17 results on '"Yuan, Xiangfen"'
Search Results
2. Species discrimination in the subfamily Ostertagiinae of Northern China: assessment of DNA barcode in a taxonomically challenging group
- Author
-
Lv, Jizhou, Zhang, Yongning, Feng, Chunyan, Yuan, Xiangfen, Sun, Degang, Deng, Junhua, Wang, Caixia, Wu, Shaoqiang, and Lin, Xiangmei
- Published
- 2016
- Full Text
- View/download PDF
3. A Novel, Reverse Transcription, Droplet Digital PCR Assay for the Combined, Sensitive Detection of Severe Acute Respiratory Syndrome Coronavirus 2 with Swine Acute Diarrhea Syndrome Coronavirus
- Author
-
Zhang, Zhou, primary, Wang, Na, additional, Liu, Xiaofei, additional, Lv, Jizhou, additional, Jing, Hongli, additional, Yuan, Xiangfen, additional, Chen, Dongjie, additional, Lin, Xiangmei, additional, and Wu, Shaoqiang, additional
- Published
- 2022
- Full Text
- View/download PDF
4. Identification of tick-borne pathogens using metagenomic analyses in H. longicornis feeding on humans in downtown Beijing
- Author
-
Jizhou Lv, Xiangmei Lin, Zhen F. Fu, Wu Shaoqiang, Kong Yufang, Deng Junhua, Lin Mei, Huiyu Wang, Yuan Xiangfen, and Xueqing Han
- Subjects
Tick-borne pathogens ,biology ,Transmission (medicine) ,Host (biology) ,Veterinary medicine ,Cytochrome c oxidase subunit I ,Metagenomic analyses ,Tick ,bacterial infections and mycoses ,biology.organism_classification ,Blood meal ,DNA sequencing ,Microbiology ,Metagenomics ,SF600-1100 ,parasitic diseases ,H. longicornis ,Public aspects of medicine ,RA1-1270 ,Downtown Beijing ,Bacteria - Abstract
On August 14th, 2018, a Beijing resident living in Xicheng District found a female H. longicornis tick attached to the skin at the front of his upper shin. On examination, the patient was afebrile and appeared well. The species of the tick was identified through morphological characteristics and phylogenetic analysis based on cytochrome C oxidase subunit I. This H. longicornis tick was screened for tick-borne pathogens such as viruses, bacteria and parasites. RNA pathogens were screened by PCR and sequencing, while DNA pathogens were screened by metagenomic analyses. It was found that the tick was positive for the DNA sequences of zoonotic and animal pathogens such as A. phagocytophilum, Ehrlichia minasensis and C. burnetii. Considering the good health condition of the patient, we hypothesized that the pathogens originated from the tick specimen itself rather than host blood meal. For the first time, our study reveals the possible risk of transmission of tick-borne pathogens to human beings through tick bit in downtown Beijing. Further research is needed to screen for tick-borne pathogens among unfed ticks collected from central Beijing.
- Published
- 2021
5. Multiplex detection of six swine viruses on an integrated centrifugal disk using loop-mediated isothermal amplification
- Author
-
Deng Junhua, Chunyan Zhang, Wu Shaoqiang, Xiangmei Lin, Yuan Xiangfen, Yonggui Wang, Fan Xiaopan, Jizhou Lv, Caixia Wang, and Xu Hui
- Subjects
Porcine parvovirus ,Materials science ,General Veterinary ,biology ,Swine ,viruses ,Loop-mediated isothermal amplification ,biology.organism_classification ,Polymerase Chain Reaction ,Sensitivity and Specificity ,Virology ,Rapid detection ,Limit of Detection ,Viruses ,Animals ,Multiplex ,Full Scientific Reports ,Foot-and-mouth disease virus ,Nucleic Acid Amplification Techniques - Abstract
Advances in molecular testing and microfluidic technologies have opened new avenues for rapid detection of animal viruses. We used a centrifugal microfluidic disk (CMFD) to detect 6 important swine viruses, including foot-and-mouth disease virus, classical swine fever virus, porcine reproductive and respiratory swine virus–North American genotype, porcine circovirus 2, pseudorabies virus, and porcine parvovirus. Through integrating the loop-mediated isothermal amplification (LAMP) method and microfluidic chip technology, the CMFD could be successfully performed at 62℃ in 60 min. The detection limit of the CMFD was 3.2 × 102 copies per reaction, close to the sensitivity of tube-type LAMP turbidity methods (1 × 102 copies per reaction). In addition, the CMFD was highly specific in detecting the targeted viruses with no cross-reaction with other viruses, including porcine epidemic diarrhea virus, transmissible gastroenteritis virus, and porcine rotavirus. The coincidence rate of CMFD and conventional PCR was ~94%; the CMFD was more sensitive than conventional PCR for detecting mixed viral infections. The positive detection rate of 6 viruses in clinical samples by CMFD was 44.0% (102 of 232), whereas PCR was 40.1% (93 of 232). Thirty-six clinical samples were determined to be coinfected with 2 or more viruses. CMFD can be used for rapid, sensitive, and accurate detection of 6 swine viruses, offering a reliable assay for monitoring these pathogens, especially for detecting viruses in widespread mixed-infection clinical samples.
