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ObjectiveTo explore the clinical and genetic features of Aicardi-Goutières syndrome (AGS) caused by IFIH1 gene mutation.MethodsWe analyzed the clinical features and genetic mutation results of a boy with AGS type 7 and conducted a retrospective review of the literature of the characteristics and clinical features of IFIH1 gene mutations in AGS type 7.ResultsIn the case of this report, the patient, 13-year-old boy, exhibited gait abnormalities at age 3. As the condition was progressive, the boy has paraplegia of the lower limbs. The first brain MRI showed no lesions.Rehabilitation therapy in the past several years has shown no improvement.Recent brain CT revealed multiple intracranial calcifications. The whole-exome sequencing identified a heterozygous mutation in the IFIH1 gene (c.2159G > A, p.R720Q)- a known pathogenic mutation. Through review of the literature, we identified 69 cases of AGS type 7 (including the case reported here)which showed that skin and neurological system involvement are most commonly seen. Among these 69 patients, there were 30 different mutations in the IFIH1 gene, all of which are missense mutations. Seven patients had the same gene mutation as the boy in this study does, but their clinical features differed. In terms of treatment, Janus kainase(JAK) inhibitors are commonly used.Additionally, recent reports showed that tocilizumab treatment have been used for this condition.ConclusionsAGS7 is a type of I interferonopathy. Growth retardation and nervous system involvement are the most prevalent.The condition usually involve the skin, blood system, digestive system, kidney, heart, and other organs. JAK inhibitors prove effective for this disease.

C17H12FN3O, orthorhombic, Pbca (no. 61), a = 14.7608(7) Å, b = 7.2802(4) Å, c = 24.4842(13) Å, V = 2,631.1(2) Å3, Z = 8, Rgt(F) = 0.0900, wRref(F2) = 0.1841, T = 170 K.

Objective To establish a nomogram prediction model and analysis the risk factors for poor outcome (PO) in children with asthma combined with severe community-acquired pneumonia (As-SCAP) to provide personalized treatment references for clinics.Methods A total of 109 As-SCAP children diagnosed from September 2019 to September 2023 in the department of pediatrics of Qingdao Municipal Hospital were retrospectively collected and divided into PO and non-PO groups according to whether PO occurred during hospitalization. Use Logistic regression analysis to determine the independent risk factors for PO, and construct a nomogram predictive model based on the regression coefficients. Receiver operating characteristic curve (ROC), calibration curve and clinical decision curve were used to evaluate the predictive ability, calibration ability and clinical net benefit of the nomogram model.Results The incidence of PO was 27.52%. Asthma attacks in recent 3 months, psychiatric symptoms, septic shock, and haemoglobin (Hb)

In this article, we deal with the following pp-fractional Schrödinger-Kirchhoff equations with electromagnetic fields and the Hardy-Littlewood-Sobolev nonlinearity: M([u]s,Ap)(−Δ)p,Asu+V(x)∣u∣p−2u=λ∫RN∣u∣pμ,s*∣x−y∣μdy∣u∣pμ,s*−2u+k∣u∣q−2u,x∈RN,M({\left[u]}_{s,A}^{p}){\left(-\Delta )}_{p,A}^{s}u+V\left(x){| u| }^{p-2}u=\lambda \left(\mathop{\int }\limits_{{{\mathbb{R}}}^{N}}\frac{{| u| }^{{p}_{\mu ,s}^{* }}}{{| x-y| }^{\mu }}{\rm{d}}y\right){| u| }^{{p}_{\mu ,s}^{* }-2}u+k{| u| }^{q-2}u,\hspace{1em}x\in {{\mathbb{R}}}^{N}, where 0

Citrinin （CIT） is a mycotoxin produced by several species of the genera Aspergillus， Penicillium， and Monascus， and it often contaminates grains and grain products. CIT shows clear nephrotoxicity， while its carcinogenicity evidence is insufficient. Considering the ability of genotoxicity tests to screen chemicals for potential carcinogenicity， the genotoxicity of CIT was reviewed from the perspective of three genetic endpoints， including genetic mutation， chromosomal aberrations， and DNA damage. CIT is a genotoxic substance that induces chromosomal aberrations. The mechanism may be related to its influence on tubulin polymerization and spindle formation during cellular mitosis， thereby causing cell cycle blocking. This study provides basic data for follow-up risk assessments of CIT.

