Search

An enzyme cascade system including glucose oxidase (GOx) and iron porphyrin (DhHP‐6) is encapsulated in a metal–organic framework called zeolitic imidazolate framework‐8 (ZIF‐8) through one‐step facile synthesis. The composite (GOx&DhHP‐6@ZIF‐8) is then used to initiate oxygen‐tolerant reversible addition‐fragmentation chain‐transfer polymerization for different methacrylate monomers, such as 2‐diethylaminoethyl methacrylate, 2‐hydroxyethyl methacrylate, and poly(ethylene glycol) methyl ether methacrylate (Mn = 500 g mol−1). The composite shows the robustness toward solvent and temperatures, all polymerizations using above monomers and catalyzing by GOx&DhHP‐6@ZIF‐8 exhibits high monomer conversion (>85%) and narrow molar mass dispersity (<1.3). Besides, acrylic and acrylamide monomers such as 2‐hydroxyethyl acrylate and N,N‐dimethylacrylamide are also carried to demonstrate the broad applicability. Proton nuclear magnetic resonance characterization and chain extension experiments confirm the retaining end groups of the resultant polymers, which is a significant feature of living polymerization. More importantly, the process of recycling the composite through a centrifuge is simplistic, and the composite still maintains similar activity compared to the original composites after five times. This low‐cost and easily separated composite catalyst represents a versatile strategy to synthesize well‐defined functional polymers suitable for industrial‐scale production. [ABSTRACT FROM AUTHOR]

The hyperglycemic environment and the presence of bacterial infections delay the healing of diabetic wounds. Herein, glucose oxidase (GOx) and Fe3O4/TiO2/Ag3PO4 were embedded in a polyacrylic acid-calcium phosphate (PAA-CaPs@Nps@GOx) hydrogel through an in situ biomimetic mineralization approach. The GOx encapsulation efficiency was 96.75% and exhibited exceptional enzyme activity stability. Moreover, the co-immobilization of GOx and Fe3O4/TiO2/Ag3PO4 nanoparticles generated a simple and multifunctional antibacterial platform with the advantages of decreasing blood glucose concentration and efficiently producing reactive oxygen species (ROS). In addition, the degradation rate of the hydrogel was controlled by regulating the concentration of phosphate thus controlling the release of Fe3O4/TiO2/Ag3PO4 and GOx. As a result, both the potential toxicity and oxidative stress associated with the antimicrobial biomaterial can be controlled within the body therefore potentially preventing detriment. In vivo results indicated that the PAA-CaPs@Nps@GOx hydrogel effectively promoted diabetic wound healing and showed great potential for clinical applications of chronic wound management. [ABSTRACT FROM AUTHOR]

The hyperglycemic environment and the presence of bacterial infections delay the healing of diabetic wounds. Herein, glucose oxidase (GOx) and Fe 3 O 4 /TiO 2 /Ag 3 PO 4 were embedded in a polyacrylic acid-calcium phosphate (PAA-CaPs@Nps@GOx) hydrogel through an in situ biomimetic mineralization approach. The GOx encapsulation efficiency was 96.75% and exhibited exceptional enzyme activity stability. Moreover, the co-immobilization of GOx and Fe 3 O 4 /TiO 2 /Ag 3 PO 4 nanoparticles generated a simple and multifunctional antibacterial platform with the advantages of decreasing blood glucose concentration and efficiently producing reactive oxygen species (ROS). In addition, the degradation rate of the hydrogel was controlled by regulating the concentration of phosphate thus controlling the release of Fe 3 O 4 /TiO 2 /Ag 3 PO 4 and GOx. As a result, both the potential toxicity and oxidative stress associated with the antimicrobial biomaterial can be controlled within the body therefore potentially preventing detriment. In vivo results indicated that the PAA-CaPs@Nps@GOx hydrogel effectively promoted diabetic wound healing and showed great potential for clinical applications of chronic wound management.