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Abstract Flavonoids are compounds that result from the secondary metabolism of plants and play a crucial role in plant development and mitigating biotic and abiotic stresses. The highest levels of flavonoids are found in legumes such as soybean. Breeding programs aim to increase desirable traits, such as higher flavonoid contents and vigorous seeds. Soybeans are one of the richest sources of protein in the plant kingdom and the main source of flavonoid derivatives for human health. In view of this, the hypothesis of this study is based on the possibility that the concentration of isoflavones in soybean seeds contributes to the physiological quality of the seeds. The aim of this study was to analyze the content of flavonoids in soybean genotypes and their influence on the physiological quality of the seeds. Seeds from thirty-two soybean genotypes were obtained by carrying out a field experiment during the 2021/22 crop season. The experimental design was randomized blocks with four replications and thirty-two F3 soybean populations. The seeds obtained were subjected to germination, first germination counting, electrical conductivity and tetrazolium vigor and viability tests. After drying and milling the material from each genotype, liquid chromatography analysis was carried out to obtain flavonoids, performed at UPLC level. Data were submitted to analysis of variance and, when significant, the means were compared using the Scott-Knott test at 5% probability. The results found here show the occurrence of genotypes with higher amounts of flavonoids when compared to their peers. The flavonoid FLVD_G2 had the highest concentration and differed from the others. Thus, we can assume that the type and concentration of flavonoids does not influence the physiological quality of seeds from different soybean genotypes, but it does indirectly contribute to viability and vigor, since the genotypes with the highest FLVD_G2 levels had better FGC values. The findings indicate that there is a difference between the content of flavonoids in soybean genotypes, with a higher content of genistein. The content of flavonoids does not influence the physiological quality of seeds, but contributes to increasing viability and vigor.

ABSTRACT Cobalt (Co) and molybdenum (Mo) are essential elements with a fundamental role in biological nitrogen fixation in legumes. Vitamins such as thiamine, despite being required in small quantities, influence plant growth. This study aimed to assess the efficiency of different doses of thiamine applied as seed treatment, combined or not with Co and Mo, in enhancing the development of soybean crops. The experiment was conducted in a greenhouse according to a randomized block design with a 6 × 2 factorial arrangement, comprising six thiamine doses (0, 10, 25, 50, 100, and 200 mg kg-1) in the presence or absence of Co and Mo at the recommended rate of 100 mL ha-1. At 40 days after sowing, plants were analyzed for root length, root dry weight, shoot length, shoot dry weight, nodule number, and nodule dry weight. Principal component analysis showed that combined application of Co and Mo with thiamine was negatively associated with all variables and that the thiamine doses most positively associated with the analyzed variables were 50 and 200 mg kg-1. In treatments containing thiamine alone, there was a linear direct relationship between thiamine dose and nodule dry weight. Root and shoot lengths and dry weights were highest in plants treated with thiamine only at a dose of 122 mg kg-1. Application of Co and Mo combined with thiamine via seed treatment does not promote the development of soybean crops. Thiamine application is a promising treatment to increase shoot length, root dry weight, and nodule dry weight in soybean.

Significant advancements have been achieved in delineating the progress of the Global PROMS (PROMS) Initiative. The PROMS Initiative, a collaborative endeavor by the European Charcot Foundation and the Multiple Sclerosis International Federation, strives to amplify the influence of patient input on MS care and establish a cohesive perspective on Patient-Reported Outcomes (PROs) for diverse stakeholders. This initiative has established an expansive, participatory governance framework launching four dedicated working groups that have made substantive contributions to research, clinical management, eHealth, and healthcare system reform. The initiative prioritizes the global integration of patient (For the purposes of the Global PROMS Initiative, the term “patient” refers to the people with the disease (aka People with Multiple Sclerosis – pwMS): any individual with lived experience of the disease. People affected by the disease/Multiple Sclerosis: any individual or group that is affected by the disease: E.g., family members, caregivers will be also engaged as the other stakeholders in the initiative). insights into the management of MS care. It merges subjective PROs with objective clinical metrics, thereby addressing the complex variability of disease presentation and progression. Following the completion of its second phase, the initiative aims to help increasing the uptake of eHealth tools and passive PROs within research and clinical settings, affirming its unwavering dedication to the progressive refinement of MS care. Looking forward, the initiative is poised to continue enhancing global surveys, rethinking to the relevant statistical approaches in clinical trials, and cultivating a unified stance among ‘industry’, regulatory bodies and health policy making regarding the application of PROs in MS healthcare strategies.

Abstract As patient-advocacy, public policy and clinical researchers with special knowledge on Responsible Research Innovation (RRI) governance and the public health and psychology underlying patient engagement, we read with interest the comment contribution by Richards et al., “Reflections on patient engagement by patient partners: How it can go wrong” (Richards et al. in Res Involv Engagem 9:41, 2023. https://doi.org/10.1186/s40900-023-00454-13 ). As a way to help meet the “take-away actions for readers” included by the authors at the end of the article, we would like to further stimulate discussion with relevant stakeholder communities about the need to rethink the use of “expert patient”. Based on our experience, the lack of a governance model engaging patients who are representative of the target patient community, as opposed to expert patients, is at the root of the tokenistic approach, the “patient partner as a checkmark statement” and the “lack of recognizing the vulnerability of patient partners”, which results in “patient engagement going wrong”. According to our experience, the Responsible Research Innovation (RRI) MULTI-ACT model has the potential to help meet these challenges.

