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Low oxygen and temperature pose key physiological challenges for endotherms living on the Qinghai-Tibetan Plateau ( QTP). Molecular adaptations to high-altitude living have been detected in the genomes of Tibetans, their domesticated animals and a few wild species, but the contribution of transcriptional variation to altitudinal adaptation remains to be determined. Here we studied a top QTP predator, the saker falcon, and analysed how the transcriptome has become modified to cope with the stresses of hypoxia and hypothermia. Using a hierarchical design to study saker populations inhabiting grassland, steppe/desert and highland across Eurasia, we found that the QTP population is already distinct despite having colonized the Plateau <2000 years ago. Selection signals are limited at the cDNA level, but of only seventeen genes identified, three function in hypoxia and four in immune response. Our results show a significant role for RNA transcription: 50% of upregulated transcription factors were related to hypoxia responses, differentiated modules were significantly enriched for oxygen transport, and importantly, divergent EPAS1 functional variants with a refined co-expression network were identified. Conservative gene expression and relaxed immune gene variation may further reflect adaptation to hypothermia. Our results exemplify synergistic responses between DNA polymorphism and RNA expression diversity in coping with common stresses, underpinning the successful rapid colonization of a top predator onto the QTP. Importantly, molecular mechanisms underpinning highland adaptation involve relatively few genes, but are nonetheless more complex than previously thought and involve fine-tuned transcriptional responses and genomic adaptation. [ABSTRACT FROM AUTHOR]

The ability to detect bitter tastes is important for animals; it can help them to avoid ingesting harmful substances. Bitter taste perception is mainly mediated by bitter taste receptor proteins, which are encoded by members of the Tas2r gene family and vary with the dietary preference of a specific species. Although individuals with different genotypes differ in bitterness recognition capability, little is known about the relationship between genetic variation and food selection tendencies at the intraspecific level. In this study, we examined the relationship between genotypes and diet in plateau zokor ( Eospalax baileyi), a subterranean rodent endemic to the Qinghai-Tibet Plateau that caches food for the winter. We assayed the composition and taste profile of each plant contained in temporary caches and vicinity quadrats, which were representative of selected and available food, respectively. Bitter plant selection indices ( Ebitter) were estimated. We also sequenced 26 candidate Tas2r genes from zokors and determined their relationships with the Ebitter of their caches. We identified four key results: (1) zokors varied considerably in both bitter food preference and Tas2r sequences; (2) five genes ( zTas2r115, zTas2r119, zTas2r126, zTas2r134, and zTas2r136) exhibited allelic variation that was significantly associated with Ebitter; (3) synonymous SNPs, nonsynonymous SNPs, and pseudogenization are involved in the genotype-phenotype relationship; (4) the minor genotypes of zTas2r115, zTas2r134, and zTas2r136 and the major genotypes of zTas2r119 and zTas2r126 cached more bitter plants. Our results link Tas2r variation with food selection behavior at the population level for the first time. [ABSTRACT FROM AUTHOR]

Abstract: Since the first description of the Bergmann''s rule, body size clines along environmental gradients have been examined in a wide variety of taxa. Broad support for Bergmann''s rule has been found for endotherms and even ectotherms; many species, however, do not follow Bergmann''s rule or the converse to Bergmann''s rule. We tested the relationship between body size (body weight (BW), body length (BL), and 12 skull size measurements) of a typical subterranean rodent plateau zokor (Eospalax baileyi) collected from different geographic localities and two geographic variables (latitude and altitude) as well as some environmental factors that usually change with geographical gradients on the Qinghai-Tibetan Plateau. A total of 523 (212 males and 311 females) adult individuals from 21 sampling sites were analyzed. The results indicated that body size of both males and females was positively correlated with latitude, annual temperature (AT), and annual net primary production (ANPP) and negatively correlated with altitude and annual precipitation (AP). These results indicated that the plateau zokor latitudinally followed Bergmann''s rule but altitudinally followed the converse to Bergmann''s rule. The environmental factors which may influence the zokors’ water balance and food availability were the major driving forces latitudinally and altitudinally shaping the body size of this species, respectively. [Copyright &y& Elsevier]

Abstract: Large group sizes have been hypothesized to decrease predation risk and increase food competition. We investigated group size effects on vigilance and foraging behaviour during the migratory period in female Tibetan antelope Pantholops hodgsoni, in the Kekexili Nature Reserve of Qinghai Province, China. During June to August, adult female antelope and yearling females gather in large migratory groups and cross the Qinghai–Tibet highway to calving grounds within the Nature Reserve and return to Qumalai county after calving. Large groups of antelope aggregate in the migratory corridor where they compete for limited food resources and attract the attention of mammalian and avian predators and scavengers. We restricted our sampling to groups of less than 30 antelopes and thus limit our inference accordingly. Focal-animal sampling was used to record the behaviour of the free-ranging antelope except for those with lambs. Tibetan antelope spent more time foraging in larger groups but frequency of foraging bouts was not affected by group size. Conversely, the time spent vigilant and frequency of vigilance bouts decreased with increased group size. We suggest that these results are best explained by competition for food and risk of predation. [Copyright &y& Elsevier]

Shorebirds are facing global decline as a consequence of anthropogenic effects that include habitat loss, increased predation and climate change. Although some shorebirds are extensively studied, basic data on life history, ecology and behaviour are still lacking especially for populations where climate change is expected to have severe effects. Here, we provide the first detailed investigation of a high-altitude nesting shorebird in the Qinghai–Tibetan Plateau, the Tibetan Sand Plover Charadrius atrifrons. This alpine-breeding Central-Asian species recently split from the Lesser Sand Plover Charadrius mongolus that breeds in Eastern Siberia. Understanding the fundamental ecology and behaviour of alpine-breeding shorebirds is important for predicting the challenges these high-altitude species will face given the human-driven environmental changes that are likely to take place in these habitats. Here, we show that Tibetan Sand Plovers in the Qinghai–Tibetan Plateau have a short breeding season from mid-May to late-June, and exhibit social monogamy and bi-parental care of the clutch. Since they have low hatching success (18 of 57 nests hatched) and no chance of renesting, maintaining population size in the future may pose a challenge. To provide essential information for protection of this species in a vulnerable habitat, further demographic studies are warranted to better understand how climate change will mould adaptations of this specialised high-altitude species to their environment. [ABSTRACT FROM AUTHOR]

