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Bioorthogonal cycloaddition reactions between tetrazines and strained dienophiles are widely used for protein, lipid and glycan labelling because of their extremely rapid kinetics. However, controlling this chemistry in the presence of living mammalian cells with a high degree of spatial and temporal precision remains a challenge. Here we demonstrate a versatile approach to light-activated formation of tetrazines from photocaged dihydrotetrazines. Photouncaging, followed by spontaneous transformation to reactive tetrazine, enables live-cell spatiotemporal control of rapid bioorthogonal cycloaddition with dienophiles such as trans-cyclooctenes. Photocaged dihydrotetrazines are stable in conditions that normally degrade tetrazines, enabling efficient early-stage incorporation of bioorthogonal handles into biomolecules such as peptides. Photocaged dihydrotetrazines allow the use of non-toxic light to trigger tetrazine ligations on living mammalian cells. By tagging reactive phospholipids with fluorophores, we demonstrate modification of HeLa cell membranes with single-cell spatial resolution. Finally, we show that photo-triggered therapy is possible by coupling tetrazine photoactivation with strategies that release prodrugs in response to tetrazine ligation.

5G technology is the foundation for achieving intelligent mining construction. In response to the problem of difficult interoperability between mining 5G intelligent terminals and control platforms among different manufacturers, it is necessary to unify and standardize the data exchange and sharing process in the 5G system. The deployment architecture of '5G+intelligent mining' is proposed. The mining 5G intelligent terminals can interact and share data with servers containing control platforms through 5G base stations, switches, and 5G core networks in coal mines. This paper introduces the message types, message transmission formats, message composition, and message connection methods transmitted in the mining 5G system. For specific application scenarios in the mining field, the data in the mining 5G system is divided into voice data, video data, sensor data, and control data. The data types transmitted by the mining 5G intelligent terminal in different application scenarios are provided. This paper analyzes the basic transmission capabilities that mining 5G intelligent terminals and control platforms should have to meet data interaction. It is pointed out that the process of data interaction between mining 5G intelligent terminals and control platforms includes device information query and reporting, device upgrade, log collection, alarm issuance and processing, and platform configuration issuance and processing. It proposes the participating entities, security levels, and processes for data sharing of mining 5G intelligent terminals (including data aggregation, application, authorization, provision, and feedback). By implementing standardized processes, while ensuring communication reliability, the data exchange and sharing process under the '5G+intelligent mine' system architecture has been simplified to accelerate the intelligent construction of mines.

Chemical cross-linking enables rapid identification of RNA-protein and RNA-nucleic acid inter- and intramolecular interactions. However, no method exists to site-specifically and covalently cross-link two user-defined sites within an RNA. Here, we develop RNA-CLAMP, which enables site-specific and enzymatic cross-linking (clamping) of two selected guanine residues within an RNA. Intramolecular clamping can disrupt normal RNA function, whereas subsequent photocleavage of the cross-linker restores activity. We used RNA-CLAMP to clamp two stem loops within the single-guide RNA (sgRNA) of the CRISPR-Cas9 gene editing system via a photocleavable cross-linker, completely inhibiting gene editing. Visible light irradiation cleaved the cross-linker and restored gene editing with high spatiotemporal resolution. Design of two photocleavable linkers responsive to different wavelengths of light allowed multiplexed photoactivation of gene editing in mammalian cells. This photoactivated CRISPR-Cas9 gene editing platform benefits from undetectable background activity, provides a choice of activation wavelengths, and has multiplexing capabilities.