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C20H13ClN2O3, monoclinic, P21/n (no. 14), a = 6.2256(5) Å, b = 18.3976(15) Å, c = 14.5582(15) Å, β = 90.555(8)°, V = 1667.4(3) Å3, Z = 4, R gt(F) = 0.0443, wR ref(F 2) = 0.1003, T = 153(2) K.

C26H18BrN3O2, monoclinic, P21/n (no. 14), a = 9.5191(14) Å, b = 23.056(3) Å, c = 10.0556(15) Å, β = 95.470(3)°, V = 2196.9(6) Å3, Z = 4, Rgt(F) = 0.0640, wRref(F2) = 0.1645, T = 296(2) K.

C20H13ClN2O3, monoclinic, P21/n (no. 14), a = 6.2256(5) Å, b = 18.3976(15) Å, c = 14.5582(15) Å, β = 90.555(8)°, V = 1667.4(3) Å3, Z = 4, Rgt(F) = 0.0443, wRref(F2) = 0.1003, T = 153(2) K. [ABSTRACT FROM AUTHOR]

C20H13ClN2O3, monoclinic, P21/n (no. 14), a = 6.2256(5) Å, b = 18.3976(15) Å, c = 14.5582(15) Å, β = 90.555(8)°, V = 1667.4(3) Å3, Z = 4, Rgt(F) = 0.0443, wRref(F2) = 0.1003, T = 153(2) K. [ABSTRACT FROM AUTHOR]

Objective *To examine the role of blood lipoprotein phospholipase A2 (Lp-PLA2) and neutrophil gelatinase-associated lipocalin (NGAL) in the diagnosis of early diabetic nephropathy (DN). Methods'A total of 219 participants with type 2 diabetes mellitus diagnosed in the Department of Endocrinology of Taizhou People's Hospital from January 2017 to April 2019 were enrolled. The levels of Lp-PLA2, NGAL, urinary albumin excretion rate (UAER), blood urea nitrogen (BUN), serum creatinine (Scr), uric acid (UA), cystatin C (Cys-C), blood glucose, and blood lipid in all participants were detected. All diabetics were classified into three groups based on UAER: normal albuminuria (NA) group (n=75), microalbuminuria (MA) group (n= 73) and heavy albuminuria (HA) group (n=71). The MA group and HA group were classified as DN group.The differences of the above indicators among each group were compared, and the relationship of Lp-PLA2, NGAL and DN were analyzed by Pearson linear correlation, multiple linear regression, Logistic regression and receiver operator characteristic curve. Results *Compared with the NA group and MA group, the levels of Lp-PLA2, NGAL, BUN, SCr, UA and Cys-C in the HA group were significantly increased (P<0.05). However, compared with the NA group, only Lp-PLA2 and NGAL levels in the MA group were significantly increased (P<0.05). Correlation analysis found the Lp-PLA2 and NGAL were positively correlated with UAER (r=0.397, r=0.511), BUN (r=0.274, r=0.411), SCr (r=0.237, r=0.419), and Cys-C (r=0.278, r=0.436) (all P=0.000), whereas negatively correlated with the estimate glomerular filtration rate (r= -0.170, r= -0.366; P=0.013, P=0.000). Multiple linear regression analysis showed that Lp-PLA2, NGAL and Cys-C were related factors of UAER. Binary Logistic regression analysis found that Lp-PLA2 and NGAL were still risk factors of kidney injury in DN after adjustment for blood pressure, lipids, glycosylated hemoglobin and duration of diabetes (OR=1.012, 0R=1.024; P=0.009, P=0.000). The area under the curve (AUC) of Lp-PLA2, NGAL and their combination in diagnosis of DN were 0.700, 0.855 and 0.871. NGAL demonstrated high sensitivity (81.2%) and specificity (80.0%). Lp-PLA2- and NGAL-combined detection had the highest diagnostic efficiency (AUC=0.871). Conclusion *Both blood Lp-PLA2 and NGAL levels are closely associated with the renal damage in participants with DN. As independent risk factors of DN, Lp-PLA2 and NGAL have been highly expressed in early DN, which could better reflect the occurrence of early DN, compared with traditional renal function indicators. Lp-PLA2- and NGAL-combined detection could demonstrate its maximum diagnostic efficacy, providing a new method for the diagnosis of early renal injury. [ABSTRACT FROM AUTHOR]

