8 results on '"Yu-Sung Hsu"'
Search Results
2. Robust induction of TRMs by combinatorial nanoshells confers cross-strain sterilizing immunity against lethal influenza viruses
- Author
-
Pin-Hung Lin, Chieh-Yu Liang, Bing-Yu Yao, Hui-Wen Chen, Ching-Fu Pan, Li-Ling Wu, Yi-Hsuan Lin, Yu-Sung Hsu, Yu-Han Liu, Pei-Jer Chen, Che-Ming Jack Hu, and Hung-Chih Yang
- Subjects
resident memory T cell ,nanoshell ,T cell vaccine ,peptide vaccine ,influenza virus ,universal influenza vaccine ,Genetics ,QH426-470 ,Cytology ,QH573-671 - Abstract
Antigen-specific lung-resident memory T cells (TRMs) constitute the first line of defense that mediates rapid protection against respiratory pathogens and inspires novel vaccine designs against infectious pandemic threats, yet effective means of inducing TRMs, particularly via non-viral vectors, remain challenging. Here, we demonstrate safe and potent induction of lung-resident TRMs using a biodegradable polymeric nanoshell that co-encapsulates antigenic peptides and TLR9 agonist CpG-oligodeoxynucleotide (CpG-ODN) in a virus-mimicking structure. Through subcutaneous priming and intranasal boosting, the combinatorial nanoshell vaccine elicits prominent lung-resident CD4+ and CD8+ T cells that surprisingly show better durability than live viral infections. In particular, nanoshells containing CpG-ODN and a pair of conserved class I and II major histocompatibility complex-restricted influenza nucleoprotein-derived antigenic peptides are demonstrated to induce near-sterilizing immunity against lethal infections with influenza A viruses of different strains and subtypes in mice, resulting in rapid elimination of replicating viruses. We further examine the pulmonary transport dynamic and optimal composition of the nanoshell vaccine conducive for robust TRM induction as well as the benefit of subcutaneous priming on TRM replenishment. The study presents a practical vaccination strategy for inducing protective TRM-mediated immunity, offering a compelling platform and critical insights in the ongoing quest toward a broadly protective vaccine against universal influenza as well as other respiratory pathogens.
- Published
- 2021
- Full Text
- View/download PDF
3. Development of a color photo-curable 3D printing mechatronics system by using multiple piezoelectric heads.
- Author
-
Ming-Jong Tsai, Yu-Sung Hsu, Yih-Lin Cheng, Freeman Chen, and Chih-Hsuan Chang
- Published
- 2016
- Full Text
- View/download PDF
4. Costimulatory domains direct distinct fates of CAR-driven T cell dysfunction
- Author
-
Mehmet Emrah Selli, Jack Landmann, Marina Terekhova, John Lattin, Amanda Heard, Yu-Sung Hsu, Tien-Ching Chang, Ju-fang Chang, John M Warrington, Helen Ha, Natalie L Kingston, Graham Hogg, Michael Slade, Melissa M Berrien-Elliott, Mark Foster, Samantha Kersting-Schadek, Agata Gruszczynska, David DeNardo, Todd A Fehniger, Maxim Artyomov, and Nathan Singh
- Subjects
Immunology ,Cell Biology ,Hematology ,Biochemistry ,Article - Abstract
T cells engineered to express chimeric antigen receptors (CARs) targeting CD19 have demonstrated impressive activity against relapsed or refractory B cell cancers yet fail to induce durable remissions for nearly half of patients treated. Enhancing the efficacy of this therapy requires detailed understanding of the molecular circuitry that restrains CAR-driven anti-tumor T cell function. We developed and validated an in vitro model that drives T cell dysfunction through chronic CAR activation and interrogated how CAR costimulatory domains, central components of CAR structure and function, contribute to T cell failure. We found that chronic activation of CD28-based CARs results in activation of classical T cell exhaustion programs and development of dysfunctional cells that bear the hallmarks of exhaustion. In contrast, 41BB-based CARs activate a divergent molecular program and direct differentiation of T cells into a novel cell state. Interrogation of CAR T cells from a patient with progressive lymphoma confirmed activation of this novel program in a failing clinical product. Further, we demonstrate that 41BB-dependent activation of the transcription factor FOXO3 is directly responsible for impairing CAR T cell function. These findings identify that costimulatory domains are critical regulators of CAR-driven T cell failure and that targeted interventions are required to overcome costimulation-dependent dysfunctional programs.