- Published
- 2019
6. A survey of argasid ticks and tick-associated pathogens in the Peripheral Oases around Tarim Basin and the first record of Argas japonicus in Xinjiang, China
- Author
-
Bo He, Wu Shaoqiang, Jiao-Jiao Pan, Li Zhao, Xiangmei Lin, Kai-Rui Li, Nicholas Johnson, Jia-Min Gao, Fei Li, Jizhou Lv, Qiang-Rong Wang, Yuan Xiangfen, Lu-Yao Zhang, and Yong-Hong Liu
- Subjects
0301 basic medicine ,Rickettsiales ,Argas ,Disease Vectors ,Pathology and Laboratory Medicine ,Biochemistry ,0302 clinical medicine ,Ticks ,RNA, Ribosomal, 16S ,Medicine and Health Sciences ,Acari ,Rickettsia ,Phylogeny ,education.field_of_study ,Multidisciplinary ,biology ,Database and informatics methods ,Argasidae ,Sequence analysis ,Eukaryota ,Agriculture ,Bacterial Pathogens ,Mitochondria ,Nucleic acids ,Infectious Diseases ,Ribosomal RNA ,Medical Microbiology ,Medicine ,Pathogens ,Ixodidae ,Research Article ,Cell biology ,China ,Livestock ,Cellular structures and organelles ,Anaplasma ,Arthropoda ,Bioinformatics ,Science ,030231 tropical medicine ,Population ,Zoology ,Tick ,Microbiology ,03 medical and health sciences ,Arachnida ,parasitic diseases ,Animals ,education ,Non-coding RNA ,Ornithodoros ,Microbial Pathogens ,DNA sequence analysis ,Sheep ,Ixodes ,Bacteria ,Organisms ,Biology and Life Sciences ,Sequence Analysis, DNA ,biology.organism_classification ,bacterial infections and mycoses ,Invertebrates ,Spotted fever ,Tick Infestations ,Research and analysis methods ,Species Interactions ,030104 developmental biology ,RNA, Ribosomal ,Vector (epidemiology) ,RNA ,Cattle ,Ribosomes - Abstract
Argasid ticks (Acari: Argasidae) carry and transmit a variety of pathogens of animals and humans, including viruses, bacteria and parasites. There are several studies reporting ixodid ticks (Acari: Ixodidae) and associated tick-borne pathogens in Xinjiang, China. However, little is known about the argasid ticks and argasid tick-associated pathogens in this area. In this study, a total of 3829 adult argasid ticks infesting livestock were collected at 12 sampling sites of 10 counties in the Peripheral Oases, which carry 90% of the livestock and humans population, around the Tarim Basin (southern Xinjiang) from 2013 to 2016. Tick specimens were identified to two species from different genera by morphology and sequences of mitochondrial 16S rRNA and 12S rRNA were derived to confirm the species designation. The results showed that the dominant argasid ticks infesting livestock in southern Xinjiang were Ornithodoros lahorensis (87.86%, 3364/3829). Ornithodoros lahorensis was distributed widely and were collected from 10 counties of southern Xinjiang. Argas japonicus was collected from Xinjiang for the first time. In addition, we screened these ticks for tick-associated pathogens and showed the presence of DNA sequences of Rickettsia spp. of Spotted fever group and Anaplasma spp. in the argasid ticks. This finding suggests the potential role for Argas japonicus as a vector of pathogens to livestock and humans.