C20H20Br4N2O3V, monoclinic, P21/c (no. 14), a = 12.6011(10) Å, b = 16.5997(8) Å, c = 12.9206(12) Å, β = 119.006(11)°, V = 2363.7(4) Å3, Z = 4, Rgt(F) = 0.0518, wRref(F2) = 0.1062, T = 153(2) K. [ABSTRACT FROM AUTHOR]

C26H18BrN3O2, monoclinic, P21/n (no. 14), a = 9.5191(14) Å, b = 23.056(3) Å, c = 10.0556(15) Å, β = 95.470(3)°, V = 2196.9(6) Å3, Z = 4, R gt (F) = 0.0640, wR ref (F 2) = 0.1645, T = 296(2) K. [ABSTRACT FROM AUTHOR]

Objective: To establish the in vivo traceable acute myeloid leukemia mice model with Luciferase-Expressing KG1a Cells., Methods: KG1a cells with stable luciferase gene expression (called as KG1a-Luc cells) were constructed by lentivirus transfection, then sifted out by puromycin. Eighteen male NOD-SCID-IL2rg -/- mice aged 8 to 12 weeks were randomly and equally divided into two groups: the control group and the KG1a-Luc group. The mice in KG1a-Luc group were injected with 200 μl PBS containing 5×10 6 KG1a-Luc cells through tail veins, and the mice in control group were injected with 200 μl PBS only. The bioluminescence imaging technology was used to monitor the tumor burden in vivo. The peripheral blood of the mice in both groups was analyzed by flow cytometry. After the mice were sacrificed, there were pathologic evaluations: bone marrow and spleens made into smears, and livers sliced to get paraffin sections. The survival time of the mice in the two groups was recorded and compared., Results: KG1a cells expressing luciferase stably were successfully obtained. The tumor luminescence wildly spread at day 17 captured by in vivo imaging. The KG1a-Luc tumor cells could be detected in the peripheral blood of the mice, with the average percentage of (16.27±6.66)%. The morphology and pathology result showed that KG1a-Luc cells infiltrate was detected in bone marrow, spleens and livers. The survival time of the KG1a-Luc mice was notably shorter as compared with those in the control group, the median survival time was 30.5 days (95%CI: 0.008-0.260)., Conclusion: The acute myeloid leukemia NOD-SCID-IL2rg -/- mouse model was successfully established by tail vein injection of 5×10 6 KG1a-Luc cells.

MicroRNAs (miRNAs) play important roles in the development of skeletal muscle. In our previous study, expression of miR-195 and miR-497 were shown to be upregulated during muscle development in pigs. In this study, we investigated the roles of these two miRNAs in myogenesis and analyzed their transcriptional regulation. Our results showed that miR-195 and miR-497 were upregulated during muscle development and myoblast differentiation. Moreover, miR-195 and miR-497 inhibited proliferation but not differentiation in C2C12 cells. Further investigation revealed that Igf1r, Insr, Ccnd2 and Ccne1 were directly targeted by miR-195 and miR-497 in myoblasts. In addition, we confirmed that miR-195 and miR-497, which shared the similar expression profiling, were negatively regulated by nuclear factor κB (NF-κB) in both myoblasts and skeletal muscle tissue. Our data illustrate that the signaling pathway NF-κB-miR-195/497-Igf1r/Insr-Ccnd2/Ccne1 plays important roles in myogenesis. Our study provides novel evidence for the roles of miR-195 and miR-497 in muscle development., (© 2016. Published by The Company of Biologists Ltd.)

A new method for the determination of butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) in cosmetics by gas chromatography-mass spectrometry selected ion storage (SIS) method was developed. The BHA and BHT in samples were extracted by methanol. The m/z 165 and m/z 205 were the monitoring ion for BHA and BHT respectively. The detection limits of BHA and BHT in samples were 2.5 micrograms/g and 0.5 microgram/g, respectively. The method is simple, rapid and accurate.