Search

Your search keyword '"Zeng, Weian"' showing total 189 results
Start Over Author "Zeng, Weian"
189 results on '"Zeng, Weian"'

4. METTL14-mediated m6A epitranscriptomic modification contributes to chemotherapy-induced neuropathic pain by stabilizing GluN2A expression via IGF2BP2

Author

Lu, Weicheng, Yang, Xiaohua, Zhong, Weiqiang, Chen, Guojun, Guo, Xinqi, Ye, Qingqing, Xu, Yixin, Qi, Zhenhua, Ye, Yaqi, Zhang, Jingyun, Wang, Yuge, Wang, Xintong, Wang, Shu, Zhao, Qiyue, Zeng, Weian, Huang, Junting, Ma, Huijie, and Xie, Jingdun

Subjects
Thermo Fisher Scientific Inc., Methyl aspartate, Genes, Aspartate, Chemotherapy, Pain -- Care and treatment, Cancer -- Chemotherapy, Neurons, Scientific equipment and supplies industry, Gene expression, Methylation, Methyltransferases, Epigenetic inheritance, Neurophysiology, Health care industry
Abstract

Epigenetics is a biological process that modifies and regulates gene expression, affects neuronal function, and contributes to pain. However, the mechanism by which epigenetics facilitates and maintains chronic pain is poorly understood. We aimed to determine whether [N.sup.6]-methyladenosine (m6A) specifically modified by methyltransferase-like 14 (METTL14) alters neuronal activity and governs pain by sensitizing the GluN2A subunit of the N-methyl-D-aspartate receptor (NMDAR) in the dorsal root ganglion (DRG) neurons in a model of chemotherapy-induced neuropathic pain (CINP). Using dot blotting, immunofluorescence, gain/loss-of-function, and behavioral assays, we found that m6A levels were upregulated in L4-L6 DRG neurons in CINP in a DBP/METTL14-dependent manner, which was also confirmed in human DRGs. Blocking METTL14 reduced m6A methylation and attenuated pain hypersensitivity. Mechanistically, METTL14-mediated m6A modification facilitated the synaptic plasticity of DRG neurons by enhancing the GluN2A subunit of NMDAR, and inhibiting METTL14 blocked this effect. In contrast, overexpression of METTL14 upregulated m6A modifications, enhanced presynaptic NMDAR activity in DRG neurons, and facilitated pain sensation. Our findings reveal a previously unrecognized mechanism of METTL14-mediated m6A modification in DRG neurons to maintain neuropathic pain. Targeting these molecules may provide a new strategy for pain treatment., Introduction Chemotherapy-induced neuropathic pain (CINP) is a debilitating complication caused by damage to the peripheral nervous system by chemotherapeutic agents, including paclitaxel (1). CINP occurs in approximately 50% to 90% [...]

Published
2024
Full Text
View/download PDF

10. Sex differences in PD‐L1‐induced analgesia in paclitaxel‐induced peripheral neuropathy mice depend on TRPV1‐based inhibition of CGRP.

Author

Cao, Yan, Jiang, Wenqi, Yan, Fang, Pan, Yuyan, Gei, Liba, Lu, Simin, Chen, Xiangnan, Huang, Yang, Yan, Yan, Feng, Yan, Li, Qiang, Zeng, Weian, Xing, Wei, and Chen, Dongtai

