16 results on '"Zhang Ya-pu"'
Search Results
2. Diabetes mellitus with a duration of 26 years combined with IgA nephropathy: A case report and literature review
- Author
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Gou, Yan-Li, primary, Zhang, Ya-Pu, additional, Xu, Yang, additional, Li, Jian-Dong, additional, Huang, Yang, additional, and Chen, Hang, additional
- Published
- 2022
- Full Text
- View/download PDF
3. Characteristics of and Virulence Factors Associated with Biofilm Formation in Clinical Enterococcus faecalis Isolates in China
- Author
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Jin-Xin Zheng, Yang Wu, Zhi-Wei Lin, Zhang-Ya Pu, Wei-Ming Yao, Zhong Chen, Duo-Yun Li, Qi-Wen Deng, Di Qu, and Zhi-Jian Yu
- Subjects
Enterococcus faecalis ,biofilm formation ,virulence genes ,multilocus sequence typing ,linezolid resistance ,Microbiology ,QR1-502 - Abstract
Enterococcus faecalis biofilm traits and distribution characteristics in China have not been clarified. This study aimed to determine the prevalence and characteristics of E. faecalis biofilm formation in a sample of clinical isolates and to explore the virulence factors associated with biofilm formation in those isolates. A total of 265 E. faecalis isolates were collected from patients in Shenzhen, China. Virulence genes were detected within the genomes of the microbes by polymerase chain reaction. The isolates were subjected to multilocus sequence typing (MLST) based on housekeeping genes. Biofilms were detected by crystal violet staining. The expression levels of the clinical E. faecalis isolates’ genes were determined by quantitative real-time polymerase chain reaction. The prevalence of biofilm formation among E. faecalis clinical isolates was 47.2%. MLST yielded 44 different sequence types (STs). The main STs were ST16 and ST179; the ST16 isolates were more likely to form strong or medium biofilm than the ST179 isolates (p < 0.001). Strong or medium biofilm formation was more common in linezolid-resistant isolates than in linezolid-sensitive isolates (p = 0.001). Biofilm formation was more frequently detected in enterococcal surface protein (esp+), surface aggregating protein (asa1+), cytolysin A (cylA+), or aggregation substance (agg+) positive isolates than in isolates that were negative (-) for these virulence factors. Multivariate regression analysis indicated that cylA [odds ratio (OR) 4.083, p < 0.001] was a risk factor for weak biofilm formation, and that esp (OR 8.207, p < 0.001) was a risk factor for strong or medium biofilm formation. The expression of cylA was raised (4.02 to 6.00-fold) in weak biofilm isolates compared to the biofilm-negative isolates, and the expression of esp was greatly elevated (11.39 to 134.08-fold) in strong biofilm isolates compared to biofilm-negative isolates. In conclusion, the ST16 classification and linezolid resistance were positively associated with strong/medium biofilm formation in clinical E. faecalis isolates. cylA was associated with weak biofilm formation, and esp was only associated with strong or medium biofilm formation of the clinical E. faecalis isolates.
- Published
- 2017
- Full Text
- View/download PDF
4. Experimental evaluation on well pattern adaptability of ultra-low permeability reservoir using sandstone flat model
- Author
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Xiao, Qian-hua / 肖前华, Wei, Guo-qi / 魏国齐, Yang, Zheng-ming / 杨正明, Xu, Xuan / 徐轩, Tian, Wen-bo / 田文博, and Zhang, Ya-pu / 张亚蒲
- Published
- 2014
- Full Text
- View/download PDF
5. Linezolid Consumption Facilitates the Development of Linezolid Resistance in Enterococcus faecalis in a Tertiary-Care Hospital: A 5-Year Surveillance Study
- Author
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Zhijian Yu, Jinxin Zheng, Lixia Xie, Qiwen Deng, Hang Cheng, Zhang-Ya Pu, Weiming Yao, Kaitao Hu, Jun Zeng, Bing Bai, Zhiwei Lin, Duoyun Li, Zhong Chen, and Weiguang Pan
- Subjects
Microbiology (medical) ,medicine.medical_specialty ,Surveillance study ,medicine.drug_class ,Immunology ,Antibiotics ,Microbiology ,Enterococcus faecalis ,03 medical and health sciences ,chemistry.chemical_compound ,Internal medicine ,Medicine ,Linezolid resistance ,030304 developmental biology ,Pharmacology ,0303 health sciences ,biology ,030306 microbiology ,business.industry ,biochemical phenomena, metabolism, and nutrition ,Tertiary care hospital ,biology.organism_classification ,chemistry ,Linezolid ,business - Abstract
Although case reports and clinical studies of linezolid (LZD)-resistant Enterococcus faecalis (LREF) have gradually increased in recent years, the relationship between LZD resistance and antibiotic...