ABSTRACT This study investigated the effectiveness of using the X-ray technique to assess the viability of papaya seeds. The X-ray images were performed in eight replicates of 25 seeds, placed on transparent acrylic plates on double-sided adhesive tape, previously identified, and then submitted to radiation in X-ray equipment "HP Faxitron". After exposure to X-ray radiation, the seeds were submitted to the germination test in rolls of germitest paper and kept in a germination chamber at 20-30 °C for 30 days. Seedlings were photographed and evaluated as normal seedlings, abnormal and non-germinated seeds. Afterward, the X-ray images and the seedlings from the same seeds were visually compared, and the X-ray images clearly showed seeds with and without internal filling. All abnormal seedlings were full seeds; therefore, X-ray images were not able to separate normal and abnormal. A 100% accuracy was verified by comparing the X-ray images and their respective seedlings, full seeds originated normal or abnormal seedlings, and empty seeds corresponded to non-germinated seeds. Therefore, using X-ray images is a promising technique to identify the viability of papaya seeds. The automated analysis of X-ray images is a simple, fast, and efficient technique to provide information on seed viability and generate parameters related to their germination capacity.

Abstract: Melon is one of the most consumed cucurbits and commercially significant cucurbits in the world. It is planted predominantly through seedlings, and seed quality is essential in this process. Among the rapid tests used in seed analysis, the pH testing of exudate has shown promise for several cultivated species. Thus, this study aimed to verify the viability of melon (Cucumis melo L.) seeds through the pH testing of exudate. For this, seeds of the hybrids Premier and Supreme (three lots each) were subjected to germination and vigor tests (first germination count, tetrazolium, accelerated aging, emergence speed index, and emergence). The pH testing of exudate was evaluated using phenolphthalein and sodium carbonate solutions. The results indicated the possibility of classifying the lots into three quality levels for both hybrids, similar to most tests used to assess initial quality. Therefore, the pH testing of exudate was effective in determining the viability of lots of melon seeds and can be conducted with a soaking period of 120 minutes at 25 or 30 °C.

The research study aimed at providing an accurate low-dose benzene exposure assessment method, by validating diffusive monitoring techniques for benzene personal exposure measurements at workplaces where benzene concentrations are expected in the low ppb range, such as in the present-day chemical, petrochemical, foundry, and pharmaceutical industry. The project was aimed at addressing the need for a robust and fully validated method to perform personal exposure measurements considering that the occupational exposure limit value for benzene is going to be significantly lowered in the next few years. Diffusive sampling offers a reliable alternative to pumped sampling methods, intrinsic safety in potentially explosive atmospheres, lightness, and ease of use. In this study, the radiello® diffusive sampler, with the packed activated charcoal RAD130 adsorbing substrate [suitable for solvent desorption and analysis by high-resolution gas chromatography-flame ionization detection (HRGC-FID)], was used. The experiments have been conducted following the ISO 23320 standard in the range from 0.005 to 0.1 ppm (16 to 320 μg/m3), yielding a full validation of the sampling and analytical method. The sampler performances have fulfilled all requisites of the ISO 23320 standard, in particular: bias due to the selection of a non-ideal sorbent is lower than 10% (no significant back diffusion of benzene due to concentration change in the atmosphere); bias due to storage of samples for up to 2 months is lower than 10%; nominal uptake rate for benzene on RAD130 is 74.65 mL/min; and expanded uncertainty of the sampling and analytical method is 20.6%. The sampling and analytical method is therefore fit-for-purpose for the personal exposure measurements aimed at testing compliance with occupational exposure limit values for benzene. The method is also fit for short-duration exposure monitoring related to specific tasks, and other volatile organic compounds, usually found in the same workplaces, such as aliphatic and aromatic hydrocarbons and some oxygenated compounds, have also been studied. In particular, n-hexane and isopropyl benzene, whose classification is currently under revision, can be efficiently monitored by this technique.

ABSTRACT Harvest delay can alter the physiological and sanitary quality of sorghum seeds due to the longer exposure time of seeds to adverse environmental conditions in the field. In this study, we investigated the impact of delayed Sorghum sp. harvest on the physiological and sanitary quality of seeds in four hybrids grown in the tropical soil of the Brazilian Cerrado region. Plants from four Sorghum sp. hybrids (IPA SUDAN 4202, BRS 658, BRS 810, and BRS 373) were grown and submitted to four seed harvest periods (0, 7, 14, and 21 days after stage 9 - seed physiological maturity). The treatments were arranged in a completely randomized block design in a 4 × 4 factorial, with four replicates. At each harvest period, the thousand-seed weight, water content, first count of the germination test, germination, seedling emergence, emergence speed index, seedling dry matter, accelerated aging, electrical conductivity, and sanitary quality of seeds were determined. Seeds of higher physiological and sanitary quality of the IPA SUDAN 4202 and BRS 658 sorghum hybrids can be obtained when the harvest is carried out up to 7 days after the physiological maturity stage. However, the delay in seed harvesting at 14 and 21 days after physiological maturity results in a higher incidence of Colletotrichum sp., Curvularia sp., Rhizoctonia sp., and Alternaria sp. on the seeds of these hybrids. Delaying the harvest of sorghum seeds from 7 days after the physiological maturity stage compromises seed vigor and viability and increases the incidence of pathogens.

