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168 results on '"Zhang, Tian-tuo"'

4. IL-38 alleviates airway remodeling in chronic asthma via blocking the profibrotic effect of IL-36γ.

Author

Zhang, Min, Zhou, Jian-Xia, Huang, Chu-Qin, Feng, Kang-Ni, Zou, Xiao-Ling, Cen, Jie-Mei, Meng, Ping, Li, Hong-Tao, and Zhang, Tian-Tuo

Subjects
HOUSE dust mites, ASTHMA, EXTRACELLULAR matrix, HUMAN migrations
Abstract

Airway remodeling is a major feature of asthma. Interleukin (IL)-36γ is significantly upregulated and promotes airway hyper-responsiveness (AHR) in asthma, but its role in airway remodeling is unknown. Here, we aimed to investigate the role of IL-36γ in airway remodeling, and whether IL-38 can alleviate airway remodeling in chronic asthma by blocking the effects of IL-36γ. IL-36γ was quantified in mice inhaled with house dust mite (HDM). Extracellular matrix (ECM) deposition in lung tissues and AHR were assessed following IL-36γ administration to mice. Airway inflammation, AHR, and remodeling were evaluated after IL-38 or blocking IL-36 receptor (IL-36R) treatment in asthmatic mice. The effects of lung fibroblasts stimulated with IL-36γ and IL-38 were quantified in vitro. Increased expression of IL-36γ was detected in lung tissues of HDM-induced asthmatic mice. The intratracheal instillation of IL-36γ to mice significantly enhanced the ECM deposition, AHR, and the number of activated lung fibroblasts around the airways. IL-38 or blocking IL-36R treated asthmatic mice showed a significant alleviation in the airway inflammation, AHR, airway remodeling, and number of activated fibroblasts around airways as compared with the HDM group. In vitro, IL-36γ promoted the activation and migration of human lung fibroblasts (HFL-1). The administration of IL-38 can counteract these biological processes induced by IL-36γ in HFL-1cells. The results indicated that IL-38 can mitigate airway remodeling by blocking the profibrotic effects of IL-36γ in chronic asthma. IL-36γ may be a new therapeutic target, and IL-38 is a potential candidate agent for inhibiting airway remodeling in asthma. IL-36γ was upregulated in the lungs of chronic asthma mice, and it contributed to the airway remodeling. IL-38 can alleviate the airway remodeling in asthma by regulating the effect of IL-36γ. Graphical Abstract [ABSTRACT FROM AUTHOR]

Published
2023
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14. Glucose-Potentiated Amikacin Killing of Cefoperazone/Sulbactam Resistant Pseudomonas aeruginosa

Author

Tang, Xi-kang, Su, Yu-bin, Ye, Hui-qing, Dai, Zhen-yuan, Yi, Huan, Yang, Ke-xin, Zhang, Tian-tuo, and Chen, Zhuang-gui

Subjects
Microbiology (medical), Microbiology
Abstract

Multidrug-resistant Pseudomonas aeruginosa has become one of global threat pathogens for human health due to insensitivity to antibiotics. Recently developed reprogramming metabolomics can identify biomarkers, and then, the biomarkers were used to revert the insensitivity and elevate antibiotic-mediated killing. Here, the methodology was used to study cefoperazone/sulbactam (SCF)-resistant P. aeruginosa (PA-RSCF) and identified reduced glycolysis and pyruvate cycle, a recent clarified cycle providing respiratory energy in bacteria, as the most key enriched pathways and the depressed glucose as one of the most crucial biomarkers. Further experiments showed that the depression of glucose was attributed to reduction of glucose transport. However, exogenous glucose reverted the reduction to elevate intracellular glucose via activating glucose transport. The elevated glucose fluxed to the glycolysis, pyruvate cycle, and electron transport chain to promote downstream proton motive force (PMF). Consistently, exogenous glucose did not promote SCF-mediated elimination but potentiated aminoglycosides-mediated killing since aminoglycosides uptake is PMF-dependent, where amikacin was the best one. The glucose-potentiated amikacin-mediated killing was effective to both lab-evolved PA-RSCF and clinical multidrug-resistant P. aeruginosa. These results reveal the depressed glucose uptake causes the reduced intracellular glucose and expand the application of metabolome-reprogramming on selecting conventional antibiotics to achieve the best killing efficacy.

