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Surface coating is essential and critical to endow fiber materials with various functions for broad applications. However, it is still a great challenge to achieve a fast, fully covered, and robust surface coating on multiple fibers. In this work, a nanoscale surface coating with superior stability was rapidly and integrally formed on various fiber materials (such as Nylon mesh, nonwoven fabrics, and stainless-steel mesh) by highly reactive interfacial polymerization (IP) between polyethylenimine (PEI) and trimesoyl chloride (TMC). The resulting polyamide (PA) layer with an ultrathin thickness of tens of nanometers wholly and uniformly covered the surface of each fiber of the constituent material. Due to the synergistic effect of the PA layer with inherent robustness and the fully covered structure between the outer PA layer and the inner fiber, the nanosurface-coating exhibited outstanding mechanical stability, good acid resistance, and excellent organic solvent resistance. The functional modification of the nanosurface-coating can be easily carried out by using the abundant carboxyl groups in the PA layer. By introducing sulfobetaine zwitterionic copolymers via either "grafting from" or "grafting to" methods, the surfaces presented prominent underwater antioil-adhesion property and exceptional protein adhesion resistance. The surface coating based on IP process opens up an avenue in the field of surface modification. It is expected to offer a generally feasible strategy for the fabrication of fiber materials with robust and multifunctional coatings.

Acute myeloid leukemia (AML) is a common malignancy worldwide. Human immune deficiency virus type 1 enhancer-binding protein 3 (HIVEP3) was verified to play a vital role in types of cancers. However, the functional role of HIVEP3 in AML was rarely reported. In this study, CCK-8, colony formation assay, flow cytometry, and Trans-well chamber experiments were applied for detecting cell proliferation, apoptosis, and invasion in AML cells. The expression of proteins related to TGF-β/Smad signaling pathway was determined by western blot. Our data showed that the expression level of HIVEP3 was closely related to the risk classification and prognosis of AML patients. Moreover, HIVEP3 was highly expressed in AML patients and cells. Knockdown of HIVEP3 significantly repressed cell proliferation invasion, and enhanced cell apoptosis in HL-60 and THP-1 cells. In addition, HIVEP3 donwreglation could inhibit the TGF-β/Smad signaling pathway. TGF-β overexpression could reverse the inhibition effects of HIVEP3 knockdown on AML development and the TGF-β/Smad signaling pathway. These findings indicated that HIVEP3 contributed to the progression of AML

Improving the permeate flux but retaining the rejection of thin-film composite(TFC) polyamide nanofiltration(NF) membrane is a high requirement for desalination. In this work, a calcium ion(Ca2+) coordinated polyamide(PA) NF membrane was prepared by directly adding CaCl2 to the piperazine(PIP) aqueous solution during the interfacial polymerization process. Due to the coordination interaction between Ca2+ and the amide bond in PA active layer, the number of hydrogen bonds in the PA active layer was reduced, causing in turn the decrease of physical cross-linking degree. As a consequence, the pore of the PA active layer was enlarged, prominently enhancing the water permeance of NF membrane. With the increase of CaCl2 concentration, the pure water flux of TFC NF increased significantly while the rejection of Na2SO4 decreased sightly. Compared with TFC NF membrane prepared without CaCl2, the permeate flux of the Ca2+ coordinated polyamide NF membrane prepared under optimal conditions was increased by 3–4 folds with Na2SO4 rejection of 95.26%. Meanwhile, such a Ca2+ coordinated PA NF membrane showed a better SO42−/Cl− selectivity.

In mammals, the production of mature oocytes necessitates rigorous regulation of the discontinuous meiotic cell-cycle progression at both the transcriptional and post- transcriptional levels; however, the factors underlying this sophisticated but explicit process during oocyte development remain largely unclear. Here we characterized the function of N-acetyltransferase 10 (Nat10), which was previously recognized as a “writer” for N4-acetylcytidine (ac4C) deposited on RNA molecules. We generated two germline-specific Nat10 knockout mouse models in the embryonic gonad and in postnatal growing oocytes, and another two tamoxifen-inducible Nat10 deletion models. We provided genetic evidence showing that Nat10 is essential for oocyte meiotic prophase I progression, oocyte growth and maturation in mice. Intriguingly, we discovered that Nat10 is required for sculpting the maternal transcriptome through timely degradation of polyA tail mRNAs during the Maternal-to-Zygotic transition (MZT). Importantly, we developed a novel method that outperformed the conventional methods for examining the polyA tail length (PAT), termed Hairpin Adaptor-polyA tail length (HA-PAT), in terms of the cost, sensitivity and efficiency. In summary, these findings altogether provide solid genetic evidence that unveils the indispensable role of maternal Nat10 in oocyte development, and lay a solid foundation for future mechanistic studies of varied domains of NAT10.