- Published
- 2023
5. Antigen-specific depletion of CD4(+) T cells by CAR T cells reveals distinct roles of higher and lower affinity TCRs during autoimmunity
- Author
-
Jaeu Yi, Aidan T. Miller, Angela S. Archambault, Andrew J. Jones, Tara R. Bradstreet, Sravanthi Bandla, Yu-Sung Hsu, Brian T. Edelson, You W. Zhou, Daved H. Fremont, Takeshi Egawa, Nathan Singh, Gregory F. Wu, and Chyi-Song Hsieh
- Subjects
CD4-Positive T-Lymphocytes ,Mice ,Encephalomyelitis, Autoimmune, Experimental ,Receptors, Chimeric Antigen ,Immunology ,Receptors, Antigen, T-Cell ,Animals ,Autoimmunity ,General Medicine ,Antigens ,Peptides ,Article - Abstract
Both higher- and lower-affinity self-reactive CD4 + T cells are expanded in autoimmunity; however, their individual contribution to disease remains unclear. We addressed this question using peptide-MHCII chimeric antigen receptor (pMHCII-CAR) T cells to specifically deplete peptide-reactive T cells in mice. Integration of improvements in CAR engineering with TCR repertoire analysis was critical for interrogating in vivo the role of TCR affinity in autoimmunity. Our original MOG 35–55 pMHCII-CAR, which targeted only higher-affinity TCRs, could prevent the induction of experimental autoimmune encephalomyelitis (EAE). However, pMHCII-CAR enhancements to pMHCII stability, as well as increased survivability via overexpression of a dominant-negative Fas, were required to target lower-affinity MOG-specific T cells and reverse ongoing clinical EAE. Thus, these data suggest a model in which higher-affinity autoreactive T cells are required to provide the “activation energy” for initiating neuroinflammatory injury, but lower-affinity cells are sufficient to maintain ongoing disease.
- Published
- 2022
6. Robust induction of TRMs by combinatorial nanoshells confers cross-strain sterilizing immunity against lethal influenza viruses
- Author
-
Li Ling Wu, Hui-Wen Chen, Ching Fu Pan, Yu Han Liu, Bing Yu Yao, Hung-Chih Yang, Chieh Yu Liang, Yu Sung Hsu, Che Ming Jack Hu, Pin Hung Lin, Yi Hsuan Lin, and Pei-Jer Chen
- Subjects
0301 basic medicine ,Priming (immunology) ,Biology ,QH426-470 ,influenza virus ,03 medical and health sciences ,0302 clinical medicine ,Immunity ,peptide vaccine ,Genetics ,universal influenza vaccine ,nanoshell ,Molecular Biology ,T cell vaccine ,QH573-671 ,resident memory T cell ,Respiratory infection ,TLR9 ,Virology ,Vaccination ,030104 developmental biology ,030220 oncology & carcinogenesis ,Peptide vaccine ,Molecular Medicine ,Cytology ,T-cell vaccine ,CD8 - Abstract
Antigen-specific lung-resident memory T cells (TRMs) constitute the first line of defense that mediates rapid protection against respiratory pathogens and inspires novel vaccine designs against infectious pandemic threats, yet effective means of inducing TRMs, particularly via non-viral vectors, remain challenging. Here, we demonstrate safe and potent induction of lung-resident TRMs using a biodegradable polymeric nanoshell that co-encapsulates antigenic peptides and TLR9 agonist CpG-oligodeoxynucleotide (CpG-ODN) in a virus-mimicking structure. Through subcutaneous priming and intranasal boosting, the combinatorial nanoshell vaccine elicits prominent lung-resident CD4+ and CD8+ T cells that surprisingly show better durability than live viral infections. In particular, nanoshells containing CpG-ODN and a pair of conserved class I and II major histocompatibility complex-restricted influenza nucleoprotein-derived antigenic peptides are demonstrated to induce near-sterilizing immunity against lethal infections with influenza A viruses of different strains and subtypes in mice, resulting in rapid elimination of replicating viruses. We further examine the pulmonary transport dynamic and optimal composition of the nanoshell vaccine conducive for robust TRM induction as well as the benefit of subcutaneous priming on TRM replenishment. The study presents a practical vaccination strategy for inducing protective TRM-mediated immunity, offering a compelling platform and critical insights in the ongoing quest toward a broadly protective vaccine against universal influenza as well as other respiratory pathogens.
