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26 results on '"Zhang, Wan-Xia"'

5. Nicotine induces a dual effect on the beige-like phenotype in adipocytes

Author

Chen Hui-Jian, Xiang Jie, Zhang Wan-Xia, Sun Ao, Li Gai-Ling, and Yan You-e

Subjects
nicotine, beige adipocytes, beige-like dysfunction, different differentiation stages, 3t3-l1, Biology (General), QH301-705.5
Abstract

Nicotine, the main component of cigarette smoke, affects white/brown adipocytes. Few studies have concentrated on beige adipocytes. In this study, 3T3-L1 cells were differentiated in the presence of nicotine (25, 50 and 100 μmol/L) during early differentiation and maintenance stages. Cell viability and the state of lipid droplets were assessed by the MTT assay and Oil Red O, respectively, and the expression of beige-related genes and proteins was examined by RT-qPCR, Western blotting and flow cytometry. Nicotine did not alter adipocyte differentiation; however, it increased the expression of peroxisome proliferator- activated receptor gamma (PPARγ) protein during early differentiation and maintenance. Nicotine treatment during early differentiation downregulated gene and protein expression of PPARγ coactivator 1-alpha (PGC-1α), uncoupling protein 1 (UCP1) and cluster of differentiation 137 (CD137), and gene expression of Cbp/p300 interacting transactivator with Glu/ Asp rich carboxy-terminal domain 1 (Cited1), transmembrane protein 26 (Tmem26), and short stature homeobox 2 (Shox2). Nicotine treatment during the maintenance stage upregulated these beige-related genes/proteins. Nicotine treatment of immature adipocytes damaged beige function through a decrease in PGC-1α/UCP1 expression, but nicotine treatment of mature adipocytes or both immature and mature cells enhanced beige functioning. Nicotine induced beige-like phenotype dysfunction in 3T3-L1 adipocytes. This process may affect thermogenesis in adipose tissue and cause a dysfunction in fat metabolism.

Published
2019
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12. Concurrent Hearing and Genetic Screening of 180,469 Neonates with Follow-up in Beijing, China

Author

Dai, Pu, primary, Huang, Li-Hui, additional, Wang, Guo-Jian, additional, Gao, Xue, additional, Qu, Chun-Yan, additional, Chen, Xiao-Wei, additional, Ma, Fu-Rong, additional, Zhang, Jie, additional, Xing, Wan-Li, additional, Xi, Shu-Yan, additional, Ma, Bin-Rong, additional, Pan, Ying, additional, Cheng, Xiao-Hua, additional, Duan, Hong, additional, Yuan, Yong-Yi, additional, Zhao, Li-Ping, additional, Chang, Liang, additional, Gao, Ru-Zhen, additional, Liu, Hai-Hong, additional, Zhang, Wei, additional, Huang, Sha-Sha, additional, Kang, Dong-Yang, additional, Liang, Wei, additional, Zhang, Ke, additional, Jiang, Hong, additional, Guo, Yong-Li, additional, Zhou, Yi, additional, Zhang, Wan-Xia, additional, Lyu, Fan, additional, Jin, Ying-Nan, additional, Zhou, Zhen, additional, Lu, Hong-Li, additional, Zhang, Xin, additional, Liu, Ping, additional, Ke, Jia, additional, Hao, Jin-Sheng, additional, Huang, Hai-Meng, additional, Jiang, Di, additional, Ni, Xin, additional, Long, Mo, additional, Zhang, Luo, additional, Qiao, Jie, additional, Morton, Cynthia Casson, additional, Liu, Xue-Zhong, additional, Cheng, Jing, additional, and Han, De-Min, additional

Published
2019
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24. Effects of prenatal and lactation nicotine exposure on glucose homeostasis, lipogenesis and lipid metabolic profiles in mothers and offspring

