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7 results on '"Yukako Maeda"'

1. Children’s Perception of Cancer and Survivors After a Cancer Education Program Engaging with Survivors: A Qualitative Study

Author

Noriko Teruya, Minami Kubagawa, Yukako Maeda, and Yasutaka Kimura

Subjects
health promotion, child, cancer survivors, schools, cancer education, Public aspects of medicine, RA1-1270
Abstract

Background: In Japan, cancer education utilizing external lecturers are being promoted as part of cancer control measures. The objective of this study was to clarify children’s perceptions of cancer and survivors and their awareness of actions for early prevention and detection of cancer after participating in a cancer education program engaging with survivors.Methods: This study was conducted during January and March 2020, using the qualitative analysis method. Homeroom teachers gave a lesson on cancer to sixth-grade students (n=69) at an elementary school in Japan. Two weeks later, a cancer survivor gave a follow-up class. Then an inductive content analysis was conducted on the content of the post-class reflection worksheets written by students.Results: After analyzing the worksheets of 59 students, they learned that “to reduce the risk of developing cancer, one should lead a healthy lifestyle” and “strive for early detection through cancer screening” through the education program. As things they can do themselves in the future, they listed “encourage family members to get cancer screenings,” “quit smoking,” and “cut back on alcohol.” In addition, children have deepened their understanding of cancer survivors and learned the importance of their own and others’ lives.Conclusion: The results suggested that children gained practical knowledge about cancer through the program and deepened their understanding of cancer and survivors by directly interacting with and listening to survivors.

Published
2022
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2. Case Report: A Pediatric Case of Familial Mediterranean Fever Concurrent With Autoimmune Hepatitis

Author

Mariko Aoki, Kazushi Izawa, Takayuki Tanaka, Yoshitaka Honda, Takeshi Shiba, Yukako Maeda, Takayuki Miyamoto, Keisuke Okamoto, Masahiko Nishitani-Isa, Hiroshi Nihira, Kohsuke Imai, Junko Takita, Ryuta Nishikomori, Eitaro Hiejima, and Takahiro Yasumi

Subjects
familial Mediterranean fever (FMF), autoimmune hepatitis (AIH), liver injury, functional analysis, pediatric case report, Immunologic diseases. Allergy, RC581-607
Abstract

Familial Mediterranean fever (FMF) is a hereditary, autoinflammatory disease that causes recurrent fever, arthritis, and serositis. The diagnosis of FMF is based on the presentation of typical clinical symptoms and the Mediterranean fever gene (MEFV) test. However, the challenge lies in diagnosing atypical cases. In this report, we have described a pediatric patient with complex FMF whose diagnosis required trio-whole exome sequencing (WES) and functional validation of a rare MEFV variant. A 3-year-old boy presented with recurrent episodes of elevated liver enzymes and arthralgia. He was diagnosed with autoimmune hepatitis (AIH), and his liver enzymes improved rapidly with steroid treatment. However, he exhibited recurrent arthralgia and severe abdominal attacks. Trio-WES identified compound heterozygous mutations in MEFV (V726A and I692del). Ex vivo functional assays of the patient’s monocytes and macrophages, which had been pre-treated with Clostridium difficile toxin A (TcdA) and colchicine, were comparable to those of typical FMF patients, thereby confirming the diagnosis of FMF. Although he was intolerant to colchicine because of liver toxicity, subsequent administration of canakinumab successfully ameliorated his abdominal attacks. However, it was ineffective against liver injury, which recurred after steroid tapering. Therefore, in this case, the pathogenesis of AIH was probably interleukin-1β (IL-1β)-independent. In fact, AIH might have been a concurrent disease with FMF, rather than being one of its complications. Nevertheless, further studies are necessary to determine whether FMF-induced inflammasome activation contributes to AIH development. Moreover, we must consider the possibility of mixed phenotypes in such atypical patients who present distinct pathologies simultaneously.

