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Objective To investigate the influence of smoking on subgingival flora after periodontitis treatment through microbiological experiments. Methods Fifty-nine patients with periodontitis who completed periodontal treatment in the Department of Stomatology of Nanjing Jiangbei Hospital from 2019 to 2021 were selected and divided into smoking group(30 patients) and non-smoking group(29 patients). The clinical samples of patients's periodontal pockets before and after treatment were collected for detection of Actinobacillus (Aa), Porphyromonas gingivalis (Pg), Plasmobacterium intermedius (PI), Bacteroides forsythiae (Bf), Fusobacterium nucleatum (Fn) and Streptococcus minimus (Pm). Results After treatment, the bleeding index and periodontal attachment of the smoking group were better than those before treatment, while the plaque area index, bleeding index, periodontal pocket depth and periodontal attachment of the non-smoking group were better than those before treatment, and the plaque area index, periodontal pocket depth and periodontal attachment of the non-smoking group were better than those of the smoking group, with statistically significant differences(P＜0.05). After treatment, the positive rate of Pg and Pi in the smoking group was lower than that before treatment, the positive rate of Aa, Pg, Pi, Bf, Fn and Pm in the non-smoking group was lower than that before treatment, and the positive rate of Pg, Bf, Fn and Pm in the non-smoking group was lower than that in the smoking group, with statistically significant differences(P＜0.05). Conclusion Compared with smokers, non-smokers have better periodontal attachment recovery and lower detection rate of periodontal bacteria after periodontal treatment.

Objective To compare the bacteriostatic effect of two disinfections on the surface of frequently touched objects in dental clinic, so as to provide the reference for proper disinfection. Methods Specimens from the control panel and surface of examination table of comprehensive treatment chair were taken for bacterial culture, record the bac⁃ teria content on the objects surface. Then disinfect the objects surface by using 500 mg/L chlorine⁃containing disinfec⁃ tant (routing group) and Gamma disinfecting wet wipes (test group) respectively, compare the qualified rate of bacterio⁃ stasis on object surfaces between two group. Results After 10⁃minute disinfection on surfaces, bacteriostatic rate of routing group and test group was(91.66±7.52)% and (93.87±6.12)% respectively, there was no significant difference be⁃ tween two groups (P>0.05). Conclusion The quaternary ammonium disinfectant for the dental clinic objects can reach the same effect as chlorine⁃containing disinfectant.

