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277 results on '"Zhang, Zi-Yi"'

17. Dual action of macrophage miR‐204 confines cyclosporine A‐induced atherosclerosis.

Author

Su, Jia‐Hui, Hong, Yu, Han, Cong‐Cong, Yu, Jie, Guan, Xin, Zhu, Ya‐Mei, Wang, Cheng, Ma, Ming‐Ming, Pang, Rui‐Ping, Ou, Jing‐Song, Zhou, Jia‐Guo, Zhang, Zi‐Yi, Ban, Tao, and Liang, Si‐Jia

Subjects
CYCLOSPORINE, ATHEROSCLEROSIS, ATHEROSCLEROTIC plaque, FOAM cells, MACROPHAGES, MONOCYTES, T cells
Abstract

Background and Purpose: Atherosclerosis induced by cyclosporine A (CsA), an inhibitor of the calcineurin/nuclear factor of activated T cells (NFAT) pathway, is a major concern after organ transplantation. However, the atherosclerotic mechanisms of CsA remain obscure. We previously demonstrated that calcineurin/NFAT signalling inhibition contributes to atherogenesis via suppressing microRNA‐204 (miR‐204) transcription. We therefore hypothesised that miR‐204 is involved in the development of CsA‐induced atherosclerosis. Experimental Approach: ApoE−/− mice with macrophage‐miR‐204 overexpression were generated to determine the effects of miR‐204 on CsA‐induced atherosclerosis. Luciferase reporter assays and chromatin immunoprecipitation sequencing were performed to explore the targets mediating miR‐204 effects. Key Results: CsA alone did not significantly affect atherosclerotic lesions or serum lipid levels. However, it exacerbated high‐fat diet‐induced atherosclerosis and hyperlipidemia in C57BL/6J and ApoE−/− mice, respectively. miR‐204 levels decreased in circulating monocytes and plaque lesions during CsA‐induced atherosclerosis. The upregulation of miR‐204 in macrophages inhibited CsA‐induced atherosclerotic plaque formation but did not affect serum lipid levels. miR‐204 limited the CsA‐induced foam cell formation by reducing the expression of the scavenger receptors SR‐BII and CD36. SR‐BII was post‐transcriptionally regulated by mature miR‐204‐5p via 3′‐UTR targeting. Additionally, nuclear‐localised miR‐204‐3p prevented the CsA‐induced binding of Ago2 to the CD36 promoter, suppressing CD36 transcription. SR‐BII or CD36 expression restoration dampened the beneficial effects of miR‐204 on CsA‐induced atherosclerosis. Conclusion and Implications: Macrophage miR‐204 ameliorates CsA‐induced atherosclerosis, suggesting that miR‐204 may be a potential target for the prevention and treatment of CsA‐related atherosclerotic side effects. [ABSTRACT FROM AUTHOR]

Published
2024
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19. Dual action of macrophage miR‐204 confines cyclosporine A‐induced atherosclerosis

Author

Su, Jia‐Hui, primary, Hong, Yu, additional, Han, Cong‐Cong, additional, Yu, Jie, additional, Guan, Xin, additional, Zhu, Ya‐Mei, additional, Wang, Cheng, additional, Ma, Ming‐Ming, additional, Pang, Rui‐Ping, additional, Ou, Jing‐Song, additional, Zhou, Jia‐Guo, additional, Zhang, Zi‐Yi, additional, Ban, Tao, additional, and Liang, Si‐Jia, additional

Published
2023
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21. Crocetin protects cardiomyocytes against hypoxia/reoxygenation injury by attenuating Drp1-mediated mitochondrial fission via PGC-1α

Author

MOU, You-Ling, ZHAO, Rui, LYU, Shi-Ying, ZHANG, Zi-Yi, ZHU, Mei-Fei, and LIU, Qian

Subjects
Geriatrics and Gerontology, Cardiology and Cardiovascular Medicine, Research Article
Abstract

BACKGROUND: Saffron (Crocus sativus L.) has been traditionally used as food, spice, and medicine. Crocetin (CRT), as main bioactive component of saffron, has accumulated pieces of beneficial evidence on myocardial ischemia/reperfusion (I/R) injury. However, the mechanisms are poorly explored. This study aims to investigate the effects of CRT on H9c2 cells under hypoxia/reoxygenation (H/R) and elucidated the possible underlying mechanism. METHODS: H/R attack was performed on H9c2 cells. Cell counting kit-8 was used to detect the cell viability. Cell samples and culture supernatants were evaluated via commercial kits to measure the superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, and cellular adenosine triphosphate (ATP) content. Various fluorescent probes were used to detect cell apoptosis, intracellular and mitochondrial reactive oxygen species (ROS) content, mitochondrial morphology, mitochondrial membrane potential (MMP), and mitochondrial permeability transition pore (mPTP) opening. Proteins were evaluated via Western Blot. RESULTS: H/R exposure severely reduced cell viability and increased LDH leakage. Peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α) suppression and dynamin-related protein 1 (Drp1) activation were coincided with excessive mitochondrial fission, mitochondrial permeability transition pore (mPTP) opening and mitochondrial membrane potential (MMP) collapse in H9c2 cells treated with H/R. Mitochondria fragmentation under H/R injury induced ROS over-production, oxidative stress, and cell apoptosis. Notably, CRT treatment significantly prevented mitochondrial fission, mPTP opening, MMP loss, and cell apoptosis. Moreover, CRT sufficiently activated PGC-1α and inactivated Drp1. Interestingly, mitochondrial fission inhibition with mdivi-1 similarly suppressed mitochondrial dysfunction, oxidative stress and cell apoptosis. However, silencing PGC-1α with small interfering RNA (siRNA) abolished the beneficial effects of CRT on H9c2 cells under H/R injury, accompanied with increased Drp1 and p-Drp1(ser616) levels. Furthermore, over-expression of PGC-1α with adenovirus transfection replicated the beneficial effects of CRT on H9c2 cells. CONCLUSIONS: Our study identified PGC-1α as a master regulator in H/R-injured H9c2 cells via Drp1-mediated mitochondrial fission. We also presented the evidence that PGC-1α might be a novel target against cardiomyocyte H/R injury. Our data revealed the role of CRT in regulating PGC-1α/Drp1/mitochondrial fission process in H9c2 cells under the burden of H/R attack, and we suggested that modulation of PGC-1α level may provide a therapeutic target for treating cardiac I/R injury.

