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Oil palm waste (OPW), comprising mainly of empty fruit bunch, mesocarp fiber, frond, trunk, and palm kernel shell generated from the palm oil industry, was collected, characterized, and then pyrolyzed to evaluate their potential to be converted into biochar. Oil Palm Empty Fruit Bunches (OPEFB) are a source of organic material with abundant nutrients and are highly potentially useful as biochar. This article provides experimental data for the production of biochar at a temperature range of 100 to 300 °C at time of 4 to 8 hours. The chemical components examined are pH, CEC, C-Organic, N-total, C/N, K dd, P, Ca, Mg, and Na, using Fourier Transform Infrared Spectroscopy (FTIR). The results showed that organic C, nitrogen, and pH were highest at 200–300 °C and had a burning time of 8 hours. Furthermore, the highest concentrations of P, Ca, and Mg were recorded at 200–300°C after 5 hours, Kdd at 100–200 °C after 5 hours, and Na and CEC at 200–300 °C after 4 hours. The transmittance intensity produced by the spectrum of hydroxyl (O-H) vibrations, carbonyl stretching (C=O), alkanes (-CH), and aromatics (C=C) decreased with increasing time, while stretching alcohol (C-O) vibrations increased with time. Our results demonstrate that OPEB is a biowaste that shows exceptional promise to be transformed into high-grade biochar rather than simply disposed of by landfilling or burning.

Neswati R, Abdullah S, Musa Y, Nasaruddin. 2023. Assessing soil conservation techniques on sloping lands in the humid tropics area of Indonesia in the context of maize cultivation. Biodiversitas 24: 1686-1692. Sloping land is prone to severe erosion, particularly in areas with high precipitation, such as in humid tropics. In addition to slope and rain factors, crop management also influences soil erosion. This research aims to identify the most appropriate techniques to mitigate soil erosion by comparing three different techniques of maize (Zea mays L.) cultivation planted on sloping land in Indonesia's humid tropics. A field experiment was conducted in Gorontalo Regency, Gorontalo Province, Indonesia, in three maize growing seasons at a location with a slope of 15-30%. We used a split-plot design in a randomized block design, with the main factor being conservation techniques, namely no treatment (control) (K0), contour terrace (K1), strip cropping using Pennisetum purpureum Schumach. (K2) and alley cropping using Gliricidia sepium (Jacq.) Kunth (K3), and the side factor was Z. mays of two varieties: Mixed variety Arjuna BC (H1) and hybrid variety NK-33 (H2). The effects of each treatment were measured in term of soil erosion, and maize growth and productivity. The results showed that the strip cropping resulted in a lower erosion level compared to the contour terrace and alley cropping. However, erosion still exceeded the tolerable soil loss in all conservation techniques. The effects on the growth and production of maize of the two varieties in growing seasons 1, 2, and 3 were not statistically different (p6 tons ha-1 in the two varieties planted and the value of the revenue to cost (R/C) ratio of all treatments ranged between 1.77 and 2.54. The results imply that the application of appropriate soil conservation methods has the potential to reduce erosion in the sloping land-humid tropics of Indonesia without compromising growth and productivity. However, it will take a longer period to reduce soil erosion significantly in a maize-based cultivation system on sloping land.

Herawati, Riadi M,MusaY, Efendi R, Azrai M. 2023. Evaluation of the growth and tolerance of maize lines under aluminum stress. Biodiversitas24: 1417-1430. Breeding and utilization of Al-tolerant maize are among the technological applications for preventing a decrease in the growth rate and grain yield of maize in suboptimal fields. This study aimed to determine the growth and production of maize lines under Al stress, obtain maize lines that are tolerant to Al stress, and determine Al absorption in the tested maize lines. This study comprised two steps. In the first step, maize lines were evaluated in acid soils with a pH of 5.12 and an Al saturation of around 22.13%, while the normal condition was carried out on soil with a pH of 6.60 and 0% Al saturation. The study was arranged in a randomized block with three replications. The maize lines used were 24 genotypes consisting of 20 maize lines and four elite lines as a check (Mal 03, CLYN 231, G102612, B11209). In the second step, maize lines were evaluated in the nutrient solution. This study was arranged in a split-plot design with three replications. The main plot was the concentration of Al at three levels, i.e., 0, 7, and 14 ppm. The subplot comprised seven maize lines representing sensitive, medium tolerant, and tolerant categories. They were Pop. A3-1, Pop. A7-1, CB.Pop 10-1-3-1-2-2, CB.Pop 15-4-2-1-1-1, CB.Pop 23-1-2-1-4-2, Mal 03, and G102612. These studies were conducted at the Indonesian Cereal Research Institute, Maros, South Sulawesi, Indonesia, from August 2021 to January 2022. This study showed that Al stress could reduce plant growth by around 10.84% to 50.51% and grain yield by around 62.23% compared to normal conditions. The traits that were significantly correlated with grain yield under Al stress (r>50%), which could be used in maize selection under Al stress, were the number of live plants, ear diameter, number of kernels per row, shelling percentage, SSI, crown Al absorption, and hematoxylin staining. There were two maize lines tolerant to Al stress, namely CB.Pop 11-2-3-4-2-1 and CB.Pop 15-4-2-1-1-1, with potential yields under Al stress conditions of 2.39 t ha-1and 1.59 t ha-1, respectively. Al absorption averages of roots and crowns of Al tolerant maize lines were 159.33 ppm and 36.33 ppm, respectively.