Objective: Taking Aksu apples as an example, a joint image optimal feature extraction and improved RBF neural network learning apple weight estimation method is designed to overcome the high cost and large error of manual grading and weighing. Methods: Firstly, an apple image acquisition system was established to obtain apple foreground image information. Secondly, the optimal subset extraction strategy for apple image feature sets was designed, by transforming the process of extracting the optimal subset into an objective function optimization problem, and an improved discrete locust optimization algorithm was designed to obtain the optimal apple image feature subset. Finally, a weight estimation model for apples based on RBF neural network learning was constructed, with the optimal feature subset as network input. The locust optimization algorithm was used to optimize the configuration of RBF neural network hyperparameters, to achieve effective estimation of apple weight. Results: The proposed apple weight estimation method had higher accuracy, with an average relative error rate of 1.23% for weight estimation. Conclusion: This method can effectively achieve apple weight estimation and can also be applied to other fruits with similar axisymmetric shapes for weight estimation.

Objective·To investigate if male alcohol-dependent patients have attention bias to alcohol-related pictures, show the behavioral and eye movement characteristics of attention bias, and explore the correlation between attention bias and sleep, anxiety, depression, impulsion and other psychological factors.Methods·Twenty-nine subjects in the case group (alcohol-dependent patients who were hospitalized in Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, from August 2021 to February 2022) and 29 healthy subjects in the healthy control group participated in the study. The experimental design was a mixed experimental design of three-factor hybrid. The cue types were alcohol-related and emotion pictures. The probe was on the same side or on the opposite side of the alcohol or emotional picture. The characteristics of subjects' attentional bias to alcohol cues were investigated by using the classical dot detection paradigm and eye movement monitoring technique. Groups were inter-group variables, and clue types and relative locations of probe points were intra-group variables. The behavioral and eye movement data were collected while the study subjects viewed pairs of alcohol-related and neutral pictures, or pairs of emotional and neutral pictures in a dot-probe paradigm. Dependent variables included behavioral indicators and eye movement indicators. Pittsburgh Sleep Quality Index (PSQI), 7-tiem Generalized Anxiety Disorder Scale (GAD-7), Patien Health Questionnaire (PHQ-9), and Barratt Impulsiveness Scale (BIS-11) scales were used to assess psychosomatic conditions such as sleep, anxiety, depression, and impulsivity, respectively. Behavioral and eye movement characteristics of attention bias in the alcohol-dependent group were analyzed. In addition, the correlation with behavioral and eye movement characteristics of attention bias and scale scores was analyzed.Results·The behavioral results showed that the reaction time of the alcohol-dependent group was significantly longer than that of the healthy control group (P=0.006). The eye movement results showed that when the type of cue was alcohol-related picture, the alcohol-dependent group had slower attention detection (P=0.031) and longer attention maintenance (P=0.005) on alcohol-related cues. Moreover, the pupil size during the viewing of alcohol-related cues (P=0.001) and emotional cues (P=0.008) were significantly smaller than those of the healthy control group. It was also found that, there was a negative correlation between the correct rate of the alcohol-dependent group and the BIS-11 scale score (r=-0.437, P=0.032), and a positive correlation between reaction time and GAD-7 score (r=0.407, P=0.033). The time of the first visual fixation entry was positively correlated with GAD-7 score (r=0.414, P=0.045), and the PSQI score was positively correlated with PHQ-9 score (r=0.422, P=0.041) in the case group.Conclusion·Alcohol-dependent patients have attention bias towards alcohol-related cues according to the behavioral and eye movement result. Compared with the traditional behavioral regression of attention bias based on correct rate and reaction time results, eye movement provides more direct and multi indicator evidence for the evaluation of attention bias in alcohol-dependent patients.

Dihydrochelerythrine (DHCHE) is an isoquinoline compound, which has distinct antifungal activity and can induce apoptosis. The antifungal activity of DHCHE against five rice pathogenic fungi was studied in vitro. At the concentration of 7.5 mg/L, DHCHE exhibited the highest efficacy among tested compounds in inhibiting mycelium growth, with an inhibition rate of 68.8% in Ustilaginoidea virens, which was approximately 2.4 times of that of validamycin (28.7%). After exposure to DHCHE, transmission electron micrographs revealed spores showed incomplete organelles, malformed cell walls and nuclear membranes, as well as irregular lipid spheres. Reactive oxygen species accumulation in treated spores was markedly higher than that in control spores. DHCHE induced cell damage increased in a dose-dependent manner, as indicated by the decrease in mitochondrial membrane potential and initiation of apoptosis. The differences of expression levels of Fip1, ACP1, PMS2 and COX13 that are important for oxidative phosphorylation and mismatch repair pathway were significant, which may be some of the reasons for the induction of apoptosis in DHCHE-treated U. virens. The protein levels of Fip1, ACP1, PMS2 and COX13 agreed with protein fold change ratio from parallel reaction monitoring Gene Ontology terms and Kyoto Encyclopedia of Genes and Genomes pathway of differentially expressed proteins were further analyzed. These findings will help to elucidate the mechanisms associated with antifungal and pro-apoptotic effects of DHCHE on U. virens, thereby aiding the potential development of novel pesticides.

