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Glypicans are closely associated with organ development and tumorigenesis in animals. Dally-like (Dlp), a membrane-bound glypican, plays pivotal roles in various biological processes in Drosophila. In this study, we observed that an excess of Dlp led to the malformation of legs, particularly affecting the distal part. Accordingly, the leg disc was shrunken and frequently exhibited aberrant morphology. In addition, elevated Dlp levels induced ectopic cell death with no apparent cell proliferation changes. Furthermore, Dlp overexpression in the posterior compartment significantly altered Wingless (Wg) distribution. We observed a marked expansion of Wg distribution within the posterior compartment, accompanied by a corresponding decrease in the anterior compartment. It appears that excess Dlp guides Wg to diffuse to cells with higher Dlp levels. In addition, the distal-less (dll) gene, which is crucial for leg patterning, was up-regulated significantly. Notably, dachshund (dac) and homothorax (hth) expression, also essential for leg patterning and development, only appeared to be negligibly affected. Based on these findings, we speculate that excess Dlp may contribute to malformations of the distal leg region of Drosophila, possibly through its influence on Wg distribution, dll expression and induced cell death. Our research advances the understanding of Dlp function in Drosophila leg development. [ABSTRACT FROM AUTHOR]

To quickly calibrate the discrete element parameters (DEP) of pellets with different moisture content (MC), the angle of repose (AoR) was taken as the target value to conduct experimental and simulation research on gluten pellets. The experimental method obtained the intrinsic parameters, contact parameters, and AoR of pellets with different moisture content. The parameters differed significantly under different moisture content (p < 0.05). The AoR‐MC model (R2 = 0.987) was established. The Plackett–Burman test, steepest ascent test, and center compound test were carried out to establish the AoR‐DEP model (R2 = 0.969) with a relative error less than or equal to 2.07%. The MC‐DEP model was derived, and verified by the side plate lifting method with a relative error less than or equal to 2.58%. This paper provides a new method for calibrating DEP under different moisture content. [ABSTRACT FROM AUTHOR]

eBay's feedback rating system is currently widely used. In this study, we examine if eBay's feedback rating types (+, 0, −) are consistent with the sentiments reflected in the textual comments posted by buyers. Using the datasets collected from eBay, we test the hypotheses associated with the research questions at three levels: individual, group, and total. Overall, the types of feedback ratings are consistent with the sentiments embedded in the textual comments. However, there are some issues with eBay's current feedback rating system: (1) at the individual level, the correlation coefficient between the ratings and the comments' sentiments is low at 0.4311 (<0.5). While the three types of ratings are symmetric, like (−1, 0, +1), buyers' textual comments have asymmetric distributions of sentiments among these three types. The three simple feedback ratings (+, 0, −) are not fully aligned with the sentiments revealed in the textual comments posted by buyers. We propose expanding the current three ratings into five ratings such as (−2, −1, 0, +1, +2), which might help remedy the issue. We contribute to the literature by tapping into this less-studied area vital to improving the online marketplace's efficiency. [ABSTRACT FROM AUTHOR]