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Abstract: Immobilized phospholipid capillary electrophoresis (IPCE) was developed for studying the interactions between a set of nonsteroidal anti-inflammatory drugs (NSAIDs) and membrane and predicting the biological activity of NSAIDs. Supported vesicle layers and supported phospholipid bilayers were attached to the inner surface of a capillary wall simply by rinsing with liposome solutions. The liposomes, composed of soybean phosphatidylcholine (SPC) or SPC and different proportions of cholesterol (Ch), were small unilamellar vesicles prepared by sonication. The normalized capacity factor (K IPCE) was introduced into IPCE for evaluating drug–membrane interactions. Related theories and equations were derived to calculate K IPCE values from apparent migration time of a solute and electroosmotic flow. The strong relationships were observed between log K IPCE (SPC) values and log K lw values (the partition coefficients determined in free SPC-liposome partitioning system) (R =0.9855 and P <0.0001) or log K ILC values (the normalized capacity factors determined by immobilized POPC-liposome chromatography, POPC represents 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine) (R =0.9875 and P <0.0001). In addition, log K IPCE (SPC/Ch 80:20%) values correlated well with the pIC50 (the minus logarithm of IC50) values for cyclooxygenase 2 determined on intact cells (R =0.959 and P <0.001). These results confirmed that IPCE, K IPCE value as evaluation index, can be effectually used for studying drug–membrane interactions and it has the potential to predict drug activity. Cholesterol-containing (20mol%) liposomes may be more suitable to mimic real cell membrane. [Copyright &y& Elsevier]

Objective: To investigate the chemical constituents of the aerial parts of Plumbago zeylanica Linn., Methods: The constituents of the EtOAc-soluble portion in the 95% ethanol extract were isolated and purified by means of chromatography. Compounds were identified by their physical characteristics and spectral features., Results: Nine compounds were isolated as plumbagin (I), isoshinanolone (II), plumbagic acid (III), beta-sitosterol (IV), 4-hydroxybenzaldehyde (V), trans-cinnamic acid (VI), vanillic acid (VII), 2, 5-dimethyl-7-hydroxychromone (VIII), indole-3-carboxaldehyde (IX)., Conclusion: Compounds V, VII, VIII and IX were isolated for the first time from Plumbago Linn.