As a hot spot in machine learning, graph neural networks (GNN) have recently begun to be applied in the field of fraud detection involving user reviews. In reality, the collected user comments involve diverse fields and complex information, and the fraud information in the massive user-generated content is usually in the minority, so that the GNN-based fraud detection methods are not ideal for this task. Aiming to solve the problems of heterogeneous features and uneven data distribution, a new ensemble hierarchical graph attention network (En-HGAN) detection method is proposed through modeling the review system as a heterogeneous network. The hierarchical attention is used to learn representations with richer semantics for comments by making full use of user behavior information in the heterogeneous network, and the Bagging framework introducing random under sampling is adopted to aggregate multiple discriminative HGAN sub-models, thereby reducing the effective information loss as well as enhancing the detection ability for fraud comments. Experimental results on YelpChi and Amazon real datasets show that this method has good anomaly detection performance. Compared with state-of-the- art methods, experimental results show that this method has nice robustness to deceptive entities when the data category is skewed.

ObjectiveTo provide scientific basis for the development and utilization of edible pigment Monascus red， the analysis of its key components and toxicological evaluation were carried out.MethodsIdentification test and physicochemical indexes test were carried out based on the samples according to the method in National food safety standard GB 1886.181—2016. The content of citrinin （CIT） and Monacolin K were detected through high performance liquid chromatography （HPLC）. UPLC-Orbitrap-MS2 were used for chemical composition and content determination， in which the molecular structure was also characterized. The 14-day oral toxicity test of SD rats was carried out， based on the acute oral toxicity test of Monascus red by the limited method.ResultsThe quality and specification of test samples conformed to the provisions of China National Food Safety Standard. The content of CIT and Monacolin K was 0.030 8 mg/kg （converted as one color value） and 0.166 mg/g. Twenty compounds were separated in Monascus red by UPLC-Orbitrap-MS2， red pigment， orange pigment and yellow pigment of it accounted for 88.38%， 2.04% and 5.96%， respectively. The results of acute oral toxicity showed that Monascus red was innocuous （LD50>20 g/kg·BW）. In the 14-day repeated oral dose toxicity test， compared with the control group， rats in 5 g/kg·BW dose group exhibited no significant differences in the general clinical observation， growth and development， hematology， blood biochemistry， routine urine detection， gross anatomy，organ weight，organ-to-body ratio， and histopathological examinations.ConclusionMonascus red is composed of multiple components characterized by red pigments. There is no obvious toxic effects found in the preliminary safety evaluation， which can provide reference for further long-term research.

Under the high-energy particle impingement and cascade effect, different types of radiation damage defects occur in metals. The aggregation and evolution of radiation damage defects can lead to the destruction of the internal structural stability and the deterioration of their properties of metals. Titanium alloy is a promising radiation resistant alloy due to its advantages of light weight, high strength, high temperature resistance and low radiation activity. Aiming at the improvement of the radiation damage resistance, this work summarizes the research progress on the radiation damage defects, the microstructural features and mechanical properties of titanium alloys. In addition, the formation and evolution of radiation damage defects and the influence mechanism of radiation dose, temperature and element species on defect migration and aggregation are analyzed. The microstructure evolution of titanium alloys induced by irradiation and the radiation damage effects such as radiation hardening, radiation embrittlement and radiation creep are discussed. The radiation damage resistance properties of titanium alloys are summarized and evaluated. The existing researches are lack effective methods to inhibit the radiation damage. Finally, the effective strategies to improve the radiation resistance of titanium alloys through composition regulation and interface microstructure design are prospected.