Published
2022

15. Additional file 1 of IL-37 protects against airway remodeling by reversing bronchial epithelial–mesenchymal transition via IL-24 signaling pathway in chronic asthma

Author

Feng, Kang-ni, Meng, Ping, Zou, Xiao-ling, Zhang, Min, Li, Hai-ke, Yang, Hai-ling, Li, Hong-tao, and Zhang, Tian-tuo

Abstract

Additional file 1: Fig. S1. Interleukin-24 had no significant effect on proliferation, apoptosis, and cell cycle in BEAS-2B cells. (a) The effect of IL-24 on the cell viability of BEAS-2B cells was evaluated by CCK-8 assay followed by stimulation with different concentrations of IL-24 (0.1-100 ng/ml) for 24 h (n=5 wells / group). (b) The cells were stained with calcein AM and PI solution after treatment with 100 ng/ml IL-24 for 24 h. The green color indicates the live cells, and the red color represents the PI-positive nuclei. Scale bar=100 μm (×200). (c and d) After incubation with 10 or 100 ng/ml IL-24 for 24 h, flow cytometry was carried out to examine the cell apoptosis ratio. (e and f) The percentages of G0/G1, S and G2/M status were analyzed by flow cytometry after 10 or 100 ng/ml IL-24 treatment for 24 h. Bar diagrams and data are presented as the mean ± standard deviation (SD) from three replicate experiments. ns, no significant differences. Fig. S2. The effect of IL-24 and IL-37 on migration ability in BEAS-2B cells. (a) The effect of IL-24 on the migration of BEAS-2B cells was determined by wound healing assay. After stimulation with 100 ng/ml IL-24, scratches were captured at 0, 12 and 24 h. Scale bar=100 μm (×100). (b) Quantification of the wound healing assay showed the relative percentage of wound closure area. (c) The effect of IL-24 with or without IL-37 on the migration of BEAS-2B cells were evaluated by wound healing assay. After stimulation with 100 ng/ml IL-24 with or without 100 ng/ml IL-37, the scratches were captured at 0, 12 and 24 h. Scale bar=100 μm (×100). (d) Quantification of wound healing assay showed the percentage of wound closure area. Bar diagrams and data are presented as the mean ± standard deviation (SD) from three replicate experiments. * vs. control group; # vs. IL-24 group. *, #P < 0.05; **, ##P < 0.01; ***, ###P < 0.001.

Published
2022
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22. Lower neutrophil-to-lymphocyte ratio predicts high risk of multidrug-resistant Pseudomonas aeruginosa infection in patients with hospital-acquired pneumonia

Author

Zhou,Yu-Qi, Feng,Ding-Yun, Li,Wen-Juan, Yang,Hai-Ling, Wang,Zhao-Ni, Zhang,Tian-Tuo, and Chen,Zhuang-Gui

Subjects
Therapeutics and Clinical Risk Management, hospital-acquired pneumonia, risk factors, prognosis, multidrug-resistant Pseudomonas aeruginosa, Original Research
Abstract

Yu-Qi Zhou,1,2,* Ding-Yun Feng,1,2,* Wen-Juan Li,1,2 Hai-Ling Yang,1,2 Zhao-Ni Wang,3 Tian-Tuo Zhang,1,2 Zhuang-Gui Chen1,3 1Institute of Respiratory Diseases of Sun Yat-Sen University, Guangzhou, Guangdong, China; 2Department of Internal Medicine, Division of Respiratory Diseases, Guangzhou, Guangdong, China; 3Department of Pediatrics, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China *These authors contributed equally to this work Background and purpose: Hospital-acquired pneumonia (HAP) remains an important cause of morbidity and mortality despite advances in antimicrobial therapy. The emergence of multidrug resistant (MDR) Pseudomonas aeruginosa (PA) is of major concern. Our aim was to evaluate the risk factors and prognosis of HAP due to MDR-PA infection.Patients and methods: In a retrospective observational study, we collected data on all episodes of HAP caused by PA (PA-HAP) occurring from January 2013 to December 2016. Characteristics of patients with drug-sensitive PA were compared with those with MDR-PA. Data of demographic, underlying conditions, peripheral neutrophil-to-lymphocyte ratio (NLR), and clinical outcomes were collected and analyzed.Results: One hundred fifty-seven patients with PA-HAP were included, of which 69 (43.9%) patients were diagnosed with MDR-PA infection. There were significant differences between MDR-PA group and non-MDR-PA group on the following variables: initial inappropriate antibiotic therapy (P

Published
2018

36. Selection of glucocorticoid-sensitive patients in interstitial lung disease secondary to connective tissue diseases population by radiomics

Author

Feng, Ding-Yun, primary, Zhou, Yu-Qi, additional, Xing, Yan-Fang, additional, Li, Chuang-Feng, additional, Lv, Qing, additional, Dong, Jie, additional, Qin, Jie, additional, Guo, Yue-Fei, additional, Jiang, Nan, additional, Huang, Chencui, additional, Hu, Hai-Tao, additional, Guo, Xing-Hua, additional, Chen, Jie, additional, Yin, Liang-Hong, additional, Zhang, Tian-Tuo, additional, and Li, Xing, additional

Published
2018
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42. Thymic stromal lymphopoietin contribution to the recruitment of circulating fibrocytes to the lung in a mouse model of chronic allergic asthma.

