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The Japan Meteorological Agency (JMA) has developed the third Japanese global atmospheric reanalysis, the Japanese Reanalysis for Three Quarters of a Century (JRA-3Q). The objective of JRA-3Q is to improve quality in terms of issues identified in the previous Japanese 55-year Reanalysis (JRA-55) and to extend the reanalysis period further into the past. JRA-3Q is based on the TL479 version of the JMA global Numerical Weather Prediction (NWP) system as of December 2018 and uses results of developments in the operational NWP system, boundary conditions, and forcing fields achieved at JMA since JRA-55. It covers the period from September 1947, when Typhoon Kathleen brought severe flood damage to Japan, and uses rescued historical observations to extend its analyses backwards in time about 10 years earlier than JRA-55. This paper describes the data assimilation system, forecast model, observations, boundary conditions, and forcing fields used to produce JRA-3Q as well as the basic characteristics of the JRA-3Q product. The initial quality evaluation revealed major improvements from JRA-55 in the global energy budget and representation of tropical cyclones (TCs). One of the major problems in JRA-55--global energy imbalance with excess upward net energy flux at the top of the atmosphere and at the surface--has been significantly reduced in JRA-3Q. Another problem--a trend of artificial weakening of TCs--has been resolved through the use of a method that generates TC bogus based on the JMA operational system. There remain several problems such that the volcanic-induced stratospheric warming is smaller than expected. This paper discusses the causes of such problems and possible solutions in future reanalyses. [ABSTRACT FROM AUTHOR]

The marine dinoflagellates species belonging to the genera Dinophysis and Phalacroma have been reported as mixotrophic or heterotrophic actively feeding on planktonic ciliates. However, limited information is available on the identification of ciliate species preyed on by Phalacroma mitra. An effective method is introduced for detecting prey DNA of dinoflagellates belonging to these two genera. Three cells of P. mitra possessing food vacuoles were isolated from natural seawater, and the prey DNA within the vacuoles was analyzed. After the polymerase chain reaction (PCR)-based amplification, a restriction enzyme specific to the DNA of Dinophysis and Phalacroma was used to concentrate the prey DNA. Gene cloning revealed that the undigested PCR products contained DNA of ciliate species (Euplotes sp., Mesodinium rubrum, Spirostrombidium sp., etc.), as well as that of dinoflagellates, cryptophytes, and radiolarians. These results imply that the prey diversity of P. mitra can be traced. Furthermore, this method can provide useful data to reveal novel insights into food webs in the planktonic ecosystem. [ABSTRACT FROM AUTHOR]

Background and Objectives: In Crohn's disease (CD), belonging to inflammatory bowel disease, the small intestine is involved in most cases. Most frequently affected is the distal ileum, where vitamin B-12 is specifically absorbed. Therefore, malabsorption of vitamin B-12 is quite likely to occur in patients with CD. In this study, we have studied the vitamin B-12 status in CD patients.Methods and Study Design: Forty eight patients with CD were evaluated for their food intake, and circulating concentrations of vitamin B-12, folic acid, and homocysteine (Hcy) as a sensitive marker for the insufficiency of these vitamins and a risk factor of atherosclerosis.Results: Plasma Hcy concentration was significantly correlated with serum vitamin B-12 concentration alone, and 60.4 % of the subjects had hyperhomocysteinemia. Receiver Operating Characteristics (ROC) analysis showed that serum concentration of vitamin B-12, but not folic acid, predicted hyperhomocysteinemia. Their intake of vitamin B-12 was much higher than the Japanese RDA, but not correlated with blood concentrations of vitamin B-12 or Hcy, probably due to malabsorption.Conclusions: Vitamin B-12 insufficiency and hyperhomocysteinemia were highly prevalent in CD patients. Recently, the significance of extra-intestinal complications of CD has been increasingly recognized, and our finding is likely to be of clinical importance. [ABSTRACT FROM AUTHOR]