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AIM: The present paper investigated the effect on behavior of female rat 3 months after electromagnetic pulse irradiation. MATERIALS AND METHODS: Mature female rats were randomly divided into control group and EMP irradiation group. Rats in the EMP irradiation group were exposed to EMP. The field intensity was 100 KV/m with 1000 pulses. The changes of behavior were observed with step through test and open-field test in rats 3 months after electromagnetic pulse irradiation. Memory retention, Error scores, Accumulative Distance (AD), Accumulative Distance of Leaving the Border (ADLB), Accumulative Distance in the User Defined Area (ADUDA), AD inside the inner circle(ADIC), AT of Resting (ATREST), AT of Walking (ATWALK) and AD of Walking(ADWALK) were recorded. RESULTS: Analysis of step through test indicated that the EMP irradiation group has a significant decrease in memory retention and a significant increase in error scores of the entries into the dark compartment compared with control group (P