ABSTRACT Stress caused by defoliation can compromise the physiological quality of soybean seeds. However, there is evidence that the application of nitrogen (N) can be an alternative to reduce the qualitative losses in seeds caused by defoliating insects. The objective of this study was to evaluate the effectiveness of topdressing N fertilization in improving the physiological quality of soybean seeds from plants subjected to artificial defoliation. The experimental design was a randomized block arranged in a 2×4 factorial scheme with four replicates. The treatments consisted of two levels of defoliation (33 and 66%) and four doses of N (0, 50, 100, and 150 kg ha-1), using urea (45% N), applied two days after defoliation. Artificial defoliation was performed during the R3 stage by removing one (33%) and two leaflets (66%) from each trifoliate leaf under field conditions. At stage R5.1, leaf area was quantified, and after harvesting, germination, emergence, length, total dry mass, electrical conductivity, total protein content, mass of 1000 seeds, and tetrazolium (vigor and viability) tests were carried out. It was concluded that increasing the defoliation from 33 to 66% impaired the physiological quality of soybean seeds. The use of nitrogen fertilization for coverage after defoliation at the beginning of the reproductive phase cannot minimize qualitative losses in seeds.

ObjectiveThis study was undertaken to assess the impact of immunosuppressive and immunomodulatory therapies on the severity of coronavirus disease 2019 (COVID-19) in people with multiple sclerosis (PwMS).MethodsWe retrospectively collected data of PwMS with suspected or confirmed COVID-19. All the patients had complete follow-up to death or recovery. Severe COVID-19 was defined by a 3-level variable: mild disease not requiring hospitalization versus pneumonia or hospitalization versus intensive care unit (ICU) admission or death. We evaluated baseline characteristics and MS therapies associated with severe COVID-19 by multivariate and propensity score (PS)-weighted ordinal logistic models. Sensitivity analyses were run to confirm the results.ResultsOf 844 PwMS with suspected (n = 565) or confirmed (n = 279) COVID-19, 13 (1.54%) died; 11 of them were in a progressive MS phase, and 8 were without any therapy. Thirty-eight (4.5%) were admitted to an ICU; 99 (11.7%) had radiologically documented pneumonia; 96 (11.4%) were hospitalized. After adjusting for region, age, sex, progressive MS course, Expanded Disability Status Scale, disease duration, body mass index, comorbidities, and recent methylprednisolone use, therapy with an anti-CD20 agent (ocrelizumab or rituximab) was significantly associated (odds ratio [OR] = 2.37, 95% confidence interval [CI] = 1.18-4.74, p = 0.015) with increased risk of severe COVID-19. Recent use (

ABSTRACT The objective was to verify if the primary root emission can be recommended as a vigor test for soybean seeds. For this, five lots of varieties ANTA 82, NA7337 and BRS1074IPRO were evaluated by first count, germination, emergence, emergence speed index and tetrazolium tests. In the root protrusion test at 20, 25 and 30 ºC, two evaluation criteria were adopted: primary root emission precocity index (PREPI) and percentage of seeds that emitted the primary root (PRE). The seeds counted were those with a primary root equal to or greater than 2 mm, every 12 h from the installation of the test until 120 h. The design was completely randomized and means were compared, within each evaluation time, using the Scott-Knott test at 5% probability. Pearson’s linear correlation was performed for PREPI and PRE with emergence. PREPI was not considered efficient. For the PRE, at 20 ºC, there was a correlation at 72 h, for the three varieties. At 25 ºC there was a correlation for a period of 36 h for ANTA 82 and NA7337 and 60 h for BRS1074IPRO. At 30 ºC there was a correlation in the time of 36 h for the three cultivars. Considering that speed in obtaining results is important in vigor tests, it is concluded that primary root emission for 36 h after installing the test at 30 ºC is recommended as a vigor test in the quality control of soybean seeds.

ABSTRACT Among the main problems that compromise the development of forest species are water and salt stresses, especially in the early stages of development, when seedlings are more sensitive. Thus, the objective of this study was to evaluate the morphophysiological and biochemical changes in Enterolobium contortisiliquum seedlings subjected to abiotic stresses. The experimental design was randomized complete blocks (RCB), composed of five treatments, with four replicates, and the experimental plot consisted of twenty plants. From the 31st day after sowing, when the seedlings reached approximately 15 cm in height, they began to receive the treatments, which consisted of different levels of electrical conductivity of irrigation water (0.3; 2; 4; 6; 8 dS m-1) for salt stress and periods of water restriction (0; 4; 8; 12 and 16 days without irrigation) for water stress. The traits evaluated were: plant height, root length, stem diameter, number of leaves, leaf area, total dry matter, Dickson quality index, height/shoot dry matter ratio, total soluble sugars, starch, total amino acids and proline. An increase in the electrical conductivity of irrigation water and days without irrigation compromised the development of E. contortisiliquum seedlings, which was intensified from 2 dS m-1 and four days without irrigation for salt and water stresses, respectively. The mechanism of survival of E. contortisiliquum to abiotic stresses involves reduction in the growth and quality of seedlings, with activation of biochemical defense mechanisms of the species.

Abstract Background The need to more collaboratively measure the impact of health research and to do so from multidimensional perspectives has been acknowledged. A scorecard was developed as part of the Collective Research Impact Framework (CRIF), to engage stakeholders in the assessment of the impacts of health research and innovations. The purpose of this study was to describe the developmental process of the MULTI-ACT Master Scorecard (MSC) and how it can be used as a workable tool for collectively assessing future responsible research and innovation measures. Methods An extensive review of the health research impact literature and of multistakeholder initiatives resulted in a database of 1556 impact indicators. The MSC was then cocreated by engaging key stakeholders and conducting semi-structured interviews of experts in the field. Results The MSC consists of five accountability dimensions: excellence, efficacy, economic, social and patient-reported outcomes. The tool contains 125 potential indicators, classified into 53 impact measurement aspects that are considered the most relevant topics for multistakeholder research and innovation initiatives when assessing their impact on the basis of their mission and their stakeholders’ interests. The scorecard allows the strategic management of multistakeholder research initiatives to demonstrate their impact on people and society. The value of the tool is that it is comprehensive, customizable and easy to use. Conclusions The MSC is an example of how the views of society can be taken into account when research impacts are assessed in a more sustainable and balanced way. The engagement of patients and other stakeholders is an integral part of the CRIF, facilitating collaborative decision-making in the design of policies and research agendas. In policy making, the collective approach allows the evaluation perspective to be extended to the needs of society and towards responsible research and innovation. Multidimensionality makes research and innovations more responsive to systemic challenges, and developing more equitable and sustainable health services.