- Published
- 2018
7. Multiplex detection of six swine viruses on an integrated centrifugal disk using loop-mediated isothermal amplification
- Author
-
Yuan, Xiangfen, primary, Lv, Jizhou, additional, Lin, Xiangmei, additional, Zhang, Chunyan, additional, Deng, Junhua, additional, Wang, Caixia, additional, Fan, Xiaopan, additional, Wang, Yonggui, additional, Xu, Hui, additional, and Wu, Shaoqiang, additional
- Published
- 2019
- Full Text
- View/download PDF
8. Expression and purification of the nucleocapsid protein of Schmallenberg virus, and preparation and characterization of a monoclonal antibody against this protein
- Author
-
Deng Junhua, Jihong Zhang, Yuan Xiangfen, Xiangmei Lin, Caixia Wang, Jianchang Wang, Chunyan Feng, Jizhou Lv, Kerstin Wernike, Wu Shaoqiang, and Yongning Zhang
- Subjects
Orthobunyavirus ,medicine.drug_class ,Bunyaviridae Infections ,Monoclonal antibody ,Epitope ,Cell Line ,law.invention ,Mice ,Western blot ,Affinity chromatography ,Antibody Specificity ,law ,medicine ,Animals ,Cloning, Molecular ,Mice, Inbred BALB C ,Sheep ,biology ,medicine.diagnostic_test ,Goats ,Antibodies, Monoclonal ,Schmallenberg virus ,Nucleocapsid Proteins ,biology.organism_classification ,Fusion protein ,Virology ,Molecular biology ,Recombinant Proteins ,Recombinant DNA ,Cattle ,Female ,Biotechnology - Abstract
Schmallenberg virus (SBV) is a novel orthobunyavirus that primarily infects ruminants such as cattle, sheep and goats. The nucleocapsid (N) protein of SBV has been shown to be an ideal target antigen for serological detection. To prepare a monoclonal antibody (mAb) against the N protein, the full-length coding sequence of the SBV N gene was cloned into pET-28a-c(+) and pMAL-c5X vectors to generate two recombinant plasmids, which were expressed in Escherichia coli BL21 as histidine (His)-tagged (His-SBV-N) and maltose-binding protein (MBP)-tagged (MBP-SBV-N) fusion proteins, respectively. After affinity purification of His-SBV-N with Ni-NTA agarose and MBP-SBV-N with amylose resin, His-SBV-N was used to immunize BALB/c mice, while MBP-SBV-N was utilized to screen for mAb-secreting hybridomas. Six hybridoma cell lines stably secreting mAbs against N were obtained. Clone 2C8 was selected for further study because of its rapid growth characteristics in vitro and good reactivity with recombinant SBV N proteins in enzyme-linked immunosorbent assays. The epitope recognized by 2C8 is located at amino acids 51-76 of the SBV N protein. Western blot analyses showed that 2C8 reacts with both recombinant SBV N proteins and SBV isolates. It is also cross-reactive with the N proteins of genetically related Shamonda, Douglas and Akabane viruses, but not with the Rift Valley fever virus N protein. The successful preparation of recombinant N proteins and mAbs provides valuable materials that can be used in the serological diagnosis of SBV.
- Published
- 2013
9. Peste des petits ruminants virus exploits cellular autophagy machinery for replication
- Author
-
Deng Junhua, Tianyi Zhang, Jizhou Lv, Yuan Xiangfen, Yongning Zhang, Xiangmei Lin, Chunyan Feng, Wu Shaoqiang, and Caixia Wang
- Subjects
Sirolimus ,Autophagy ,RNA ,Ubiquitin-Activating Enzymes ,Biology ,Nucleocapsid Proteins ,Virus Replication ,Virology ,Peste-des-petits-ruminants virus ,Antigen ,Apoptosis ,RNA interference ,Chlorocebus aethiops ,Vero cell ,Animals ,RNA Interference ,RNA, Small Interfering ,Pathogen ,Antigens, Viral ,Microtubule-Associated Proteins ,Vero Cells - Abstract
Peste des petits ruminants virus (PPRV) is an important pathogen that seriously influences the productivity of small ruminants worldwide. Although PPRV is known to induce apoptosis in infected cells, the interaction between PPRV and permissive cells requires further elucidation. Here, we provide the first evidence that PPRV infection triggered autophagy in Vero cells based on the appearance of abundant double- and single-membrane vesicles, the accumulation of LC3 fluorescent puncta, the enhancement of LC3-I/-II conversion, and autophagic flux. We further demonstrated that induction of autophagy with rapamycin significantly increased PPRV progeny yield and nucleocapsid (N) protein expression, while inhibition of autophagy with siRNA targeting ATG7 resulted in diametrically opposite results. Our data indicate that PPRV exploits the autophagy machinery to facilitate its own replication in host cells, thus the production efficiency of live attenuated PPRV vaccines may be improved by targeting the autophagic pathway.