Simple Summary: The gut microbiota and metabolites play a crucial role in the physiological health of the host and are influenced by various factors, including seasons. We utilized 16s sequencing and untargeted metabolomics to uncover the impact of seasonal change on the gut microbiota and metabolites of Tibetan antelopes. Overall, this study revealed differences in the gut microbiota and metabolites of Tibetan antelopes during the cold and warm seasons. These findings can provide a basis for the conservation of Tibetan antelopes. The Tibetan antelope is an endangered species suffering from poaching and habitat fragmentation. The intestinal flora and metabolites play a crucial role in the physiological homeostasis of hosts, which are influenced by various environmental factors like seasonal variation. In this particular research, our main goal was to explore the alterations in the metabolism and gut microbiota of Tibetan antelopes between the cold season (XB) and warm season (DA), using untargeted metabolomics and 16S rRNA gene-sequencing analyses. The findings indicated that Tibetan antelopes had a higher alpha-diversity of intestinal microbes during the cold season than during the warm season. Principal co-ordinate analysis revealed notable seasonal discrepancies in the function and structure of intestinal microbes in Tibetan antelopes. The relative abundance of Firmicutes was significantly increased during the cold season compared to during the warm season. Furthermore, the Tibetan antelope's primary metabolic functions of the intestinal micro-organisms were significantly higher during the cold season. The untargeted metabolomics analysis results showed a total of 532 metabolites that were significantly different between the cold season and warm season groups. These metabolites were found to be enriched in a total of 62 metabolic pathways. Among the most significant pathways of enrichment were the purine metabolism and pyrimidine metabolism. The levels of related metabolites in those pathways were remarkably higher in the warm season compared to the cold season. The comprehensive analysis of 16S rRNA and the metabolome reveals there is a significant correlation between differential microbiota and differential metabolites. Therefore, the gut microbiota changes caused by seasonal changes influenced the metabolites as well. This research reveals the function of seasonal changes in the intestinal flora and metabolites in the adaptation of Tibetan antelopes to environmental fluctuations and supplies a theoretical basis for instructing the protection management of Tibetan antelopes. [ABSTRACT FROM AUTHOR]

Ex situ conservation is the main method for the protection of endangered wildlife. To explore the effect of ex situ conservation on the gut microbiota of the kiang (Equus kiang), metagenomic sequencing combined with bioinformatics analysis was used to investigate the composition and function of the gut microbiota of the kiang. The results showed that ex situ conservation not only protected wildlife, but also affected the composition and function of gut microbiota, as well as the health of animals. In the zoo, the ratio of the relative abundance of Firmicutes to that of Bacteroidetes (F/B) is higher, clusters of potentially pathogenic bacteria (such as Catonella, Catonella, and Mycoplasma) are more numerous, the abundance of resistance genes is higher, and the abundance of metabolic functions is increased. The dynamic changes of the gut microbiota also played an important role in the nutritional absorption, energy metabolism, and environmental adaptation of the kiang. Improving the rearing environment and increasing food diversity play important roles for increasing the diversity of gut microbiota, reducing the spread of potentially pathogenic bacteria, and reducing diseases. In the wild, especially in winter and in food‐deficient areas, food supplementation can enhance the gut microbial homeostasis of wild animals and reduce the impact of crises. In depth studies of the gut microbial function of wildlife have important implications for improving ex situ conservation. [ABSTRACT FROM AUTHOR]

Background: The oriental rat flea (Xenopsylla cheopis), which infests several mammals, primarily rats (Rattus spp.), is the most notorious vector of human plague. In this study, we measured the genetic differentiation among populations of fleas from the Asian house rat (Rattus tanezumi) and the brown rat (R. norvegicus) using microsatellite markers in order to investigate the extent of host-switching in this parasite. Results: We developed 11 polymorphic microsatellite loci for our study, nine of which showed high potential for inbreeding. AMOVA showed that the majority (84.07%, P < 0.001) of the variation was derived from within populations, followed by variation among groups (14.96%, P < 0.001); in contrast, variation within groups of populations was nearly absent (0.97%, P > 0.05). Analyses of the pairwise fixation index revealed that most of the ten allopatric population pairs but none of the five sympatric population pairs were significantly differentiated. Moreover, based on genetic structure clustering analysis, there was obvious differentiation between allopatric populations but not between sympatric population pairs. Conclusions: These results indicate the presence of frequent migrations of the oriental rat flea between the sympatric Asian house rat and brown rat, causing a high rate of gene flow and limited genetic differentiation. We suggest that there is no clear boundary limiting the migration of oriental rat fleas between the two hosts, and thus both rat species should be monitored equally for the purposes of plague prevention and control. [ABSTRACT FROM AUTHOR]

Bamboo rats are a group of subterranean rodents some of which feed on the roots and shoots of bamboo and other plants. In this study, we sequence the mitochondrial genome of a hoary bamboo rat Rhizomys pruinosus from the south of China. The genome is 16,575 bp in length, and had a gene content of 13 protein coding, 22 tRNAs and 2 rRNAs. The overall base composition is 30.94% T, 24.83% C, 32.21% A and 12.02% G, with an A + T bias of 63.15%. The cytochrome genes were the most conservative genes compared with plateau zokor ( Eospalax baileyi) and blind mole rats ( Spalax carmeli) in the family Spalacidae. These mitochondrial data are potentially important for the study of molecular evolution, conservation genetics, agricultural technology and epidemiology. [ABSTRACT FROM AUTHOR]