Abstract The COVID-19 pandemic has unmasked even more clearly the need for research and care to form a unique and interdependent ecosystem, a concept which has emerged in recent years. In fact, to address urgent and unexpected missions such as “fighting all together the COVID-19 pandemic”, the importance of multi-stakeholder collaboration, mission-oriented governance and flexibility has been demonstrated with great efficacy. This calls for a policy integration strategy and implementation of responsible research and innovation principles in health, promoting an effective cooperation between science and society towards a shared mission. This article describes the MULTI-ACT framework and discusses how its innovative approach, encompassing governance criteria, patient engagement and multidisciplinary impact assessment, represents a holistic management model for structuring responsible research and innovation participatory governance in brain conditions research.

Abstract: Recently is growing the need for non-invasive, fast, and accurate technologies that can predict seed quality. Between these technologies, X-ray image analysis stand out for evaluation of the internal morphology of the seeds. Thus, the aim of the present study was to evaluate the efficiency of a specialized software for analyzing digital radiographs of Urochloa decumbens seeds called SARS (Sistema de Análise de Radiografias de Sementes - Seed Radiograph Analysis System). Five comercial seed lots of U. decumbens cv. Basilisk were used. The seed lots were produced in the 2018/2019 crop season. Radiographic images of the seeds were analyzed in SARS, through which physical characteristics were obtained. The seeds were then subjected to germination test, in which variables related to the physiological quality were evaluated. It was possible to observe that the seeds with greater germination and vigor showed strong and significant correlations with some of the physical variables obtained using SARS. Thus, high correlation of seedling length and relative seed density is important for validating the seed radiographic image analysis method. SARS proved to be an efficient tool for analyzing digital radiographs of U. decumbens seeds. It can generate descriptors which support morphometric and internal analysis of the seeds. Physical parameters obtained by using the technique have close relationship with the germination and vigor of the seeds.

Abstract: Atmospheric plasma application technology consists of a fast and practical method, which has the potential to modify the surface of seeds with integumentary hardness. The aim of this research was to evaluate the effect of applying atmospheric cold plasma on Pityrocarpa moniliformis seeds, assessing its implications on the seed coat impermeability, germination and vigor. Cells were subjected to plasma for 1.5, 2.0, 3.0, 4.0 and 5.0 minutes, using seeds without any treatment as a control. After application, the seed coat wettability, imbibition curve and electrical conductivity of the soaking solution were determined. Seeds were also subjected to the germination test, and its results were used to determine viability, median, uniformity and asymmetry of germination. Seeds subjected to plasma for 5.0 and 4.0 minutes showed the lowest apparent contact angles, 64 and 61°, respectively, characterizing greater wettability of the seed coat among the treatments tested. Higher germination was observed in the treatments with plasma application when compared to the control. Atmospheric plasma application technology has the potential to be used as an accelerator of P. moniliformis seed germination.

Prefácio

The economically profitable production of crops is related, among other factors, to seed quality, the production system, and the water used in irrigation or preparation of nutrient solutions. Therefore, the objective was to evaluate the phenology, production, and vigor of seeds of mini watermelons grown in saline nutrient solution and different substrates. In the fruit and seed production phase, the experiment occurred in a greenhouse with five electrical conductivities of water for nutrient solution preparation, ECw (0.5, 2.4, 4.0, 5.5, and 6.9 dS m−1), and two growing substrates (coconut fiber and sand). We evaluated the physiological quality of seeds previously produced under the five electrical conductivities of water and two substrates. High salinities for the hydroponic cultivation of the mini watermelon cultivar ‘Sugar Baby’ accelerated fruit maturation and crop cycle, decreasing fruit size. However, in both substrates, the seed production of mini watermelons, seed viability, and seed vigor occurred adequately with a reject brine of 6.9 dS m−1 in the hydroponic nutrient solution. The seed production of ‘Sugar Baby’ mini watermelons using reject brine in a hydroponic system with coconut fiber and sand substrates is viable in regions with water limitations.

Stress caused by defoliation can compromise the physiological quality of soybean seeds. However, there is evidence that the application of nitrogen (N) can be an alternative to reduce the qualitative losses in seeds caused by defoliating insects. The objective of this study was to evaluate the effectiveness of topdressing N fertilization in improving the physiological quality of soybean seeds from plants subjected to artificial defoliation. The experimental design was a randomized block arranged in a 2x4 factorial scheme with four replicates. The treatments consisted of two levels of defoliation (33 and 66%) and four doses of N (0, 50, 100, and 150 kg ha-1), using urea (45% N), applied two days after defoliation. Artificial defoliation was performed during the R3 stage by removing one (33%) and two leaflets (66%) from each trifoliate leaf under field conditions. At stage R5.1, leaf area was quantified, and after harvesting, germination, emergence, length, total dry mass, electrical conductivity, total protein content, mass of 1000 seeds, and tetrazolium (vigor and viability) tests were carried out. It was concluded that increasing the defoliation from 33 to 66% impaired the physiological quality of soybean seeds. The use of nitrogen fertilization for coverage after defoliation at the beginning of the reproductive phase cannot minimize qualitative losses in seeds. [ABSTRACT FROM AUTHOR]