- Published
- 2013
- Full Text
- View/download PDF
10. Species discrimination in the subfamily Ostertagiinae of Northern China: assessment of DNA barcode in a taxonomically challenging group
- Author
-
Chunyan Feng, Xiangmei Lin, Deng Junhua, Yuan Xiangfen, Caixia Wang, Degang Sun, Jizhou Lv, Yongning Zhang, and Wu Shaoqiang
- Subjects
0301 basic medicine ,Mitochondrial DNA ,China ,Lineage (evolution) ,Zoology ,Cattle Diseases ,Sheep Diseases ,Biology ,DNA barcoding ,DNA, Mitochondrial ,Intraspecific competition ,Trichostrongyloidiasis ,03 medical and health sciences ,parasitic diseases ,Animals ,DNA Barcoding, Taxonomic ,Sheep ,General Veterinary ,Trichostrongyloidea ,Cytochrome c oxidase subunit I ,Ostertagia ,General Medicine ,DNA, Helminth ,Teladorsagia circumcincta ,Genetic divergence ,030104 developmental biology ,Infectious Diseases ,Insect Science ,Parasitology ,Cattle - Abstract
Gastrointestinal nematodes within the subfamily Ostertagiinae (Teladorsagia, Ostertagia, and Marshallagia et al.) are among the most common infections of domesticated livestock. These parasites are of particular interest, as many of the species within this group are of economic importance worldwide. Traditionally, nematode species designations have been based on morphological criteria. However, this group possesses poorly defined species. There is an urgent need to develop a reliable technique that can distinguish species of Ostertagiinae. DNA barcoding has been proved to be a powerful tool to identify species of birds, mammals, and arthropods, but this technique has not yet been examined for identifying species of Ostertagiinae. In this study, a total of 138 mitochondrial DNA (mtDNA) cytochrome c oxidase subunit I (COI) sequences from individuals representing 11 species of Ostertagiinae were acquired by PCR for the first time. The specimens were collected from pastoral area of northern China. Genetic divergence analyses showed that mean interspecific Kimura two-parameter distances of COI (13.61 %) were about four times higher than the mean value of the intraspecific divergence (3.69 %). Then, the performance of the COI to identify species of Ostertagiinae was evaluated by identification success rates using nearest neighbor (NN) and BLASTn. The results indicated that the rates of correct sequence identification for COI were high (>80 %) when using the NN and BLASTn methods. Besides, the deep lineage divergences are detected in Teladorsagia circumcincta. Meanwhile, the analyses also detected no genetic differentiation between some species such as Ostertagia hahurica and Ostertagia buriatica. These results indicate that the traditional status of species within Ostertagiinae should be closely examined based on the molecular data.