Fabrication of nanofiltration (NF) membranes using interfacial polymerization (IP) continues to receive tremendous interest in research and development due to the broad applications of NF in water ...

Membranes with high permeance and high rejection for di- and multivalent cation removal are highly desired for efficient brackish water and industrial water treatment. In this work, we report a facile strategy for constructing ultrathin nanofiltration (NF) membranes by in situ cross-linking of amine which is confined in a network film. The network made of single-walled carbon nanotubes (SWCNTs) serves as a framework for poly(ethylene imine) (PEI) to attach and stay, facilitating the formation of a polyamine (PA) layer with high quality and controlled thickness. Benefiting from the ultrathin thickness of the SWCNT network (∼31 nm), an active layer (∼34 nm thick) comes with a high permeance of 27 L m

Porous polymeric support membranes with outstanding organic solvent resistance are highly required for fabricating thin-film composite (TFC) organic solvent nanofiltration (OSN) membranes. In this ...

Covalent organic framework (COF)-based membranes are burgeoning candidates for separation technologies owing to their well-ordered channel structures. The exponential interest in the stability of the COF membrane on exposure to harsh organic solvents is directed to develop a composite membrane for dye separations in polar aprotic solvents. Here, we reported a nanocomposite membrane composing of a single-walled carbon nanotube (SWCNT)/COF (an imine-based COF) hybrid on a commercial polytetrafluoroethylene (PTFE) substrate, with a thickness of ∼58 nm prepared in a diffusion cell. This membrane displayed high permeability and stability toward nonpolar and aprotic solvents. It exhibited high permeability for lower viscous organic solvents such as hexane (66 L m

The study of traditional Chinese medicines (TCMs) is receiving increasing attention worldwide because of their contribution to human health. Developing an effective and sustainable method for screening TCMs is highly desired to accelerate the modernization of TCMs. In this work, we report a neutrally charged membrane made of a positively charged polyelectrolyte electrostatically assembled on a negatively charged superhydrophilic nanoporous membrane. The composite membrane possesses stable electroneutrality in a wide pH range and can precisely and nonselectively separate various charged molecules in TCMs with a transmittance higher than 90% for molecules with molecular weight (Mw) 800. In addition, the membrane exhibits a superior antifouling performance, and the recovery ratio observed during a continuous cycling test of a simulated TCM solution was more than 93%. The combination of superhydrophilicity and electroneutrality in a nanoporous membrane provides a new route for designing nanofiltration membranes for highly efficient molecule separation and is promising for screening TCMs.

Despite the commercial success of thin film composite polyamide membranes, further improvements to the water permeation of polyamide membranes without degradation in product water quality remain a great challenge. Herein, we report the fabrication of an interfacially polymerized polyamide nanofiltration membrane with a novel 3D honeycomb-like spatial structure, which is formed from a tobacco mosaic virus (TMV) porous protein nanosheet-coated microfiltration membrane support. TMV nanosheets with uniform pores and appropriate hydrophilicity deposited inside the support membrane pores facilitate the construction of a localized water-oil reaction interface with evenly distributed monomers and guide the formation of a defect-free polyamide layer with a spatial structure that copies the geometry of the membrane cavities. Such a 3D morphology possesses ultrahigh specific surface area, leading to unprecedented membrane water permeance as high as 84 L m-2 h-1 bar-1, high MgSO4 rejection of 98%, and monovalent/divalent ion sieving selectivity up to 89.

Separating molecules or ions with sub-Angstrom scale precision is important but technically challenging. Achieving such a precise separation using membranes requires Angstrom scale pores with a high level of pore size uniformity. Herein, we demonstrate that precise solute-solute separation can be achieved using polyamide membranes formed via surfactant-assembly regulated interfacial polymerization (SARIP). The dynamic, self-assembled network of surfactants facilitates faster and more homogeneous diffusion of amine monomers across the water/hexane interface during interfacial polymerization, thereby forming a polyamide active layer with more uniform sub-nanometre pores compared to those formed via conventional interfacial polymerization. The polyamide membrane formed by SARIP exhibits highly size-dependent sieving of solutes, yielding a step-wise transition from low rejection to near-perfect rejection over a solute size range smaller than half Angstrom. SARIP represents an approach for the scalable fabrication of ultra-selective membranes with uniform nanopores for precise separation of ions and small solutes., Separating molecules or ions with sub-Angstrom scale precision is important but technically challenging. Here, the authors demonstrate that precise solute-solute separation can be achieved using polyamide membranes formed via surfactant-assembly regulated interfacial polymerization.

