11 results on '"ZHICHUANG LIAN"'
Search Results
2. Home based pulmonary tele-rehabilitation under telemedicine system for COPD: a cohort study
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Ling Zhang, Ayiguli Maitinuer, Zhichuang Lian, Yafang Li, Wei Ding, Wenyi Wang, Chao Wu, and Xiaohong Yang
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Telemedicine ,Home based pulmonary rehabilitation ,Chronic obstructive pulmonary disease ,Effectiveness ,Diseases of the respiratory system ,RC705-779 - Abstract
Abstract Background Pulmonary tele-rehabilitation can improve adherence to pulmonary rehabilitation. However, there are few reports on home based pulmonary tele-rehabilitation. We assessed the effectiveness of home based pulmonary tele-rehabilitation under telemedicine system in patients with chronic obstructive pulmonary disease (COPD). Methods This cohort study enrolled 174 patients with COPD who received home based pulmonary tele-rehabilitation under telemedicine system. The follow-up time was 12 weeks. Patients were grouped according to pulmonary rehabilitation weeks, number of rehabilitation times and total duration time, and when these three data were inconsistent, the two lowest values were grouped: control group (total rehabilitation weeks 0.05). In the 12-week pulmonary rehabilitation program, patients who completed at least 8 weeks, namely those in the PR-3 and PR-4 groups, accounted for 42.5% of the total number. Education, income and response rate to telemedicine system reminders were the main risk factors associated with home based pulmonary tele-rehabilitation. Conclusions Home based pulmonary tele-rehabilitation under telemedicine system for more than 8 weeks can significantly improve the dyspnea symptoms, 6MWD, diaphragmatic mobility during deep breathing, and negative emotions of patients with moderate to severe stable COPD. Trial registration: This study was registered at Chinese Clinical Trial Registry under registration number of ChiCTR2200056241 CTR2200056241 .
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- 2022
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3. The Prediction Model of Risk Factors for COVID-19 Developing into Severe Illness Based on 1046 Patients with COVID-19
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Zhichuang Lian, Yafang Li, Wenyi Wang, Wei Ding, Zongxin Niu, Xiaohong Yang, and Chao Wu
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Medical emergencies. Critical care. Intensive care. First aid ,RC86-88.9 - Abstract
This study analyzed the risk factors for patients with COVID-19 developing severe illnesses and explored the value of applying the logistic model combined with ROC curve analysis to predict the risk of severe illnesses at COVID-19 patients’ admissions. The clinical data of 1046 COVID-19 patients admitted to a designated hospital in a certain city from July to September 2020 were retrospectively analyzed, the clinical characteristics of the patients were collected, and a multivariate unconditional logistic regression analysis was used to determine the risk factors for severe illnesses in COVID-19 patients during hospitalization. Based on the analysis results, a prediction model for severe conditions and the ROC curve were constructed, and the predictive value of the model was assessed. Logistic regression analysis showed that age (OR = 3.257, 95% CI 10.466–18.584), complications with chronic obstructive pulmonary disease (OR = 7.337, 95% CI 0.227–87.021), cough (OR = 5517, 95% CI 0.258–65.024), and venous thrombosis (OR = 7322, 95% CI 0.278–95.020) were risk factors for COVID-19 patients developing severe conditions during hospitalization. When complications were not taken into consideration, COVID-19 patients’ ages, number of diseases, and underlying diseases were risk factors influencing the development of severe illnesses. The ROC curve analysis results showed that the AUC that predicted the severity of COVID-19 patients at admission was 0.943, the optimal threshold was −3.24, and the specificity was 0.824, while the sensitivity was 0.827. The changes in the condition of severe COVID-19 patients are related to many factors such as age, clinical symptoms, and underlying diseases. This study has a certain value in predicting COVID-19 patients that develop from mild to severe conditions, and this prediction model is a useful tool in the quick prediction of the changes in patients’ conditions and providing early intervention for those with risk factors.