Maintaining a healthy status is crucial for the successful captive breeding of endangered alpine musk deer (Moschus chrysogaster, AMD), and captive breeding programs are beneficial to the ex-situ conservation and wild population recovery of this species. Meanwhile, the gut microbiota is essential for host health, survival, and environmental adaptation. However, changes in feeding environment and food can affect the composition and function of gut microbiota in musk deer, ultimately impacting their health and adaptation. Therefore, regulating the health status of wild and captive AMD through a non-invasive method that targets gut microbiota is a promising approach. Here, 16S rRNA gene sequencing was employed to reveal the composition and functional variations between wild (N = 23) and captive (N = 25) AMD populations. The results indicated that the gut microbiota of wild AMD exhibited significantly higher alpha diversity (P < 0.001) and greater abundance of the phylum Firmicutes, as well as several dominant genera, including UCG-005, Christensenellaceae R7 group, Monoglobus, Ruminococcus, and Roseburia (P < 0.05), compared to captive AMD. These findings suggest that the wild AMD may possess more effective nutrient absorption and utilization, a more stable intestinal microecology, and better adaption to the complex natural environment. The captive individuals displayed higher metabolic functions with an increased abundance of the phylum Bacteroidetes and certain dominant genera, including Bacteroides, Rikenellaceae RC9 gut group, NK4A214 group, and Alistipes (P < 0.05), which contributed to the metabolic activities of various nutrients. Furthermore, captive AMD showed a higher level of 11 potential opportunistic pathogens and a greater enrichment of disease-related functions compared to wild AMD, indicating that wild musk deer have a lower risk of intestinal diseases and more stable intestinal structure in comparison to captive populations. These findings can serve as a valuable theoretical foundation for promoting the healthy breeding of musk deer and as a guide for evaluating the health of wild-released and reintroduced musk deer in the future. Key points: • Wild and captive AMD exhibit contrasting gut microbial diversity and certain functions. • With higher diversity, certain bacteria aid wild AMD's adaptation to complex habitats. • Higher potential pathogens and functions increase disease risk in captive AMD. [ABSTRACT FROM AUTHOR]

Over the past 40 years, the climate has been changing and human disturbance has increased in the vast Qinghai–Tibet Plateau (QTP). These 2 factors are expected to affect the distribution of a large number of endemic vertebrate species. However, quantitative relationships between range shifts and climate change and human disturbance of these species in the QTP have rarely been evaluated. We used occurrence records of 19 terrestrial vertebrate species (birds, mammals, amphibians, and reptiles) occurring in the QTP from 1980 to 2020 to quantify the effects of climate change and anthropogenic impacts on the distribution of these 4 taxonomic groups and estimated species range changes in each species. The trend in distribution changes differed among the taxonomic groups, although, generally, ranges shifted to central QTP. Climate change contributed more to range variation than human disturbance (the sum of the 4 climatic variables contributed more than the sum of the 4 human disturbance variables for all 4 taxonomic groups). Suitable geographic range increased for most mammals, amphibians, and reptiles (+27.6%, +18.4%, and +27.8% on average, respectively), whereas for birds range decreased on average by 0.9%. Quantitative evidence for climate change and human disturbance associations with range changes for endemic vertebrate species in the QTP can provide useful insights into biodiversity conservation under changing environments. [ABSTRACT FROM AUTHOR]

This paper presents an autonomous unmanned-aerial-vehicle (UAV) tracking system based on an improved long and short-term memory (LSTM) Kalman filter (KF) model. The system can estimate the three-dimensional (3D) attitude and precisely track the target object without manual intervention. Specifically, the YOLOX algorithm is employed to track and recognize the target object, which is then combined with the improved KF model for precise tracking and recognition. In the LSTM-KF model, three different LSTM networks (f, Q, and R) are adopted to model a nonlinear transfer function to enable the model to learn rich and dynamic Kalman components from the data. The experimental results disclose that the improved LSTM-KF model exhibits higher recognition accuracy than the standard LSTM and the independent KF model. It verifies the robustness, effectiveness, and reliability of the autonomous UAV tracking system based on the improved LSTM-KF model in object recognition and tracking and 3D attitude estimation. [ABSTRACT FROM AUTHOR]

Sanjiangyuan National Park (SNP), the first national park in China, is one of the most important biodiversity conservation areas in the Sanjiangyuan National Nature Reserve (SNNR) and even the world. The threatened ungulates play an irreplaceable role in maintaining the ecosystem diversity and stability in SNNR. Here, based on 1434 occurrence records of six ungulates, the maximum entropy model, with two different strategies, was utilized to determine the priority reserves. The results indicated that the priority reserves in SNNR was mainly located in and around SNP, which were mainly distributed in the middle east, middle west, and southwest of SNNR. Six ungulates shared preference for altitude ranging 4000–5000 m, the average annual temperature below −3.0°C, and average annual precipitation ranging 200–400 mm on meadow, steppe, and unused land. The proportion of high and medium suitable areas for ungulates in SNP was higher than that in SNNR. As the SNP is not contiguously spaced in space, and some core wildlife habitats are not included, it is suggested to optimize the functional areas and adjust the boundary range on the basis of the pilot scope of SNP, so as to enhance the integrity and connectivity of each functional area. [ABSTRACT FROM AUTHOR]

In high-altitude environments, the prevalence of high-altitude polycythemia (HAPC) ranges between 5 and 18 percent. However, there is currently no effective treatment for this condition. Therefore, disease prevention has emerged as a critical strategy against this disease. Here, we looked into the microarray profiles of GSE135109 and GSE29977, linked to either short- or long-term exposure to the Qinghai Tibet Plateau (QTP). The results revealed inhibition in the adaptive immune response during 30 days of exposure to QTP. Following a gene set enrichment analysis (GSEA) discovered that genes associated with HAPC were enriched in Cluster1, which showed a dramatic upregulation on the third day after arriving at the QTP. We then used GeneLogit to construct a logistic prediction model, which allowed us to identify 50 genes that classify HAPC patients. In these genes, LRRC18 and HCAR3 were also significantly altered following early QTP exposure, suggesting that they may serve as hub genes for HAPC development. The in-depth study of a combination of the datasets of transcriptomic changes during exposure to a high altitude and whether diseases occur after long-term exposure in Hans can give us some inspiration about genes associated with HAPC development during adaption to high altitudes. [ABSTRACT FROM AUTHOR]