Published
2022
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3. Rapid Flow Cytometry-Based Assay for the Functional Classification of MEFV Variants

Author

Hiroki Mukoyama, Junko Takita, Takayuki Miyamoto, Takayuki Tanaka, Osamu Ohara, Yoshitaka Honda, Haruna Nakaseko, Takeshi Shiba, Keisuke Nishimura, Masahiko Isa-Nishitani, Hirofumi Shibata, Eitaro Hiejima, Yukako Maeda, Kazushi Izawa, Hiroshi Nihira, Takahiro Yasumi, and Ryuta Nishikomori

Subjects
0301 basic medicine, medicine.diagnostic_test, Immunology, Cell, Clostridium difficile toxin A, Familial Mediterranean fever, Inflammasome, Biology, MEFV, medicine.disease, Phenotype, Pyrin domain, Flow cytometry, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, medicine.anatomical_structure, medicine, Immunology and Allergy, 030215 immunology, medicine.drug
Abstract

Pathogenic MEFV variants cause pyrin-associated autoinflammatory diseases (PAADs), which include familial Mediterranean fever (FMF), FMF-like disease, and pyrin-associated autoinflammation with neutrophilic dermatosis (PAAND). The diagnosis of PAADs is established by clinical phenotypic and genetic analyses. However, the pathogenicity of most MEFV variants remains controversial, as they have not been functionally evaluated. This study aimed to establish and validate a new functional assay to evaluate the pathogenicity of MEFV variants. We transfected THP-1 monocytes with 32 MEFV variants and analyzed their effects on cell death with or without stimulation with Clostridium difficile toxin A (TcdA) or UCN-01. These variants were classified using hierarchical cluster analysis. Macrophages were obtained from three healthy controls and two patients with a novel homozygous MEFVP257L variant, for comparison of IL-1β secretion using a cell-based assay and a novel THP-1-based assay. Disease-associated MEFV variants induced variable degrees of spontaneous or TcdA/UCN-01-induced cell death in THP-1. Cell death was caspase-1 dependent and was accompanied by ASC speck formation and IL-1β secretion, indicating that pathogenic MEFV variants induced abnormal pyrin inflammasome activation and subsequent pyroptotic cell deaths in this assay. The MEFV variants (n = 32) exhibiting distinct response signatures were classified into 6 clusters, which showed a good correlation with the clinical phenotypes. Regarding the pathogenicity of MEFVP257L variants, the results were consistent between the cell-based assay and the THP-1-based assay. Our assay facilitates a rapid and comprehensive assessment of the pathogenicity of MEFV variants and contributes to a refined definition of PAAD subtypes.

Published
2021

4. Rapid Flow Cytometry-Based Assay for the Functional Classification of MEFV Variants

Author

Yoshitaka, Honda, Yukako, Maeda, Kazushi, Izawa, Takeshi, Shiba, Takayuki, Tanaka, Haruna, Nakaseko, Keisuke, Nishimura, Hiroki, Mukoyama, Masahiko, Isa-Nishitani, Takayuki, Miyamoto, Hiroshi, Nihira, Hirofumi, Shibata, Eitaro, Hiejima, Osamu, Ohara, Junko, Takita, Takahiro, Yasumi, and Ryuta, Nishikomori

Subjects
Male, Cell Death, Inflammasomes, THP-1 Cells, Genetic Variation, Middle Aged, Pyrin, Flow Cytometry, Monocytes, Cell Line, Phenotype, Humans, Female, Genetic Predisposition to Disease
Abstract

Pathogenic MEFV variants cause pyrin-associated autoinflammatory diseases (PAADs), which include familial Mediterranean fever (FMF), FMF-like disease, and pyrin-associated autoinflammation with neutrophilic dermatosis (PAAND). The diagnosis of PAADs is established by clinical phenotypic and genetic analyses. However, the pathogenicity of most MEFV variants remains controversial, as they have not been functionally evaluated. This study aimed to establish and validate a new functional assay to evaluate the pathogenicity of MEFV variants.We transfected THP-1 monocytes with 32 MEFV variants and analyzed their effects on cell death with or without stimulation with Clostridium difficile toxin A (TcdA) or UCN-01. These variants were classified using hierarchical cluster analysis. Macrophages were obtained from three healthy controls and two patients with a novel homozygous MEFVDisease-associated MEFV variants induced variable degrees of spontaneous or TcdA/UCN-01-induced cell death in THP-1. Cell death was caspase-1 dependent and was accompanied by ASC speck formation and IL-1β secretion, indicating that pathogenic MEFV variants induced abnormal pyrin inflammasome activation and subsequent pyroptotic cell deaths in this assay. The MEFV variants (n = 32) exhibiting distinct response signatures were classified into 6 clusters, which showed a good correlation with the clinical phenotypes. Regarding the pathogenicity of MEFVOur assay facilitates a rapid and comprehensive assessment of the pathogenicity of MEFV variants and contributes to a refined definition of PAAD subtypes.