An unmanned aerial vehicle (UAV), widely known as a drone, proves very effective in assessing cropping or crop cultivation. Its practical use in evaluating corn cultivation technology systems is feasible when based on farmer participation. UAV can generate the Normalized Difference Vegetation Index (NDVI) algorithm that reflects the greenness of leaves, which is a parameter related to photosynthesis and plant productivity. Therefore, the purpose of this study was to evaluate whether the participation-based UAV-derived NDVI could be effectively used to assess corn cultivation technology and determine the appropriate technology to be used in the cultivation. The research was conducted in Tarowang Village in Galesong Selatan District, Takalar Regency, South Sulawesi, Indonesia, using two plots, namely, mother trial and baby trial. The mother trial applied a randomized block design in which eight packages of corn cultivation technology were randomly assigned, whereas the baby trial consisted of eight corn plots cultivated by farmers. In the latter, each farmer received one package of the cultivation technology. The study results indicated that NDVI and yield could effectively evaluate corn cropping. Three packages, i.e., P1, P4, and P5, are recommended for corn cultivation, especially in the village observed. Nevertheless, they are expected to be also applicable to other districts in South Sulawesi to promote improvement in corn production.

Kamsurya MY, Ala A, Musa Y, Rafiuddin. 2022. Short Communication: Correlation of flowering phenology and heat unit of forest cloves (Syzygium obtusifolium) at different elevations in Maluku Province, Indonesia. Biodiversitas 23: 5593-5599. The phenology and heat unit of the forest clove (Syzygium obtusifolium L.) are important parameters for the efficient maintenance of this plant. This study aimed to describe phenological flowering stages (PFS) and calculate growing degree days for different PFS, which will have a big impact on crop management and understanding the impact of climate variables on crucial phenological events. The research was conducted in Ambon, Maluku Province, Indonesia, for 16 months, from January 2020 to May 2021. Five sample points at different elevations were determined purposively, i.e: Halasi (6 m above sea level (m asl)), Tibang (11 m asl), Oli (125 m asl), Wanat (175 m asl), and Telaga Kodok (214 m asl). The phenology of flowering is separated into 6 stages: the flowering primordia, the appearance of the flower stalk, the perfect flower stalk, the appearance of the flower bud, the flower is fully formed, and the flower starts to bloom. The results showed that the phenological cycle of the forest clove plant was flowering periodically for 365 days. The average temperature of PFS (29oC) in Halasi was higher compared to other locations. It indicates that the lower the altitude, the faster the flowering period. This study found significant correlations between altitude and PFS, except flower stalks appear and flowers will appear. The phenology of clove plants is influenced by the accumulation of heat units, with the highest heat unit value in the flowers start to bloom stage, ranging from 2,983.23 dayso (Telaga Kodok) to 3,128.84 dayso (Halasi). As elevation increases, the number of heat units decreases, so the PFS is delayed. The primordial phase in Halasi appears in August-September while at Telaga Kodok in December.

This study examines the introduction of a combination of cocoa husk biochar which has been a cocoa plantation waste combined with fermented cocoa leaf waste. This study was conducted in West Sulawesi. This study used a randomized block design using a factorial model where the first factor was the administration of cocoa husk biochar with 3 levels, namely without administration (B0), 6 kg plant biochar-1 (B1), 12 kg plant biochar-1(B2) while the second factor was application of fermented cocoa leaf waste (K) which consists of 3 levels, namely without giving cocoa leaf waste (K0), giving 6 kg plant-1 (K1), biochar 12 kg plant-1 (K2). The nine treatment combinations were repeated three times to obtain 27 plant samples. The analysed soil characteristics are calcium (Ca), magnesium (Mg) and potassium (K) showed that the combination of cocoa rind Biochar and fermented cocoa leaf waste had an effect on increasing calcium (Ca) by 9.23. Magnesium 1.66 and for the element Potassium had no significant effect on the interaction of the combination of cocoa husk biochar and fermented cocoa leaf waste, but gave a very significant effect on the administration of biochar 12 kg plants-1.

The exploration of arbuscular mycorrhiza fungi from sugarcane plantation in marginal land in South Sulawesi was carried out to find the source of inoculums showing effective infection. Soil samples were taken from four area with different characteristic of marginal land, namely land with low organic matter content, clay texture, limited irrigation, and undulating land. Mycorrhizae contained in the soil samples were then observed, and the spores obtained were used as the source of isolation by a single spore culture. The mycorrhizal spores were isolated by wet sieving and centrifugation method with 48% sucrose, which were observed under a compound microscope for spore details (100-1000x). Sugarcane root samples were taken to observe mycorrhizal infection in sugarcane root tissue by root staining method. The results of the study showed that the greatest diversity of mycorrhizal genera was found in soil samples of Jambua Block (Glomus, Gigaspora, and Sclerocistis) and AJ-5 Block area (Glomus, Acaulospora, and Sclerocistis). Single-spore isolates obtained were Glomus sp. and Acaulospora sp. Infection test result on four sugarcane varieties commonly grown in Takalar Sugar Factory showed that infectivity of mycorrhizal isolate of Acaulospora sp. was the highest (75%) and significantly different (LSD’test, p 0,05) compared to that of Glomus sp. (66%).