Gut microbiota forms a unique microecosystem and performs various irreplaceable metabolic functions for ruminants. The gut microbiota is important for host health and provides new insight into endangered species conservation. Forest musk deer (FMD) and alpine musk deer (AMD) are typical small ruminants, globally endangered due to excessive hunting and habitat loss. Although nearly 60 years of captive musk deer breeding has reduced the hunting pressure in the wild, fatal gastrointestinal diseases restrict the growth of captive populations. In this study, 16S rRNA high-throughput sequencing revealed the differences in gut microbiota between FMD and AMD based on 166 fecal samples. The alpha diversity was higher in FMD than in AMD, probably helping FMD adapt to different and wider habitats. The ß-diversity was higher between adult FMD and AMD than juveniles and in winter than late spring. The phylum Firmicutes and the genera Christensenellaceae R7 group, Ruminococcus, Prevotellaceae UCG-004, and Monoglobus were significantly higher in abundance in FMD than in AMD. However, the phylum Bacteroidetes and genera Bacteroides, UCG-005, Rikenellaceae RC9 gut group, and Alistipes were significantly higher in AMD than FMD. The expression of metabolic functions was higher in AMD than in FMD, a beneficial pattern for AMD to maintain higher energy and substance metabolism. Captive AMD may be at higher risk of intestinal diseases than FMD, with higher relative abundances of most opportunistic pathogens and the expression of disease-related functions. These results provide valuable data for breeding healthy captive musk deer and assessing their adaptability in the wild. Key points: • Alpha diversity of gut microbiota was higher in FMD than that in AMD • Expression of metabolic and disease-related functions was higher in AMD than in FMD [ABSTRACT FROM AUTHOR]

[Display omitted] • Revolutionizing National Park zoning in China with keystone species. • Habitat suitability of 16 wildlife in Kunlun area exhibits heterogeneity with MaxEnt. • KMNP's boundary and functional zoning were optimized, outperforming the selected area. National parks in China play a vital role in preserving biodiversity, protecting endangered species, maintaining ecological balance, and promoting sustainable development amid global biodiversity decline. Boundary delimitation and functional zoning within these parks are crucial for preserving ecosystem integrity and species diversity. In the case study of Kunlun Mountains National Park (KMNP, Qinghai section), an extensive wildlife background survey yielded 4,523 occurrence records (3,921 after optimization) for 16 nationally protected wildlife species. Using the ecological niche model, this study aimed to explore the boundary range, functional zoning, and protection gap areas within KMNP by simulating the suitable spatial distribution of wildlife species, taking into account their protection and endangered statuses. The findings indicated that the primary habitat for key protected species in KMNP mainly occupied meadow and steppe in terms of vegetation types, along with grassland and unused land in terms of land use types, with average annual precipitation from 200 mm to 400 mm, elevations ranging from 4,100 m to 4,700 m, and average annual temperatures from −4.5℃ to −2℃. The suitable distribution of different wildlife species in the Kunlun Mountains area showed variations, mainly concentrated in the southwestern, central, and southeastern regions. The optimized KMNP covered around 49,800 km2, a 16.3 % reduction from the selected area, with the core protection zone at 16,300 km2 and the general control zone at 33,200 km2. The suitability of 93.75 % (15/16) species within the core protection area of KMNP was higher comparing to the optimized area, selected area, and Mangya-Golmud-Dulan area, indicating that the boundary delimitation and functional zoning of KMNP were highly rational. The boundary range and functional zoning method developed in this study provide a crucial scientific foundation for KMNP's conservation and management, and valuable guidance for national park construction, ecosystem preservation, biodiversity conservation, and the promotion of key species' reproduction and survival on the Qinghai-Tibet Plateau, with promising future applications. [ABSTRACT FROM AUTHOR]

• A human-wildlife spatial interaction index was proposed. • The average level of human-wildlife interaction in the study area was low. • Higher potential human-wildlife interaction occurred in areas with roads and rails. In the context of today's rapid economic development and increasingly strict ecological protection, interactions between humans and wildlife will intensify. Therefore, we need to identify the spatial distribution of such interactions and to take measures to mitigate possible negative interactions. Using the Three-Rivers Source Region as a case study, we combined species habitat suitability, species feeding habits, species conservation levels and human impact intensity to construct a "human-wildlife spatial interaction index" (HWSII) to evaluate the intensity of human-wildlife spatial interactions. The overall level of HWSII was relatively low in the Three-River Source Region. Higher potential human-wildlife interactions occurred in areas with roads and railways. Areas of relatively high interactions between carnivores and livestock were mainly located in the southern part of the study area, and those with relatively high interactions between herbivores and livestock appeared in the western and eastern parts. Managers in these areas should consider mitigation measures. Although developed for a specific region of China, the 'human-wildlife spatial interaction index' could have application in other areas of the world for setting a baseline reference to identify areas with the potential for high levels of human-wildlife interactions and pinpoint places where potential negative interactions can be mitigated. [ABSTRACT FROM AUTHOR]

Background: The spleen is the largest secondary lymphoid organ and the main site where stress erythropoiesis occurs. It is known that hypoxia triggers the expansion of erythroid progenitors; however, its effects on splenic gene expression are still unclear. Here, we examined splenic global gene expression patterns by time-series RNA-seq after exposing mice to hypoxia for 0, 1, 3, 5, 7 and 13 days. Results: Morphological analysis showed that on the 3rd day there was a significant increase in the spleen index and in the proliferation of erythroid progenitors. RNA-sequencing analysis revealed that the overall expression of genes decreased with increased hypoxic exposure. Compared with the control group, 1380, 3430, 4396, 3026, and 1636 genes were differentially expressed on days 1, 3, 5, 7 and 13, respectively. Clustering analysis of the intersection of differentially expressed genes pointed to 739 genes, 628 of which were upregulated, and GO analysis revealed a significant enrichment for cell proliferation. Enriched GO terms of downregulated genes were associated with immune cell activation. Expression of Gata1, Tal1 and Klf1 was significantly altered during stress erythropoiesis. Furthermore, expression of genes involved in the immune response was inhibited, and NK cells decreased. Conclusions: The spleen of mice conquer hypoxia exposure in two ways. Stress erythropoiesis regulated by three transcription factors and genes in immune response were downregulated. These findings expand our knowledge of splenic transcriptional changes during hypoxia. [ABSTRACT FROM AUTHOR]