- Published
- 2015
11. Assessment of four DNA fragments (COI, 16S rDNA, ITS2, 12S rDNA) for species identification of the Ixodida (Acari: Ixodida)
- Author
-
Xiangmei Lin, Lin Mei, Guangle Jia, Deng Junhua, Caixia Wang, Wang Qin, Yuan Xiangfen, Wu Shaoqiang, Yongning Zhang, Chunyan Feng, Yan Chen, and Jizhou Lv
- Subjects
12S rDNA ,Genetic Markers ,Sequence analysis ,Molecular Sequence Data ,ITS2 ,Zoology ,macromolecular substances ,Biology ,DNA, Ribosomal ,Polymerase Chain Reaction ,DNA barcoding ,law.invention ,COI ,Electron Transport Complex IV ,Ticks ,Species Specificity ,Phylogenetics ,law ,16S rDNA ,DNA, Ribosomal Spacer ,parasitic diseases ,Genetic variation ,Nearest neighbour ,Animals ,DNA Barcoding, Taxonomic ,Coding region ,BLASTn ,Species identification ,Phylogeny ,Polymerase chain reaction ,DNA Primers ,Base Sequence ,Research ,fungi ,Genetic Variation ,Sequence Analysis, DNA ,16S ribosomal RNA ,Infectious Diseases ,Genetic marker ,Evolutionary biology ,Parasitology ,DNA Barcode ,Sequence Alignment - Abstract
Background The 5’ region of cytochrome oxidase I (COI) is the standard marker for DNA barcoding. However, COI has proved to be of limited use in identifying some species, and for some taxa, the coding sequence is not efficiently amplified by PCR. These deficiencies lead to uncertainty as to whether COI is the most suitable barcoding fragment for species identification of ticks. Methods In this study, we directly compared the relative effectiveness of COI, 16S ribosomal DNA (rDNA), nuclear ribosomal internal transcribed spacer 2 (ITS2) and 12S rDNA for tick species identification. A total of 307 sequences from 84 specimens representing eight tick species were acquired by PCR. Besides the 1,834 published sequences of 189 tick species from GenBank and the Barcode of Life Database, 430 unpublished sequences representing 59 tick species were also successfully screened by Bayesian analyses. Thereafter, the performance of the four DNA markers to identify tick species was evaluated by identification success rates given by these markers using nearest neighbour (NN), BLASTn, liberal tree-based or liberal tree-based (+threshold) methods. Results Genetic divergence analyses showed that the intra-specific divergence of each marker was much lower than the inter-specific divergence. Our results indicated that the rates of correct sequence identification for all four markers (COI, 16S rDNA, ITS2, 12S rDNA) were very high (> 96%) when using the NN methodology. We also found that COI was not significantly better than the other markers in terms of its rate of correct sequence identification. Overall, BLASTn and NN methods produced higher rates of correct species identification than that produced by the liberal tree-based methods (+threshold or otherwise). Conclusions As the standard DNA barcode, COI should be the first choice for tick species identification, while 16S rDNA, ITS2, and 12S rDNA could be used when COI does not produce reliable results. Besides, NN and BLASTn are efficient methods for species identification of ticks.
- Published
- 2014
12. Species discrimination in the subfamily Ostertagiinae of Northern China: assessment of DNA barcode in a taxonomically challenging group
- Author
-
Lv, Jizhou, primary, Zhang, Yongning, additional, Feng, Chunyan, additional, Yuan, Xiangfen, additional, Sun, Degang, additional, Deng, Junhua, additional, Wang, Caixia, additional, Wu, Shaoqiang, additional, and Lin, Xiangmei, additional
- Published
- 2015
- Full Text
- View/download PDF
13. Development of a loop-mediated isothermal amplification method for detection of Perkinsus spp. in mollusks
- Author
-
Jizhou Lv, Lin Mei, Deng Junhua, Xiangmei Lin, Yongning Zhang, Chunyan Feng, Caixia Wang, Wu Shaoqiang, and Yuan Xiangfen
- Subjects
Detection limit ,biology ,Ecology ,Loop-mediated isothermal amplification ,Aquatic Science ,Ribosomal RNA ,biology.organism_classification ,Rapid detection ,Polymerase Chain Reaction ,Sensitivity and Specificity ,law.invention ,Microbiology ,Bivalvia ,Host-Parasite Interactions ,Mass mortality ,law ,Alveolata ,Recombinant DNA ,Animals ,Perkinsus ,Internal transcribed spacer ,Nucleic Acid Amplification Techniques ,Ecology, Evolution, Behavior and Systematics - Abstract
Perkinsus is a genus of unicellular protozoan parasite responsible for mass mortality of several commercially valuable mollusks. Surveillance and inspection of its epidemiology in the field calls for convenient and rapid detection methods. Here, a loop-mediated isothermal amplifi- cation (LAMP) assay was developed to detect the presence of Perkinsus spp. in mollusks. Specific LAMP primers were designed targeting the conserved internal transcribed spacer 2 (ITS-2) region of the rRNA gene of Perkinsus spp. Using ITS-2 recombinant plasmid as a template, we optimized the LAMP reaction system and conditions and then evaluated the analytical sensitivity and speci- ficity of the assay. The LAMP assay was validated using clam samples collected from coastal areas in eastern China and oysters imported to China and compared with the traditional Ray's fluid thio- glycollate culture method (RFTM). Our results showed that the LAMP detection method for Perkinsus was successful. The detection limit was 10 copies of plasmid DNA. Compared to the RFTM assay, the LAMP detection method was more sensitive (56 versus 52 positive out of 60 sam- ples). P. olseni and P. marinus from infected hosts were successfully detected by this method. The LAMP method is rapid, sensitive, and specific for Perkinsus spp. detection, and could be used to screen for perkinsosis both on farms and at ports.