The low productivity of cocoa plantations in Indonesia is partly due to the low quality of seeds, which refers to the impeded growth of cultivated cocoa nurseries. Seed is the initial growth of plants so the importance of giving special treatment to seeds will refer to better seed growth. Provision of Plant Growth Promoting Rhizobacteria (PGPR) microbes can produce indoleacetic acid (IAA) in plants to improve the quality of plant growth. This study aims to determine the effectiveness of the provision of Plant Growth Promoting Rhizobacteria bamboo rhizosphere against cocoa seed germination. The study was carried out in the farmer group garden, Gantarangkeke District, Bantaeng. This study was arranged in the form of a two-factor factorial design (F2F) in a randomized block design (RBD). The use of cocoa seed type as the first factor consisted of GTB (Gantarangkeke Bantaeng) local cocoa seed and MCC 01 cocoa seed and seed immersion treatment at PGPR rhizosphere bamboo concentration as the second factor consisting of 0% (control) concentration, 5%, 10 % and 15%. The results obtained indicate that administration of seeds with bamboo rhizosphere PGPR affects the germination (100.00%), the speed of seed growth (7.14%/etmal), as well as on abnormal seeds (10.00%). So that the provision of bamboo rhizosphere PGPR on cocoa seeds has an effective influence on seed germination and cocoa seedling development.

Penelitian ini bertujuan untuk mengetahui genotipe mutan jagung yang berkerabat paling jauh dengan induknya serta mendapatkan genotipe jagung yang adaptif dan toleran terhadap kekeringan. Penelitian ini dilaksanakan pada bulan Juli - November 2014 di Laboratorium Biologi Molekuler Balitsereal Maros dan lahan di Desa Bontosunggu Kecamatan Galesong Utara Kabupaten Takalar. Penelitian ini menggunakan metode isolasi DNA marka SSRs (Simple Sequence Repeats) terhadap 14 genotipe jagung dan uji adaptasi di lahan kering menggunakan Rancangan Petak Terpisah dalam 3 ulangan dengan petak utama perlakuan pengairan yang terdiri atas tanpa pengairan dengan pengairan normal. Anak petak yaitu perlakuan genotipe dengan tingkat ketersediaan air (KA) yang berbeda-beda pada penelitian sebelumnya diantaranya Bisma 100 Gy KA 60%, Bisma 100 Gy KA 80%, Bisma 100 Gy KA 100%, Bisma 200 Gy KA 60%, Bisma 200 Gy KA 80%), Bisma 200 Gy KA 100%, Lamuru 100 Gy KA 60%, Lamuru 100 Gy KA 80%, Lamuru 100 Gy KA 100%, Lamuru 200 Gy KA 60%, Lamuru 200 Gy KA 80%, Lamuru 200 Gy KA 100%, Bisma tanpa iradiasi, dan Lamuru tanpa iradiasi. Hasil penelitian di laboratorium menunjukkan bahwa 34 – 91 % polimorfisme dalam suatu populasi dimana genotipe Bisma 100 Gy dan Bisma 200 Gy yang sebelumnya ditanam pada kondisi KA masing-masing 100%, genotipe Lamuru 100 Gy dan Lamuru 200 Gy yang sebelumnya ditanam pada KA masing-masing 60%, serta genotipe Lamuru 200 Gy yang sebelumnya ditanam pada KA 80% mempunyai kekerabatan paling jauh dengan induknya. Uji adaptasi di lapangan memperlihatkan hasil bahwa genotipe berasal dari Bisma 200 Gy yang dianggap toleran terhadap kekeringan dengan nilai tertinggi pada berbagai komponen parameter pengamatan di lapangan. Parameter pengamatan tinggi tanaman, jumlah daun, bobot tongkol kupasan basah, jumlah biji per baris, dan diameter tongkol merupakan karakter yang dapat dipakai sebagai tolok ukur ketahanan terhadap cekaman kekeringan

This study aims to analyze the productivity and income of farmers on the pattern row planting legowo and tiles as well as formulate alternative strategies that are appropriate for the development of paddy rice rain-fed in an effort to increase productivity and income of farmers in the District Moncongloe Maros Regency of South Sulawesi. The research method used is purposive sampling based on the type of rice varieties used, namely Inpari 33 with the pattern of row planting legowo and tiles as well as rainfed land. The sample taken is a member of the farmer group Biring Jene 1 and farmer group Biring Jene 2. Two of these groups are selected because members of this group there that uses the pattern legowo row planting and using varieties Inpari 33. The results of the research that the amount of revenue jajar legowo higher compared to tiles with at 9.358,3 kg/Ha or Rp. 42.112.350 to forecast legowo row planting and 4.185,4 kg/Ha or Rp. 18.834.449 for the reception of grain production in the cropping pattern tiles, with a difference of the receipt of such production of 5.172,9 kg/Ha or Rp. 23.277.901 and the value of the R/C ratio of 1,53 jajar legowo and of 1.49 for the tiles. The formulation of the strategy of SWOT analysis is a Strategy S-O, among others, the Development and improve the human resource capabilities of farmers in agricultural technology innovation, strategy W-O, among others, improve the ability mastery of appropriate technology for farmers, the strategy S-T, among others, the optimization of the agricultural land and the strategy of W-T, among others, use varieties that are resistant to PEST and disease and able to survive extreme conditions.