Objective To investigate the relationship between the treatment of EGFR-TKI icotinib and the prognosis of advanced lung adenocarcinoma patients with EGFR mutation. Methods Patients with advanced lung adenocarcinoma who had EGFR19 and 21 gene mutations and were treated with EGFR-TKI icotinib were enrolled. The relationships of clinical features, EGFR gene mutation subtypes, and different sites with patients'prognosis were analyzed. Results A total of 101 patients with advanced lung adenocarcinoma were included in this study, including 58 cases (57.4%) of EGFR gene exon 19 deletion mutation (EGFR Del19) and 43 cases (42.6%) of EGFR gene exon 21 point mutation (EGFR L858R). The objective response rate was 63.4%. The mPFS and mOS were 13 months and 27 months, respectively. In addition, the mPFS and mOS of EGFR Del19 and EGFR19 mutation 746-750 were higher than those of EGFR L858R and other EGFR mutations, respectively. Meanwhile, multivariate analysis showed that the number of metastatic sites and pleural metastasis were independent influencing factors of patients'OS (P=0.027; P=0.041). The mOS of patients with the number of metastatic sites ≤3 and without pleural metastasis were 29 and 27 months, respectively. Conclusion There is no significant difference found in overall survival of advanced lung adenocarcinoma patients treated with icotinib among different EGFR mutation subtypes and sites. Herein, the overall survival time is longer in patients with less than three metastatic sites and without pleural metastasis.

Mitogen-activated protein kinase (MAPK) cascades are conserved and universal signal transduction modules that play important roles in regulating stress responses in plants. Although MAP3K, MP2K, and MPK family in tea plant (Camellia sinensis) have been investigated, little is known about MPK family genes responding to various abiotic stresses in tea plant. In this study, we performed a comprehensive genome-wide analysis of the tea plant MAPKs (CsMPKs) family gene based on the genomic data of tea plants by bioinformatics-based methods. Here, 21 putative CsMPK genes were identified in the tea plant and divided into 4 subfamilies according to the homologous to Arabidopsis and their phylogenetic relationships. The gene structure and conserved motifs of these CsMPKs in the same group showed high similarity, suggesting that they were highly conserved and might have a similar function. The expression profiles of the CsMPK genes were further investigated by quantitative real-time reverse transcription PCR, indicating that many CsMPK genes were involved in response to cold, drought, heat, or heat combined with drought treatment, suggesting their potential roles in abiotic stress responses in tea plant. These results would provide valuable information for further exploring the functional characterization of CsMPK genes in tea plants.

Industrial Internet can drive the high-quality development of industries, and accelerating the development of industrial Internet is conducive to the transformation and upgrading of China’s manufacturing industry and to the improvement of its international competitiveness. To build an industrial Internet model that is universally adapted to large, medium, small, and micro enterprises, this study analyzes the existing industrial Internet models in China and abroad, and proposes an industrial Internet ecosystem model (IIEM) composed of three dimensions and six layers. Additionally, the operation mechanism of IIEM is presented in terms of entity, resource, digital intelligence, and dynamic value flows. Digital intelligence flow exhibits the flow value of data—a new production factor—at different levels and dimensions of IIEM, which explains the basic logic for improving the intelligent level of enterprise production systems and delivered products and expounds on the basic paths of digital transformation. IIEM emphasizes industrial attributes. Furthermore, the effectiveness of IIEM in assisting enterprises to understand the evolution and realization paths of industrial Internet and to integrate into the industrial Internet platform is verified through case study.

Objective To investigate the effect of circular RNA hsa_circ_0001922 on the proliferation, migration and invasion of prostate cancer cell PC-3 and its underlying molecular mechanism. Methods qRT-PCR and RNA FISH were used to detect the expression level and localization of hsa_circ_0001922 in PC-3 cells respectively. After hsa_circ_0001922 was targeted inhibited, clone formation, Transwell assay and scratch assay were used to detect the proliferation, migration and invasion abilities of PC-3 cells. qRT-PCR and Western blot were used to detect the expression levels of EMT pathway-related molecules after inhibiting hsa_circ_0001922. Results The expression of circular RNA hsa_circ_0001922 was increased in PC-3 cells (P < 0.01) and it existed in the cytoplasm and nucleus. The invasion, migration and invasion abilities were significantly weakened (P < 0.05) after inhibiting hsa_circ_0001922; the expression of E-cadherin mRNA increased (P < 0.05) while the mRNA level of Vimentin decreased (P < 0.05). The results of Western blot were consistent with the above (both P < 0.05). Conclusion The circular RNA hsa_circ_0001922 may promote the proliferation, migration and invasion of PC-3 cells through the EMT pathway.

Objective: This study aimed to explore the best parameters of buckwheat noodle composite preformed powder. Methods: Single factor experiment, multi-index comprehensive evaluation method and orthogonal experiment were used to optimize the special composite preformed powder recipe of buckwheat noodles. Results: The best parameters of buckwheat noodle composite preformed powder were 45% of buckwheat flour, 8% of modified starch, 1.2% of compound phosphate and 3% of gluten powder. In this situation, the tensile area of composite flour dough was 3 378.6 g·mm; the stabilization time was 7.23 min, the content of wet gluten was 23.71%; the sensory score was 89.2; the comprehensive score of multiple indexes was 0.926 5. Compared to the groups of single factor test and orthogonal test, the quality of composite powder in each test group was significantly improved (P＜0.05), and its application in non-fried instant noodles had a good effect. Conclusion: The optimized composite powder can be used as the raw powder of various kinds of noodles, such as buckwheat coarse grain noodles, semi-dry noodles and new-type non-fried coarse cake.