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- 2021
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4. B Lymphocytes Balance Th1/Th2 to Participate in Fibrotic Hypersensitivity Pneumonia Induced by Pigeon Shedding
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Chao Wu, Wei Ding, Yafang Li, Wenyi Wang, Zhichuang Lian, Xirennayi Muhataer, Yuan Zhou, Qifeng Li, and Xiaohong Yang
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Immunology ,Immunology and Allergy ,General Medicine - Abstract
Introduction: We investigated the molecular mechanism by which B lymphocytes regulate Th1/Th2 imbalance to participate in the pulmonary fibrosis in hypersensitivity pneumonia induced by pigeon shedding in rats. Methods: CD19+ rats and CD19− rats were used to construct animal models of fibrotic hypersensitivity pneumonia. DAPT was used to inhibit the Notch signaling pathway. The pathological changes were assessed with HE and Masson staining. Protein level was detected with Western blot. Th1/Th2 ratio was analyzed with flow cytometry. Cytokine levels were measured with ELISA. Results: The pathological changes of pulmonary fibrosis were not obvious in the CD19− rats and after DAPT treatment. Notch signaling pathway proteins, including Notch1, Notch2, Jag1, Jag2, DLL1, and DLL4, in lung tissues of model rats were all significantly upregulated than those in control rats. However, these proteins in CD19− rats were lower in CD19+ rats, suggesting that B cells play a key role in inducing pneumonia. Besides, the Th1/Th2 ratio in the BALF of model rats decreased, which was further reversed by DAPT. However, we found that in CD19− rats, the regulation of the Th1/Th2 ratio by the Notch signaling pathway was lost. Conclusion: Deleting B lymphocytes or blocking the Notch pathway both reversed the Th1/Th2 imbalance in fibrotic hypersensitivity pneumonia and inhibited pulmonary fibrosis.
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- 2022
5. Interaction between the PI3K/AKT pathway and mitochondrial autophagy in macrophages and the leukocyte count in rats with LPS-induced pulmonary infection
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Chao Wu, Lianghua Guo, Xirennayi Muhataer, Qifeng Li, Zhichuang Lian, Yafang Li, Wenyi Wang, Wei Ding, Yuan Zhou, Xiaohong Yang, and Muzhi Chen
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General Immunology and Microbiology ,General Neuroscience ,General Agricultural and Biological Sciences ,General Biochemistry, Genetics and Molecular Biology - Abstract
This study examined the effects of the PI3K/AKT pathway and mitochondrial autophagy in macrophages and the leukocyte count after pulmonary infection. Sprague‒Dawley rats were subjected to tracheal injection of lipopolysaccharide (LPS) to establish animal models of pulmonary infection. By inhibiting the PI3K/AKT pathway or inhibiting/inducing mitochondrial autophagy in macrophages, the severity of the pulmonary infection and the leukocyte count were altered. The PI3K/AKT inhibition group did not show a significant difference in leukocyte counts compared with the infection model group. Mitochondrial autophagy induction alleviated the pulmonary inflammatory response. The infection model group had significantly higher levels of LC3B, Beclin1, and p-mTOR than the control group. The AKT2 inhibitor group exhibited significantly increased levels of LC3B and Beclin1 compared with the control group (P < 0.05), and the Beclin1 level was significantly higher than that in the infection model group (P < 0.05). Compared with the infection model group, the mitochondrial autophagy inhibitor group exhibited significantly decreased levels of p-AKT2 and p-mTOR, whereas the levels of these proteins were significantly increased in the mitochondrial autophagy inducer group (P < 0.05). PI3K/AKT inhibition promoted mitochondrial autophagy in macrophages. Mitochondrial autophagy induction activated the downstream gene mTOR of the PI3K/AKT pathway, alleviated pulmonary inflammatory reactions, and decreased leukocyte counts.
