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19 results on '"Zhang, Shaobao"'

9. Rapid simultaneous determination of gut microbial phenylalanine, tyrosine, and tryptophan metabolites in rat serum, urine, and faeces using LC–MS/MS and its application to a type 2 diabetes mellitus study.

Author

Zou, Baorong, Sun, Yangwen, Xu, Zengmei, Chen, Yongda, Li, Lin, Lin, Lei, Zhang, Shaobao, Liao, Qiongfeng, and Xie, Zhiyong

Abstract

Gut microbial phenylalanine, tyrosine, and tryptophan metabolites are closely linked to various diseases. Monitoring the alterations of the related metabolites is vital to facilitate the understanding of pathophysiology of diseases. Herein, a rapid and sensitive assay based on LC–tandem mass spectrometry has been developed to analyze 20 gut microbial metabolites derived from phenylalanine, tyrosine, and tryptophan in rat serum, urine, and faeces. These microbial‐derived metabolites were separated on a phenyl‐hexyl column and simultaneously determined in a single run of 8 min. The detection limit for analytes ranged between 1.08 and 32.4 ng/mL. All calibration curves exhibited good linear relationships (R2 ≥ 0.9982). Intra‐ and inter‐assay precision values were below 15% and accuracies ranged from 85% to 115% for all analytes. The selectivity, matrix effect, and recovery of this method were all satisfactory. The validated method was successfully applied to characterize the alterations of these metabolites in type 2 diabetes mellitus rat. In general, the developed assay is suitable for high‐throughput monitoring of gut microbial phenylalanine, tyrosine, and tryptophan metabolites and provides a useful approach for exploring the mechanisms of microbial‐derived metabolites in diseases. [ABSTRACT FROM AUTHOR]

Published
2021
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11. A holistic view of gallic acid-induced attenuation in colitis based on microbiome-metabolomics analysis

Author

Li, Yuan, primary, Xie, Zhiyong, additional, Gao, Tingting, additional, Li, Lin, additional, Chen, Yongda, additional, Xiao, Dan, additional, Liu, Wen, additional, Zou, Baorong, additional, Lu, Biyu, additional, Tian, Xing, additional, Han, Bo, additional, Guo, Yaping, additional, Zhang, Shaobao, additional, Lin, Lei, additional, Wang, Mengxia, additional, Li, Pei, additional, and Liao, Qiongfeng, additional

Published
2019
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16. Simultaneous qualitative and quantitative determination of compounds in Aloe arborescens Mill using liquid chromatography with ion trap time‐of‐flight mass spectrometry and diode array detectors

Author

Li, Tiantian, primary, Liao, Ziqiong, additional, Chen, Mingyi, additional, Liu, Deliang, additional, Zhang, Shaobao, additional, Lin, Lei, additional, Zheng, Haihui, additional, Liao, Qiongfeng, additional, Xie, Zhiyong, additional, and Song, Fenyun, additional

Published
2018
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19. Shenling Baizhu San improves functional dyspepsia in rats as revealed by 1 H-NMR based metabolomics.

Author

Zhang S, Xu Z, Cao X, Xie Y, Lin L, Zhang X, Zou B, Liu D, Cai Y, Liao Q, and Xie Z

Subjects
Animals, Disease Models, Animal, Intestinal Absorption drug effects, Medicine, Chinese Traditional, Metabolic Networks and Pathways drug effects, Rats, Drugs, Chinese Herbal pharmacology, Drugs, Chinese Herbal therapeutic use, Dyspepsia therapy, Metabolomics, Proton Magnetic Resonance Spectroscopy
Abstract

Functional dyspepsia (FD), a common gastrointestinal disorder around the world, is driven by multiple factors, making prevention and treatment a major challenge. Shenling Baizhu San (SBS), a classical prescription of traditional Chinese medicine, has been proven to be effective in gastrointestinal disorders. However, studies on SBS improving FD are few. Thus, our study aimed to evaluate the effect of SBS on FD and further to explore the mechanism underlying the interactions between FD and SBS by the metabolomics approach. A FD rat model was induced by multiple forms of mild stimulation, and proton nuclear magnetic resonance (1H-NMR) spectroscopy and multivariate data analysis were used to profile the fecal and urinary metabolome in the FD rats during SBS intervention. Significant dyspeptic symptoms such as weight loss, poor appetite, reduced gastrointestinal motility and decreased absorptive capacity were observed in the FD rats, which were subsequently improved by SBS. Additionally, the levels of citrate, branched chain acids and pyruvate decreased, and the levels of choline, trimethylamine and taurine increased in the FD rats. Furthermore, the metabolic disorders were amended with SBS intervention mainly by modulating the metabolic pathways involved in energy metabolism, amino acid metabolism, and gut microbiota and host co-metabolism. Overall, our study highlighted the effect of SBS on the disturbed metabolic pathways in the FD rats, providing new insight into the mechanism of SBS treatment for FD from the perspective of metabolomics.

Published
2020
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