ObjectiveThe purpose of this study is to compare genotoxicity methods between OECD test guidelines and China food safety standards（GB15193）．MethodsMain parameters of related genotoxicity methods in OECD Testing Guidelines and GB 15193 standards were compared and analyzed．ResultsThe methodology system of genotoxicity tests was similar between OECD and GB15193. However， there were some different recommendations in some parameters including cells/animal selection， treatment concentrations/dose selection and evaluation of results， etc.ConclusionAs a set of internationally recognized standards， OECD test guidelines can be beneficial to the improvement of GB15193 standards on genotoxicity.

Aiming at the waveform of missile borne radar is simple and easy to be affected by the repeater jamming in the terminal guidance, an anti-jamming waveform optimization algorithm based on the frequency shift keying(FSK) and phase shift keying(PSK) composite modulation is proposed. The model function of FSK-PSK composite modulation signal is analyzed. Based on the orthogonality of sub pulses, an optimization algorithm of intra pulse PSK modulation based on the alternating direction multiplier method(ADMM) is designed. The simulation results show that comparing with PSK signal, the intra pulse sub pulse orthogonality of FSK-PSK signal optimized by modulation is improved by about 10 dB, which can effectively suppress the intra pulse forwarding interference.

Living heritage sites will have continuous changes in continuous use, and the general rule of their evolution has not been systematically studied. The evolution of living heritage sites is a continuous space production process. It is of great significance to reveal its characteristics and logic for the protection, utilization and inheritance of living heritage sites. Tradition, site and living are the three elements to express the liveness of heritage sites. The three elements are coupled with the "spatial ternary dialectics" (representation of space - space practice-space of representation) to build a TSL (Tradition-Site-Living) model. Applying this model to study Old Town of Lijiang, it is found that: 1) The Old Town has experienced three stages of space production, namely "consumption in space" around the intention of the ruling class under the guidance of political function, "production in space" around the needs of the citizen class under the guidance of industrial and commercial function, and "space production and consumption" centered on the activities of outside operators and tourists under the guidance of tourism function. Core communities have played a key role, and the reconstruction of core communities in different periods has a direct impact on the transformation of space production. 2) The change of external environment factors promotes the succession of the core use functions of the Old Town, triggering the interaction of living, site and tradition, so as to realize the space production process of each stage. The function adjustment is inseparable from the transformation of physical space, and the shaping of place will lead to the change of social life and the variation of cultural tradition. The power subject in social relations restricts the shaping of place and guides social life through traditional construction. The reconstruction of social relations can promote cultural exchanges and traditional evolution, and the transformation of lifestyle will promote the adjustment and optimization of physical space. 3) The continuous space production of living heritage sites is the result of the operation of endogenous mechanism driven by external factors, which follows three logics: the continuous change of time and space, the driving of external environmental factors and the operation of internal living elements. The logic of temporal and spatial continuous change shows that the living heritage sites are constantly changing in the continuous space production, but always inherit the historical and cultural value. The driving logic of external environmental factors shows that the change of external political and economic environment leads to the transformation of physical space, and the change of external social and cultural environment leads to the change of social structure, lifestyle and cultural representation. The operation logic of internal living elements shows that the ternary interaction of "living-site-tradition" is the operating mechanism of continuous space production of living heritage sites. The living heritage site is a space-time continuum composed of time, environment and space, and the TSL model provides a theoretical framework for revealing the basic rule of continuous changes in living heritage sites.

Power communication network (PCN) is the cornerstone of the stable operation of smart grid. The evaluation of the essential vulnerability of the PCN based on the node importance is advantageous to the protection of critical nodes and the improvement of the reliability and controllability of the system. So, firstly, this paper establishes a multi-index evaluation system for the analysis of important nodes. Based on the site operation level, site load capacity, network topology, service flow, service quality requirements and security operation requirements, the network characteristics of nodes are divided into four layers to describe. Meanwhile, the characteristics of node topology are subdivided into three dimensions to analyze. Then, fully considering the coupling between power network and communication network, a multi-dimensional and multi-layer node importance (MMNI) model based on entropy weight method is proposed. Combined the long-term and short-term vulnerability loss, the node cross-layer vulnerability loss (CVL) function and network cross-layer loss entropy (CVE) model are proposed. Finally, based on the actual power communication network, under different attacks and fault strategies, the changes of maximum network connectivity and network cross layer loss entropy are compared. It shows the effectiveness and accuracy of the MMNI model for the analysis and evaluation of important nodes and it provides a theoretical basis for key weak point identification and the network optimization.

