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Your search keyword '"Yun, Nayoung"' showing total 14 results
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14 results on '"Yun, Nayoung"'

2. Botanical formulation, TADIOS, alleviates lipopolysaccharide (LPS)-Induced acute lung injury in mice via modulation of the Nrf2-HO-1 signaling pathway

Author

Lee, Wonwoo, primary, Lee, Chang Hyung, additional, Lee, Jungkyu, additional, Jeong, Yoonseon, additional, Park, Jong-Hyung, additional, Nam, In-Jeong, additional, Lee, Doo Suk, additional, Lee, Hyun Myung, additional, Lee, Jaehyun, additional, Yun, Nayoung, additional, Song, Jisun, additional, Choi, Sooyeon, additional, and Kim, Sunyoung, additional

Published
2021
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4. Water-Soluble Extract from Actinidia arguta (Siebold &amp

Author

Lee, Wonwoo, Jeong, Yoonseon, Park, Jong-Hyung, Lee, Chang Hyung, Yun, Nayoung, Lee, Doo Suk, Nam, In-Jeong, Kim, Jung-Dong, Yoon, Kee Dong, Son, Miwon, and Kim, Sunyoung

Subjects
filaggrin, integumentary system, AhR, itch, ACTPER, dry-skin
Abstract

With a complex etiology involving multiple factors, the condition known as itch is a primary symptom of many skin diseases. Current treatment methods are ineffective for addressing itches caused by dry skin, for example. We developed a botanical extract, ACTPER, made from a mixture of Actinidia arguta and Perilla frutescens, which have traditionally been used to treat itch. The quality of ACTPER as a research agent was controlled in our experiment by cell-based bioassays, as well as by high-performance liquid chromatography (HPLC), using two chemical markers. In the acetone-induced dry skin mice model, the oral administration of ACTPER alleviated dry skin-related skin properties and itching behavior. The RNA and protein expression of the filament aggregating protein (filaggrin) gene, a key factor involved in the regulation of skin barrier function, was significantly increased, as measured by quantitative reverse transcription polymerase chain reaction (RT-PCR) and immunofluorescence assay. To understand the underlying mechanism(s) at the molecular level, HaCaT cells, a human keratinocyte-derived cell line, were treated with various concentrations of ACTPER. We found that the protein expression of filaggrin was indeed upregulated by ACTPER in a dose dependent manner. Data from experiments involving the reporter plasmid containing the xenobiotic response element (XRE), and the chemical antagonist for the aryl hydrocarbon receptor (AhR), indicated that the ACTPER-mediated upregulation of filaggrin was controlled through the activation of the AhR signaling pathway. The molecular docking simulation study predicted that ACTPER might contain chemical compounds that bind directly to AhR. Taken together, our results suggest that ACTPER may provide the platform, based upon which a variety of safe and effective therapeutic agents can be developed to treat itch.

Published
2019
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5. Water-Soluble Extract from Actinidia arguta (Siebold & Zucc.) Planch. ex Miq. and Perilla frutescens (L.) Britton, ACTPER, Ameliorates a Dry Skin-Induced Itch in a Mice Model and Promotes Filaggrin Expression by Activating the AhR Signaling in HaCaT Cells

Author

Lee, Wonwoo, primary, Jeong, Yoonseon, additional, Park, Jong-Hyung, additional, Lee, Chang Hyung, additional, Yun, Nayoung, additional, Lee, Doo Suk, additional, Nam, In-Jeong, additional, Kim, Jung-Dong, additional, Yoon, Kee Dong, additional, Son, Miwon, additional, and Kim, Sunyoung, additional

Published
2019
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9. Glyco-analytical multispecific proteolysis (Glyco-AMP): A simple method for detailed and quantitative glycoproteomic characterization