Wild-caught animals must cope with drastic lifestyle and dietary changes after being induced to captivity. How the gut microbiome structure of these animals will change in response receives increasing attention. The plateau zokor (Eospalax baileyi), a typic subterranean rodent endemic to the Qinghai-Tibet plateau, spends almost the whole life underground and is well adapted to the environmental pressures of both plateau and underground. However, how the gut microbiome of the plateau zokor will change in response to captivity has not been reported to date. This study compared the microbial community structure and functions of 22 plateau zokors before (the WS group) and after being kept in captivity for 15 days (the LS group, fed on carrots) using the 16S rRNA gene via high-throughput sequencing technology. The results showed that the LS group retained 973 of the 977 operational taxonomic units (OTUs) in the WS group, and no new OTUs were found in the LS group. The dominant bacterial phyla were Bacteroides and Firmicutes in both groups. In alpha diversity analysis, the Shannon, Sobs, and ACE indexes of the LS group were significantly lower than those of the WS group. A remarkable difference (P < 0.01) between groups was also detected in beta diversity analysis. The UPGMA clustering, NMDS, PCoA, and Anosim results all showed that the intergroup difference was significantly greater than the intragroup difference. And compared with the WS group, the intragroup difference of the gut microbiota in the LS group was much larger, which failed to support the assumption that similar diets should drive convergence of gut microbial communities. PICRUSt revealed that although some functional categories displayed significant differences between groups, the relative abundances of these categories were very close in both groups. Based on all the results, we conclude that as plateau zokors enter captivity for a short time, although the relative abundances of different gut microbiota categories shifted significantly, they can maintain almost all the OTUs and the functions of the gut microbiota in the wild. So, the use of wild-caught plateau zokors in gut microbial studies is acceptable if the time in captivity is short. [ABSTRACT FROM AUTHOR]

Overlap between wildlife living spaces and human activity spaces represents one of the main causes of human-wildlife conflict. It is therefore necessary to identify and adjust the spatial distribution of conflicts because climate change will modify the ranges and locations of species. The MaxEnt model was used to simulate the distribution of wildlife living spaces under current and future climatic conditions (RCP4.5 scenario) based on species distribution data and environmental data from the Three-River Source Region. Furthermore, the logistic-cellular automata model was used to simulate the changes in human activity spaces (construction land and arable land) by 2050. Areas of overlap between wildlife living spaces and human activity spaces were determined by overlay analysis. Then, the areas of construction and arable land that would conflict with wildlife were redistributed outside highly suitable wildlife living spaces. In addition, ecological corridors connecting current and future living spaces were planned for the migration of certain species in response to climate change based on the minimum cumulative resistance model. The results showed that the areas of highly suitable wildlife living spaces will decrease under the influence of climate change if global warming occurs. In the future, the area of overlap between highly suitable wildlife living spaces and construction land will be 125 km2, and that between highly suitable wildlife living spaces and arable land will be 340 km2. The redistribution of these areas will cause human activity spaces to become more concentrated. Moreover, 110 ecological corridors should be constructed across roads in the Three-River Source Region. • The increase or decrease in spatial conflict areas will vary from species to species. • Construction land and arable land will become more concentrated after adjustment. • The number of corridors for species migration from current to future will be 110. [ABSTRACT FROM AUTHOR]

Upland buzzard (Buteo hemilasius), Saker falcon (Falco cherrug), and Himalayan vulture (Gyps himalayensis) are three common large raptors in the Sanjiangyuan National Park (SNP), China's first national park. Among them, Upland buzzard and Saker falcon play a significant role in controlling plateau rodent populations and reducing the transmission of pathogens carried by rodents. The Himalayan vulture can provide services for the redistribution and recycling of nutrients in the ecosystem, and play an irreplaceable role in the celestial burial culture of Tibetans in China. Exploring their habitat suitability is important for the protection of the three raptors. Our research was based on the current distribution of Upland buzzard, Saker falcon, and Himalayan vulture that we had extensively surveyed in the Sanjiangyuan National Park from 2016 to 2017. Combined with the correlation analysis of environmental variables, we utilized maximum entropy model (MaxEnt) to evaluate and compare the habitat suitability of the three species in the Sanjiangyuan National Park. Elevation, climate, and human disturbance factors, which had direct or indirect effects on species survival and reproduction, were all included in the model. Among them, elevation was the most important environmental variables affecting the suitability of habitats of three species. Temperature‐related factor was another important predictor. The high (>60%) suitable habitat areas for Upland buzzard, Saker falcon, and Himalayan vulture were 73,017.63, 40,732.78, and 61,654.33 km2, respectively, accounted for 59.32%, 33.09%, and 50.08% of the Sanjiangyuan National Park and their total suitable area (i.e., the sum area of high and moderate habitats) reached 96.07%, 60.59%, and 93.70%, respectively. Besides, the three species have overlapping areas for the suitable habitats, which means that overlapping areas should be highly valued and protected. Therefore, understanding the distribution of suitable habitats of the three raptors can provide useful information and reasonable reference for us to put forward suggestions for their protection and regional management. [ABSTRACT FROM AUTHOR]

Lithobius (Ezembius) polyommatus sp. n. and Lithobius (Ezembius) dulanensis sp. n. discovered from Qinghai-Tibet Plateau, China, are described. The two new species are morphologically similar, but could be distinguished by having mostly 12 ocelli other than 14 ocelli, anterior accessory spur absent versus present on legs 14, dorsal sulci on male 14th legs absent versus present and dorsal lateral setae short, only the largest as stout as the general setae instead of only long setae on the second article of the female gonopod. A key to the Chinese species of Ezembius is presented. [ABSTRACT FROM AUTHOR]

Geographical barriers and distance can reduce gene exchange among animals, resulting in genetic divergence of geographically isolated populations. The Tibetan antelope (Pantholops hodgsonii) has a geographical range of approximately 1600 km across the Qinghai–Tibet Plateau, which comprises a series of tall mountains and big rivers. However, previous studies indicate that there is little genetic differentiation among their geographically delineated populations. To better understand the genetic structure of P. hodgsonii populations, we collected 145 samples from the 3 major calving regions, taking into consideration their various calving grounds and migration routes. We used a combination of mitochondrial sequences (Cyt b, ATPase, D‐loop and COX I) to investigate the genetic structure and the evolutionary divergence of the populations. Significant, albeit weak, genetic differentiation was detected among the 3 geographical populations. Analysis of the genetic divergence process revealed that the animals gradually entered a period of rapid genetic differentiation approximately 60 000 years ago. The calving migration of P. hodgsonii cannot be the main cause of their weak genetic structure because this cannot fully homogenize the genetic pool. Instead, the geological and climatic events as well as the coupling vegetation succession process during this period have been suggested to greatly contribute to the genetic structure and the expansion of genetic diversity. [ABSTRACT FROM AUTHOR]