In order to explore the engineering application potential of coal gangue-based geopolymer concrete (CGGPC), this article formulated the fiber-reinforced coal gangue-based geopolymer concrete (FRCGGPC). The mechanical properties of the specimens were tested to analyze the effects of different types and dosing amounts of fibers on the mechanical properties of CGGPC. The microscopic morphology of the specimens was observed by scanning electron microscopy (SEM) to analyze the strengthening mechanism of fibers on the mechanical properties of the CGGPC. The experimental results show that the splitting tensile strength of FRCGGPC can be improved obviously, but the improvement of compressive strength is not obvious. The control groups containing steel fiber (SF) and polyester fiber (PF) had peaks in their compressive strength. Under the same dosage, the effect of SF and PF on the splitting tensile strength of FRCGGPC is better than basalt fiber. The results of SEM analysis show that within a certain range of dosage, the fiber is closely combined with the matrix, which has a good force transmission effect and strengthens the matrix material. When the fiber dosage is too much, it is easy to agglomerate, which leads to the decrease in the mechanical properties of the specimens.

ObjectiveTo explore the relationship between schizotypal personality traits and creativity in college students and the mediating role of cognitive flexibility.MethodsSchizotypal Personality Questionnaire （SPQ）， Cognitive Flexibility Inventory （CFI） and Williams Creative Aptitude Test （WCAT） were used to assess 471 college students. Thereafter， Spearman correlation analysis was used to explore the relationship among the variables and the Bootstrap methodology was used to estimate the mediating role of cognitive flexibility.ResultsThe total SPQ， positive and disorganized schizotypal traits scores， and CFI score were all positively correlated with WCAT score （r=0.241~0.313， P

Geopolymer cementitious materials and recycled aggregate are typical representatives of material innovation research in the engineering field. In this study, we experimentally investigated a method to improve the performance of geopolymer-recycled aggregate concrete (GRAC). The recycled concrete aggregates and steel fiber (SF), fly ash (FA), metakaolin (MK), and sodium silicate solution were used as the main raw materials to prepare fiber-reinforced geopolymer-recycled aggregate concrete (FRGRAC). First, the orthogonal test was carried out to study the GRAC, and the optimal mix proportion was found. Second, building on the optimal mix proportion, the effects of the SF content on the slump, 7 and 28 days compressive strength, tensile strength, and flexural strength of FRGRAC were further studied. Finally, the microscopic mechanism of FRGRAC was studied by scanning electron microscopy (SEM). The study results indicate that the slump continues to decrease as the fiber content increases, but the compressive strength, tensile strength, and flexural strength increase to a certain extent. Through SEM analysis, it is found that SF restrains the development of cracks and improves the strength of concrete.

ObjectiveTo determine the incidence of fundus hemorrhage (FH) not associated with retinopathy of prematurity (ROP) during ocular screening and report their clinical features, risk factors, therapies, and prognosis in a large population of premature newborns.MethodsThe medical records of all preterm newborns admitted to the Department of Ophthalmology, Peking University People's Hospital, Beijing, from January 1, 2016 through August 31, 2021 were retrospectively reviewed. Fundus examinations were carried out by experienced retinal experts. Examination under anesthesia was carried out in newborns with abnormal fundus including vitreous hemorrhage (VH) or retinal hemorrhage (RH) >2 disks' diameter by a Retcam 2 system. A lens-preserving vitrectomy was performed in infants requiring a vitrectomy. A comprehensive medical history was also recorded and analyzed.ResultsDuring the 5-year period, a total of 7,260 preterm babies were screened. There were 82 (1.13%) newborns and 104 (0.72) eyes with FH, including VH or RH.Twelve (14.63%) newborns (16 eyes, 15.38%) had VH; 56 (68.29%) (74 eyes, 71.15%) had flame-shaped, superficial hemorrhages; six (7.31%) (6 eyes, 5.77%) had small, round, deep hemorrhages (2 disk diameters). In all, there were 10 (12.20%) cases of intracranial hemorrhage. The mode of delivery was not found to be a significant factor in the occurrence of birth-related retinal hemorrhage (P = 0.22).Six newborns (eyes) with large, round retinal hemorrhage at the posterior pole while the macular was not impacted and 11 cases (15 eyes) with vitreous hemorrhage were required to receive close follow-up with average follow-up time of 105 days. A lens-sparing vitreous surgery was conducted in three patients without any complications.ConclusionPreterm newborns with FH that are not caused by ROP are more likely to have superficial, peripheral hemorrhages. Vaginal delivery compression and forceps may be associated with hemorrhage. A lens-preserving vitrectomy is required and considered safe for dense FH involving the refractive media.