Harvest delay can alter the physiological and sanitary quality of sorghum seeds due to the longer exposure time of seeds to adverse environmental conditions in the field. In this study, we investigated the impact of delayed Sorghum sp. harvest on the physiological and sanitary quality of seeds in four hybrids grown in the tropical soil of the Brazilian Cerrado region. Plants from four Sorghum sp. hybrids (IPA SUDAN 4202, BRS 658, BRS 810, and BRS 373) were grown and submitted to four seed harvest periods (0, 7, 14, and 21 days after stage 9 -- seed physiological maturity). The treatments were arranged in a completely randomized block design in a 4 x 4 factorial, with four replicates. At each harvest period, the t housand-seed weight, water content, first count of the germination test, germination, seedling emergence, emergence speed index, seedling dry matter, accelerated aging, electrical conductivity, and sanitary quality of seeds were determined. Seeds of higher physiological and sanitary quality of the IPA SUDAN 4202 and BRS 658 sorghum hybrids can be obtained when the harvest is carried out up to 7 days after the physiological maturity stage. However, the delay in seed harvesting at 14 and 21 days after physiological maturity results in a higher incidence of Colletotrichum sp., Curvularia sp., Rhizoctonia sp., and Alternaria sp. on the seeds of these hybrids. Delaying the harvest of sorghum seeds from 7 days after the physiological maturity stage compromises seed vigor and viability and increases the incidence of pathogens. [ABSTRACT FROM AUTHOR]

This study investigated the effectiveness of using the X-ray technique to assess the viability of papaya seeds. The X-ray images were performed in eight replicates of 25 seeds, placed on transparent acrylic plates on double-sided adhesive tape, previously identified, and then submitted to radiation in X-ray equipment "HP Faxitron". After exposure to X-ray radiation, the seeds were submitted to the germination test in rolls of germitest paper and kept in a germination chamber at 20-30 °C for 30 days. Seedlings were photographed and evaluated as normal seedlings, abnormal and non-germinated seeds. Afterward, the X-ray images and the seedlings from the same seeds were visually compared, and the X-ray images clearly showed seeds with and without internal filling. All abnormal seedlings were full seeds; therefore, X-ray images were not able to separate normal and abnormal. A 100% accuracy was verified by comparing the X-ray images and their respective seedlings, full seeds originated normal or abnormal seedlings, and empty seeds corresponded to non-germinated seeds. Therefore, using X-ray images is a promising technique to identify the viability of papaya seeds. The automated analysis of X-ray images is a simple, fast, and efficient technique to provide information on seed viability and generate parameters related to their germination capacity. [ABSTRACT FROM AUTHOR]

The root protrusion test is a vigor test based on the principle that seeds with high vigor emit the primary root faster than less vigorous ones. Chia is a plant propagated by seeds and it is studied owing to its health benefits, but there is little technical information known about its culture. The objective of this work was to adapt the methodology of the root protrusion test to determine the vigor of chia seed lots. Five chia seed lots were submitted to an initial quality evaluation using germination and vigor tests (first germination, emergence, and emergence speed index). The root protrusion test was performed at temperatures of 20, 25 and 30 °C and evaluated every 2 h up to 42 h. During the test, protrusion stability was assessed and the criterion of 2 mm primary root count was used. The root protrusion test at 20 °C did not distinguish the lots in the same way as emergence. At 25 °C it was possible to classify lots after 30 h similarly to emergence. At 30 °C, root protrusion and the separation of seed lots was accelerated. Thus, the root protrusion test conducted for 30 h at 25 °C has sufficient sensitivity to detect differences in vigor between chia seed lots.

The gold standard for the management of sleep bruxism (SB) is the use of a rigid occlusal splint; however, there are limitations for its use in children and alternatives to the management of SB are needed. Photobiomodulation therapy has been used with positive results in temporomandibular disorders. This study aimed to evaluate the effects of photobiomodulation therapy with infrared LED in children with SB. Thirty children were divided into three groups: Group 1: control/absence of bruxism (n = 10); Group 2: SB treated with infrared LED (n = 10); Group 3: SB treated with occlusal splint (n = 10). Electromyographic evaluation of masseter, anterior temporalis, and upper trapezius, and salivary dopamine levels were assessed before and after treatments. Data were statistically analyzed using two-way mixed model ANOVA. An increase in the temporalis and right masseter EMG activity at rest was observed in Group 3, with large effect size (p < 0.05). Left masseter and temporalis EMG activity did not differ over time in the LED group, similar to the control group. Moreover, the EMG activity of masticatory muscles during chewing and upper trapezius muscle did not differ over time in all groups. The results also pointed to a difference in the levels of dopamine between children with and without SB, with Group 3 showing higher levels in the pre-treatment time compared to controls (p < 0.025). In conclusion, an increase in the masticatory muscles activity at rest was observed in children undergoing splint therapy. Moreover, a difference in the levels of salivary dopamine was found between children with and without SB.

The safety of the transport of dangerous goods can highly benefit from an increased awareness of this activity. In particular, quantitative information about the amount of substances transported and their features can support further research and operative activities. This paper describes the approach of monitoring activities adopted by the Motorway Concessionaire Autovie Venete S.p.A. on the A4 highways Venezia-Trieste, in the northeast of Italy. Firstly, the paper briefly describes the architecture of the system, currently based on 24 on-line DGT monitoring gantries, and its functionalities. Some samples of the possible extractions of the collected data are presented. Then, an overview about how the Concessionaire uses the data collected are is provided. Besides the relevant aim of data collection for a better knowledge about DGT on the highway, the system implemented is intended to support the Road Operator in its strategy to increase safety. Data allowed, and the process is still ongoing, the fine-tuning of procedures, measures and actions for the effective management of DGT. Final consideration about the possible future scenarios is provided.