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- 2023
6. Molecular mechanism by which the Notch signaling pathway regulates autophagy in a rat model of pulmonary fibrosis in pigeon breeder’s lung
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Yafang Li, Zhichuang Lian, Qifeng Li, Wei Ding, Wenyi Wang, Ling Zhang, Xirennayi Muhataer, Yuan Zhou, Xiaohong Yang, and Chao Wu
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General Medicine - Abstract
This study investigated the molecular mechanisms underlying the involvement of the Notch signaling pathway and autophagy in the development of pulmonary fibrosis in pigeon breeder’s lung (PBL). Rats were divided into control (Ctrl), PBL model (M), M + D (Notch signaling inhibition), M + W (autophagy inhibition), and M + R (autophagy induction) groups. Lyophilized protein powder from pigeon shedding materials was used as an allergen to construct a fibrotic PBL rat model. The mechanism by which Notch signaling regulated autophagy in the pulmonary fibrosis of PBL was investigated by inhibiting the Notch pathway and interfering with autophagy. Pulmonary interstitial fibrosis was significantly greater in the M group and the M + W group than in the M + D and M + R groups. The expression of α-smooth muscle actin was significantly higher in the M, M + D, and M + W groups than in the Ctrl group (P < 0.05). The expression of the cell autophagy markers Beclin1 and LC3 was lower in the M, M + D, and M + W groups than in the Ctrl group (P < 0.05), whereas Beclin1 and LC3 expressions were higher in the M + D and M + R groups than in the M group. The levels of reactive oxygen species in serum and lung tissues were higher in the M, M + D, M + W, and M + R groups than in the Ctrl group (P < 0.05). The Notch signaling pathway is involved in the pathological process of pulmonary fibrosis in the rat model of PBL by regulating autophagy.
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- 2023
7. The Prediction Model of Risk Factors for COVID-19 Developing into Severe Illness Based on 1046 Patients with COVID-19
- Author
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Xiaohong Yang, Chao Wu, Zhichuang Lian, Zongxin Niu, Wei Ding, Wenyi Wang, and Yafang Li
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medicine.medical_specialty ,Multivariate statistics ,Article Subject ,Coronavirus disease 2019 (COVID-19) ,RC86-88.9 ,business.industry ,Curve analysis ,Pulmonary disease ,Medical emergencies. Critical care. Intensive care. First aid ,medicine.disease ,Logistic regression ,Predictive value ,Venous thrombosis ,Internal medicine ,Emergency Medicine ,medicine ,In patient ,business ,Research Article - Abstract
This study analyzed the risk factors for patients with COVID-19 developing severe illnesses and explored the value of applying the logistic model combined with ROC curve analysis to predict the risk of severe illnesses at COVID-19 patients’ admissions. The clinical data of 1046 COVID-19 patients admitted to a designated hospital in a certain city from July to September 2020 were retrospectively analyzed, the clinical characteristics of the patients were collected, and a multivariate unconditional logistic regression analysis was used to determine the risk factors for severe illnesses in COVID-19 patients during hospitalization. Based on the analysis results, a prediction model for severe conditions and the ROC curve were constructed, and the predictive value of the model was assessed. Logistic regression analysis showed that age (OR = 3.257, 95% CI 10.466–18.584), complications with chronic obstructive pulmonary disease (OR = 7.337, 95% CI 0.227–87.021), cough (OR = 5517, 95% CI 0.258–65.024), and venous thrombosis (OR = 7322, 95% CI 0.278–95.020) were risk factors for COVID-19 patients developing severe conditions during hospitalization. When complications were not taken into consideration, COVID-19 patients’ ages, number of diseases, and underlying diseases were risk factors influencing the development of severe illnesses. The ROC curve analysis results showed that the AUC that predicted the severity of COVID-19 patients at admission was 0.943, the optimal threshold was −3.24, and the specificity was 0.824, while the sensitivity was 0.827. The changes in the condition of severe COVID-19 patients are related to many factors such as age, clinical symptoms, and underlying diseases. This study has a certain value in predicting COVID-19 patients that develop from mild to severe conditions, and this prediction model is a useful tool in the quick prediction of the changes in patients’ conditions and providing early intervention for those with risk factors.