Psathyrostachys huashanica Keng (2n = 2x = 14, NsNs), a distant wild relative of common wheat, possesses rich potentially valuable traits, such as disease resistance and more spikelets and kernels per spike, that could be useful for wheat genetic improvement. Development of wheat-P. huashanica translocation lines will facilitate its practical utilization in wheat breeding. In the present study, a wheat-P. huashanica small segmental translocation line, K-13-835-3, was isolated and characterized from the B[C.sub.1][F.sub.5] population of a cross between wheat-P. huashanica amphiploid PHW-SA and wheat cultivar CN16. Cytological studies showed that the mean chromosome configuration of K-13-835-3 at meiosis was 2n = 42 = 0.10 I + 19.43 II (ring) + 1.52 II (rod). GISH analyses indicated that chromosome composition of K-13-835-3 included 40 wheat chromosomes and a pair of wheat-P. huashanica translocation chromosomes. FISH results demonstrated that the small segment from an unidentified P. huashanica chromosome was translocated into wheat chromosome arm 5DS, proximal to the centromere region of 5DS. Compared with the cultivar wheat parent CN16, K-13-835-3 was highly resistant to stripe rust pathogens prevalent in China. Furthermore, spikelets and kernels per spike in K-13-835-3 were significantly higher than those of CN16 in two growing seasons. These results suggest that the desirable genes from P. huashanica were successfully transferred into CN16 background. This translocation line could be used as novel germplasm for high-yield and, eventually, resistant cultivar breeding. Keywords: Psathyrostachys huashanica, translocation line, stripe rust, kernels per spike. Resume : Le Psathyrostachys huashanica Keng (2n = 2x = 14, NsNs), une espece sauvage lointainement apparentee au ble, possede des caracteres potentiellement tres utiles, comme la resistance aux maladies et un plus grand nombre d'epillets et de grains par epi, lesquels pourraient s'averer utiles en amelioration genetique du ble. Le developpement de lignees avec des translocations ble-P. huashanica pourrait faciliter l'utilisation de cette espece en selection chez le ble. Dans ce travail, une lignee presentant une petite translocation d'un segment du P. huashanica chez le ble, K-13-835-3, a ete isolee et caracterisee au sein d'une population B[C.sub.1][F.sub.5] derivee d'un croisement entre un amphiploide ble-P. huashanica PHW-SA et un cultivar de ble CN16. Des analyses cytologiques ont revele que la configuration chromosomique typique chez K-13-835-3 lors de la meiose etait 2n = 42 = 0,10 I +19,43 II (anneau) + 1,52 II (batonnet). Des analyses GISH ont indique que le complement chromosomique de K-13-835-3 incluait 40 chromosomes du ble et une paire de chromosomes avec une translocation ble-P. huashanica. Des analyses FISH ont montre qu'un petit segment provenant d'un chromosome non-identifie du P. huashanica a ete transloque au sein du bras chromosomique 5DS, a proximite de la region centromerique. En comparaison avec le cultivar de ble parental CN16, la lignee K-13-835-3 s'est montree tres resistante aux agents causant la rouille jaune en Chine. De plus, le nombre d'epillets et de grains par epi chez la lignee K-13-835-3 etait significativement plus eleve que chez CN16 au cours de deux saisons. Ces resultats suggerent que des genes desirables du P. huashanica ont ete transferes avec succes chez CN16. Cette lignee a translocation pourra servir de ressource genetique afin de developper des cultivars combinant haut rendement et resistance. [Traduit par la Redaction] Mots-cles : Psathyrostachys huashanica, lignee a translocation, rouille jaune, nombre de grains pas epi., Introduction The relatively narrow range of genetic variation of wheat is the primary factor that has hampered the improvement of crop yield in recent years (Mujeeb-Kazi et al. 2013). Widening [...]

Reactive oxygen species (ROS) play multiple roles in synaptic transmission, and estrogen-related receptor α (ERRα) is involved in regulating ROS production. The purpose of our study was to explore the underlying effect of ERRα on ROS production, neurite formation and synaptic transmission. Our results revealed that knocking down ERRα expression affected the formation of neuronal neurites and dendritic spines, which are the basic structures of synaptic transmission and play important roles in learning, memory and neuronal plasticity; moreover, the amplitude and frequency of miniature excitatory postsynaptic currents (mEPSCs) and miniature inhibitory postsynaptic currents (mIPSCs) were decreased. These abnormalities were reversed by overexpression of human ERRα. Additionally, we also found that knocking down ERRα expression increased intracellular ROS levels in neurons. ROS inhibitor PBN rescued the changes in neurite formation and synaptic transmission induced by ERRα knockdown. These results indicate a new possible cellular mechanism by which ERRα affects intracellular ROS levels, which in turn regulate neurite and dendritic spine formation and synaptic transmission., Competing Interests: The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:Yuanlin Ma reports financial support was provided by 10.13039/501100001809National Natural Science Foundation of China. Demei Xu reports financial support was provided by 10.13039/501100001809National Natural Science Foundation of China. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2023 The Authors. Published by Elsevier Ltd.)