Published
2020

5. A novel NLRP3 variant in two unrelated patients with cryopyrin-associated periodic syndrome

Author

Takahiro Yasumi, Ryuta Nishikomori, Masahiko Nishitani, Tomoki Kawai, Takayuki Tanaka, Hiroshi Nihira, Yoshitaka Honda, Toshio Heike, Takeshi Shiba, Yukako Maeda, Kazushi Izawa, Naomi Iwata, Yoko Okada, Haruna Nakaseko, Shintaro Ono, and Eitaro Hiejima

Subjects
medicine.medical_specialty, Periodic syndrome, business.industry, Urticarial rash, medicine, Cryopyrin-associated periodic syndrome, Autoinflammatory Syndrome, medicine.disease, business, Dermatology
Abstract

Cryopyrin-associated periodic syndrome (CAPS) is an autoinflammatory syndrome characterised by recurrent episodes of urticarial rash and fever. Diagnosis is based on characteristic clinical...

Published
2017

6. Mantle cell lymphoma presenting with spontaneous splenic rupture

Author

Yukako, Maeda-Sakagami, Yasuhiro, Tanaka, Yusuke, Koba, Isaku, Shinzato, and Takayuki, Ishikawa

Subjects
Male, Peripheral Blood Stem Cell Transplantation, Treatment Outcome, Rupture, Spontaneous, Positron Emission Tomography Computed Tomography, Antineoplastic Combined Chemotherapy Protocols, Humans, Lymphoma, Mantle-Cell, Splenic Rupture, Middle Aged, Transplantation, Autologous
Abstract

A 48-year-old man was transferred to our emergency room because of sudden-onset epigastric pain and nausea. Abdominal contrast-enhanced computed tomography (CT) showed splenomegaly with splenic infarction and intra-abdominal bleeding, suggestive of splenic rupture. An emergent open splenectomy was performed. His spleen was markedly swollen and showed continuous bleeding due to a laceration. On histopathological examination, his spleen was filled with abnormal tumor cells. He was diagnosed as having mantle cell lymphoma based on the findings of immunohistochemical and cytogenetic analyses of the spleen. Mantle cell lymphoma cells were identified in the bone marrow and ileum, and he was determined to be in stageIVA by positron emission tomography (PET)-CT. He was administered rituximab combined with hyper-CVAD/MA chemotherapy (R-hyper-CVAD/MA regimen). After two courses of the R-hyper-CVAD/MA regimen, he achieved complete response, as confirmed by PET-CT. He received four courses in total of the R-hyper-CVAD/MA regimen, followed sequentially by high-dose chemotherapy and autologous peripheral blood stem cell transplantation (auto-PBSCT). He is currently alive and free of disease. This is the 10(th) report of a mantle cell lymphoma case with spontaneous splenic rupture. We herein review previous reports and emphasize the importance of awareness of hematological malignancies when encountering a case with spontaneous splenic rupture.

Published
2016

7. A new in vitro system for evaluation of passive intestinal drug absorption: establishment of a double artificial membrane permeation assay

Author

Yukako Maeda, Saki Tsuneishi, Shinji Yamashita, Yoshie Masaoka, Shinji Sakuma, and Makoto Kataoka

Subjects
Intestinal permeability, Chromatography, Chemistry, Synthetic membrane, Pharmaceutical Science, Membranes, Artificial, General Medicine, Permeation, medicine.disease, Epithelium, Permeability, chemistry.chemical_compound, medicine.anatomical_structure, Intestinal Absorption, Pharmaceutical Preparations, Phosphatidylcholine, Paracellular transport, medicine, Humans, Transcellular, Caco-2 Cells, Intracellular, Biotechnology
Abstract

The aim of this present study was to establish a new in vitro assay, double artificial membrane permeation assay (DAMPA), to evaluate the human intestinal permeability of drugs. A double artificial membrane with an intracellular compartment was constructed in side-by-side chambers by sandwiching a filter containing buffer solution with impregnated lipophilic filters with dodecane containing 2 w/v% phosphatidylcholine. Permeation data of ionic compounds clearly indicated that not only the pH value of the apical solution but also that of the intracellular compartment affected the permeability across the double artificial membrane. DAMPA was performed with 20 compounds at physiological pH (apical; 6.5, intracellular and basal; 7.4). Paracellular and transcellular permeabilities of compounds in human epithelium were estimated based on the characteristics of the paracellular pathway using physicochemical properties of compounds with the Renkin function and the area factor i.e. the difference in the effective surface area between human epithelium and the double artificial membrane, respectively. The human intestinal permeability of each compound was predicted by the sum of estimated transcellular and paracellular permeabilities. Predicted human intestinal permeability was significantly correlated with the fraction of absorbed dose in humans, indicating that DAMPA has the potential to predict oral absorption of drugs in humans.

Published
2014

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