Author

Hua, Serenus, Hu, Chloe Y, Kim, Bum Jin, Totten, Sarah M., Oh, Myung Jin, Yun, Nayoung, Nwosu, Charles Chuks, Yoo, Jong Shin, Lebrilla, Carlito B., and An, Hyun Joo

Abstract

Despite recent advances, site-specific profiling of protein glycosylation remains a significant analytical challenge for conventional proteomic methodology. To alleviate the issue, we propose glyco-analytical multispecific proteolysis (Glyco-AMP) as a strategy for glycoproteomic characterization. Glyco-AMP consists of rapid, in-solution digestion of an analyte glycoprotein (or glycoprotein mixture) by a multispecific protease (or protease cocktail). Resulting glycopeptides are chromatographically separated by isomer-specific porous graphitized carbon nano-LC, quantified by high-resolution MS, and structurally elucidated by MS/MS. To demonstrate the consistency and customizability of Glyco-AMP methodology, the glyco-analytical performances of multispecific proteases subtilisin, pronase, and proteinase K were characterized in terms of quantitative accuracy, sensitivity, and digestion kinetics. Glyco-AMP was shown be effective on glycoprotein mixtures as well as glycoproteins with multiple glycosylation sites, providing detailed, quantitative, site- and structure-specific information about protein glycosylation.

Published
2024
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13. Abstract W P192.

Author

Jeong, Hye Seon, Kwon, Hyunjo, Koh, Hyunsong, Yu, Heesun, Yun, Nayoung, Oh, Sang-Geun, Kwag, Hyun Jeong, Song, Hee-Jung, and Kim, Jei

Published
2014

14. Botanical Formulation HX109 Ameliorates TP-Induced Benign Prostate Hyperplasia in Rat Model and Inhibits Androgen Receptor Signaling by Upregulating Ca 2+ /CaMKKβ and ATF3 in LNCaP Cells.

Author

Lim S, Lee W, Lee DS, Nam IJ, Yun N, Jeong Y, Rho T, and Kim S

Subjects
Animals, Calcium metabolism, Cell Line, Tumor, Humans, Male, Prostate drug effects, Prostate metabolism, Prostate pathology, Protective Agents pharmacology, Rats, Rats, Sprague-Dawley, Testosterone Propionate adverse effects, Activating Transcription Factor 3 metabolism, Calcium-Calmodulin-Dependent Protein Kinase Kinase metabolism, Plant Preparations pharmacology, Prostatic Hyperplasia chemically induced, Prostatic Hyperplasia metabolism, Receptors, Androgen metabolism, Signal Transduction drug effects
Abstract

Benign prostatic hyperplasia (BPH) is a common disease in the elderly male population throughout the world. Among other factors, androgen dysregulation has been known to play major roles in its pathogenesis. HX109 is a botanical formulation prepared from a mixture of Taraxacum officinale , Cuscuta australis , and Nelumbo nucifera , which have traditionally been used-usually along with other plants-to treat urinary diseases. An ethanol extract was prepared from a mixture of these three plants, and its quality was controlled through cell-based bioassays and by quantification of several marker compounds by high-performance liquid chromatography (HPLC). In the testosterone propionate (TP)-induced prostate hyperplasia rat model, oral administration of HX109 ameliorated prostate enlargement and histological changes induced by TP. In LNCaP cells, a human prostate epithelial cell line, HX109 repressed AR-mediated cell proliferation and the induction of androgen receptor (AR) target genes at the transcriptional level without affecting the translocation or expression of AR. Such effects of HX109 on AR signaling were mediated through the control of activating transcriptional factor 3 (ATF3) expression, phosphorylation of calcium/calmodulin-dependent protein kinase kinase β (CaMKKβ), and increases in intracellular calcium, as evidenced by data from experiments involving ATF3-specific siRNA, CaMKKβ inhibitor, and calcium chelator, respectively. Taken together, our data suggest that HX109 might be used as a starting point for developing therapeutic agents for the treatment of BPH.

Published
2018
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