- Published
- 2019
6. Vascular endothelial growth factor receptor-2 and its association with tumor immune regulatory gene expression in hepatocellular carcinoma
- Author
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Zhang-Ya Pu, Ze-Long Liu, Jiang Chen, Ling-Ling Zhu, Jing-Hua Liu, and Zhi-Ping Ruan
- Subjects
Sorafenib ,Vascular Endothelial Growth Factor A ,Aging ,Carcinoma, Hepatocellular ,medicine.medical_treatment ,Biology ,Immune system ,Atezolizumab ,VEGF Signaling Pathway ,medicine ,Tumor Microenvironment ,Humans ,cancer ,Growth factor ,Gene Expression Profiling ,Liver Neoplasms ,Kinase insert domain receptor ,Cell Biology ,Immunotherapy ,biochemical phenomena, metabolism, and nutrition ,Vascular Endothelial Growth Factor Receptor-2 ,Immune checkpoint ,Gene Expression Regulation, Neoplastic ,Cancer research ,bacteria ,immunotherapy ,Transcriptome ,medicine.drug ,Research Paper - Abstract
Anti-vascular endothelial growth factor (anti-VEGF) drugs have long been the only first-line treatment for advanced or unresectable hepatocellular carcinoma (HCC). Recently, the combination of bevacizumab (an anti-VEGF drug) and atezolizumab (an immune checkpoint blockade, ICB) has been proven to have superior efficacy over sorafenib. However, the complex association between VEGF signaling pathway and tumor immune microenvironment is still largely unknown. Here, we analyzed the RNA sequencing and clinical data of 365 HCC patients obtained from The Cancer Genome Atlas to investigate the potential correlation between VEGF signaling pathway and tumor immune microenvironment, including immune cell infiltration, 66 immune markers, genomic instability, and immune-related pathways. Our study revealed that VEGF signaling pathway score was positively correlated with immune cell infiltration and the expression profile of 66 immune markers. Enrichment analysis indicated that genes differentially expressed between two VEGF score subtypes were enriched in many immune-related Gene Ontology terms. Most importantly, both VEGF signaling pathway and activated CD8+ T cells were positively correlated with prognosis. Our findings suggest the co-activation of VEGF signaling pathway and tumor immune microenvironment in HCC patients, indicating the underlining mechanism of combination therapy including anti-VEGF drugs and ICBs.
- Published
- 2020
7. Characterization of biofilm formation by Enterococcus faecalis isolates derived from urinary tract infections in China
- Author
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Zhijian Yu, Duoyun Li, Qiwen Deng, Zhong Chen, Zhiwei Lin, Weiming Yao, Xiangbin Deng, Jinxin Zheng, Bing Bai, and Zhang-Ya Pu
- Subjects
0301 basic medicine ,Microbiology (medical) ,biology ,030106 microbiology ,Biofilm ,Virulence ,General Medicine ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,Microbiology ,Enterococcus faecalis ,Staining ,Housekeeping gene ,03 medical and health sciences ,Multilocus sequence typing ,Cytolysin ,Gene - Abstract
Purpose. This study explored the prevalence and characteristics of Enterococcus faecalis biofilm formation by urinary tract infection (UTI) isolates in order to identify virulence factors associated with biofilm formation.Methodology. A total of 113 E. faecalis isolates were collected from UTI patients in Shenzhen, China. The isolates were subjected to multilocus sequence typing based on housekeeping genes. Biofilms were detected by crystal violet staining and the expression levels of the E. faecalis genes were detected by quantitative real-time PCR.Results/Key findings. The main sequence types (STs) were ST16 and ST179 with the ST16 isolates more likely to form strong biofilms than the ST179 isolates (P=0.008). Strong biofilm formation was more frequently detected in aggregation substance (agg)-positive (+) isolates than in negative (-) isolates (P=0.033). Biofilm formation was also more common in isolates containing enterococcal surface protein (esp), or cytolysin A (cylA)-positive (+) isolates than in isolates negative (-) for these virulence factors. Multivariate regression analysis indicated that cylA [odds ratio (OR), 7.143, P=0.012] was associated with weak biofilm formation, and that agg (OR, 4.471, P=0.004) was associated with strong biofilm formation. The expression of cylA was increased (8.75- to 23.05-fold) in weak biofilm, and the expression of agg was greatly elevated (11.99- to 439.10-fold) in strong biofilm isolates when compared to biofilm-negative isolates.Conclusion. ST16 classification was positively associated with strong biofilm formation in E. faecalis as was agg, while cylA was associated with weak biofilm formation.