Published
2023

28. Efficient Biosynthesis of Odd-Chain Fatty Acids viaRegulating the Supply and Consumption of Propionyl-CoA in Schizochytriumsp

Author

Ma, Wang, Li, Jin, Yang, Wen-Qian, Zhang, Zi-Yi, Yan, Chun-Xiao, Huang, Peng-Wei, and Sun, Xiao-Man

Abstract

Odd chain fatty acids (OCFAs) are high-value-added compounds with great application in the field of food and medicine. As an oleaginous microorganism, Schizochytriumsp. has the potential to produce OCFAs efficiently. Propionyl-CoA is used as a precursor to synthesize OCFAs through the fatty acid synthetase (FAS) pathway, so its flow direction determines the yield of OCFAs. Here, different substrates were assessed to promote propionyl-CoA supply for OCFA accumulation. Moreover, the methylmalonyl-CoA mutase (MCM) was identified as the key gene responsible for propionyl-CoA consumption, which promotes the propionyl-CoA to enter into the tricarboxylic acid cycle rather than the FAS pathway. As one of the classic B12-dependent enzymes, the activity of MCM can be inhibited in the absence of B12. As expected, the OCFA accumulation was greatly increased. However, the removal of B12caused growth limitation. Furthermore, the MCM was knocked out to block the consumption of propionyl-CoA and to maintain cell growth; results showed that the engineered strain achieved the OCFAs titer of 2.82 g/L, which is 5.76-fold that of wild type. Last, a fed-batch co-feeding strategy was developed, resulting in the highest reported OCFAs titer of 6.82 g/L. This study provides guidance for the microbial production of OCFAs.

Published
2023
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41. Solid-liquid Equilibria in the Ternary Systems (NaCl+SrCl2+H2O) and (KCl+SrCl2+H2O) at 288.15 K and 0.1 MPa

Author

Zhang Zi-Yi, Yang Sennan, Fan Rong, and LI Dongchan

Subjects
Ternary numeral system, Chemistry, Analytical chemistry, 02 engineering and technology, General Chemistry, 021001 nanoscience & nanotechnology, law.invention, 020401 chemical engineering, law, Phase (matter), 0204 chemical engineering, Solubility, Crystallization, 0210 nano-technology, Ternary operation, Solid solution, Phase diagram, Eutectic system
Abstract

Solid-liquid phase equilibria of the two ternary systems (NaCl+SrCl2+H2O) and (KCl+SrCl2+H2O) at T=288.15 K and p=0.1 MPa were studied using the isothermal dissolution equilibrium method. Solubilities of the equilibrium liquid phase were determined, and the solids were also investigated by the Schreinemaker method of wet residues. In the ternary system (NaCl+SrCl2+H2O) at 288.15 K, there is one invariant point corresponding to (NaCl+SrCl2·6H2O) and two crystallization regions corresponding to NaCl and SrCl2·6H2O. The crystallized area of SrCl2·6H2O decreased with the increasing temperature, while that of NaCl increased slightly. In the ternary system (KCl+SrCl2+H2O) at 288.15 K, there is one invariant point(KCl+SrCl2·6H2O) and two crystallization regions cor-responding to KCl and SrCl2·6H2O. Both systems belong to a simple eutectic type, and neither double salts nor solid solutions were formed. On the basis of Pitzer-Harvie-Weare model, the solubilities of the two systems at 288.15 K were demonstrated. A comparison showed that the calculated solubilities agreed well with the experimental data.

Published
2018

48. Spin Crossover OFF/ON Triggered by Ligand Chemical Doping in an Fe(III) Solid Solution†.

Author

Peng, Shuang, Gao, Yue, Zhang, Zi‐Yi, Xu, Guang‐Yan, Zheng, Wen‐Jun, Yang, Feng‐Lei, Dai, Jing‐Wei, and Li, Zhao‐Yang

Subjects
*DENSITY functional theory, *LIGANDS (Chemistry), *ELECTROLUMINESCENT devices, *TRANSITION temperature, *DOPING agents (Chemistry)
Abstract

Comprehensive Summary Spin crossover (SCO), characterized by distinct high‐spin (HS) and low‐spin (LS) states, has potential applications in memory, electronic, and electroluminescent devices. The OFF/ON switching of SCO is crucial for obtaining bistable magnetic properties. However, there are few strategies for achieving this switching. Herein, based on a ligand chemical doping strategy, we report an Fe(III) solid solution that can be prepared using a ligand chemical doping strategy, enabling not only the OFF/ON switching of SCO but also the fine‐tuning of the spin transition temperature (Tc) within a 45 K range near room temperature. The experimental results show that when the polar ligand doping ratio reaches 20%, SCO behavior is triggered, and the crystal phase transforms significantly, becoming loose and flexible. Furthermore, Tc can be continuously regulated as the ligand‐doping ratio increases. Density functional theory (DFT) calculations reveal that solid packing‐induced molecular distortion blocks SCO, whereas loosely flexible packing triggers SCO via fluorinated ligand chemical doping. [ABSTRACT FROM AUTHOR]

Published
2024
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