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Your search keyword '"Zhang, Chang-Yin"' showing total 7 results
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7 results on '"Zhang, Chang-Yin"'

5. Nest RT-PCR to detect the West Nile virus.

Author

Song Jie, Tang Tai-shan, Tian Ling-ling, Zhang Chang-yin, Lei Zhi-hai, Sun Xu-hui, Chen Guo-qiang, Zhao Jian, Wang Kai-min, and Jiang Yan

Abstract

The article discusses a study on the development of two nest-polymerase chain reaction (PCR) for West Nile virus detection.

Published
2008

6. [Changes of large conductance Ca(2+)-activated K(+) channels on coronary smooth muscle cells from diabetic rats].

Author

Wang RX, Li XR, Yang ZY, Zheng J, Li KL, Zhang CY, Guo SX, Sun LP, and Lu T

Subjects
Animals, Calcium metabolism, Coronary Vessels metabolism, Male, Patch-Clamp Techniques, Rats, Rats, Sprague-Dawley, Diabetes Mellitus, Experimental metabolism, Large-Conductance Calcium-Activated Potassium Channels metabolism, Muscle, Smooth, Vascular metabolism
Abstract

Objective: To investigate the changes of large conductance Ca(2+)-activated K(+) channel (BK channel) on coronary smooth muscle cells from diabetic rats., Methods: Streptozotocin-induced rat diabetic animal model was used. Coronary smooth muscle cells were isolated by enzyme digestion. BK currents in control and diabetic groups were recorded by patch clamp technique in whole cell configuration, and BK channel protein expression was detected by Western blot. Calcium concentration was measured by fluorescence assay., Results: Compared with control group, BK current densities in diabetic group were significantly decreased when test potentials > 100 mV (P < 0.05). BK current densities were (275 ± 40) pA/pF in control group (n = 8) and (70 ± 10) pA/pF in diabetic group (n = 6) at 150 mV test potentials. α-subunit protein expression was similar between the groups (P > 0.05), however, β1-subunit protein expression was significantly reduced in diabetic group than in control group (P < 0.05). Calcium concentrations were significantly increased in diabetic group control group (151 ± 18) nmol/L (n = 6) than in control group (92 ± 7) nmol/L (n = 5, P < 0.05)., Conclusion: Observed β1-subunit downregulation, BK current density decrease and cytosolic calcium concentration increase in smooth muscle cells of diabetic coronary arteries may be associated with coronary dysfunction in diabetic rats.

Published
2010

7. [The pathological observation of acute intoxication by Alangium Chinese in mice].

Author

Zhang CY, Zhang LJ, Hu YL, Xia XD, Lu LM, Yan YF, Xu X, Ke W, and Zhu JH

Subjects
Acute Disease, Animals, Brain drug effects, Brain pathology, Disease Models, Animal, Dose-Response Relationship, Drug, Female, Forensic Pathology, Hemorrhage etiology, Hemorrhage pathology, Hepatocytes drug effects, Kidney drug effects, Kidney pathology, Lethal Dose 50, Liver drug effects, Lung drug effects, Male, Mice, Muscle, Smooth, Vascular drug effects, Plant Extracts administration & dosage, Plant Roots chemistry, Random Allocation, Toxicity Tests, Acute, Alangiaceae chemistry, Liver pathology, Lung pathology, Plant Extracts toxicity
Abstract

Objective: To investigate the pathological change of mice organ intoxicated by Alangium Chinese and its poisoning mechanism., Methods: Mice were intoxicated by gavage with extract of Alangium Chinese. Then the histopathologic examination was made for evaluating the pathological changes in the organs of the poisoned mice by HE staining., Results: The main pathological changes included alveolar hemorrhage, pulmonary interstitial hemorrhage, sinus hepaticus expansion and congestion, hepatocyte edema, subarachnoid hemorrhage, congestion and hemorrhage of other organs., Conclusion: The main target organs or tissue of Alangium Chinese are the lungs, liver and vascular smooth muscle. There is correlation between the toxic effect and the dosage.

Published
2009

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