1. THE SHORTENED ISOFORM OF a-TUBULIN IS DETECTED IN COMPLEX WITH PROTEASOMES.
- Author
-
Ivanova EY, Artamonova TO, Zaykova YY, Khodorkovskii MA, and Tsimokha AS
- Subjects
- HEK293 Cells, Humans, K562 Cells, Protein Isoforms chemistry, Protein Isoforms isolation & purification, Protein Isoforms metabolism, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Proteasome Endopeptidase Complex chemistry, Proteasome Endopeptidase Complex isolation & purification, Proteasome Endopeptidase Complex metabolism, Tubulin chemistry, Tubulin isolation & purification, Tubulin metabolism
- Abstract
The proteasome is a multi-subunit protein complex that serves as a major pathway for intracellular protein degradation playing important functions in various biological processes. By using MALDI-ICR-mass-spectrometry and Western-blot analysis, we have shown the presence of shortened isoform of a-tubulin in complex with the affinity-purified proteasomes from stable cell lines K562 and HEK293.
- Published
- 2017