased under these conditions, the changes were not nearly so dramatic as those observed for monohydroxy fatty acids. Oxidation of LDL in the presence of endothelial cells, a much milder stimulus, resulted in 2.5- to 3-fold increases in the amounts of monohydroxy derivatives of linoleic and arachidonic acids, as well as thiobarbituric acid-reactive substances, with more modest increases in the amounts of hydroxylated derivatives of oleic acid. There was little positional specificity in the oxidation of the above fatty acids in the presence of either stimulus, suggesting that the formation of these products proceeds primarily by lipid peroxidation, rather than by catalysis by lipoxygenases. However, an important role for lipoxygenases in the initiation of these reactions cannot be excluded. In conclusion, oxidation of LDL in the presence of copper ions or endothelial cells results in the formation of a large number of monohydroxy derivatives of oleic, linoleic, and arachidonic acids. The relative amounts of products formed from each of these fatty acids depends on the strength of the stimulus as well as the incubation time.
- Published
- 1992
28. [Highlights of thromboxane synthetase inhibitor research].
- Author
-
Yu WG and Xu LN
- Subjects
- Animals, Epoprostenol biosynthesis, Humans, Imidazoles, Methacrylates, Thromboxane A2 physiology, Thromboxane-A Synthase physiology, Platelet Aggregation drug effects, Thromboxane-A Synthase antagonists & inhibitors
- Published
- 1990
29. [Fetal Doppler echocardiography].
- Author
-
Yu WG, Kang ZH, and Zhang JY
- Subjects
- Female, Fetal Heart physiology, Humans, Pregnancy, Echocardiography, Doppler, Fetal Diseases diagnosis, Heart Defects, Congenital diagnosis, Prenatal Diagnosis
- Published
- 1990
30. [Effect of sodium ferulate on arachidonic acid metabolism].
- Author
-
Xu LN, Yu WG, Tian JY, and Liu QY
- Subjects
- 6-Ketoprostaglandin F1 alpha blood, Animals, Aorta metabolism, Blood Platelets drug effects, Dinoprost blood, Dinoprostone blood, Male, Rabbits, Rats, Thromboxane B2 blood, Anticoagulants pharmacology, Arachidonic Acids metabolism, Coumaric Acids pharmacology
- Abstract
Sodium ferulate (SF) is one of the antiplatelet ingredients in Radix Angleica sinensis. The effect of SF on 14C-arachidonic acid metabolism in washed intact rabbit platelets was studied with radiochromatography and radioautography. SF (0.1-3.2 mmol/L) inhibited the generation of platelet thromboxane B2 in a dose-dependent manner (reduced by 16.7-93.8%) and the IC50 was shown to be 0.762 mmol/L. Simultaneously with the reduction of TXB2 generation, the formation of PGE2 and PGF2 alpha was also reduced significantly after treatment with SF. Using radioimmunoassay SF (0.145-2.32 mmol/L) was found to inhibit rabbit platelet TXB2 formation in a dose-dependent manner. SF (0.58-2.32 mmol/L) also suppressed aortic tissue 6-keto-PGF1 alpha generation in rabbits. At the same concentrations the inhibitory effect of SF on platelet TXB2 formation was greater than that on aortic tissue 6-keto-PGF1 alpha generation. These results indicate that the cyclo-oxygenase activity may be inhibited by SF.
- Published
- 1990
31. [Effect of sodium ferulate on C14-arachidonic acid metabolism in rabbit platelets].
- Author
-
Xu LN, Yu WG, and Tian JY
- Subjects
- Animals, Blood Platelets metabolism, Dinoprost biosynthesis, Dinoprostone biosynthesis, Platelet Aggregation drug effects, Rabbits, Thromboxane B2 biosynthesis, Arachidonic Acids metabolism, Cinnamates pharmacology, Coumaric Acids pharmacology
- Published
- 1988
32. Intestinal polyposis of 13 cases.
- Author
-
Zhang SQ and Yu WG
- Subjects
- Adolescent, Adult, Child, Female, Humans, Infant, Intestinal Neoplasms genetics, Intestinal Neoplasms surgery, Intestinal Polyps genetics, Intestinal Polyps surgery, Male, Middle Aged, Intestinal Neoplasms pathology, Intestinal Polyps pathology
- Published
- 1983
33. [Intestinal polyposis].
- Author
-
Zhang SQ and Yu WG
- Subjects
- Adenomatous Polyposis Coli epidemiology, Adult, Female, Humans, Male, Peutz-Jeghers Syndrome surgery, Adenomatous Polyposis Coli surgery
- Published
- 1986
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