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318 results on '"Yuan, Shoujun"'

36. Surface Engineering of HEK293 Cell-Derived Extracellular Vesicles for Improved Pharmacokinetic Profile and Targeted Delivery of IL-12 for the Treatment of Hepatocellular Carcinoma

Author

Zhang,Jing, Song,Haijing, Dong,Yanan, Li,Ganghui, Li,Jun, Cai,Qizhe, Yuan,Shoujun, Wang,Yi, Song,Haifeng, Zhang,Jing, Song,Haijing, Dong,Yanan, Li,Ganghui, Li,Jun, Cai,Qizhe, Yuan,Shoujun, Wang,Yi, and Song,Haifeng

Abstract

Jing Zhang,1 Haijing Song,2 Yanan Dong,1 Ganghui Li,3 Jun Li,1 Qizhe Cai,4 Shoujun Yuan,5 Yi Wang,1 Haifeng Song1 1State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing, 102206, People’s Republic of China; 2Emergency Medicine, PLA Strategic Support Force Medical Center, Beijing, 100101, People’s Republic of China; 3China Pharmaceutical University, Nanjing, 211198, People’s Republic of China; 4Department of Echocardiography, Beijing Chao Yang Hospital, Capital Medical University, Beijing, 100020, People’s Republic of China; 5Beijing Institute of Radiation Medicine, Beijing, 100850, People’s Republic of ChinaCorrespondence: Haifeng Song; Yi Wang, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing, 102206, People’s Republic of China, Tel +86 10 81139169, Fax +86 10 81139169, Email songhf@vip.163.com; yves.wangy@vip.163.comBackground: Extracellular vesicles (EVs) are considered a promising drug delivery platform. Naïve EVs face numerous issues that limit their applications, such as fast clearance, hepatic accumulations, and a lack of target-specific tropism. We aimed to explore a series of surface engineering approaches to: 1) reduce the non-specific adhesion of EVs, and 2) improve their enrichment in the target tissue. As a proof-of-concept, we investigated the therapeutic potentials of a multi-modal EVs system carrying a tumor-specific nanobody and the immuno-stimulant interleukin-12 (IL12) using in vivo models of hepatocellular carcinoma.Methods: The major cell adhesion molecule on the HEK293-derived EVs, integrin β 1 (ITGB1), was knocked out (KO) by CRISPR/Cas9-mediated gene editing, followed by deglycosylation to generate ITGB1−Deg EVs for the subsequent pharmacokinetic and biodistribution analyses. ITGB1−Deg EVs were further loaded with glypican-3 (GPC3)-specific nanobody (HN3) and mouse single-chain IL12 (m

Published
2023

48. Modification of TiO2 by Er3+ and rGO enhancing visible photocatalytic degradation of arsanilic acid.

Author

Ren, Xinghao, Yao, Hang, Tang, Rui, A, Rong, Yuan, Shoujun, Wang, Wei, Ali, Ibrahim Mohamed, and Hu, Zhen-Hu

Subjects
PHOTODEGRADATION, ARSENIC, VISIBLE spectra, REACTIVE oxygen species, BAND gaps, GRAPHENE oxide, ERBIUM compounds
Abstract

As a typical wide band gap photocatalyst, titania (TiO2) cannot use the visible light and has fast recombination rate of photogenerated electron–hole pairs. Simultaneous introduction of erbium ion (Er3+) and graphene oxide (rGO) into TiO2 might overcome these two drawbacks. In this study, Er3+ and rGO were co-doped on TiO2 to synthesize Er3+-rGO/TiO2 photocatalyst through a two-step sol–gel method. Based on the UV–visible diffuse reflectance spectra and photoluminescence spectrum, the introduction of Er3+ and rGO increased the visible light absorption efficiency and enhanced the migration of photogenerated electron. Pure TiO2 has almost no photocatalytic activity for arsanilic acid (p-ASA) degradation under visible light irradiation. However, while doping with 2.0 mol% Er3+ and 10.0 mol% rGO, the p-ASA could be completely degraded within 50 min by the Er3+-rGO/TiO2 photocatalyst under visible light irradiation, and most of produced inorganic arsenic was in situ removed by adsorption from the solution. The reactive oxygen species (ROS) reacting with p-ASA was determined and superoxide radical (O2•−) and singlet oxygen (1O2) were the dominant ROS for the oxidation of p-ASA and arsenite. This work provides an approach of introducing Er3+ and rGO to enhance the visible light photocatalytic efficiency of TiO2. [ABSTRACT FROM AUTHOR]

Published
2023
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