The research was aimed to obtain male and female rows ratio and best pruning technique towards SINHAS 1 maize production. This research was conducted in Experimental Farm Faculty of Agriculture, Hasanuddin University, Tamalanrea District, Makassar, South Sulawesi with elevation of 22,4 meters above the sea level throughout May – August 2020. The experiment was carried out in the form of Split Plot Design with male-female row ratio as main plot, which consisted of three levels: 1:2 ratio, 1:3 ratio, and 1:4 ratio. Sub plot was pruning techniques: no pruning, male parent pruning and leaf pruning under the cob of female parent. The result showed that the favorable male-female row ratio towards SINHAS 1 maize production was 1:4 ratio. Male parent pruning was the best pruning technique for SINHAS 1 maize production. The interaction between male-female ratio and best pruning technique was 1:3 male-female ratio with male parent pruning technique. Characters that showed positively direct significance towards productivity were cob diameter, cob length, and number of seed rows per cob

One of approaches to maintain the yield stability of the lowland tropical wheat is the use of secondary traits in the selection process. The identification of these characters requires a statistical approach in the form of genetic parameter analysis and multivariate analysis. The objective of this study was to determine the secondary traits of adaptive wheat mutants in the lowlands through the use of genetic parameters and multivariate analysis on the parameters. The study consisted of three field trials conducted in three different regencies, namely Jeneponto (135 m above sea level (asl)), Maros (100 m asl) and Bantaeng (125 m asl). The study used a nested design, where replications were nested in the environments. The genotype factors consisted of 20 genotypes repeated three times. 11 characters were observed including vegetative and reproductive characters. The analysis used consisted of repeatability, correlation, cluster analysis, principal component analysis, factor analysis, and cross print analysis. The overall results of the analysis indicate that the number of productive tillers is the main secondary trait for the selection of adaptive wheat in the lowlands. The character can be recommended for selection criteria in testing wheat lines in the lowlands to make an effective selection.

Salinity screening under hydroponic Deep Flow Technique (DFT) has not been widely studied, especially on the nature of rice tolerance to salinity stress. According to previous screening studies, this method was effective in distinguishing the nature of rice tolerance to salinity stress. However, they were tested only at generative phase. Therefore, evaluation on screening method with hydroponic DFT at all phases of paddy growth is essential. The objective of this study is to evaluate the filtering under hydroponic DFT at all paddy phase and to determine secondary character that support productivity which can be utilized as selection character in this screening process. The experiment was arranged with a complete nested group design with nested replication is the NaCl stress. There were 5 (Five) tested rice varieties and the stress environment consisted of three levels: 0, 60, and 120 mM of NaCl, all with 3 (three) replications. The nutrient culture screening was adapted to the modified Egdane method. The results showed that screening under hydroponic DFT was effective at the concentration of 60 nM of NaCl. The best selection character was yields per clump, number of productive tillers and total chlorophyll. The variety of Jeliteng, Ciherang and Inpari 34 of Salin Agritan, were classified as tolerant group. This hydroponic DFT Filtering method could be recommended as one salinity screening method for all paddy growing phases

Padjung R, Farid M, Musa Y, Anshori MF, Nur A, Masnenong A. 2021. Drought-adapted maize line based on morphophysiological selection index. Biodiversitas 22: 4028-4035. Synthetic line formation is an effort to increase maize productivity in drought-stressed areas. This process requires systematic selection in determining adaptability levels involving important secondary characters formulated in the selection index. Furthermore, the principal component index had been widely reported, both in normal and stressed conditions. The selection index development on morpho-physiological characters based on multivariate analysis was expected to increase drought stress tolerance and maize lines selection precision. Therefore, the purpose of this research was to form a selection index based on morpho-physiological characters and selecting synthetic lines adaptive under drought stress. This research was designed using a split-plot with 3 replications, where the main plot consisted of normal and stressed irrigation, while the subplot was genotype consisting of 6 lines and 3 check varieties namely Bisma, Lamuru, and Sukamarga. Observations were performed on 18 morphological and 4 physiological characters, and the results showed that the selection index was formed based on multivariate analysis from 9 characters. Through index selection, Syn_2-2 (0.79), Syn_2-15 (0.85) and Syn_2-16 (0.97) were considered as drought stress adaptive lines. Therefore, the 3 lines can be recommended in the synthetic variety release process.

Salinity stress can reduce rice (Oryza sativa L.) productivity and cause crop failure. This problem needs a solution by development of tolerant varieties, and this development closely relates to the effectiveness of its screening. Screening at the phase of germination and seedling are the most common ones. However, the interactions between screenings based on the growth character selection index have not been widely reported, particularly with the principal component analysis (PCA). Therefore, the aim of this study was to determine the interaction between phases of rice salinity screening through a selection index based on PCA. This study consisted of two phases of salinity screening, namely growth phase of germination and seedlings. Both screenings were designed with a nested randomized complete block design, where replicates were nested in a selection environment. The selection environment consisted of two levels, normal (0 mM NaCl) and saline (120 mM NaCl). The genotypes consisted of eight varieties and was repeated three times. Observations were based on morphological and physiological characters, especially in seedling phase screening. Results showed that morphological character approach of the seedlings had a large distribution of salinity tolerances. The use of stress tolerance index and PCA were considered effective in the formation of the selection index on salinity screening. As for, the morphology index was formulated as 0.32 shoot height + 0.33 root fresh weight + 0.33 shoot fresh weight + 0.26 root length + 0.01 number of tillers, length + 0.34 total biomass fresh weight. Therefore, the use of this analytical concept is recommended in screening the tolerance of rice lines to salinity stress.