- Published
- 2021
8. Establishment of Rat Models of Different Pathological Types of Hypersensitivity Pneumonitis Using Pigeon Droppings
- Author
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Zhichuang Lian, Xiaohong Yang, Yafang Li, Wei Ding, Zongxin Niu, Wenyi Wang, and Chao Wu
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Male ,Pathology ,medicine.medical_specialty ,Dander ,Pulmonary Fibrosis ,Immunology ,medicine.disease_cause ,Pathogenesis ,Rats, Sprague-Dawley ,Feces ,Allergen ,Bird Fancier's Lung ,Fibrosis ,Administration, Inhalation ,medicine ,Immunology and Allergy ,Animals ,Lymphocytes ,Columbidae ,Lung ,Aerosols ,Granuloma ,Inhalation ,business.industry ,Macrophages ,General Medicine ,Allergens ,Feathers ,medicine.disease ,Disease Models, Animal ,medicine.anatomical_structure ,Freeze Drying ,Female ,Powders ,business ,Hypersensitivity pneumonitis - Abstract
Background: The pathogenesis and pulmonary histopathological characteristics of hypersensitivity pneumonitis (HP) are not yet fully understood. Therefore, we established animal models of HP of different stages, aiming to provide support for research on this disease. Methods: We established rat models of pigeon breeder’s lung of different pathological types by creating freeze-dried allergen powder from fresh pigeon feathers, dander, and other droppings. Freeze-dried allergen powder suspensions of pigeon droppings were used to establish 2 rat models of HP, one by aerosol inhalation and one by airway instillation, and the rats were sacrificed after different lengths of time to observe the pathological changes in their lung tissues. Results: By the 40th week after allergen inhalation, granulomas were the main changes in the model, without fibrotic changes. When using airway instillation to establish the model, at the 20th week, group 1 (low dose + twice/week) and group 2 (medium dose + twice/week) showed granuloma changes, but no fibrosis; group 3 (high dose + once/week) and group 4 (high dose + twice/week) both showed obvious pulmonary fibrotic changes, but the death rate of rats in group 4 was greater. Conclusions: Both aerosol inhalation and airway instillation of freeze-dried pigeon allergen powder can successfully establish an HP model. The airway instillation method can cause pulmonary fibrotic changes in a short time, and the pulmonary pathological changes of animal models manifest with an obvious time-dose effect.
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- 2021
9. Abnormal DNA methylation patterns in patients with infection‑caused leukocytopenia based on methylation microarrays
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Dan Luo, Yafang Li, Xiaohong Yang, Wenyi Wang, Mingqin Deng, Rong Jin, Chao Wu, Zhichuang Lian, and Xirennayi Muhataer
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Adult ,Male ,Cancer Research ,Leukocytosis ,Ubiquitin-Protein Ligases ,leukocytopenia ,Severity of Illness Index ,Biochemistry ,Leukocyte Count ,Leukocytopenia ,gene chip technology ,Genetics ,medicine ,Humans ,Molecular Biology ,Aged ,Oligonucleotide Array Sequence Analysis ,Regulation of gene expression ,business.industry ,bacterial infection ,Case-control study ,Complement C5 ,Articles ,Complement C3 ,Leukopenia ,Methylation ,DNA Methylation ,Middle Aged ,Molecular biology ,Molecular medicine ,C-Reactive Protein ,Gene Ontology ,Oncology ,CpG site ,Case-Control Studies ,DNA methylation ,Molecular Medicine ,CpG Islands ,Female ,methylation ,medicine.symptom ,business ,WW domain containing E3 ubiquitin protein ligase 2 ,Procalcitonin - Abstract
The present study aimed to investigate the association between gene methylation and leukocytopenia from the perspective of gene regulation. A total of 30 patients confirmed as having post-infection leukocytopenia at People's Hospital of Xinjiang Uygur Autonomous Region between January 2016 and June 2017 were successively recruited as the leukocytopenia group; 30 patients with post-infection leukocytosis were enrolled as the leukocytosis group. In addition, 30 healthy volunteers who received a health examination at the hospital during the same period were included as the normal control group. In each group, four individuals were randomly selected for whole genome methylation screening. After selection of key methylation sites, the remaining samples in each group were used for verification using matrix-assisted laser desorption/ionization-time of flight mass spectrometry. The levels of serum complement factors C3 and C5 in the leukocytopenia group were significantly lower than those in the other two groups (P