Chia (Salvia hispanica) is an annual oilseed crop of high nutritional value. This study aimed to analyze the performance of chia plants sown at different times and grown under different phosphorus rates. The experiment was conducted under field conditions at the Experimental Farm of the State University of Maringá, Umuarama, Brazil. Treatments were arranged in a randomized split-plot design with three replications. Phosphorus fertilizer was applied at the time of sowing at rates of 0, 40, 80, or 120 kg P2O5 ha−1. Seeds were sown on four dates at 14-day intervals, starting on March 21, 2017, for the first crop and March 29, 2018, for the second crop. Plant population density, raceme number, shoot dry matter yield, thousand seed weight, seed yield, and seed oil content were determined. It was observed that the end of March is the borderline for chia cultivation, because the low temperature and rainfall conditions occurring after this period compromise chia growth. The best phosphorous rate for chia growth was 80–120 kg ha−1, which led to optimum vegetative parameters.

Rice seeds coating with microparticulate zinc oxide can maximize the absorption of this micronutrient by the seedling and favor seeds vigor when compared with zinc sulfate. This work aimed to evaluate the sources and doses of zinc in seed quality and in the nutrition of rice seedlings. The experiment consisted of a completely randomized design with four replications, five doses of zinc in the form of oxide (0; 20; 40, 80 and 160 g kg-1), and a control treatment of zinc in the form of sulfate (40 g kg-1). The following parameters were evaluated: germination, accelerated aging, seedlings emergence, emergence speed index, cold test, dry matter, and zinc concentration in the root and shoot of the seedlings. Seed coating with zinc, regardless of the source, improved seed physiological quality and seedling nutrition of the rice cultivar. Zinc sources did not change the germination; however, the oxide form provided better seed vigor, except for the highest dose (160 g kg-1). The increase in the dose of zinc oxide increased the concentration of this nutrient in rice seedlings dry matter. Zinc coating of rice seeds in the form of microparticulate, depending on the dose, can maximize the seeds vigor and increase the concentration of this micronutrient in the seedlings when compared with zinc sulfate, which is the traditional source used by producers. Highlights Zinc (Zn) coating does not affect germination, but influences the vigor of rice seeds. The Zn sources affect the Zn concentration in the shoot dry matter. Both ZnO and ZnSO 4 showed higher shoot dry matter, when compared with the absence of Zn in rice seeds. Oxide zinc (ZnO) promotes greater root dry matter of rice when compared with zinc sulfate (ZnSO 4 ).

Different regions have different environmental conditions, which may be unfavorable for the preservation of the quality of stored soybean seeds over time. Thus, it is necessary to adopt specific technologies to control the storage environment conditions. Big raffia bags are widely used for the storage of soybean seeds, however these consist of a porous, permeable material that allows the exchange of gases between the packaging and the storage environment. In an effort to find a solution to this problem, in this study we evaluated low cost big bag coating alternatives, in order to minimize the effects of temperature and intergranular humidity on stored seeds. Thus, the aim of this work was to evaluate the quality of soybean cultivars subjected to different temperature and storage duration conditions and stored in raffia bags with or without internal coating. We used a completely randomized, three-factor (10 × 6 × 5) experimental design. We assessed 10 soybean cultivars, six storage environments, and five evaluation periods. Our results showed that seeds of the M-SOY 8866, M7110 IPRO, CD 2737 RR, and BMX DESAFIO 8473 RSF soybean cultivars preserved their physiological quality better in different storage environments. The storage duration had a cumulative effect on the negative factors that favor the deterioration of the quality of the stored seeds. The storage temperature was the main factor that affected the physiological quality of the stored seeds. The use of coated packaging was beneficial in preserving the physiological quality of stored soybean seeds; however, its effect was greater at ambient temperature than in a cold environment. The best storage environment for the preservation of the quality of the seeds was characterized by 10°C temperature conditions and the use of coated packaging, while the worst storage environment was characterized by ambient temperature conditions without the use of coated packaging. Thus, it was concluded that the use of coatings in raffia big bags can be an alternative for maintaining the quality of seeds of different soybean cultivars during storage in seed processing units.

As sementes de graviola (Annona muricata) apresentam tegumento resistente e impermeável, além de substâncias inibidoras que dificultam a germinação das sementes. Assim, objetivou-se avaliar a contribuição da embebição em ácido giberélico para a superação da dormência e aumento da germinação das sementes de graviola. O delineamento experimental foi inteiramente casualizado, com cinco tratamentos e quatro repetições. As sementes foram escarificadas e em seguida embebidas por 24 horas a 25 ºC em soluções contendo 0; 50; 100; 150 e 200 mg L-1 (ppm) de ácido giberélico. Após este período de embebição as sementes foram submetidas ao teste de germinação, sendo avaliados a porcentagem de germinação, o índice de velocidade e o tempo médio de germinação. As concentrações de ácido giberélico apresentaram efeito sobre a germinação e índice de velocidade de germinação. Conclui-se que a embebição das sementes de graviola na concentração aproximada de 140 ppm de ácido giberélico auxilia na superação da dormência, aumentando a germinação e o índice de velocidade de germinação.

