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17 results on '"Zacharias, Zeb R."'

4. Resident Synovial Macrophages in Synovial Fluid: Implications for Immunoregulation in Infectious and Inflammatory Arthritis

Author

Cyndari, Karen I., primary, Scorza, Breanna M., additional, Zacharias, Zeb R., additional, Strand, Leela, additional, Mahachi, Kurayi, additional, Oviedo, Juan Marcos, additional, Gibbs, Lisa, additional, Pessoa-Pereira, Danielle, additional, Ausdal, Graham, additional, Hendricks, Dylan, additional, Yahashiri, Rika, additional, Elkins, Jacob M., additional, Gulbrandsen, Trevor, additional, Peterson, Andrew R., additional, Willey, Michael C., additional, Fairfax, Keke C., additional, and Petersen, Christine A., additional

Published
2023
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5. OMIP‐099: 31‐color spectral flow cytometry panel to investigate the steady‐state phenotype of human T cells.

Author

Zacharias, Zeb R. and Houtman, Jon C. D.

Abstract

We have developed a 31‐color panel to define the steady‐state phenotype of T cells in human peripheral blood (Table 1). The panel presented here was optimized using cryopreserved peripheral blood mononuclear cells (PBMC). The markers included in this panel were chosen in order to characterize the steady‐state phenotype of T cells and includes markers (CD45RA, CD45RO, CCR7, CD95) to distinguish the main subsets (e.g., naïve, TEM, TCM, TEMRA, TSCM etc.) of CD4, CD8, and γδ T cells. This panel also includes markers for the identification of differentiation status (CD27, CD28), activation/antigen experience status (CD11a, CD49d, CD38, HLA‐DR, CD56, and CD39), co‐inhibitory marker expression (PD‐1, TIM‐3), and CD4 T helper subsets (CXCR3, CXCR5, CCR4, CCR6, Foxp3, CD25, and CD127). This optimized panel provides a broad assessment of the steady‐state phenotype of human T cells. [ABSTRACT FROM AUTHOR]

Published
2024
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6. Immunomodulatory Effects of Subacute Inhalation Exposure to Copper Oxide Nanoparticles in House Dust Mite-Induced Asthma

Author

Areecheewakul, Sudartip, primary, Adamcakova-Dodd, Andrea, additional, Zacharias, Zeb R., additional, Jing, Xuefang, additional, Meyerholz, David K., additional, Legge, Kevin L., additional, Houtman, Jon C. D., additional, O’Shaughnessy, Patrick T., additional, Thorne, Peter S., additional, and Salem, Aliasger K., additional

Published
2023
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7. Dendritic cell NLRC4 regulates influenza A virus-specific [CD4.sup.+] T cell responses through FasL expression

Author

Hornick, Emma E., Dagvadorj, Jargalsaikhan, Zacharias, Zeb R., Miller, Ann M., Langlois, Ryan A., Chen, Peter, Legge, Kevin L., Bishop, Gail A., Sutterwala, Fayyaz S., and Cassel, Suzanne L.

Subjects
Cell death -- Comparative analysis -- Health aspects, Influenza -- Comparative analysis -- Health aspects, Dendritic cells -- Comparative analysis -- Health aspects, T cells -- Comparative analysis -- Health aspects, Virus diseases, Infection, Health care industry
Abstract

Influenza A virus-specific (IAV-specific) T cell responses are important correlates of protection during primary and subsequent infections. The generation and maintenance of robust IAV-specific T cell responses relies on T cell interactions with dendritic cells (DCs). In this study, we explore the role of the nucleotide-binding domain leucine-rich repeat- containing receptor family member NLRC4 in modulating the DC phenotype during IAV infection. [Nlrc4.sup.-/-] mice had worsened survival and increased viral titers during infection, normal innate immune cell recruitment, and IAV-specific [CD8.sup.+] T cell responses, but severely blunted IAV-specific [CD4.sup.+] T cell responses compared with WT mice. The defect in the pulmonary IAV-specific [CD4.sup.+] T cell response was not a result of defective priming or migration of these cells in [Nlrc4.sup.-/-] mice but was instead due to an increase in [FasL.sup.+] DCs, resulting in IAV- specific [CD4.sup.+] T cell death. Together, our data support a role for NLRC4 in regulating the phenotype of lung DCs during a respiratory viral infection and thereby influencing the magnitude of protective T cell responses., Introduction Influenza A virus (IAV) is a respiratory pathogen responsible for seasonal epidemics that can cause severe disease and death, most commonly in the very young, very old, and immunocompromised [...]

Published
2019
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9. Pharmacological ascorbate as a novel therapeutic strategy to enhance cancer immunotherapy

Author

Zaher, Amira, primary, Stephens, Laura M., additional, Miller, Ann M., additional, Hartwig, Stacey M., additional, Stolwijk, Jeffrey M., additional, Petronek, Michael S., additional, Zacharias, Zeb R., additional, Wadas, Thaddeus J., additional, Monga, Varun, additional, Cullen, Joseph J., additional, Furqan, Muhammad, additional, Houtman, Jon C. D., additional, Varga, Steven M., additional, Spitz, Douglas R., additional, and Allen, Bryan G., additional

Published
2022
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17. Resident Synovial Macrophages in Synovial Fluid: Implications for Immunoregulation in Infectious and Inflammatory Arthritis.

Author

Cyndari KI, Scorza BM, Zacharias ZR, Strand L, Mahachi K, Oviedo JM, Gibbs L, Pessoa-Pereira D, Ausdal G, Hendricks D, Yahashiri R, Elkins JM, Gulbrandsen T, Peterson AR, Willey MC, Fairfax KC, and Petersen CA

Abstract

Objectives: Resident synovial macrophages (RSM) provide immune sequestration of the joint space and are likely involved in initiation and perpetuation of the joint-specific immune response. We sought to identify RSM in synovial fluid (SF) and demonstrate migratory ability, in additional to functional changes that may perpetuate a chronic inflammatory response within joint spaces., Methods: We recruited human patients presenting with undifferentiated arthritis in multiple clinical settings. We used flow cytometry to identify mononuclear cells in peripheral blood and SF. We used a novel transwell migration assay with human ex-vivo synovium obtained intra-operatively to validate flow cytometry findings. We used single cell RNA-sequencing (scRNA-seq) to further identify macrophage/monocyte subsets. ELISA was used to evaluate the bone-resorption potential of SF., Results: We were able to identify a rare population of CD14 dim , OPG + , ZO-1 + cells consistent with RSM in SF via flow cytometry. These cells were relatively enriched in the SF during infectious processes, but absolutely decreased compared to healthy controls. Similar putative RSM were identified using ex vivo migration assays when MCP-1 and LPS were used as migratory stimulus. scRNA-seq revealed a population consistent with RSM transcriptionally related to CD56 + cytotoxic dendritic cells and IDO + M2 macrophages., Conclusion: We identified a rare cell population consistent with RSM, indicating these cells are likely migratory and able to initiate or coordinate both acute (septic) or chronic (autoimmune or inflammatory) arthritis. RSM analysis via scRNA-seq indicated these cells are M2 skewed, capable of antigen presentation, and have consistent functions in both septic and inflammatory arthritis.

Published
2024
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