- Published
- 2018
8. Characteristics of and Virulence Factors Associated with Biofilm Formation in Clinical Enterococcus faecalis Isolates in China
- Author
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Zhang-Ya Pu, Weiming Yao, Yang Wu, Qiwen Deng, Zhijian Yu, Zhiwei Lin, Di Qu, Zhong Chen, Jinxin Zheng, and Duoyun Li
- Subjects
0301 basic medicine ,Microbiology (medical) ,030106 microbiology ,lcsh:QR1-502 ,Virulence ,multilocus sequence typing ,Microbiology ,Enterococcus faecalis ,lcsh:Microbiology ,law.invention ,03 medical and health sciences ,law ,Gene ,Polymerase chain reaction ,Original Research ,biology ,linezolid resistance ,virulence genes ,Biofilm ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,Housekeeping gene ,030104 developmental biology ,biofilm formation ,Multilocus sequence typing ,Cytolysin - Abstract
Enterococcus faecalis biofilm traits and distribution characteristics in China have not been clarified. This study aimed to determine the prevalence and characteristics of E. faecalis biofilm formation in a sample of clinical isolates and to explore the virulence factors associated with biofilm formation in those isolates. A total of 265 E. faecalis isolates were collected from patients in Shenzhen, China. Virulence genes were detected within the genomes of the microbes by polymerase chain reaction. The isolates were subjected to multilocus sequence typing (MLST) based on housekeeping genes. Biofilms were detected by crystal violet staining. The expression levels of the clinical E. faecalis isolates’ genes were determined by quantitative real-time polymerase chain reaction. The prevalence of biofilm formation among E. faecalis clinical isolates was 47.2%. MLST yielded 44 different sequence types (STs). The main STs were ST16 and ST179; the ST16 isolates were more likely to form strong or medium biofilm than the ST179 isolates (p < 0.001). Strong or medium biofilm formation was more common in linezolid-resistant isolates than in linezolid-sensitive isolates (p = 0.001). Biofilm formation was more frequently detected in enterococcal surface protein (esp+), surface aggregating protein (asa1+), cytolysin A (cylA+), or aggregation substance (agg+) positive isolates than in isolates that were negative (-) for these virulence factors. Multivariate regression analysis indicated that cylA [odds ratio (OR) 4.083, p < 0.001] was a risk factor for weak biofilm formation, and that esp (OR 8.207, p < 0.001) was a risk factor for strong or medium biofilm formation. The expression of cylA was raised (4.02 to 6.00-fold) in weak biofilm isolates compared to the biofilm-negative isolates, and the expression of esp was greatly elevated (11.39 to 134.08-fold) in strong biofilm isolates compared to biofilm-negative isolates. In conclusion, the ST16 classification and linezolid resistance were positively associated with strong/medium biofilm formation in clinical E. faecalis isolates. cylA was associated with weak biofilm formation, and esp was only associated with strong or medium biofilm formation of the clinical E. faecalis isolates.
