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The present study addressed whether watching entertainment videos in succession could have a positive effect on the mental health of undergraduate students. Two experiments were designed. One hundred and sixteen university students participated in experiment 1. It aimed to explore whether watching motivational videos pushed by WeChat in continuous four weeks could affect mental health at the individual level, including mental health level and achievement goal orientation level. Experiment 2 enrolled 108 undergraduate students. It aimed to explore whether watching motivational and comedy videos pushed by WeChat in continuous four weeks could affect the mental health of undergraduate students at the social adaptation level, including interpersonal relationships and class atmosphere level. Results showed that entertainment videos pushed by WeChat in succession have significant positive effects on the university students' mental health and positive psychological quality.

Fruit postharvest ripening is a crucial course for many fruits with significant conversion of biosubstance, which forms an intricate regulatory network. Ethylene facilitates the ripening process in banana with a remarkable change of fruit starch, but the mechanism adjusting the expression of starch degradation-related enzyme genes is incompletely discovered. Here, we describe a banana APETALA2 transcription factor (MaAP2a) identified as a transcriptional repressor with its powerful transcriptional inhibitory activity. The transcriptional level of MaAP2a gradually decreased with the transition of banana fruit ripening, suggesting a passive role of MaAP2a in banana fruit ripening. Moreover, MaAP2a is a classic nucleoprotein and encompasses transcriptional repressor domain (EAR, LxLxLx). More specifically, protein–DNA interaction assays found that MaAP2a repressed the expression of 15 starch degradation-related genes comprising MaGWD1, MaPWD1, MaSEX4, MaLSF1, MaBAM1-MaBAM3, MaAMY2B/2C/3A/3C, MaMEX1/2, and MapGlcT2-1/2-2 via binding to the GCC-box or AT-rich motif of their promoters. Overall, these results reveal an original MaAP2a-mediated negative regulatory network involved in banana postharvest starch breakdown, which advances our cognition on banana fruit ripening and offers additional reference values for banana varietal improvement.

Nitrogen (N) is an essential element in the biosynthesis of key cellular components, such as proteins and nucleic acids, in all living organisms. Nitrite, as a form of nitrogen utilization, is the main nutrient for microbial growth. However, nitrite is a potential carcinogen that combines with secondary amines, which are breakdown products of proteins, to produce N-nitroso compounds that are strongly carcinogenic. Nitrite reductase (Nir) produced by microorganisms can reduce nitrite. Binding of GlnR to the promoter of nitrogen metabolism gene can regulate the expression of Nir operon. In this study, nitrite-resistant Lactobacillus plantarum WU14 was isolated from Pickles and its protease Nir was analyzed. GlnR-mediated regulation of L. plantarum WU14 Nir gene was investigated in this study. New GlnR and Nir genes were obtained from L. plantarum WU14. The regulation effect of GlnR on Nir gene was examined by gel block test, yeast two-hybrid system, bacterial single hybrid system and qRT-RCR. Detailed analysis showed that GlnR ound to the Nir promoter region and interacted with Nir at low nitrite concentrations, positively regulating the expression of NIR. However, the transcription levels of GlnR and Nir decreased gradually with increasing nitrite concentration. The results of this study improve our understanding of the function of the Nir operon regulatory system and serve as the ground for further study of the signal transduction pathway in lactic acid bacteria.

This study aimed to decolorize azo dyes in high-salt industrial wastewater under high-salt and low oxygen conditions using extreme halophilic/halotolerant bacteria screened from the salt fields of Tibet, which consisted of Enterococcus, unclassified Enterobacteriaceae, Staphylococcus, Bacillus, and Kosakonia. Under the optimal conditions, 600 mg/l Congo red, Direct Black G (DBG), Amaranth, methyl red, and methyl orange could be completely decolorized in 24, 8, 8, 12, and 12 h, respectively. When the DBG concentration was 600 mg/l, NADH–DCIP, laccase, and azo reductase were confirmed to be the primary reductase and oxidase during the degradation process, and the degradation pathways were verified. The microflora could not only tolerate changes in salt concentrations of 0–80 g/l, but also displayed strong degradative ability. Under high-salt concentrations (≥ 60 g/l NaCl), NADH–DCIP reductase was primarily used to decolorize the azo dye. However, under low salt concentrations (≤ 40 g/l NaCl), azo reductase began to function, and manganese peroxidase and lignin peroxidase could cooperate to participate in DBG degradation. Additionally, the halophilic/halophilic microflora was shown to convert the toxic DBG dye to metabolites of low toxicity based on phytotoxicity analysis, and a new mechanism for the microflora to degrade DBG was proposed based on intermediates identified by liquid chromatography-mass spectrometry (LC–MS). This study revealed that the halophilic/halophilic microflora has effective ecological and industrial value for treating wastewater from the textile industry.

Fiber-reinforced polymer (FRP) composites have been widely used to strengthen the existing reinforced concrete (RC) structures to against static and dynamic loads. During the past decades, the interfacial bond behavior between FRP and the concrete substrate under static load has been systematically investigated by experimental and numerical approaches. In contrast, the interfacial bond performance under dynamic loads, e.g., impact and explosive loading, is still far away from well known, especially taking the strain rate effect into account. In this contribution, the single-lap shear test is conducted to sixty specimens at the medium strain rate between 1.0E−4/s and 5.0E−3/s. The effects of various system parameters, including the strain rate, concrete strength, type of FRP and adhesive, on the interfacial fracture energy, peak shear stress, FRP strain distribution, interfacial shear stress, and effective bond length, are thoroughly investigated. It has been revealed that the strain rate and concrete strength can significantly affect the interfacial fracture energy and peak shear stress. The specimen with CFRP sheet possesses higher interfacial shear stress but lower fracture energy than that with BFRP sheet. The adhesive with lower elastic modulus is helpful to improve interfacial energy dissipation under dynamic load. The effective bond length decreases with concrete strength and strain rate, mainly between 75 mm and 90 mm, which is significantly shorter than that under static load. Inspired from the Kulkarni and Shah model, a new model is proposed to evaluate the interfacial fracture energy and peak shear stress with respect to the strain rate, and the estimated values agree well with the experiments.