This study aimed to evaluate the effects of chemical treatment of seeds with fungicide and insecticide on the seed physiological quality of Brachiaria brizantha cultivar MG5. Two experiments with four replicates were carried out in a completely randomized design. In the first experiment, the seeds were treated with the fungicide pyraclostrobin + fipronil + thiophanate-methyl and in the second, with the insecticide thiamethoxam, both at doses of 0, 150, 300, 450, and 600 mL of commercial product (CP)/100 kg of seeds. Physiological characterization was done on the basis of the first germination count, germination (%), emergence (%), emergence speed index, and length and dry mass of shoot and root. The treatment of B. brizantha seeds with the fungicide pyraclostrobin + fipronil + thiophanate-methyl benefits the physiological quality of seeds, improving germination and root development, with no phytotoxic effect up to the dose of 600 mL of CP/100 kg of seeds. The insecticide thiamethoxam has a biostimulating effect on B. brizantha cultivar MG5 up to the dose of 270 mL of CP/100 kg of seeds, but is phytotoxic in larger doses.

The present study aimed to assess the variations in the parameters of the exudate pH and flooding tests to evaluate the physiological quality of soybean seeds. We subjected the lots of soybean seeds to germination, emergence, exudate pH, and flooding tests. Further, water content of the seeds, first count of germination, and emergence speed index were also determined. The exudate pH test studied the variations in the soaking period and temperature, while the flooding test assessed the variations in the amount of water and temperature at different periods of immersion. The experiment design was completely randomized. The results were presented as means and were compared using the Scott-Knott test at 5% probability. The Pearson’s correlation coefficient between the exudate pH and emergence of seedlings, and also between the flooding test result and emergence of seedlings were calculated. The results of the study indicated that the exudate pH test can be used to evaluate the physiological quality of soybean seeds after 30 min of imbibition at 20 °C. Further, the flooding test was efficient in evaluating the vigor of soybean seeds after 4 h of immersion either in 50 mL of distilled water at 25 °C or in 75 mL of distilled water at 30 °C.

The development of technologies to increase stand establishment efficiency and seed vigor, as well as to maximize profits, is of fundamental importance for the sustainability of agriculture. Products based on humic substances, such as humic acids, have been recently used in Brazilian agriculture to produce seedlings with high vigor, which positively influences the establishment of the initial stand. Thus, this study aimed to evaluate the effect of treatment of corn seeds with a commercial humic acid-based product, Humykos® (18% of humic acid content), on germination and seedling vigor. The treatments consisted of six doses (0, 100, 200, 300, 400, and 500 mL 100 kg-1 seed) of Humykos®, and the treated seeds were analyzed for germination and vigor (first count, emergence, emergence speed index, length, and dry mass of shoots and roots). Our results show that humic acid promotes greater seedling growth and increased shoot dry mass in corn; in addition, it has a positive influence on the emergence speed index up to a dose 158 mL 100 kg-1 seeds.

RESUMO A importância econômica do mogno ressalta a relevância da análise do vigor de suas sementes visando à formação de mudas com qualidade. O objetivo foi avaliar a influência de diferentes temperaturas e períodos de exposição do teste de envelhecimento acelerado em sementes de mogno. As variáveis analisadas foram: teor de água, emergência de plântulas, índice de velocidade de emergência, freqüência relativa e tempo médio de emergência após o envelhecimento acelerado [(39; 41; 43 e 45 ?C, durante zero (testemunha), 24; 48; 72 e 96 horas]. O vigor de sementes de mogno pode ser avaliado por meio do teste de envelhecimento acelerado nas temperaturas de 39 ºC/96 horas e 43 ºC por 48 ou 96 horas. A combinação 43 ºC/48 horas permite reduzir o tempo de execução das avaliações de sementes de mogno. O índice de velocidade de emergência é uma variável adequada para avaliar a influência do envelhecimento acelerado sobre sementes de mogno.

Chia (Salvia hispanica L.) is a plant native to central and western Mexico and northern Guatemala belonging to the Lamiaceae family, well known for its seeds containing antioxidants, dietary fibers, and ?-linolenic acid, which helps to prevent different diseases, such as diabetes, obesity, cancer and Alzheimer. In Brazil, there is not much information about the crop, mainly about the physiological quality of its seeds. Therefore, the objective was to establish vigor testing methods to evaluate the physiological quality of chia seeds. Five seed lots were used, in which germination tests, seedling emergence, emergence velocity index and variations in the electrical conductivity test were carried out. The volume of distilled water was 25 and 50 mL, the number of seeds was 50 and 100, the temperature was 20, 25 and 30 °C, the soaking period was 1, 2, 4, 6, 12, 18 and 24 hours and variations in there was accelerated aging test by the traditional method and saturated NaCl solution, being periods of aging as 24, 48, 72 and 96 hours and temperatures of 38, 41 and 44 °C. It is concluded that both the electrical conductivity test and the accelerated aging test are efficient in evaluating the physiological quality of chia seeds. The electrical conductivity test must be performed with 100 seeds in 50 mL of distilled water, for four hours soaking at 25 or 30 °C; and the accelerated aging test should be performed at 44 °C for 24 hours by the traditional method or 41 °C for 24 hours using saturated NaCl solution.

This study describes the use of X-ray fluorescence spectroscopy in Crotalaria ochroleuca seed technology. This work evaluated X-ray fluorescence techniques to estimate the physiological performance of different C. ochroleuca seed coat colours based on the concentration and distribution of Ca, P, K, and S in seed structures. The treatments consisted of seeds separated by coat colours (yellow, green, and red) and a control treatment (colour mix according to their natural occurrence in commercial lots), and was carried out in a completely randomized design, with four replications. The physiological performance was evaluated by analyzing the water content, germination, first germination count, germination speed index, electrical conductivity, seedling emergence, and seedling length and dry mass. X-ray fluorescence spectroscopy techniques were carried out with quantitative analyses (Ca, P, K, and S concentration in the seed coat and the whole seed) and qualitative analyses (macronutrient mapping). The EDXRF and μ-XRF techniques are efficient and promising to differentiate the physiological performance of C. ochroleuca seeds, based on the concentration and distribution of Ca, P, K, and S in different structures. Ca is predominant in the seed coat, and K, S, and P are found throughout the embryonic axis. Seeds of yellow and green coats have higher nutrients concentration and distribution in the embryonic axis, revealing high germinative capacity and physiological performance. Seeds of red coat have higher nutrients concentration in the seed coat and lower assimilation, showing less vigour, which interferes directly in the quality of commercial lots.

