Search

Your search keyword '"Yuan, Ying-Jin"' showing total 1,096 results
Start Over Author "Yuan, Ying-Jin"
1,096 results on '"Yuan, Ying-Jin"'

35. Highly efficient synthesis of lysergic acid using engineered budding yeast.

Author

Wu, Nan, Yao, Mingdong, Xiao, Wenhai, Wang, Ying, and Yuan, Ying-Jin

Abstract

Lysergic acid (LA) is the basic precursor for the biosynthesis of various ergot alkaloids of pharmaceutical importance. The heterologous biosynthesis of LA in microbes is a promising method to reduce industrial dependence on highly costly and toxic crop-pathogenic ergot fungi. However, the biosynthetic efficiency of LA remains unsatisfactory because of the lack of effective pathways and low heterologous production performance. In this work, a microbial host, specifically a Saccharomyces cerevisiae strain, was constructed by applying a metabolic engineering strategy for efficient LA production. The SCH9 knockout was identified as a key factor for enhancing LA yield. Transcriptional analysis revealed that SCH9 deletion significantly enhanced peroxisomal metabolism and cellular translation. Accordingly, tailored approaches were designed to optimize the activities of two rate-limiting enzymes, EasC and CloA, in SCH9 deletion strains. The relocation of EasC to peroxisomes combined with PEX34 overexpression clearly increased the catalytic activity of these enzymes, increasing LA production by 2.31-fold. Moreover, spatial reorientation of the cytochrome P450 CloA and its reductase on the endoplasmic reticulum was performed, which improved electron transfer efficiency, resulting in a 36.8% improvement in LA production. These engineering strategies finally led to a 17.4-fold increase in the LA titre. The final engineered strain produced 509.8 mg L−1 LA under 50 L fed-batch fermentation, yielding the highest reported titre for heterologous hosts. These findings demonstrated a green alternative to the current ergot-based routes, offering a versatile platform for the sustainable, large-scale fermentation of pharmaceutical ergot alkaloids. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

37. The Mediating Effect of Bicultural Self-Efficacy on Acculturation and Career Decision-Making Self-Efficacy for International Students in South Korea

Author

Yuan Ying Jin, Sungsik Ahn, and Sang Min Lee

Subjects
bicultural self-efficacy, acculturation, international student, career development, career counseling, Psychology, BF1-990
Abstract

The population of international students in South Korea is growing. During the career development phase, international students face unique challenges related to their bicultural identity and acculturation experiences. The present study examined the role of bicultural self-efficacy on mediating the relationship between acculturation and career decision-making self-efficacy for international students in South Korea. Responses from 120 international students in South Korea were analyzed using structural equation modeling. The results showed that bicultural self-efficacy fully mediated the relationship between acculturation to mainstream culture and career decision-making self-efficacy but did not mediate the relationship between acculturation to heritage culture and career decision-making self-efficacy. The implications for effective educational intervention for international college students’ career development were discussed based on the results.

Published
2022
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results