In this study, we report the emergence of two-dimensional (2D) branching fractal structures (BFS) in the nanoconfinement between the active and the support layer of a thin-film-composite polyamide (TFC-PA) nanofiltration membrane. These BFS are crystal dendrites of NaCl formed when salts are either added to the piperazine solution during the interfacial polymerization process or introduced to the nascently formed TFC-PA membrane before drying. The NaCl dosing concentration and the curing temperature have an impact on the size of the BFS but not on the fractal dimension (∼1.76). The BFS can be removed from the TFC-PA membranes by simply dissolving the crystal dendrites in deionized water, and the resulting TFC-PA membranes have substantially higher water fluxes (three- to fourfold) without compromised solute rejection. The flux enhancement is believed to be attributable to the distributed reduction in physical binding between the PA active layer and the support layer, caused by the exertion of crystallization pressure when the BFS formed. This reduced physical binding leads to an increase in the effective area for water transport, which, in turn, results in higher water flux. The BFS-templating method, which includes the interesting characteristics of 2D crystal dendrites, represents a facile, low-cost, and highly practical method of enhancing the performance of the TFC-PA nanofiltration membrane without having to alter the existing infrastructure of membrane fabrication.

Recently, ultrathin polyamide nanofiltration membranes fabricated on nanomaterial-based supports have overcome the limitations of conventional supports and show greatly improved separation performance. However, the feasibility of the nanomaterial-based supports for large-scale fabrication of the ultrathin polyamide membrane is still unclear. Herein, we report a controllable and saleable fabrication technique for a single-walled carbon nanotube (SWCNT) network support via brush painting. The mechanical and chemical stability of the SWCNT network support were carefully examined, and an ultrathin polyamide membrane with thickness of ∼15 nm was successfully fabricated based on such a support. The obtained thin-film composite (TFC) polyamide nanofiltration membranes exhibited extremely high water permeability of ∼40 L m-2 h -1 bar-1, a high Na2SO 4 rejection of 96.5%, and high monovalent/divalent ion permeation selectivity and maintained highly efficient ion sieving throughout 48 h of testing. This work demonstrates a practical route toward the controllable large-scale fabrication of the TFC membrane with an SWCNT network support for ion and molecule sieving. This work is also expected to boost the mass production and practical applications of state-of-the-art membranes composed of one-dimensional and two-dimensional nanomaterials as well as the nanomaterial-supported TFC membranes.

Deep desulfurization of fossil fuels has always been an urgent demand for environmental protection in fossil energy utilization. So, developing simple preparation method for adsorbents with high activity and low cost is always the goal of adsorption desulfurization. Cu-based materials are attractive π-complexation adsorbents owing to their high-cost performance. Here we report the fabrication of cuprous oxide-modified mesoporous carbon by a one-step thermal treatment at relatively low temperatures. In this method, the mesoporous carbon not only provides a stable platform but also acts as a reducing agent to promote the reaction of the Cu precursor. The impregnated Cu(NO3)2 precursor was transformed to the cuprous oxide by carbon-assisted reduction and decomposition at a much lower temperature (below 300 °C) without the requirement for an additional reducing agent. The resulting Cu2O-functionalized mesoporous carbon exhibited high surface area with 567 m2 g−1 and the benzothiophene capture ability reach 0.47 mmol g−1, which is significantly greater than those reported for Cu2O-modified mesoporous silicas. Furthermore, the majority of the adsorptive desulfurization of this material could be recovered over 90% after five recycling trials. This strategy has three advantages: one-step preparation at low temperatures, a high desulfurization capacity, and good reusability.

Objective: To investigate the prognostic significance of the platelet-to-lymphocyte ratio (PLR) for newborn patients in the neonatal intensive care unit (NICU).Design: A retrospective cohort study.Setting and participants: Data on 5240 newborn patients in the NICU extracted from the Multiparameter Intelligent Monitoring in Intensive Care III (MIMIC III) database.Methods: Spearman correlation was used to analyze the association of PLR with length of hospital and ICU stays. The chi-square test was used to analyze the association of PLR with mortality rate. Multivariable logistic regression was used to determine whether the PLR was an independent prognostic factor of mortality. The area under the receiver operating characteristic (ROC) curve was used to assess the predictive ability of models combining PLR with other variables.Results: PLR was negatively associated with length of hospital stay and ICU stay (hospital stay: Spearman’s rho=-0.416, PPPP=0.005) and 90-day mortality (OR=0.85, 95% CI=0.76-0.96, P=0.010). The prognostic predictive ability of models combining PLR with other variables for hospital mortality was moderately good (AUC for Model 1=0.804; AUC for Model 2=0.964).Conclusion: PLR is a novel independent risk factor for newborn patients in the NICU.