The problem of monitoring an electric power system by placing as few measurement devices in the system can be formulated as a power dominating set problem in graph theory. The power domination number of a graph is the minimum cardinality of a power dominating set. Xu and Kang [On the power domination number of the generalized Petersen graphs, J. Comb. Optim. 22 (2011) 282–291] study the exact power domination number for the generalized Petersen graph P (3k, k), and propose the following problem: determine the power domination number for the generalized Petersen graph P (4k, k) or P (ck, k). In this paper we give the power domination number for P (4k, k) and present a sharp upper bound on the power domination number for the generalized Petersen graph P (ck, k).

Background: The incidence and mortality rates of hepatocellular carcinoma (HCC) are increasing worldwide. Therefore, there is an urgent need to elucidate the molecular drivers of HCC for potential early diagnosis and individualized treatment. Whether c-Myc expression plays a role in the clinicopathology and prognosis of patients with HCC remains controversial. This meta-analysis aimed to survey the prognostic role of c-Myc in HCC.Methods: We searched PubMed, Cochrane Library, Embase, Web of Science, and Google Scholar databases for studies published through March 2020 that examined the association between c-Myc expression and clinicopathology or prognosis in HCC patients. The pooled hazard ratios (HRs) and 95% confidence intervals (CIs) were used to investigate the prognostic significance of c-Myc expression. Odds ratios were calculated to evaluate the association between c-Myc expression and clinicopathologic features. We also tested for publication bias.Results: Our meta-analysis included nine studies with 981 patients with HCC published between 1999 and 2016. A meta-analysis of these studies demonstrated that high c-Myc expression indicated a poor overall survival (OS) (HR = 2.260, 95% CI: 1.660–3.080, and p < 0.001) and disease-free survival (DFS) (HR = 1.770, 95% CI: 1.430–2.450, and p < 0.001) in patients with HCC. However, high c-Myc expression was not associated with HBsAg, pathological type, TNM stage, or cirrhosis. We did not find any significant publication bias among the included studies, indicating that our estimates were robust and reliable.Conclusion: c-Myc overexpression could predict poor OS and DFS in HCC patients. c-Myc could be a useful prognostic biomarker and therapeutic target for HCC.

Focusing on the energy efficiency (EE) improving problem in a densely deployed cellular network with partial conventional base stations (BSs) that cannot be frequently switched on/off, this paper jointly optimizes the switching on/off strategy and user association policy with the consideration of quality-of-service (QoS). By applying a modified switching cost model, we formulate the EE improving problem as an energy efficiency maximization problem (EEMP). To solve the EEMP efficiently, we first concentrate on the EEMP with a constant user distribution where switching cost is omitted temporarily, i.e., static- EEMP, and then the EEMP can be settled by using the results of the static-EEMP. After analyzing the hardness of solving the static-EEMP directly, several sub-optimal strategies are proposed for the static-EEMP to optimize the BSs working states and user association policy. With the results obtained from solving the static-EEMP, we propose a QoS First Maximum EE (QFMEE) scheme to solve the EEMP in two steps. In the first step, necessary BSs are switched on when the user distribution changes to ensure user QoS. In the second step, we judge the extra cost and the energy saving caused by the switching off operations to improve the network EE. Simulation results verify the effectiveness of our proposed strategies for static-EEMP and show that QFMEE can improve the network EE and reduce switching cost significantly when the switching cost occupies a considerable proportion in the total energy consumption.

A magnetic resonance (MR)-fluorescent dual-mode imaging probe was prepared by conjugating guaiazulene to Gd-DOTA, and used to label human mesenchymal stem cells (hMSCs) via electroporation. The probe self-assembled into nanoclusters in the cytoplasm, resulting in a significant reduction in T2-weighted signal intensity that persisted up to 7 days. The fluorescence signal of the probe was observed at 498 nm, and the labelled hMSCs emitted a green fluorescence. The MR-fluorescent dual-mode imaging probe can potentially be used for stem cell tracking in vivo.