Wheat is a sub-tropical plant that can adapt well at altitudes of 1000 - 3000 m above sea level and requires relatively low temperatures. At this height, wheat crops in Indonesia are unable to compete with horticultural crops with higher economic value. This causes national wheat production to be very low and results in high wheat imports. Therefore, wheat varieties are needed that can grow and develop in Indonesia in the low to medium plains. The study aimed to test the adaptive mutant population descent in the middle plains to prepare multi-location tests and release of varieties. The benefit of this research is obtaining potential strains from high-temperature adaptive wheat mutants in the lowlands. This research was conducted using a randomized block design with three replications. The treatment consisted of 16 M6 Wheat mutants and four comparative varieties. The results showed that the genotypes of wheat mutants that had high production in M6 propagation in the central plains were N 350 3.7.1 (2.74 t. ha-1), N 350 3.6.2 (2.33 t.ha-1) and N 350 3.1.3 (2.26 t.ha-1). Characters that have high heritability values on M6 Number of stomata, chlorophyll index, plant height, number of tillers, productive tillers, rate of seed filling, panicle length, number of seedlings, empty percentage of florets, hollow seed weight, 1000 seed weight, and production

Sugarcane requires land conditions that contain high organic matter to support growth and production. Giving compost from the results of the utilization of the byproducts of sugarcane processing in the form of bagasse and filter mud is expected to increase the sugarcane productivity. The purpose of this study was to determine the effect of compost from the sugarcane bagasse, filter mud and cow manure using the decomposer and Trico Plus mushroom consortium on the growth and production of sugarcane. The experiment was carried out in the form of a randomized block design consisting of eight treatments and three replications. Each treatment was applied as much as 15 tons per hectare, NPK basic fertilizer for all 350 kg treatments per hectare. The results showed that the treatment of compost provides a higher number of sugarcane saplings compared to without the provision of compost. The compost C treatment gave the highest number of sugarcane saplings and the highest number of segments of 7.45 sugarcane saplings and 18.33 segments. Treatment G gave the highest plant height of 283.30 cm, treatment B gave the highest segment length of 15.17 cm, stem diameter of 2.62 cm, stem height of 163.00 cm, sugarcane stem weight of 158.36 kg per plot and 97.655 tons per hectare and POL value 31.32% while treatment E gave the highest Briks value of 23.43%. G treatment gave the best plant height value of 283.30 cm. Giving compost from the results of mixing bagasse, filter mud and manure using the mushroom and Trico Plus consortiums gave better results on growth, and production of sugar cane compared to without the provision of compost.

This research was aimed to evaluate the efectivity of screening method and identification of tolerance screening selection character in statis hydroponic system towards drough stress. The research was carried out in Hydroponic Screenhouse Perdos Unhas, Tamalanrea, Makassar which throughout February-April 2020. This research was conducted in factorial design in nested patterns, where environment was the nested replication. Environments used were normal (0% PEG) and drought stress (10% PEG). Five rice varieties were used: Inpari 34, Ciherang, IR29 and Jeliteng. Research result showed that canopy height and fresh weight were the suitable selection character in drought tolerance screening through statis hydroponic cultivation. Grouping consistency was found between drought and salinity stress. Inpari 34, Ciherang, Jeliteng and Inpari 29 was considered to be have drought tolerance, whilst IR29 was examined to be responsive to drough stress in hydroponic culture. From the overall result, it can be concluded that hydroponic screening and PEG application was quite effective in rice drought stress tolerance screening

A study and identificiation on agrobiodiversity of refugia plant as alternative solution by farmers towards their several plant gardens to repel pest was conducted at Tulung Rejo, East Java, Indonesia in March 2021. Refugia plants are biodiversity plants that grow around cultivated plants, which have the potential as a place of protection and a source of food for natural enemy insects (both predators and parasitoids). The principle of refugia pants is that these plants can attract and become a place of life and a source of life for insects, natural enemies of pests. the reseach was using a survey method with interview with farmers who have a land and using refugia plant as a natural pesticides to repel pests of their plants. besides, this research was also using a physical indentification of several refugia plants. Among several locations in Tulung Rejo, the results show that on average farmers use refugia plants of the type of kenikir flower Cosmos caudatus. This has evident because from 20 garden locations, 18 of them use Cosmos caudatus. The needs of farmers in Tulung Rejo, East Java to repel pests naturally.