- Published
- 2013
14. Assessment of four DNA fragments (COI, 16S rDNA, ITS2, 12S rDNA) for species identification of the Ixodida (Acari: Ixodida)
- Author
-
Lv, Jizhou, primary, Wu, Shaoqiang, additional, Zhang, Yongning, additional, Chen, Yan, additional, Feng, Chunyan, additional, Yuan, Xiangfen, additional, Jia, Guangle, additional, Deng, Junhua, additional, Wang, Caixia, additional, Wang, Qin, additional, Mei, Lin, additional, and Lin, Xiangmei, additional
- Published
- 2014
- Full Text
- View/download PDF
15. Expression and purification of the nucleocapsid protein of Schmallenberg virus, and preparation and characterization of a monoclonal antibody against this protein
- Author
-
Zhang, Yongning, primary, Wu, Shaoqiang, additional, Wang, Jianchang, additional, Wernike, Kerstin, additional, Lv, Jizhou, additional, Feng, Chunyan, additional, Zhang, Jihong, additional, Wang, Caixia, additional, Deng, Junhua, additional, Yuan, Xiangfen, additional, and Lin, Xiangmei, additional
- Published
- 2013
- Full Text
- View/download PDF
16. Peste des petits ruminants virus exploits cellular autophagy machinery for replication
- Author
-
Zhang, Yongning, primary, Wu, Shaoqiang, additional, Lv, Jizhou, additional, Feng, Chunyan, additional, Deng, Junhua, additional, Wang, Caixia, additional, Yuan, Xiangfen, additional, Zhang, Tianyi, additional, and Lin, Xiangmei, additional
- Published
- 2013
- Full Text
- View/download PDF
17. Development of a loop-mediated isothermal amplification method for detection of Perkinsus spp. in mollusks.
- Author
-
Feng C, Wang C, Lin X, Zhang Y, Lv J, Deng J, Yuan X, Mei L, and Wu S
- Subjects
- Alveolata genetics, Animals, Host-Parasite Interactions, Polymerase Chain Reaction, Sensitivity and Specificity, Alveolata isolation & purification, Bivalvia parasitology, Nucleic Acid Amplification Techniques methods
- Abstract
Perkinsus is a genus of unicellular protozoan parasite responsible for mass mortality of several commercially valuable mollusks. Surveillance and inspection of its epidemiology in the field calls for convenient and rapid detection methods. Here, a loop-mediated isothermal amplification (LAMP) assay was developed to detect the presence of Perkinsus spp. in mollusks. Specific LAMP primers were designed targeting the conserved internal transcribed spacer 2 (ITS-2) region of the rRNA gene of Perkinsus spp. Using ITS-2 recombinant plasmid as a template, we optimized the LAMP reaction system and conditions and then evaluated the analytical sensitivity and specificity of the assay. The LAMP assay was validated using clam samples collected from coastal areas in eastern China and oysters imported to China and compared with the traditional Ray's fluid thioglycollate culture method (RFTM). Our results showed that the LAMP detection method for Perkinsus was successful. The detection limit was 10 copies of plasmid DNA. Compared to the RFTM assay, the LAMP detection method was more sensitive (56 versus 52 positive out of 60 samples). P. olseni and P. marinus from infected hosts were successfully detected by this method. The LAMP method is rapid, sensitive, and specific for Perkinsus spp. detection, and could be used to screen for perkinsosis both on farms and at ports.
- Published
- 2013
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.