BackgroundMicroglia play a critical role in neurodegenerative disorders, such as Alzheimer's disease, where alterations in microglial function may result in pathogenic amyloid-β (Aβ) accumulation, chronic neuroinflammation, and deleterious effects on neuronal function. However, studying these complex factors in vivo, where numerous confounding processes exist, is challenging, and until recently, in vitro models have not allowed sustained culture of critical cell types in the same culture.ObjectiveWe employed a rat primary tri-culture (neurons, astrocytes, and microglia) model and compared it to co-culture (neurons and astrocytes) and mono-culture (microglia) to study microglial function (i.e., motility and Aβ clearance) and proteomic response to exogenous Aβ.MethodsThe cultures were exposed to fluorescently-labeled Aβ (FITC-Aβ) particles for varying durations. Epifluorescence microscopy images were analyzed to quantify the number of FITC-Aβ particles and assess cytomorphological features. Cytokine profiles from conditioned media were obtained. Live-cell imaging was employed to extract microglia motility parameters.ResultsFITC-Aβ particles were more effectively cleared in the tri-culture compared to the co-culture. This was attributed to microglia engulfing FITC-Aβ particles, as confirmed via epifluorescence and confocal microscopy. FITC-Aβ treatment significantly increased microglia size, but had no significant effect on neuronal surface coverage or astrocyte size. Upon FITC-Aβ treatment, there was a significant increase in proinflammatory cytokines in tri-culture, but not in co-culture. Aβ treatment altered microglia motility evident as a swarming-like motion.ConclusionsThe results suggest that neuron-astrocyte-microglia interactions influence microglia function and highlight the utility of the tri-culture model for studies of neuroinflammation, neurodegeneration, and cell-cell communication.

Salmonella translocate to the gut epithelium via microfold cells lining the follicle-associated epithelium (FAE). How Salmonella localize to the FAE is not well characterized. Here we use live imaging and competitive assays between wild-type and chemotaxis-deficient mutants to show that Salmonella enterica serotype Typhimurium (S. Typhimurium) localize to the FAE independently of chemotaxis in an ex vivo mouse caecum infection model. Electrical recordings revealed polarized FAE with sustained outward current and small transepithelial potential, while the surrounding villus is depolarized with inward current and large transepithelial potential. The distinct electrical potentials attracted S. Typhimurium to the FAE while Escherichia coli (E. coli) localized to the villi, through a process called galvanotaxis. Chloride flux involving the cystic fibrosis transmembrane conductance regulator (CFTR) generated the ionic currents around the FAE. Pharmacological inhibition of CFTR decreased S. Typhimurium FAE localization but increased E. coli recruitment. Altogether, our findings demonstrate that bioelectric cues contribute to S. Typhimurium targeting of specific gut epithelial locations, with potential implications for other enteric bacterial infections.

This paper aims to understand the characteristics of domestic big data jobs requirements through k-means text clustering, help enterprises, and employees to identify big data talents, and promote the further development of big data-related research. Firstly, the crawler software is used to crawl the recruitment information about "big data" on the zhaopin.com recruitment website. Then, Jieba word segmentation and K-means text clustering are used to cluster big data recruitment positions, and the number of clustering was determined by the average sum of squares within the group. Finally, big data jobs are divided into 10 categories, and the urban distribution, salary level, education requirements, and experience requirements of big data jobs are discussed and analyzed from the perspectives of the overall data set and clustering results, to clarify the characteristics of big data job demands. The analysis results show that the job demands of big data are mainly distributed in first-tier cities and new first-tier cities. Enterprises are more inclined to job seekers with a college degree or bachelor’s degree and more than one year’s relevant experience. There are wage differences among different types of jobs. The higher the position, the higher the requirement for education and experience will be.

Recently, wireless sensor networks play an important role in our life. Researchers give more and more attention to the design of the routing algorithm. Cluster routing is the most popular routing protocol in wireless sensor networks. However, hotspot problem always exists. Unequal cluster routing is an effective method to solve this problem. In these protocols, nodes closer to base station have the smaller cluster size than other sensor nodes. It will reduce the relay pressure of the node which is near to the base station. The development of the software-defined network optimizes the performance of wireless sensor networks. In this article, we propose a software-defined and fuzzy-based multi-hop unequal cluster routing protocol. It adopts three methods to make the routing protocol outstanding. First, it uses a novel software-defined network architecture to reduce computation overhead and energy overhead in sensor nodes. Then, the fuzzy logic–based intracluster and intercluster routing is designed to balance energy consumption. Finally, residual energy–based rotating scheme is proposed to reduce most of the control overhead. According to the simulation results, the proposed protocol balances and reduces the energy consumption, extends the lifetime, and reduces the control overhead and latency of the network.