Two new species of lithobiid centipedes (Hessebius prospinosa sp. nov. and Lithobius (Ezembius) maqinensis sp. nov.) and the first record of Lamyctes africanus Porath which are all females, suggesting parthenogenesis, are described based on specimens from the Qinghai-Tibet Plateau. Keys for each of the Chinese species of Hessebius, Ezembius and Lamyctes are presented. Phylogenetic analysis using a fragment (613 bp) of mitochondrial cytochrome c oxidase subunit I (COI) indicates that (1) Lithobius (Ezembius) maqinensis sp. nov. clades with L. (E.) giganteus Sseliwanoff, and (2) the specimens of Lamyctes africanus Porath from China clade with L. africanus Porath from other countries. [ABSTRACT FROM AUTHOR]

[Display omitted] • Climatically suitable habitats for threatened birds on the QTP are mainly located in the eastern region. • Over 81% of the threatened birds would experience reduction of suitable habitat area in the future. • Most threatened birds would be distributed towards higher latitudes or higher elevations. • Birds in Galliformes order would face higher extinction risk in future climate change. The Qinghai-Tibet Plateau (QTP) contains a significant amount of biodiversity which provides a wide range of habitats for a great quantity of wildlife, especially various birds, on account of the unique climatic conditions and geography. However, the QTP has shown more sensitivity and vulnerability to global climate change than other regions, and the change pose a threat to the birds. Here, we used the MaxEnt and the barycenter shift analysis for analyzing the effects of global warming on the potential future habitat of the threatened birds on the QTP by using the ACCESS-ESM1-5 global climate model of the Coupled Model Intercomparison Projects Phase 6 (cmip6) climate pattern with three Shared Socioeconomic Pathway (SSP). The results demonstrated that the climatically suitable habitats for the threatened birds are mainly distributed in the eastern and central regions of the QTP. Moreover, over 81% of the threatened birds would experience a reduction in suitable habitat area within the next 30–50 years in the different future global warming scenario. Satyr tragopan (Tragopan satyra) would lose the most habitat (57.15%), while the Tibetan babax (Pterorhinus koslowi) would lose the least (0.35%) in comparing to these birds. Nonetheless, some threatened birds showed an increase in their future habitat area because of their physiological characteristics which are more adaptable to a wide range of climate. The climatic suitable habitat for all the threatened birds, except for the Tibetan eared pheasant (Crossoptilon harmani), would move to higher altitudes or higher latitudes regions. Simultaneously, the threatened birds in the order of Galliformes had a higher risk of extinction due to their weak flight and extended migratory distances on the QTP in the future. Our study provides provide a data foundation for the conservation of biodiversity and wild birds on the QTP. [ABSTRACT FROM AUTHOR]

A railway, highway and grassland paddock fencing are the main causes of habitat fragmentation, and the fences also impair the gazelles' ability to evade predators. Przewalski's gazelle I Procapra przewalskii i is a flagship species and a Category I National Protected Wild Animal Species in China, has a restricted distribution and is endemic to the Qinghai Lake region. [Extracted from the article]

The primary host of plague in the Qinghai-Tibet Plateau (QTP), China, is Marmota himalayana, which plays an essential role in the maintenance, transmission, and prevalence of plague. To achieve a more clear insight into the differentiation of M. himalayana, complete cytochrome b (cyt b) gene and 11 microsatellite loci were analyzed for a total of 423 individuals from 43 localities in the northeast of the QTP. Phylogenetic analyses with maximum likelihood and Bayesian inference methods showed that all derived haplotypes diverged into two primary well-supported monophyletic lineages, I and II, which corresponded to the referential sequences of two recognized subspecies, M. h. himalayana and M. h. robusta, respectively. The divergence between the two lineages was estimated to be at about 1.03 million years ago, nearly synchronously with the divergence between M. baibacina and M. kastschenkoi and much earlier than that between M. vancouverensis and M. caligata. Genetic structure analyses based on the microsatellite dataset detected significant admixture between the two lineages in the mixed region, which verified the intraspecies level of the differentiation between the two lineages. Our results for the first time demonstrated the coexistence of M. h. himalayana and M. h. robusta, and also, determined the distribution range of the two subspecies in the northeast of QTP. We provided fundamental information for more effective plague control in the QTP. [ABSTRACT FROM AUTHOR]

• Both wildlife and livestock were considered to assess grazing stress to obtain accurate results. • Domestic livestock contributed more to grazing pressure than wildlife in Three-River Headwaters. • Edible forage carrying capacity is more beneficial to achieve grassland and livestock balance than crude protein carrying capacity. • Analysis of grazing pressure in Qinghai-Tibet Plateau should focus more on the selective feeding of animals and seasonal factors. Grassland is not only the natural habitats for the survival of wild animals, but also the material guarantees for livestock husbandry. However, the previous studies on grassland carrying capacity are mostly only considering livestock but wild herbivores were ignored. In this study, the population of large wild herbivores, Tibetan wild ass, Tibetan gazelle and Tibetan antelope, and livestock were surveyed, and the grassland forage yield was estimated by a remote sensing-process coupled model in Qumahe Township in Three-River Headwaters, Qinghai-Tibetan in 2020. The actual carrying capacity (AC) was calculated from the both wildlife and livestock and the theoretical carrying capacity (TC) in the term on edible herbage and crude protein was estimated. Grazing pressure index was measured by the ratio of AC to TC in this region. The results showed the AC was contributed by about 20,700 standard sheep units (SHU) from wild large herbivores, and 256,500 SHU from the livestock. The theoretical carrying capacity of edible herbage, which is 274,800 SHU, was lower than the theoretical carrying capacity of crude protein in the region. If only considering livestock, the grazing capacity pressure index is 0.93, but the index will be 1.01 if the both wildlife and livestock were considered, and wildlife contributes 7.5% to the grazing pressure. This study suggested the wildlife population should be calculated in grassland ecological carrying capacity estimation though domestic livestock contributed much more to grazing pressure than wild herbivores. The new indicator would be useful in grassland management to maintain ecosystem and biodiversity balance, and society-economy sustainability. [ABSTRACT FROM AUTHOR]