Author

Chen, Zhuang-Gui, Meng, Ping, Li, Hong-Tao, Li, Ming, Yang, Li-Fen, Yan, Yan, Li, Ya-Ting, Zou, Xiao-Ling, Wang, De-Yun, and Zhang, Tian-Tuo

Subjects
THYMIC stromal lymphopoietin, FIBROBLASTS, BRONCHOALVEOLAR lavage, CONFOCAL microscopy, ASTHMA
Abstract

Objective: Fibrocyte localization to the airways and thymic stromal lymphopoietin (TSLP) overexpression in the lung are features of severe asthma. The aim of this study was to determine whether TSLP contributes to fibrocyte trafficking and airway remodeling in a mouse model of allergic asthma. Methods: We established a chronic asthma animal model by administering house dust mite (HDM) extracts intranasally for up to 5 consecutive weeks. Mouse anti-TSLP monoclonal antibody (mAb) was given intraperitoneally starting the 4th week. Fluorescence-labeled CD34/collagen I (Col I)-dual-positive fibrocytes were examined by confocal microscopy. The level of TGF-β1 in the bronchoalveolar lavage (BAL) fluid was determined by ELISA. Results: We found significantly increased levels of TSLP and TGF-β1 in the lung of the mice subjected to repeated allergen exposure, which was accompanied by increased number of fibrocytes in the sub-epithelial zone and the BAL fluid. However, blocking TSLP markedly decreased the production of TGF-β1, reduced the number of fibrocytes and subsequently prevented alterations of both airway and vascular structures. Conclusions: Our data suggested that TSLP might function in airway remodeling by promoting circulating fibrocyte recruitment to the lung in the mice subjected to chronic allergen exposure. These results provide a better rationale for targeting the interaction between TSLP and fibrocytes as a therapeutic approach for chronic allergic asthma. [ABSTRACT FROM AUTHOR]

Published
2018
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43. Lower neutrophil-to-lymphocyte ratio predicts high risk of multidrug-resistant infection in patients with hospital-acquired pneumonia.

Author

Zhou, Yu-Qi, Feng, Ding-Yun, Li, Wen-Juan, Yang, Hai-Ling, Wang, Zhao-Ni, Zhang, Tian-Tuo, and Chen, Zhuang-Gui

Subjects
NOSOCOMIAL infections, PSEUDOMONAS aeruginosa infections, ANTIBIOTICS, MULTIDRUG resistance in bacteria, PNEUMONIA-related mortality
Abstract

Background and Purpose: Hospital-acquired pneumonia (HAP) remains an important cause of morbidity and mortality despite advances in antimicrobial therapy. The emergence of multidrug resistant (MDR) Pseudomonas aeruginosa (PA) is of major concern. Our aim was to evaluate the risk factors and prognosis of HAP due to MDR-PA infection.Patients and Methods: In a retrospective observational study, we collected data on all episodes of HAP caused by PA (PA-HAP) occurring from January 2013 to December 2016. Characteristics of patients with drug-sensitive PA were compared with those with MDR-PA. Data of demographic, underlying conditions, peripheral neutrophil-to-lymphocyte ratio (NLR), and clinical outcomes were collected and analyzed.Results: One hundred fifty-seven patients with PA-HAP were included, of which 69 (43.9%) patients were diagnosed with MDR-PA infection. There were significant differences between MDR-PA group and non-MDR-PA group on the following variables: initial inappropriate antibiotic therapy (P<0.001, OR 0.103, 95% CI 0.044-0.244), admission in more than two departments in previous 30 days (P<0.001, OR 0.186, 95% CI 0.072-0.476), and NLR level (P=0.020, OR 0.911, 95% CI 0.843-0.985). The effect of antibiotic treatment was significantly different (P<0.001, OR 4.263, 95% CI 2.142-8.483). The 30-day mortality was higher in MDR-PA group than that in non-MDR-PA group (P<0.001).Conclusion: We have shown that lower NLR level was identified as a clinical predictor of MDR-PA infection in HAP patients. Even with goal-directed therapy, MDR-PA infection implicates poor outcomes in patients with HPA. [ABSTRACT FROM AUTHOR]

Published
2018
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50. A Case of Human Fascioliasis Diagnosed by Serological Method

Author

DOUTSU, Yasumasa, primary, ZHANG, Tian-tuo, additional, NISHIHATA, Shinji, additional, FUKUSHIMA, Kiyoyasu, additional, MISHIMA, Yukinobu, additional, HAYASHI, Toshiaki, additional, KOHNO, Shigeru, additional, IMANISHI, Takeo, additional, NAKAMURA, Noriaki, additional, YAMAGUCHI, Keizo, additional, HIROTA, Masaki, additional, HARA, Kohei, additional, TSUKIDATE, Setsuko, additional, FUJITA, Koichiro, additional, and ARAKI, Kunioki, additional

Published
1988
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