The accelerated aging test has stood out among vigor tests. It has been used with different objectives, including as an assistance method in the selection of cultivars for plant breeding. This research aimed to analyze the effects of different exposure times to accelerated aging on the germination and vigor of sorghum seeds from different genetic sources. The experiment consisted of a completely randomized design with eight genotypes and four replications. Seeds were subject to an accelerated aging test with four exposure times (0, 24, 72, and 96 h) at a temperature of 47 °C and then evaluated by germination test (G), germination speed index (GSI), mean germination time (MGT), seedling emergence (SE), emergence speed index (ESI), and mean emergence time (MET). All genotypes presented a reduction in germination, GSI, SE, and ESI, and an increase in MGT and MET. The genotype CMX5156A had an increase in seed vigor under conditions of high temperature and relative humidity. Accelerated aging for 72 hours at 47 °C drastically reduces seed vigor. The accelerated aging test can be used to differentiate sorghum genotypes in breeding programs.

Physiological and sanitary seed quality is essential for rapid and uniform crop establishment at field, a factor which contributes to vegetable crop production success. The aim was to evaluate physiological and sanitary quality of lettuce and cabbage seeds coming three lots acquired in trade. Physiological quality was determined by means of germination test and index of speed germination. Health status was assessed through sanity test in Petri dishes containing BDA medium. The experimental design was completely randomized with four replications and the averages of each lot compared among themselves by Tukey test (5%). No statistical analyzes were performed to health test and samples were evaluated for presence of microorganisms on the plates. Aspergillus spp., Rhizopus spp., cocci and bacillus are associated with lettuce seeds, and Aspergillus spp., cocci and bacillus are associated with cabbage seeds, but this association can not interfere with germination performance at laboratory. Information about germination contained in the package do not always coincide with those examined in situ. Lettuce and cabbage seeds are being marketed carrying pathogens.

ABSTRACT: Crambe is a rapeseed with high oil content and can be used as a winter cover or as a source of raw material for the production of biodiesel, however espite the growing interest in the culture, research on the subject is still incipient, especially concerning the seed production and analysis technology. The purpose of this study is to evaluate the physiological quality of crambe seeds, 'FMS Brilhante' cultivar, by testing the pH of exudate. Five seed lots were submitted to the determination of water content and the tests of germination and vigor (first count, emergence and tetrazolium). In the conduction of pH exudate test, temperatures (25 and 30oC), and periods of seed imbibition in water (15, 30 and 45 minutes) were tested. The experiment was conducted in a completely randomized manner, with four replicates, and the mean values were compared by the Tukey test at 5% probability; Pearson correlation between the pH of the exudate and initial tests was also made. Testing the pH of exudate is promising for separating lots of crambe seeds and the following combinations of 25°C/30 minutes or 30°C/45 minutes can be used.

The Cerrado occupies 25% of the Brazilian territory and is the second largest biome in South America, second only to the Amazon Rainforest. Hence, studies on the native species of the Cerrado are important for implementing conservation strategies. In this context, knowledge of the requirements and conditions for germination of seeds of these native species is essential. The Cerrado watermelon ( Melothria campestris (Naudin) H. Schaef. & S.S. Renner) is a plant that is not cultivated, but has been used in revegetation projects in the countryside of the Cerrado because its fruits are fed upon by fauna in times of food shortage. The purpose of this study was to determine the most appropriate methodology for the germination of the Cerrado watermelon, by identifying the best - suited substrate, the optimal temperature, and the corresponding time taken for germination . The treatments consisted of four substrates: in a paper roll, on paper, in sand, and on sand; and four temperatures: 20, 25, and 30 °C (maintained constant), and a fourth temperature treatment that alternated 20 - 30 °C. The effect of the substrates and temperatures on seed performance was evaluated using the germination test, speed index, and average time for germination. The experimental design was fully randomized, with treatments distributed in a 4 × 4 (substrates × temperatures) factorial scheme, with four replications. Our results show that the temperature of 20 °C had a negative influence on germination in all substrates. Further, we found that the germination of the Cerrado watermelon seeds was best in a paper roll or on sand substrate, maintained at a constant temperature of 30 °C, or when temperature was alternated 20 - 30 °C, and the time required for germination stabilization was 30 days.

The environmental and economic potential of long pepper (Piper hispidinervum) requires the development of methodology to evaluate seed potential physiological. The work aimed to evaluate the physiological potential of long pepper seeds (Piper hispidinervum C. DC.) through different tests and accelerated aging. Seeds were harvested in four different commercial planting and evaluated to water content (%), germination (G%), germination speed index (GSI), seedling emergence (SE%), emergence rate index (ERI) and accelerated aging (AA 41 and 45 °C during 24, 48, 72 and 96 hours). The experimental design was completely randomized with a simple scheme for G, GSI, SE and ERI, and 4 x 4 factorial (lots and times) at each temperature for AA. Physiological potential of long pepper seeds can be evaluated by germination test, germination speed index, seedling emergence, seedlings emergence speed index and accelerated aging at 41 °C during 24 hours.