- Published
- 2021
7. Robust induction of T
- Author
-
Pin-Hung, Lin, Chieh-Yu, Liang, Bing-Yu, Yao, Hui-Wen, Chen, Ching-Fu, Pan, Li-Ling, Wu, Yi-Hsuan, Lin, Yu-Sung, Hsu, Yu-Han, Liu, Pei-Jer, Chen, Che-Ming Jack, Hu, and Hung-Chih, Yang
- Subjects
T cell vaccine ,respiratory infection ,resident memory T cell ,peptide vaccine ,universal influenza vaccine ,Original Article ,nanoshell ,influenza virus - Abstract
Antigen-specific lung-resident memory T cells (TRMs) constitute the first line of defense that mediates rapid protection against respiratory pathogens and inspires novel vaccine designs against infectious pandemic threats, yet effective means of inducing TRMs, particularly via non-viral vectors, remain challenging. Here, we demonstrate safe and potent induction of lung-resident TRMs using a biodegradable polymeric nanoshell that co-encapsulates antigenic peptides and TLR9 agonist CpG-oligodeoxynucleotide (CpG-ODN) in a virus-mimicking structure. Through subcutaneous priming and intranasal boosting, the combinatorial nanoshell vaccine elicits prominent lung-resident CD4+ and CD8+ T cells that surprisingly show better durability than live viral infections. In particular, nanoshells containing CpG-ODN and a pair of conserved class I and II major histocompatibility complex-restricted influenza nucleoprotein-derived antigenic peptides are demonstrated to induce near-sterilizing immunity against lethal infections with influenza A viruses of different strains and subtypes in mice, resulting in rapid elimination of replicating viruses. We further examine the pulmonary transport dynamic and optimal composition of the nanoshell vaccine conducive for robust TRM induction as well as the benefit of subcutaneous priming on TRM replenishment. The study presents a practical vaccination strategy for inducing protective TRM-mediated immunity, offering a compelling platform and critical insights in the ongoing quest toward a broadly protective vaccine against universal influenza as well as other respiratory pathogens., Graphical Abstract, Nanoshell vaccines encapsulating CpG and both MHC-I and MHC-II-restricted conserved peptides derived from influenza virus proteomes can elicit robust CD4+ and CD8+ lung-resident memory T cells (TRMs) and circulatory memory T cells through the subcutaneous priming and intranasal boosting strategy to protect against influenza viruses of different strains and subtypes.
- Published
- 2020
8. Development of a color photo-curable 3D printing mechatronics system by using multiple piezoelectric heads
- Author
-
Cheng Yih-Lin, Freeman Chen, Yu-Sung Hsu, Ming-Jong Tsai, and Chih-Hsuan Chang
- Subjects
0209 industrial biotechnology ,Engineering drawing ,Computer science ,business.industry ,Process (computing) ,3D printing ,02 engineering and technology ,Mechatronics ,021001 nanoscience & nanotechnology ,020901 industrial engineering & automation ,Color mixing ,Three dimensional printing ,Digital manufacturing ,0210 nano-technology ,business ,Design technology - Abstract
The 3D printing, also called additive manufacturing, is a combination of three-dimensional digital design technology, material science, digital manufacturing and intelligent control of a mechatronics system etc. The main purpose of this study is to develop a color photo-curable 3D manufacturing system by using multiple Piezoelectric heads. Four different colors (CMYW) of curable materials (UV resin) were developed first. This study has finished system integration of color mixing, piezo material process, CMYW curable process of and multi-inkjet head control etc. The proposed system can provide colorful 3D object printing solutions. From the experimental results with multiple piezo-heads (150dpi each), the developed color 3D printing technique can achieve the resolution of 300 dpi and 256 colors. This study also complies with the future development trend and market's requirements of color 3D additive manufacturing product.
- Published
- 2016
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.