This study aims to investigate the growth and productivity of Arabica coffee plants due to different plant maintenance. The research was conducted from November to December 2019 in Lembang Kayuosing, Rembon District, Tana Toraja Regency, South Sulawesi Province. The study used survey and observation methods with questionnaire/interview techniques. Data was analysed based on multiple linear regression using SPSS software. The analysis was used to determine the effect of plant maintenance and different types of shading trees. The maintenance techniques that significantly affect the productivity of Arabica coffee plants in Lembang Kayuosing, Rembon District, Tana Toraja Regency were fertilization methods, fertilization time, shape pruning, and pests and plant diseases control. As for the best type of shade tree that significantly affected coffee plant height and stem diameter was Sengon (Albizia chinensis) plant. The productivity of Arabica coffee plants in Lembang Kayuosing, Rembon District, Tana Toraja Regency was 0.47 kg/tree.

The research aims were to analyse the effect of bacterial mixture of Azotobacter and Azospirrilum on the nitrogen content and harvest date of upland red rice. This research was conducted in Agriculture Farm of Agriculture Faculty in Halu Oleo University. The research was arranged in a split plot design. The bacterial mixture dosage as main plot and different of cultivar as sub plot. The bacterial mixture dosage were without application bacterial mixture, Bacterial combination mixture of Azotobacter 2.5 L ha−1 + Azospirillum 2.5 L ha−1, and Bacterial combination mixture of Azotobacter 5.0 L ha−1 + Azospirillum 5.0 L ha−1. The cultivar tested were Labandiri, Jangkobembe, Ranggohitam, and Paedara. This research shows that application of bacterial mixture had no significant effect on nitrogen leaf content, but had a significant effect on flowering time and harvesting. It was found that flowering time tended to accelerate, but on harvesting-date parameters tended to be prolonged.

The development of drought rice screening is one of the keys to increase selection effectiveness. This development can be done by developing the analytical method. In general, identification of tolerant rice can be conducted with the cluster analysis. However, the common cluster analysis just was focused on genotype clusters so that the reason for the clustering does not can explain. Therefore, the other analysis approach needs to be done, such as cluster heatmap analysis. The objective of this study is to identify the effectiveness of cluster heatmap used in rice tolerance screening under drought stress. This study was designed with a nested randomized complete group design, where replications were nested in PEG 6000 concentration as a screening environment. The concentration of PEG used in this study was 0% PEG and 20% PEG. The genotype used consisted of 8 genotypes repeated three times. Hydroponic culture used ABmix in culture solution. As for, the number of characters observed was seven morphology characters and three physiological characters. The results of this study showed that cluster heatmap analysis could distinguish between the rice tolerant group drought-tolerant variety control (Salumpikit, Pokkali, and Inpari 29), and sensitive variety control (IR 20). Besides that, the good selection characters in hydroponic drought screening were shoot length, the number of tillers, shoot fresh weight, root fresh weight, and total biomass fresh weight. Based on this study, the cluster heatmap can be recommended as one of the analytical methods in hydroponic drought screening.

The aim of this study was to determine the growth of sugarcane seeds using Trichoderma application and different parts of the stems using the single bud method. The research was conducted at the Experimental Garden at the Faculty of Agriculture, Hasanuddin University, Tamalanrea, Makassar. The research took place from July to November 2018. This research was conducted in the form of a two-factor factorial experiment which was arranged based on a randomized block design. The first factor is Trichoderma with 3 levels of doses, namely without Trichoderma, 15 g/polybag and 30 g/polybag, while the second factor is the stem which consists of the upper stem, middle stem and lower stem which consists of 9 treatment combinations, respectively. -Each treatment was repeated 3 times (3 polybags/treatment) so that the total poly bag used was 81. The results showed that treatment without Trichoderma gave the best results on plant height, namely 13.91 cm at 2 WAP. While the treatment of the middle stem gave the best results on the number of tillers, namely 10.71.

Development of adaptability rice under salinity stress needs effective and selective methods in the screening process. The seedling screening method is a general method used in salinity screening. However, this screening method often uses conventional observation in its screening process. This observation is rated that has a high error level. Therefore, the development of a digital approach through image-based phenotyping expected could minimize the error in the adaptability screening. This study was designed with a nested randomized complete group design, where replications were nested in a stressful environment. The environment in this study was normal (0 mM NaCl) and salinity stress (120 mM NaCl). The genotype used consisted of 8 genotypes which were repeated three times. The number of characters observed was nine image-based phenotyping. The results of this study showed that green percentage, the 3rd leaf length, and total area were the selection characters of image-based phenotyping under seedling salinity screening. Besides that, the used adaptability index in salinity screening became a good approach in considered and distinguished tolerance responses among varieties, especially to Pokkali (tolerant control variety) and IR 29 (sensitive control variety). Based on this study, the application of image-based phenotyping recommended in the screening process of line adaptability under salinity stress.

High temperature stresses is a challenge for developing the wheat in tropical environments and affect each stage of plant development. Therefore, early detection of yield characteristics using high-temperature linked markers is necessary in order to produce adaptive varieties in tropical environments. The purpose of this study was to detect differences in the formation of band patterns from tropical wheat populations from field selection using SSR markers for heat tolerant. This experiment used 10 selected genotypes and tested with 10 SSR markers for heat tolerant. The results showed that from 10 SSR primers only 2 primers showed a difference in band size, namely on the XGWM285 and XBARC197 primers which were formed only in OHP12a1-1-9 genotypes.Keywords : Tropical wheat, Simple sequence repeat (SSR) marker, heat tolerant, Genotypes