Echinococcosis is highly endemic over large parts of the Qinghai–Tibet Plateau (QTP), China. Based on a large number of samples, we present data on the current presence, host distribution, and genetic diversity of Echinococcus in the Qinghai Province, located in the northeastern corner of the QTP and constituting >25% of the area of the plateau. We used 521 samples (including 451 newly collected samples and 70 samples from our previous study) from humans, yaks, sheep, goats, dogs, fox, plateau pikas, and voles in 36 counties, and genotyped them using the mitochondrial DNA marker cytochrome oxidase subunit I (cox1) gene and the maximum parsimony and Bayesian reconstruction methods. Based on the 792 bp sequence matrix, we recorded 177 variable sites; 157 were parsimony-informative. A total of 105 haplotypes (H1–H105) were detected, of which H1–H15 and H90–H104, H16–H17, H18–H89, and H105 belonged to Echinococcus shiquicus , Echinococcus multilocularis , Echinococcus granulosus , and Echinococcus canadensis , respectively. Our results showed that, (i) the Qinghai Province was under a high burden of Echinococcus epidemiology; (ii) E. granulosus was the main echinococcosis threat to the local people, and the followed is E. multilocularis ; (iii) there are a considerable number of haplotypes shared by domestic animals (sheep, yaks, and dogs) and humans, demonstrating the close relationship between human and domestic animals epidemiology; (iv) the threat of E. shiquicus on humans and livestock can be mostly ignored, while the infection risk of E. canadensis echinococcosis should not be neglected. [ABSTRACT FROM AUTHOR]

Simple Summary: Captivity is a common conservation method for endangered animals. However, a growing number of recent studies have shown that some animals in captivity might be in sub-health condition. The gut microbiota has been described as a complex, interactive internal system that has effects on diseases of the host with many interactions, and the occurrence of certain diseases is accompanied by changes and disorder of gut microbiota. We used16S rRNA sequencing technology and a mathematical model to find differences in gut microbiota composition and assembly processes. The results show that captivity might be unfavorable for white-lipped deer by shifting the gut microbiota composition and assembly process. White-lipped deer (Cervus albirostris) is a nationally protected wild animal species in China, as well as a unique and endangered species, according to the International Union for Conservation of Nature (IUCN) Red List. Captivity may alleviate the pressure from poaching and contribute to the repopulation and conservation of the population in the wild. The gut microbiota is described as a complex, interactive internal system that has effects on diseases of the host, with many interactions. However, the influence of captivity on the composition and assembly process of gut microbiota in white-lipped deer is unclear. This study applied high-throughput 16S rRNA sequencing technology to determine differences in the gut microbiota between captive (CW) and wild (WW) white-lipped deer. We used the null model, neutral community model, and niche width to identify whether captivity affects the composition and assembly process of gut microbiota. The results show that WW has a higher number of Firmicutes and a lower number of Bacteroidetes compared with CW at the phylum level, and it has more opportunistic pathogens and specific decomposition bacteria at the genus level. Principal coordinate analysis also indicated significant differences in the composition and function of gut microbiota in CW and WW. Moreover, the results reveal that captivity shifts the ecological assembly process of gut microbiota by raising the contribution of deterministic processes. In conclusion, our results demonstrate that captivity might potentially have an unfavorable effect on white-lipped deer by continually exerting selective pressure. [ABSTRACT FROM AUTHOR]

Simple Summary: The gut microbiota are crucial for hosts. For mammals, different gastrointestinal tract (GIT) segments have specific microbial communities, which play an essential role in the host's nutrition, metabolism, immunity, and health. Plateau zokors (Eospalax baileyi) and Gansu zokors (Eospalax cansus) are closely related species that belong to the Spalacidae family, and are common pests in agriculture, forestry, and animal husbandry in northwestern China, with a sympatric distribution area in the transition zone between the Qinghai-Tibetan Plateau and the Loess Plateau. Here, the characteristics of the microbiota communities in different GIT segments of the plateau zokor and the Gansu zokor were studied, and the microbiota communities of the two zokor species were compared. Our results provide important information for further study on the function of microbiota communities in different GIT segments and the potential use of the gut microbiota as a new method for the population management of the zokors. In this study, based on high-throughput sequencing technology, the biodiversity and the community structure of microbiota in different GIT segments (the stomach, small intestine, cecum and rectum) of plateau zokors and Gansu zokors were studied and compared. A source tracking analysis for the microbial communities of different GIT segments was carried out using the fast expectation–maximization microbial source tracking (FEAST) method. We found that, for both species, the microbial community richness and diversity of the small intestine were almost the lowest while those of the cecum were the highest among the four segments of the GIT. Beta diversity analyses revealed that the bacterial community structures of different GIT segments were significantly different. As for the comparison between species, the bacterial community compositions of the whole GIT, as well as for each segment, were all significantly different. Source tracking conducted on both zokors indicated that the soil has little effect on the bacterial community of the GIT. A fairly high percentage of rectum source for the bacterial community of the stomach indicated that both zokors may engage in coprophagy. [ABSTRACT FROM AUTHOR]

The Qinghai-Tibetan Plateau (QTP, in western China), which is the largest and highest plateau on Earth, is a highly epidemic region for Echinococcus spp. We collected 70 Echinococcus samples from humans, dogs, sheep, yaks, plateau pikas, and voles in eastern and southern Qinghai and genotyped them using the mitochondrial DNA marker cytochrome oxidase subunit I gene and maximum parsimony and Bayesian reconstruction methods. Based on the 792-bp sequence matrix, we recorded 124 variable sites, of which, 115 were parsimony-informative. Thirty-four haplotypes (H1-H34) were detected, of which H1-H15, H16-H17, and H18-H34 belonged to Echinococcus shiquicus, Echinococcus multilocularis, and Echinococcus granulosus, respectively. Within 26 human isolates, three were identified as E. multilocularis and 23 were E. granulosus. We also detected a dual infection case in a dog with E. multilocularis and E. granulosus. The intraspecific haplotype (Hd ± SD) and nucleotide (Nd ± SD) diversity of E. shiquicus (0.947 ± 0.021; 0.00441 ± 0.00062) was higher than that for E. granulosus (0.896 ± 0.038; 0.00221 ± 0.00031) and E. multilocularis (0.286 ± 0.196; 0.00036 ± 0.00025). Moreover, the haplotype network of E. shiquicus showed a radial feature rather than a divergent feature in a previous study, indicating this species in the QTP has also evolved with bottleneck effects. [ABSTRACT FROM AUTHOR]