Author

FanThese authors contributed equally to this work., Jie, Ping, Jie, Xiang, Jie, Rao, Yi-song, Zhang, Wan-xia, Chen, Ting, Zhang, Li, and Yan, You-e

Abstract

There is increasing evidence suggesting that maternal nicotine (NIC) exposure alone can lead to many deleterious consequences in the fetus. In this study, we aimed to evaluate the effects of prenatal and lactation NIC exposure on glucose homeostasis, lipogenesis and lipid metabolism in mothers and pups. After maternal NIC exposure (from gestational day 9 to weaning), NIC mothers showed lower body weight, decreased parametrial white adipose tissue (pWAT) and inguinal WAT weights, lower homeostasis model assessment of beta cell function, higher serum total cholesterol (TC) and low-density lipoprotein cholesterol levels, higher Castelli index values, lower hepatic mRNA levels of sterol regulatory element binding protein-1c (SREBP1c), lipoprotein lipase, acetyl-CoA carboxylase, fatty acid synthase (FAS) and glucose transporters 4 (GLUT4), as well as lower SREBP1c, FAS, leptin and GLUT4 mRNA levels in pWAT. However, female NIC pups presented higher body weights and serum TC levels, and increased trends for high density lipoprotein-cholesterol and Castelli index I. Male NIC pups had higher body weight, serum TC levels and Castelli index I values, and lower glycemia levels. Additionally, hepatic and adipose FAS gene expression from the female NIC pups presented a decreasing trend, while the male NIC pups had lower hepatic FAS expression and higher adipose FAS expression. In conclusion, prenatal and lactation NIC exposure induced deleterious effects on the glucose homeostasis, lipogenesis and lipid metabolism in both mothers and pups, which may promote several important metabolic disorders in the progeny. Additionally, there are gender-specific effects on pups.

Published
2016
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25. Nucleotide Diversity in Waxy Gene and Validation of Single Nucleotide Polymorphism in Relation to Amylose Content in Chinese Microcore Rice Germplasm.

Author

Qiao Wei-Hua, Chen You-Tao, Wang Rong-Sheng, Wei Xin, Cao Li-Rong, Zhang Wan-Xia, and Yang Qing-Wen

Subjects
RICE, SINGLE nucleotide polymorphisms, AMYLOSE, PLANT proteins, PLANT germplasm, PROTEIN synthesis, GENETIC markers in plants
Abstract

The waxy protein primarily controls the synthesis of amylose, which is a key determinant of rice (Oryza sativa L.) cooking and processing qualities. Knowledge of the diversity of waxy gene in Chinese rice is essential to validate molecular markers for marker-assisted selection and to trace the origin of Chinese glutinous rice. The waxy (Wx) gene in the wild rice has rarely been studied, and the origin of Chinese glutinous rice is not well understood. The objectives of our investigation were (i) to identify the diversity of Wx and molecular markers for marker-assisted breeding and (ii) trace the origin of Chinese glutinous rice. We examined the sequence variations for the waxy gene of 98 accessions of cultivated rice and 134 accessions of wild rice from a previously established microcore collection of Chinese rice germplasm. A total of 51 and 226 single nucleotide polymorphisms (SNPs) or insertions or deletions were found in the cultivated rice and the wild rice, respectively. Wild rice accessions had much higher diversity than cultivated rice and nonglutinous rice had much higher diversity than glutinous rice whereas the genetic diversity of indica rice was similar to that of japonica rice. Polymorphisms of CTn microsatellite, G/T SNP, and 23-bp insertion in the waxy gene and their relationship to amylose content were explored using cultivated rice. The G/T allele and 23-bp insertion were better associated with amylose content than with the CTn alleles, and they were validated as molecular markers for markerassisted selection. All the wild rice accessions with 23-bp insertion or Τ allele came from South China and the glutinous rice originated from wild rice of South China parallel with indica and japonica differentiation. [ABSTRACT FROM AUTHOR]

Published
2012
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