The use of True Shallot Seeds (TSS) as an alternative planting material to produce quality shallots (Allium cepa L.), is constrained by the low formed of flowers and seeds. This study investigates the effect of potassium nitrate (KNO3) concentration on the flower and seeds formation of three shallots cultivars, and was conducted from September 2019 to January 2020 in District of West Sinjai, Sinjai Regency, South Sulawesi, at 1250 m above sea level. The experiments was set up as split plots design and provided with 3 replications. The main plot was shallots varieties (Sanren, Superpilip, and Tajuk) and the subplot was PN dosage (0, 7, 9, and 11 g per plant). Experimental results revealed that interaction of PN and shallots varieties gave a significant effect onthe average number of flower buds, number of filled flower capsules, and seeds weight. Application of 11 g PN on Superpilip varieties was able to produce the highest average number of flower buds (63), number of filled flower capsules (66.62), and seed weight (4.4 grams). This finding concludes that application of 11 g potassium nitrate per plantinduce flowering and increase seeds production of shallots, however the results will vary depend on the varieties.

This study aimed to determine the effect of mycorrhizal utilization on the growth of sugar cane seedlings with bud chips system. The research was conducted in the form of a two-factor factorial experiment arranged based on a Randomized Group Design with 3 replications. The first factor was varieties with three levels namely Bulu Lawang, Kidang Kencana, and Camming while the second factor was mycorrhizae: Glomus sp 1, Acaulupora sp, and Glomus sp 2. The results showed that Kidang Kencana variety gave the highest average values in plant height and brix levels. Whereas Camming variety had the highest average value in stem diameter, root weight and root length. Glomus sp 1 mycorrhiza was a type that associated well in sugarcane compared to Acauluspora sp and Glomus sp 2. The combination of Camming variety and Glomus sp 2 mycorrhiza produced the highest average number of leaves at 2 weeks after planting (WAP).

An experiment was conducted to determine and study the effect of adding Trichoderma sp. on compost for the growth of Arabica coffee seedlings (Coffea arabica L.). The research design was a complete randomized design (CRD) of a two-factor factorial pattern. Compost was the first factor consisted of 4 levels: 0 g per polybag, 100 g per polybag, 200 g per polybag, 300 g per polybag, and Trichoderma sp addition as the second factor consisting of 4 levels, namely: 0 g per polybag, 1 g per polybag, 2 g per polybag, 3 g per polybag. The results showed that the compost dosage of 300 g / polybag gave the best results on average leaf area parameters (22.22 cm), stem diameter (3.27 mm), and the highest percentage of succesful shoot grafting which was 86.11%. Addition of 2 g Trichoderma sp. per polybag gave the best results on leaf area at the age of 6 week after planting of 6.38 cm.

This study aimed to identify and formulate the influence and correlation of farmers’ practices and understanding related to the shading and pruning on the yiels of their Arabica coffee. The research was carried out in Bolokan Village, Lembang Tiroan Bittuang, Tana Toraja Regency, South Sulawesi-Indonesia. The research was in the form of surveys (study literature, observations, and interviews), with the selection of samples carried out deliberately (purposive sampling). The data was analysed with multiple linear regression using SPSS software. The results showed that the different levels of practices related with shading and pruning management provided significant effect and correlation with the yields of coffee plants. The generated multiple linear regression equation was Y = 27.983 + 1.131X1 + 0.591X2 + 2.575X3 + 1.050X4 + 0.820X5 + 1.967X6 + 1.185X7 + 0.313X8 + 0.823X9. Factors from aspects of maintenance techniques related with shading and pruning could increase the production and productivity of Arabica coffee plants. Variables of plant height adjustment, flowering and fruiting stimulation through shading, production pruning, understanding of shade plants benefits and pests and diseases control were stated to have an influence on yields (fruit) of coffee plants, while shade management, pruning time, rejuvenation pruning, and permanent shade plants were declared to have no significant effect.

A study aimed to evaluate the growth and production of several synthetic maize lines at low nitrogen level was conducted at the Indonesian Cereals Research Institute Experimental Farm in Bajeng District, Gowa Regency, South Sulawesi. The research was carried out from September to December 2018. The study also aimed to determine the nitrogen dose that could be used for selection of synthetic maize genotypes against low nitrogen doses. The study was conducted in the form of an experiment using a Split Plot Design. Five levels of Nitrogen fertilizer were used namely 0, 50, 100, 150, and 200 kg N per hectare. Various genotypes treatment were consisted of 4 synthetic maize genotypes namely Syn 5, Syn 6, Syn 7, Syn 8 and 2 comparative varieties namely Bisma and Lamuru. The results show a significant interaction between the treatment of nitrogen doses and the synthetic maize genotype on the morphological and physiological characters of the maize genotypes. Genotypes gave the best results at nitrogen doses of 100 kg N ha−1 was Syn8 (8.67 tons ha−1) and 50 kg N ha−1 (6.47 tons ha−1). Nitrogen doses of 50 kg N ha−1 and 100 kg N ha−1 can be used for the selection of maize tolerant to low nitrogen condition with productivity of 4.57 tons ha−1 and 8.67 tons ha−1, respectively. There is potential for the Syn8 genotype to be developed for low-nitrogen-tolerant maize varieties due to higher production compared to the comparative variety.