• The habitat of four antelopes showed a decreasing trend under climate change. • The four species were predicted to migrate to higher latitudes in the future. • The migration paths provide reference for the establishment of corridors. Climate change is predicted to directly or indirectly affect species distribution and abundance, especially the species that live on the Qinghai-Tibet plateau that is highly sensitive to climate change. The Przewalski's gazelle (Procapra przewalskii), the Goitered gazelle (Gazella subgutturosa), the Tibetan gazelle (Procapra picticaudata) and the Tibetan antelope (Pantholops hodgsonii) are the only four existing antelopes living on the plateau. They play indispensable roles in regulating the structure and function of the plateau ecosystem. To understand how climate change affects the spatial distribution and migration direction of these ungulates, we applied the maximum entropy (MaxEnt) model and used 82, 57, 397 and 324 GPS points of Przewalski's gazelle, Goitered gazelle, Tibetan gazelle and Tibetan antelope, respectively. These points were mainly obtained through the survey of the line transect method and a small part from the database, and then we combined with the related environmental variables, and afterwards evaluated to predict the habitat change and shift of species geographic range under three climate scenarios in the 2050s and 2070s. Additionally, the potential migration paths in the future were simulated by the Minimal Cumulative Resistance (MCR) model. The results showed that climate change would cause habitat loss for all four species. The Tibetan antelope was predicted to lose over 50% of its current inhabited area under the most severe climate scenario. Also, the suitable habitat of all species would shift to higher latitudes. In particular, the Przewalski's gazelle as an endangered species that occupies narrow habitat area would face more severe challenges in the future. Therefore, all suitable habitats should be considered as important protection areas, and our results also provide a reference for designing the optimal migration corridors for the investigated species. [ABSTRACT FROM AUTHOR]

• We assessed population density and distribution of wildlife by sample line method. • We used the suitable habitat area to calculate the number of wildlife. • The pressure index of livestock on pastures had decreased in recent years. • The wildlife population had less impact on grasslands. Grassland carrying capacity and the number of livestock and wildlife play important roles in maintaining the balance of the grassland ecosystem. In this study, we assessed the grassland-livestock balance between large wild herbivores and livestock. We used the sample line transect method to conduct a survey on population and habitat area of wildlife in Sanjiangyuan National Park, China, from 2016 to 2017 and got detailed information about the population density and distribution of wildlife. The maximum entropy model (MaxEnt) was used to calculate the suitable habitat area of large wild herbivores. In addition, we used a suitable area to calculate the number of wildlife. The results showed that the suitable habitat area of Tibetan wild ass (Equus kiang) and Tibetan gazelle (Procapra picticaudata) were 14,463.7 and 15,306.3 km2, respectively, in Maduo County. The number of Tibetan wild ass and Tibetan gazelle was 12,176 and 4,231, respectively. The results indicated that the number of livestock was higher than that of wild herbivores, and overgrazing was the main cause of grassland desertification. However, the wildlife population had little effect on grasslands. The pressure index of livestock on pastures had gradually decreased in recent years. Therefore, we advise to protect the grasslands and control the number of livestock for maintaining local ecological balance and animal husbandry development. [ABSTRACT FROM AUTHOR]

• (1) MaxEnt model was used to predict the current and future distribution of Moschus spp. • (2) Future global climate change may drive the habitat migrate of Moschus spp. • (3) Moschus spp. were expected to shrink their habitats under future climate assumptions. • (4) Moschus spp. may migrate to higher latitudes or elevations under global warming. The population of wild musk deer (Moschus spp.) has declined in recent decades and reached an endangered status in China. Global climate change may drive the extinction rate of these species. To understand the implications of global warming on the future potential space utilization and migration direction of musk deer, both the maximum entropy model and barycenter migration analysis were utilized. Five global climate models and four representative concentration pathway scenarios were considered to simulate the distribution of six species for the years 2050 and 2070. The results indicated that the suitable habitat area would decrease over the next 30 to 50 years. These decreases of suitable habitat were more significant for the Siberian musk deer (reduced by 4.98% of the land area of China), the forest musk deer (1.04%), the black musk deer (0.86%), and the Himalayan musk deer (1.82%) compared with the other two musk deer species. The area with suitable climate for the Siberian musk deer will migrate to the southwest (to higher elevations) while areas suitable for the Alpine musk deer, the Himalayan musk deer, and the Anhui musk deer would all migrate to the northeast (to higher latitudes). However, the forest musk deer and the black musk deer will not migrate in the same direction, but will mainly migrate to the west and the north, respectively. These results provide data in support for in-situ conservation, ex-situ conservation, natural reserve community, and bio-corridor construction of China's musk deer species in response to global warming. [ABSTRACT FROM AUTHOR]

Background: Gastrointestinal microbiota play an important role in animal host immunity, nutrient metabolism, and energy acquisition, and have therefore drawn increasing attentions. This study compared the diversity of the gut microbiota of both wild and captive bharals, which is an ungulate herbivore of caprid from the Qinghai-Tibet plateau. Results: The sequencing of the V4-V5 region of the 16S rRNA gene via high-throughput sequencing technology showed that the dominant bacterial phyla are Firmicutes and Bacteroides both in wild and captive bharals. However, their abundance differed significantly between groups. Firmicutes were significantly higher in wild bharals, while Bacteroides were significantly higher in captive bharals. Different diets are likely a key influencing factor in the diversity and abundance of gut microbiota in bharals. Conclusions: Changes in diets affect the diversity of gut microbiota and the relative abundance of pathogenic bacteria, increasing the risk of diseases outbreak in captive bharals. The results of this study suggest that the structure and function of the gut microbiota should be regulated via dietary intervention, accurate provision of an individualized diet, and optimization of the functional network of gut microbiota and its interaction with the host. This will improve the ex situ protection of wild animals. [ABSTRACT FROM AUTHOR]

This article documents the public availability of transcriptome sequence data and assembled, annotated unigenes for the marmot flea, Oropsylla silantiewi.1 [ABSTRACT FROM AUTHOR]