- Published
- 2017
- Full Text
- View/download PDF
9. Characterization of biofilm formation by
- Author
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Jin-Xin, Zheng, Bing, Bai, Zhi-Wei, Lin, Zhang-Ya, Pu, Wei-Ming, Yao, Zhong, Chen, Duo-Yun, Li, Xiang-Bin, Deng, Qi-Wen, Deng, and Zhi-Jian, Yu
- Subjects
Clinical Microbiology ,virulence genes ,Enterococcus faecalis ,urinary-tract infections ,multilocus sequence typing ,biochemical phenomena, metabolism, and nutrition ,biofilm ,Research Article - Abstract
Purpose This study explored the prevalence and characteristics of Enterococcus faecalis biofilm formation by urinary tract infection (UTI) isolates in order to identify virulence factors associated with biofilm formation. Methodology A total of 113 E. faecalis isolates were collected from UTI patients in Shenzhen, China. The isolates were subjected to multilocus sequence typing based on housekeeping genes. Biofilms were detected by crystal violet staining and the expression levels of the E. faecalis genes were detected by quantitative real-time PCR. Results/Key findings The main sequence types (STs) were ST16 and ST179 with the ST16 isolates more likely to form strong biofilms than the ST179 isolates (P=0.008). Strong biofilm formation was more frequently detected in aggregation substance (agg)-positive (+) isolates than in negative (−) isolates (P=0.033). Biofilm formation was also more common in isolates containing enterococcal surface protein (esp), or cytolysin A (cylA)-positive (+) isolates than in isolates negative (−) for these virulence factors. Multivariate regression analysis indicated that cylA [odds ratio (OR), 7.143, P=0.012] was associated with weak biofilm formation, and that agg (OR, 4.471, P=0.004) was associated with strong biofilm formation. The expression of cylA was increased (8.75- to 23.05-fold) in weak biofilm, and the expression of agg was greatly elevated (11.99- to 439.10-fold) in strong biofilm isolates when compared to biofilm-negative isolates. Conclusion ST16 classification was positively associated with strong biofilm formation in E. faecalis as was agg, while cylA was associated with weak biofilm formation.
- Published
- 2017
10. Bloodstream infections caused by Enterococcus spp: A 10-year retrospective analysis at a tertiary hospital in China
- Author
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Hui Li, Xiangbin Deng, Xiaojun Liu, Qiwen Deng, Zhijian Yu, Jinxin Zheng, Zhang-Ya Pu, and Hongyan Wang
- Subjects
0301 basic medicine ,Adult ,Male ,medicine.medical_specialty ,Vascular catheter ,030106 microbiology ,Enterococcus faecium ,Biomedical Engineering ,Bacteremia ,Biochemistry ,Enterococcus faecalis ,Microbiology ,Biomaterials ,Tertiary Care Centers ,03 medical and health sciences ,Enterococcus gallinarum ,Risk Factors ,Internal medicine ,Genetics ,medicine ,Retrospective analysis ,Enterococcus casseliflavus ,Enterococcus spp ,Humans ,Gram-Positive Bacterial Infections ,Aged ,Retrospective Studies ,Cross Infection ,biology ,business.industry ,biochemical phenomena, metabolism, and nutrition ,Middle Aged ,bacterial infections and mycoses ,biology.organism_classification ,Antimicrobial ,Female ,business ,Enterococcus - Abstract
In order to discover the risk factors for 30-day mortality in bloodstream infections (BSI) caused by Enterococcus spp. strains, we explored the clinical and therapeutic profile of patients with Enterococcus spp. BSI and the characteristics of this condition. A total of 64 patients with BSI caused by Enterococcus spp. who were treated in our hospital between 2006 and 2015 were included in the study. The clinical features of patients, microbiology, and 30-day mortality were collected from the electronic medical records database and analyzed. The results showed that there were 38 patients infected by Enterococcus faecalis (E. faecalis), 24 by Enterococcus faecium (E. faecium), 1 by Enterococcus casseliflavus (E. casseliflavus), and 1 by Enterococcus gallinarum (E. gallinarum). A Charlson comorbidity score ≥5, corticosteroid treatment, placement of catheters or other prosthetic devices and history of antibiotic use were found more frequently in E. faecium BSI patients than in E. faecalis patients (P=0.017, P=0.027, P=0.008 and P=0.027, respectively). Furthermore, the univariate and multivariate analysis showed that corticosteroid treatment (OR=17.385, P=0.008), hospital acquisition (OR=16.328, P=0.038), and vascular catheter infection (OR=14.788, P=0.025) were all independently associated with 30-day mortality. Our results indicate that E. faecalis and E. faecium are two different pathogens with unique microbiologic characteristics, which cause different clinical features in BSI, and the empiric antimicrobial treatments are paramount for patients with enterococcal BSI.