Thyroid cancer (TC) is the most common endocrine malignant disease with a rising morbidity year by year. Accumulating studies have shown that microRNAs (miRNAs) play a regulatory role in the progression of various tumors, but the molecular regulatory mechanism of miR-196a-2 in TC is still unknown. qRT-PCR was employed to measure the expression of miR-196a-2 and NRXN1 mRNA in TC cells, while western blot was used to detect the protein expression of NRXN1. CCK-8, colony formation and flow cytometry assays were used to measure cell proliferation and apoptosis of TC cells. Dual-luciferase reporter gene assay was used to predict and verify the targeted binding relationship between miR-196a-2 and NRXN1. Our study results manifested that miR-196a-2 was dramatically overexpressed in cells of TC, while NRXN1 was lowly expressed. miR-196a-2 could promote cell proliferation and inhibit cell apoptosis of TC. Additionally, miR-196a-2 could also target and inhibit the expression of NRXN1. Silencing NRXN1 could reverse the inhibitory effect of miR-196a-2 downregulation on cell proliferation of TC, as well as the promoting effect on cell apoptosis. In a conclusion, we found that miR-196a-2 could promote cell proliferation and inhibit cell apoptosis of TC by targeting NRXN1. Therefore, miR-196a-2/NRXN1 is potential to be a molecular therapeutic target for TC.

Objectives. Breast cancer is the most common malignant tumor among females, and miRNAs have been reported to play an important regulatory role in breast cancer progression. This study aimed to explore the function and underlying molecular mechanism of miR-301b-3p in breast cancer. Methods. Differential analysis and survival analysis were performed based on the data accessed from the TCGA-BRCA dataset for identification of the target miRNA. Bioinformatics analysis was conducted to predict the downstream target gene of the miRNA. Real-time quantitative PCR was carried out to detect the expression of miR-301b-3p and nuclear receptor subfamily 3 group C member 2 (NR3C2). Western blot was used to assess the protein expression of NR3C2. Cell counting kit-8 assay was performed to evaluate the proliferation of breast cancer cells. Transwell assay was conducted to determine the migratory and invasive abilities of breast cancer cells. Dual-luciferase reporter assay was employed to verify the targeting relationship between miR-301b-3p and NR3C2. Results. miR-301b-3p was elevated in breast cancer cell lines and promoted cell proliferation, migration, and invasion in terms of its biological function in breast cancer. NR3C2 was validated as a direct target of miR-301b-3p via bioinformatics analysis and dual-luciferase reporter assay, and NR3C2 was downregulated in breast cancer cell lines. The rescue experiment indicated that NR3C2 was involved in the mechanism by which miR-301b-3p regulated the malignant phenotype of breast cancer cells. Conclusion. The present study revealed for the first time that miR-301b-3p could foster breast cancer cell proliferation, migration, and invasion by targeting NR3C2, unveiling that miR-301b-3p is a novel carcinogen in breast cancer.

Summary It is of great importance and practical value to develop a facile and operable surface treatment method of materials with excellent antipollution and antiadhesion property, but still a huge challenge. In this work, a series of pseudo-zwitterions are prepared from electrostatic assembly of cationic polyethyleneimine and anionic phosphonic clusters. These pseudo-zwitterionic assemblies provide a strong hydration through electrostatic interaction with water and in turn create a barrier against oil foulants, leading to a nearly zero crude oil adhesion force. The pseudo-zwitterions-decorated surfaces exhibit exceptional water-cleanable oil-repellent property, even when they are completely dried and without prehydration before fouled by crude oil. While using these pseudo-zwitterions-modified polymeric membranes for separating surfactant stabilized oil-in-water emulsion, less than 10% decline of permeating flux is observed throughout a 2-h continuous separation experiment, showing excellent emulsion separation ability and antipollution performance for high viscous oil., Graphical abstract, Highlights • The ion pairs called pseudo-zwitterions were constructed by electrostatic self-assembly • The pseudo-zwitterions-modified surfaces can repel crude oil without prehydration • Pseudo-zwitterionic membrane shows exceptional antioil fouling performance • The strategy gives a new way to endow the surfaces with excellent antiadhesion property, Materials science; Materials chemistry; Materials synthesis; Materials property