The aims of this research were to analyze the effect of dual inoculation of Azotobacter and Azospirrilum on the productive trait upland red rice cultivar. The research was conducted in the field of experimental farm, department of agronomy, the faculty of agriculture, Halu Oleo University. This research arranged in split-plot design with dual inoculation treatment in the main plot and cultivar differences in the subplot, with three replications. The dual inoculation treatment consist of three levels as follows: b0 = without inoculation, b1 = treatment with Azotobacter 2.5 L ha−1 + Azospirillum 2.5 L ha−1 and b2 = Treatment with Azotobacter 5.0 L ha−1 + Azospirillum 5.0 L ha−1. The type of cultivar used were (v1) = Labandiri, (v2) = Jangkobembe, (v3) = Ranggohitam and (v4) = Paedara. The result showed that the bacterial fixator application would (1) increase the productive tillers, and (2) increase rice productivity through the increase of productive tillers and grain weight.

Plant Growth Promoting Rhizobacteria is a group of microorganism that is able to increase plant growth and yield through reactions in the soil, but the large number of microorganisms contained in PGPR's rhizosphere from thorn bamboo make it difficult to know which bacteria are the most dominant and most active in influencing plants. This study aimed to determine the effect of using the 3% gram KOH test method in identifying PGPR bacteria from the thorn bamboo rhizosphere. This test was carried out at the Biofertilizer and Potential Microbial Laboratory, Faculty of Agriculture, Universitas Hasanuddin from October-December 2019. The testing methods include making microorganism growing media, pouring media, planting bacteria with the scatter and scratch methods, also the gram reaction test using KOH 3%. The results obtained the use of the 3% gram KOH test method in the scatter method showed there were 2 samples that have mucus with a percentage of 40%, while in the scratch method showed all samples have mucus with a percentage of 100% so that the use of the 3% gram KOH test method has an optimum effect in detecting microorganisms especially in identifying plant growth promoting rhizobacteria.

Palmae is a tree family that has the third biggest potential after the Gramineae and Fabaceae families. Arenga belongs to the genus of the multipurpose palm family (Mogea, Seibert, and Smits 1991), famously known as the “tree of life” and it has high economic potential, especially its sap. The sap produced is the result of photosynthesis which has a high amount of sugar with various inorganic substances and nutrients and good for health. This research provided information regarding the association of sugar content with morphological characteristics and environmental factors, in this case, the intensity of light, humidity and elevation. Morphological parameters observed were stem height, the girth of the bunch, number of green leaves, length of petiole, leaf width (lad), leaf length (pad), number of the female and male inflorescence. Correlation analysis between morphological characters and Brix content showed a moderate correlation on length of the leaf, number of green leaves, and stem height. While the character of environmental factors to Brix content had a moderate correlation on light intensity below the tree stand with a negative correlation value, which was not in line with the value of confidence interval (UpperCI = 0.004) and significantly affected the Brix content.

Wheat as a cereal crop has a strategic role in supporting food security and to meet human needs on food. Nutritional content of wheat is relatively higher than other cereal plants. This research aimed to study the adaptability, growth, and production of some wheat mutant lines at various water availability in the lowlands. The research was carried out in the Experimental Garden, Faculty of Agriculture, Universitas Hasanuddin from November 2017 to January 2018. Location of the trial was at an altitude of < 40 meters above sea level with an average temperature of 24 ºC in the morning and 32 ºC in the afternoon. A split plot design was employed in this study with the availability of water set as the main plot and the wheat mutant genotypes as subplots. Three levels of water availability was used, 81-100% field capacity, 61-80% field capacity, and 41-60% field capacity. The wheat mutant genotypes used consisted of 15 genotypes (13 mutants and 2 comparative varieties). The results of the research show that wheat genotypes grown in the lowland that showed better production in restricted water availability (61 - 80% of field capacity) were Nias 250 4.6.2 (0.95 g. plant−1), and Nias 350 3.8. 9 (1.10 g.plant−1). Whereas in the 41 - 60% of the field capacity, the Nias 250 4.6.2 and Nias 350 3.8.9 produced better yield of 0.40 g.plant−1.

Maize is one of the strategic commodities in economic development in Indonesia. The need for maize in Indonesia continues to increase. Adequacy of nutrients affects the physiological process, where Boron (B) is one of the essential nutrients for optimal plant growth, development, yield, and quality. Nutrition B is important in the development of reproductive organs, components of corn and grain yield. This study aims to examine the effect of B on growth and flowering and its effect on yield components and grain yields of prolific maize hybrid. The study was conducted using Separate Plot Design, as the main plot was Bisi 2 variety and Nasa 29 while subplot was level B: 0 kg B ha-1, 0.25 kg B ha-1, and 0.50 kg B ha-1, by foliar application. The results showed that There is no significant difference between Bisi 2 Variety and NASA 29 in flowering parameters, yield components and yield of seeds except for the number of tassel branches and 1000 grains weight. application B up to 0.50 kg B ha−1 showed the highest results and significantly increased the number of tassel branches 5.10%, tassel length 8.62%, and silk dry weight of 29.54%, cob length 6.89%, grain weight per plant 15.69%, 1000 grains weight 8.36% and grain yield (moisture content 15%) 15.80% of 0 kg B ha−1. Application B 0.50 kg B ha−1 can be recommended in the Bisi 2 and Nasa 29 especially in low soils B.