- Published
- 2016
11. Study on Micro Pore Structure Characteristics of Carbonate Cores of Low Permeability
- Author
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Xiao, Pu Fu, primary, Yang, Zheng Ming, additional, Zhang, Ya Pu, additional, and Gai, Chang Cheng, additional
- Published
- 2014
- Full Text
- View/download PDF
12. Formulation for shielding effectiveness analysis of a rectangular enclosure with an electrically large aperture
- Author
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Zhao Meng, Da Xin-Yu, Zhang Ya-Pu, and Zhu Yang-Kun
- Subjects
Physics ,Acoustics ,Electromagnetic shielding ,Enclosure ,General Physics and Astronomy ,Large aperture - Abstract
Since electric components and printed circuit board in the enclosure can be destroyed by electromagnetic pulse weapons through “front door and back door” coupling, which is a great threat to the operational security, the study of the shielding effectiveness is of important significance. A formulation for shielding effectiveness analysis of a rectangular enclosure with an electrically large aperture is proposed in this paper. Firstly, the plane wave with oblique incidence and polarization is decomposed. Secondly, based on the Cohn model, the equivalent electric and magnetic dipole of the electrically large aperture is computed. Thirdly, the total Hertz electric and magnetic vector potential is obtained through mirror procedure. Finally, the electric field inside an enclosure with electrically large aperture is formulated, which is used for shielding effectiveness calculation. Five verification experiments are designed. Simulation result shows that the mean square error and absolute error of this method compared to computer simulation technology (CST) microwave studio are 11.565 dB and 8.015 dB respectively, the correlation coefficient is 0.921, through which the accuracy of this method is verified. The simulation time of this method is 0.183 s, which is only 1/7530 times of CST, so its efficiency is obvious.
- Published
- 2014
13. Available bandwidth measurement accuracy based on non-fluid model
- Author
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ZHAO, Wei-hu, primary, MENG, Xiang-ru, additional, ZHANG, Ya-pu, additional, and MA, Hai-yuan, additional
- Published
- 2011
- Full Text
- View/download PDF
14. Network fault detection based on fuzzy one class SVM with least squares and equality constraints
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ZHANG, Li, primary, MENG, Xiang-ru, additional, and ZHANG, Ya-pu, additional
- Published
- 2010
- Full Text
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15. An Aperture Scattering Model for BLT Equation Based on Least Square Support Vector Machine.
- Author
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ZHANG Ya-pu, DA Xin-yu, and XIE Tie-cheng
- Subjects
LEAST squares ,SUPPORT vector machines ,SCATTERING (Mathematics) ,ELECTROMAGNETISM ,COMPUTER algorithms - Abstract
The analysis on aperture scattering is an important composition of electromagnetic protection research on complex electronic system. A mathematic model of aperture scattering was built on the base of two-port network theory and electromagnetic topology theory, with which a generalized scatter matrix was designed. A data sieving strategy was formulized for least square support vector machine, based on which a new algorithm for aperture scattering matrix with evolving ability was proposed. By use of the simulation data of an rectangle aperture cavity's HFSS model, the aperture scattering model was tested. The mean absolute error of the forecasting about reflection and transmission are ±1.2 mV/m and ±3. 3 mV/m, while the mean relative error are ±0.17% and ±8.29%. At the precision of 90%, the absolute error of forecasting about reflection and transmission are less than and, while the relative error are less than ±0.39% and ±0.93%, which means the aperture scattering is predicted accurately. The practicability and reliability of the method are proved. [ABSTRACT FROM AUTHOR]
- Published
- 2014
16. Acute renal failure caused by Sjögren's syndrome and rheumatoid arthritis overlap syndrome.
- Author
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Ran L, Zhang YP, Guo L, Wang ZM, and Zhang JM
- Abstract
Introduction: Sjögren's syndrome (SS) and rheumatoid arthritis (RA) are two chronic autoimmune diseases. To date, there have been few reports on the overlap between SS and RA in China, especially regarding correlated acute renal failure cases., Methods: To provide a reference for our clinical peers, this article presents the case report of an elderly female patient who was diagnosed with acute renal failure caused by SS and RA overlap syndrome., Results: We also provide a relevant analysis of SS and RA overlap syndrome treatment., Conclusions: We also provide a relevant analysis of SS and RA overlap syndrome treatment., Competing Interests: The authors declare no conflict of interest., (Copyright: © 2024 Termedia & Banach.)
- Published
- 2024
- Full Text
- View/download PDF
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