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Circular RNA (circRNA) has been shown to participate in various tumors, including lung cancer. In the present study, we explored the expression and functional relevance of hsa_circ_0003288 in human non-small cell lung cancer (NSCLC). We verified that hsa_circ_0003288 expression was upregulated in lung cancer tissues and cell lines. Overexpression of hsa_circ_0003288 dramatically promoted lung cancer cell proliferation, colony formation, inhibited apoptosis, and increased cell migration and invasion in vitro. Xenograft experiments showed that hsa_circ_0003288 overexpression accelerated tumor growth in vivo. Mechanistically, hsa_circ_0003288 negatively regulated miR-145 to exert the oncogenic role in lung cancer. Overexpression of miR-145 decreased cell proliferation, induced apoptosis, and suppressed migration and invasion in lung cancer. Additionally, miR-145 co-transfection abolished the oncogenic role of hsa_circ_0003288. Collectively, these findings identified a novel regulatory role of hsa_circ_0003288/miR-145 axis in the progression of NSCLC.

Although clinical data suggest remarkable promise for targeting programmed cell death protein-1 (PD-1) and ligand (PD-L1) signaling in non-small-cell lung cancer (NSCLC), it is still largely undetermined which subtype of patients will be responsive to checkpoint blockade. In the present study, we explored whether PD-L1 was regulated by mutant Kirsten rat sarcoma viral oncogene homolog (KRAS), which is frequently mutated in NSCLC and results in poor prognosis and low survival rates. We verified that PD-L1 levels were dramatically increased in KRAS mutant cell lines, particularly in NCI-H441 cells with KRAS G12V mutation. Overexpression of KRAS G12V remarkably elevated PD-L1 messenger RNA and protein levels, while suppression of KRAS G12V led to decreased PD-L1 levels in NCI-H441 cells. Consistently, higher levels of PD-L1 were observed in KRAS-mutated tissues as well as tumor tissues-derived CD4+ and CD8+ T cells using a tumor xenograft in B-NDG mice. Mechanically, both in vitro and in vivo assays found that KRAS G12V upregulated PD-L1 via regulating the progression of epithelial-to-mesenchymal transition (EMT). Moreover, pembrolizumab activated the antitumor activity and decreased tumor growth with KRAS G12V mutated NSCLC. This study demonstrates that KRAS G12V mutation could induce PD-L1 expression and promote immune escape via transforming growth factor-β/EMT signaling pathway in KRAS-mutant NSCLC, providing a potential therapeutic approach for NSCLC harboring KRAS mutations.

Taking the higher vocational college students as the main study object, apply the network project management in network professional resource libraries construction and curriculum effective teaching, establish a process assessment mechanism, and then expanse students' initiative study consciousness, practical ability, innovation ability and professional extension ability.

Learning sphere and course development in higher vocational education is based on working process. This paper presents a comprehensive course development methodology of "Java Web Application Development" according to the characteristic of learning sphere and course development, as well as from the software engineering perspective. The goal is to efficiently cultivate higher technical talents.

In this thesis, we apply Power Spectrum in fabric weave recognition, use power spectrum to analyze fabric image by image lightness features at definite illumination, and then extract fabric weave texture features. We can obtain fabric weave image power spectrum curve by Fourier Transform. From the curve, we can extract direction information of fabric weave, and get fabric weft density, then calculate weft Tex, weft number, weft repeats. Experiments prove the method practical and useful for fabric weave computer automatic analysis.

In this thesis, based on the weft organization features parameters analysis results, according to warp yarn arrangement features , apply correlation coefficient and autocorrelation function in weft weave texture feature recognition, establish weft cell image, analysis the weft cell image correlation coefficient, find out the number of weft circle, set up same kind weft cell image of the same phase of weft unit, calculate the warp brightness values of the same kind weft cell image, distinguish the weft points area and warp points area through luminance signal analysis, analyze the tissue points area changes between the adjacent similar weft cell, and determine the location and density of warp yarn. Experiments prove that the Fourier-transform method is feasible and has considerable accuracy.