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- 2020
10. Flexible and Compact MLWE-Based KEM
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Wenqi Liang, Zhaoman Liu, Xuyang Zhao, Yafang Yang, and Zhichuang Liang
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lattice-based cryptography ,post-quantum cryptography ,module learning with errors ,Kyber ,trinomial cyclotomics ,Mathematics ,QA1-939 - Abstract
In order to resist the security risks caused by quantum computing, post-quantum cryptography (PQC) has been a research focus. Constructing a key encapsulation mechanism (KEM) based on lattices is one of the promising PQC routines. The algebraically structured learning with errors (LWE) problem over power-of-two cyclotomics has been one of the most widely used hardness assumptions for lattice-based cryptographic schemes. However, power-of-two cyclotomic rings may be exploited in the inflexibility of selecting parameters. Recently, trinomial cyclotomic rings of the form Zq[x]/(xn−xn/2+1), where n=2k3l, k≥1,l≥0, have received widespread attention due to their flexible parameter selection. In this paper, we propose Tyber, a variant scheme of the NIST-standardized KEM candidate Kyber over trinomial cyclotomic rings. We provide three parameter sets, aiming at the quantum security of 128, 192, and 256 bits (actually achieving 129, 197, and 276 bits) with matching and negligible error probabilities. When compared to Kyber, our Tyber exhibits stronger quantum security, by 22, 31, and 44 bits, than Kyber for three security levels.
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- 2024
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11. Abnormal DNA methylation patterns in patients with infection-caused leukocytopenia based on methylation microarrays.
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CHAO WU, XIRENNAYI MUHATAER, WENYI WANG, MINGQIN DENG, RONG JIN, ZHICHUANG LIAN, DAN LUO, YAFANG LI, and XIAOHONG YANG
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DNA methylation ,METHYLATION ,UBIQUITINATION ,CHEMOTACTIC factors ,NAD (Coenzyme) ,GENETIC regulation ,PERIODIC health examinations ,UBIQUITIN ligases - Abstract
The present study aimed to investigate the association between gene methylation and leukocytopenia from the perspective of gene regulation. A total of 30 patients confirmed as having post-infection leukocytopenia at People's Hospital of Xinjiang Uygur Autonomous Region between January 2016 and June 2017 were successively recruited as the leukocytopenia group; 30 patients with post-infection leukocytosis were enrolled as the leukocytosis group. In addition, 30 healthy volunteers who received a health examination at the hospital during the same period were included as the normal control group. In each group, four individuals were randomly selected for whole genome methylation screening. After selection of key methylation sites, the remaining samples in each group were used for verification using matrix-assisted laser desorption/ionization-time of flight mass spectrometry. The levels of serum complement factors C3 and C5 in the leukocytopenia group were significantly lower than those in the other two groups (P<0.05). According to whole-genome DNA methylation detection, 66 and 27 methylation loci may be associated with leukocytopenia and leukocytosis, respectively. Most of these abnormal loci are located on chromosomes 2, 6, 7, 1, 17 and 11. The rates of WW domain containing E3 ubiquitin protein ligase 2 gene methylation at cytosine-phosphate-guanine (CpG)_1, CpG_5/6 and CpG_7 in the leukocytopenia group were higher than in the other two groups (P<0.05); the rate of AKT2 CpG_1 methylation was higher in the leukocytopenia group than in the other two groups (P<0.05); the rate of calcium-binding atopy-related autoantigen 1 gene CpG_2 methylation was higher in the leukocytosis group than in the normal control group (P<0.05); and the rate of NAD PH oxidase 5 gene CpG_3 methylation was higher in the leukocytosis group than in the normal control group (P<0.05). Chemotactic factor secretion and cell migration abnormalities, ubiquitination modification disorders and reduced oxidative burst may participate in infection-complicated leukocytopenia. The results of this study shed new light on the molecular biological mechanisms of infection-complicated leukocytopenia and provide novel avenues for diagnosis and treatment. [ABSTRACT FROM AUTHOR]
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- 2020
- Full Text
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