Objective: To calculate the cut-off values of speed of platelet recovery and its R-squared in patients with acute myeloid leukemia (AML) after initial induction chemotherapy, which were used to predict the complete remission (CR) of the first induction chemotherapy, and guide the clinic to choose the next appropriate chemotherapy regimen as soon as possible., Methods: A total of 117 patients with newly diagnosed AML in the Second Hospital of Shanxi Medical University were included. Patients were diagnosed by morphology, immunology, cytogenetics, and molecular biology (MICM) classification, and the risk stratification was evaluated in combination with the clinical situations of the patients at the time of admission. The peripheral platelet counts after the first induction chemotherapy were detected and the linear regression equation was used to calculate the recovery speed of platelet counts in 5 consecutive blood cell analysis before discharge. According to the ROC curve, the cut-off value between the recovery speed and the R-squared was calculated, and the cut-off value was used to divide the patients into different groups. The differences between groups were compared by Pearson χ 2 test to observe the remission effect of the first induction chemotherapy., Results: ROC curve analysis showed that the cut-off value for predicting the platelet recovery speed and its R-squared of the first induction chemotherapy to achieve remission was 4.059 5×10 9 /(L·d) and 72.7%, the sensitivity was 77% and 63.9%, the specificity was 62.5% and 67.9%, and the Youden index was 0.395 and 0.318, respectively. The patients were divided into different groups and compared according to the above cut-off values, and the results showed statistical differences ( P <0.001, P =0.001)., Conclusion: The cut-off value of platelet recovery speed and its R-squared after the first induction chemotherapy calculated by peripheral platelet count and ROC curve in AML patients can be used as an index to evaluate the remission. The faster the platelet recovery speed after chemotherapy is, the more likely patients achieve remission. The more stable the platelet recovery tendency is, the more likely patients achieve remission too.

The heavy metals in the environment led to the defect of nutrient contents in grains and metabolic disturbance seriously. In order to research the effect of heavy metal copper and cadmium on nutrient contents and heavy metal contents of wheat grain, the present paper studied the nutrition element (P, K, Ca, Mg, Na, Fe, Mn, Zn and B) and contents of heavy metal (Pb, Cr, Hg, Cu and Cd) in organs of detached wheat spikes in vitro culture by ICP-MS/ICP-AES. The results showed that the weight of grain reduced significantly with copper and cadmium treatment. Under different copper treatment, the contents of P, K, Mg, Ca, Na, Mn and Zn increased, but Fe and B contents reduced. Exogenous cadmium promoted the absorption of P, K, Ca, Mg and Mn while interrupted the absorption of Na, Fe, Zn and B. Exogenous copper and cadmium treatment reduced the Hg content in wheat grain, but the Cu and Cd contents were 55-folds and 62 folds of the national food sanitation standard. All the data showed that the effects of copper and cadmium are disadvantageous to the accumulation of nutrient and heavy metal contents.

The gaseous photocatalytic degradation of dibenzofuran, dioxins-like substance, was investigated by using TiO2, Ce3+/TiOs and Fe3+/TiO2 as photocatalysts. The batch-type photocatalytic reaction experiments were conducted, and the effects of initial concentration of reactant, humidity, circulation flow rate and light intensity on the photocatalytic degradation of dibenzofuran were studied. And the models and methods for estimating photocatalytic reaction constants and Langmuir adsorption constants were proposed. Results showed that the photocatalytic activity of the TiO2 photocatalyst was obviously improved after doping with Fe3+ and Ce3+, and the photocatalytic activity of Fe3+/TiO2 was the best in three photocatalysts. The degradation rate of dibenzofuran increased with the increase of the initial concentration of gaseous dibenzofuran in the batch-type photocatalytic reaction system. The photocatalytic degradation of gaseous dibenzofuran was enhanced when proper quantities of water vapor existed. However, it would be inhibited if there was excessive water vapor. As the circulation flow rate and the light intensity increased, the photocatalytic degradation rate of gaseous dibenzofuran also increased. The photocatalytic reaction rate coefficients of dibenzofuran on the photocatalysts TiO2, Ce3+/TiO2 and Fe3+/TiO2 were 34.54 x 10(-5), 36.23 x 10(-5) and 37.95 x 10(-4) mg x (min x m2)(-1) respectively.