Abstract

Aims: Paclitaxel (PTX) is extensively utilized in the management of diverse solid tumors, frequently resulting in paclitaxel‐induced peripheral neuropathy (PIPN). The present study aimed to investigate sex differences in the behavioral manifestations and underlying pathogenesis of PIPN and search for clinically efficacious interventions. Methods: Male and female C57BL/6 mice (5–6 weeks and 12 months, weighing 18–30 g) were intraperitoneally (i.p.) administered paclitaxel diluted in saline (NaCl 0.9%) at a dose of 2 mg/kg every other day for a total of 4 injections. Von Frey and hot plate tests were performed before and after administration to confirm the successful establishment of the PIPN model and also to evaluate the pain of PIPN and the analgesic effect of PD‐L1. On day 14 after PTX administration, PD‐L1 protein (10 ng/pc) was injected into the PIPN via the intrathecal (i.t.) route. To knock down TRPV1 in the spinal cord, adeno‐associated virus 9 (AAV9)‐Trpv1‐RNAi (5 μL, 1 × 1013 vg/mL) was slowly injected via the i.t. route. Four weeks after AAV9 delivery, the downregulation of TRPV1 expression was verified by immunofluorescence staining and Western blotting. The levels of PD‐L1, TRPV1 and CGRP were measured via Western blotting, RT–PCR, and immunofluorescence staining. The levels of TNF‐α and IL‐1β were measured via RT–PCR. Results: TRPV1 and CGRP protein and mRNA levels were higher in the spinal cords of control female mice than in those of control male mice. PTX‐induced nociceptive behaviors in female PIPN mice were greater than those in male PIPN mice, as indicated by increased expression of TRPV1 and CGRP. The analgesic effects of PD‐L1 on mechanical hyperalgesia and thermal sensitivity were significantly greater in female mice than in male mice, with calculated relative therapeutic levels increasing by approximately 2.717‐fold and 2.303‐fold, respectively. PD‐L1 and CGRP were partly co‐localized with TRPV1 in the dorsal horn of the mouse spinal cord. The analgesic effect of PD‐L1 in PIPN mice was observed to be mediated through the downregulation of TRPV1 and CGRP expression following AAV9‐mediated spinal cord specific decreased TRPV1 expression. Conclusions: PTX‐induced nociceptive behaviors and the analgesic effect of PD‐L1 in PIPN mice were sexually dimorphic, highlighting the significance of incorporating sex as a crucial biological factor in forthcoming mechanistic studies of PIPN and providing insights for potential sex‐specific therapeutic approaches. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

14. Amiloride alleviates morphine tolerance by suppressing ASIC3-dependent neuroinflammation in the spinal cord

Author

Gei, Liba, primary, Yan, Yan, additional, Xing, Wei, additional, Li, Qiang, additional, Chen, Xiangnan, additional, Yan, Fang, additional, Wang, Yan, additional, Cao, Yan, additional, Jiang, Wenqi, additional, RiQi, E., additional, Luo, DeXing, additional, Zhang, YanHong, additional, Zeng, Weian, additional, and Chen, Dongtai, additional

Published
2023
Full Text
View/download PDF

29. Mutation-Derived Long Noncoding RNA Signature Predicts Survival in Lung Adenocarcinoma

Author

Yang, Longjun, primary, Guo, Guangran, additional, Yu, Xiangyang, additional, Wen, Yingsheng, additional, Lin, Yongbin, additional, Zhang, Rusi, additional, Zhao, Dechang, additional, Huang, Zirui, additional, Wang, Gongming, additional, Yan, Yan, additional, Zhang, Xuewen, additional, Chen, Dongtai, additional, Xing, Wei, additional, Wang, Weidong, additional, Zeng, Weian, additional, and Zhang, Lanjun, additional

Published
2022
Full Text
View/download PDF

33. Additional file 3 of Ganglioside-monosialic acid (GM1) for prevention of chemotherapy-induced peripheral neuropathy: a meta-analysis with trial sequential analysis

Author

Wu, Shaoyong, Bai, Xiaohui, Guo, Caixia, Huang, Zhimei, Ouyang, Handong, Huang, Jingxiu, and Zeng, Weian

Abstract

Additional file 3: Supplementary Fig. S1. Sensitivity analysis of forest plot displaying a random-effects meta-analysis of the effect of GM1 on the incidence of CTCAE grade ��� 2 when only RCTs were included.

Published
2021
Full Text
View/download PDF

38. Intravenous versus Volatile Anesthetic Effects on Postoperative Cognition in Elderly Patients Undergoing Laparoscopic Abdominal Surgery

Author

Li, Yujuan, primary, Chen, Dongtai, additional, Wang, Hanbing, additional, Wang, Zhi, additional, Song, Furong, additional, Li, Hui, additional, Ling, Li, additional, Shen, Zhiwen, additional, Hu, Chuwen, additional, Peng, Jun, additional, Li, Weixing, additional, Xing, Wei, additional, Pan, Jiahao, additional, Liang, Hua, additional, Zhou, Qiaoling, additional, Cai, Jun, additional, He, Ziqing, additional, Peng, Shuling, additional, Zeng, Weian, additional, and Zuo, Zhiyi, additional

Published
2021
Full Text
View/download PDF

40. Impact of anesthesia methods on perioperative systemic inflammation and long-term outcomes in patients undergoing surgery for hepatocellular carcinoma: a propensity score-matched analysis

Author

Zhang, Hao, primary, Guo, Kefang, additional, Sun, Xingfeng, additional, Liu, Yi, additional, Zhong, Ziwen, additional, Zhou, Wenchang, additional, Zeng, Weian, additional, Wang, Lu, additional, Cata, Juan P., additional, Chen, Wankun, additional, and Miao, Changhong, additional

Published
2021
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results